RLG00000018812

Bifunctional purine biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002841
Physical Location & Seq
Forward (+)
36726863 .. 36732030
5168 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018812

Sequence Viewer

Length: 1791 bp
ATGCTTGGTTTGTCCGCTGCTGCTCCTGCGGCCATAACACCCAACACCAATCTTGTCCTGCTTTTCTCCACCGAACTCCCAATTACTCATTCGTCTTCTTTATCTTCTTTCACCGCGTCTTGCTCACTCAGGAACTCATCTCGCAGTTGTACTCCAATCAAAGCCATGGCTGCCCAAGAGCCTCAAGCCCCTGCCTCAGCCTCGGCCTCAGGTGATAGGCAGGCTCTGATATCTTTGTCAGACAAAAACAATCTTGATTTCTTAGGGAACGGGCTTCAGAACTTGGGATATAAAATTGTTTCCACCGGAGGAACTGCATCTGCTTTAGAAAGTGCGGGGGTGTCTGTTACAAAAGTTGAAGAAATTACTTGCTTCCCGGAGATGCTCGATGGTCGTGTCAAGACTTTGCACCCCAACATTCATGGGGGTATCCTTGCTAGAAGAGACCAAAAACATCATGTGGAAGCTCTTAATAATCATGGAATTGGTACATTCGATGTGGTTGTGGTGAATTTATATCCCTTTTATGATAAAGTTAGTTCAAAGGGAGGAATAGAATTTGAGGATGGAATCGAGAATATAGATATTGGTGGTCCTGCCATGATTAGAGCTGCTGCAAAGAATCACAAAGATGTCCTGGTGGTTGTTGATTCAGAAGACTATCCTGCACTGCTAGAATATCTTAAAGCAGACAAGGATGATCAACAATTCCGAAGAAACCTTGCGTGGAAGGCTTTTCAACATGTTGCTTCTTATGATTCTGCTGTTTCAGAGTGGCTGTGGAGGCAGACTACCGAAGATAAATTCCCGCCTAGCTTAACAGTTCCTCTATTGCTCAAAAGTCCCCTTCGGTATGGTGAAAATCCTCATCAAAAGGCTGCATTTTATGTTGATAAGAGTCTTTCTGAGGTTAATGCTGGTGGCATTGCAACTGCTATCCAACATCATGGGAAGGAGATGTCATATAATAACTATTTAGATGCTGATGCGGCTTGGAATTGTGTGTCAGAGTTCAGAAGTCCTACCTGTGTTGTTGTGAAGCATACAAATCCTTGTGGTGTAGCATCACGTGATGATATACTTGAAGCATACAGGCTTGCTGTCAAAGCAGATCCAGTGAGTGCATTTGGTGGCATTGTAGCTTTCAACATAGAGGTTGATGAGGCTCTTGCTAAAGAAATCCGAGAGTTTAGAAGCCCAACAGATGGTGAAACTCGGATGTTTTATGAGATTGTGGTTGCACCCAAGTATTCAAAGAAAGGTCTTGAAATTCTTCGTGGAAAATCAAAGACTCTGAGGATCCTCGAGGCAAGTAAAAATGAGAAAGGAAAACTTTCGCTTAGACAGGTTGGTGGTGGGTGGTTAGCCCAGGACTCGGATGATTTAAGTCCCCAAGATATCCAGTTTAATGTTGTGTCTAAGAAGTCTCCACAAGAAAGTGAGCTCAGTGATGCAGAGTTTGCATGGTTGTGCGTCAAACATGTCAAAAGCAATGCAATTGTAATAGCAAAGAATAACTGTATGTTGGGTATGGGAAGTGGACAGCCGAACCGTCGGGAGAGTTTAAGAATAGCACTGAGGAAAGCAGGAGAGGACGTCAAGGGTGCAGCTTTGGCTAGCGATGCCTTCTTCCCATTCGCTTGGAAAGATGCCGTGGAAGAAGCATGTGAAAGTGGAGTAGGTGTTATTGCAGAACCTGGTGGCAGTATAAGAGATGGAGATGCTATAGATTGCTGCAACAAGTATGGTGTTTCTCTTGTTTTCACTAATGTGAGGCACTTCAGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
AICARFT_IMPCHas MGS

Protein Analysis

597

Amino Acids

64.74

Weight (kDa)

6.05

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGS PF02142 90 - 199 1.9e-20 MGS-like domain
AICARFT_IMPCHas PF01808 205 - 528 4.3e-114 AICARFT/IMPCHase bienzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1599
AbsI CCTCGAGG 1 cut(s) 1304
AccB7I CCANNNNNTGG 1 cut(s) 1205
AccII CGCG 1 cut(s) 116
AciI CCGC 6 cut(s) 15, 29, 114, 335, 809, 989
AclWI GGATC 3 cut(s) 1106, 1294, 1307
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 4 cut(s) 511, 557, 803, 1269
AcuI CTGAAG 2 cut(s) 260, 1765
AcvI CACGTG 1 cut(s) 1070
AcyI GRCGYC 1 cut(s) 1596
AfaI GTAC 2 cut(s) 151, 490
AfiI CCNNNNNNNGG 2 cut(s) 1205, 1375
AflIII ACRYGT 2 cut(s) 742, 1480
AgsI TTSAA 7 cut(s) 359, 543, 740, 1085, 1147, 1254, 1268
AjnI CCWGG 3 cut(s) 636, 1368, 1696
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AleI CACNNNNGTG 1 cut(s) 1769
AluBI AGCT 6 cut(s) 467, 611, 816, 1142, 1444, 1610
AluI AGCT 6 cut(s) 467, 611, 816, 1142, 1444, 1610
Alw21I GWGCWC 1 cut(s) 1446
Alw26I GTCTC 2 cut(s) 438, 1431
AlwI GGATC 3 cut(s) 1106, 1294, 1307
AlwNI CAGNNNCTG 2 cut(s) 226, 1697
Ama87I CYCGRG 1 cut(s) 1304
AoxI GGCC 2 cut(s) 30, 204
ApeKI GCWGC 8 cut(s) 17, 20, 170, 611, 614, 878, 1607, 1734
ApoI RAATTY 4 cut(s) 511, 557, 803, 1269
Asp700I GAANNNNTTC 2 cut(s) 1272, 1333
AspS9I GGNCC 1 cut(s) 593
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 5 cut(s) 103, 224, 520, 869, 1220
AsuNHI GCTAGC 1 cut(s) 1616
AvaI CYCGRG 1 cut(s) 1304
AvaII GGWCC 1 cut(s) 593
AxyI CCTNAGG 1 cut(s) 208
BamHI GGATCC 1 cut(s) 1299
BanII GRGCYC 1 cut(s) 1446
BbrPI CACGTG 1 cut(s) 1070
BbsI GAAGAC 2 cut(s) 87, 663
Bbv12I GWGCWC 1 cut(s) 1446
BbvCI CCTCAGC 1 cut(s) 196
BbvI GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
BccI CCATC 4 cut(s) 383, 560, 1199, 1709
BceAI ACGGC 1 cut(s) 1637
BciT130I CCWGG 3 cut(s) 638, 1370, 1698
BciVI GTATCC 1 cut(s) 440
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 438, 1431
BfaI CTAG 4 cut(s) 438, 674, 813, 1617
BfmI CTRYAG 1 cut(s) 1725
BfuI GTATCC 1 cut(s) 440
Bme1390I CCNGG 4 cut(s) 377, 638, 1370, 1698
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 1304
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 1301
BmrFI CCNGG 4 cut(s) 377, 638, 1370, 1698
BmsI GCATC 9 cut(s) 326, 372, 970, 976, 1073, 1441, 1612, 1639, 1711
BmtI GCTAGC 1 cut(s) 1620
BpiI GAAGAC 2 cut(s) 87, 663
Bpu10I CCTNAGC 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 1070
BsaHI GRCGYC 1 cut(s) 1596
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 4 cut(s) 165, 201, 1368, 1653
BsaWI WCCGGW 1 cut(s) 305
BsaXI ACNNNNNCTCC 4 cut(s) 1581, 1611, 1668, 1698
Bsc4I CCNNNNNNNGG 2 cut(s) 1205, 1375
Bse1I ACTGG 2 cut(s) 1115, 1402
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 2 cut(s) 924, 1498
BseBI CCWGG 3 cut(s) 638, 1370, 1698
BseDI CCNNGG 4 cut(s) 165, 201, 1368, 1653
BseGI GGATG 4 cut(s) 571, 703, 1224, 1384
BseLI CCNNNNNNNGG 2 cut(s) 1205, 1375
BseMI GCAATG 2 cut(s) 924, 1498
BseMII CTCAG 7 cut(s) 142, 210, 222, 897, 1286, 1459, 1568
BseNI ACTGG 2 cut(s) 1115, 1402
BseXI GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
BsgI GTGCAG 2 cut(s) 651, 1626
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 2 cut(s) 32, 206
BsiHKAI GWGCWC 1 cut(s) 1446
BsiHKCI CYCGRG 1 cut(s) 1304
BsiSI CCGG 2 cut(s) 306, 377
BslFI GGGAC 2 cut(s) 828, 1374
BslI CCNNNNNNNGG 2 cut(s) 1205, 1375
BsmAI GTCTC 2 cut(s) 438, 1431
BsmFI GGGAC 2 cut(s) 828, 1374
BsnI GGCC 2 cut(s) 32, 206
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 1304
Bsp1286I GDGCHC 1 cut(s) 1446
Bsp143I GATC 3 cut(s) 700, 1111, 1299
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 6 cut(s) 15, 29, 114, 335, 809, 989
BspANI GGCC 2 cut(s) 32, 206
BspCNI CTCAG 7 cut(s) 141, 209, 221, 898, 1287, 1458, 1569
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 1301
BspOI GCTAGC 1 cut(s) 1620
BspPI GGATC 3 cut(s) 1106, 1294, 1307
BspTNI GGTCTC 1 cut(s) 438
BsrDI GCAATG 2 cut(s) 924, 1498
BsrI ACTGG 2 cut(s) 1115, 1402
BssECI CCNNGG 4 cut(s) 165, 201, 1368, 1653
BssMI GATC 3 cut(s) 700, 1111, 1299
BssNI GRCGYC 1 cut(s) 1596
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 638, 1370, 1698
Bst4CI ACNGT 3 cut(s) 823, 1520, 1553
Bst6I CTCTTC 1 cut(s) 436
BstACI GRCGYC 1 cut(s) 1596
BstAPI GCANNNNNTGC 1 cut(s) 1460
BstBAI YACGTR 1 cut(s) 1070
BstC8I GCNNGC 3 cut(s) 222, 1098, 1618
BstDSI CCRYGG 2 cut(s) 165, 1653
BstF5I GGATG 4 cut(s) 571, 703, 1224, 1384
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 3 cut(s) 703, 1114, 1302
BstMAI GTCTC 2 cut(s) 438, 1431
BstMBI GATC 3 cut(s) 700, 1111, 1299
BstNI CCWGG 3 cut(s) 638, 1370, 1698
BstNSI RCATGY 3 cut(s) 746, 1484, 1668
BstSCI CCNGG 4 cut(s) 375, 636, 1368, 1696
BstSFI CTRYAG 1 cut(s) 1725
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
BstV2I GAAGAC 2 cut(s) 87, 663
BstX2I RGATCY 2 cut(s) 1111, 1299
BstXI CCANNNNNNTGG 2 cut(s) 947, 1641
BstYI RGATCY 2 cut(s) 1111, 1299
Bsu36I CCTNAGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 2 cut(s) 32, 206
BtgI CCRYGG 2 cut(s) 165, 1653
BtgZI GCGATG 1 cut(s) 1635
BtsCI GGATG 4 cut(s) 571, 703, 1224, 1384
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 4 cut(s) 668, 1122, 1453, 1574
Cac8I GCNNGC 3 cut(s) 222, 1098, 1618
CaiI CAGNNNCTG 2 cut(s) 226, 1697
Cfr13I GGNCC 1 cut(s) 593
CseI GACGC 2 cut(s) 105, 1462
CsiI ACCWGGT 1 cut(s) 1696
Csp6I GTAC 2 cut(s) 150, 489
CviQI GTAC 2 cut(s) 150, 489
DpnI GATC 3 cut(s) 702, 1113, 1301
DpnII GATC 3 cut(s) 700, 1111, 1299
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 1444
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 1446
Eco31I GGTCTC 1 cut(s) 438
Eco32I GATATC 2 cut(s) 231, 1399
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 2 cut(s) 260, 1765
Eco72I CACGTG 1 cut(s) 1070
Eco81I CCTNAGG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 1304
EcoICRI GAGCTC 1 cut(s) 1444
EcoRII CCWGG 3 cut(s) 636, 1368, 1696
EcoRV GATATC 2 cut(s) 231, 1399
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 1446
ErhI CCWWGG 1 cut(s) 165
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FaqI GGGAC 2 cut(s) 828, 1374
FauI CCCGC 2 cut(s) 328, 816
FbaI TGATCA 1 cut(s) 700
FokI GGATG 4 cut(s) 578, 710, 1231, 1391
FriOI GRGCYC 1 cut(s) 1446
FspBI CTAG 4 cut(s) 438, 674, 813, 1617
HaeIII GGCC 2 cut(s) 32, 206
HapII CCGG 2 cut(s) 306, 377
HgaI GACGC 2 cut(s) 105, 1462
Hin1I GRCGYC 1 cut(s) 1596
HinfI GANTC 7 cut(s) 570, 622, 650, 758, 898, 1291, 1373
HpaII CCGG 2 cut(s) 306, 377
HphI GGTGA 5 cut(s) 103, 224, 520, 869, 1220
Hpy166II GTNNAC 1 cut(s) 1541
Hpy188III TCNNGA 6 cut(s) 130, 254, 400, 574, 1265, 1556
Hpy8I GTNNAC 1 cut(s) 1541
Hpy99I CGWCG 1 cut(s) 1557
HpyAV CCTTC 4 cut(s) 724, 857, 946, 1636
HpyCH4III ACNGT 3 cut(s) 823, 1520, 1553
HpyCH4IV ACGT 2 cut(s) 1069, 1596
HpySE526I ACGT 2 cut(s) 1069, 1596
Hsp92I GRCGYC 1 cut(s) 1596
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 3 cut(s) 700, 1111, 1299
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 8 cut(s) 4, 7, 157, 598, 601, 865, 1619, 1721
LweI GCATC 9 cut(s) 326, 372, 970, 976, 1073, 1441, 1612, 1639, 1711
MabI ACCWGGT 1 cut(s) 1696
MaeI CTAG 4 cut(s) 438, 674, 813, 1617
MaeII ACGT 2 cut(s) 1069, 1596
MaeIII GTNAC 1 cut(s) 346
MalI GATC 3 cut(s) 702, 1113, 1301
MboI GATC 3 cut(s) 700, 1111, 1299
MfeI CAATTG 1 cut(s) 1497
MflI RGATCY 2 cut(s) 1111, 1299
MhlI GDGCHC 1 cut(s) 1446
MlyI GAGTC 3 cut(s) 907, 1285, 1367
MmeI TCCRAC 1 cut(s) 964
MroXI GAANNNNTTC 2 cut(s) 1272, 1333
MseI TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
MslI CAYNNNNRTG 3 cut(s) 630, 1468, 1769
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 306, 377
MspR9I CCNGG 4 cut(s) 377, 638, 1370, 1698
MunI CAATTG 1 cut(s) 1497
MvaI CCWGG 3 cut(s) 638, 1370, 1698
MvnI CGCG 1 cut(s) 116
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 165
NdeII GATC 3 cut(s) 700, 1111, 1299
NheI GCTAGC 1 cut(s) 1616
NlaIV GGNNCC 1 cut(s) 1301
NmeAIII GCCGAG 1 cut(s) 182
NspI RCATGY 3 cut(s) 746, 1484, 1668
OliI CACNNNNGTG 1 cut(s) 1769
PaeR7I CTCGAG 1 cut(s) 1304
PciI ACATGT 2 cut(s) 742, 1480
PdmI GAANNNNTTC 2 cut(s) 1272, 1333
PfeI GAWTC 4 cut(s) 570, 622, 650, 758
PflMI CCANNNNNTGG 1 cut(s) 1205
PfoI TCCNGGA 1 cut(s) 375
PleI GAGTC 3 cut(s) 906, 1285, 1367
PmaCI CACGTG 1 cut(s) 1070
PmlI CACGTG 1 cut(s) 1070
PpsI GAGTC 3 cut(s) 906, 1285, 1367
Ppu21I YACGTR 1 cut(s) 1070
PscI ACATGT 2 cut(s) 742, 1480
Psp124BI GAGCTC 1 cut(s) 1446
Psp6I CCWGG 3 cut(s) 636, 1368, 1696
PspCI CACGTG 1 cut(s) 1070
PspGI CCWGG 3 cut(s) 636, 1368, 1696
PspN4I GGNNCC 1 cut(s) 1301
PspPI GGNCC 1 cut(s) 593
PspXI VCTCGAGB 1 cut(s) 1304
PstNI CAGNNNCTG 2 cut(s) 226, 1697
PsuI RGATCY 2 cut(s) 1111, 1299
RsaI GTAC 2 cut(s) 151, 490
RsaNI GTAC 2 cut(s) 150, 489
RseI CAYNNNNRTG 3 cut(s) 630, 1468, 1769
SacI GAGCTC 1 cut(s) 1446
SaqAI TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
Sau3AI GATC 3 cut(s) 700, 1111, 1299
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 3 cut(s) 907, 1285, 1367
ScrFI CCNGG 4 cut(s) 377, 638, 1370, 1698
SduI GDGCHC 1 cut(s) 1446
SexAI ACCWGGT 1 cut(s) 1696
SfaNI GCATC 9 cut(s) 326, 372, 970, 976, 1073, 1441, 1612, 1639, 1711
SfcI CTRYAG 1 cut(s) 1725
Sfr274I CTCGAG 1 cut(s) 1304
SinI GGWCC 1 cut(s) 593
SlaI CTCGAG 1 cut(s) 1304
SmiMI CAYNNNNRTG 3 cut(s) 630, 1468, 1769
SmlI CTYRAG 2 cut(s) 183, 1304
SmoI CTYRAG 2 cut(s) 183, 1304
SsiI CCGC 6 cut(s) 15, 29, 114, 335, 809, 989
SspMI CTAG 4 cut(s) 438, 674, 813, 1617
SstI GAGCTC 1 cut(s) 1446
StyD4I CCNGG 4 cut(s) 375, 636, 1368, 1696
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 3 cut(s) 823, 1520, 1553
TaiI ACGT 2 cut(s) 1072, 1599
TaqI TCGA 4 cut(s) 387, 495, 573, 1305
TatI WGTACW 1 cut(s) 149
TauI GCSGC 2 cut(s) 32, 992
TfiI GAWTC 4 cut(s) 570, 622, 650, 758
Tru1I TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
Tru9I TTAA 8 cut(s) 471, 684, 818, 912, 1385, 1407, 1565, 1789
TscAI CASTG 4 cut(s) 675, 1122, 1453, 1581
TseI GCWGC 8 cut(s) 17, 20, 170, 611, 614, 878, 1607, 1734
TspDTI ATGAA 1 cut(s) 410
TspRI CASTG 4 cut(s) 675, 1122, 1453, 1581
Van91I CCANNNNNTGG 1 cut(s) 1205
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 4 cut(s) 511, 557, 803, 1269
XceI RCATGY 3 cut(s) 746, 1484, 1668
XhoI CTCGAG 1 cut(s) 1304
XmnI GAANNNNTTC 2 cut(s) 1272, 1333
XspI CTAG 4 cut(s) 438, 674, 813, 1617
ZraI GACGTC 1 cut(s) 1597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.