MD14G1035300.v1.1

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002841
Physical Location & Seq
Reverse (-)
3150876 .. 3154933
4058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1035300.v1.1.491

Sequence Viewer

Length: 1536 bp
ATGCTCGGTCTGACTGCCTCTCTGGCCGCCGCAACGCCACCCAACGCCACCGCCACAAATTCTTGTATCATTCCAAACACCCGTCTTCTCCAGCTATCCCCCGACGCGCAAATACGAACCAATTCCTCATCGTCTTTCACGTGTTGCTCAGTGAGGAACTTGCACTCAAGTTGCAGAACAATTAAAGCCATGGCGGCGGCTGAGGAAACCCTTACAGTCACTAACCCTCCCTCACCCGTTTCCGGTAATAGGCAGGCTCTGATATCATTGTCAGACAAGAAGGATCTTGCTGTTTTAGGGAATGGGCTTCAGGACTTGGGATATCAAATTGTTTCGACTGGAGGAACTGCATCTGCCTTGTCCAATAATCACAAAGATGTCTTGGTGGTGGTTGATTCAGAAGACTATCCCGCACTTCTTGAGTTTCTTAAGGGAGACAAGGATGATCAACAATTCCGAAGGAAGCTTGCATGGAAGGCTTTCCAACATGTAGCTTCTTATGATTCTGCAGTTTCAGAATGGCTTTGGAAGCAGACTTCTGAAGATAAATTCCCTCAGAGCTTAACAGTGCCTATGTTGCTTAAAAGTCCCCTCCGTTATGGTGAAAATCCTCATCAGAAGGCTGCATTCTACGTTGACAAGAGTCTTTCTGAGGTGAATGCAGGTGGTATTGCAACTGCTATCCAACACCATGGGAAGGAAATGTCATATAATAATTATTTAGATGCCGATGCGGCTTGGAATTGTGTGTCAGAGTTTCAGAATTCTACCTGTGTAGTTGTAAAGCATACAAATCCTTGTGGCGTAGCTTCACGTGATGATATTCTTGAAGCATACAGGCTTGCTGTTAAAGCAGATCCCGTGAGTGCATTTGGTGGCATCGTAGCGTTCAACGTAGAGGTTGATGAGGCTCTTGCTAAGGAAATTCGAGAGTTTAGAAGCCCGACAGATGGCGAAACCCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTACACAAAGAAAGGGCTTGAAATCCTCCGCGGAAAATCAAAGACTCTAAGGATCCTTGAGGCAAGAAAAAATGAAAAAGGAAAGCTTTCACTTAGACAGGTTGGTGGTGGGTGGTTAGCCCAGGACTCGGATGATTTGACCCCCCAGGATATACAGTTTAAATCTGTCACTGAGAAGGCTCCACAAAAGAGTGAGCTTGCCGATGCAGAGTTCGCATGGTTGTGCGTCAAGCATGTCAAAAGCAATGCAATTGTAATAGCAAAGAATAACTGCATGCTGGGCATGGGGAGCGGGCAGCCTAACCGCTTGGAGAGTTTAAGAATAGCCTTGAAGAAAGCTGGAGAAGAGGTCAAGGGTGCAGCTTTGGCCAGCGACGCCTTCTTCCCATTCGCATGGAAAGATGCAGTGGAAGAAGCTTGTGAAAGCGGAGTTAGCGTAATTACAGAACCCGGTGGGAGCATTAGAGATGGGGATGCTATAGACTGCTGTAACAAGTATGGAGTTTCACTTCTTTTCACAAACGTTAGGCACTTCAGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

512

Amino Acids

55.64

Weight (kDa)

6.58

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AICARFT_IMPCHas PF01808 122 - 443 6.3e-113 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 1284
AccII CGCG 2 cut(s) 107, 1023
AclI AACGTT 1 cut(s) 1515
AclWI GGATC 4 cut(s) 291, 851, 1039, 1052
AcoI YGGCCR 2 cut(s) 24, 1359
AcsI RAATTY 4 cut(s) 58, 548, 763, 924
AcuI CTGAAG 3 cut(s) 293, 561, 1510
AcvI CACGTG 2 cut(s) 141, 815
AcyI GRCGYC 1 cut(s) 1368
AfaI GTAC 1 cut(s) 995
AfiI CCNNNNNNNGG 5 cut(s) 242, 249, 697, 950, 1120
AflII CTTAAG 1 cut(s) 428
AflIII ACRYGT 2 cut(s) 140, 487
AgsI TTSAA 4 cut(s) 830, 892, 1013, 1324
AjnI CCWGG 2 cut(s) 1113, 1137
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 4 cut(s) 291, 851, 1039, 1052
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 2 cut(s) 24, 1359
ApeKI GCWGC 3 cut(s) 623, 1288, 1352
ApoI RAATTY 4 cut(s) 58, 548, 763, 924
Asp700I GAANNNNTTC 3 cut(s) 121, 479, 1078
AspLEI GCGC 1 cut(s) 109
AsuC2I CCSGG 2 cut(s) 961, 1443
AsuHPI GGTGA 3 cut(s) 225, 614, 667
BalI TGGCCA 1 cut(s) 1361
BamHI GGATCC 1 cut(s) 1044
BbrPI CACGTG 2 cut(s) 141, 815
BbsI GAAGAC 2 cut(s) 77, 408
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 3 cut(s) 610, 1300, 1364
BccI CCATC 2 cut(s) 944, 1454
BciT130I CCWGG 2 cut(s) 1115, 1139
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 2 cut(s) 961, 1443
BcoDI GTCTC 1 cut(s) 429
BfmI CTRYAG 2 cut(s) 507, 1470
BfrI CTTAAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 653
BglI GCCNNNNNGGC 3 cut(s) 23, 194, 734
BisI GCNGC 8 cut(s) 27, 30, 195, 198, 624, 735, 1289, 1353
BlsI GCNGC 8 cut(s) 28, 31, 196, 199, 625, 736, 1290, 1354
Bme1390I CCNGG 4 cut(s) 961, 1115, 1139, 1443
BmiI GGNNCC 2 cut(s) 1046, 1173
BmrFI CCNGG 4 cut(s) 961, 1115, 1139, 1443
BmsI GCATC 7 cut(s) 359, 715, 721, 888, 1186, 1384, 1456
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 2 cut(s) 77, 408
BpmI CTGGAG 3 cut(s) 74, 360, 1353
Bpu10I CCTNAGC 2 cut(s) 201, 918
BpuEI CTTGAG 3 cut(s) 151, 440, 1070
BpuMI CCSGG 2 cut(s) 961, 1443
BsaAI YACGTR 2 cut(s) 141, 815
BsaHI GRCGYC 1 cut(s) 1368
BsaJI CCNNGG 5 cut(s) 189, 691, 1021, 1113, 1137
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 6 cut(s) 211, 241, 1326, 1356, 1413, 1443
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 249, 697, 950, 1120
Bse1I ACTGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 1243
BseBI CCWGG 2 cut(s) 1115, 1139
BseDI CCNNGG 5 cut(s) 189, 691, 1021, 1113, 1137
BseGI GGATG 4 cut(s) 448, 969, 1129, 1471
BseLI CCNNNNNNNGG 5 cut(s) 242, 249, 697, 950, 1120
BseMI GCAATG 1 cut(s) 1243
BseMII CTCAG 5 cut(s) 162, 192, 569, 642, 1155
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 3 cut(s) 610, 1300, 1364
BseYI CCCAGC 1 cut(s) 1270
BsgI GTGCAG 1 cut(s) 1371
Bsh1236I CGCG 2 cut(s) 107, 1023
BshFI GGCC 2 cut(s) 26, 1361
BsiSI CCGG 3 cut(s) 243, 961, 1443
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 5 cut(s) 242, 249, 697, 950, 1120
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 2 cut(s) 626, 664
BsnI GGCC 2 cut(s) 26, 1361
Bsp143I GATC 4 cut(s) 283, 445, 856, 1044
Bsp19I CCATGG 2 cut(s) 189, 691
BspANI GGCC 2 cut(s) 26, 1361
BspCNI CTCAG 5 cut(s) 161, 193, 568, 643, 1156
BspFNI CGCG 2 cut(s) 107, 1023
BspLI GGNNCC 2 cut(s) 1046, 1173
BspMAI CTGCAG 1 cut(s) 511
BspMI ACCTGC 1 cut(s) 653
BspPI GGATC 4 cut(s) 291, 851, 1039, 1052
BspTI CTTAAG 1 cut(s) 428
BsrBI CCGCTC 1 cut(s) 1284
BsrDI GCAATG 1 cut(s) 1243
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 5 cut(s) 189, 691, 1021, 1113, 1137
BssMI GATC 4 cut(s) 283, 445, 856, 1044
BssNI GRCGYC 1 cut(s) 1368
BssT1I CCWWGG 2 cut(s) 189, 691
Bst2UI CCWGG 2 cut(s) 1115, 1139
Bst4CI ACNGT 3 cut(s) 217, 568, 1149
Bst6I CTCTTC 1 cut(s) 1332
BstACI GRCGYC 1 cut(s) 1368
BstAFI CTTAAG 1 cut(s) 428
BstBAI YACGTR 2 cut(s) 141, 815
BstC8I GCNNGC 7 cut(s) 255, 468, 843, 1191, 1268, 1286, 1363
BstDEI CTNAG 8 cut(s) 148, 201, 555, 651, 918, 1040, 1085, 1164
BstDSI CCRYGG 3 cut(s) 189, 691, 1021
BstF5I GGATG 4 cut(s) 448, 969, 1129, 1471
BstFNI CGCG 2 cut(s) 107, 1023
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 4 cut(s) 286, 448, 859, 1047
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 4 cut(s) 283, 445, 856, 1044
BstNI CCWGG 2 cut(s) 1115, 1139
BstNSI RCATGY 3 cut(s) 491, 1229, 1270
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 4 cut(s) 959, 1113, 1137, 1441
BstSFI CTRYAG 2 cut(s) 507, 1470
BstUI CGCG 2 cut(s) 107, 1023
BstV1I GCAGC 3 cut(s) 610, 1300, 1364
BstV2I GAAGAC 2 cut(s) 77, 408
BstX2I RGATCY 3 cut(s) 283, 856, 1044
BstXI CCANNNNNNTGG 2 cut(s) 692, 1386
BstYI RGATCY 3 cut(s) 283, 856, 1044
BsuRI GGCC 2 cut(s) 26, 1361
BtgI CCRYGG 3 cut(s) 189, 691, 1021
BtsCI GGATG 4 cut(s) 448, 969, 1129, 1471
BtsI GCAGTG 1 cut(s) 1404
BtsIMutI CAGTG 4 cut(s) 156, 573, 1161, 1404
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 7 cut(s) 255, 468, 843, 1191, 1268, 1286, 1363
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 109
Cfr42I CCGCGG 1 cut(s) 1024
CseI GACGC 3 cut(s) 113, 1207, 1376
Csp6I GTAC 1 cut(s) 994
CviAII CATG 9 cut(s) 190, 471, 488, 692, 1209, 1226, 1267, 1276, 1386
CviQI GTAC 1 cut(s) 994
DdeI CTNAG 8 cut(s) 148, 201, 555, 651, 918, 1040, 1085, 1164
DpnI GATC 4 cut(s) 285, 447, 858, 1046
DpnII GATC 4 cut(s) 283, 445, 856, 1044
DraI TTTAAA 1 cut(s) 1153
EaeI YGGCCR 2 cut(s) 24, 1359
Eam1104I CTCTTC 1 cut(s) 1332
EarI CTCTTC 1 cut(s) 1332
Eco130I CCWWGG 2 cut(s) 189, 691
Eco32I GATATC 2 cut(s) 264, 323
Eco57I CTGAAG 3 cut(s) 293, 561, 1510
Eco72I CACGTG 2 cut(s) 141, 815
EcoRI GAATTC 1 cut(s) 763
EcoRII CCWGG 2 cut(s) 1113, 1137
EcoRV GATATC 2 cut(s) 264, 323
EcoT14I CCWWGG 2 cut(s) 189, 691
ErhI CCWWGG 2 cut(s) 189, 691
FaeI CATG 9 cut(s) 193, 474, 491, 695, 1212, 1229, 1270, 1279, 1389
FalI AAGNNNNNCTT 2 cut(s) 1062, 1094
FaqI GGGAC 1 cut(s) 573
FatI CATG 9 cut(s) 189, 470, 487, 691, 1208, 1225, 1266, 1275, 1385
FauI CCCGC 2 cut(s) 418, 1277
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 8 cut(s) 27, 30, 195, 198, 624, 735, 1289, 1353
FokI GGATG 4 cut(s) 455, 976, 1136, 1478
Fsp4HI GCNGC 8 cut(s) 27, 30, 195, 198, 624, 735, 1289, 1353
GlaI GCGC 1 cut(s) 108
GluI GCNGC 8 cut(s) 27, 30, 195, 198, 624, 735, 1289, 1353
GsaI CCCAGC 1 cut(s) 1274
GsuI CTGGAG 3 cut(s) 74, 360, 1353
HaeIII GGCC 2 cut(s) 26, 1361
HapII CCGG 3 cut(s) 243, 961, 1443
HgaI GACGC 3 cut(s) 113, 1207, 1376
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 1368
Hin1II CATG 9 cut(s) 193, 474, 491, 695, 1212, 1229, 1270, 1279, 1389
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 3 cut(s) 464, 1076, 1407
HinfI GANTC 5 cut(s) 395, 503, 643, 1036, 1118
HpaII CCGG 3 cut(s) 243, 961, 1443
HphI GGTGA 3 cut(s) 225, 614, 667
Hpy166II GTNNAC 2 cut(s) 637, 996
Hpy188III TCNNGA 4 cut(s) 311, 419, 827, 929
Hpy8I GTNNAC 2 cut(s) 637, 996
Hpy99I CGWCG 2 cut(s) 107, 1370
HpyAV CCTTC 7 cut(s) 274, 453, 469, 613, 691, 1162, 1381
HpyCH4III ACNGT 3 cut(s) 217, 568, 1149
HpyCH4IV ACGT 5 cut(s) 140, 633, 814, 894, 1515
HpyF3I CTNAG 8 cut(s) 148, 201, 555, 651, 918, 1040, 1085, 1164
HpySE526I ACGT 5 cut(s) 140, 633, 814, 894, 1515
Hsp92I GRCGYC 1 cut(s) 1368
Hsp92II CATG 9 cut(s) 193, 474, 491, 695, 1212, 1229, 1270, 1279, 1389
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 445
KspI CCGCGG 1 cut(s) 1024
Kzo9I GATC 4 cut(s) 283, 445, 856, 1044
LmnI GCTCC 3 cut(s) 1177, 1281, 1449
Lsp1109I GCAGC 3 cut(s) 610, 1300, 1364
LweI GCATC 7 cut(s) 359, 715, 721, 888, 1186, 1384, 1456
MaeII ACGT 5 cut(s) 140, 633, 814, 894, 1515
MaeIII GTNAC 3 cut(s) 217, 1159, 1481
MalI GATC 4 cut(s) 285, 447, 858, 1046
MbiI CCGCTC 1 cut(s) 1284
MboI GATC 4 cut(s) 283, 445, 856, 1044
MboII GAAGA 7 cut(s) 77, 413, 554, 1336, 1349, 1366, 1415
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 3 cut(s) 283, 856, 1044
MlsI TGGCCA 1 cut(s) 1361
MluNI TGGCCA 1 cut(s) 1361
MlyI GAGTC 3 cut(s) 652, 1030, 1112
MmeI TCCRAC 2 cut(s) 508, 709
Mox20I TGGCCA 1 cut(s) 1361
MroXI GAANNNNTTC 3 cut(s) 121, 479, 1078
MscI TGGCCA 1 cut(s) 1361
MseI TTAA 7 cut(s) 183, 429, 563, 582, 849, 1152, 1310
MslI CAYNNNNRTG 3 cut(s) 375, 1213, 1384
Msp20I TGGCCA 1 cut(s) 1361
MspA1I CMGCKG 1 cut(s) 1023
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 3 cut(s) 243, 961, 1443
MspR9I CCNGG 4 cut(s) 961, 1115, 1139, 1443
MunI CAATTG 1 cut(s) 1242
Mva1269I GAATGC 2 cut(s) 626, 664
MvaI CCWGG 2 cut(s) 1115, 1139
MvnI CGCG 2 cut(s) 107, 1023
NciI CCSGG 2 cut(s) 961, 1443
NcoI CCATGG 2 cut(s) 189, 691
NdeII GATC 4 cut(s) 283, 445, 856, 1044
NlaIII CATG 9 cut(s) 193, 474, 491, 695, 1212, 1229, 1270, 1279, 1389
NlaIV GGNNCC 2 cut(s) 1046, 1173
NmuCI GTSAC 2 cut(s) 217, 1159
NspI RCATGY 3 cut(s) 491, 1229, 1270
PaeI GCATGC 1 cut(s) 1270
PaqCI CACCTGC 1 cut(s) 653
PciI ACATGT 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 2 cut(s) 626, 664
PdmI GAANNNNTTC 3 cut(s) 121, 479, 1078
PfeI GAWTC 2 cut(s) 395, 503
PkrI GCNGC 8 cut(s) 28, 31, 196, 199, 625, 736, 1290, 1354
PleI GAGTC 3 cut(s) 651, 1030, 1112
PmaCI CACGTG 2 cut(s) 141, 815
PmlI CACGTG 2 cut(s) 141, 815
PpsI GAGTC 3 cut(s) 651, 1030, 1112
Ppu21I YACGTR 2 cut(s) 141, 815
PscI ACATGT 1 cut(s) 487
PshAI GACNNNNGTC 1 cut(s) 642
Psp1406I AACGTT 1 cut(s) 1515
Psp6I CCWGG 2 cut(s) 1113, 1137
PspCI CACGTG 2 cut(s) 141, 815
PspFI CCCAGC 1 cut(s) 1270
PspGI CCWGG 2 cut(s) 1113, 1137
PspN4I GGNNCC 2 cut(s) 1046, 1173
PstI CTGCAG 1 cut(s) 511
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 3 cut(s) 283, 856, 1044
RsaI GTAC 1 cut(s) 995
RsaNI GTAC 1 cut(s) 994
RseI CAYNNNNRTG 3 cut(s) 375, 1213, 1384
SacII CCGCGG 1 cut(s) 1024
SaqAI TTAA 7 cut(s) 183, 429, 563, 582, 849, 1152, 1310
SatI GCNGC 8 cut(s) 27, 30, 195, 198, 624, 735, 1289, 1353
Sau3AI GATC 4 cut(s) 283, 445, 856, 1044
SchI GAGTC 3 cut(s) 652, 1030, 1112
ScrFI CCNGG 4 cut(s) 961, 1115, 1139, 1443
SfaNI GCATC 7 cut(s) 359, 715, 721, 888, 1186, 1384, 1456
SfcI CTRYAG 2 cut(s) 507, 1470
Sfr303I CCGCGG 1 cut(s) 1024
SgrBI CCGCGG 1 cut(s) 1024
SmiMI CAYNNNNRTG 3 cut(s) 375, 1213, 1384
SmlI CTYRAG 4 cut(s) 166, 419, 428, 1049
SmoI CTYRAG 4 cut(s) 166, 419, 428, 1049
SphI GCATGC 1 cut(s) 1270
StyD4I CCNGG 4 cut(s) 959, 1113, 1137, 1441
StyI CCWWGG 2 cut(s) 189, 691
TaaI ACNGT 3 cut(s) 217, 568, 1149
TaiI ACGT 5 cut(s) 143, 636, 817, 897, 1518
TaqI TCGA 2 cut(s) 335, 928
TatI WGTACW 1 cut(s) 993
TauI GCSGC 5 cut(s) 29, 32, 197, 200, 737
TfiI GAWTC 2 cut(s) 395, 503
Tru1I TTAA 7 cut(s) 183, 429, 563, 582, 849, 1152, 1310
Tru9I TTAA 7 cut(s) 183, 429, 563, 582, 849, 1152, 1310
TscAI CASTG 4 cut(s) 156, 573, 1168, 1404
TseFI GTSAC 2 cut(s) 217, 1159
TseI GCWGC 3 cut(s) 623, 1288, 1352
Tsp45I GTSAC 2 cut(s) 217, 1159
TspDTI ATGAA 1 cut(s) 1080
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 4 cut(s) 156, 573, 1168, 1404
Vha464I CTTAAG 1 cut(s) 428
XapI RAATTY 4 cut(s) 58, 548, 763, 924
XceI RCATGY 3 cut(s) 491, 1229, 1270
XmnI GAANNNNTTC 3 cut(s) 121, 479, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.