AT2G35075

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004425
Physical Location & Seq
Forward (+)
14786460 .. 14789104
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35075.1

Sequence Viewer

Length: 1272 bp
ATGGGTATCAATGAGTTTAGCTCCTTTGGTCGCAGAGGAAGCGGCTTTCAGGAGCACAACGGTGAATCGCCGCCTTTTCCATGGATCCCAGCTCTATGGGTTCTGATGATTGAGAGTTGCAGGCGTTTCGTACAAAGCATGCAGGCAAAAGCTTTTTCAGGTTCTTTGACTGCCGGTGGTAATCAGGCTAAAGTGCTTTGCAGGACTTGTTTTTGGATTTGCCCGCTATCAAGAAAGTTTCTTGAAATTATGTATCCAGTTGTGCCGAGGTTAAGTTGCGCGAGAGGTACTGTGTTAGAGCCAGTAAGTTTGGATGCTGGGCATGCTATTTCTCTGGATTTGGAAACTGCCGCCCTTAAGATGTCAGCAGATTTGAACAGAACTGAGATTCCTTCATCTGTAAGCAGCAAGGCTCTGTTATACGAGAGCTTAAAACCAAGCATCTGGTGGGTTTCATATGCTTTCGCAAATCTAATGAGTCCACCCTCTACTCATTCTCCTTTTCAAGCATTCTTGGTTAAGAGCTCCAACCTGGAAGGTTTCACTCTGACTGAAGATGAGGCTGAATCCGTGGAGTCTGTGGTTATGCTTCTTCAGAAGGATGATTTCAAGGATCTGAAAACGCTGGGCAAGATGGAAACTATCATACAATTACTGCAGACCATTCCGAACCAAGAGCATTGCAGCAGCAAGTGGTGCAATAGGAGAGTAGCAATACAATGTGTAGATTTCTCTAAGCAGACTATCATTAGGGATGTGTGGCGTTTATTGGATAGTATTGCTGTGTCTTTGGCTTCTCAACAAAAGAAGTCATCAGTATCTTTAAAGGAAGTTTCTCCTAGTTTGTCTTGGTTATCATCCTGTAACATTCCAATGCCTGGTCTGGCGATTGAAGCATCAAGATATGGAATTTTAAGCGTTCACAACAAAGTTCCTGAGATAGTACTACTATCTTGGCTAACCCAGACAATGGAAGCCGCATTATGGTTGACAGGGAATTTCCATGATGTTGCTGCCATTGTTAGCTCGAGTTTGTTTTCATCAAGAGTGAAAGAAATCCGTATTAGGTTGCAGGAACATCATGATCTCTTACTGGCTATGTTACCAGCTGTTGTTTTTGTTGCTGAAGTTCGACTGAGTGTAGTTCCGGAGGCAACACAAGCACATCGCAACAGTAGTTACACAGAAGTTTCCTTTCTAGAGTTAGCAACCTCAGTAAAAGCCTCTCCTCTACTCGGACGGCACTTGCTTTGCAAAGAACATTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006464 GO:0006468 GO:0006611 GO:0006629 GO:0006644 GO:0006650 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019439 GO:0019538 GO:0019637 GO:0030258 GO:0031323 GO:0031503 GO:0031974 GO:0031981 GO:0032204 GO:0033036 GO:0033043 GO:0033044 GO:0033554 GO:0034613 GO:0034641 GO:0034655 GO:0036211 GO:0042162 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046486 GO:0046488 GO:0046700 GO:0046777 GO:0046834 GO:0046854 GO:0046907 GO:0048519 GO:0048583 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051052 GO:0051128 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097159 GO:0140096 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901575 GO:2001020
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
FATC PI3_PI4_kinase SMG1 WD40

Protein Analysis

423

Amino Acids

47.09

Weight (kDa)

7.6

Isoelectric Point (pI)

55.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 878, 970
AccII CGCG 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 1147
AciI CCGC 5 cut(s) 42, 71, 224, 351, 978
AclWI GGATC 3 cut(s) 79, 92, 621
AcsI RAATTY 2 cut(s) 909, 997
AcuI CTGAAG 3 cut(s) 573, 578, 1146
AfaI GTAC 3 cut(s) 132, 289, 945
AfiI CCNNNNNNNGG 4 cut(s) 878, 970, 984, 1235
AflII CTTAAG 1 cut(s) 356
AgsI TTSAA 5 cut(s) 245, 376, 506, 610, 893
AjnI CCWGG 2 cut(s) 531, 877
AleI CACNNNNGTG 1 cut(s) 60
AloI GAACNNNNNNTCC 2 cut(s) 373, 405
AluBI AGCT 7 cut(s) 21, 92, 152, 429, 525, 1026, 1109
AluI AGCT 7 cut(s) 21, 92, 152, 429, 525, 1026, 1109
Alw21I GWGCWC 2 cut(s) 57, 527
AlwI GGATC 3 cut(s) 79, 92, 621
Ama87I CYCGRG 1 cut(s) 1027
Aor13HI TCCGGA 1 cut(s) 1147
ApeKI GCWGC 4 cut(s) 405, 684, 687, 1013
ApoI RAATTY 2 cut(s) 909, 997
AspLEI GCGC 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 1027
BamHI GGATCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 57, 527
BbvI GCAGC 4 cut(s) 417, 696, 699, 1000
BccI CCATC 1 cut(s) 628
BceAI ACGGC 1 cut(s) 1256
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 2 cut(s) 533, 879
BciVI GTATCC 1 cut(s) 264
BfaI CTAG 2 cut(s) 840, 1199
BfmI CTRYAG 1 cut(s) 656
BfrI CTTAAG 1 cut(s) 356
BfuI GTATCC 1 cut(s) 264
BisI GCNGC 8 cut(s) 43, 71, 351, 406, 685, 688, 978, 1014
BlsI GCNGC 8 cut(s) 44, 72, 352, 407, 686, 689, 979, 1015
BmcAI AGTACT 1 cut(s) 945
Bme1390I CCNGG 2 cut(s) 533, 879
BmeT110I CYCGRG 1 cut(s) 1027
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 2 cut(s) 533, 879
BmsI GCATC 3 cut(s) 304, 450, 905
BplI GAGNNNNNCTC 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 80, 266, 570
BsaWI WCCGGW 1 cut(s) 1147
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 4 cut(s) 878, 970, 984, 1235
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 3 cut(s) 257, 302, 1098
Bse3DI GCAATG 1 cut(s) 679
BseAI TCCGGA 1 cut(s) 1147
BseBI CCWGG 2 cut(s) 533, 879
BseDI CCNNGG 3 cut(s) 80, 266, 570
BseGI GGATG 4 cut(s) 319, 607, 760, 857
BseLI CCNNNNNNNGG 4 cut(s) 878, 970, 984, 1235
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 4 cut(s) 375, 927, 1127, 1227
BseNI ACTGG 3 cut(s) 257, 302, 1098
BseRI GAGGAG 1 cut(s) 1218
BseXI GCAGC 4 cut(s) 417, 696, 699, 1000
BseYI CCCAGC 3 cut(s) 88, 317, 625
Bsh1236I CGCG 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 57, 527
BsiHKCI CYCGRG 1 cut(s) 1027
BsiSI CCGG 2 cut(s) 174, 1148
BslI CCNNNNNNNGG 4 cut(s) 878, 970, 984, 1235
BsmI GAATGC 1 cut(s) 509
BsoBI CYCGRG 1 cut(s) 1027
Bsp1286I GDGCHC 2 cut(s) 57, 527
Bsp13I TCCGGA 1 cut(s) 1147
Bsp143I GATC 3 cut(s) 84, 613, 1084
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 5 cut(s) 42, 71, 224, 351, 978
BspCNI CTCAG 4 cut(s) 376, 928, 1128, 1226
BspEI TCCGGA 1 cut(s) 1147
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 1 cut(s) 86
BspMAI CTGCAG 1 cut(s) 660
BspPI GGATC 3 cut(s) 79, 92, 621
BspTI CTTAAG 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 3 cut(s) 257, 302, 1098
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 80, 266, 570
BssMI GATC 3 cut(s) 84, 613, 1084
BssT1I CCWWGG 1 cut(s) 80
Bst2UI CCWGG 2 cut(s) 533, 879
Bst4CI ACNGT 3 cut(s) 62, 292, 1175
BstAFI CTTAAG 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 696
BstC8I GCNNGC 5 cut(s) 122, 140, 144, 224, 324
BstDEI CTNAG 5 cut(s) 384, 735, 936, 1136, 1213
BstDSI CCRYGG 2 cut(s) 80, 570
BstF5I GGATG 4 cut(s) 319, 607, 760, 857
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 3 cut(s) 87, 616, 1087
BstMBI GATC 3 cut(s) 84, 613, 1084
BstMWI GCNNNNNNNGC 5 cut(s) 39, 323, 696, 893, 1160
BstNI CCWGG 2 cut(s) 533, 879
BstNSI RCATGY 2 cut(s) 142, 326
BstSCI CCNGG 2 cut(s) 531, 877
BstSFI CTRYAG 1 cut(s) 656
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 4 cut(s) 417, 696, 699, 1000
BstX2I RGATCY 2 cut(s) 84, 613
BstXI CCANNNNNNTGG 2 cut(s) 96, 444
BstYI RGATCY 2 cut(s) 84, 613
BsuI GTATCC 1 cut(s) 264
BtgI CCRYGG 2 cut(s) 80, 570
BtgZI GCGATG 1 cut(s) 1151
BtsCI GGATG 4 cut(s) 319, 607, 760, 857
Cac8I GCNNGC 5 cut(s) 122, 140, 144, 224, 324
CciI TCATGA 1 cut(s) 1081
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 3 cut(s) 131, 288, 944
CviAII CATG 5 cut(s) 81, 139, 323, 1004, 1082
CviQI GTAC 3 cut(s) 131, 288, 944
DdeI CTNAG 5 cut(s) 384, 735, 936, 1136, 1213
DpnI GATC 3 cut(s) 86, 615, 1086
DpnII GATC 3 cut(s) 84, 613, 1084
DraI TTTAAA 1 cut(s) 825
Ecl136II GAGCTC 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 527
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 3 cut(s) 573, 578, 1146
Eco88I CYCGRG 1 cut(s) 1027
EcoICRI GAGCTC 1 cut(s) 525
EcoRII CCWGG 2 cut(s) 531, 877
EcoT14I CCWWGG 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 5 cut(s) 84, 142, 326, 1007, 1085
FatI CATG 5 cut(s) 80, 138, 322, 1003, 1081
FauI CCCGC 1 cut(s) 231
FauNDI CATATG 1 cut(s) 457
Fnu4HI GCNGC 8 cut(s) 43, 71, 351, 406, 685, 688, 978, 1014
FokI GGATG 4 cut(s) 326, 614, 767, 844
FriOI GRGCYC 1 cut(s) 527
Fsp4HI GCNGC 8 cut(s) 43, 71, 351, 406, 685, 688, 978, 1014
FspBI CTAG 2 cut(s) 840, 1199
GlaI GCGC 1 cut(s) 280
GluI GCNGC 8 cut(s) 43, 71, 351, 406, 685, 688, 978, 1014
GsaI CCCAGC 3 cut(s) 92, 321, 629
HapII CCGG 2 cut(s) 174, 1148
HhaI GCGC 1 cut(s) 281
Hin1II CATG 5 cut(s) 84, 142, 326, 1007, 1085
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 5 cut(s) 65, 388, 478, 566, 575
HpaII CCGG 2 cut(s) 174, 1148
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 3 cut(s) 482, 922, 990
Hpy188I TCNGA 7 cut(s) 105, 549, 597, 618, 669, 1238, 1271
Hpy8I GTNNAC 3 cut(s) 482, 922, 990
HpyAV CCTTC 3 cut(s) 402, 530, 592
HpyCH4III ACNGT 3 cut(s) 62, 292, 1175
HpyCH4V TGCA 8 cut(s) 120, 142, 201, 658, 684, 699, 1072, 1254
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 323, 696, 893, 1160
HpyF3I CTNAG 5 cut(s) 384, 735, 936, 1136, 1213
Hsp92II CATG 5 cut(s) 84, 142, 326, 1007, 1085
HspAI GCGC 1 cut(s) 279
Kpn2I TCCGGA 1 cut(s) 1147
Kzo9I GATC 3 cut(s) 84, 613, 1084
LmnI GCTCC 3 cut(s) 26, 52, 530
Lsp1109I GCAGC 4 cut(s) 417, 696, 699, 1000
LweI GCATC 3 cut(s) 304, 450, 905
MaeI CTAG 2 cut(s) 840, 1199
MaeIII GTNAC 3 cut(s) 863, 1101, 1178
MalI GATC 3 cut(s) 86, 615, 1086
MboI GATC 3 cut(s) 84, 613, 1084
MboII GAAGA 2 cut(s) 566, 584
MflI RGATCY 2 cut(s) 84, 613
MhlI GDGCHC 2 cut(s) 57, 527
MluCI AATT 4 cut(s) 246, 650, 909, 997
MlyI GAGTC 2 cut(s) 487, 584
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 9 cut(s) 29, 261, 278, 496, 553, 1144, 1222, 1234, 1239
MroI TCCGGA 1 cut(s) 1147
MseI TTAA 6 cut(s) 272, 357, 431, 519, 824, 914
MslI CAYNNNNRTG 2 cut(s) 60, 872
MspA1I CMGCKG 1 cut(s) 1109
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 2 cut(s) 174, 1148
MspR9I CCNGG 2 cut(s) 533, 879
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 2 cut(s) 533, 879
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 5 cut(s) 39, 323, 696, 893, 1160
NcoI CCATGG 1 cut(s) 80
NdeI CATATG 1 cut(s) 457
NdeII GATC 3 cut(s) 84, 613, 1084
NlaIII CATG 5 cut(s) 84, 142, 326, 1007, 1085
NlaIV GGNNCC 1 cut(s) 86
NmeAIII GCCGAG 1 cut(s) 291
NspI RCATGY 2 cut(s) 142, 326
OliI CACNNNNGTG 1 cut(s) 60
PaeI GCATGC 2 cut(s) 142, 326
PaeR7I CTCGAG 1 cut(s) 1027
PagI TCATGA 1 cut(s) 1081
PctI GAATGC 1 cut(s) 509
PfeI GAWTC 3 cut(s) 65, 388, 566
PflMI CCANNNNNTGG 2 cut(s) 878, 970
PkrI GCNGC 8 cut(s) 44, 72, 352, 407, 686, 689, 979, 1015
PleI GAGTC 2 cut(s) 486, 583
PpsI GAGTC 2 cut(s) 486, 583
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 531, 877
PspFI CCCAGC 3 cut(s) 88, 317, 625
PspGI CCWGG 2 cut(s) 531, 877
PspN4I GGNNCC 1 cut(s) 86
PspXI VCTCGAGB 1 cut(s) 1027
PstI CTGCAG 1 cut(s) 660
PsuI RGATCY 2 cut(s) 84, 613
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 3 cut(s) 132, 289, 945
RsaNI GTAC 3 cut(s) 131, 288, 944
RseI CAYNNNNRTG 2 cut(s) 60, 872
SacI GAGCTC 1 cut(s) 527
SaqAI TTAA 6 cut(s) 272, 357, 431, 519, 824, 914
SatI GCNGC 8 cut(s) 43, 71, 351, 406, 685, 688, 978, 1014
Sau3AI GATC 3 cut(s) 84, 613, 1084
ScaI AGTACT 1 cut(s) 945
SchI GAGTC 2 cut(s) 487, 584
ScrFI CCNGG 2 cut(s) 533, 879
SduI GDGCHC 2 cut(s) 57, 527
SfaNI GCATC 3 cut(s) 304, 450, 905
SfcI CTRYAG 1 cut(s) 656
Sfr274I CTCGAG 1 cut(s) 1027
SlaI CTCGAG 1 cut(s) 1027
SmiMI CAYNNNNRTG 2 cut(s) 60, 872
SmlI CTYRAG 2 cut(s) 356, 1027
SmoI CTYRAG 2 cut(s) 356, 1027
SphI GCATGC 2 cut(s) 142, 326
Sse9I AATT 4 cut(s) 246, 650, 909, 997
SsiI CCGC 5 cut(s) 42, 71, 224, 351, 978
SspMI CTAG 2 cut(s) 840, 1199
SstI GAGCTC 1 cut(s) 527
StyD4I CCNGG 2 cut(s) 531, 877
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 3 cut(s) 62, 292, 1175
TaqI TCGA 2 cut(s) 1028, 1132
TasI AATT 4 cut(s) 246, 650, 909, 997
TatI WGTACW 1 cut(s) 943
TauI GCSGC 4 cut(s) 45, 73, 353, 980
TfiI GAWTC 3 cut(s) 65, 388, 566
Tru1I TTAA 6 cut(s) 272, 357, 431, 519, 824, 914
Tru9I TTAA 6 cut(s) 272, 357, 431, 519, 824, 914
TseI GCWGC 4 cut(s) 405, 684, 687, 1013
TspDTI ATGAA 3 cut(s) 384, 444, 1029
TspGWI ACGGA 2 cut(s) 559, 1049
Van91I CCANNNNNTGG 2 cut(s) 878, 970
Vha464I CTTAAG 1 cut(s) 356
XapI RAATTY 2 cut(s) 909, 997
XbaI TCTAGA 1 cut(s) 1198
XceI RCATGY 2 cut(s) 142, 326
XcmI CCANNNNNNNNNTGG 1 cut(s) 444
XhoI CTCGAG 1 cut(s) 1027
XspI CTAG 2 cut(s) 840, 1199
ZrmI AGTACT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.