pycom12g03130

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002841
Physical Location & Seq
Reverse (-)
2826418 .. 2830546
4129 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03130.11

Sequence Viewer

Length: 1839 bp
ATGCTCGGTCTGACTGCCTCTCCGGCCGCCGCAACGCCACCTAACTCCAACACCACAAATTCTTGTCTTATTCCAAACACCACCAGTCTTCTCCTGCTATCCTCCAACGCCCAAATAAGAACCAATTCCTCCCCTTCTTTCACGTGTTGCTCAGCGAGGAACTTGCACTCAAGTTGCAGAACAATTAAAGCCATGGCGGTGACTGAGGAGACCCTTTCAGTCAGTAACCCTCCCTCAACCCTTTCTGGTAATAGGCAGGCTCTGATATCATTGTCAGACAAGAAGGATCTTGCTATCTTAGGGAATGGCCTTCAGAACTTGGGATATAAAATTGTTTCGACTGGAGGAACGGCATCTGCTTTAGAAAGTGCTGGCCTGCCCGTCACTAAGGTGGAACAACTTACTTGTTTCCCGGAGATGCTTGATGGTCGTGTAAAAACTTTACACCCCAATGTGCATGGGGGTATCCTTGCTAGAAGAGACCAAAAGCATCACATGGAAGCTCTTAGTATGCACGGAATTGGTACATTTGATGTAGTTGTGGTGAATTTGTACCCCTTTTACGAGAAAGTTACTTCAACAAGAGGAATAGAGTTTGAAGATGGAATTGAGAATATAGATATTGGTGGTCCTGCCATGATAAGAGCGGCTGCAAAGAATCACAAAGATGTCTTGGTGGTGGTTGATTCAGAAGACTATCCCGCACTTATAGAGTTTCTTAAGGGAGACAAGGATGATCAACAATTCCGAAGGAAGCTTGCATGGAAGGCTTTCCAACATGTTGCTTCTTATGATTCTGCAGTTTCTGAATGGCTTTGGAAGCAGACTTCTGAAGATAAATTCCCTCAGAGCTTAACGGTGCCTATGTTGCTCAAAAGTCCCCTTCGTTATGGTGAAAATCCTCATCAGAAGGCTGCATTCTATGTTGACAAGAGTCTTTCTGAGGTGAATGCTGGTGGTATTGCAACTGCTATCCAACACCATGGGAAGGAAATGTCATATAATAACTATCTAGATGCAGATGCGGCTTGGAATTGTGTGTCAGAGTTTCAAAGTCCTACCTGTGTAGTCGTAAAGCATACAAATCCCTGTGGTGTAGCTTCACGTGATGATATTCTTGAAGCATACAGGCTTGCTGTCAAAGCAGATCCCGTGAGTGCATTTGGTGGCATTGTAGCGTTCAACATAGAGGTTGATGAGGCTCTTGCTAAGGAAATTCGAGAGTTTAGAAGCCCGCCAGATGGCGAAACCCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTACACAAAGAAAGGGCTTGAAATCCTTCGCGGAAAATCAAAGACTCTAAGGATCCTTGAGGCAAGAAAAAATGAGAAGGGAAAGCTTTCACTTAGACAGGTTGGTGGTGGGTGGTTAGCCCAGGACTCGGATGATTTGACCCCCCAGGATATACAGTTTAAAGCTGTTTCCGAGAAGGCTCCACAAAAAAGTGAGCTTGCTGATGCAGAGTTCGCATGGTTGTGCGTCAAGCATGTTAAAAGCAATGCAATTGTAATAGCAAAGGTCGTCTTTCTCTGCTTCACCTTTCAAAATCTCGCCTGTGGGCAGCCTAACCGCCTGGAGAGTTTACGAATAGCCTTGAAGAAAGCTGGAGAAGAGGTCAAGGGTGCAGCTTTGGCCAGCGATGCCTTCTTCCCATTCGCATGGAAAGATGCAGTGGAAGAAGCCTGCGAAAGCGGAGTTAGTGTAATTGCTGAACCTGGTGGGAGCATTAGAGATGGGGATGCCATAGACTGCTGTAACAAGTATGGAGTTTCACTTCTTTTCACAAACGTTAGGCACTTCAGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

613

Amino Acids

66.8

Weight (kDa)

6.59

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 859
AccBSI CCGCTC 1 cut(s) 647
AccII CGCG 1 cut(s) 1314
AclI AACGTT 1 cut(s) 1818
AclWI GGATC 4 cut(s) 294, 1142, 1330, 1343
AcoI YGGCCR 2 cut(s) 24, 1662
AcsI RAATTY 4 cut(s) 58, 547, 839, 1215
AcuI CTGAAG 3 cut(s) 296, 852, 1813
AcvI CACGTG 2 cut(s) 144, 1106
AfaI GTAC 3 cut(s) 526, 554, 1286
AfiI CCNNNNNNNGG 3 cut(s) 988, 1241, 1411
AflII CTTAAG 1 cut(s) 719
AflIII ACRYGT 2 cut(s) 143, 778
AgsI TTSAA 8 cut(s) 579, 599, 1052, 1121, 1183, 1304, 1574, 1627
AjnI CCWGG 4 cut(s) 1404, 1428, 1602, 1744
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 9 cut(s) 503, 757, 852, 1100, 1369, 1448, 1480, 1634, 1658
AluI AGCT 9 cut(s) 503, 757, 852, 1100, 1369, 1448, 1480, 1634, 1658
Alw26I GTCTC 3 cut(s) 203, 474, 720
AlwI GGATC 4 cut(s) 294, 1142, 1330, 1343
AlwNI CAGNNNCTG 2 cut(s) 262, 806
AoxI GGCC 4 cut(s) 24, 307, 373, 1662
ApeKI GCWGC 4 cut(s) 650, 914, 1591, 1655
ApoI RAATTY 4 cut(s) 58, 547, 839, 1215
Asp700I GAANNNNTTC 4 cut(s) 124, 770, 1308, 1369
AspS9I GGNCC 1 cut(s) 629
AsuC2I CCSGG 2 cut(s) 413, 1252
AsuHPI GGTGA 5 cut(s) 211, 556, 905, 958, 1558
AvaII GGWCC 1 cut(s) 629
BalI TGGCCA 1 cut(s) 1664
BamHI GGATCC 1 cut(s) 1335
BanI GGYRCC 1 cut(s) 859
BbrPI CACGTG 2 cut(s) 144, 1106
BbsI GAAGAC 2 cut(s) 80, 699
BbvI GCAGC 4 cut(s) 637, 901, 1603, 1667
BccI CCATC 4 cut(s) 419, 596, 1235, 1757
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 4 cut(s) 1406, 1430, 1604, 1746
BciVI GTATCC 1 cut(s) 476
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 2 cut(s) 413, 1252
BcoDI GTCTC 3 cut(s) 203, 474, 720
BfaI CTAG 2 cut(s) 474, 1013
BfmI CTRYAG 1 cut(s) 798
BfrI CTTAAG 1 cut(s) 719
BfuI GTATCC 1 cut(s) 476
BglI GCCNNNNNGGC 1 cut(s) 23
BisI GCNGC 8 cut(s) 27, 30, 648, 651, 915, 1026, 1592, 1656
BlpI GCTNAGC 1 cut(s) 151
BlsI GCNGC 8 cut(s) 28, 31, 649, 652, 916, 1027, 1593, 1657
Bme1390I CCNGG 6 cut(s) 413, 1252, 1406, 1430, 1604, 1746
Bme18I GGWCC 1 cut(s) 629
BmgT120I GGNCC 1 cut(s) 629
BmiI GGNNCC 3 cut(s) 861, 1337, 1464
BmrFI CCNGG 6 cut(s) 413, 1252, 1406, 1430, 1604, 1746
BmsI GCATC 9 cut(s) 362, 408, 499, 1006, 1012, 1477, 1660, 1687, 1759
BoxI GACNNNNGTC 1 cut(s) 933
BpiI GAAGAC 2 cut(s) 80, 699
BpmI CTGGAG 3 cut(s) 363, 1625, 1656
Bpu10I CCTNAGC 1 cut(s) 1209
Bpu1102I GCTNAGC 1 cut(s) 151
BpuEI CTTGAG 2 cut(s) 154, 1361
BpuMI CCSGG 2 cut(s) 413, 1252
BsaAI YACGTR 2 cut(s) 144, 1106
BsaI GGTCTC 2 cut(s) 203, 474
BsaJI CCNNGG 4 cut(s) 192, 982, 1404, 1428
BsaXI ACNNNNNCTCC 5 cut(s) 34, 1629, 1659, 1716, 1746
Bsc4I CCNNNNNNNGG 3 cut(s) 988, 1241, 1411
Bse1I ACTGG 2 cut(s) 84, 346
Bse3DI GCAATG 1 cut(s) 1534
BseBI CCWGG 4 cut(s) 1406, 1430, 1604, 1746
BseDI CCNNGG 4 cut(s) 192, 982, 1404, 1428
BseGI GGATG 4 cut(s) 739, 1260, 1420, 1774
BseLI CCNNNNNNNGG 3 cut(s) 988, 1241, 1411
BseMI GCAATG 1 cut(s) 1534
BseMII CTCAG 4 cut(s) 165, 195, 860, 933
BseNI ACTGG 2 cut(s) 84, 346
BseRI GAGGAG 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 4 cut(s) 637, 901, 1603, 1667
BsgI GTGCAG 1 cut(s) 1674
Bsh1236I CGCG 1 cut(s) 1314
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 4 cut(s) 26, 309, 375, 1664
BshNI GGYRCC 1 cut(s) 859
BsiEI CGRYCG 1 cut(s) 27
BsiSI CCGG 3 cut(s) 23, 413, 1252
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 3 cut(s) 988, 1241, 1411
BsmAI GTCTC 3 cut(s) 203, 474, 720
BsmFI GGGAC 1 cut(s) 864
BsmI GAATGC 2 cut(s) 917, 955
BsnI GGCC 4 cut(s) 26, 309, 375, 1664
Bso31I GGTCTC 2 cut(s) 203, 474
Bsp143I GATC 4 cut(s) 286, 736, 1147, 1335
Bsp1720I GCTNAGC 1 cut(s) 151
Bsp19I CCATGG 2 cut(s) 192, 982
BspANI GGCC 4 cut(s) 26, 309, 375, 1664
BspCNI CTCAG 4 cut(s) 164, 196, 859, 934
BspFNI CGCG 1 cut(s) 1314
BspLI GGNNCC 3 cut(s) 861, 1337, 1464
BspMAI CTGCAG 1 cut(s) 802
BspPI GGATC 4 cut(s) 294, 1142, 1330, 1343
BspT107I GGYRCC 1 cut(s) 859
BspTI CTTAAG 1 cut(s) 719
BspTNI GGTCTC 2 cut(s) 203, 474
BsrBI CCGCTC 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 1534
BsrI ACTGG 2 cut(s) 84, 346
BssECI CCNNGG 4 cut(s) 192, 982, 1404, 1428
BssMI GATC 4 cut(s) 286, 736, 1147, 1335
BssT1I CCWWGG 2 cut(s) 192, 982
Bst2UI CCWGG 4 cut(s) 1406, 1430, 1604, 1746
Bst4CI ACNGT 2 cut(s) 859, 1440
Bst6I CTCTTC 2 cut(s) 472, 1635
BstAFI CTTAAG 1 cut(s) 719
BstBAI YACGTR 2 cut(s) 144, 1106
BstC8I GCNNGC 9 cut(s) 258, 373, 377, 759, 1134, 1235, 1482, 1666, 1714
BstDSI CCRYGG 2 cut(s) 192, 982
BstF5I GGATG 4 cut(s) 739, 1260, 1420, 1774
BstFNI CGCG 1 cut(s) 1314
BstKTI GATC 4 cut(s) 289, 739, 1150, 1338
BstMAI GTCTC 3 cut(s) 203, 474, 720
BstMBI GATC 4 cut(s) 286, 736, 1147, 1335
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 4 cut(s) 1406, 1430, 1604, 1746
BstNSI RCATGY 2 cut(s) 782, 1520
BstPAI GACNNNNGTC 1 cut(s) 933
BstSCI CCNGG 6 cut(s) 411, 1250, 1404, 1428, 1602, 1744
BstSFI CTRYAG 1 cut(s) 798
BstUI CGCG 1 cut(s) 1314
BstV1I GCAGC 4 cut(s) 637, 901, 1603, 1667
BstV2I GAAGAC 2 cut(s) 80, 699
BstX2I RGATCY 3 cut(s) 286, 1147, 1335
BstXI CCANNNNNNTGG 2 cut(s) 983, 1689
BstYI RGATCY 3 cut(s) 286, 1147, 1335
BstZI CGGCCG 1 cut(s) 24
BsuI GTATCC 1 cut(s) 476
BsuRI GGCC 4 cut(s) 26, 309, 375, 1664
BtgI CCRYGG 2 cut(s) 192, 982
BtgZI GCGATG 1 cut(s) 1683
BtsCI GGATG 4 cut(s) 739, 1260, 1420, 1774
BtsI GCAGTG 1 cut(s) 1707
BtsIMutI CAGTG 1 cut(s) 1707
Cac8I GCNNGC 9 cut(s) 258, 373, 377, 759, 1134, 1235, 1482, 1666, 1714
CaiI CAGNNNCTG 2 cut(s) 262, 806
Cfr13I GGNCC 1 cut(s) 629
CseI GACGC 1 cut(s) 1498
CsiI ACCWGGT 1 cut(s) 1744
Csp6I GTAC 3 cut(s) 525, 553, 1285
CviQI GTAC 3 cut(s) 525, 553, 1285
DpnI GATC 4 cut(s) 288, 738, 1149, 1337
DpnII GATC 4 cut(s) 286, 736, 1147, 1335
DraI TTTAAA 1 cut(s) 1444
EaeI YGGCCR 2 cut(s) 24, 1662
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 472, 1635
EarI CTCTTC 2 cut(s) 472, 1635
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 192, 982
Eco31I GGTCTC 2 cut(s) 203, 474
Eco32I GATATC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 629
Eco52I CGGCCG 1 cut(s) 24
Eco57I CTGAAG 3 cut(s) 296, 852, 1813
Eco72I CACGTG 2 cut(s) 144, 1106
EcoRII CCWGG 4 cut(s) 1404, 1428, 1602, 1744
EcoRV GATATC 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 192, 982
ErhI CCWWGG 2 cut(s) 192, 982
FalI AAGNNNNNCTT 2 cut(s) 1538, 1570
FaqI GGGAC 1 cut(s) 864
FauI CCCGC 2 cut(s) 709, 1242
FbaI TGATCA 1 cut(s) 736
Fnu4HI GCNGC 8 cut(s) 27, 30, 648, 651, 915, 1026, 1592, 1656
FokI GGATG 4 cut(s) 746, 1267, 1427, 1781
Fsp4HI GCNGC 8 cut(s) 27, 30, 648, 651, 915, 1026, 1592, 1656
FspBI CTAG 2 cut(s) 474, 1013
GluI GCNGC 8 cut(s) 27, 30, 648, 651, 915, 1026, 1592, 1656
GsuI CTGGAG 3 cut(s) 363, 1625, 1656
HaeIII GGCC 4 cut(s) 26, 309, 375, 1664
HapII CCGG 3 cut(s) 23, 413, 1252
HgaI GACGC 1 cut(s) 1498
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 2 cut(s) 755, 1367
HinfI GANTC 6 cut(s) 658, 686, 794, 934, 1327, 1409
HpaII CCGG 3 cut(s) 23, 413, 1252
HphI GGTGA 5 cut(s) 211, 556, 905, 958, 1558
Hpy166II GTNNAC 3 cut(s) 928, 1287, 1613
Hpy188III TCNNGA 3 cut(s) 1013, 1118, 1220
Hpy8I GTNNAC 3 cut(s) 928, 1287, 1613
HpyCH4III ACNGT 2 cut(s) 859, 1440
HpyCH4IV ACGT 3 cut(s) 143, 1105, 1818
HpySE526I ACGT 3 cut(s) 143, 1105, 1818
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 4 cut(s) 286, 736, 1147, 1335
LmnI GCTCC 2 cut(s) 1468, 1752
Lsp1109I GCAGC 4 cut(s) 637, 901, 1603, 1667
LweI GCATC 9 cut(s) 362, 408, 499, 1006, 1012, 1477, 1660, 1687, 1759
MabI ACCWGGT 1 cut(s) 1744
MaeI CTAG 2 cut(s) 474, 1013
MaeII ACGT 3 cut(s) 143, 1105, 1818
MaeIII GTNAC 5 cut(s) 199, 224, 382, 571, 1784
MalI GATC 4 cut(s) 288, 738, 1149, 1337
MbiI CCGCTC 1 cut(s) 647
MboI GATC 4 cut(s) 286, 736, 1147, 1335
MboII GAAGA 9 cut(s) 80, 489, 611, 704, 845, 1639, 1652, 1669, 1718
MfeI CAATTG 1 cut(s) 1533
MflI RGATCY 3 cut(s) 286, 1147, 1335
MlsI TGGCCA 1 cut(s) 1664
MluNI TGGCCA 1 cut(s) 1664
MlyI GAGTC 3 cut(s) 943, 1321, 1403
MmeI TCCRAC 4 cut(s) 72, 129, 799, 1000
Mox20I TGGCCA 1 cut(s) 1664
MroXI GAANNNNTTC 4 cut(s) 124, 770, 1308, 1369
MscI TGGCCA 1 cut(s) 1664
MseI TTAA 5 cut(s) 186, 720, 854, 1443, 1521
MslI CAYNNNNRTG 6 cut(s) 197, 389, 450, 666, 1504, 1687
Msp20I TGGCCA 1 cut(s) 1664
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 3 cut(s) 23, 413, 1252
MspR9I CCNGG 6 cut(s) 413, 1252, 1406, 1430, 1604, 1746
MunI CAATTG 1 cut(s) 1533
Mva1269I GAATGC 2 cut(s) 917, 955
MvaI CCWGG 4 cut(s) 1406, 1430, 1604, 1746
MvnI CGCG 1 cut(s) 1314
NciI CCSGG 2 cut(s) 413, 1252
NcoI CCATGG 2 cut(s) 192, 982
NdeII GATC 4 cut(s) 286, 736, 1147, 1335
NlaIV GGNNCC 3 cut(s) 861, 1337, 1464
NmuCI GTSAC 2 cut(s) 199, 382
NspI RCATGY 2 cut(s) 782, 1520
OliI CACNNNNGTG 1 cut(s) 389
PciI ACATGT 1 cut(s) 778
PctI GAATGC 2 cut(s) 917, 955
PdmI GAANNNNTTC 4 cut(s) 124, 770, 1308, 1369
PfeI GAWTC 3 cut(s) 658, 686, 794
PfoI TCCNGGA 1 cut(s) 411
PkrI GCNGC 8 cut(s) 28, 31, 649, 652, 916, 1027, 1593, 1657
PleI GAGTC 3 cut(s) 942, 1321, 1403
PmaCI CACGTG 2 cut(s) 144, 1106
PmlI CACGTG 2 cut(s) 144, 1106
PpsI GAGTC 3 cut(s) 942, 1321, 1403
Ppu21I YACGTR 2 cut(s) 144, 1106
PscI ACATGT 1 cut(s) 778
PshAI GACNNNNGTC 1 cut(s) 933
Psp1406I AACGTT 1 cut(s) 1818
Psp6I CCWGG 4 cut(s) 1404, 1428, 1602, 1744
PspCI CACGTG 2 cut(s) 144, 1106
PspGI CCWGG 4 cut(s) 1404, 1428, 1602, 1744
PspN4I GGNNCC 3 cut(s) 861, 1337, 1464
PspPI GGNCC 1 cut(s) 629
PstI CTGCAG 1 cut(s) 802
PstNI CAGNNNCTG 2 cut(s) 262, 806
PsuI RGATCY 3 cut(s) 286, 1147, 1335
RsaI GTAC 3 cut(s) 526, 554, 1286
RsaNI GTAC 3 cut(s) 525, 553, 1285
RseI CAYNNNNRTG 6 cut(s) 197, 389, 450, 666, 1504, 1687
SaqAI TTAA 5 cut(s) 186, 720, 854, 1443, 1521
SatI GCNGC 8 cut(s) 27, 30, 648, 651, 915, 1026, 1592, 1656
Sau3AI GATC 4 cut(s) 286, 736, 1147, 1335
Sau96I GGNCC 1 cut(s) 629
SchI GAGTC 3 cut(s) 943, 1321, 1403
ScrFI CCNGG 6 cut(s) 413, 1252, 1406, 1430, 1604, 1746
SexAI ACCWGGT 1 cut(s) 1744
SfaNI GCATC 9 cut(s) 362, 408, 499, 1006, 1012, 1477, 1660, 1687, 1759
SfcI CTRYAG 1 cut(s) 798
SinI GGWCC 1 cut(s) 629
SmiMI CAYNNNNRTG 6 cut(s) 197, 389, 450, 666, 1504, 1687
SmlI CTYRAG 3 cut(s) 169, 719, 1340
SmoI CTYRAG 3 cut(s) 169, 719, 1340
SspMI CTAG 2 cut(s) 474, 1013
StyD4I CCNGG 6 cut(s) 411, 1250, 1404, 1428, 1602, 1744
StyI CCWWGG 2 cut(s) 192, 982
TaaI ACNGT 2 cut(s) 859, 1440
TaiI ACGT 3 cut(s) 146, 1108, 1821
TaqI TCGA 2 cut(s) 338, 1219
TatI WGTACW 1 cut(s) 1284
TauI GCSGC 4 cut(s) 29, 32, 650, 1028
TfiI GAWTC 3 cut(s) 658, 686, 794
Tru1I TTAA 5 cut(s) 186, 720, 854, 1443, 1521
Tru9I TTAA 5 cut(s) 186, 720, 854, 1443, 1521
TscAI CASTG 1 cut(s) 1707
TseFI GTSAC 2 cut(s) 199, 382
TseI GCWGC 4 cut(s) 650, 914, 1591, 1655
Tsp45I GTSAC 2 cut(s) 199, 382
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 1 cut(s) 1707
Vha464I CTTAAG 1 cut(s) 719
VpaK11BI GGWCC 1 cut(s) 629
XapI RAATTY 4 cut(s) 58, 547, 839, 1215
XbaI TCTAGA 1 cut(s) 1012
XceI RCATGY 2 cut(s) 782, 1520
XmnI GAANNNNTTC 4 cut(s) 124, 770, 1308, 1369
XspI CTAG 2 cut(s) 474, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.