RchiOBHm_Chr6g0260261

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004425
Physical Location & Seq
Forward (+)
15333024 .. 15335691
2668 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23339

Sequence Viewer

Length: 678 bp
ATGGAGTGCACAATCTTTGAGGAGCATCCCTCGCAAGTCATGAGCTGTTACTTGATGATTTATGTGGATTCTCTAAAATTAGACTTTGTTGCTGCCATGAGCATGGTGGGCCACATTTTTGGTCTTGGGGATAGACACTTGGATAATATCCTCATGGATTTCTGTAGCGGTGATGTGGTACATATTGATTACAATGTTTGTTTTGATGAAAGGCAAAGACTAAAAATTCCAGAAATTGTACCCTTCTGCCTTACACAGACCATTGAAGCTGCATTAGGGATGACTGGAATAGAAGGTACCTTTAGGTCAAACTGTGAAGTGGTTATTGGTGTTTTGAGGAAGAACAAAGACATACTTTTGATGTTACTAGAAGTGTTTGTTTGGGACCCTCTGGTGGAATGGACACTGGGAGATTTTCATGATGCCGCAATTGGCGGAAATTCGTGTCCCGTTACAGGCACTGAACAAGAATGGAAGCTTGCGCAGAGCACAATGGAAACTGCTTCCAGTGGATTGTATTCTGCAACGAACGAACTTGGCAATGCATCTCTCAAAGCAAAGTCAGCTTCAGGTGATTTACAAAGCACGGTTCTTGCCATGAGGGATTGTGCACAAAGCCAGTGTTGCACTAATGGCATATGCTGGTGTTTCAAATCGTCATTCTACTTTGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006401 GO:0006402 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019439 GO:0019538 GO:0019637 GO:0030258 GO:0031323 GO:0031974 GO:0031981 GO:0032204 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034655 GO:0036211 GO:0042162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046486 GO:0046488 GO:0046700 GO:0046777 GO:0046834 GO:0046854 GO:0048519 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097159 GO:0140096 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901575 GO:2001020
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
FATC PI3_PI4_kinase SMG1 WD40

Protein Analysis

225

Amino Acids

24.98

Weight (kDa)

4.53

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 30 - 135 4.3e-32 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 483
Acc65I GGTACC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 296
AciI CCGC 3 cut(s) 168, 426, 435
AcsI RAATTY 2 cut(s) 225, 439
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 3 cut(s) 180, 240, 298
AfiI CCNNNNNNNGG 2 cut(s) 394, 455
AgsI TTSAA 2 cut(s) 266, 652
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 4 cut(s) 45, 269, 478, 566
AluI AGCT 4 cut(s) 45, 269, 478, 566
Alw21I GWGCWC 3 cut(s) 11, 491, 613
Alw44I GTGCAC 2 cut(s) 7, 609
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 1 cut(s) 109
ApaLI GTGCAC 2 cut(s) 7, 609
ApeKI GCWGC 2 cut(s) 92, 269
ApoI RAATTY 2 cut(s) 225, 439
Asp718I GGTACC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 2 cut(s) 109, 385
AsuHPI GGTGA 2 cut(s) 182, 584
AvaII GGWCC 1 cut(s) 385
BaeGI GKGCMC 2 cut(s) 11, 613
BanI GGYRCC 1 cut(s) 296
Bbv12I GWGCWC 3 cut(s) 11, 491, 613
BbvI GCAGC 2 cut(s) 79, 256
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 163
BisI GCNGC 3 cut(s) 93, 270, 426
BlsI GCNGC 3 cut(s) 94, 271, 427
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 2 cut(s) 109, 385
BmiI GGNNCC 3 cut(s) 298, 386, 387
BmrI ACTGGG 1 cut(s) 416
BmsI GCATC 3 cut(s) 34, 412, 554
BmuI ACTGGG 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 14, 46
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 455
Bse1I ACTGG 4 cut(s) 289, 411, 507, 619
Bse3DI GCAATG 1 cut(s) 547
BseGI GGATG 2 cut(s) 25, 285
BseLI CCNNNNNNNGG 2 cut(s) 394, 455
BseMI GCAATG 1 cut(s) 547
BseNI ACTGG 4 cut(s) 289, 411, 507, 619
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 11, 613
BseXI GCAGC 2 cut(s) 79, 256
BshFI GGCC 1 cut(s) 111
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 3 cut(s) 11, 491, 613
BslFI GGGAC 2 cut(s) 398, 432
BslI CCNNNNNNNGG 2 cut(s) 394, 455
BsmFI GGGAC 2 cut(s) 398, 432
BsnI GGCC 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 11, 491, 613
BspACI CCGC 3 cut(s) 168, 426, 435
BspANI GGCC 1 cut(s) 111
BspHI TCATGA 2 cut(s) 39, 418
BspLI GGNNCC 3 cut(s) 298, 386, 387
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 547
BsrI ACTGG 4 cut(s) 289, 411, 507, 619
Bst4CI ACNGT 2 cut(s) 314, 589
BstC8I GCNNGC 1 cut(s) 480
BstF5I GGATG 2 cut(s) 25, 285
BstHHI GCGC 1 cut(s) 484
BstMWI GCNNNNNNNGC 5 cut(s) 31, 108, 563, 624, 633
BstSFI CTRYAG 1 cut(s) 163
BstSLI GKGCMC 2 cut(s) 11, 613
BstV1I GCAGC 2 cut(s) 79, 256
BstXI CCANNNNNNTGG 2 cut(s) 103, 119
BsuRI GGCC 1 cut(s) 111
BtsCI GGATG 2 cut(s) 25, 285
BtsIMutI CAGTG 4 cut(s) 404, 459, 514, 626
Cac8I GCNNGC 1 cut(s) 480
CaiI CAGNNNCTG 1 cut(s) 461
CciI TCATGA 2 cut(s) 39, 418
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 2 cut(s) 109, 385
Csp6I GTAC 3 cut(s) 179, 239, 297
CviAII CATG 6 cut(s) 40, 97, 103, 154, 419, 598
CviJI RGCY 6 cut(s) 45, 111, 269, 478, 566, 618
CviKI_1 RGCY 6 cut(s) 45, 111, 269, 478, 566, 618
CviQI GTAC 3 cut(s) 179, 239, 297
EciI GGCGGA 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 385
EcoT22I ATGCAT 1 cut(s) 547
FaeI CATG 6 cut(s) 43, 100, 106, 157, 422, 601
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 2 cut(s) 398, 432
FatI CATG 6 cut(s) 39, 96, 102, 153, 418, 597
FauNDI CATATG 1 cut(s) 638
Fnu4HI GCNGC 3 cut(s) 93, 270, 426
FokI GGATG 2 cut(s) 12, 292
Fsp4HI GCNGC 3 cut(s) 93, 270, 426
FspBI CTAG 1 cut(s) 368
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 1 cut(s) 483
GluI GCNGC 3 cut(s) 93, 270, 426
HaeIII GGCC 1 cut(s) 111
HhaI GCGC 1 cut(s) 484
Hin1II CATG 6 cut(s) 43, 100, 106, 157, 422, 601
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 1 cut(s) 68
HphI GGTGA 2 cut(s) 182, 584
Hpy166II GTNNAC 2 cut(s) 9, 611
Hpy188I TCNGA 1 cut(s) 677
Hpy188III TCNNGA 3 cut(s) 40, 230, 419
Hpy8I GTNNAC 2 cut(s) 9, 611
HpyAV CCTTC 2 cut(s) 253, 287
HpyCH4III ACNGT 2 cut(s) 314, 589
HpyCH4V TGCA 6 cut(s) 9, 272, 524, 545, 611, 627
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 108, 563, 624, 633
Hsp92II CATG 6 cut(s) 43, 100, 106, 157, 422, 601
HspAI GCGC 1 cut(s) 482
KflI GGGWCCC 1 cut(s) 385
KpnI GGTACC 1 cut(s) 300
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 9 cut(s) 243, 270, 377, 392, 441, 520, 555, 628, 632
Lsp1109I GCAGC 2 cut(s) 79, 256
LweI GCATC 3 cut(s) 34, 412, 554
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 3 cut(s) 47, 363, 451
MboII GAAGA 1 cut(s) 352
MfeI CAATTG 1 cut(s) 429
MhlI GDGCHC 3 cut(s) 11, 491, 613
MluCI AATT 5 cut(s) 77, 225, 234, 429, 439
MnlI CCTC 6 cut(s) 13, 40, 161, 330, 399, 594
Mph1103I ATGCAT 1 cut(s) 547
MslI CAYNNNNRTG 1 cut(s) 101
MunI CAATTG 1 cut(s) 429
MwoI GCNNNNNNNGC 5 cut(s) 31, 108, 563, 624, 633
NdeI CATATG 1 cut(s) 638
NlaIII CATG 6 cut(s) 43, 100, 106, 157, 422, 601
NlaIV GGNNCC 3 cut(s) 298, 386, 387
NsbI TGCGCA 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 547
PagI TCATGA 2 cut(s) 39, 418
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 3 cut(s) 94, 271, 427
PpuMI RGGWCCY 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 298, 386, 387
PspPI GGNCC 2 cut(s) 109, 385
PspPPI RGGWCCY 1 cut(s) 385
PstNI CAGNNNCTG 1 cut(s) 461
RsaI GTAC 3 cut(s) 180, 240, 298
RsaNI GTAC 3 cut(s) 179, 239, 297
RseI CAYNNNNRTG 1 cut(s) 101
SatI GCNGC 3 cut(s) 93, 270, 426
Sau96I GGNCC 2 cut(s) 109, 385
SduI GDGCHC 3 cut(s) 11, 491, 613
SetI ASST 8 cut(s) 47, 271, 298, 302, 308, 480, 568, 574
SfaNI GCATC 3 cut(s) 34, 412, 554
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 101
Sse9I AATT 5 cut(s) 77, 225, 234, 429, 439
SsiI CCGC 3 cut(s) 168, 426, 435
SspMI CTAG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 314, 589
TasI AATT 5 cut(s) 77, 225, 234, 429, 439
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 1 cut(s) 68
TscAI CASTG 4 cut(s) 411, 466, 514, 626
TseI GCWGC 2 cut(s) 92, 269
TspDTI ATGAA 2 cut(s) 222, 407
TspRI CASTG 4 cut(s) 411, 466, 514, 626
VneI GTGCAC 2 cut(s) 7, 609
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 2 cut(s) 225, 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XspI CTAG 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.