Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004425

Physical Location & Seq

Genomic Coordinates Forward (+)

27287735 .. 27292959

Gene Structure 5225 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1180200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGATTAAAGTTTTGGTGTATGTGCAGGAAGGGATAAGGTTTTTGAATACATGGTTAGAAGGAGAAAGATTGATTGGTTTCTGTAAATATCTCGGAAAGAACACTCCAGAGCTTAAACCAGATGAAATTCCTCACTGTATGATGATGATTTTATTCAAGATTAATTGCAAAACCTGGCCATTCTTTATTAATCTGCTGATTCAGTGTGCTTCGTTGGAGATATCCTCCTCAAGCAAGCGGCGATTGCCCAAGTTGATATATGCAAAGACTCTACGGATAGTTGTGCAACAAGCAGAGGATGATAAAATTTTGAATGATGCAAACATGGTACATTCTTATGATGGTCTGCTATGGACACTGAGCTTGCAGGAACTTTCTTCTGTGCTGTTGCTTCCAGATCCAAAGCTGAAGAAACTGTCAGACTCGTCTCCCTCGAATGAGTTGTCTTGGCTGAACATGGAATGGAGTTCCATTTTGGAACGGACACAGTTACACATGAATTTACTGGAGCCTTTTATAGTGTTCAGGCGAGTGCTACTTCAAATCTCAAGTTGTAGGGACTGTGCAACATCTTCTGCAGGTTCTAGATTTTCTCAAGCCGCTGCAGCCTTGCATGAGTTTAAGTTGCTCGCTGTCGAATCAGGATTGGAAGGGTACAGACTTGAAGAAGCAAAGCTTCTGCGTGGCCAAGGTCAGCATGAGATGGCCTTCAATGGAGTAATGCAACAATTTTTTGGTTTAGTCAACACCTTTCTACAGAACCACCAGGATACATGGAAAAGAAGATTAGGAGTGCGTACATACAAGGTAGTTCCATTTACTCCAAGTGCCGGTGTTCTTGAATGGGTTAATGGCAGTCTTCCTCTTGGAGAATATCTTATAGGAAGGCCTGTCATGCATCATTTTTTCTTGGAGAGGTTTTTGCAACCGGCTGACTGGTTTGAGAAGAGACTTTCATACACGCGAAGTGTTGCTGCTAGTTCAATGGTTGGTTATATTGTTGGCCTCGGAGATCGACATTCTATGAATATCTTAATTGATCAAATGACTGCAGAAGTTGTTCGTATAGATCTTGGCGTTGCCTTTGAACAAGGCTTGATGCTCAAAACACCAGAACGGGTTTGTACTTTACTTACAAGGGACATCATTGATGGCATGGGTGTCACCGGAGTGGAAGGGTTTTTCAGAAGATGCTGTGAAGAAACTCTTTCTGTTATGAGGACAAATAAAGAAGCACTACTGACCATCGTTGAGGAAACGGATGATGACTTGAATTTAAGCTTTGAAGGCTCGCAAGATGAATATGAAGGTAACAAGGATGCTGCACGTGCACTGATGCGTGTAAAACAAAACTCGATGGATATGAAGAAGGGAAATGCGAAGCATACATGGACAGGTAGTTCTCGTGCCCCGCAGTTTCATCAATGTACCTATAATATGGAACAAGTTCAACTACTTATACAAGATGCGATTGATCCCGAGCGTCTTTGTCAACTGTTTCCTGGATGGGGAGCGTGGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko01524 ko03440 ko04064 ko04068 ko04110 ko04115 ko04210 ko04214 ko04218 ko05165 ko05166 ko05202 ko05206 map01524 map03440 map04064 map04068 map04110 map04115 map04210 map04214 map04218 map05165 map05166 map05202 map05206

Pfam Domains

Protein Families

FAT FATC PI3_PI4_kinase PWWP

Protein Analysis

508

Amino Acids

57.87

Weight (kDa)

6.3

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI3_PI4_kinase	PF00454	298 - 419	3.5e-29	Phosphatidylinositol 3- and 4-kinase
FATC	PF02260	481 - 507	1.2e-10	FATC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35075
fragaria_vesca	FvH4_2g07370 FvH4_2g07370
malus_domestica	MD10G1086500.v1.1 MD14G1180200.v1.1
prunus_persica	Prupe.8G112500_v2.0.a1
pyrus_communis	pycom10g06870
rosa_chinensis	RchiOBHm_Chr6g0260251 RchiOBHm_Chr6g0260261 RchiOBHm_Chr6g0260791
rosa_laevigata	RLG00000014485
rosa_multiflora	Rmu_co8055024.1_g000001 Rmu_co8166074.1_g000001 Rmu_sc0014629.1_g000010 Rmu_sc0023276.1_g000001
rosa_roxburghii	Rroxscaffold_7G00207390
rosa_rugosa	Rorug05G0592900
rosa_samantha	Rh6AG109300 Rh6AG110500 Rh6AG112300 Rh6BG106200 Rh6CG097700 Rh6CG098100 Rh6CG102000 Rh6CG102200 Rh6CG103000 Rh6DG091700 Rh6DG093600 Rh6DG093900 Rh6DG094300
rosa_wichuraiana	Rw6G009500 Rw6G009940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	569
AccII	CGCG	1 cut(s)	964
AciI	CCGC	3 cut(s)	238, 600, 1412
AclWI	GGATC	2 cut(s)	392, 1469
AcoI	YGGCCR	2 cut(s)	176, 685
AcsI	RAATTY	4 cut(s)	126, 306, 499, 1273
AcuI	CTGAAG	1 cut(s)	428
AcvI	CACGTG	1 cut(s)	1328
AfaI	GTAC	5 cut(s)	330, 656, 799, 1126, 1429
AfiI	CCNNNNNNNGG	2 cut(s)	830, 1508
AjnI	CCWGG	3 cut(s)	173, 765, 1501
AjuI	GAANNNNNNNTTGG	2 cut(s)	57, 89
AleI	CACNNNNGTG	1 cut(s)	1169
AloI	GAACNNNNNNTCC	2 cut(s)	1384, 1416
AluBI	AGCT	5 cut(s)	112, 363, 406, 676, 1281
AluI	AGCT	5 cut(s)	112, 363, 406, 676, 1281
Alw21I	GWGCWC	1 cut(s)	1333
Alw26I	GTCTC	2 cut(s)	432, 943
Alw44I	GTGCAC	1 cut(s)	1329
AlwI	GGATC	2 cut(s)	392, 1469
Ama87I	CYCGRG	1 cut(s)	1478
AoxI	GGCC	5 cut(s)	176, 685, 705, 887, 1003
ApaLI	GTGCAC	1 cut(s)	1329
ApeKI	GCWGC	4 cut(s)	602, 605, 974, 1322
ApoI	RAATTY	4 cut(s)	126, 306, 499, 1273
ArsI	GACNNNNNNTTYG	2 cut(s)	428, 460
AseI	ATTAAT	2 cut(s)	162, 189
Asp700I	GAANNNNTTC	2 cut(s)	1059, 1446
AsuHPI	GGTGA	1 cut(s)	1156
AvaI	CYCGRG	1 cut(s)	1478
BaeGI	GKGCMC	2 cut(s)	1333, 1411
BalI	TGGCCA	2 cut(s)	178, 687
BauI	CACGAG	1 cut(s)	1404
BbrPI	CACGTG	1 cut(s)	1328
BbsI	GAAGAC	1 cut(s)	851
Bbv12I	GWGCWC	1 cut(s)	1333
BbvI	GCAGC	4 cut(s)	589, 617, 961, 1309
BccI	CCATC	6 cut(s)	335, 697, 1145, 1253, 1351, 1500
BciT130I	CCWGG	3 cut(s)	175, 767, 1503
BciVI	GTATCC	1 cut(s)	763
BclI	TGATCA	1 cut(s)	1039
BcoDI	GTCTC	2 cut(s)	432, 943
BfaI	CTAG	2 cut(s)	585, 978
BfmI	CTRYAG	4 cut(s)	576, 603, 755, 1050
BfuAI	ACCTGC	1 cut(s)	569
BfuI	GTATCC	1 cut(s)	763
BglII	AGATCT	1 cut(s)	1069
BisI	GCNGC	6 cut(s)	239, 600, 603, 606, 975, 1323
BlsI	GCNGC	6 cut(s)	240, 601, 604, 607, 976, 1324
Bme1390I	CCNGG	3 cut(s)	175, 767, 1503
BmeT110I	CYCGRG	1 cut(s)	1478
BmiI	GGNNCC	1 cut(s)	510
BmrFI	CCNGG	3 cut(s)	175, 767, 1503
BmsI	GCATC	7 cut(s)	307, 907, 1089, 1181, 1309, 1326, 1456
BpiI	GAAGAC	1 cut(s)	851
BplI	GAGNNNNNCTC	2 cut(s)	209, 241
BpmI	CTGGAG	2 cut(s)	90, 527
BpuEI	CTTGAG	3 cut(s)	214, 532, 579
BsaAI	YACGTR	1 cut(s)	1328
BsaJI	CCNNGG	2 cut(s)	688, 1006
BsaWI	WCCGGW	1 cut(s)	1166
Bsc4I	CCNNNNNNNGG	2 cut(s)	830, 1508
Bse118I	RCCGGY	2 cut(s)	830, 928
Bse1I	ACTGG	2 cut(s)	510, 941
BseBI	CCWGG	3 cut(s)	175, 767, 1503
BseDI	CCNNGG	2 cut(s)	688, 1006
BseGI	GGATG	4 cut(s)	304, 1267, 1324, 1511
BseLI	CCNNNNNNNGG	2 cut(s)	830, 1508
BseMII	CTCAG	1 cut(s)	350
BseNI	ACTGG	2 cut(s)	510, 941
BseRI	GAGGAG	1 cut(s)	217
BseSI	GKGCMC	2 cut(s)	1333, 1411
BseXI	GCAGC	4 cut(s)	589, 617, 961, 1309
BsgI	GTGCAG	2 cut(s)	44, 1308
Bsh1236I	CGCG	1 cut(s)	964
BshFI	GGCC	5 cut(s)	178, 687, 707, 889, 1005
BsiHKAI	GWGCWC	1 cut(s)	1333
BsiHKCI	CYCGRG	1 cut(s)	1478
BsiSI	CCGG	3 cut(s)	831, 929, 1167
BslFI	GGGAC	2 cut(s)	572, 1154
BslI	CCNNNNNNNGG	2 cut(s)	830, 1508
BsmAI	GTCTC	2 cut(s)	432, 943
BsmBI	CGTCTC	1 cut(s)	432
BsmFI	GGGAC	2 cut(s)	572, 1154
BsnI	GGCC	5 cut(s)	178, 687, 707, 889, 1005
BsoBI	CYCGRG	1 cut(s)	1478
Bsp1286I	GDGCHC	2 cut(s)	1333, 1411
Bsp143I	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
BspACI	CCGC	3 cut(s)	238, 600, 1412
BspANI	GGCC	5 cut(s)	178, 687, 707, 889, 1005
BspCNI	CTCAG	1 cut(s)	351
BspFNI	CGCG	1 cut(s)	964
BspLI	GGNNCC	1 cut(s)	510
BspMAI	CTGCAG	3 cut(s)	580, 607, 1054
BspMI	ACCTGC	1 cut(s)	569
BspPI	GGATC	2 cut(s)	392, 1469
BsrFI	RCCGGY	2 cut(s)	830, 928
BsrI	ACTGG	2 cut(s)	510, 941
BssAI	RCCGGY	2 cut(s)	830, 928
BssECI	CCNNGG	2 cut(s)	688, 1006
BssMI	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
BssSI	CACGAG	1 cut(s)	1404
BssT1I	CCWWGG	1 cut(s)	688
Bst2BI	CACGAG	1 cut(s)	1404
Bst2UI	CCWGG	3 cut(s)	175, 767, 1503
Bst4CI	ACNGT	5 cut(s)	137, 417, 489, 563, 1497
Bst6I	CTCTTC	1 cut(s)	941
BstBAI	YACGTR	1 cut(s)	1328
BstC8I	GCNNGC	4 cut(s)	236, 365, 630, 1292
BstDEI	CTNAG	1 cut(s)	359
BstF5I	GGATG	4 cut(s)	304, 1267, 1324, 1511
BstFNI	CGCG	1 cut(s)	964
BstKTI	GATC	5 cut(s)	400, 1015, 1042, 1072, 1477
BstMAI	GTCTC	2 cut(s)	432, 943
BstMBI	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
BstMWI	GCNNNNNNNGC	5 cut(s)	244, 605, 895, 1287, 1328
BstNI	CCWGG	3 cut(s)	175, 767, 1503
BstSCI	CCNGG	3 cut(s)	173, 765, 1501
BstSFI	CTRYAG	4 cut(s)	576, 603, 755, 1050
BstSLI	GKGCMC	2 cut(s)	1333, 1411
BstUI	CGCG	1 cut(s)	964
BstV1I	GCAGC	4 cut(s)	589, 617, 961, 1309
BstV2I	GAAGAC	1 cut(s)	851
BstX2I	RGATCY	2 cut(s)	397, 1069
BstYI	RGATCY	2 cut(s)	397, 1069
BsuI	GTATCC	1 cut(s)	763
BsuRI	GGCC	5 cut(s)	178, 687, 707, 889, 1005
BtsCI	GGATG	4 cut(s)	304, 1267, 1324, 1511
BtsIMutI	CAGTG	4 cut(s)	133, 209, 356, 1331
BveI	ACCTGC	1 cut(s)	569
Cac8I	GCNNGC	4 cut(s)	236, 365, 630, 1292
Cfr10I	RCCGGY	2 cut(s)	830, 928
CseI	GACGC	1 cut(s)	1472
Csp6I	GTAC	5 cut(s)	329, 655, 798, 1125, 1428
CviQI	GTAC	5 cut(s)	329, 655, 798, 1125, 1428
DdeI	CTNAG	1 cut(s)	359
DpnI	GATC	5 cut(s)	399, 1014, 1041, 1071, 1476
DpnII	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
EaeI	YGGCCR	2 cut(s)	176, 685
Eam1104I	CTCTTC	1 cut(s)	941
EarI	CTCTTC	1 cut(s)	941
Eco130I	CCWWGG	1 cut(s)	688
Eco147I	AGGCCT	1 cut(s)	889
Eco32I	GATATC	1 cut(s)	222
Eco57I	CTGAAG	1 cut(s)	428
Eco72I	CACGTG	1 cut(s)	1328
Eco88I	CYCGRG	1 cut(s)	1478
EcoRII	CCWGG	3 cut(s)	173, 765, 1501
EcoRV	GATATC	1 cut(s)	222
EcoT14I	CCWWGG	1 cut(s)	688
EcoT22I	ATGCAT	1 cut(s)	900
ErhI	CCWWGG	1 cut(s)	688
Esp3I	CGTCTC	1 cut(s)	432
FalI	AAGNNNNNCTT	4 cut(s)	660, 692, 1191, 1223
FaqI	GGGAC	2 cut(s)	572, 1154
FauI	CCCGC	1 cut(s)	1419
FbaI	TGATCA	1 cut(s)	1039
Fnu4HI	GCNGC	6 cut(s)	239, 600, 603, 606, 975, 1323
FokI	GGATG	4 cut(s)	311, 1274, 1331, 1518
Fsp4HI	GCNGC	6 cut(s)	239, 600, 603, 606, 975, 1323
FspBI	CTAG	2 cut(s)	585, 978
GluI	GCNGC	6 cut(s)	239, 600, 603, 606, 975, 1323
GsuI	CTGGAG	2 cut(s)	90, 527
HaeIII	GGCC	5 cut(s)	178, 687, 707, 889, 1005
HapII	CCGG	3 cut(s)	831, 929, 1167
HgaI	GACGC	1 cut(s)	1472
HincII	GTYRAC	2 cut(s)	745, 1493
HindII	GTYRAC	2 cut(s)	745, 1493
HindIII	AAGCTT	2 cut(s)	674, 1279
HinfI	GANTC	4 cut(s)	199, 268, 422, 638
HpaII	CCGG	3 cut(s)	831, 929, 1167
HphI	GGTGA	1 cut(s)	1156
Hpy166II	GTNNAC	3 cut(s)	745, 1331, 1493
Hpy188I	TCNGA	4 cut(s)	95, 421, 1010, 1187
Hpy188III	TCNNGA	7 cut(s)	107, 157, 395, 585, 642, 839, 1478
Hpy8I	GTNNAC	3 cut(s)	745, 1331, 1493
HpyAV	CCTTC	9 cut(s)	23, 53, 644, 718, 879, 1169, 1280, 1301, 1363
HpyCH4III	ACNGT	5 cut(s)	137, 417, 489, 563, 1497
HpyCH4IV	ACGT	1 cut(s)	1327
HpyF10VI	GCNNNNNNNGC	5 cut(s)	244, 605, 895, 1287, 1328
HpyF3I	CTNAG	1 cut(s)	359
HpySE526I	ACGT	1 cut(s)	1327
Ksp22I	TGATCA	1 cut(s)	1039
Kzo9I	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
LmnI	GCTCC	2 cut(s)	508, 1511
Lsp1109I	GCAGC	4 cut(s)	589, 617, 961, 1309
LweI	GCATC	7 cut(s)	307, 907, 1089, 1181, 1309, 1326, 1456
MaeI	CTAG	2 cut(s)	585, 978
MaeII	ACGT	1 cut(s)	1327
MaeIII	GTNAC	3 cut(s)	489, 1162, 1310
MalI	GATC	5 cut(s)	399, 1014, 1041, 1071, 1476
MboI	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
MflI	RGATCY	2 cut(s)	397, 1069
MhlI	GDGCHC	2 cut(s)	1333, 1411
MlsI	TGGCCA	2 cut(s)	178, 687
MluCI	AATT	7 cut(s)	126, 163, 306, 499, 728, 1035, 1273
MluNI	TGGCCA	2 cut(s)	178, 687
MlyI	GAGTC	2 cut(s)	262, 416
MmeI	TCCRAC	1 cut(s)	195
Mox20I	TGGCCA	2 cut(s)	178, 687
Mph1103I	ATGCAT	1 cut(s)	900
MroXI	GAANNNNTTC	2 cut(s)	1059, 1446
MscI	TGGCCA	2 cut(s)	178, 687
MseI	TTAA	8 cut(s)	6, 114, 162, 189, 621, 849, 1034, 1277
MslI	CAYNNNNRTG	2 cut(s)	336, 1169
Msp20I	TGGCCA	2 cut(s)	178, 687
MspA1I	CMGCKG	1 cut(s)	602
MspI	CCGG	3 cut(s)	831, 929, 1167
MspR9I	CCNGG	3 cut(s)	175, 767, 1503
MvaI	CCWGG	3 cut(s)	175, 767, 1503
MvnI	CGCG	1 cut(s)	964
MwoI	GCNNNNNNNGC	5 cut(s)	244, 605, 895, 1287, 1328
NdeII	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
NlaIV	GGNNCC	1 cut(s)	510
NmuCI	GTSAC	1 cut(s)	1162
NsiI	ATGCAT	1 cut(s)	900
OliI	CACNNNNGTG	1 cut(s)	1169
PceI	AGGCCT	1 cut(s)	889
PcsI	WCGNNNNNNNCGW	1 cut(s)	431
PdmI	GAANNNNTTC	2 cut(s)	1059, 1446
PfeI	GAWTC	2 cut(s)	199, 638
PfoI	TCCNGGA	1 cut(s)	1501
PkrI	GCNGC	6 cut(s)	240, 601, 604, 607, 976, 1324
PleI	GAGTC	2 cut(s)	262, 416
PmaCI	CACGTG	1 cut(s)	1328
PmlI	CACGTG	1 cut(s)	1328
PpsI	GAGTC	2 cut(s)	262, 416
Ppu21I	YACGTR	1 cut(s)	1328
PshBI	ATTAAT	2 cut(s)	162, 189
Psp6I	CCWGG	3 cut(s)	173, 765, 1501
PspCI	CACGTG	1 cut(s)	1328
PspGI	CCWGG	3 cut(s)	173, 765, 1501
PspN4I	GGNNCC	1 cut(s)	510
PstI	CTGCAG	3 cut(s)	580, 607, 1054
PsuI	RGATCY	2 cut(s)	397, 1069
RsaI	GTAC	5 cut(s)	330, 656, 799, 1126, 1429
RsaNI	GTAC	5 cut(s)	329, 655, 798, 1125, 1428
RseI	CAYNNNNRTG	2 cut(s)	336, 1169
SaqAI	TTAA	8 cut(s)	6, 114, 162, 189, 621, 849, 1034, 1277
SatI	GCNGC	6 cut(s)	239, 600, 603, 606, 975, 1323
Sau3AI	GATC	5 cut(s)	397, 1012, 1039, 1069, 1474
SchI	GAGTC	2 cut(s)	262, 416
ScrFI	CCNGG	3 cut(s)	175, 767, 1503
SduI	GDGCHC	2 cut(s)	1333, 1411
SfaNI	GCATC	7 cut(s)	307, 907, 1089, 1181, 1309, 1326, 1456
SfcI	CTRYAG	4 cut(s)	576, 603, 755, 1050
SmiMI	CAYNNNNRTG	2 cut(s)	336, 1169
SmlI	CTYRAG	3 cut(s)	229, 547, 594
SmoI	CTYRAG	3 cut(s)	229, 547, 594
Sse9I	AATT	7 cut(s)	126, 163, 306, 499, 728, 1035, 1273
SseBI	AGGCCT	1 cut(s)	889
SsiI	CCGC	3 cut(s)	238, 600, 1412
SspMI	CTAG	2 cut(s)	585, 978
StuI	AGGCCT	1 cut(s)	889
StyD4I	CCNGG	3 cut(s)	173, 765, 1501
StyI	CCWWGG	1 cut(s)	688
TaaI	ACNGT	5 cut(s)	137, 417, 489, 563, 1497
TaiI	ACGT	1 cut(s)	1330
TaqI	TCGA	4 cut(s)	434, 636, 1015, 1355
TasI	AATT	7 cut(s)	126, 163, 306, 499, 728, 1035, 1273
TatI	WGTACW	1 cut(s)	1124
TauI	GCSGC	2 cut(s)	241, 602
TfiI	GAWTC	2 cut(s)	199, 638
Tru1I	TTAA	8 cut(s)	6, 114, 162, 189, 621, 849, 1034, 1277
Tru9I	TTAA	8 cut(s)	6, 114, 162, 189, 621, 849, 1034, 1277
TscAI	CASTG	4 cut(s)	140, 209, 363, 1338
TseFI	GTSAC	1 cut(s)	1162
TseI	GCWGC	4 cut(s)	602, 605, 974, 1322
Tsp45I	GTSAC	1 cut(s)	1162
TspDTI	ATGAA	8 cut(s)	138, 512, 945, 1040, 1314, 1320, 1379, 1409
TspGWI	ACGGA	3 cut(s)	289, 496, 1274
TspRI	CASTG	4 cut(s)	140, 209, 363, 1338
VneI	GTGCAC	1 cut(s)	1329
VspI	ATTAAT	2 cut(s)	162, 189
XapI	RAATTY	4 cut(s)	126, 306, 499, 1273
XbaI	TCTAGA	1 cut(s)	584
XmnI	GAANNNNTTC	2 cut(s)	1059, 1446
XspI	CTAG	2 cut(s)	585, 978
Zsp2I	ATGCAT	1 cut(s)	900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.