RLG00000023783

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007834
Physical Location & Seq
Forward (+)
28132852 .. 28134755
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023783

Sequence Viewer

Length: 1014 bp
ATGATTCACGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCCTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCGCCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTAGAGCTTTAGATGATATGGAGGAGCTTACTCTGCTTGGTATGTCGCCATCTTTGCACACTACCATTGCAAACATTGGAAAGTTGCTTCTAATGGGTTCTATTTTTCAATGCCTTAATCCTGCGTTAACAATTGCTGCTGCCCTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGATCCTTCGCTGGTGATTCTTTTAGTGATCACATAGAAGTTGTTAAAGCTTTTGAAGGATGGAAAGATACAAAACGTAATGGATCAGAAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGGTGCAGTTTGCTTACAATCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTTGGTAAAGTTGATATCCATCCTGGATCAGTCAATGCTGGCGTTCATTTATTCTCTCTGCCCTACATGGTATACAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGAGGGCATTGAGATGCTTGGAGGCTACCTTCATTTCTCTGCATCAATGAGTATTTTGGAGTTGATTCGGAGATTGCGCCGTGAACTTGACAGGCTTTTAAACAGGAAGATTGACAAGCCAGCATTGGACGTCTCAAGTGAGGGAAAGGGGCTGGTATCTGCTGTGGTTGAGCTATTGCATAGCTCAAATGTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

338

Amino Acids

37.64

Weight (kDa)

6.37

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 85 - 140 2.3e-11 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 195 - 272 1.1e-16 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 275 - 332 1.1e-20 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 948
AccB7I CCANNNNNTGG 1 cut(s) 571
AccI GTMKAC 1 cut(s) 738
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 2 cut(s) 131, 643
AclWI GGATC 4 cut(s) 112, 381, 475, 700
AcsI RAATTY 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 945
AfiI CCNNNNNNNGG 1 cut(s) 571
AgsI TTSAA 4 cut(s) 128, 152, 287, 440
AjnI CCWGG 2 cut(s) 91, 688
AluBI AGCT 8 cut(s) 98, 185, 205, 326, 371, 434, 988, 999
AluI AGCT 8 cut(s) 98, 185, 205, 326, 371, 434, 988, 999
Alw26I GTCTC 3 cut(s) 327, 771, 952
AlwI GGATC 4 cut(s) 112, 381, 475, 700
Aor13HI TCCGGA 1 cut(s) 45
ApeKI GCWGC 4 cut(s) 88, 314, 317, 377
ApoI RAATTY 1 cut(s) 39
AspLEI GCGC 1 cut(s) 894
AsuHPI GGTGA 2 cut(s) 410, 495
BbsI GAAGAC 1 cut(s) 761
BbvI GCAGC 4 cut(s) 100, 301, 304, 364
BccI CCATC 6 cut(s) 167, 235, 438, 517, 571, 693
BceAI ACGGC 1 cut(s) 879
BciT130I CCWGG 2 cut(s) 93, 690
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 3 cut(s) 327, 771, 952
BfaI CTAG 1 cut(s) 323
BisI GCNGC 4 cut(s) 89, 315, 318, 378
BlsI GCNGC 4 cut(s) 90, 316, 319, 379
Bme1390I CCNGG 2 cut(s) 93, 690
BmrFI CCNGG 2 cut(s) 93, 690
BmsI GCATC 3 cut(s) 364, 819, 866
BpiI GAAGAC 1 cut(s) 761
BpmI CTGGAG 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 934
BsaBI GATNNNNATC 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 327
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 1 cut(s) 571
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 2 cut(s) 21, 243
Bse8I GATNNNNATC 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 2 cut(s) 93, 690
BseGI GGATG 2 cut(s) 449, 685
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 1 cut(s) 571
BseMI GCAATG 2 cut(s) 21, 243
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 4 cut(s) 100, 301, 304, 364
BseYI CCCAGC 1 cut(s) 599
BsgI GTGCAG 2 cut(s) 560, 641
BsiSI CCGG 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 571
BsmAI GTCTC 3 cut(s) 327, 771, 952
BsmBI CGTCTC 1 cut(s) 952
BsmI GAATGC 1 cut(s) 650
Bso31I GGTCTC 1 cut(s) 327
Bsp13I TCCGGA 1 cut(s) 45
Bsp143I GATC 5 cut(s) 104, 386, 412, 467, 692
BspACI CCGC 2 cut(s) 131, 643
BspEI TCCGGA 1 cut(s) 45
BspPI GGATC 4 cut(s) 112, 381, 475, 700
BspTNI GGTCTC 1 cut(s) 327
BsrDI GCAATG 2 cut(s) 21, 243
BsrI ACTGG 1 cut(s) 506
BssMI GATC 5 cut(s) 104, 386, 412, 467, 692
BssNAI GTATAC 1 cut(s) 739
BssNI GRCGYC 1 cut(s) 945
Bst1107I GTATAC 1 cut(s) 739
Bst2UI CCWGG 2 cut(s) 93, 690
Bst4CI ACNGT 1 cut(s) 743
Bst6I CTCTTC 1 cut(s) 628
BstACI GRCGYC 1 cut(s) 945
BstC8I GCNNGC 3 cut(s) 706, 763, 936
BstF5I GGATG 2 cut(s) 449, 685
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 5 cut(s) 107, 389, 415, 470, 695
BstMAI GTCTC 3 cut(s) 327, 771, 952
BstMBI GATC 5 cut(s) 104, 386, 412, 467, 692
BstMWI GCNNNNNNNGC 4 cut(s) 211, 232, 323, 377
BstNI CCWGG 2 cut(s) 93, 690
BstSCI CCNGG 2 cut(s) 91, 688
BstV1I GCAGC 4 cut(s) 100, 301, 304, 364
BstV2I GAAGAC 1 cut(s) 761
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BstZ17I GTATAC 1 cut(s) 739
BtsCI GGATG 2 cut(s) 449, 685
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 2 cut(s) 629, 748
Cac8I GCNNGC 3 cut(s) 706, 763, 936
CfoI GCGC 1 cut(s) 894
CviAII CATG 2 cut(s) 566, 733
DpnI GATC 5 cut(s) 106, 388, 414, 469, 694
DpnII GATC 5 cut(s) 104, 386, 412, 467, 692
DraI TTTAAA 1 cut(s) 915
Eam1104I CTCTTC 1 cut(s) 628
EarI CTCTTC 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 91, 688
EcoRV GATATC 1 cut(s) 682
Esp3I CGTCTC 1 cut(s) 952
FaeI CATG 2 cut(s) 569, 736
FatI CATG 2 cut(s) 565, 732
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 738
Fnu4HI GCNGC 4 cut(s) 89, 315, 318, 378
FokI GGATG 2 cut(s) 456, 672
Fsp4HI GCNGC 4 cut(s) 89, 315, 318, 378
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 893
GluI GCNGC 4 cut(s) 89, 315, 318, 378
GsaI CCCAGC 1 cut(s) 603
GsuI CTGGAG 1 cut(s) 114
HapII CCGG 1 cut(s) 46
HhaI GCGC 1 cut(s) 894
Hin1I GRCGYC 1 cut(s) 945
Hin1II CATG 2 cut(s) 569, 736
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 5 cut(s) 4, 137, 401, 779, 880
HpaI GTTAAC 1 cut(s) 306
HpaII CCGG 1 cut(s) 46
HphI GGTGA 2 cut(s) 410, 495
Hpy166II GTNNAC 4 cut(s) 306, 739, 899, 1009
Hpy188I TCNGA 4 cut(s) 472, 776, 796, 885
Hpy188III TCNNGA 3 cut(s) 8, 46, 332
Hpy8I GTNNAC 4 cut(s) 306, 739, 899, 1009
HpyAV CCTTC 3 cut(s) 400, 434, 854
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4IV ACGT 2 cut(s) 460, 945
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 232, 323, 377
HpySE526I ACGT 2 cut(s) 460, 945
Hsp92I GRCGYC 1 cut(s) 945
Hsp92II CATG 2 cut(s) 569, 736
HspAI GCGC 1 cut(s) 892
Kpn2I TCCGGA 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 412
KspAI GTTAAC 1 cut(s) 306
Kzo9I GATC 5 cut(s) 104, 386, 412, 467, 692
LmnI GCTCC 3 cut(s) 24, 95, 202
Lsp1109I GCAGC 4 cut(s) 100, 301, 304, 364
LweI GCATC 3 cut(s) 364, 819, 866
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 460, 945
MaeIII GTNAC 2 cut(s) 30, 508
MalI GATC 5 cut(s) 106, 388, 414, 469, 694
MboI GATC 5 cut(s) 104, 386, 412, 467, 692
MboII GAAGA 3 cut(s) 645, 766, 934
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 386
MluCI AATT 3 cut(s) 39, 309, 588
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 9 cut(s) 80, 193, 358, 520, 528, 629, 811, 830, 949
MroI TCCGGA 1 cut(s) 45
MseI TTAA 4 cut(s) 294, 305, 429, 914
MslI CAYNNNNRTG 1 cut(s) 827
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 2 cut(s) 93, 690
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 2 cut(s) 93, 690
MwoI GCNNNNNNNGC 4 cut(s) 211, 232, 323, 377
NdeII GATC 5 cut(s) 104, 386, 412, 467, 692
NlaIII CATG 2 cut(s) 569, 736
PctI GAATGC 1 cut(s) 650
PfeI GAWTC 4 cut(s) 4, 137, 401, 880
PflMI CCANNNNNTGG 1 cut(s) 571
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 4 cut(s) 90, 316, 319, 379
PleI GAGTC 1 cut(s) 773
PpsI GAGTC 1 cut(s) 773
Psp6I CCWGG 2 cut(s) 91, 688
PspFI CCCAGC 1 cut(s) 599
PspGI CCWGG 2 cut(s) 91, 688
PsuI RGATCY 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 827
SaqAI TTAA 4 cut(s) 294, 305, 429, 914
SatI GCNGC 4 cut(s) 89, 315, 318, 378
Sau3AI GATC 5 cut(s) 104, 386, 412, 467, 692
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 2 cut(s) 93, 690
SfaNI GCATC 3 cut(s) 364, 819, 866
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 1 cut(s) 949
SmoI CTYRAG 1 cut(s) 949
Sse9I AATT 3 cut(s) 39, 309, 588
SsiI CCGC 2 cut(s) 131, 643
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 91, 688
TaaI ACNGT 1 cut(s) 743
TaiI ACGT 2 cut(s) 463, 948
TaqI TCGA 2 cut(s) 162, 331
TasI AATT 3 cut(s) 39, 309, 588
TfiI GAWTC 4 cut(s) 4, 137, 401, 880
Tru1I TTAA 4 cut(s) 294, 305, 429, 914
Tru9I TTAA 4 cut(s) 294, 305, 429, 914
TscAI CASTG 2 cut(s) 629, 748
TseI GCWGC 4 cut(s) 88, 314, 317, 377
TspDTI ATGAA 2 cut(s) 701, 836
TspRI CASTG 2 cut(s) 629, 748
Van91I CCANNNNNTGG 1 cut(s) 571
XapI RAATTY 1 cut(s) 39
XmiI GTMKAC 1 cut(s) 738
XspI CTAG 1 cut(s) 323
ZraI GACGTC 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.