RchiOBHm_Chr2g0124681

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002841
Physical Location & Seq
Forward (+)
37977138 .. 37983240
6103 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49684

Sequence Viewer

Length: 1791 bp
ATGCTTGGTTTGTCCGCTGCTGCTCCTGCGGCCATAACACCCAACACCAATCTTGTCCTGCTTTTCTCCACCAAACTTCCAATTACTCATTCGTCTTCTTTCTCTTCTTTCACCGCGTCTTGTTCTCTCAGGAACTCATCTCGCACTTGTACTCCAATCAAAGCCATGGCTGCCCAAGAGCCTCAATCCCCTGCCTCAGCCTCGGCCTCAGGTGATAGGCAGGCTCTGATATCTTTGTCAGACAAAAGCAATCTTGCTTTCTTAGGCAACGGGCTTCAGAACTTGGGATATAAAATTGTTTCCACTGGAGGAACTGCATCTGCTTTAGAAAGTGCCGGGGTGTCTGTTACAAAAGTTGAAGAAATTACTTGCTTCCCGGAGATGCTCGATGGTCGTGTAAAAACTTTACACCCCAACATTCATGGGGGTATCCTTGCTAGAAGAGATCAAAAACATCATGTGGAAGCTCTTAATAATCACGGAATTGGTACATTCGATGTGGTTGTGGTGAATTTATATCCCTTTTATGATAAAGTTACTTCAAAGGGTGGAATAGAATTTGAGGATGGAATCGAGAATATAGATATTGGTGGTCCTGCCATGATTAGAGCTGCTGCAAAGAATCACAAAGATGTCCTGGTGGTTGTTGATTCAGAAGACTATCCTGCACTGCTAGAATATCTTAAAGCAGACAAGGATGATCAACAATTCCGAAGAAACCTTGCGTGGAAGGCTTTTCAACATGTTGCTTCTTATGATTCTGCTGTTTCAGAGTGGCTGTGGAGGCAGACTACCGAAGATAAATTCCCGCCTAGCTTAACAGTTCCTCTATTGCTCAAAAGTCCCCTTCGGTATGGTGAAAATCCTCATCAAAGGGCTGCATTTTATGTTGATAAGAGTCTTTCTGAGGTTAATGCTGGTGGCATTGCAACTGCTATCCAACATCATGGGAAGGAGATGTCATATAATAACTATTTAGATGCTGATGCGGCTTGGAATTGTGTGTCAGAGTTCAGAAGTCCTACCTGTGTTGTTGTAAAGCATACAAATCCTTGTGGTGTAGCATCACGTGATGATATACTTGAAGCATACAGGCTTGCTGTCAAAGCAGATCCAGTGAGTGCATTTGGTGGCATTGTAGCTTTCAACATAGAGGTTGATGAGGCTCTTGCTAAAGAAATCCGAGAGTTTAGAAGCCCAACAGATGGTGAAACTCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTATTCAAAGAAAGGTCTTGAAATTCTTCGTGGAAAATCAAAGACTCTGAGGATCCTCGAGGCAAGTAAAAATGAGAAAGGAAAACTTTCGCTTAGACAGGTTGGTGGTGGGTGGTTAGCCCAGGACTCGGATGATTTAAGTCCCCAAGATATCCAGTTTAATGTTGTGTCTAAGAAGTCTCCACAAGAAAGTGAGCTCAGTGATGCAGAGTTTGCATGGTTGTGTGTCAAACATGTCAAAAGCAATGCAATTGTAATAGCAAAGAATAACTGTATGTTGGGTATGGGAAGTGGACAGCCGAACCGTCGGGAGAGTTTAAGAATAGCACTGAGGAAAGCAGGAGAGGAGGTCAAGGGTGCAGCTTTGGCTAGCGATGCCTTCTTCCCATTCGCTTGGAAAGATGCCGTGGAAGAAGCATGTGAAAGTGGAGTAAGTGTTATTGCAGAACCTGGTGGCAGTATAAGAGATGGAGATGCTATAGATTGCTGCAACAAGTATGGTGTTTCTCTTGTTTTCACTAATGTGAGGCACTTCAGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

596

Amino Acids

64.81

Weight (kDa)

6.34

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGS PF02142 88 - 199 1.7e-20 MGS-like domain
AICARFT_IMPCHas PF01808 205 - 528 7.9e-114 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1304
AccB7I CCANNNNNTGG 1 cut(s) 1205
AccII CGCG 1 cut(s) 116
AciI CCGC 5 cut(s) 15, 29, 114, 809, 989
AclWI GGATC 3 cut(s) 1106, 1294, 1307
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 4 cut(s) 511, 557, 803, 1269
AcuI CTGAAG 2 cut(s) 260, 1765
AcvI CACGTG 1 cut(s) 1070
AfaI GTAC 2 cut(s) 151, 490
AfiI CCNNNNNNNGG 2 cut(s) 1205, 1375
AflIII ACRYGT 2 cut(s) 742, 1480
AgsI TTSAA 7 cut(s) 359, 543, 740, 1085, 1147, 1254, 1268
AjnI CCWGG 3 cut(s) 636, 1368, 1696
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AleI CACNNNNGTG 1 cut(s) 1769
AluBI AGCT 6 cut(s) 467, 611, 816, 1142, 1444, 1610
AluI AGCT 6 cut(s) 467, 611, 816, 1142, 1444, 1610
Alw21I GWGCWC 1 cut(s) 1446
Alw26I GTCTC 1 cut(s) 1431
AlwI GGATC 3 cut(s) 1106, 1294, 1307
AlwNI CAGNNNCTG 2 cut(s) 226, 1697
Ama87I CYCGRG 1 cut(s) 1304
AoxI GGCC 2 cut(s) 30, 204
ApeKI GCWGC 8 cut(s) 17, 20, 170, 611, 614, 878, 1607, 1734
ApoI RAATTY 4 cut(s) 511, 557, 803, 1269
Asp700I GAANNNNTTC 2 cut(s) 1272, 1333
AspS9I GGNCC 1 cut(s) 593
AsuC2I CCSGG 2 cut(s) 337, 377
AsuHPI GGTGA 5 cut(s) 103, 224, 520, 869, 1220
AsuNHI GCTAGC 1 cut(s) 1616
AvaI CYCGRG 1 cut(s) 1304
AvaII GGWCC 1 cut(s) 593
AxyI CCTNAGG 1 cut(s) 208
BamHI GGATCC 1 cut(s) 1299
BanII GRGCYC 1 cut(s) 1446
BbrPI CACGTG 1 cut(s) 1070
BbsI GAAGAC 2 cut(s) 87, 663
Bbv12I GWGCWC 1 cut(s) 1446
BbvCI CCTCAGC 1 cut(s) 196
BbvI GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
BccI CCATC 4 cut(s) 383, 560, 1199, 1709
BceAI ACGGC 1 cut(s) 1637
BciT130I CCWGG 3 cut(s) 638, 1370, 1698
BciVI GTATCC 1 cut(s) 440
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 2 cut(s) 337, 377
BcoDI GTCTC 1 cut(s) 1431
BfaI CTAG 4 cut(s) 438, 674, 813, 1617
BfmI CTRYAG 1 cut(s) 1725
BfuI GTATCC 1 cut(s) 440
Bme1390I CCNGG 5 cut(s) 337, 377, 638, 1370, 1698
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 1304
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 1301
BmrFI CCNGG 5 cut(s) 337, 377, 638, 1370, 1698
BmsI GCATC 9 cut(s) 326, 372, 970, 976, 1073, 1441, 1612, 1639, 1711
BmtI GCTAGC 1 cut(s) 1620
BpiI GAAGAC 2 cut(s) 87, 663
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 196
BpuMI CCSGG 2 cut(s) 337, 377
BsaAI YACGTR 1 cut(s) 1070
BsaJI CCNNGG 5 cut(s) 165, 201, 336, 1368, 1653
BsaXI ACNNNNNCTCC 6 cut(s) 136, 166, 1581, 1611, 1668, 1698
Bsc4I CCNNNNNNNGG 2 cut(s) 1205, 1375
Bse1I ACTGG 3 cut(s) 310, 1115, 1402
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 2 cut(s) 924, 1498
BseBI CCWGG 3 cut(s) 638, 1370, 1698
BseDI CCNNGG 5 cut(s) 165, 201, 336, 1368, 1653
BseGI GGATG 4 cut(s) 571, 703, 1224, 1384
BseLI CCNNNNNNNGG 2 cut(s) 1205, 1375
BseMI GCAATG 2 cut(s) 924, 1498
BseMII CTCAG 7 cut(s) 142, 210, 222, 897, 1286, 1459, 1568
BseNI ACTGG 3 cut(s) 310, 1115, 1402
BseRI GAGGAG 1 cut(s) 1607
BseXI GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
BsgI GTGCAG 2 cut(s) 651, 1626
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 2 cut(s) 32, 206
BsiHKAI GWGCWC 1 cut(s) 1446
BsiHKCI CYCGRG 1 cut(s) 1304
BsiSI CCGG 2 cut(s) 336, 377
BslFI GGGAC 2 cut(s) 828, 1374
BslI CCNNNNNNNGG 2 cut(s) 1205, 1375
BsmAI GTCTC 1 cut(s) 1431
BsmFI GGGAC 2 cut(s) 828, 1374
BsnI GGCC 2 cut(s) 32, 206
BsoBI CYCGRG 1 cut(s) 1304
Bsp1286I GDGCHC 1 cut(s) 1446
Bsp143I GATC 4 cut(s) 445, 700, 1111, 1299
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 5 cut(s) 15, 29, 114, 809, 989
BspANI GGCC 2 cut(s) 32, 206
BspCNI CTCAG 7 cut(s) 141, 209, 221, 898, 1287, 1458, 1569
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 1301
BspOI GCTAGC 1 cut(s) 1620
BspPI GGATC 3 cut(s) 1106, 1294, 1307
BsrDI GCAATG 2 cut(s) 924, 1498
BsrI ACTGG 3 cut(s) 310, 1115, 1402
BssECI CCNNGG 5 cut(s) 165, 201, 336, 1368, 1653
BssMI GATC 4 cut(s) 445, 700, 1111, 1299
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 638, 1370, 1698
Bst4CI ACNGT 3 cut(s) 823, 1520, 1553
Bst6I CTCTTC 2 cut(s) 109, 436
BstAPI GCANNNNNTGC 1 cut(s) 1460
BstBAI YACGTR 1 cut(s) 1070
BstC8I GCNNGC 3 cut(s) 222, 1098, 1618
BstDSI CCRYGG 2 cut(s) 165, 1653
BstF5I GGATG 4 cut(s) 571, 703, 1224, 1384
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 4 cut(s) 448, 703, 1114, 1302
BstMAI GTCTC 1 cut(s) 1431
BstMBI GATC 4 cut(s) 445, 700, 1111, 1299
BstNI CCWGG 3 cut(s) 638, 1370, 1698
BstNSI RCATGY 3 cut(s) 746, 1484, 1668
BstSCI CCNGG 5 cut(s) 335, 375, 636, 1368, 1696
BstSFI CTRYAG 1 cut(s) 1725
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
BstV2I GAAGAC 2 cut(s) 87, 663
BstX2I RGATCY 2 cut(s) 1111, 1299
BstXI CCANNNNNNTGG 2 cut(s) 947, 1641
BstYI RGATCY 2 cut(s) 1111, 1299
Bsu36I CCTNAGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 2 cut(s) 32, 206
BtgI CCRYGG 2 cut(s) 165, 1653
BtgZI GCGATG 1 cut(s) 1635
BtsCI GGATG 4 cut(s) 571, 703, 1224, 1384
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 5 cut(s) 303, 668, 1122, 1453, 1574
Cac8I GCNNGC 3 cut(s) 222, 1098, 1618
CaiI CAGNNNCTG 2 cut(s) 226, 1697
Cfr13I GGNCC 1 cut(s) 593
CseI GACGC 1 cut(s) 105
CsiI ACCWGGT 1 cut(s) 1696
Csp6I GTAC 2 cut(s) 150, 489
CviAII CATG 9 cut(s) 166, 422, 458, 601, 743, 947, 1464, 1481, 1665
CviQI GTAC 2 cut(s) 150, 489
DpnI GATC 4 cut(s) 447, 702, 1113, 1301
DpnII GATC 4 cut(s) 445, 700, 1111, 1299
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 109, 436
EarI CTCTTC 2 cut(s) 109, 436
Ecl136II GAGCTC 1 cut(s) 1444
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 1446
Eco32I GATATC 2 cut(s) 231, 1399
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 2 cut(s) 260, 1765
Eco72I CACGTG 1 cut(s) 1070
Eco81I CCTNAGG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 1304
EcoICRI GAGCTC 1 cut(s) 1444
EcoRII CCWGG 3 cut(s) 636, 1368, 1696
EcoRV GATATC 2 cut(s) 231, 1399
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 1446
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 9 cut(s) 169, 425, 461, 604, 746, 950, 1467, 1484, 1668
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FaqI GGGAC 2 cut(s) 828, 1374
FatI CATG 9 cut(s) 165, 421, 457, 600, 742, 946, 1463, 1480, 1664
FauI CCCGC 1 cut(s) 816
FbaI TGATCA 1 cut(s) 700
FokI GGATG 4 cut(s) 578, 710, 1231, 1391
FriOI GRGCYC 1 cut(s) 1446
FspBI CTAG 4 cut(s) 438, 674, 813, 1617
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 2 cut(s) 32, 206
HapII CCGG 2 cut(s) 336, 377
HgaI GACGC 1 cut(s) 105
Hin1II CATG 9 cut(s) 169, 425, 461, 604, 746, 950, 1467, 1484, 1668
HinfI GANTC 7 cut(s) 570, 622, 650, 758, 898, 1291, 1373
HpaII CCGG 2 cut(s) 336, 377
HphI GGTGA 5 cut(s) 103, 224, 520, 869, 1220
Hpy166II GTNNAC 1 cut(s) 1541
Hpy188III TCNNGA 4 cut(s) 130, 574, 1265, 1556
Hpy8I GTNNAC 1 cut(s) 1541
Hpy99I CGWCG 1 cut(s) 1557
HpyAV CCTTC 4 cut(s) 724, 857, 946, 1636
HpyCH4III ACNGT 3 cut(s) 823, 1520, 1553
HpyCH4IV ACGT 1 cut(s) 1069
HpySE526I ACGT 1 cut(s) 1069
Hsp92II CATG 9 cut(s) 169, 425, 461, 604, 746, 950, 1467, 1484, 1668
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 4 cut(s) 445, 700, 1111, 1299
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
LweI GCATC 9 cut(s) 326, 372, 970, 976, 1073, 1441, 1612, 1639, 1711
MabI ACCWGGT 1 cut(s) 1696
MaeI CTAG 4 cut(s) 438, 674, 813, 1617
MaeII ACGT 1 cut(s) 1069
MaeIII GTNAC 2 cut(s) 346, 535
MalI GATC 4 cut(s) 447, 702, 1113, 1301
MboI GATC 4 cut(s) 445, 700, 1111, 1299
MfeI CAATTG 1 cut(s) 1497
MflI RGATCY 2 cut(s) 1111, 1299
MhlI GDGCHC 1 cut(s) 1446
MlyI GAGTC 3 cut(s) 907, 1285, 1367
MmeI TCCRAC 1 cut(s) 964
MroXI GAANNNNTTC 2 cut(s) 1272, 1333
MseI TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
MslI CAYNNNNRTG 3 cut(s) 630, 1468, 1769
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 336, 377
MspR9I CCNGG 5 cut(s) 337, 377, 638, 1370, 1698
MunI CAATTG 1 cut(s) 1497
MvaI CCWGG 3 cut(s) 638, 1370, 1698
MvnI CGCG 1 cut(s) 116
NciI CCSGG 2 cut(s) 337, 377
NcoI CCATGG 1 cut(s) 165
NdeII GATC 4 cut(s) 445, 700, 1111, 1299
NheI GCTAGC 1 cut(s) 1616
NlaIII CATG 9 cut(s) 169, 425, 461, 604, 746, 950, 1467, 1484, 1668
NlaIV GGNNCC 1 cut(s) 1301
NmeAIII GCCGAG 1 cut(s) 182
NspI RCATGY 3 cut(s) 746, 1484, 1668
OliI CACNNNNGTG 1 cut(s) 1769
PaeR7I CTCGAG 1 cut(s) 1304
PciI ACATGT 2 cut(s) 742, 1480
PdmI GAANNNNTTC 2 cut(s) 1272, 1333
PfeI GAWTC 4 cut(s) 570, 622, 650, 758
PflMI CCANNNNNTGG 1 cut(s) 1205
PfoI TCCNGGA 1 cut(s) 375
PleI GAGTC 3 cut(s) 906, 1285, 1367
PmaCI CACGTG 1 cut(s) 1070
PmlI CACGTG 1 cut(s) 1070
PpsI GAGTC 3 cut(s) 906, 1285, 1367
Ppu21I YACGTR 1 cut(s) 1070
PscI ACATGT 2 cut(s) 742, 1480
Psp124BI GAGCTC 1 cut(s) 1446
Psp6I CCWGG 3 cut(s) 636, 1368, 1696
PspCI CACGTG 1 cut(s) 1070
PspGI CCWGG 3 cut(s) 636, 1368, 1696
PspN4I GGNNCC 1 cut(s) 1301
PspPI GGNCC 1 cut(s) 593
PspXI VCTCGAGB 1 cut(s) 1304
PstNI CAGNNNCTG 2 cut(s) 226, 1697
PsuI RGATCY 2 cut(s) 1111, 1299
RsaI GTAC 2 cut(s) 151, 490
RsaNI GTAC 2 cut(s) 150, 489
RseI CAYNNNNRTG 3 cut(s) 630, 1468, 1769
SacI GAGCTC 1 cut(s) 1446
SaqAI TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
Sau3AI GATC 4 cut(s) 445, 700, 1111, 1299
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 3 cut(s) 907, 1285, 1367
ScrFI CCNGG 5 cut(s) 337, 377, 638, 1370, 1698
SduI GDGCHC 1 cut(s) 1446
SexAI ACCWGGT 1 cut(s) 1696
SfaNI GCATC 9 cut(s) 326, 372, 970, 976, 1073, 1441, 1612, 1639, 1711
SfcI CTRYAG 1 cut(s) 1725
Sfr274I CTCGAG 1 cut(s) 1304
SinI GGWCC 1 cut(s) 593
SlaI CTCGAG 1 cut(s) 1304
SmiMI CAYNNNNRTG 3 cut(s) 630, 1468, 1769
SmlI CTYRAG 1 cut(s) 1304
SmoI CTYRAG 1 cut(s) 1304
SsiI CCGC 5 cut(s) 15, 29, 114, 809, 989
SspMI CTAG 4 cut(s) 438, 674, 813, 1617
SstI GAGCTC 1 cut(s) 1446
StyD4I CCNGG 5 cut(s) 335, 375, 636, 1368, 1696
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 3 cut(s) 823, 1520, 1553
TaiI ACGT 1 cut(s) 1072
TaqI TCGA 4 cut(s) 387, 495, 573, 1305
TatI WGTACW 1 cut(s) 149
TauI GCSGC 2 cut(s) 32, 992
TfiI GAWTC 4 cut(s) 570, 622, 650, 758
Tru1I TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
Tru9I TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
TscAI CASTG 5 cut(s) 310, 675, 1122, 1453, 1581
TseI GCWGC 8 cut(s) 17, 20, 170, 611, 614, 878, 1607, 1734
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 5 cut(s) 310, 675, 1122, 1453, 1581
Van91I CCANNNNNTGG 1 cut(s) 1205
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 4 cut(s) 511, 557, 803, 1269
XceI RCATGY 3 cut(s) 746, 1484, 1668
XhoI CTCGAG 1 cut(s) 1304
XmnI GAANNNNTTC 2 cut(s) 1272, 1333
XspI CTAG 4 cut(s) 438, 674, 813, 1617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.