AT2G35920

RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007834
Physical Location & Seq
Forward (+)
15075343 .. 15080812
5470 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35920.2

Sequence Viewer

Length: 2988 bp
ATGCCTCCTCACGGTCCTAATTCTCAAGGTGGTCGTCGAGGCGGAGGTCATTCCTCCGGTCGTCGTGGTGGACGCGGCGGCGGCGGTCGTGGCGGTGGTGGTGGCGGCCGGGGGGAACAGCGATGGTGGGATCCTGTGTGGAGAGCTGAGCGTTTGCGCCAGCAACAAGCTGAGATGGAAGTACTTGATGAGAATGAATGGTGGAACAAGATTGAGCAATGGAAAACAGGAGGAGAACAAGAGATGCTAATTAAGCGTAATTTTAGCCGTGGAGATCAGCAAACACTTTCTGATATGGCTTTGCAGATGGGTCTTTACTTTCATGCGTACAATAAAGGGAAGGCTCTTGTTGTTAGCAAAGTTCCTTTACCAGATTATCGAGCGGATCTTGATGAACGGCATGGATCCACTCAGAAAGAGATTAAAATGTCCACAGAGACTGAGAGAAAACTTGGAAGTCTTTTGAAAACAACACAAGAATCAGGGTCCTCTGGTGCCTCTGCTTCAGCTTTTAACGATCAACAGGATCGTACTTCAACTCTTGGCTTGAAAAGACCTGATTCTGCTTCCAAATTGCCGGATTCTCTTGAGAAAGAAAAATTCAGTTTTGCACTCAAGGAAAGGCAGGAAAAGCTTAAGGCAACTGAGAGTGTAAAAGCACTTAAAGCGTTCAGAGAAAAGCTACCTGCTTTTAAAATGAAAGAGGAGTTTCTTAACTCTGTTTCACAAAACCAGGTATTGGTAGTTTCAGGAGAGACCGGGTGTGGTAAAACAACACAGCTTCCTCAATTTATATTGGAAGAAGAAATATCATCCCTAAGAGGTGCTGATTGCAACATAATTTGCACTCAACCTCGACGTATATCGGCCATATCTGTTGCTTCTCGTATATCTGCTGAAAGAGGTGAATCCATTGGTGAATCTGTGGGTTATCAGATTCGTCTAGAATCAAAGCGTTCTGATCAAACACGATTGCTATTTTGCACTACTGGTGTTTTACTCCGGCGGCTGATTGAGGATCCTAATCTAACTAACGTAAGCCATTTGCTCGTGGATGAGATTCACGAAAGAGGCATGAATGAAGATTTTCTACTAATCATACTACGCGATCTCCTTCCTCGACGCCCAGATTTGCGTCTTATTCTGATGAGTGCTACGATTAACGCTGACATGTTCTCAACATACTTTGGGAACTCTCCCACTATGCACATCCCGGGTTTCACATTTCCTGTGGCAGAGTTATTCCTAGAAGATGTATTGGAGAAAAGTCGTTATAATATTAAGTCGTCTGACTCGGGGAATTATCAAGGTAGTTCGAGAGGTAGAAGAAGAGAATCAGAATCCAAGAAAGATGATCTGACTACTCTTTTTGAGGATATTGACATCAATTCTCACTATAAAAGTTATAGCTCGGCCACAAGAAATTCTCTTGAAGCATGGTCTGGTGCACAGATTGATGTGGATCTGGTGGAGGCAACAATTGAACATATTTGTCGTCTTGAAGGTGGTGGGGCGATTCTTGTCTTCCTTACTGGCTGGGATGAGATTTCAAAACTGCTTGAAAAAATTAACATGAACAACTTTCTTGGAGATTCGAGCAAGTTCTTAGTTCTTCCTTTACATGGTTCAATGCCGACGGTTAATCAACGTGAAATTTTTGACCGTCCACCTCCTAACAAACGGAAAATAGTTTTGGCTACAAACATTGCCGAGAGTAGTATTACAATTGATGATGTTGTATATGTTGTGGATTGTGGTAAAGCAAAGGAAACTAGCTATGATGCTTTGAACAAGGTGGCATGTCTCTTGCCATCATGGATATCAAAGGCTTCAGCTCACCAGAGACGAGGTCGTGCTGGACGTGTTCAAGCTGGAGTTTGTTATAGGTTGTATCCAAAAGTTATCTATGATGCTTTTCCACAGTATCAGTTACCAGAAATCATACGGACTCCATTACAAGAGCTATGCCTGCATATCAAAAGTTTGCAAGTTGGATCTATAGGCTCATTCTTGGCAAAGGCACTGCAGCCACCAGATGCACTTGCTGTTGAGAATGCTATTGAACTTCTCAAAACAATTGGGGCTTTAAATGACGTGGAAGAGCTTACACCCCTTGGTCGGCATCTTTGCACTCTACCCGTGGACCCAAATATTGGAAAGATGCTTCTGATTGGAGCTATCTTCCAGTGCGTCAATCCTGCTTTGACAATCGCTGCTGCTCTGGCGTATCGTAGCCCATTTGTCTTACCATTAAATAGGAAAGAGGAAGCTGATGAAGCCAAACGATATTTTGCTGGTGATTCCTGCAGTGATCACATTGCTCTTCTTAAGGCATATGAGGGATATCGGGATGCAAAGCGTGGTGGAAACGAAAAAGATTTTTGTTGGCAAAACTTTCTTTCCCCTGTAACTCTAAGGATGATGGAGGATATGAGAAACCAGTTTCTTGATCTTCTATCAGATATAGGGTTTGTAGACAAATCAAAGCCAAATGCATACAACCAATATAGCTACGATATGGAGATGATAAGTGCGGTTCTATGTGCGGGGCTGTACCCAAATGTTGTCCAATGCAAAAGAAGAGGGAAGCGCACTGCGTTCTATACTAAAGAGCTGGGTAAAGTTGATATCCACCCTGGATCTGTCAACGCAAGAGTGAATCTCTTCTCTTTGCCGTACTTGGTCTACAGCGAAAAGGTGAAGACGACAAGTGTTTATATCCGAGACTCGACAAATATATCAGACTATGCACTCCTTATGTTTGGTGGCAATCTTATCCCAAGCAAAACCGGGGAAGGTATAGAGATGTTGGGAGGGTATCTCCATTTCTCAGCCTCGAAGAACATCTTAGAACTGATACAGAGATTGAGGGGAGAGGTGGACAAGCTACTGAATAAGAAGATAGAGGATCCGAGTTTGGACATAACAGTGGAGGGGAAAGGAGTGGTCTCAGCTGTGGTGGAGTTGTTACGTAGCCAAAAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

995

Amino Acids

111.04

Weight (kDa)

8.28

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 177 - 331 4.2e-10 DEAD/DEAH box helicase
Helicase_C PF00271 436 - 564 7.3e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 628 - 656 4.7e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 657 - 717 2.6e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 785 - 866 6.8e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 876 - 933 2.1e-20 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1275
Acc36I ACCTGC 1 cut(s) 694
AccB1I GGYRCC 1 cut(s) 494
AccB7I CCANNNNNTGG 2 cut(s) 739, 2153
AccBSI CCGCTC 1 cut(s) 383
AccI GTMKAC 2 cut(s) 2475, 2685
AccII CGCG 2 cut(s) 75, 1107
AcoI YGGCCR 3 cut(s) 106, 867, 1413
AcsI RAATTY 3 cut(s) 599, 1423, 1653
AcuI CTGAAG 2 cut(s) 489, 1815
AcyI GRCGYC 1 cut(s) 1123
AfaI GTAC 5 cut(s) 183, 329, 532, 2555, 2678
AfiI CCNNNNNNNGG 5 cut(s) 11, 739, 1622, 2118, 2153
AflII CTTAAG 2 cut(s) 635, 2327
AflIII ACRYGT 2 cut(s) 1170, 1861
AjiI CACGTC 2 cut(s) 1862, 2095
AjnI CCWGG 2 cut(s) 732, 2635
AjuI GAANNNNNNNTTGG 2 cut(s) 1676, 1708
Alw21I GWGCWC 1 cut(s) 1450
Alw26I GTCTC 6 cut(s) 431, 749, 1808, 1837, 2718, 2953
Alw44I GTGCAC 1 cut(s) 1446
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 2 cut(s) 1213, 1294
AoxI GGCC 3 cut(s) 106, 867, 1413
ApaLI GTGCAC 1 cut(s) 1446
ApeKI GCWGC 3 cut(s) 2026, 2213, 2216
ApoI RAATTY 3 cut(s) 599, 1423, 1653
Asp700I GAANNNNTTC 2 cut(s) 1086, 2663
AspLEI GCGC 2 cut(s) 159, 2592
AspS9I GGNCC 3 cut(s) 14, 486, 2143
AsuC2I CCSGG 5 cut(s) 110, 760, 1214, 1215, 2791
AsuHPI GGTGA 5 cut(s) 917, 929, 1829, 2309, 2710
AvaI CYCGRG 2 cut(s) 1213, 1294
AvaII GGWCC 3 cut(s) 14, 486, 2143
BaeGI GKGCMC 1 cut(s) 1450
BamHI GGATCC 4 cut(s) 130, 404, 1018, 2908
BanI GGYRCC 1 cut(s) 494
BarI GAAGNNNNNNTAC 2 cut(s) 1074, 1106
BauI CACGAG 1 cut(s) 1049
BbsI GAAGAC 2 cut(s) 1516, 2708
Bbv12I GWGCWC 1 cut(s) 1450
BbvI GCAGC 3 cut(s) 2038, 2200, 2203
BccI CCATC 5 cut(s) 117, 169, 301, 1819, 2416
BceAI ACGGC 3 cut(s) 252, 413, 2659
BciT130I CCWGG 2 cut(s) 734, 2637
BciVI GTATCC 1 cut(s) 1902
BclI TGATCA 2 cut(s) 961, 2311
BcnI CCSGG 5 cut(s) 110, 760, 1214, 1215, 2791
BcoDI GTCTC 6 cut(s) 431, 749, 1808, 1837, 2718, 2953
BfaI CTAG 3 cut(s) 944, 1247, 1773
BfmI CTRYAG 4 cut(s) 1998, 2024, 2305, 2686
BfrI CTTAAG 2 cut(s) 635, 2327
BfuAI ACCTGC 1 cut(s) 694
BfuI GTATCC 1 cut(s) 1902
BisI GCNGC 8 cut(s) 76, 79, 82, 106, 1007, 2027, 2214, 2217
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 8 cut(s) 77, 80, 83, 107, 1008, 2028, 2215, 2218
BmcAI AGTACT 1 cut(s) 183
Bme1390I CCNGG 7 cut(s) 110, 734, 760, 1214, 1215, 2637, 2791
Bme18I GGWCC 3 cut(s) 14, 486, 2143
BmeT110I CYCGRG 2 cut(s) 1213, 1294
BmgBI CACGTC 2 cut(s) 1862, 2095
BmgT120I GGNCC 3 cut(s) 14, 486, 2143
BmiI GGNNCC 7 cut(s) 132, 406, 487, 496, 1020, 2145, 2910
BmrFI CCNGG 7 cut(s) 110, 734, 760, 1214, 1215, 2637, 2791
BmsI GCATC 7 cut(s) 234, 1771, 1900, 2026, 2131, 2151, 2341
BpiI GAAGAC 2 cut(s) 1516, 2708
BplI GAGNNNNNCTC 4 cut(s) 2646, 2678, 2805, 2837
BpmI CTGGAG 1 cut(s) 1893
Bpu1102I GCTNAGC 1 cut(s) 147
BpuEI CTTGAG 3 cut(s) 9, 599, 608
BpuMI CCSGG 5 cut(s) 110, 760, 1214, 1215, 2791
BsaAI YACGTR 1 cut(s) 2972
BsaBI GATNNNNATC 2 cut(s) 1023, 1461
BsaHI GRCGYC 1 cut(s) 1123
BsaI GGTCTC 2 cut(s) 749, 2953
BsaJI CCNNGG 7 cut(s) 109, 268, 1213, 2113, 2139, 2635, 2790
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 1095, 1125
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 739, 1622, 2118, 2153
Bse1I ACTGG 4 cut(s) 994, 1537, 2185, 2440
Bse3DI GCAATG 3 cut(s) 224, 1704, 2316
Bse8I GATNNNNATC 2 cut(s) 1023, 1461
BseBI CCWGG 2 cut(s) 734, 2637
BseDI CCNNGG 7 cut(s) 109, 268, 1213, 2113, 2139, 2635, 2790
BseGI GGATG 6 cut(s) 812, 1060, 1209, 1546, 2356, 2424
BseJI GATNNNNATC 2 cut(s) 1023, 1461
BseLI CCNNNNNNNGG 5 cut(s) 11, 739, 1622, 2118, 2153
BseMI GCAATG 3 cut(s) 224, 1704, 2316
BseMII CTCAG 7 cut(s) 138, 162, 425, 432, 636, 2844, 2964
BseNI ACTGG 4 cut(s) 994, 1537, 2185, 2440
BseRI GAGGAG 2 cut(s) 246, 719
BseSI GKGCMC 1 cut(s) 1450
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 3 cut(s) 2038, 2200, 2203
BseYI CCCAGC 2 cut(s) 1536, 2614
Bsh1236I CGCG 2 cut(s) 75, 1107
Bsh1285I CGRYCG 3 cut(s) 61, 88, 109
BshFI GGCC 3 cut(s) 108, 869, 1415
BshNI GGYRCC 1 cut(s) 494
BsiEI CGRYCG 3 cut(s) 61, 88, 109
BsiHKAI GWGCWC 1 cut(s) 1450
BsiHKCI CYCGRG 2 cut(s) 1213, 1294
BsiSI CCGG 7 cut(s) 57, 109, 578, 759, 1003, 1214, 2790
BslI CCNNNNNNNGG 5 cut(s) 11, 739, 1622, 2118, 2153
BsmAI GTCTC 6 cut(s) 431, 749, 1808, 1837, 2718, 2953
BsmBI CGTCTC 1 cut(s) 1837
BsmI GAATGC 1 cut(s) 2059
BsnI GGCC 3 cut(s) 108, 869, 1415
Bso31I GGTCTC 2 cut(s) 749, 2953
BsoBI CYCGRG 2 cut(s) 1213, 1294
Bsp1286I GDGCHC 1 cut(s) 1450
Bsp1720I GCTNAGC 1 cut(s) 147
BspANI GGCC 3 cut(s) 108, 869, 1415
BspCNI CTCAG 7 cut(s) 139, 163, 424, 433, 637, 2843, 2963
BspFNI CGCG 2 cut(s) 75, 1107
BspLI GGNNCC 7 cut(s) 132, 406, 487, 496, 1020, 2145, 2910
BspMAI CTGCAG 2 cut(s) 2028, 2309
BspMI ACCTGC 1 cut(s) 694
BspQI GCTCTTC 2 cut(s) 2094, 2328
BspT107I GGYRCC 1 cut(s) 494
BspTI CTTAAG 2 cut(s) 635, 2327
BspTNI GGTCTC 2 cut(s) 749, 2953
BsrBI CCGCTC 1 cut(s) 383
BsrDI GCAATG 3 cut(s) 224, 1704, 2316
BsrI ACTGG 4 cut(s) 994, 1537, 2185, 2440
BssECI CCNNGG 7 cut(s) 109, 268, 1213, 2113, 2139, 2635, 2790
BssNI GRCGYC 1 cut(s) 1123
BssSI CACGAG 1 cut(s) 1049
BssT1I CCWWGG 1 cut(s) 2113
Bst2BI CACGAG 1 cut(s) 1049
Bst2UI CCWGG 2 cut(s) 734, 2637
Bst4CI ACNGT 5 cut(s) 14, 1639, 1664, 1923, 2929
Bst6I CTCTTC 5 cut(s) 1324, 2094, 2328, 2575, 2669
BstACI GRCGYC 1 cut(s) 1123
BstAFI CTTAAG 2 cut(s) 635, 2327
BstBAI YACGTR 1 cut(s) 2972
BstC8I GCNNGC 2 cut(s) 161, 1970
BstDSI CCRYGG 2 cut(s) 268, 2139
BstF5I GGATG 6 cut(s) 812, 1060, 1209, 1546, 2356, 2424
BstFNI CGCG 2 cut(s) 75, 1107
BstHHI GCGC 2 cut(s) 159, 2592
BstMAI GTCTC 6 cut(s) 431, 749, 1808, 1837, 2718, 2953
BstMCI CGRYCG 3 cut(s) 61, 88, 109
BstMWI GCNNNNNNNGC 8 cut(s) 81, 90, 253, 631, 665, 1969, 2222, 2276
BstNI CCWGG 2 cut(s) 734, 2637
BstNSI RCATGY 2 cut(s) 1174, 1803
BstSCI CCNGG 7 cut(s) 108, 732, 758, 1212, 1213, 2635, 2789
BstSFI CTRYAG 4 cut(s) 1998, 2024, 2305, 2686
BstSLI GKGCMC 1 cut(s) 1450
BstSNI TACGTA 1 cut(s) 2972
BstUI CGCG 2 cut(s) 75, 1107
BstV1I GCAGC 3 cut(s) 2038, 2200, 2203
BstV2I GAAGAC 2 cut(s) 1516, 2708
BstX2I RGATCY 8 cut(s) 130, 385, 404, 1018, 1462, 1994, 2639, 2908
BstYI RGATCY 8 cut(s) 130, 385, 404, 1018, 1462, 1994, 2639, 2908
BstZI CGGCCG 1 cut(s) 106
BsuI GTATCC 1 cut(s) 1902
BsuRI GGCC 3 cut(s) 108, 869, 1415
BtgI CCRYGG 2 cut(s) 268, 2139
BtgZI GCGATG 1 cut(s) 136
BtrI CACGTC 2 cut(s) 1862, 2095
BtsCI GGATG 6 cut(s) 812, 1060, 1209, 1546, 2356, 2424
BtsI GCAGTG 3 cut(s) 2021, 2314, 2592
BtsIMutI CAGTG 5 cut(s) 2021, 2192, 2314, 2592, 2934
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 2 cut(s) 161, 1970
CaiI CAGNNNCTG 1 cut(s) 440
CfoI GCGC 2 cut(s) 159, 2592
Cfr13I GGNCC 3 cut(s) 14, 486, 2143
Cfr9I CCCGGG 1 cut(s) 1213
CseI GACGC 4 cut(s) 81, 1124, 1131, 2179
CsiI ACCWGGT 1 cut(s) 732
Csp6I GTAC 5 cut(s) 182, 328, 531, 2554, 2677
CviAII CATG 9 cut(s) 323, 401, 1075, 1171, 1437, 1573, 1622, 1800, 1815
CviQI GTAC 5 cut(s) 182, 328, 531, 2554, 2677
DraI TTTAAA 2 cut(s) 694, 2088
EaeI YGGCCR 3 cut(s) 106, 867, 1413
EagI CGGCCG 1 cut(s) 106
Eam1104I CTCTTC 5 cut(s) 1324, 2094, 2328, 2575, 2669
EarI CTCTTC 5 cut(s) 1324, 2094, 2328, 2575, 2669
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 2972
Eco130I CCWWGG 1 cut(s) 2113
Eco31I GGTCTC 2 cut(s) 749, 2953
Eco32I GATATC 3 cut(s) 1821, 2345, 2629
Eco47I GGWCC 3 cut(s) 14, 486, 2143
Eco52I CGGCCG 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 489, 1815
Eco88I CYCGRG 2 cut(s) 1213, 1294
EcoO109I RGGNCCY 1 cut(s) 486
EcoRII CCWGG 2 cut(s) 732, 2635
EcoRV GATATC 3 cut(s) 1821, 2345, 2629
EcoT14I CCWWGG 1 cut(s) 2113
EcoT22I ATGCAT 1 cut(s) 2497
ErhI CCWWGG 1 cut(s) 2113
Esp3I CGTCTC 1 cut(s) 1837
FaeI CATG 9 cut(s) 326, 404, 1078, 1174, 1440, 1576, 1625, 1803, 1818
FalI AAGNNNNNCTT 2 cut(s) 2831, 2863
FatI CATG 9 cut(s) 322, 400, 1074, 1170, 1436, 1572, 1621, 1799, 1814
FauI CCCGC 1 cut(s) 2539
FauNDI CATATG 1 cut(s) 2335
FbaI TGATCA 2 cut(s) 961, 2311
FblI GTMKAC 2 cut(s) 2475, 2685
Fnu4HI GCNGC 8 cut(s) 76, 79, 82, 106, 1007, 2027, 2214, 2217
FokI GGATG 6 cut(s) 799, 1067, 1196, 1553, 2363, 2431
Fsp4HI GCNGC 8 cut(s) 76, 79, 82, 106, 1007, 2027, 2214, 2217
FspBI CTAG 3 cut(s) 944, 1247, 1773
GlaI GCGC 2 cut(s) 158, 2591
GluI GCNGC 8 cut(s) 76, 79, 82, 106, 1007, 2027, 2214, 2217
GsaI CCCAGC 2 cut(s) 1540, 2618
GsuI CTGGAG 1 cut(s) 1893
HaeIII GGCC 3 cut(s) 108, 869, 1415
HapII CCGG 7 cut(s) 57, 109, 578, 759, 1003, 1214, 2790
HgaI GACGC 4 cut(s) 81, 1124, 1131, 2179
HhaI GCGC 2 cut(s) 159, 2592
Hin1I GRCGYC 1 cut(s) 1123
Hin1II CATG 9 cut(s) 326, 404, 1078, 1174, 1440, 1576, 1625, 1803, 1818
Hin6I GCGC 2 cut(s) 157, 2590
HinP1I GCGC 2 cut(s) 157, 2590
HincII GTYRAC 1 cut(s) 2647
HindII GTYRAC 1 cut(s) 2647
HindIII AAGCTT 1 cut(s) 632
HpaII CCGG 7 cut(s) 57, 109, 578, 759, 1003, 1214, 2790
HphI GGTGA 5 cut(s) 917, 929, 1829, 2309, 2710
Hpy166II GTNNAC 9 cut(s) 71, 432, 1448, 1667, 2143, 2476, 2647, 2686, 2881
Hpy8I GTNNAC 9 cut(s) 71, 432, 1448, 1667, 2143, 2476, 2647, 2686, 2881
Hpy99I CGWCG 5 cut(s) 39, 66, 861, 1125, 1639
HpyAV CCTTC 4 cut(s) 334, 1124, 1496, 2789
HpyCH4III ACNGT 5 cut(s) 14, 1639, 1664, 1923, 2929
HpyCH4IV ACGT 6 cut(s) 859, 1035, 1648, 1861, 2094, 2971
HpyF10VI GCNNNNNNNGC 8 cut(s) 81, 90, 253, 631, 665, 1969, 2222, 2276
HpySE526I ACGT 6 cut(s) 859, 1035, 1648, 1861, 2094, 2971
Hsp92I GRCGYC 1 cut(s) 1123
Hsp92II CATG 9 cut(s) 326, 404, 1078, 1174, 1440, 1576, 1625, 1803, 1818
HspAI GCGC 2 cut(s) 157, 2590
Ksp22I TGATCA 2 cut(s) 961, 2311
LguI GCTCTTC 2 cut(s) 2094, 2328
LmnI GCTCC 1 cut(s) 2174
Lsp1109I GCAGC 3 cut(s) 2038, 2200, 2203
LweI GCATC 7 cut(s) 234, 1771, 1900, 2026, 2131, 2151, 2341
MabI ACCWGGT 1 cut(s) 732
MaeI CTAG 3 cut(s) 944, 1247, 1773
MaeII ACGT 6 cut(s) 859, 1035, 1648, 1861, 2094, 2971
MaeIII GTNAC 3 cut(s) 1929, 2407, 2967
MbiI CCGCTC 1 cut(s) 383
MfeI CAATTG 3 cut(s) 1479, 1725, 2076
MflI RGATCY 8 cut(s) 130, 385, 404, 1018, 1462, 1994, 2639, 2908
MhlI GDGCHC 1 cut(s) 1450
MlyI GAGTC 3 cut(s) 1286, 1942, 2720
MmeI TCCRAC 1 cut(s) 1972
Mph1103I ATGCAT 1 cut(s) 2497
MroXI GAANNNNTTC 2 cut(s) 1086, 2663
MslI CAYNNNNRTG 1 cut(s) 2927
MspA1I CMGCKG 1 cut(s) 2954
MspCI CTTAAG 2 cut(s) 635, 2327
MspI CCGG 7 cut(s) 57, 109, 578, 759, 1003, 1214, 2790
MspR9I CCNGG 7 cut(s) 110, 734, 760, 1214, 1215, 2637, 2791
MunI CAATTG 3 cut(s) 1479, 1725, 2076
Mva1269I GAATGC 1 cut(s) 2059
MvaI CCWGG 2 cut(s) 734, 2637
MvnI CGCG 2 cut(s) 75, 1107
MwoI GCNNNNNNNGC 8 cut(s) 81, 90, 253, 631, 665, 1969, 2222, 2276
NciI CCSGG 5 cut(s) 110, 760, 1214, 1215, 2791
NdeI CATATG 1 cut(s) 2335
NlaIII CATG 9 cut(s) 326, 404, 1078, 1174, 1440, 1576, 1625, 1803, 1818
NlaIV GGNNCC 7 cut(s) 132, 406, 487, 496, 1020, 2145, 2910
NmeAIII GCCGAG 2 cut(s) 1391, 1735
NsiI ATGCAT 1 cut(s) 2497
NspI RCATGY 2 cut(s) 1174, 1803
PciI ACATGT 1 cut(s) 1170
PciSI GCTCTTC 2 cut(s) 2094, 2328
PcsI WCGNNNNNNNCGW 1 cut(s) 1858
PctI GAATGC 1 cut(s) 2059
PdmI GAANNNNTTC 2 cut(s) 1086, 2663
PflFI GACNNNGTC 1 cut(s) 1848
PflMI CCANNNNNTGG 2 cut(s) 739, 2153
PkrI GCNGC 8 cut(s) 77, 80, 83, 107, 1008, 2028, 2215, 2218
PleI GAGTC 3 cut(s) 1286, 1942, 2720
PpsI GAGTC 3 cut(s) 1286, 1942, 2720
Ppu21I YACGTR 1 cut(s) 2972
PpuMI RGGWCCY 1 cut(s) 486
PscI ACATGT 1 cut(s) 1170
PsiI TTATAA 1 cut(s) 1275
Psp5II RGGWCCY 1 cut(s) 486
Psp6I CCWGG 2 cut(s) 732, 2635
PspFI CCCAGC 2 cut(s) 1536, 2614
PspGI CCWGG 2 cut(s) 732, 2635
PspN4I GGNNCC 7 cut(s) 132, 406, 487, 496, 1020, 2145, 2910
PspPI GGNCC 3 cut(s) 14, 486, 2143
PspPPI RGGWCCY 1 cut(s) 486
PstI CTGCAG 2 cut(s) 2028, 2309
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 8 cut(s) 130, 385, 404, 1018, 1462, 1994, 2639, 2908
PsyI GACNNNGTC 1 cut(s) 1848
PvuII CAGCTG 1 cut(s) 2954
RsaI GTAC 5 cut(s) 183, 329, 532, 2555, 2678
RsaNI GTAC 5 cut(s) 182, 328, 531, 2554, 2677
RseI CAYNNNNRTG 1 cut(s) 2927
SapI GCTCTTC 2 cut(s) 2094, 2328
SatI GCNGC 8 cut(s) 76, 79, 82, 106, 1007, 2027, 2214, 2217
Sau96I GGNCC 3 cut(s) 14, 486, 2143
ScaI AGTACT 1 cut(s) 183
SchI GAGTC 3 cut(s) 1286, 1942, 2720
ScrFI CCNGG 7 cut(s) 110, 734, 760, 1214, 1215, 2637, 2791
SduI GDGCHC 1 cut(s) 1450
SexAI ACCWGGT 1 cut(s) 732
SfaNI GCATC 7 cut(s) 234, 1771, 1900, 2026, 2131, 2151, 2341
SfcI CTRYAG 4 cut(s) 1998, 2024, 2305, 2686
SinI GGWCC 3 cut(s) 14, 486, 2143
SmaI CCCGGG 1 cut(s) 1215
SmiMI CAYNNNNRTG 1 cut(s) 2927
SmlI CTYRAG 5 cut(s) 24, 587, 614, 635, 2327
SmoI CTYRAG 5 cut(s) 24, 587, 614, 635, 2327
SnaBI TACGTA 1 cut(s) 2972
SspI AATATT 2 cut(s) 1279, 2152
SspMI CTAG 3 cut(s) 944, 1247, 1773
StyD4I CCNGG 7 cut(s) 108, 732, 758, 1212, 1213, 2635, 2789
StyI CCWWGG 1 cut(s) 2113
TaaI ACNGT 5 cut(s) 14, 1639, 1664, 1923, 2929
TaiI ACGT 6 cut(s) 862, 1038, 1651, 1864, 2097, 2974
TaqI TCGA 8 cut(s) 37, 379, 856, 1120, 1316, 1595, 2729, 2837
TatI WGTACW 1 cut(s) 181
TauI GCSGC 5 cut(s) 78, 81, 84, 108, 1009
TscAI CASTG 5 cut(s) 2028, 2192, 2314, 2599, 2934
TseI GCWGC 3 cut(s) 2026, 2213, 2216
TspDTI ATGAA 8 cut(s) 210, 311, 408, 713, 1091, 1095, 1589, 2289
TspGWI ACGGA 2 cut(s) 1696, 1960
TspMI CCCGGG 1 cut(s) 1213
TspRI CASTG 5 cut(s) 2028, 2192, 2314, 2599, 2934
Tth111I GACNNNGTC 1 cut(s) 1848
Van91I CCANNNNNTGG 2 cut(s) 739, 2153
Vha464I CTTAAG 2 cut(s) 635, 2327
VneI GTGCAC 1 cut(s) 1446
VpaK11BI GGWCC 3 cut(s) 14, 486, 2143
XapI RAATTY 3 cut(s) 599, 1423, 1653
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 2 cut(s) 1174, 1803
XmaI CCCGGG 1 cut(s) 1213
XmiI GTMKAC 2 cut(s) 2475, 2685
XmnI GAANNNNTTC 2 cut(s) 1086, 2663
XspI CTAG 3 cut(s) 944, 1247, 1773
ZrmI AGTACT 1 cut(s) 183
Zsp2I ATGCAT 1 cut(s) 2497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.