FvH4_1g24580

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002841
Physical Location & Seq
Reverse (-)
16408367 .. 16414307
5941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24580.t1

Sequence Viewer

Length: 1794 bp
ATGCTGCTTCATTTGTCTGCTGCCCCTCCTGCCAGCACGACACCCAACACCAGTCTTTTCCTGCTCTTCTCCACCAATCTTCTGATTACTCATTCTTCGTCTTCTTTCTCGCCTTGTTCACTCAGGAACTCCTCTGGGCTGCACACCAGTTGTAGACCACTTAAGGCCATGGCTGCCCAAGAGGCTCAATTCTCAACCTCAGCCTCAGCCTCAGGTGATAGGCAGGCTCTGATATCCTTGTCAGACAAAAACAATCTTGCCTTCCTAGGGAATGGGCTTCAGAGCTTGGGATATAAAATTGTTTCCACGGGAGGAACTGCATCTGCTTTGGAAAGTGCCGGGGTGTCTGTCACTAAAGTTGAAGAACTTACTTGCTTCCCGGAGATGCTCGATGGTCGTGTTAAAACTTTACACCCCAACATTCATGGTGGTATTCTTGCTAGAAGAGACCAAAAGCATCACATGGAAGCTCTTAGTAATCACGGAATTGGTATATTTGATGTGGTTGTGGTGAATCTATATCCCTTTTATGATAAAGTTACTTCAACTGGAGGAATAGAATTTGAGGATGGAATCGAAAATATAGACATTGGTGGTCCTGCCATGATTAGAGCTGCTGCAAAGAATCACAAAGATGTCCTGGTGGTTGTTGATTCAGAAGACTATCCTACACTTTTAGAATATCTTAAAGTGGACAAGGATGATCAACAATTCCGAAGAAAGCTTGCGTGGAAGGCTTTCCAACATGTTGCTTCTTATGATTCTGCTGTTTCAGAGTGGCTTTGGAGGCAGACTAGCGAAGATAAATTCCCCCCAAGCTTAACAGTTCCTCTTTCGCTCAAAAGTCCCCTTCGCTATGGTGAAAATCCTCATCAAAAGGCTGCATTTTATGTTGACAAGAGTCTTTCTGAGGTTAATGCTGGTGGCATTGCAACTGCTATACAACATCATGGGAAGGAGATGTCATATAATAACTATTTAGATGCTGATGCGGCTTGGAATTGTGTGTCAGAGTTCAGGGGTCCTACCTGTGTTGTTGTAAAGCATACAAATCCTTGCGGTGTAGCATCACGTGATGACATACTTGAAGCATACAGGCTTGCTGTCAAAGCAGATCCAGTGAGTGCATTTGGTGGCATTGTAGCTTTCAACATAGAGGTTGATGAGGCTCTTGCTAAAGAAATCCGAGAGTTTAGAAGCCCAACAGATGGTGAAACCCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTATTCAAAGAAAGGTCTTGAGATACTTCGTGGAAAATCAAAGACTCTGAGGATCCTTGAGGCAAGCAAAAATGAGAAAGGAAAACTTTCACTGAGACAGGTTGGTGGTGGGTGGTTAGCCCAGGACTCAGACGATTTAACTCCTCAAGATATCCAATTTAATGTTGTGTCTAAGAAGTCTCCACAAGAAAGTGAGCTCAGTGATGCAGAGTTTGCATGGTTGTGTGTCAAACATGTCAAGAGCAATGCAATTGTACTAGCAAAGAATAACTGTATGTTGGGTATGGGAAGTGGACAGCCGAATCGTCGGGAGAGTTTAAGAATAGCATTGAAGAAAGCAGGAGAGGAGGTCAAGGGTGCATCTTTGGCTAGTGATGCCTTCTTTCCATTCGCTTGGAAAGATGCCGTGGAAGAAGCATGTGAAAGTGGAGTTAGTGTTATTGCAGAACCTGGTGGGAGTATAAGAGACGGAGATGCTATAGATTGCTGCAATAAGTATGGCGTTTCACTTGTTTTCACTAACATGAGGCACTTCAGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

598

Amino Acids

65.1

Weight (kDa)

6.3

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGS PF02142 90 - 200 2.2e-20 MGS-like domain
AICARFT_IMPCHas PF01808 206 - 529 2.3e-114 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1208
AccI GTMKAC 1 cut(s) 154
AciI CCGC 2 cut(s) 992, 1059
AclWI GGATC 3 cut(s) 1109, 1297, 1310
AcsI RAATTY 2 cut(s) 560, 806
AcuI CTGAAG 2 cut(s) 263, 1768
AcvI CACGTG 1 cut(s) 1073
AfaI GTAC 1 cut(s) 1506
AfiI CCNNNNNNNGG 2 cut(s) 267, 1208
AflII CTTAAG 1 cut(s) 161
AflIII ACRYGT 2 cut(s) 745, 1483
AgsI TTSAA 6 cut(s) 362, 546, 1088, 1150, 1257, 1582
AjnI CCWGG 3 cut(s) 639, 1371, 1699
AluBI AGCT 7 cut(s) 285, 470, 614, 724, 819, 1145, 1447
AluI AGCT 7 cut(s) 285, 470, 614, 724, 819, 1145, 1447
Alw21I GWGCWC 1 cut(s) 1449
Alw26I GTCTC 4 cut(s) 441, 1339, 1434, 1710
AlwI GGATC 3 cut(s) 1109, 1297, 1310
AlwNI CAGNNNCTG 2 cut(s) 229, 1700
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 8 cut(s) 4, 20, 139, 173, 614, 617, 881, 1737
ApoI RAATTY 2 cut(s) 560, 806
Asp700I GAANNNNTTC 2 cut(s) 737, 1336
AspA2I CCTAGG 1 cut(s) 265
AspS9I GGNCC 2 cut(s) 596, 1022
AsuC2I CCSGG 3 cut(s) 340, 380, 1219
AsuHPI GGTGA 4 cut(s) 227, 523, 872, 1223
AvaII GGWCC 2 cut(s) 596, 1022
AvrII CCTAGG 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 211
BamHI GGATCC 1 cut(s) 1302
BanII GRGCYC 1 cut(s) 1449
BbrPI CACGTG 1 cut(s) 1073
BbsI GAAGAC 2 cut(s) 93, 666
Bbv12I GWGCWC 1 cut(s) 1449
BbvCI CCTCAGC 2 cut(s) 199, 205
BbvI GCAGC 7 cut(s) 7, 126, 160, 601, 604, 868, 1724
BccI CCATC 3 cut(s) 386, 563, 1202
BceAI ACGGC 1 cut(s) 1640
BciT130I CCWGG 3 cut(s) 641, 1373, 1701
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 3 cut(s) 340, 380, 1219
BcoDI GTCTC 4 cut(s) 441, 1339, 1434, 1710
BfaI CTAG 5 cut(s) 266, 441, 795, 1508, 1620
BfmI CTRYAG 1 cut(s) 1728
BfrI CTTAAG 1 cut(s) 161
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 9 cut(s) 5, 21, 140, 174, 615, 618, 882, 993, 1738
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 9 cut(s) 6, 22, 141, 175, 616, 619, 883, 994, 1739
Bme1390I CCNGG 6 cut(s) 340, 380, 641, 1219, 1373, 1701
Bme18I GGWCC 2 cut(s) 596, 1022
BmgT120I GGNCC 2 cut(s) 596, 1022
BmiI GGNNCC 2 cut(s) 1023, 1304
BmrFI CCNGG 6 cut(s) 340, 380, 641, 1219, 1373, 1701
BoxI GACNNNNGTC 1 cut(s) 900
BpiI GAAGAC 2 cut(s) 93, 666
BpmI CTGGAG 1 cut(s) 570
Bpu10I CCTNAGC 2 cut(s) 199, 205
BpuEI CTTGAG 3 cut(s) 1289, 1328, 1380
BpuMI CCSGG 3 cut(s) 340, 380, 1219
BsaAI YACGTR 1 cut(s) 1073
BsaI GGTCTC 1 cut(s) 441
BsaJI CCNNGG 6 cut(s) 168, 265, 306, 339, 1371, 1656
BsaXI ACNNNNNCTCC 4 cut(s) 1584, 1614, 1671, 1701
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 1208
Bse1I ACTGG 4 cut(s) 51, 147, 553, 1118
Bse21I CCTNAGG 1 cut(s) 211
Bse3DI GCAATG 2 cut(s) 927, 1501
BseBI CCWGG 3 cut(s) 641, 1373, 1701
BseDI CCNNGG 6 cut(s) 168, 265, 306, 339, 1371, 1656
BseGI GGATG 3 cut(s) 574, 706, 1227
BseLI CCNNNNNNNGG 2 cut(s) 267, 1208
BseMI GCAATG 2 cut(s) 927, 1501
BseMII CTCAG 9 cut(s) 136, 213, 219, 225, 900, 1289, 1334, 1392, 1462
BseNI ACTGG 4 cut(s) 51, 147, 553, 1118
BseRI GAGGAG 3 cut(s) 121, 1383, 1610
BseXI GCAGC 7 cut(s) 7, 126, 160, 601, 604, 868, 1724
BsgI GTGCAG 1 cut(s) 125
BshFI GGCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 1449
BsiSI CCGG 3 cut(s) 339, 380, 1219
BslFI GGGAC 1 cut(s) 831
BslI CCNNNNNNNGG 2 cut(s) 267, 1208
BsmAI GTCTC 4 cut(s) 441, 1339, 1434, 1710
BsmBI CGTCTC 1 cut(s) 1710
BsmFI GGGAC 1 cut(s) 831
BsnI GGCC 1 cut(s) 167
Bso31I GGTCTC 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 1449
Bsp143I GATC 3 cut(s) 703, 1114, 1302
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 2 cut(s) 992, 1059
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 9 cut(s) 135, 212, 218, 224, 901, 1290, 1335, 1391, 1461
BspLI GGNNCC 2 cut(s) 1023, 1304
BspPI GGATC 3 cut(s) 1109, 1297, 1310
BspQI GCTCTTC 1 cut(s) 71
BspTI CTTAAG 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 441
BsrDI GCAATG 2 cut(s) 927, 1501
BsrI ACTGG 4 cut(s) 51, 147, 553, 1118
BssECI CCNNGG 6 cut(s) 168, 265, 306, 339, 1371, 1656
BssMI GATC 3 cut(s) 703, 1114, 1302
BssT1I CCWWGG 2 cut(s) 168, 265
Bst2UI CCWGG 3 cut(s) 641, 1373, 1701
Bst4CI ACNGT 2 cut(s) 826, 1523
Bst6I CTCTTC 2 cut(s) 71, 439
BstAFI CTTAAG 1 cut(s) 161
BstAPI GCANNNNNTGC 1 cut(s) 1463
BstBAI YACGTR 1 cut(s) 1073
BstC8I GCNNGC 5 cut(s) 34, 225, 726, 1101, 1315
BstDSI CCRYGG 3 cut(s) 168, 306, 1656
BstF5I GGATG 3 cut(s) 574, 706, 1227
BstKTI GATC 3 cut(s) 706, 1117, 1305
BstMAI GTCTC 4 cut(s) 441, 1339, 1434, 1710
BstMBI GATC 3 cut(s) 703, 1114, 1302
BstNI CCWGG 3 cut(s) 641, 1373, 1701
BstNSI RCATGY 3 cut(s) 749, 1487, 1671
BstPAI GACNNNNGTC 1 cut(s) 900
BstSCI CCNGG 6 cut(s) 338, 378, 639, 1217, 1371, 1699
BstSFI CTRYAG 1 cut(s) 1728
BstV1I GCAGC 7 cut(s) 7, 126, 160, 601, 604, 868, 1724
BstV2I GAAGAC 2 cut(s) 93, 666
BstX2I RGATCY 2 cut(s) 1114, 1302
BstXI CCANNNNNNTGG 1 cut(s) 1644
BstYI RGATCY 2 cut(s) 1114, 1302
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 1 cut(s) 167
BtgI CCRYGG 3 cut(s) 168, 306, 1656
BtsCI GGATG 3 cut(s) 574, 706, 1227
BtsIMutI CAGTG 3 cut(s) 1125, 1340, 1456
Cac8I GCNNGC 5 cut(s) 34, 225, 726, 1101, 1315
CaiI CAGNNNCTG 2 cut(s) 229, 1700
Cfr13I GGNCC 2 cut(s) 596, 1022
CsiI ACCWGGT 1 cut(s) 1699
Csp6I GTAC 1 cut(s) 1505
CviQI GTAC 1 cut(s) 1505
DpnI GATC 3 cut(s) 705, 1116, 1304
DpnII GATC 3 cut(s) 703, 1114, 1302
Eam1104I CTCTTC 2 cut(s) 71, 439
EarI CTCTTC 2 cut(s) 71, 439
Ecl136II GAGCTC 1 cut(s) 1447
Eco130I CCWWGG 2 cut(s) 168, 265
Eco24I GRGCYC 1 cut(s) 1449
Eco31I GGTCTC 1 cut(s) 441
Eco32I GATATC 2 cut(s) 234, 1402
Eco47I GGWCC 2 cut(s) 596, 1022
Eco53kI GAGCTC 1 cut(s) 1447
Eco57I CTGAAG 2 cut(s) 263, 1768
Eco72I CACGTG 1 cut(s) 1073
Eco81I CCTNAGG 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 1447
EcoO109I RGGNCCY 1 cut(s) 1022
EcoRII CCWGG 3 cut(s) 639, 1371, 1699
EcoRV GATATC 2 cut(s) 234, 1402
EcoT14I CCWWGG 2 cut(s) 168, 265
EcoT38I GRGCYC 1 cut(s) 1449
ErhI CCWWGG 2 cut(s) 168, 265
Esp3I CGTCTC 1 cut(s) 1710
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 1 cut(s) 831
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 1 cut(s) 154
Fnu4HI GCNGC 9 cut(s) 5, 21, 140, 174, 615, 618, 882, 993, 1738
FokI GGATG 3 cut(s) 581, 713, 1234
FriOI GRGCYC 1 cut(s) 1449
Fsp4HI GCNGC 9 cut(s) 5, 21, 140, 174, 615, 618, 882, 993, 1738
FspBI CTAG 5 cut(s) 266, 441, 795, 1508, 1620
GluI GCNGC 9 cut(s) 5, 21, 140, 174, 615, 618, 882, 993, 1738
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 1 cut(s) 167
HapII CCGG 3 cut(s) 339, 380, 1219
HincII GTYRAC 1 cut(s) 895
HindII GTYRAC 1 cut(s) 895
HindIII AAGCTT 2 cut(s) 722, 817
HinfI GANTC 9 cut(s) 514, 573, 625, 653, 761, 901, 1294, 1376, 1552
HpaII CCGG 3 cut(s) 339, 380, 1219
HphI GGTGA 4 cut(s) 227, 523, 872, 1223
Hpy166II GTNNAC 5 cut(s) 119, 155, 694, 895, 1544
Hpy188III TCNNGA 5 cut(s) 124, 1268, 1397, 1489, 1559
Hpy8I GTNNAC 5 cut(s) 119, 155, 694, 895, 1544
Hpy99I CGWCG 1 cut(s) 1560
HpyAV CCTTC 5 cut(s) 271, 727, 860, 949, 1639
HpyCH4III ACNGT 2 cut(s) 826, 1523
HpyCH4IV ACGT 1 cut(s) 1072
HpySE526I ACGT 1 cut(s) 1072
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 3 cut(s) 703, 1114, 1302
LguI GCTCTTC 1 cut(s) 71
Lsp1109I GCAGC 7 cut(s) 7, 126, 160, 601, 604, 868, 1724
MabI ACCWGGT 1 cut(s) 1699
MaeI CTAG 5 cut(s) 266, 441, 795, 1508, 1620
MaeII ACGT 1 cut(s) 1072
MaeIII GTNAC 2 cut(s) 349, 538
MalI GATC 3 cut(s) 705, 1116, 1304
MboI GATC 3 cut(s) 703, 1114, 1302
MfeI CAATTG 1 cut(s) 1500
MflI RGATCY 2 cut(s) 1114, 1302
MhlI GDGCHC 1 cut(s) 1449
MluCI AATT 9 cut(s) 188, 297, 486, 560, 710, 806, 1000, 1406, 1500
MlyI GAGTC 3 cut(s) 910, 1288, 1370
MmeI TCCRAC 1 cut(s) 766
MroXI GAANNNNTTC 2 cut(s) 737, 1336
MseI TTAA 9 cut(s) 162, 402, 687, 821, 915, 1388, 1410, 1568, 1792
MslI CAYNNNNRTG 3 cut(s) 633, 1471, 1772
MspCI CTTAAG 1 cut(s) 161
MspI CCGG 3 cut(s) 339, 380, 1219
MspR9I CCNGG 6 cut(s) 340, 380, 641, 1219, 1373, 1701
MunI CAATTG 1 cut(s) 1500
MvaI CCWGG 3 cut(s) 641, 1373, 1701
NciI CCSGG 3 cut(s) 340, 380, 1219
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 703, 1114, 1302
NlaIV GGNNCC 2 cut(s) 1023, 1304
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 3 cut(s) 749, 1487, 1671
PciI ACATGT 2 cut(s) 745, 1483
PciSI GCTCTTC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 737, 1336
PfeI GAWTC 6 cut(s) 514, 573, 625, 653, 761, 1552
PflMI CCANNNNNTGG 1 cut(s) 1208
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 9 cut(s) 6, 22, 141, 175, 616, 619, 883, 994, 1739
PleI GAGTC 3 cut(s) 909, 1288, 1370
PmaCI CACGTG 1 cut(s) 1073
PmlI CACGTG 1 cut(s) 1073
PpsI GAGTC 3 cut(s) 909, 1288, 1370
Ppu21I YACGTR 1 cut(s) 1073
PpuMI RGGWCCY 1 cut(s) 1022
PscI ACATGT 2 cut(s) 745, 1483
PshAI GACNNNNGTC 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 1449
Psp5II RGGWCCY 1 cut(s) 1022
Psp6I CCWGG 3 cut(s) 639, 1371, 1699
PspCI CACGTG 1 cut(s) 1073
PspGI CCWGG 3 cut(s) 639, 1371, 1699
PspN4I GGNNCC 2 cut(s) 1023, 1304
PspPI GGNCC 2 cut(s) 596, 1022
PspPPI RGGWCCY 1 cut(s) 1022
PstNI CAGNNNCTG 2 cut(s) 229, 1700
PsuI RGATCY 2 cut(s) 1114, 1302
RsaI GTAC 1 cut(s) 1506
RsaNI GTAC 1 cut(s) 1505
RseI CAYNNNNRTG 3 cut(s) 633, 1471, 1772
SacI GAGCTC 1 cut(s) 1449
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 9 cut(s) 162, 402, 687, 821, 915, 1388, 1410, 1568, 1792
SatI GCNGC 9 cut(s) 5, 21, 140, 174, 615, 618, 882, 993, 1738
Sau3AI GATC 3 cut(s) 703, 1114, 1302
Sau96I GGNCC 2 cut(s) 596, 1022
SchI GAGTC 3 cut(s) 910, 1288, 1370
ScrFI CCNGG 6 cut(s) 340, 380, 641, 1219, 1373, 1701
SduI GDGCHC 1 cut(s) 1449
SexAI ACCWGGT 1 cut(s) 1699
SfcI CTRYAG 1 cut(s) 1728
SinI GGWCC 2 cut(s) 596, 1022
SmiMI CAYNNNNRTG 3 cut(s) 633, 1471, 1772
SmlI CTYRAG 4 cut(s) 161, 1268, 1307, 1395
SmoI CTYRAG 4 cut(s) 161, 1268, 1307, 1395
Sse9I AATT 9 cut(s) 188, 297, 486, 560, 710, 806, 1000, 1406, 1500
SsiI CCGC 2 cut(s) 992, 1059
SspMI CTAG 5 cut(s) 266, 441, 795, 1508, 1620
SstI GAGCTC 1 cut(s) 1449
StyD4I CCNGG 6 cut(s) 338, 378, 639, 1217, 1371, 1699
StyI CCWWGG 2 cut(s) 168, 265
TaaI ACNGT 2 cut(s) 826, 1523
TaiI ACGT 1 cut(s) 1075
TaqI TCGA 2 cut(s) 390, 576
TasI AATT 9 cut(s) 188, 297, 486, 560, 710, 806, 1000, 1406, 1500
TatI WGTACW 1 cut(s) 1504
TauI GCSGC 1 cut(s) 995
TfiI GAWTC 6 cut(s) 514, 573, 625, 653, 761, 1552
Tru1I TTAA 9 cut(s) 162, 402, 687, 821, 915, 1388, 1410, 1568, 1792
Tru9I TTAA 9 cut(s) 162, 402, 687, 821, 915, 1388, 1410, 1568, 1792
TscAI CASTG 3 cut(s) 1125, 1347, 1456
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 8 cut(s) 4, 20, 139, 173, 614, 617, 881, 1737
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 2 cut(s) 498, 1734
TspRI CASTG 3 cut(s) 1125, 1347, 1456
Van91I CCANNNNNTGG 1 cut(s) 1208
Vha464I CTTAAG 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 596, 1022
XapI RAATTY 2 cut(s) 560, 806
XceI RCATGY 3 cut(s) 749, 1487, 1671
XmaJI CCTAGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 154
XmnI GAANNNNTTC 2 cut(s) 737, 1336
XspI CTAG 5 cut(s) 266, 441, 795, 1508, 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.