RLG00000030060

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002841
Physical Location & Seq
Forward (+)
54224113 .. 54229534
5422 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030060

Sequence Viewer

Length: 1797 bp
ATGCTTGGTTTTTCTGCTGCTGCTCGTGCGGCTACATCACCCAGCACCAATCTTGTCTTGCTATTCTCCACCAAGCTGCCAATTACTGATTCTTCTTCTTTCTCTTCTTTTACGTCTTGCTCACTGAAGAACTCATCTTGGCTGCACACCAGTTGCCGACCAATTAATGCCGTGGCTGCCCAAGAGGCTCAATCCTCAGCCTCAGGTGATAGGCAGGCTCTGATATCTTTGTCAGACAAAAACAATCTTGCTTTCTTAGGGAATGAGCTTCAGAACTTGGGATATAAAATTGTTTCCACTGGAGGAACTGCATCTGCTTTAGAAAGTGCCGGAGTGTCTGTCACTAAAGTTGAAGAACTTACTTGCTCCCAGGAGAAGCTCGATGGTCGTGTAAAAACTTTACACCCCAACATTCATGGGGGTATCCTTGCTAGAAGAGACCAAAAGCATCATATGGAAGCTCTTAGTAATCATGCAATTGGTACATTCGATGTGGTTGTGGTGAATTTATATCCCTTTTATGATAAAGTTACTTCAAAGGGAGGAATAGAATTTGAGGATGGAATCGAGAATATAGATATTGGTGGTCCTGCCATGATTAGAGCTGCTGCAAAGAATCACAAAGATGTCCTGGTGGTTGTTGATTCAGAAGACTATCATGCACTGCTAGAATATCTTAAAGCGGACAATGATGATCAACAATTCCGAAGAAACCTTGCGTGGAAGGCTTTTCAACATGTTGCTTCTTATGATTCTGCTGTTTCAGAGTGGCTTTGGAGGCAGACTACTGAAGTTACTTCTTTGAATAAATTTCCTCCTACCTTAACAGTTCCTCTATTGCTCAAAAGTCCCCTTCGGTATGGTGAAAATCCTCATCAAAAGGCTGCATTCTATGTTGACAAGAGTCTTTCTGAGGTTAATGCTGGTGGCTTTGCAACTGCTATTCAACATCATGGGAAGGAGATGTCATATAATAACTATTTAGATGCTGAAGCGGCTTGGAATTGTGTGTCAGAGTTCAGAAGTCCTACTTGTGTTGTTGTAAAACATACAAATCCTTGTGGTGTAGCATCACGTGATGATATACTTGACGCATACAGGCTTGCTGTCAAAGCAGATCCAGTGAGTGCATTTGGTGGCATTGTAGCTTTCAACATAGAGGTTGATGAGGCTCTTGCTAAAGAAATCCGTGAGTTTAGAAGCCCAACAGATGGTGAAACTCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTATTCAAAGAAAGGACTCGAAATCCTTCGTGTAAAATCAAAGACTCTGAGGATCCTCGAGGCAAGCAAAAATGAGAAAGGAAAACTTTCGCTTAAACAGGTTGGTGGTGGGTGGTTAGCCCAGGAGTCGGATGATTTAATTCCCCGAGATATCCAGTTTAATGTTGTGTCTAAGAAATCTCCAGAAGAAAGTGAGCTCAGTGATGCAGAGTTCGCATGGTTGTGTGTCAAACATGTCAAAAGCAATGCAATTGTAATAGCAAAGAATAACTGTATGTTGGGTATGGGAAGTGGACAGCCGAACCGTTTGGAGAGTTTAAGAATAGCATTGAGGAAAGCAGGAGAGGAGGTCAAGGGTGCAGCTTTGGCTAGCGATGCCTTCTTCCCATTCGCTTGGAAAGATGCCGTGGAAGAAGCAAGCGAAAGTGGAGTTAGTGTTATTGCAGAACCTGGTGGCAGTATAAGAGACGGAGATGCTATAGATTGCTGCAACAAGTATGGTGTTTCACTTGTTCTCACTAATGTGAGGCACTTCAGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

599

Amino Acids

65.28

Weight (kDa)

6.18

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGS PF02142 86 - 197 1.3e-19 MGS-like domain
AICARFT_IMPCHas PF01808 203 - 530 5.2e-113 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 1211
AciI CCGC 3 cut(s) 29, 683, 995
AclWI GGATC 3 cut(s) 1112, 1300, 1313
AcsI RAATTY 3 cut(s) 505, 551, 809
AcuI CTGAAG 5 cut(s) 146, 254, 810, 1011, 1771
AcvI CACGTG 1 cut(s) 1076
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 1211, 1381
AflIII ACRYGT 2 cut(s) 736, 1486
AgsI TTSAA 7 cut(s) 353, 537, 734, 805, 947, 1153, 1260
AjnI CCWGG 4 cut(s) 369, 630, 1374, 1702
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AleI CACNNNNGTG 1 cut(s) 1775
AluBI AGCT 8 cut(s) 76, 268, 379, 461, 605, 1148, 1450, 1616
AluI AGCT 8 cut(s) 76, 268, 379, 461, 605, 1148, 1450, 1616
Alw21I GWGCWC 1 cut(s) 1452
Alw26I GTCTC 2 cut(s) 432, 1713
AlwI GGATC 3 cut(s) 1112, 1300, 1313
AlwNI CAGNNNCTG 2 cut(s) 220, 1703
Ama87I CYCGRG 2 cut(s) 1310, 1398
ApoI RAATTY 3 cut(s) 505, 551, 809
AseI ATTAAT 1 cut(s) 165
Asp700I GAANNNNTTC 2 cut(s) 1278, 1339
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 5 cut(s) 30, 218, 514, 875, 1226
AsuNHI GCTAGC 1 cut(s) 1622
AvaI CYCGRG 2 cut(s) 1310, 1398
AvaII GGWCC 1 cut(s) 587
AxyI CCTNAGG 1 cut(s) 202
BamHI GGATCC 1 cut(s) 1305
BanII GRGCYC 1 cut(s) 1452
BauI CACGAG 1 cut(s) 24
BbrPI CACGTG 1 cut(s) 1076
BbsI GAAGAC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 1452
BbvCI CCTCAGC 1 cut(s) 196
BccI CCATC 3 cut(s) 377, 554, 1205
BceAI ACGGC 2 cut(s) 155, 1643
BciT130I CCWGG 4 cut(s) 371, 632, 1376, 1704
BciVI GTATCC 1 cut(s) 434
BclI TGATCA 1 cut(s) 694
BcoDI GTCTC 2 cut(s) 432, 1713
BfaI CTAG 3 cut(s) 432, 668, 1623
BfmI CTRYAG 1 cut(s) 1731
BfuI GTATCC 1 cut(s) 434
BglI GCCNNNNNGGC 1 cut(s) 185
Bme1390I CCNGG 4 cut(s) 371, 632, 1376, 1704
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 2 cut(s) 1310, 1398
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 1307
BmrFI CCNGG 4 cut(s) 371, 632, 1376, 1704
BmsI GCATC 8 cut(s) 320, 457, 976, 1079, 1447, 1618, 1645, 1717
BmtI GCTAGC 1 cut(s) 1626
BoxI GACNNNNGTC 1 cut(s) 903
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 2 cut(s) 321, 1419
Bpu10I CCTNAGC 1 cut(s) 196
BsaAI YACGTR 1 cut(s) 1076
BsaI GGTCTC 1 cut(s) 432
BsaJI CCNNGG 4 cut(s) 171, 369, 1374, 1659
BsaXI ACNNNNNCTCC 6 cut(s) 324, 354, 1587, 1617, 1674, 1704
Bsc4I CCNNNNNNNGG 2 cut(s) 1211, 1381
Bse1I ACTGG 4 cut(s) 150, 304, 1121, 1408
Bse21I CCTNAGG 1 cut(s) 202
Bse3DI GCAATG 1 cut(s) 1504
BseBI CCWGG 4 cut(s) 371, 632, 1376, 1704
BseDI CCNNGG 4 cut(s) 171, 369, 1374, 1659
BseGI GGATG 3 cut(s) 565, 1230, 1390
BseLI CCNNNNNNNGG 2 cut(s) 1211, 1381
BseMI GCAATG 1 cut(s) 1504
BseMII CTCAG 5 cut(s) 210, 216, 903, 1292, 1465
BseNI ACTGG 4 cut(s) 150, 304, 1121, 1408
BseRI GAGGAG 1 cut(s) 1613
BseYI CCCAGC 1 cut(s) 41
BsgI GTGCAG 2 cut(s) 128, 1632
BsiHKAI GWGCWC 1 cut(s) 1452
BsiHKCI CYCGRG 2 cut(s) 1310, 1398
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 2 cut(s) 1211, 1381
BsmAI GTCTC 2 cut(s) 432, 1713
BsmBI CGTCTC 1 cut(s) 1713
BsmFI GGGAC 1 cut(s) 834
BsmI GAATGC 1 cut(s) 887
Bso31I GGTCTC 1 cut(s) 432
BsoBI CYCGRG 2 cut(s) 1310, 1398
Bsp1286I GDGCHC 1 cut(s) 1452
Bsp143I GATC 3 cut(s) 694, 1117, 1305
BspACI CCGC 3 cut(s) 29, 683, 995
BspCNI CTCAG 5 cut(s) 209, 215, 904, 1293, 1464
BspLI GGNNCC 1 cut(s) 1307
BspOI GCTAGC 1 cut(s) 1626
BspPI GGATC 3 cut(s) 1112, 1300, 1313
BspTNI GGTCTC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 1504
BsrI ACTGG 4 cut(s) 150, 304, 1121, 1408
BssECI CCNNGG 4 cut(s) 171, 369, 1374, 1659
BssMI GATC 3 cut(s) 694, 1117, 1305
BssSI CACGAG 1 cut(s) 24
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 4 cut(s) 371, 632, 1376, 1704
Bst4CI ACNGT 3 cut(s) 829, 1526, 1559
Bst6I CTCTTC 2 cut(s) 109, 430
BstBAI YACGTR 1 cut(s) 1076
BstC8I GCNNGC 5 cut(s) 216, 1104, 1318, 1624, 1672
BstDEI CTNAG 8 cut(s) 196, 202, 256, 464, 912, 1301, 1425, 1451
BstDSI CCRYGG 2 cut(s) 171, 1659
BstF5I GGATG 3 cut(s) 565, 1230, 1390
BstKTI GATC 3 cut(s) 697, 1120, 1308
BstMAI GTCTC 2 cut(s) 432, 1713
BstMBI GATC 3 cut(s) 694, 1117, 1305
BstNI CCWGG 4 cut(s) 371, 632, 1376, 1704
BstNSI RCATGY 2 cut(s) 740, 1490
BstPAI GACNNNNGTC 1 cut(s) 903
BstSCI CCNGG 4 cut(s) 369, 630, 1374, 1702
BstSFI CTRYAG 1 cut(s) 1731
BstV2I GAAGAC 1 cut(s) 657
BstX2I RGATCY 2 cut(s) 1117, 1305
BstXI CCANNNNNNTGG 1 cut(s) 1647
BstYI RGATCY 2 cut(s) 1117, 1305
Bsu36I CCTNAGG 1 cut(s) 202
BsuI GTATCC 1 cut(s) 434
BtgI CCRYGG 2 cut(s) 171, 1659
BtgZI GCGATG 1 cut(s) 1641
BtsCI GGATG 3 cut(s) 565, 1230, 1390
BtsI GCAGTG 1 cut(s) 662
BtsIMutI CAGTG 5 cut(s) 122, 297, 662, 1128, 1459
Cac8I GCNNGC 5 cut(s) 216, 1104, 1318, 1624, 1672
CaiI CAGNNNCTG 2 cut(s) 220, 1703
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 1 cut(s) 1100
CsiI ACCWGGT 1 cut(s) 1702
Csp6I GTAC 1 cut(s) 483
CviAII CATG 8 cut(s) 416, 473, 595, 659, 737, 953, 1470, 1487
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 8 cut(s) 196, 202, 256, 464, 912, 1301, 1425, 1451
DpnI GATC 3 cut(s) 696, 1119, 1307
DpnII GATC 3 cut(s) 694, 1117, 1305
Eam1104I CTCTTC 2 cut(s) 109, 430
EarI CTCTTC 2 cut(s) 109, 430
Ecl136II GAGCTC 1 cut(s) 1450
Eco24I GRGCYC 1 cut(s) 1452
Eco31I GGTCTC 1 cut(s) 432
Eco32I GATATC 2 cut(s) 225, 1405
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 1450
Eco57I CTGAAG 5 cut(s) 146, 254, 810, 1011, 1771
Eco72I CACGTG 1 cut(s) 1076
Eco81I CCTNAGG 1 cut(s) 202
Eco88I CYCGRG 2 cut(s) 1310, 1398
EcoICRI GAGCTC 1 cut(s) 1450
EcoRII CCWGG 4 cut(s) 369, 630, 1374, 1702
EcoRV GATATC 2 cut(s) 225, 1405
EcoT38I GRGCYC 1 cut(s) 1452
Esp3I CGTCTC 1 cut(s) 1713
FaeI CATG 8 cut(s) 419, 476, 598, 662, 740, 956, 1473, 1490
FalI AAGNNNNNCTT 4 cut(s) 1015, 1047, 1323, 1355
FaqI GGGAC 1 cut(s) 834
FatI CATG 8 cut(s) 415, 472, 594, 658, 736, 952, 1469, 1486
FauNDI CATATG 1 cut(s) 453
FbaI TGATCA 1 cut(s) 694
FokI GGATG 3 cut(s) 572, 1237, 1397
FriOI GRGCYC 1 cut(s) 1452
FspBI CTAG 3 cut(s) 432, 668, 1623
GsaI CCCAGC 1 cut(s) 45
GsuI CTGGAG 2 cut(s) 321, 1419
HapII CCGG 1 cut(s) 330
HgaI GACGC 1 cut(s) 1100
Hin1II CATG 8 cut(s) 419, 476, 598, 662, 740, 956, 1473, 1490
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HinfI GANTC 9 cut(s) 89, 564, 616, 644, 752, 904, 1269, 1297, 1379
HpaII CCGG 1 cut(s) 330
HphI GGTGA 5 cut(s) 30, 218, 514, 875, 1226
Hpy166II GTNNAC 2 cut(s) 898, 1547
Hpy188III TCNNGA 2 cut(s) 568, 1436
Hpy8I GTNNAC 2 cut(s) 898, 1547
HpyAV CCTTC 5 cut(s) 718, 863, 952, 1289, 1642
HpyCH4III ACNGT 3 cut(s) 829, 1526, 1559
HpyCH4IV ACGT 2 cut(s) 113, 1075
HpyF3I CTNAG 8 cut(s) 196, 202, 256, 464, 912, 1301, 1425, 1451
HpySE526I ACGT 2 cut(s) 113, 1075
Hsp92II CATG 8 cut(s) 419, 476, 598, 662, 740, 956, 1473, 1490
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 3 cut(s) 694, 1117, 1305
LmnI GCTCC 1 cut(s) 371
LweI GCATC 8 cut(s) 320, 457, 976, 1079, 1447, 1618, 1645, 1717
MabI ACCWGGT 1 cut(s) 1702
MaeI CTAG 3 cut(s) 432, 668, 1623
MaeII ACGT 2 cut(s) 113, 1075
MaeIII GTNAC 3 cut(s) 340, 529, 793
MalI GATC 3 cut(s) 696, 1119, 1307
MboI GATC 3 cut(s) 694, 1117, 1305
MfeI CAATTG 2 cut(s) 477, 1503
MflI RGATCY 2 cut(s) 1117, 1305
MhlI GDGCHC 1 cut(s) 1452
MlyI GAGTC 4 cut(s) 913, 1263, 1291, 1388
MmeI TCCRAC 1 cut(s) 1362
MroXI GAANNNNTTC 2 cut(s) 1278, 1339
MseI TTAA 9 cut(s) 165, 678, 824, 918, 1347, 1391, 1413, 1571, 1795
MslI CAYNNNNRTG 3 cut(s) 624, 1474, 1775
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 4 cut(s) 371, 632, 1376, 1704
MunI CAATTG 2 cut(s) 477, 1503
Mva1269I GAATGC 1 cut(s) 887
MvaI CCWGG 4 cut(s) 371, 632, 1376, 1704
NdeI CATATG 1 cut(s) 453
NdeII GATC 3 cut(s) 694, 1117, 1305
NheI GCTAGC 1 cut(s) 1622
NlaIII CATG 8 cut(s) 419, 476, 598, 662, 740, 956, 1473, 1490
NlaIV GGNNCC 1 cut(s) 1307
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 2 cut(s) 740, 1490
OliI CACNNNNGTG 1 cut(s) 1775
PaeR7I CTCGAG 1 cut(s) 1310
PciI ACATGT 2 cut(s) 736, 1486
PctI GAATGC 1 cut(s) 887
PdmI GAANNNNTTC 2 cut(s) 1278, 1339
PfeI GAWTC 5 cut(s) 89, 564, 616, 644, 752
PflMI CCANNNNNTGG 1 cut(s) 1211
PleI GAGTC 4 cut(s) 912, 1263, 1291, 1387
PmaCI CACGTG 1 cut(s) 1076
PmlI CACGTG 1 cut(s) 1076
PpsI GAGTC 4 cut(s) 912, 1263, 1291, 1387
Ppu21I YACGTR 1 cut(s) 1076
PscI ACATGT 2 cut(s) 736, 1486
PshAI GACNNNNGTC 1 cut(s) 903
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 1452
Psp6I CCWGG 4 cut(s) 369, 630, 1374, 1702
PspCI CACGTG 1 cut(s) 1076
PspFI CCCAGC 1 cut(s) 41
PspGI CCWGG 4 cut(s) 369, 630, 1374, 1702
PspN4I GGNNCC 1 cut(s) 1307
PspPI GGNCC 1 cut(s) 587
PspXI VCTCGAGB 1 cut(s) 1310
PstNI CAGNNNCTG 2 cut(s) 220, 1703
PsuI RGATCY 2 cut(s) 1117, 1305
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
RseI CAYNNNNRTG 3 cut(s) 624, 1474, 1775
SacI GAGCTC 1 cut(s) 1452
SaqAI TTAA 9 cut(s) 165, 678, 824, 918, 1347, 1391, 1413, 1571, 1795
Sau3AI GATC 3 cut(s) 694, 1117, 1305
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 4 cut(s) 913, 1263, 1291, 1388
ScrFI CCNGG 4 cut(s) 371, 632, 1376, 1704
SduI GDGCHC 1 cut(s) 1452
SexAI ACCWGGT 1 cut(s) 1702
SfaNI GCATC 8 cut(s) 320, 457, 976, 1079, 1447, 1618, 1645, 1717
SfcI CTRYAG 1 cut(s) 1731
Sfr274I CTCGAG 1 cut(s) 1310
SinI GGWCC 1 cut(s) 587
SlaI CTCGAG 1 cut(s) 1310
SmiMI CAYNNNNRTG 3 cut(s) 624, 1474, 1775
SmlI CTYRAG 1 cut(s) 1310
SmoI CTYRAG 1 cut(s) 1310
SsiI CCGC 3 cut(s) 29, 683, 995
SspMI CTAG 3 cut(s) 432, 668, 1623
SstI GAGCTC 1 cut(s) 1452
StyD4I CCNGG 4 cut(s) 369, 630, 1374, 1702
TaaI ACNGT 3 cut(s) 829, 1526, 1559
TaiI ACGT 2 cut(s) 116, 1078
TaqI TCGA 5 cut(s) 381, 489, 567, 1272, 1311
TauI GCSGC 2 cut(s) 32, 998
TfiI GAWTC 5 cut(s) 89, 564, 616, 644, 752
Tru1I TTAA 9 cut(s) 165, 678, 824, 918, 1347, 1391, 1413, 1571, 1795
Tru9I TTAA 9 cut(s) 165, 678, 824, 918, 1347, 1391, 1413, 1571, 1795
TscAI CASTG 5 cut(s) 129, 304, 669, 1128, 1459
TseFI GTSAC 1 cut(s) 340
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 1178, 1737
TspRI CASTG 5 cut(s) 129, 304, 669, 1128, 1459
Van91I CCANNNNNTGG 1 cut(s) 1211
VpaK11BI GGWCC 1 cut(s) 587
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 3 cut(s) 505, 551, 809
XceI RCATGY 2 cut(s) 740, 1490
XhoI CTCGAG 1 cut(s) 1310
XmnI GAANNNNTTC 2 cut(s) 1278, 1339
XspI CTAG 3 cut(s) 432, 668, 1623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.