FvH4_6g20440

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007834
Physical Location & Seq
Reverse (-)
14076227 .. 14085591
9365 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20440.t2

Sequence Viewer

Length: 3114 bp
ATGTGTTGTTTGCTCTTAACTCTGCGCACAACCACCGCCCGCTTACTACTTCCCACTCACTTCTTCGCCGCTCCACGACTCCTCACATCCCGCTCCGCCTTCTCAGCTCTCGCCATGTCCAACCGCCCCAACTTCCACGGCGGCCGCAGGGGTGGGGGCCCTAACTCCCGCGGCGGCGGCCGTCGTGGAGGAGGCGGTGGCGGTCGCGGAGGTGGTGAGCAGCGCTGGTGGGACCCGGTCTGGCGAGCTGAGCGACTGAGGCAACAAGCCGCAGAGATGGAAGTACTTGATTCGAGTGAGTATTGGGGGAAGATGGAGCAGTTTAAAAGCGGAGCGGAGCAGGAGATGATAATCAGGCGCAATTTTAGTCGGCATGATCAGCAAACGCTATATGATATGGCCTATGAGCTAGGGCTTCACTTCCATGCGTATAATAAGGGGAAGGCTCTTGTGGTCAGTAAGGTTCCGTTGCCGGATTACCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGGATGTCTACAGAGACTGCAGAGAGAGTTGGGAGTCTTTTGCAGAGCTCGCAGGGGCAGGGAAGCAAGCAAGCTTTTGGTAATGTGGCTTTAGTGGCGTCTGGGTCTGGCCAGGGAAACAAACAAACACTTGTTGTTGAAAATACAAACAAACCCAGCTCCAGGTTGGAGGCTGAGACTGTTAAGGAGAAAGAGAAACTTAGCCTTCAACTCAAAGAGTTGCAAGAAAAGATGAAGGTGAGTAATAGTCTGAAAGCAATGCTGGCGTTCCGAGAAAAACTTCCTGCGTTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGACTGCAACATAATATGCACTCAACCTCGTCGTATATCTGCTGTGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACCACTGGAGTATTGCTTCGTCAGTTGGTTCAAGACCCACAGTTAACTGGCGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAATGAGGACTTTTTGTTAATAATTCTGCGTGACCTTCTTCCTCGACGTCCAGATCTGCGACTTATTCTAATGAGTGCTACCATTAATGCTGATTTGTTTTCCAAATACTTTGGAAATGCCCCAACAATACATATACCGGGATTAACGTTCCCTGTGGCAGAGCTATTTCTAGAAGATGTTTTGGAGAAAACTCGATACACAATTAAGTCAGAGTCTGACAAGATGGAGAGGGGAAATTCCAGGAGAAAGAGACAGCAAAACTCCAAGAAGGATCCCTTAATGGAACTATTTGAGGGTGTTGATATTGATCTTCGTTACAAAAGTTACAGCACATCTACAAGAAAGTCTCTAGAAGCTTGGTCTGGTTCACAACTTGATTTGGGTCTGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTGTTCCTTACGGGCTGGGATGATATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCTGAGAAGTTCATGGTTCTTCCCCTGCATGGATCAATGCCTACTGTCAATCAACGAGAAATATTTGACCGTCCTCCCGCTAACAAAAGAAAAATAGTGCTAGCAACCAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGTGGAAAGGCAAAGGAAACCAGTTACGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGCCGTGCTGGTCGTGTCCAACCTGGAGTTTGTTACAGACTATATCCAAAAATGATTCATGATGCAATGCTTCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCACATAAAAAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTGCAAAATGCCATTGAACTTCTCAAAACTATTGGAGCCTTAGATGATATGGAAGAGCTGACTCCACTTGGCCGCCATCTTTGCACTCTTCCATTGGATCCAAACATTGGAAAGATGCTTCTGATGGGTTCTGTCTTTCAGTGCCTCAATCCTGCCTTAACAATTGCTGCTGCCCTCGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGATCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACGTAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTTCAGTTTGTAGATCTATTATCAAACATTGGCTTTGTCGACAAATCCAAGGGTGCTAGTGCGTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTCGGTAAAGTTGATATTCATCCTGGATCAGTCAATGCTGGGGTTCATTTATTCCCTCTGCCTTACATGGTTTATAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAATTATATCAGATTATTCTTTGCTTTTATTTGGTGGTAATCTCATCCCAACCAAAACGGGAGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATACGGAAATTACGCCGTGAACTTGACAAGCTGTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTATTGAGGGAAAGGGAGTGGTATCTGCAGTCGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1038

Amino Acids

115.48

Weight (kDa)

8.96

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 276 - 430 3.2e-09 DEAD/DEAH box helicase
Helicase_C PF00271 535 - 662 1.5e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 726 - 754 1.9e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 755 - 814 2.4e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 885 - 965 3.4e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 975 - 1032 1.5e-20 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1249
Acc16I TGCGCA 1 cut(s) 26
AccB7I CCANNNNNTGG 3 cut(s) 1171, 2273, 2641
AccBSI CCGCTC 3 cut(s) 71, 93, 335
AccI GTMKAC 2 cut(s) 533, 2595
AccII CGCG 2 cut(s) 171, 207
AccIII TCCGGA 1 cut(s) 2064
AclI AACGTT 1 cut(s) 1346
AcoI YGGCCR 4 cut(s) 142, 178, 634, 2236
AcsI RAATTY 2 cut(s) 1435, 2058
AcuI CTGAAG 1 cut(s) 1935
AcyI GRCGYC 2 cut(s) 623, 1246
AfaI GTAC 3 cut(s) 285, 2621, 3108
AfeI AGCGCT 2 cut(s) 224, 1079
AfiI CCNNNNNNNGG 7 cut(s) 687, 1023, 1171, 1742, 2273, 2641, 3040
AgsI TTSAA 9 cut(s) 665, 734, 817, 1139, 1944, 2042, 2147, 2183, 2459
AjiI CACGTC 1 cut(s) 938
AjnI CCWGG 5 cut(s) 636, 686, 1439, 1990, 2758
Alw21I GWGCWC 1 cut(s) 575
AlwNI CAGNNNCTG 2 cut(s) 542, 1415
Ama87I CYCGRG 1 cut(s) 871
Aor13HI TCCGGA 1 cut(s) 2064
Aor51HI AGCGCT 2 cut(s) 224, 1079
AoxI GGCC 7 cut(s) 142, 157, 178, 399, 634, 1969, 2236
ApaI GGGCCC 1 cut(s) 161
ApeKI GCWGC 6 cut(s) 220, 1004, 2107, 2333, 2336, 2396
ApoI RAATTY 2 cut(s) 1435, 2058
AseI ATTAAT 1 cut(s) 1284
Asp700I GAANNNNTTC 1 cut(s) 804
AspLEI GCGC 4 cut(s) 27, 225, 360, 1080
AspS9I GGNCC 3 cut(s) 157, 158, 232
AsuC2I CCSGG 3 cut(s) 236, 482, 1338
AsuHPI GGTGA 6 cut(s) 227, 775, 887, 1052, 2429, 2514
AsuNHI GCTAGC 1 cut(s) 1813
AvaI CYCGRG 1 cut(s) 871
AvaII GGWCC 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 161
BalI TGGCCA 1 cut(s) 636
BamHI GGATCC 2 cut(s) 1471, 2263
BanII GRGCYC 2 cut(s) 161, 575
BbsI GAAGAC 1 cut(s) 2831
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 6 cut(s) 232, 991, 2119, 2320, 2323, 2383
BceAI ACGGC 4 cut(s) 154, 165, 1956, 2979
BciT130I CCWGG 5 cut(s) 638, 688, 1441, 1992, 2760
BclI TGATCA 2 cut(s) 376, 2431
BcnI CCSGG 3 cut(s) 236, 482, 1338
BfaI CTAG 6 cut(s) 410, 920, 1370, 1550, 1814, 2613
BfmI CTRYAG 3 cut(s) 534, 543, 3075
BfoI RGCGCY 2 cut(s) 226, 1081
BglII AGATCT 2 cut(s) 1252, 2569
BlpI GCTNAGC 1 cut(s) 249
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 8 cut(s) 236, 482, 638, 688, 1338, 1441, 1992, 2760
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 871
BmgBI CACGTC 1 cut(s) 938
BmgT120I GGNCC 3 cut(s) 157, 158, 232
BmiI GGNNCC 9 cut(s) 158, 159, 233, 234, 465, 508, 1473, 2203, 2265
BmrFI CCNGG 8 cut(s) 236, 482, 638, 688, 1338, 1441, 1992, 2760
BmsI GCATC 7 cut(s) 1891, 2020, 2271, 2383, 2461, 2919, 2966
BmtI GCTAGC 1 cut(s) 1817
BpiI GAAGAC 1 cut(s) 2831
BpmI CTGGAG 3 cut(s) 670, 1134, 2013
Bpu1102I GCTNAGC 1 cut(s) 249
BpuMI CCSGG 3 cut(s) 236, 482, 1338
BsaBI GATNNNNATC 3 cut(s) 350, 1506, 2754
BsaHI GRCGYC 2 cut(s) 623, 1246
BsaI GGTCTC 1 cut(s) 2346
BsaJI CCNNGG 5 cut(s) 136, 169, 637, 1705, 2604
BsaWI WCCGGW 1 cut(s) 2064
BsaXI ACNNNNNCTCC 2 cut(s) 2636, 2666
Bsc4I CCNNNNNNNGG 7 cut(s) 687, 1023, 1171, 1742, 2273, 2641, 3040
Bse1I ACTGG 5 cut(s) 1071, 1117, 1159, 1893, 2525
Bse3DI GCAATG 3 cut(s) 789, 1824, 2040
Bse8I GATNNNNATC 3 cut(s) 350, 1506, 2754
BseAI TCCGGA 1 cut(s) 2064
BseBI CCWGG 5 cut(s) 638, 688, 1441, 1992, 2760
BseDI CCNNGG 5 cut(s) 136, 169, 637, 1705, 2604
BseGI GGATG 7 cut(s) 86, 534, 1666, 2468, 2755, 2898, 3049
BseJI GATNNNNATC 3 cut(s) 350, 1506, 2754
BseLI CCNNNNNNNGG 7 cut(s) 687, 1023, 1171, 1742, 2273, 2641, 3040
BseMI GCAATG 3 cut(s) 789, 1824, 2040
BseMII CTCAG 8 cut(s) 117, 240, 248, 690, 819, 827, 923, 1707
BseNI ACTGG 5 cut(s) 1071, 1117, 1159, 1893, 2525
BseRI GAGGAG 2 cut(s) 71, 204
BseSI GKGCMC 1 cut(s) 161
BseX3I CGGCCG 2 cut(s) 142, 178
BseXI GCAGC 6 cut(s) 232, 991, 2119, 2320, 2323, 2383
BseYI CCCAGC 5 cut(s) 680, 1656, 2669, 2774, 3034
BsgI GTGCAG 1 cut(s) 2711
Bsh1236I CGCG 2 cut(s) 171, 207
Bsh1285I CGRYCG 3 cut(s) 145, 181, 205
BshFI GGCC 7 cut(s) 144, 159, 180, 401, 636, 1971, 2238
BsiEI CGRYCG 3 cut(s) 145, 181, 205
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 1 cut(s) 871
BsiSI CCGG 5 cut(s) 236, 473, 481, 1337, 2065
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 7 cut(s) 687, 1023, 1171, 1742, 2273, 2641, 3040
BsmBI CGTCTC 1 cut(s) 1957
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 2720
BsnI GGCC 7 cut(s) 144, 159, 180, 401, 636, 1971, 2238
Bso31I GGTCTC 1 cut(s) 2346
BsoBI CYCGRG 1 cut(s) 871
Bsp120I GGGCCC 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 161, 575
Bsp13I TCCGGA 1 cut(s) 2064
Bsp1407I TGTACA 1 cut(s) 3106
Bsp1720I GCTNAGC 1 cut(s) 249
BspANI GGCC 7 cut(s) 144, 159, 180, 401, 636, 1971, 2238
BspCNI CTCAG 8 cut(s) 116, 241, 249, 691, 820, 828, 922, 1708
BspEI TCCGGA 1 cut(s) 2064
BspFNI CGCG 2 cut(s) 171, 207
BspHI TCATGA 1 cut(s) 2026
BspLI GGNNCC 9 cut(s) 158, 159, 233, 234, 465, 508, 1473, 2203, 2265
BspMAI CTGCAG 2 cut(s) 547, 3079
BspOI GCTAGC 1 cut(s) 1817
BspQI GCTCTTC 1 cut(s) 2214
BspTNI GGTCTC 1 cut(s) 2346
BsrBI CCGCTC 3 cut(s) 71, 93, 335
BsrDI GCAATG 3 cut(s) 789, 1824, 2040
BsrGI TGTACA 1 cut(s) 3106
BsrI ACTGG 5 cut(s) 1071, 1117, 1159, 1893, 2525
BssECI CCNNGG 5 cut(s) 136, 169, 637, 1705, 2604
BssNI GRCGYC 2 cut(s) 623, 1246
BssT1I CCWWGG 2 cut(s) 1705, 2604
Bst2UI CCWGG 5 cut(s) 638, 688, 1441, 1992, 2760
Bst4CI ACNGT 7 cut(s) 706, 1048, 1149, 1600, 1759, 1784, 1874
Bst6I CTCTTC 3 cut(s) 2214, 2259, 2698
BstACI GRCGYC 2 cut(s) 623, 1246
BstAUI TGTACA 1 cut(s) 3106
BstC8I GCNNGC 8 cut(s) 40, 246, 575, 593, 597, 789, 1815, 2833
BstDSI CCRYGG 2 cut(s) 136, 169
BstEII GGTNACC 1 cut(s) 2939
BstENI CCTNNNNNAGG 1 cut(s) 1021
BstF5I GGATG 7 cut(s) 86, 534, 1666, 2468, 2755, 2898, 3049
BstFNI CGCG 2 cut(s) 171, 207
BstH2I RGCGCY 2 cut(s) 226, 1081
BstHHI GCGC 4 cut(s) 27, 225, 360, 1080
BstMCI CGRYCG 3 cut(s) 145, 181, 205
BstNI CCWGG 5 cut(s) 638, 688, 1441, 1992, 2760
BstPI GGTNACC 1 cut(s) 2939
BstSCI CCNGG 8 cut(s) 234, 480, 636, 686, 1336, 1439, 1990, 2758
BstSFI CTRYAG 3 cut(s) 534, 543, 3075
BstSLI GKGCMC 1 cut(s) 161
BstUI CGCG 2 cut(s) 171, 207
BstV1I GCAGC 6 cut(s) 232, 991, 2119, 2320, 2323, 2383
BstV2I GAAGAC 1 cut(s) 2831
BstX2I RGATCY 6 cut(s) 1252, 1471, 1711, 2263, 2405, 2569
BstYI RGATCY 6 cut(s) 1252, 1471, 1711, 2263, 2405, 2569
BstZI CGGCCG 2 cut(s) 142, 178
BsuRI GGCC 7 cut(s) 144, 159, 180, 401, 636, 1971, 2238
BtgI CCRYGG 2 cut(s) 136, 169
BtrI CACGTC 1 cut(s) 938
BtsCI GGATG 7 cut(s) 86, 534, 1666, 2468, 2755, 2898, 3049
BtsI GCAGTG 2 cut(s) 2172, 2699
BtsIMutI CAGTG 5 cut(s) 1110, 2172, 2312, 2434, 2699
Cac8I GCNNGC 8 cut(s) 40, 246, 575, 593, 597, 789, 1815, 2833
CaiI CAGNNNCTG 2 cut(s) 542, 1415
CciI TCATGA 1 cut(s) 2026
CciNI GCGGCCGC 1 cut(s) 142
CfoI GCGC 4 cut(s) 27, 225, 360, 1080
Cfr13I GGNCC 3 cut(s) 157, 158, 232
Cfr42I CCGCGG 1 cut(s) 172
CseI GACGC 1 cut(s) 612
Csp6I GTAC 3 cut(s) 284, 2620, 3107
CviQI GTAC 3 cut(s) 284, 2620, 3107
DraI TTTAAA 2 cut(s) 325, 1908
EaeI YGGCCR 4 cut(s) 142, 178, 634, 2236
EagI CGGCCG 2 cut(s) 142, 178
Eam1104I CTCTTC 3 cut(s) 2214, 2259, 2698
EarI CTCTTC 3 cut(s) 2214, 2259, 2698
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 573
EclXI CGGCCG 2 cut(s) 142, 178
Eco130I CCWWGG 2 cut(s) 1705, 2604
Eco24I GRGCYC 2 cut(s) 161, 575
Eco31I GGTCTC 1 cut(s) 2346
Eco47I GGWCC 1 cut(s) 232
Eco47III AGCGCT 2 cut(s) 224, 1079
Eco52I CGGCCG 2 cut(s) 142, 178
Eco53kI GAGCTC 1 cut(s) 573
Eco57I CTGAAG 1 cut(s) 1935
Eco88I CYCGRG 1 cut(s) 871
Eco91I GGTNACC 1 cut(s) 2939
EcoICRI GAGCTC 1 cut(s) 573
EcoNI CCTNNNNNAGG 1 cut(s) 1021
EcoO109I RGGNCCY 3 cut(s) 157, 158, 232
EcoO65I GGTNACC 1 cut(s) 2939
EcoRII CCWGG 5 cut(s) 636, 686, 1439, 1990, 2758
EcoT14I CCWWGG 2 cut(s) 1705, 2604
EcoT38I GRGCYC 2 cut(s) 161, 575
ErhI CCWWGG 2 cut(s) 1705, 2604
Esp3I CGTCTC 1 cut(s) 1957
FalI AAGNNNNNCTT 4 cut(s) 708, 740, 1460, 1492
FaqI GGGAC 1 cut(s) 245
FauI CCCGC 4 cut(s) 47, 98, 176, 1798
FbaI TGATCA 2 cut(s) 376, 2431
FblI GTMKAC 2 cut(s) 533, 2595
FokI GGATG 7 cut(s) 73, 541, 1673, 2475, 2742, 2885, 3056
FriOI GRGCYC 2 cut(s) 161, 575
FspBI CTAG 6 cut(s) 410, 920, 1370, 1550, 1814, 2613
FspI TGCGCA 1 cut(s) 26
GlaI GCGC 4 cut(s) 26, 224, 359, 1079
GsaI CCCAGC 5 cut(s) 684, 1660, 2673, 2778, 3038
GsuI CTGGAG 3 cut(s) 670, 1134, 2013
HaeII RGCGCY 2 cut(s) 226, 1081
HaeIII GGCC 7 cut(s) 144, 159, 180, 401, 636, 1971, 2238
HapII CCGG 5 cut(s) 236, 473, 481, 1337, 2065
HgaI GACGC 1 cut(s) 612
HhaI GCGC 4 cut(s) 27, 225, 360, 1080
Hin1I GRCGYC 2 cut(s) 623, 1246
Hin6I GCGC 4 cut(s) 25, 223, 358, 1078
HinP1I GCGC 4 cut(s) 25, 223, 358, 1078
HincII GTYRAC 2 cut(s) 1152, 2596
HindII GTYRAC 2 cut(s) 1152, 2596
HindIII AAGCTT 3 cut(s) 597, 1554, 2451
HpaI GTTAAC 1 cut(s) 1152
HpaII CCGG 5 cut(s) 236, 473, 481, 1337, 2065
HphI GGTGA 6 cut(s) 227, 775, 887, 1052, 2429, 2514
Hpy166II GTNNAC 6 cut(s) 534, 1152, 1568, 2596, 2999, 3109
Hpy8I GTNNAC 6 cut(s) 534, 1152, 1568, 2596, 2999, 3109
Hpy99I CGWCG 3 cut(s) 186, 984, 1248
HpyAV CCTTC 8 cut(s) 109, 436, 740, 754, 1244, 1462, 2453, 2954
HpyCH4III ACNGT 7 cut(s) 706, 1048, 1149, 1600, 1759, 1784, 1874
HpyCH4IV ACGT 4 cut(s) 937, 1246, 1346, 2479
HpySE526I ACGT 4 cut(s) 937, 1246, 1346, 2479
Hsp92I GRCGYC 2 cut(s) 623, 1246
HspAI GCGC 4 cut(s) 25, 223, 358, 1078
KflI GGGWCCC 1 cut(s) 232
Kpn2I TCCGGA 1 cut(s) 2064
Ksp22I TGATCA 2 cut(s) 376, 2431
KspAI GTTAAC 1 cut(s) 1152
KspI CCGCGG 1 cut(s) 172
LguI GCTCTTC 1 cut(s) 2214
LmnI GCTCC 9 cut(s) 76, 98, 316, 332, 337, 689, 1631, 2114, 2201
Lsp1109I GCAGC 6 cut(s) 232, 991, 2119, 2320, 2323, 2383
LweI GCATC 7 cut(s) 1891, 2020, 2271, 2383, 2461, 2919, 2966
MaeI CTAG 6 cut(s) 410, 920, 1370, 1550, 1814, 2613
MaeII ACGT 4 cut(s) 937, 1246, 1346, 2479
MaeIII GTNAC 9 cut(s) 1229, 1514, 1523, 1674, 1895, 2000, 2049, 2527, 2939
MbiI CCGCTC 3 cut(s) 71, 93, 335
MfeI CAATTG 1 cut(s) 2328
MflI RGATCY 6 cut(s) 1252, 1471, 1711, 2263, 2405, 2569
MhlI GDGCHC 2 cut(s) 161, 575
MlsI TGGCCA 1 cut(s) 636
MluNI TGGCCA 1 cut(s) 636
MlyI GAGTC 5 cut(s) 72, 568, 1421, 2221, 2843
MmeI TCCRAC 4 cut(s) 144, 672, 2011, 2101
Mox20I TGGCCA 1 cut(s) 636
MroI TCCGGA 1 cut(s) 2064
MroXI GAANNNNTTC 1 cut(s) 804
MscI TGGCCA 1 cut(s) 636
MslI CAYNNNNRTG 2 cut(s) 423, 2927
Msp20I TGGCCA 1 cut(s) 636
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 5 cut(s) 236, 473, 481, 1337, 2065
MspR9I CCNGG 8 cut(s) 236, 482, 638, 688, 1338, 1441, 1992, 2760
MunI CAATTG 1 cut(s) 2328
Mva1269I GAATGC 1 cut(s) 2720
MvaI CCWGG 5 cut(s) 638, 688, 1441, 1992, 2760
MvnI CGCG 2 cut(s) 171, 207
NciI CCSGG 3 cut(s) 236, 482, 1338
NheI GCTAGC 1 cut(s) 1813
NlaIV GGNNCC 9 cut(s) 158, 159, 233, 234, 465, 508, 1473, 2203, 2265
NmuCI GTSAC 1 cut(s) 1229
NotI GCGGCCGC 1 cut(s) 142
NsbI TGCGCA 1 cut(s) 26
PagI TCATGA 1 cut(s) 2026
PciSI GCTCTTC 1 cut(s) 2214
PctI GAATGC 1 cut(s) 2720
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 9 cut(s) 290, 523, 1033, 1060, 1070, 1701, 2023, 2156, 2420
PflFI GACNNNGTC 1 cut(s) 236
PflMI CCANNNNNTGG 3 cut(s) 1171, 2273, 2641
PfoI TCCNGGA 2 cut(s) 1439, 2758
PleI GAGTC 5 cut(s) 72, 567, 1420, 2221, 2843
PpsI GAGTC 5 cut(s) 72, 567, 1420, 2221, 2843
PpuMI RGGWCCY 1 cut(s) 232
PshBI ATTAAT 1 cut(s) 1284
Psp124BI GAGCTC 1 cut(s) 575
Psp1406I AACGTT 1 cut(s) 1346
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 5 cut(s) 636, 686, 1439, 1990, 2758
PspEI GGTNACC 1 cut(s) 2939
PspFI CCCAGC 5 cut(s) 680, 1656, 2669, 2774, 3034
PspGI CCWGG 5 cut(s) 636, 686, 1439, 1990, 2758
PspN4I GGNNCC 9 cut(s) 158, 159, 233, 234, 465, 508, 1473, 2203, 2265
PspOMI GGGCCC 1 cut(s) 157
PspPI GGNCC 3 cut(s) 157, 158, 232
PspPPI RGGWCCY 1 cut(s) 232
PstI CTGCAG 2 cut(s) 547, 3079
PstNI CAGNNNCTG 2 cut(s) 542, 1415
PsuI RGATCY 6 cut(s) 1252, 1471, 1711, 2263, 2405, 2569
PsyI GACNNNGTC 1 cut(s) 236
RsaI GTAC 3 cut(s) 285, 2621, 3108
RsaNI GTAC 3 cut(s) 284, 2620, 3107
RseI CAYNNNNRTG 2 cut(s) 423, 2927
SacI GAGCTC 1 cut(s) 575
SacII CCGCGG 1 cut(s) 172
SalI GTCGAC 1 cut(s) 2594
SapI GCTCTTC 1 cut(s) 2214
Sau96I GGNCC 3 cut(s) 157, 158, 232
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 5 cut(s) 72, 568, 1421, 2221, 2843
ScrFI CCNGG 8 cut(s) 236, 482, 638, 688, 1338, 1441, 1992, 2760
SduI GDGCHC 2 cut(s) 161, 575
SfaNI GCATC 7 cut(s) 1891, 2020, 2271, 2383, 2461, 2919, 2966
SfcI CTRYAG 3 cut(s) 534, 543, 3075
Sfr303I CCGCGG 1 cut(s) 172
SgrBI CCGCGG 1 cut(s) 172
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 423, 2927
SspI AATATT 1 cut(s) 1776
SspMI CTAG 6 cut(s) 410, 920, 1370, 1550, 1814, 2613
SstI GAGCTC 1 cut(s) 575
StyD4I CCNGG 8 cut(s) 234, 480, 636, 686, 1336, 1439, 1990, 2758
StyI CCWWGG 2 cut(s) 1705, 2604
TaaI ACNGT 7 cut(s) 706, 1048, 1149, 1600, 1759, 1784, 1874
TaiI ACGT 4 cut(s) 940, 1249, 1349, 2482
TaqI TCGA 6 cut(s) 293, 1063, 1243, 1393, 2350, 2595
TatI WGTACW 2 cut(s) 283, 3106
TauI GCSGC 8 cut(s) 71, 144, 147, 174, 177, 180, 272, 2241
TfiI GAWTC 9 cut(s) 290, 523, 1033, 1060, 1070, 1701, 2023, 2156, 2420
TscAI CASTG 5 cut(s) 1117, 2172, 2312, 2434, 2699
TseFI GTSAC 1 cut(s) 1229
TseI GCWGC 6 cut(s) 220, 1004, 2107, 2333, 2336, 2396
Tsp45I GTSAC 1 cut(s) 1229
TspGWI ACGGA 2 cut(s) 456, 2998
TspRI CASTG 5 cut(s) 1117, 2172, 2312, 2434, 2699
Tth111I GACNNNGTC 1 cut(s) 236
Van91I CCANNNNNTGG 3 cut(s) 1171, 2273, 2641
VpaK11BI GGWCC 1 cut(s) 232
VspI ATTAAT 1 cut(s) 1284
XagI CCTNNNNNAGG 1 cut(s) 1021
XapI RAATTY 2 cut(s) 1435, 2058
XbaI TCTAGA 3 cut(s) 919, 1369, 1549
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmiI GTMKAC 2 cut(s) 533, 2595
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 6 cut(s) 410, 920, 1370, 1550, 1814, 2613
ZraI GACGTC 1 cut(s) 1247
ZrmI AGTACT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.