RLG00000023781

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007834
Physical Location & Seq
Forward (+)
28118607 .. 28127491
8885 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023781

Sequence Viewer

Length: 3033 bp
ATGTCCAGCCGCCCCAACTTCCAGGGCGGCCGCAGCAGAGGCGGCCCTAACTCCCGCGGCGGCGCCCGCCGTGGAGGCGGTGGTCGCGGCGGTGGAGGCCGAGGAGGGGGGCGTGGCGGCGGCGGCCGAGGCGGCGAACAGCGCTGGTGGGACCCCGTGTGGCGAGCCGAGCGACTGAGGCAACAAGCCGCGGAGATGGAAGTTCTTGATGCGAGTGAGTACTGGGGGAAGATGGAGCAGTTCAGAAGCGGAGCGGAGCAGGAGATGATAATAAGACGTAATTTTAGTCGGCATGATCAGCAAACCCTATATGAAATGGCCTATGAACTAGGGCTTCACTTCCATGCATATAATAAGGGGAAGGCTCTTGTGGTCAGCAAGGTTCCTCTGCCAGATTATCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGAATGTCTACAGAGACTGCAGAGAGAGTCGGGAGTCTTTTGCAAAGCTCGCAGGGGCAGGGAAGTAAGCAAGCTTTTGGTAACGTTGCTTCAGTGGCGTCTGGGTCTGTCCAGGGGAACAAACTAACGACTGTTAGTGAAAATACAACCAAACCCAGCTCCAGCTTGGAGCCTGATACTGTTAAGGAGAAAGAGAAACTCAGCCTTCAACTTAAGGAGTCACAGGAAAAAATGAAGGTAAGTAATAGTCTGAAATCAATGCTGACGTTCCGAGAAAAGCTTCCTGCATTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGAGTGCAACATAATATGCACTCAGCCTCGCCGTATATCTGCCGTGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACAACTGGAGTATTACTTCGTCAGTTGGTTCAAGACCCACAGTTAACTGGCGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAACGAGGACTTTTTACTAATAATTTTGCGAGACCTTCTTCCTCGACGTCCAGATCTACGACTTATTCTAATGAGTGCTACCATCAATGCTGACTTGTTTTCCAAATACTTTGGAAATACCCCAACAATACATATACCGGGATTAACGTTCCCTGTGGCAGAGCTATTTCTAGAAGACGTTTTGGAGAAAACTCATTATATAGTCAAGTCAGAGTCCGACAAGATGGAGAGGGGAAATTCCAGGAGAAGGAGACAGCAAGACTCCAAGAAGGATCCCTTAACTGAACTATTTGAGGATGTTGATATTGATCTTCACTACAAAAGTTACAGCACATCTACAAGAAAGTCTCTAGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTCTGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACGGGCTGGGATGATATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCTGAGAAGTTCATGGTTCTTCCCCTGCATGGGTCAATGCCTACTATCAATCAACGAGAAATATTTGATCGTCCTCCCACTAACAAAAGAAAAATTGTGCTAGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGCGGAAAGGCAAAGGAAACGAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATATCCAAAAATGATTCACGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCGAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTCTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCCTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCTAAAAGATCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGCATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTTGACAAATCCAGGGGTGCTAACGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTTGGTAAAGTCGATATCCATCCTGGATCAGTCAATGCTGGGGTTCACTTATTCCCTCTGCCTTACATGGTATATAGTGAAAAAGTGAAGACAGCCAGCATTTACATTAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTTGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTAGTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1011

Amino Acids

112.34

Weight (kDa)

8.72

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 249 - 403 2.9e-09 DEAD/DEAH box helicase
Helicase_C PF00271 508 - 635 1e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 699 - 727 1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 728 - 787 2.3e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 858 - 938 9.8e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 948 - 1005 1.3e-21 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1168
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 3 cut(s) 1090, 2192, 2560
AccBSI CCGCTC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 452
AccII CGCG 3 cut(s) 57, 87, 191
AccIII TCCGGA 1 cut(s) 1983
AclI AACGTT 2 cut(s) 528, 1265
AclWI GGATC 9 cut(s) 1385, 1398, 1625, 2050, 2177, 2190, 2319, 2413, 2689
AcoI YGGCCR 3 cut(s) 28, 124, 1897
AcsI RAATTY 2 cut(s) 1354, 1977
AcuI CTGAAG 2 cut(s) 519, 1854
AcyI GRCGYC 3 cut(s) 63, 542, 1165
AfaI GTAC 3 cut(s) 221, 2170, 3027
AfeI AGCGCT 2 cut(s) 143, 998
AfiI CCNNNNNNNGG 9 cut(s) 106, 942, 1090, 1365, 1661, 1662, 2192, 2560, 2959
AflII CTTAAG 1 cut(s) 656
AgsI TTSAA 8 cut(s) 653, 736, 1058, 1863, 2066, 2090, 2225, 2378
AjiI CACGTC 1 cut(s) 857
AjnI CCWGG 6 cut(s) 21, 555, 1358, 1909, 2522, 2677
AlwI GGATC 9 cut(s) 1385, 1398, 1625, 2050, 2177, 2190, 2319, 2413, 2689
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 790
Aor13HI TCCGGA 1 cut(s) 1983
Aor51HI AGCGCT 2 cut(s) 143, 998
AoxI GGCC 6 cut(s) 28, 43, 97, 124, 318, 1897
ApeKI GCWGC 6 cut(s) 33, 923, 2026, 2252, 2255, 2315
ApoI RAATTY 2 cut(s) 1354, 1977
Asp700I GAANNNNTTC 1 cut(s) 723
AspLEI GCGC 4 cut(s) 65, 144, 999, 2913
AspS9I GGNCC 2 cut(s) 44, 151
AsuC2I CCSGG 1 cut(s) 1257
AsuHPI GGTGA 4 cut(s) 806, 971, 2348, 2433
AsuII TTCGAA 1 cut(s) 445
AsuNHI GCTAGC 1 cut(s) 1732
AvaI CYCGRG 1 cut(s) 790
AvaII GGWCC 1 cut(s) 151
BamHI GGATCC 2 cut(s) 1390, 2182
BanI GGYRCC 1 cut(s) 62
BarI GAAGNNNNNNTAC 2 cut(s) 517, 549
BbsI GAAGAC 2 cut(s) 1299, 2750
BbvI GCAGC 6 cut(s) 45, 910, 2038, 2239, 2242, 2302
BceAI ACGGC 5 cut(s) 54, 885, 896, 1884, 2898
BciT130I CCWGG 6 cut(s) 23, 557, 1360, 1911, 2524, 2679
BclI TGATCA 2 cut(s) 295, 2350
BcnI CCSGG 1 cut(s) 1257
BfaI CTAG 7 cut(s) 329, 839, 1289, 1469, 1733, 2261, 2970
BfmI CTRYAG 2 cut(s) 453, 462
BfoI RGCGCY 3 cut(s) 66, 145, 1000
BfrI CTTAAG 1 cut(s) 656
BglI GCCNNNNNGGC 2 cut(s) 75, 132
BglII AGATCT 2 cut(s) 1171, 2488
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 7 cut(s) 23, 557, 1257, 1360, 1911, 2524, 2679
Bme18I GGWCC 1 cut(s) 151
BmeT110I CYCGRG 1 cut(s) 790
BmgBI CACGTC 1 cut(s) 857
BmgT120I GGNCC 2 cut(s) 44, 151
BmiI GGNNCC 8 cut(s) 64, 152, 153, 384, 427, 615, 1392, 2184
BmrFI CCNGG 7 cut(s) 23, 557, 1257, 1360, 1911, 2524, 2679
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 8 cut(s) 199, 1810, 1939, 2190, 2302, 2380, 2838, 2885
BmtI GCTAGC 1 cut(s) 1736
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 2 cut(s) 1299, 2750
BpmI CTGGAG 4 cut(s) 589, 1053, 1932, 2853
Bpu14I TTCGAA 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 1257
BsaBI GATNNNNATC 2 cut(s) 1425, 2673
BsaHI GRCGYC 3 cut(s) 63, 542, 1165
BsaI GGTCTC 2 cut(s) 1143, 2265
BsaJI CCNNGG 9 cut(s) 22, 55, 70, 100, 127, 189, 556, 1624, 2523
BsaWI WCCGGW 1 cut(s) 1983
BsaXI ACNNNNNCTCC 2 cut(s) 2555, 2585
Bsc4I CCNNNNNNNGG 9 cut(s) 106, 942, 1090, 1365, 1661, 1662, 2192, 2560, 2959
Bse1I ACTGG 6 cut(s) 227, 990, 1036, 1078, 2444, 2836
Bse3DI GCAATG 2 cut(s) 1743, 1959
Bse8I GATNNNNATC 2 cut(s) 1425, 2673
BseAI TCCGGA 1 cut(s) 1983
BseBI CCWGG 6 cut(s) 23, 557, 1360, 1911, 2524, 2679
BseDI CCNNGG 9 cut(s) 22, 55, 70, 100, 127, 189, 556, 1624, 2523
BseGI GGATG 4 cut(s) 1420, 1585, 2674, 2968
BseJI GATNNNNATC 2 cut(s) 1425, 2673
BseLI CCNNNNNNNGG 9 cut(s) 106, 942, 1090, 1365, 1661, 1662, 2192, 2560, 2959
BseMI GCAATG 2 cut(s) 1743, 1959
BseMII CTCAG 8 cut(s) 167, 658, 738, 746, 842, 861, 905, 1626
BseNI ACTGG 6 cut(s) 227, 990, 1036, 1078, 2444, 2836
BseRI GAGGAG 2 cut(s) 117, 2153
BseX3I CGGCCG 2 cut(s) 28, 124
BseXI GCAGC 6 cut(s) 45, 910, 2038, 2239, 2242, 2302
BseYI CCCAGC 5 cut(s) 599, 1575, 2588, 2693, 2953
BsgI GTGCAG 1 cut(s) 2630
Bsh1236I CGCG 3 cut(s) 57, 87, 191
Bsh1285I CGRYCG 2 cut(s) 31, 127
BshFI GGCC 6 cut(s) 30, 45, 99, 126, 320, 1899
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 2 cut(s) 31, 127
BsiHKCI CYCGRG 1 cut(s) 790
BsiSI CCGG 2 cut(s) 1256, 1984
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 9 cut(s) 106, 942, 1090, 1365, 1661, 1662, 2192, 2560, 2959
BsmBI CGTCTC 1 cut(s) 1876
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 2 cut(s) 731, 2639
BsnI GGCC 6 cut(s) 30, 45, 99, 126, 320, 1899
Bso31I GGTCTC 2 cut(s) 1143, 2265
BsoBI CYCGRG 1 cut(s) 790
Bsp119I TTCGAA 1 cut(s) 445
Bsp13I TCCGGA 1 cut(s) 1983
Bsp1407I TGTACA 2 cut(s) 2168, 3025
BspANI GGCC 6 cut(s) 30, 45, 99, 126, 320, 1899
BspCNI CTCAG 8 cut(s) 168, 657, 739, 747, 841, 862, 904, 1627
BspEI TCCGGA 1 cut(s) 1983
BspFNI CGCG 3 cut(s) 57, 87, 191
BspLI GGNNCC 8 cut(s) 64, 152, 153, 384, 427, 615, 1392, 2184
BspMAI CTGCAG 1 cut(s) 466
BspOI GCTAGC 1 cut(s) 1736
BspPI GGATC 9 cut(s) 1385, 1398, 1625, 2050, 2177, 2190, 2319, 2413, 2689
BspT104I TTCGAA 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 656
BspTNI GGTCTC 2 cut(s) 1143, 2265
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 2 cut(s) 1743, 1959
BsrGI TGTACA 2 cut(s) 2168, 3025
BsrI ACTGG 6 cut(s) 227, 990, 1036, 1078, 2444, 2836
BssECI CCNNGG 9 cut(s) 22, 55, 70, 100, 127, 189, 556, 1624, 2523
BssNI GRCGYC 3 cut(s) 63, 542, 1165
BssT1I CCWWGG 1 cut(s) 1624
Bst2UI CCWGG 6 cut(s) 23, 557, 1360, 1911, 2524, 2679
Bst4CI ACNGT 5 cut(s) 577, 625, 967, 1068, 1519
Bst6I CTCTTC 1 cut(s) 2617
BstACI GRCGYC 3 cut(s) 63, 542, 1165
BstAFI CTTAAG 1 cut(s) 656
BstAUI TGTACA 2 cut(s) 2168, 3025
BstBI TTCGAA 1 cut(s) 445
BstC8I GCNNGC 8 cut(s) 67, 165, 494, 516, 1734, 1893, 1897, 2752
BstDEI CTNAG 8 cut(s) 176, 644, 747, 755, 828, 870, 891, 1635
BstDSI CCRYGG 3 cut(s) 55, 70, 189
BstEII GGTNACC 1 cut(s) 2858
BstENI CCTNNNNNAGG 1 cut(s) 940
BstF5I GGATG 4 cut(s) 1420, 1585, 2674, 2968
BstFNI CGCG 3 cut(s) 57, 87, 191
BstH2I RGCGCY 3 cut(s) 66, 145, 1000
BstHHI GCGC 4 cut(s) 65, 144, 999, 2913
BstMCI CGRYCG 2 cut(s) 31, 127
BstNI CCWGG 6 cut(s) 23, 557, 1360, 1911, 2524, 2679
BstPI GGTNACC 1 cut(s) 2858
BstSCI CCNGG 7 cut(s) 21, 555, 1255, 1358, 1909, 2522, 2677
BstSFI CTRYAG 2 cut(s) 453, 462
BstUI CGCG 3 cut(s) 57, 87, 191
BstV1I GCAGC 6 cut(s) 45, 910, 2038, 2239, 2242, 2302
BstV2I GAAGAC 2 cut(s) 1299, 2750
BstX2I RGATCY 6 cut(s) 1171, 1390, 1630, 2182, 2324, 2488
BstYI RGATCY 6 cut(s) 1171, 1390, 1630, 2182, 2324, 2488
BstZI CGGCCG 2 cut(s) 28, 124
BsuRI GGCC 6 cut(s) 30, 45, 99, 126, 320, 1899
BtgI CCRYGG 3 cut(s) 55, 70, 189
BtrI CACGTC 1 cut(s) 857
BtsCI GGATG 4 cut(s) 1420, 1585, 2674, 2968
BtsI GCAGTG 1 cut(s) 2618
BtsIMutI CAGTG 3 cut(s) 543, 2353, 2618
Cac8I GCNNGC 8 cut(s) 67, 165, 494, 516, 1734, 1893, 1897, 2752
CaiI CAGNNNCTG 1 cut(s) 461
CciNI GCGGCCGC 1 cut(s) 28
CfoI GCGC 4 cut(s) 65, 144, 999, 2913
Cfr13I GGNCC 2 cut(s) 44, 151
Cfr42I CCGCGG 2 cut(s) 58, 192
CseI GACGC 1 cut(s) 531
Csp6I GTAC 3 cut(s) 220, 2169, 3026
CviQI GTAC 3 cut(s) 220, 2169, 3026
DdeI CTNAG 8 cut(s) 176, 644, 747, 755, 828, 870, 891, 1635
DinI GGCGCC 1 cut(s) 64
DraI TTTAAA 2 cut(s) 1827, 2934
EaeI YGGCCR 3 cut(s) 28, 124, 1897
EagI CGGCCG 2 cut(s) 28, 124
Eam1104I CTCTTC 1 cut(s) 2617
EarI CTCTTC 1 cut(s) 2617
EclXI CGGCCG 2 cut(s) 28, 124
Eco130I CCWWGG 1 cut(s) 1624
Eco31I GGTCTC 2 cut(s) 1143, 2265
Eco32I GATATC 1 cut(s) 2671
Eco47I GGWCC 1 cut(s) 151
Eco47III AGCGCT 2 cut(s) 143, 998
Eco52I CGGCCG 2 cut(s) 28, 124
Eco57I CTGAAG 2 cut(s) 519, 1854
Eco88I CYCGRG 1 cut(s) 790
Eco91I GGTNACC 1 cut(s) 2858
EcoNI CCTNNNNNAGG 1 cut(s) 940
EcoO109I RGGNCCY 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 2858
EcoRII CCWGG 6 cut(s) 21, 555, 1358, 1909, 2522, 2677
EcoRV GATATC 1 cut(s) 2671
EcoT14I CCWWGG 1 cut(s) 1624
EcoT22I ATGCAT 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 1624
Esp3I CGTCTC 1 cut(s) 1876
FalI AAGNNNNNCTT 4 cut(s) 1379, 1411, 2189, 2221
FaqI GGGAC 1 cut(s) 164
FauI CCCGC 2 cut(s) 62, 74
FbaI TGATCA 2 cut(s) 295, 2350
FblI GTMKAC 1 cut(s) 452
FokI GGATG 4 cut(s) 1427, 1592, 2661, 2975
FspBI CTAG 7 cut(s) 329, 839, 1289, 1469, 1733, 2261, 2970
GlaI GCGC 4 cut(s) 64, 143, 998, 2912
GsaI CCCAGC 5 cut(s) 603, 1579, 2592, 2697, 2957
GsuI CTGGAG 4 cut(s) 589, 1053, 1932, 2853
HaeII RGCGCY 3 cut(s) 66, 145, 1000
HaeIII GGCC 6 cut(s) 30, 45, 99, 126, 320, 1899
HapII CCGG 2 cut(s) 1256, 1984
HgaI GACGC 1 cut(s) 531
HhaI GCGC 4 cut(s) 65, 144, 999, 2913
Hin1I GRCGYC 3 cut(s) 63, 542, 1165
Hin6I GCGC 4 cut(s) 63, 142, 997, 2911
HinP1I GCGC 4 cut(s) 63, 142, 997, 2911
HincII GTYRAC 2 cut(s) 1071, 2515
HindII GTYRAC 2 cut(s) 1071, 2515
HindIII AAGCTT 5 cut(s) 516, 722, 1473, 2370, 2927
HpaI GTTAAC 1 cut(s) 1071
HpaII CCGG 2 cut(s) 1256, 1984
HphI GGTGA 4 cut(s) 806, 971, 2348, 2433
Hpy166II GTNNAC 8 cut(s) 453, 1071, 1487, 2171, 2515, 2701, 2918, 3028
Hpy8I GTNNAC 8 cut(s) 453, 1071, 1487, 2171, 2515, 2701, 2918, 3028
Hpy99I CGWCG 1 cut(s) 1167
HpyAV CCTTC 8 cut(s) 355, 659, 673, 1163, 1359, 1381, 2050, 2873
HpyCH4III ACNGT 5 cut(s) 577, 625, 967, 1068, 1519
HpyCH4IV ACGT 7 cut(s) 277, 528, 710, 856, 1165, 1265, 1296
HpyF3I CTNAG 8 cut(s) 176, 644, 747, 755, 828, 870, 891, 1635
HpySE526I ACGT 7 cut(s) 277, 528, 710, 856, 1165, 1265, 1296
Hsp92I GRCGYC 3 cut(s) 63, 542, 1165
HspAI GCGC 4 cut(s) 63, 142, 997, 2911
KasI GGCGCC 1 cut(s) 62
KflI GGGWCCC 1 cut(s) 151
Kpn2I TCCGGA 1 cut(s) 1983
Ksp22I TGATCA 2 cut(s) 295, 2350
KspAI GTTAAC 1 cut(s) 1071
KspI CCGCGG 2 cut(s) 58, 192
Lsp1109I GCAGC 6 cut(s) 45, 910, 2038, 2239, 2242, 2302
LweI GCATC 8 cut(s) 199, 1810, 1939, 2190, 2302, 2380, 2838, 2885
MaeI CTAG 7 cut(s) 329, 839, 1289, 1469, 1733, 2261, 2970
MaeII ACGT 7 cut(s) 277, 528, 710, 856, 1165, 1265, 1296
MaeIII GTNAC 8 cut(s) 524, 663, 1442, 1593, 1919, 1968, 2446, 2858
MbiI CCGCTC 1 cut(s) 254
MboII GAAGA 9 cut(s) 241, 854, 1148, 1304, 1421, 1643, 2634, 2755, 2953
MfeI CAATTG 1 cut(s) 2247
MflI RGATCY 6 cut(s) 1171, 1390, 1630, 2182, 2324, 2488
MluCI AATT 9 cut(s) 280, 1140, 1354, 1605, 1725, 1977, 2247, 2577, 2906
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 6 cut(s) 480, 487, 671, 1340, 1373, 2762
MmeI TCCRAC 4 cut(s) 1359, 1930, 2020, 2846
Mph1103I ATGCAT 1 cut(s) 349
MroI TCCGGA 1 cut(s) 1983
MroXI GAANNNNTTC 1 cut(s) 723
MslI CAYNNNNRTG 2 cut(s) 342, 2846
MspA1I CMGCKG 2 cut(s) 57, 191
MspCI CTTAAG 1 cut(s) 656
MspI CCGG 2 cut(s) 1256, 1984
MspR9I CCNGG 7 cut(s) 23, 557, 1257, 1360, 1911, 2524, 2679
MunI CAATTG 1 cut(s) 2247
Mva1269I GAATGC 2 cut(s) 731, 2639
MvaI CCWGG 6 cut(s) 23, 557, 1360, 1911, 2524, 2679
MvnI CGCG 3 cut(s) 57, 87, 191
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 1257
NheI GCTAGC 1 cut(s) 1732
NlaIV GGNNCC 8 cut(s) 64, 152, 153, 384, 427, 615, 1392, 2184
NmeAIII GCCGAG 3 cut(s) 125, 152, 193
NmuCI GTSAC 1 cut(s) 663
NotI GCGGCCGC 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 349
NspV TTCGAA 1 cut(s) 445
PctI GAATGC 2 cut(s) 731, 2639
PdmI GAANNNNTTC 1 cut(s) 723
PfeI GAWTC 9 cut(s) 442, 952, 979, 989, 1620, 1942, 2075, 2339, 2899
PflMI CCANNNNNTGG 3 cut(s) 1090, 2192, 2560
PfoI TCCNGGA 2 cut(s) 1358, 2677
PleI GAGTC 6 cut(s) 479, 486, 670, 1339, 1373, 2762
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 6 cut(s) 479, 486, 670, 1339, 1373, 2762
PpuMI RGGWCCY 1 cut(s) 151
Psp1406I AACGTT 2 cut(s) 528, 1265
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 6 cut(s) 21, 555, 1358, 1909, 2522, 2677
PspEI GGTNACC 1 cut(s) 2858
PspFI CCCAGC 5 cut(s) 599, 1575, 2588, 2693, 2953
PspGI CCWGG 6 cut(s) 21, 555, 1358, 1909, 2522, 2677
PspN4I GGNNCC 8 cut(s) 64, 152, 153, 384, 427, 615, 1392, 2184
PspPI GGNCC 2 cut(s) 44, 151
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 6 cut(s) 1171, 1390, 1630, 2182, 2324, 2488
RsaI GTAC 3 cut(s) 221, 2170, 3027
RsaNI GTAC 3 cut(s) 220, 2169, 3026
RseI CAYNNNNRTG 2 cut(s) 342, 2846
SacII CCGCGG 2 cut(s) 58, 192
Sau96I GGNCC 2 cut(s) 44, 151
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 6 cut(s) 480, 487, 671, 1340, 1373, 2762
ScrFI CCNGG 7 cut(s) 23, 557, 1257, 1360, 1911, 2524, 2679
SfaNI GCATC 8 cut(s) 199, 1810, 1939, 2190, 2302, 2380, 2838, 2885
SfcI CTRYAG 2 cut(s) 453, 462
SfoI GGCGCC 1 cut(s) 64
Sfr303I CCGCGG 2 cut(s) 58, 192
SfuI TTCGAA 1 cut(s) 445
SgrBI CCGCGG 2 cut(s) 58, 192
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 342, 2846
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
Sse9I AATT 9 cut(s) 280, 1140, 1354, 1605, 1725, 1977, 2247, 2577, 2906
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 1 cut(s) 1695
SspMI CTAG 7 cut(s) 329, 839, 1289, 1469, 1733, 2261, 2970
StyD4I CCNGG 7 cut(s) 21, 555, 1255, 1358, 1909, 2522, 2677
StyI CCWWGG 1 cut(s) 1624
TaaI ACNGT 5 cut(s) 577, 625, 967, 1068, 1519
TaiI ACGT 7 cut(s) 280, 531, 713, 859, 1168, 1268, 1299
TaqI TCGA 7 cut(s) 445, 982, 1162, 2100, 2269, 2498, 2667
TasI AATT 9 cut(s) 280, 1140, 1354, 1605, 1725, 1977, 2247, 2577, 2906
TatI WGTACW 3 cut(s) 219, 2168, 3025
TfiI GAWTC 9 cut(s) 442, 952, 979, 989, 1620, 1942, 2075, 2339, 2899
TscAI CASTG 3 cut(s) 543, 2353, 2618
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 6 cut(s) 33, 923, 2026, 2252, 2255, 2315
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 9 cut(s) 327, 339, 410, 692, 1113, 1122, 1133, 1633, 2855
TspRI CASTG 3 cut(s) 543, 2353, 2618
Van91I CCANNNNNTGG 3 cut(s) 1090, 2192, 2560
Vha464I CTTAAG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 151
XagI CCTNNNNNAGG 1 cut(s) 940
XapI RAATTY 2 cut(s) 1354, 1977
XbaI TCTAGA 3 cut(s) 838, 1288, 1468
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 723
XspI CTAG 7 cut(s) 329, 839, 1289, 1469, 1733, 2261, 2970
ZraI GACGTC 1 cut(s) 1166
ZrmI AGTACT 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.