Rh3AG204500

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007834
Physical Location & Seq
Reverse (-)
20300046 .. 20308664
8619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG204500.1

Sequence Viewer

Length: 3036 bp
ATGTCCAGCCGCTCCAACTTCCAGGGCGGCCGCAGCAGAGGCGGCCCTAACTCCCGCGGCGGCGCTCGCCGTGGAGGCGGCGCTCGCGGCGGTGGAGGCCGAGGAGGAGGACGCGGCGGCGGCGGCGGCCGAGGCGGCGAACAGCGCTGGTGGGACCCCGTGTGGCGAGCCGAGCGACTGAGGCAACAAGCCGCCGAGATGGAAGTTCTTGATGCGAGTGAGTATTGGGGGAAGATGGAGCAGTTCAGAAGCGGAGCGGAGCAGGAGATGATAATAAGACGTAATTTTAGTCGGCATGATCAGCAAACCCTATATGAAATGGCCGATGAACTAGGGCTTCACTTCCATGCATATAATAAGGGGAAGGCTCTTGTGGTCAGCAAGGTTCCTCTGCCAGATTATCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGGATGTCTACAGAGACTGCAGAGAGAGTCGGGAGTCTTTTGCAAAGCTCGCAGGGGCAGGGAAGTAAGCAAGCTTTTGGTAATGTTGCTTCAGTGGCGTCTGGGTCTGGCCAGGGGAACAAACTAACAACTATTAGTGAAAATACAACCAAACCCAGCTCCAGCTTGGAGCTTGATACTGTTAAGGAGAAAGAGAAACTTAGCCTTCAACTTAAGGAGTCGCAGGAAAAAATGAAGGTAAGTAATAGTCTGAAAGCAATGCTGACATTCCGAGAAAAGCTTCCTGCATTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGAGTGCAACATAATATGCACTCAGCCTCGCCGTATATCTGCCGTGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACAACTGGAGTATTACTTCGTCAGTTGGTTCAAGACCCACAGTTAACTGGCGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAACGAGGACTTTTTACTAATAATTTTGCGAGACCTTCTTCCTCGACGTCCAGATCTACGACTTATTCTAATGAGTGCTACCATCAATGCTGACTTGTTTTCCAAATACTTTGGAAATGCCCCAACAATACATATACCGGGATTAACGTTCCCTGTCGCAGAGCTATTTCTAGAAGACGTTTTGGAGAAAACTCATTATATAGTCAAGTCAGAGTCCGACAAGATGGAGAGGGGAAATTCCAGGAGAAGGAGACAGCAAGACTCCAAGAAGGATCCCTTAACTGAACTATTTGAGGATGTTGATATTGATCTTCACTACAAAAGTTACAGCACATCTACAAGAAAGTCTCTAGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTCTGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACAGGCTGGGATGATATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCTGAGAAGTTCATGGTTCTTCCCCTTCATGGGTCAATGCCTACTATCAATCAACGAGAAATATTTGATCGTCCTCCCACTACCAAGAGAAAAATTGTGCTAGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGTGGAAAGGCAAAGGAAACGAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATACCCAAAAATGATTCACGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTTTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCCTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGAGCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTCAACAAATCCAGGGGTGCTAACGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTTGGTAAAGTCGATATCCATCCTGGATCAGTCAATGCTGGGGTTCACTTATTCCCTCTGCCTTACATGGTATATAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTTGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTAGTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1011

Amino Acids

112.25

Weight (kDa)

8.72

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 250 - 404 2.9e-09 DEAD/DEAH box helicase
Helicase_C PF00271 509 - 636 2e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 700 - 728 1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 729 - 788 2.4e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 859 - 939 9.8e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 949 - 1006 1.3e-21 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1171
AccB7I CCANNNNNTGG 3 cut(s) 1093, 2195, 2563
AccBSI CCGCTC 2 cut(s) 12, 257
AccI GTMKAC 1 cut(s) 455
AccII CGCG 3 cut(s) 57, 87, 114
AccIII TCCGGA 1 cut(s) 1986
AclI AACGTT 1 cut(s) 1268
AclWI GGATC 8 cut(s) 1388, 1401, 1628, 2053, 2180, 2193, 2416, 2692
AcoI YGGCCR 5 cut(s) 28, 127, 321, 556, 1900
AcsI RAATTY 2 cut(s) 1357, 1980
AcuI CTGAAG 2 cut(s) 522, 1857
AcyI GRCGYC 2 cut(s) 545, 1168
AfaI GTAC 2 cut(s) 2173, 3030
AfeI AGCGCT 2 cut(s) 146, 1001
AfiI CCNNNNNNNGG 8 cut(s) 945, 1093, 1368, 1664, 1665, 2195, 2563, 2962
AflII CTTAAG 1 cut(s) 659
AgsI TTSAA 8 cut(s) 656, 739, 1061, 1866, 2069, 2093, 2228, 2381
AjiI CACGTC 1 cut(s) 860
AjnI CCWGG 6 cut(s) 21, 558, 1361, 1912, 2525, 2680
AlwI GGATC 8 cut(s) 1388, 1401, 1628, 2053, 2180, 2193, 2416, 2692
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 793
Aor13HI TCCGGA 1 cut(s) 1986
Aor51HI AGCGCT 2 cut(s) 146, 1001
AoxI GGCC 7 cut(s) 28, 43, 97, 127, 321, 556, 1900
ApeKI GCWGC 6 cut(s) 33, 926, 2029, 2255, 2258, 2318
ApoI RAATTY 2 cut(s) 1357, 1980
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 5 cut(s) 65, 83, 147, 1002, 2916
AspS9I GGNCC 2 cut(s) 44, 154
AsuC2I CCSGG 1 cut(s) 1260
AsuHPI GGTGA 4 cut(s) 809, 974, 2351, 2436
AsuNHI GCTAGC 1 cut(s) 1735
AvaI CYCGRG 1 cut(s) 793
AvaII GGWCC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 558
BamHI GGATCC 2 cut(s) 1393, 2185
BarI GAAGNNNNNNTAC 2 cut(s) 520, 552
BbsI GAAGAC 2 cut(s) 1302, 2753
BbvI GCAGC 6 cut(s) 45, 913, 2041, 2242, 2245, 2305
BceAI ACGGC 5 cut(s) 54, 888, 899, 1887, 2901
BciT130I CCWGG 6 cut(s) 23, 560, 1363, 1914, 2527, 2682
BclI TGATCA 2 cut(s) 298, 2353
BcnI CCSGG 1 cut(s) 1260
BfaI CTAG 7 cut(s) 332, 842, 1292, 1472, 1736, 2264, 2973
BfmI CTRYAG 2 cut(s) 456, 465
BfoI RGCGCY 4 cut(s) 66, 84, 148, 1003
BfrI CTTAAG 1 cut(s) 659
BglI GCCNNNNNGGC 2 cut(s) 75, 135
BglII AGATCT 2 cut(s) 1174, 2491
Bme1390I CCNGG 7 cut(s) 23, 560, 1260, 1363, 1914, 2527, 2682
Bme18I GGWCC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 793
BmgBI CACGTC 1 cut(s) 860
BmgT120I GGNCC 2 cut(s) 44, 154
BmiI GGNNCC 6 cut(s) 155, 156, 387, 430, 1395, 2187
BmrFI CCNGG 7 cut(s) 23, 560, 1260, 1363, 1914, 2527, 2682
BmsI GCATC 8 cut(s) 202, 1813, 1942, 2193, 2305, 2383, 2841, 2888
BmtI GCTAGC 1 cut(s) 1739
BpiI GAAGAC 2 cut(s) 1302, 2753
BpmI CTGGAG 4 cut(s) 592, 1056, 1935, 2856
BpuMI CCSGG 1 cut(s) 1260
BsaBI GATNNNNATC 2 cut(s) 1428, 2676
BsaHI GRCGYC 2 cut(s) 545, 1168
BsaI GGTCTC 2 cut(s) 1146, 2268
BsaJI CCNNGG 8 cut(s) 22, 55, 70, 100, 130, 559, 1627, 2526
BsaWI WCCGGW 1 cut(s) 1986
BsaXI ACNNNNNCTCC 2 cut(s) 2558, 2588
Bsc4I CCNNNNNNNGG 8 cut(s) 945, 1093, 1368, 1664, 1665, 2195, 2563, 2962
Bse1I ACTGG 5 cut(s) 993, 1039, 1081, 2447, 2839
Bse3DI GCAATG 3 cut(s) 711, 1746, 1962
Bse8I GATNNNNATC 2 cut(s) 1428, 2676
BseAI TCCGGA 1 cut(s) 1986
BseBI CCWGG 6 cut(s) 23, 560, 1363, 1914, 2527, 2682
BseDI CCNNGG 8 cut(s) 22, 55, 70, 100, 130, 559, 1627, 2526
BseGI GGATG 6 cut(s) 456, 1423, 1588, 2390, 2677, 2971
BseJI GATNNNNATC 2 cut(s) 1428, 2676
BseLI CCNNNNNNNGG 8 cut(s) 945, 1093, 1368, 1664, 1665, 2195, 2563, 2962
BseMI GCAATG 3 cut(s) 711, 1746, 1962
BseMII CTCAG 7 cut(s) 170, 741, 749, 845, 864, 908, 1629
BseNI ACTGG 5 cut(s) 993, 1039, 1081, 2447, 2839
BseRI GAGGAG 3 cut(s) 117, 120, 2156
BseX3I CGGCCG 2 cut(s) 28, 127
BseXI GCAGC 6 cut(s) 45, 913, 2041, 2242, 2245, 2305
BseYI CCCAGC 5 cut(s) 602, 1578, 2591, 2696, 2956
BsgI GTGCAG 1 cut(s) 2633
Bsh1236I CGCG 3 cut(s) 57, 87, 114
Bsh1285I CGRYCG 2 cut(s) 31, 130
BshFI GGCC 7 cut(s) 30, 45, 99, 129, 323, 558, 1902
BsiEI CGRYCG 2 cut(s) 31, 130
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 2 cut(s) 1259, 1987
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 8 cut(s) 945, 1093, 1368, 1664, 1665, 2195, 2563, 2962
BsmBI CGTCTC 1 cut(s) 1879
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 734, 2642
BsnI GGCC 7 cut(s) 30, 45, 99, 129, 323, 558, 1902
Bso31I GGTCTC 2 cut(s) 1146, 2268
BsoBI CYCGRG 1 cut(s) 793
Bsp13I TCCGGA 1 cut(s) 1986
Bsp1407I TGTACA 2 cut(s) 2171, 3028
BspANI GGCC 7 cut(s) 30, 45, 99, 129, 323, 558, 1902
BspCNI CTCAG 7 cut(s) 171, 742, 750, 844, 865, 907, 1630
BspEI TCCGGA 1 cut(s) 1986
BspFNI CGCG 3 cut(s) 57, 87, 114
BspLI GGNNCC 6 cut(s) 155, 156, 387, 430, 1395, 2187
BspMAI CTGCAG 1 cut(s) 469
BspOI GCTAGC 1 cut(s) 1739
BspPI GGATC 8 cut(s) 1388, 1401, 1628, 2053, 2180, 2193, 2416, 2692
BspTI CTTAAG 1 cut(s) 659
BspTNI GGTCTC 2 cut(s) 1146, 2268
BsrBI CCGCTC 2 cut(s) 12, 257
BsrDI GCAATG 3 cut(s) 711, 1746, 1962
BsrGI TGTACA 2 cut(s) 2171, 3028
BsrI ACTGG 5 cut(s) 993, 1039, 1081, 2447, 2839
BssECI CCNNGG 8 cut(s) 22, 55, 70, 100, 130, 559, 1627, 2526
BssNI GRCGYC 2 cut(s) 545, 1168
BssT1I CCWWGG 1 cut(s) 1627
Bst2UI CCWGG 6 cut(s) 23, 560, 1363, 1914, 2527, 2682
Bst4CI ACNGT 5 cut(s) 628, 970, 1071, 1522, 1796
Bst6I CTCTTC 1 cut(s) 2620
BstACI GRCGYC 2 cut(s) 545, 1168
BstAFI CTTAAG 1 cut(s) 659
BstAUI TGTACA 2 cut(s) 2171, 3028
BstC8I GCNNGC 9 cut(s) 67, 85, 168, 497, 519, 1737, 1896, 1900, 2755
BstDEI CTNAG 8 cut(s) 179, 647, 750, 758, 831, 873, 894, 1638
BstDSI CCRYGG 2 cut(s) 55, 70
BstEII GGTNACC 1 cut(s) 2861
BstENI CCTNNNNNAGG 1 cut(s) 943
BstF5I GGATG 6 cut(s) 456, 1423, 1588, 2390, 2677, 2971
BstFNI CGCG 3 cut(s) 57, 87, 114
BstH2I RGCGCY 4 cut(s) 66, 84, 148, 1003
BstHHI GCGC 5 cut(s) 65, 83, 147, 1002, 2916
BstMCI CGRYCG 2 cut(s) 31, 130
BstNI CCWGG 6 cut(s) 23, 560, 1363, 1914, 2527, 2682
BstPI GGTNACC 1 cut(s) 2861
BstSCI CCNGG 7 cut(s) 21, 558, 1258, 1361, 1912, 2525, 2680
BstSFI CTRYAG 2 cut(s) 456, 465
BstUI CGCG 3 cut(s) 57, 87, 114
BstV1I GCAGC 6 cut(s) 45, 913, 2041, 2242, 2245, 2305
BstV2I GAAGAC 2 cut(s) 1302, 2753
BstX2I RGATCY 5 cut(s) 1174, 1393, 1633, 2185, 2491
BstYI RGATCY 5 cut(s) 1174, 1393, 1633, 2185, 2491
BstZI CGGCCG 2 cut(s) 28, 127
BsuRI GGCC 7 cut(s) 30, 45, 99, 129, 323, 558, 1902
BtgI CCRYGG 2 cut(s) 55, 70
BtrI CACGTC 1 cut(s) 860
BtsCI GGATG 6 cut(s) 456, 1423, 1588, 2390, 2677, 2971
BtsI GCAGTG 1 cut(s) 2621
BtsIMutI CAGTG 3 cut(s) 546, 2356, 2621
Cac8I GCNNGC 9 cut(s) 67, 85, 168, 497, 519, 1737, 1896, 1900, 2755
CaiI CAGNNNCTG 1 cut(s) 464
CciNI GCGGCCGC 1 cut(s) 28
CfoI GCGC 5 cut(s) 65, 83, 147, 1002, 2916
Cfr13I GGNCC 2 cut(s) 44, 154
Cfr42I CCGCGG 1 cut(s) 58
CseI GACGC 2 cut(s) 120, 534
Csp6I GTAC 2 cut(s) 2172, 3029
CviQI GTAC 2 cut(s) 2172, 3029
DdeI CTNAG 8 cut(s) 179, 647, 750, 758, 831, 873, 894, 1638
DraI TTTAAA 2 cut(s) 1830, 2937
EaeI YGGCCR 5 cut(s) 28, 127, 321, 556, 1900
EagI CGGCCG 2 cut(s) 28, 127
Eam1104I CTCTTC 1 cut(s) 2620
EarI CTCTTC 1 cut(s) 2620
EclXI CGGCCG 2 cut(s) 28, 127
Eco130I CCWWGG 1 cut(s) 1627
Eco31I GGTCTC 2 cut(s) 1146, 2268
Eco32I GATATC 1 cut(s) 2674
Eco47I GGWCC 1 cut(s) 154
Eco47III AGCGCT 2 cut(s) 146, 1001
Eco52I CGGCCG 2 cut(s) 28, 127
Eco57I CTGAAG 2 cut(s) 522, 1857
Eco88I CYCGRG 1 cut(s) 793
Eco91I GGTNACC 1 cut(s) 2861
EcoNI CCTNNNNNAGG 1 cut(s) 943
EcoO109I RGGNCCY 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 2861
EcoRII CCWGG 6 cut(s) 21, 558, 1361, 1912, 2525, 2680
EcoRV GATATC 1 cut(s) 2674
EcoT14I CCWWGG 1 cut(s) 1627
EcoT22I ATGCAT 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 1627
Esp3I CGTCTC 1 cut(s) 1879
FalI AAGNNNNNCTT 4 cut(s) 630, 662, 1382, 1414
FaqI GGGAC 1 cut(s) 167
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 2 cut(s) 298, 2353
FblI GTMKAC 1 cut(s) 455
FokI GGATG 6 cut(s) 463, 1430, 1595, 2397, 2664, 2978
FspBI CTAG 7 cut(s) 332, 842, 1292, 1472, 1736, 2264, 2973
GlaI GCGC 5 cut(s) 64, 82, 146, 1001, 2915
GsaI CCCAGC 5 cut(s) 606, 1582, 2595, 2700, 2960
GsuI CTGGAG 4 cut(s) 592, 1056, 1935, 2856
HaeII RGCGCY 4 cut(s) 66, 84, 148, 1003
HaeIII GGCC 7 cut(s) 30, 45, 99, 129, 323, 558, 1902
HapII CCGG 2 cut(s) 1259, 1987
HgaI GACGC 2 cut(s) 120, 534
HhaI GCGC 5 cut(s) 65, 83, 147, 1002, 2916
Hin1I GRCGYC 2 cut(s) 545, 1168
Hin6I GCGC 5 cut(s) 63, 81, 145, 1000, 2914
HinP1I GCGC 5 cut(s) 63, 81, 145, 1000, 2914
HincII GTYRAC 2 cut(s) 1074, 2518
HindII GTYRAC 2 cut(s) 1074, 2518
HindIII AAGCTT 5 cut(s) 519, 725, 1476, 2373, 2930
HpaI GTTAAC 1 cut(s) 1074
HpaII CCGG 2 cut(s) 1259, 1987
HphI GGTGA 4 cut(s) 809, 974, 2351, 2436
Hpy166II GTNNAC 8 cut(s) 456, 1074, 1490, 2174, 2518, 2704, 2921, 3031
Hpy8I GTNNAC 8 cut(s) 456, 1074, 1490, 2174, 2518, 2704, 2921, 3031
Hpy99I CGWCG 1 cut(s) 1170
HpyAV CCTTC 9 cut(s) 358, 662, 676, 1166, 1362, 1384, 1670, 2375, 2876
HpyCH4III ACNGT 5 cut(s) 628, 970, 1071, 1522, 1796
HpyCH4IV ACGT 5 cut(s) 280, 859, 1168, 1268, 1299
HpyF3I CTNAG 8 cut(s) 179, 647, 750, 758, 831, 873, 894, 1638
HpySE526I ACGT 5 cut(s) 280, 859, 1168, 1268, 1299
Hsp92I GRCGYC 2 cut(s) 545, 1168
HspAI GCGC 5 cut(s) 63, 81, 145, 1000, 2914
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 1 cut(s) 1986
Ksp22I TGATCA 2 cut(s) 298, 2353
KspAI GTTAAC 1 cut(s) 1074
KspI CCGCGG 1 cut(s) 58
Lsp1109I GCAGC 6 cut(s) 45, 913, 2041, 2242, 2245, 2305
LweI GCATC 8 cut(s) 202, 1813, 1942, 2193, 2305, 2383, 2841, 2888
MaeI CTAG 7 cut(s) 332, 842, 1292, 1472, 1736, 2264, 2973
MaeII ACGT 5 cut(s) 280, 859, 1168, 1268, 1299
MaeIII GTNAC 6 cut(s) 1445, 1596, 1922, 1971, 2449, 2861
MbiI CCGCTC 2 cut(s) 12, 257
MboII GAAGA 9 cut(s) 244, 857, 1151, 1307, 1424, 1646, 2637, 2758, 2956
MfeI CAATTG 1 cut(s) 2250
MflI RGATCY 5 cut(s) 1174, 1393, 1633, 2185, 2491
MlsI TGGCCA 1 cut(s) 558
MluCI AATT 9 cut(s) 283, 1143, 1357, 1608, 1728, 1980, 2250, 2580, 2909
MluNI TGGCCA 1 cut(s) 558
MlyI GAGTC 6 cut(s) 483, 490, 674, 1343, 1376, 2765
MmeI TCCRAC 5 cut(s) 39, 1362, 1933, 2023, 2849
Mox20I TGGCCA 1 cut(s) 558
Mph1103I ATGCAT 1 cut(s) 352
MroI TCCGGA 1 cut(s) 1986
MroXI GAANNNNTTC 1 cut(s) 726
MscI TGGCCA 1 cut(s) 558
MslI CAYNNNNRTG 2 cut(s) 345, 2849
Msp20I TGGCCA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 57
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 2 cut(s) 1259, 1987
MspR9I CCNGG 7 cut(s) 23, 560, 1260, 1363, 1914, 2527, 2682
MunI CAATTG 1 cut(s) 2250
Mva1269I GAATGC 2 cut(s) 734, 2642
MvaI CCWGG 6 cut(s) 23, 560, 1363, 1914, 2527, 2682
MvnI CGCG 3 cut(s) 57, 87, 114
NciI CCSGG 1 cut(s) 1260
NheI GCTAGC 1 cut(s) 1735
NlaIV GGNNCC 6 cut(s) 155, 156, 387, 430, 1395, 2187
NmeAIII GCCGAG 4 cut(s) 125, 155, 196, 220
NotI GCGGCCGC 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 352
PctI GAATGC 2 cut(s) 734, 2642
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 9 cut(s) 445, 955, 982, 992, 1623, 1945, 2078, 2342, 2902
PflMI CCANNNNNTGG 3 cut(s) 1093, 2195, 2563
PfoI TCCNGGA 2 cut(s) 1361, 2680
PleI GAGTC 6 cut(s) 482, 489, 673, 1342, 1376, 2765
PpsI GAGTC 6 cut(s) 482, 489, 673, 1342, 1376, 2765
PpuMI RGGWCCY 1 cut(s) 154
Psp1406I AACGTT 1 cut(s) 1268
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 6 cut(s) 21, 558, 1361, 1912, 2525, 2680
PspEI GGTNACC 1 cut(s) 2861
PspFI CCCAGC 5 cut(s) 602, 1578, 2591, 2696, 2956
PspGI CCWGG 6 cut(s) 21, 558, 1361, 1912, 2525, 2680
PspN4I GGNNCC 6 cut(s) 155, 156, 387, 430, 1395, 2187
PspPI GGNCC 2 cut(s) 44, 154
PspPPI RGGWCCY 1 cut(s) 154
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 5 cut(s) 1174, 1393, 1633, 2185, 2491
RsaI GTAC 2 cut(s) 2173, 3030
RsaNI GTAC 2 cut(s) 2172, 3029
RseI CAYNNNNRTG 2 cut(s) 345, 2849
SacII CCGCGG 1 cut(s) 58
Sau96I GGNCC 2 cut(s) 44, 154
SchI GAGTC 6 cut(s) 483, 490, 674, 1343, 1376, 2765
ScrFI CCNGG 7 cut(s) 23, 560, 1260, 1363, 1914, 2527, 2682
SfaNI GCATC 8 cut(s) 202, 1813, 1942, 2193, 2305, 2383, 2841, 2888
SfcI CTRYAG 2 cut(s) 456, 465
Sfr303I CCGCGG 1 cut(s) 58
SgrBI CCGCGG 1 cut(s) 58
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 2 cut(s) 345, 2849
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
Sse9I AATT 9 cut(s) 283, 1143, 1357, 1608, 1728, 1980, 2250, 2580, 2909
SspI AATATT 1 cut(s) 1698
SspMI CTAG 7 cut(s) 332, 842, 1292, 1472, 1736, 2264, 2973
StyD4I CCNGG 7 cut(s) 21, 558, 1258, 1361, 1912, 2525, 2680
StyI CCWWGG 1 cut(s) 1627
TaaI ACNGT 5 cut(s) 628, 970, 1071, 1522, 1796
TaiI ACGT 5 cut(s) 283, 862, 1171, 1271, 1302
TaqI TCGA 6 cut(s) 985, 1165, 2103, 2272, 2501, 2670
TasI AATT 9 cut(s) 283, 1143, 1357, 1608, 1728, 1980, 2250, 2580, 2909
TatI WGTACW 2 cut(s) 2171, 3028
TfiI GAWTC 9 cut(s) 445, 955, 982, 992, 1623, 1945, 2078, 2342, 2902
TscAI CASTG 3 cut(s) 546, 2356, 2621
TseI GCWGC 6 cut(s) 33, 926, 2029, 2255, 2258, 2318
TspRI CASTG 3 cut(s) 546, 2356, 2621
Van91I CCANNNNNTGG 3 cut(s) 1093, 2195, 2563
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 154
XagI CCTNNNNNAGG 1 cut(s) 943
XapI RAATTY 2 cut(s) 1357, 1980
XbaI TCTAGA 3 cut(s) 841, 1291, 1471
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmiI GTMKAC 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 7 cut(s) 332, 842, 1292, 1472, 1736, 2264, 2973
ZraI GACGTC 1 cut(s) 1169
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.