RchiOBHm_Chr6g0260251

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004425
Physical Location & Seq
Forward (+)
15331982 .. 15332626
645 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23338

Sequence Viewer

Length: 645 bp
ATGATGGCTCCTATTGTTGTGGCCTTAGAACATCGTCTGTCTTCCACATCTCGGAAACCTGAAACACCTCATGAGGTATGGTTCCATGGGGAGTATAAAGATCGGTTGAAATCAGCCATCATAGCCTTCAAGACACCTCCGGCATGTGCTACAGCACTTGGAGATGCTTGGCGTCCATTTGATACCATTGCTGCCTCCTTAGCATCTTATCAAAGGAAGTCATCAATTTGTTTAAGAGAAGTCGCACCACAATTGGCTCTACTATCATCATCTGATGTTCCAATGCCTGGTCTTGAAAAGCAAGATACAGTCTCTGAATCTGACAGAGGTCTCAGTGCTAATCTCCAAGGAATTGTTACCATTGCTTCTTTCGCTGCGGATGCGGCTATCATATCAACCAAGACTAAGCGTAAGAAACTTGTTTTTCTTGGTTCTGATGGTCAAAAGTACACATATCTTTTGAAAGGCAGGGAAGATCTCCGGTTTGATGCCAGAATCATGCAGCTCTTGCAAGCTATCAACTGTTTTCTGCATTCCTCAGTGGCAACTCATAGTCACTTTCTTGGTATTCGCTATTATTCTGTGACTCCAATTAGTGGCCGGGCTGGTCTTATCCAGTGGGTGGGTAATGTGATTAGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006401 GO:0006402 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019439 GO:0019538 GO:0019637 GO:0030258 GO:0031323 GO:0031974 GO:0031981 GO:0032204 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034655 GO:0036211 GO:0042162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046486 GO:0046488 GO:0046700 GO:0046777 GO:0046834 GO:0046854 GO:0048519 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097159 GO:0140096 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901575 GO:2001020
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
FATC PI3_PI4_kinase SMG1 WD40

Protein Analysis

214

Amino Acids

23.51

Weight (kDa)

9.43

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 150 - 211 6.9e-07 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 287, 596, 622
AciI CCGC 2 cut(s) 377, 383
AcoI YGGCCR 1 cut(s) 598
AcyI GRCGYC 1 cut(s) 172
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 4 cut(s) 51, 287, 596, 622
AgsI TTSAA 4 cut(s) 109, 130, 296, 463
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 2 cut(s) 505, 515
AluI AGCT 2 cut(s) 505, 515
Alw26I GTCTC 2 cut(s) 316, 335
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 2 cut(s) 21, 598
ApeKI GCWGC 3 cut(s) 191, 374, 502
AsuC2I CCSGG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 178, 361, 514
BccI CCATC 2 cut(s) 125, 431
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 2 cut(s) 316, 335
BfmI CTRYAG 1 cut(s) 150
BglII AGATCT 1 cut(s) 475
BisI GCNGC 4 cut(s) 192, 375, 384, 503
BlsI GCNGC 4 cut(s) 193, 376, 385, 504
Bme1390I CCNGG 2 cut(s) 288, 602
BmiI GGNNCC 2 cut(s) 9, 83
BmrFI CCNGG 2 cut(s) 288, 602
BmsI GCATC 4 cut(s) 154, 212, 370, 478
BoxI GACNNNNGTC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 199
BpuMI CCSGG 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 172
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 2 cut(s) 85, 346
BsaWI WCCGGW 1 cut(s) 480
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 287, 596, 622
Bse1I ACTGG 1 cut(s) 616
Bse3DI GCAATG 2 cut(s) 186, 360
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 85, 346
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 4 cut(s) 51, 287, 596, 622
BseMI GCAATG 2 cut(s) 186, 360
BseMII CTCAG 2 cut(s) 346, 552
BseNI ACTGG 1 cut(s) 616
BseXI GCAGC 3 cut(s) 178, 361, 514
BshFI GGCC 2 cut(s) 23, 600
BsiSI CCGG 3 cut(s) 140, 481, 601
BslI CCNNNNNNNGG 4 cut(s) 51, 287, 596, 622
BsmAI GTCTC 2 cut(s) 316, 335
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 23, 600
Bso31I GGTCTC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 100, 475
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 2 cut(s) 377, 383
BspANI GGCC 2 cut(s) 23, 600
BspCNI CTCAG 2 cut(s) 345, 551
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 9, 83
BspTNI GGTCTC 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 186, 360
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 2 cut(s) 85, 346
BssMI GATC 2 cut(s) 100, 475
BssNI GRCGYC 1 cut(s) 172
BssT1I CCWWGG 2 cut(s) 85, 346
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 310, 524
BstACI GRCGYC 1 cut(s) 172
BstAPI GCANNNNNTGC 1 cut(s) 508
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 5 cut(s) 25, 199, 332, 405, 538
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 1 cut(s) 385
BstKTI GATC 2 cut(s) 103, 478
BstMAI GTCTC 2 cut(s) 316, 335
BstMBI GATC 2 cut(s) 100, 475
BstMWI GCNNNNNNNGC 6 cut(s) 122, 200, 371, 380, 383, 508
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 1 cut(s) 147
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 2 cut(s) 286, 600
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 178, 361, 514
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BsuRI GGCC 2 cut(s) 23, 600
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 3 cut(s) 340, 546, 623
Cac8I GCNNGC 1 cut(s) 513
CaiI CAGNNNCTG 1 cut(s) 314
CciI TCATGA 1 cut(s) 70
CseI GACGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 448
CviAII CATG 4 cut(s) 71, 86, 144, 499
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 5 cut(s) 25, 199, 332, 405, 538
DpnI GATC 2 cut(s) 102, 477
DpnII GATC 2 cut(s) 100, 475
EaeI YGGCCR 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 85, 346
Eco31I GGTCTC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 85, 346
ErhI CCWWGG 2 cut(s) 85, 346
FaeI CATG 4 cut(s) 74, 89, 147, 502
FatI CATG 4 cut(s) 70, 85, 143, 498
Fnu4HI GCNGC 4 cut(s) 192, 375, 384, 503
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 4 cut(s) 192, 375, 384, 503
GluI GCNGC 4 cut(s) 192, 375, 384, 503
HaeIII GGCC 2 cut(s) 23, 600
HapII CCGG 3 cut(s) 140, 481, 601
HgaI GACGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 172
Hin1II CATG 4 cut(s) 74, 89, 147, 502
HinfI GANTC 3 cut(s) 317, 495, 586
HpaII CCGG 3 cut(s) 140, 481, 601
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 5 cut(s) 54, 274, 316, 322, 436
Hpy188III TCNNGA 3 cut(s) 71, 130, 293
Hpy8I GTNNAC 1 cut(s) 450
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 2 cut(s) 310, 524
HpyCH4V TGCA 3 cut(s) 502, 511, 532
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 200, 371, 380, 383, 508
HpyF3I CTNAG 5 cut(s) 25, 199, 332, 405, 538
Hsp92I GRCGYC 1 cut(s) 172
Hsp92II CATG 4 cut(s) 74, 89, 147, 502
Kzo9I GATC 2 cut(s) 100, 475
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 3 cut(s) 178, 361, 514
LweI GCATC 4 cut(s) 154, 212, 370, 478
MaeIII GTNAC 3 cut(s) 355, 554, 583
MalI GATC 2 cut(s) 102, 477
MboI GATC 2 cut(s) 100, 475
MboII GAAGA 2 cut(s) 33, 485
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 475
MluCI AATT 4 cut(s) 225, 251, 351, 591
MlyI GAGTC 1 cut(s) 580
MnlI CCTC 6 cut(s) 67, 78, 147, 205, 320, 547
MseI TTAA 1 cut(s) 233
MspI CCGG 3 cut(s) 140, 481, 601
MspR9I CCNGG 2 cut(s) 288, 602
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 6 cut(s) 122, 200, 371, 380, 383, 508
NciI CCSGG 1 cut(s) 602
NcoI CCATGG 1 cut(s) 85
NdeII GATC 2 cut(s) 100, 475
NlaIII CATG 4 cut(s) 74, 89, 147, 502
NlaIV GGNNCC 2 cut(s) 9, 83
NmuCI GTSAC 2 cut(s) 554, 583
NspI RCATGY 1 cut(s) 147
PagI TCATGA 1 cut(s) 70
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 317, 495
PflMI CCANNNNNTGG 3 cut(s) 287, 596, 622
PkrI GCNGC 4 cut(s) 193, 376, 385, 504
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PshAI GACNNNNGTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 2 cut(s) 9, 83
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 1 cut(s) 475
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 4 cut(s) 192, 375, 384, 503
Sau3AI GATC 2 cut(s) 100, 475
SchI GAGTC 1 cut(s) 580
ScrFI CCNGG 2 cut(s) 288, 602
SetI ASST 7 cut(s) 61, 70, 78, 139, 331, 507, 517
SfaNI GCATC 4 cut(s) 154, 212, 370, 478
SfcI CTRYAG 1 cut(s) 150
Sse9I AATT 4 cut(s) 225, 251, 351, 591
SsiI CCGC 2 cut(s) 377, 383
StyD4I CCNGG 2 cut(s) 286, 600
StyI CCWWGG 2 cut(s) 85, 346
TaaI ACNGT 2 cut(s) 310, 524
TasI AATT 4 cut(s) 225, 251, 351, 591
TatI WGTACW 1 cut(s) 447
TauI GCSGC 1 cut(s) 386
TfiI GAWTC 2 cut(s) 317, 495
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 3 cut(s) 340, 546, 623
TseFI GTSAC 2 cut(s) 554, 583
TseI GCWGC 3 cut(s) 191, 374, 502
Tsp45I GTSAC 2 cut(s) 554, 583
TspRI CASTG 3 cut(s) 340, 546, 623
Van91I CCANNNNNTGG 3 cut(s) 287, 596, 622
XceI RCATGY 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.