MD12G1088900.v1.1

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002841
Physical Location & Seq
Reverse (-)
10996096 .. 10998603
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1088900.v1.1.491

Sequence Viewer

Length: 423 bp
ATGTTGCTCAAAAGTCCCCTTCGTTATGGTGAAAATCCTCATCAGAAGGCTGCATTCTACGTTGACAAGAGTCTTTCTGAGGTGAATGCTGGTGGTATTGCAACTGCTATCCGACACCATGGGAAGGAAATGTCATATAATAACTATTTAGATGCCGATGCGGCTTGGAATTGTGTGTCAGAGTTTCAGAGTCCTACCTGTGTAGTTGTAAAGCATACAAATCCTTGTGGTGTAGCTTCACGTGATGATATTCTTGAAGCATACAGGAGATCCCGTGAGTGCTTTTGGTGGCATTGTAGCGCTCTTGCTAAGGAAATCCGAGAGTTTAGAAGCCTGACAGATGGTGAAACTCGGATGTTTTACGAGATTGTGGTTGCACCCAAGTACACAAAGAAGGGGCCTGAAATCCTCCGCGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

141

Amino Acids

15.98

Weight (kDa)

8.59

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AICARFT_IMPCHas PF01808 5 - 140 7.2e-39 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 414
AciI CCGC 3 cut(s) 161, 412, 414
AclWI GGATC 1 cut(s) 264
AcvI CACGTG 1 cut(s) 242
AfaI GTAC 1 cut(s) 386
AfeI AGCGCT 1 cut(s) 301
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 1 cut(s) 257
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
AlwI GGATC 1 cut(s) 264
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 1 cut(s) 398
ApeKI GCWGC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 3 cut(s) 41, 94, 356
BbrPI CACGTG 1 cut(s) 242
BbvI GCAGC 1 cut(s) 37
BccI CCATC 1 cut(s) 335
BfoI RGCGCY 1 cut(s) 303
BglI GCCNNNNNGGC 1 cut(s) 161
BisI GCNGC 2 cut(s) 51, 162
BlsI GCNGC 2 cut(s) 52, 163
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 399
BmsI GCATC 2 cut(s) 142, 148
BoxI GACNNNNGTC 1 cut(s) 69
Bpu10I CCTNAGC 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 242
BsaJI CCNNGG 2 cut(s) 118, 412
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 118, 412
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 124
BsmI GAATGC 2 cut(s) 53, 91
BsnI GGCC 1 cut(s) 400
Bsp143I GATC 1 cut(s) 269
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 3 cut(s) 161, 412, 414
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 1 cut(s) 264
BssECI CCNNGG 2 cut(s) 118, 412
BssMI GATC 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 78, 309
BstDSI CCRYGG 2 cut(s) 118, 412
BstF5I GGATG 1 cut(s) 360
BstFNI CGCG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 1 cut(s) 272
BstMBI GATC 1 cut(s) 269
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstPAI GACNNNNGTC 1 cut(s) 69
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuRI GGCC 1 cut(s) 400
BtgI CCRYGG 2 cut(s) 118, 412
BtsCI GGATG 1 cut(s) 360
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 398
Cfr42I CCGCGG 1 cut(s) 415
Csp6I GTAC 1 cut(s) 385
CviAII CATG 1 cut(s) 119
CviJI RGCY 5 cut(s) 50, 164, 236, 333, 400
CviKI_1 RGCY 5 cut(s) 50, 164, 236, 333, 400
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 78, 309
DpnI GATC 1 cut(s) 271
DpnII GATC 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 118
Eco47III AGCGCT 1 cut(s) 301
Eco72I CACGTG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 122
FaiI YATR 6 cut(s) 27, 120, 136, 138, 216, 262
FatI CATG 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 51, 162
FokI GGATG 1 cut(s) 367
Fsp4HI GCNGC 2 cut(s) 51, 162
GlaI GCGC 1 cut(s) 301
GluI GCNGC 2 cut(s) 51, 162
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 1 cut(s) 400
HhaI GCGC 1 cut(s) 302
Hin1II CATG 1 cut(s) 122
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 2 cut(s) 70, 190
HphI GGTGA 3 cut(s) 41, 94, 356
Hpy166II GTNNAC 2 cut(s) 64, 387
Hpy188I TCNGA 7 cut(s) 45, 79, 113, 181, 189, 320, 354
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 2 cut(s) 64, 387
HpyAV CCTTC 4 cut(s) 29, 40, 118, 388
HpyCH4IV ACGT 2 cut(s) 60, 241
HpyCH4V TGCA 3 cut(s) 53, 101, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 2 cut(s) 78, 309
HpySE526I ACGT 2 cut(s) 60, 241
Hsp92II CATG 1 cut(s) 122
HspAI GCGC 1 cut(s) 300
KspI CCGCGG 1 cut(s) 415
Kzo9I GATC 1 cut(s) 269
LpnPI CCDG 5 cut(s) 75, 211, 250, 347, 414
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 2 cut(s) 142, 148
MaeII ACGT 2 cut(s) 60, 241
MalI GATC 1 cut(s) 271
MboI GATC 1 cut(s) 269
MflI RGATCY 1 cut(s) 269
MluCI AATT 1 cut(s) 169
MlyI GAGTC 2 cut(s) 79, 199
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 3 cut(s) 48, 73, 419
MspA1I CMGCKG 1 cut(s) 414
Mva1269I GAATGC 2 cut(s) 53, 91
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 161
NcoI CCATGG 1 cut(s) 118
NdeII GATC 1 cut(s) 269
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 1 cut(s) 399
PctI GAATGC 2 cut(s) 53, 91
PkrI GCNGC 2 cut(s) 52, 163
PleI GAGTC 2 cut(s) 78, 198
PmaCI CACGTG 1 cut(s) 242
PmlI CACGTG 1 cut(s) 242
PpsI GAGTC 2 cut(s) 78, 198
Ppu21I YACGTR 1 cut(s) 242
PshAI GACNNNNGTC 1 cut(s) 69
PspCI CACGTG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 1 cut(s) 398
PsuI RGATCY 1 cut(s) 269
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SacII CCGCGG 1 cut(s) 415
SatI GCNGC 2 cut(s) 51, 162
Sau3AI GATC 1 cut(s) 269
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 2 cut(s) 79, 199
SetI ASST 5 cut(s) 63, 84, 200, 238, 244
SfaNI GCATC 2 cut(s) 142, 148
Sfr303I CCGCGG 1 cut(s) 415
SgrBI CCGCGG 1 cut(s) 415
Sse9I AATT 1 cut(s) 169
SsiI CCGC 3 cut(s) 161, 412, 414
StyI CCWWGG 1 cut(s) 118
TaiI ACGT 2 cut(s) 63, 244
TasI AATT 1 cut(s) 169
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 164
TseI GCWGC 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.