Rh3BG235900

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0007834
Physical Location & Seq
Reverse (-)
22066452 .. 22075323
8872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG235900.1

Sequence Viewer

Length: 3015 bp
ATGTCCAGCCGCTCCAACTTCCAGGGCGGCCGCAGCAGAGGCGGCCCTAACTCCCGCGGCGGCGCTCGCCGTGGAGGCCGAGGAGGAGGACGCGGCGGCGGCGGCGGCCGAGGCGGCGAACAGCGCTGGTGGGACCCCGTGTGGCGAGCCGAGCGACTGAGGCAACAAGCCGCGGAGATGGAAGTTCTTGATGCGAGTGAGTATTGGGGGAAGATGGAGCAGTTCAGAAGCGGAGCGGAGCAGGAGATGATAATAAGACGTAATTTTAGTCGGCATGATCAGCAAACCCTATATGAAATGGCCTATGAACTAGGGCTTCACTTCCATGCATATAATAAGGGGAAGGCTCTTGTGGTCAGCAAGGTTCCTCTGCCAGATTATCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGGATGTCTACAGAGACTGCAGAGAGAGTCGGGAGTCTTTTGCAAAGCTCGCAGGGGCAGGGAAGTAAGCAAGCTTTTGGTAATGTTGCTTCAGTGGCGTCTGGGTCTGGCCAGGGGAACAAACTAACAACTGTTAGTGAAAATACAACCAAACCCAGCTCCAGCTTGGAACCTGATACTGTTAAGGAGAAAGAGAAACTTAGCCTTCAACTTAAGGACTTGCAGGAAAAAATGAAGGTAAGTAATAGTCTGAAAGCAATGCTGACGTTCCGAGAAAAGCTTCCTGCATTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGAGTGCAACATAATATGCACTCAGCCTCGTCGTATATCTGCCATGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACAACTGGAGTATTACTTCGTCAGTTGGTTCAAGACCCACAGTTAACTGGTGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAACGAGGACTTTTTACTAATAATTTTGCGAGACCTTCTTCCTCGACGTCCAGATCTACGACTTATTCTAATGAGTGCTACCATCAATGCTGACTTGTTTTCCAAATACTTTGGAAATGCCCCAACAATACATATACCGGGATTAACGTTCCCTGTGGCAGAGCTATTTCTAGAAGACGTTTTGGAGAAAACTCATTATATAGTCGAGTCAGAGTCCGAGAAGATGGAGAGGGGAAATTCCAGGAGAAGGAGACAGCAAGACTCCAAGAAGGATCCCTTAACTGAACTATTTGAGGATGTTGATATTGATCTTCACTACAAAAGTTATAGCACATCTACAAGAAAGTCTCTGGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTCTGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACGGGCTGGGATGATATATCAAAGTTACTTGACAAAGTTAAGGGAAATAGATTCCTTGGAGATCCCGAGAAGTTCATGGTTCTTCCCCTGCATGGGTCAATGCCTTCTATCAATCAACGAGAAATATTTGATCGTCCTCCCACTAACAAAAGAAAAATTGTGCTAGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGCGGAAAGGCAAAAGAAACGAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATATCCAAAAATGATTCACGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTTTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCTTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGATCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTCGACAAATCCAGGGGTGCTAACGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGAACAACATTCTACACCAAAGAAGTTGGTAAAGTCGATATCCATCCTGGATCAGTCAATGCTGGGGTTCACTTATTCCCTCTGCCTTACATGGTATATAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGGGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTCGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTAGTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1004

Amino Acids

111.78

Weight (kDa)

8.59

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 243 - 397 1.5e-09 DEAD/DEAH box helicase
Helicase_C PF00271 502 - 629 1.1e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 693 - 721 9.9e-10 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 722 - 781 2.3e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 852 - 932 5.4e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 942 - 999 1.3e-21 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1150
AccB7I CCANNNNNTGG 3 cut(s) 1072, 2174, 2542
AccBSI CCGCTC 2 cut(s) 12, 236
AccI GTMKAC 2 cut(s) 434, 2496
AccII CGCG 3 cut(s) 57, 93, 173
AccIII TCCGGA 1 cut(s) 1965
AclI AACGTT 1 cut(s) 1247
AclWI GGATC 9 cut(s) 1367, 1380, 1607, 2032, 2159, 2172, 2301, 2395, 2671
AcoI YGGCCR 4 cut(s) 28, 106, 535, 1879
AcsI RAATTY 2 cut(s) 1336, 1959
AcuI CTGAAG 2 cut(s) 501, 1836
AcyI GRCGYC 2 cut(s) 524, 1147
AfaI GTAC 2 cut(s) 2152, 3009
AfeI AGCGCT 2 cut(s) 125, 980
AfiI CCNNNNNNNGG 8 cut(s) 924, 1072, 1347, 1643, 1644, 2174, 2542, 2941
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 8 cut(s) 635, 718, 1040, 1845, 2048, 2072, 2207, 2360
AjiI CACGTC 1 cut(s) 839
AjnI CCWGG 6 cut(s) 21, 537, 1340, 1891, 2504, 2659
AlwI GGATC 9 cut(s) 1367, 1380, 1607, 2032, 2159, 2172, 2301, 2395, 2671
AlwNI CAGNNNCTG 1 cut(s) 443
Ama87I CYCGRG 2 cut(s) 772, 1616
Aor13HI TCCGGA 1 cut(s) 1965
Aor51HI AGCGCT 2 cut(s) 125, 980
AoxI GGCC 7 cut(s) 28, 43, 76, 106, 300, 535, 1879
ApeKI GCWGC 6 cut(s) 33, 905, 2008, 2234, 2237, 2297
ApoI RAATTY 2 cut(s) 1336, 1959
Asp700I GAANNNNTTC 1 cut(s) 705
AspLEI GCGC 4 cut(s) 65, 126, 981, 2895
AspS9I GGNCC 2 cut(s) 44, 133
AsuC2I CCSGG 1 cut(s) 1239
AsuHPI GGTGA 4 cut(s) 788, 953, 2330, 2415
AsuNHI GCTAGC 1 cut(s) 1714
AvaI CYCGRG 2 cut(s) 772, 1616
AvaII GGWCC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 537
BamHI GGATCC 2 cut(s) 1372, 2164
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BbsI GAAGAC 2 cut(s) 1281, 2732
BbvI GCAGC 6 cut(s) 45, 892, 2020, 2221, 2224, 2284
BceAI ACGGC 3 cut(s) 54, 1866, 2880
BciT130I CCWGG 6 cut(s) 23, 539, 1342, 1893, 2506, 2661
BclI TGATCA 2 cut(s) 277, 2332
BcnI CCSGG 1 cut(s) 1239
BfaI CTAG 5 cut(s) 311, 821, 1271, 1715, 2952
BfmI CTRYAG 2 cut(s) 435, 444
BfoI RGCGCY 3 cut(s) 66, 127, 982
BfrI CTTAAG 1 cut(s) 638
BglI GCCNNNNNGGC 2 cut(s) 75, 114
BglII AGATCT 2 cut(s) 1153, 2470
Bme1390I CCNGG 7 cut(s) 23, 539, 1239, 1342, 1893, 2506, 2661
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 772, 1616
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 2 cut(s) 44, 133
BmiI GGNNCC 7 cut(s) 134, 135, 366, 409, 597, 1374, 2166
BmrFI CCNGG 7 cut(s) 23, 539, 1239, 1342, 1893, 2506, 2661
BmsI GCATC 8 cut(s) 181, 1792, 1921, 2172, 2284, 2362, 2820, 2867
BmtI GCTAGC 1 cut(s) 1718
BpiI GAAGAC 2 cut(s) 1281, 2732
BpmI CTGGAG 4 cut(s) 571, 1035, 1914, 2835
Bpu10I CCTNAGC 1 cut(s) 2242
BpuMI CCSGG 1 cut(s) 1239
BsaBI GATNNNNATC 2 cut(s) 1407, 2655
BsaHI GRCGYC 2 cut(s) 524, 1147
BsaI GGTCTC 2 cut(s) 1125, 2247
BsaJI CCNNGG 9 cut(s) 22, 55, 70, 79, 109, 171, 538, 1606, 2505
BsaWI WCCGGW 1 cut(s) 1965
BsaXI ACNNNNNCTCC 2 cut(s) 2537, 2567
Bsc4I CCNNNNNNNGG 8 cut(s) 924, 1072, 1347, 1643, 1644, 2174, 2542, 2941
Bse1I ACTGG 5 cut(s) 972, 1018, 1060, 2426, 2818
Bse3DI GCAATG 3 cut(s) 690, 1725, 1941
Bse8I GATNNNNATC 2 cut(s) 1407, 2655
BseAI TCCGGA 1 cut(s) 1965
BseBI CCWGG 6 cut(s) 23, 539, 1342, 1893, 2506, 2661
BseDI CCNNGG 9 cut(s) 22, 55, 70, 79, 109, 171, 538, 1606, 2505
BseGI GGATG 6 cut(s) 435, 1402, 1567, 2369, 2656, 2950
BseJI GATNNNNATC 2 cut(s) 1407, 2655
BseLI CCNNNNNNNGG 8 cut(s) 924, 1072, 1347, 1643, 1644, 2174, 2542, 2941
BseMI GCAATG 3 cut(s) 690, 1725, 1941
BseMII CTCAG 6 cut(s) 149, 720, 728, 824, 843, 887
BseNI ACTGG 5 cut(s) 972, 1018, 1060, 2426, 2818
BseRI GAGGAG 3 cut(s) 96, 99, 2135
BseX3I CGGCCG 2 cut(s) 28, 106
BseXI GCAGC 6 cut(s) 45, 892, 2020, 2221, 2224, 2284
BseYI CCCAGC 5 cut(s) 581, 1557, 2570, 2675, 2935
BsgI GTGCAG 1 cut(s) 2612
Bsh1236I CGCG 3 cut(s) 57, 93, 173
Bsh1285I CGRYCG 2 cut(s) 31, 109
BshFI GGCC 7 cut(s) 30, 45, 78, 108, 302, 537, 1881
BsiEI CGRYCG 2 cut(s) 31, 109
BsiHKCI CYCGRG 2 cut(s) 772, 1616
BsiSI CCGG 2 cut(s) 1238, 1966
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 8 cut(s) 924, 1072, 1347, 1643, 1644, 2174, 2542, 2941
BsmBI CGTCTC 1 cut(s) 1858
BsmFI GGGAC 1 cut(s) 146
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 7 cut(s) 30, 45, 78, 108, 302, 537, 1881
Bso31I GGTCTC 2 cut(s) 1125, 2247
BsoBI CYCGRG 2 cut(s) 772, 1616
Bsp13I TCCGGA 1 cut(s) 1965
Bsp1407I TGTACA 2 cut(s) 2150, 3007
BspANI GGCC 7 cut(s) 30, 45, 78, 108, 302, 537, 1881
BspCNI CTCAG 6 cut(s) 150, 721, 729, 823, 844, 886
BspEI TCCGGA 1 cut(s) 1965
BspFNI CGCG 3 cut(s) 57, 93, 173
BspLI GGNNCC 7 cut(s) 134, 135, 366, 409, 597, 1374, 2166
BspMAI CTGCAG 1 cut(s) 448
BspOI GCTAGC 1 cut(s) 1718
BspPI GGATC 9 cut(s) 1367, 1380, 1607, 2032, 2159, 2172, 2301, 2395, 2671
BspTI CTTAAG 1 cut(s) 638
BspTNI GGTCTC 2 cut(s) 1125, 2247
BsrBI CCGCTC 2 cut(s) 12, 236
BsrDI GCAATG 3 cut(s) 690, 1725, 1941
BsrGI TGTACA 2 cut(s) 2150, 3007
BsrI ACTGG 5 cut(s) 972, 1018, 1060, 2426, 2818
BssECI CCNNGG 9 cut(s) 22, 55, 70, 79, 109, 171, 538, 1606, 2505
BssNI GRCGYC 2 cut(s) 524, 1147
BssT1I CCWWGG 1 cut(s) 1606
Bst2UI CCWGG 6 cut(s) 23, 539, 1342, 1893, 2506, 2661
Bst4CI ACNGT 5 cut(s) 559, 607, 949, 1050, 1501
Bst6I CTCTTC 1 cut(s) 2599
BstACI GRCGYC 2 cut(s) 524, 1147
BstAFI CTTAAG 1 cut(s) 638
BstAUI TGTACA 2 cut(s) 2150, 3007
BstC8I GCNNGC 8 cut(s) 67, 147, 476, 498, 1716, 1875, 1879, 2734
BstDEI CTNAG 8 cut(s) 158, 626, 729, 737, 810, 852, 873, 2242
BstDSI CCRYGG 3 cut(s) 55, 70, 171
BstEII GGTNACC 1 cut(s) 2840
BstENI CCTNNNNNAGG 1 cut(s) 922
BstF5I GGATG 6 cut(s) 435, 1402, 1567, 2369, 2656, 2950
BstFNI CGCG 3 cut(s) 57, 93, 173
BstH2I RGCGCY 3 cut(s) 66, 127, 982
BstHHI GCGC 4 cut(s) 65, 126, 981, 2895
BstMCI CGRYCG 2 cut(s) 31, 109
BstNI CCWGG 6 cut(s) 23, 539, 1342, 1893, 2506, 2661
BstPI GGTNACC 1 cut(s) 2840
BstSCI CCNGG 7 cut(s) 21, 537, 1237, 1340, 1891, 2504, 2659
BstSFI CTRYAG 2 cut(s) 435, 444
BstUI CGCG 3 cut(s) 57, 93, 173
BstV1I GCAGC 6 cut(s) 45, 892, 2020, 2221, 2224, 2284
BstV2I GAAGAC 2 cut(s) 1281, 2732
BstX2I RGATCY 6 cut(s) 1153, 1372, 1612, 2164, 2306, 2470
BstYI RGATCY 6 cut(s) 1153, 1372, 1612, 2164, 2306, 2470
BstZI CGGCCG 2 cut(s) 28, 106
BsuRI GGCC 7 cut(s) 30, 45, 78, 108, 302, 537, 1881
BtgI CCRYGG 3 cut(s) 55, 70, 171
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 6 cut(s) 435, 1402, 1567, 2369, 2656, 2950
BtsI GCAGTG 1 cut(s) 2600
BtsIMutI CAGTG 3 cut(s) 525, 2335, 2600
Cac8I GCNNGC 8 cut(s) 67, 147, 476, 498, 1716, 1875, 1879, 2734
CaiI CAGNNNCTG 1 cut(s) 443
CciNI GCGGCCGC 1 cut(s) 28
CfoI GCGC 4 cut(s) 65, 126, 981, 2895
Cfr13I GGNCC 2 cut(s) 44, 133
Cfr42I CCGCGG 2 cut(s) 58, 174
CseI GACGC 2 cut(s) 99, 513
Csp6I GTAC 2 cut(s) 2151, 3008
CviQI GTAC 2 cut(s) 2151, 3008
DdeI CTNAG 8 cut(s) 158, 626, 729, 737, 810, 852, 873, 2242
DraI TTTAAA 2 cut(s) 1809, 2916
EaeI YGGCCR 4 cut(s) 28, 106, 535, 1879
EagI CGGCCG 2 cut(s) 28, 106
Eam1104I CTCTTC 1 cut(s) 2599
EarI CTCTTC 1 cut(s) 2599
EclXI CGGCCG 2 cut(s) 28, 106
Eco130I CCWWGG 1 cut(s) 1606
Eco31I GGTCTC 2 cut(s) 1125, 2247
Eco32I GATATC 1 cut(s) 2653
Eco47I GGWCC 1 cut(s) 133
Eco47III AGCGCT 2 cut(s) 125, 980
Eco52I CGGCCG 2 cut(s) 28, 106
Eco57I CTGAAG 2 cut(s) 501, 1836
Eco88I CYCGRG 2 cut(s) 772, 1616
Eco91I GGTNACC 1 cut(s) 2840
EcoNI CCTNNNNNAGG 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 2840
EcoRII CCWGG 6 cut(s) 21, 537, 1340, 1891, 2504, 2659
EcoRV GATATC 1 cut(s) 2653
EcoT14I CCWWGG 1 cut(s) 1606
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 1606
Esp3I CGTCTC 1 cut(s) 1858
FalI AAGNNNNNCTT 4 cut(s) 609, 641, 1361, 1393
FaqI GGGAC 1 cut(s) 146
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 2 cut(s) 277, 2332
FblI GTMKAC 2 cut(s) 434, 2496
FokI GGATG 6 cut(s) 442, 1409, 1574, 2376, 2643, 2957
FspBI CTAG 5 cut(s) 311, 821, 1271, 1715, 2952
GlaI GCGC 4 cut(s) 64, 125, 980, 2894
GsaI CCCAGC 5 cut(s) 585, 1561, 2574, 2679, 2939
GsuI CTGGAG 4 cut(s) 571, 1035, 1914, 2835
HaeII RGCGCY 3 cut(s) 66, 127, 982
HaeIII GGCC 7 cut(s) 30, 45, 78, 108, 302, 537, 1881
HapII CCGG 2 cut(s) 1238, 1966
HgaI GACGC 2 cut(s) 99, 513
HhaI GCGC 4 cut(s) 65, 126, 981, 2895
Hin1I GRCGYC 2 cut(s) 524, 1147
Hin6I GCGC 4 cut(s) 63, 124, 979, 2893
HinP1I GCGC 4 cut(s) 63, 124, 979, 2893
HincII GTYRAC 2 cut(s) 1053, 2497
HindII GTYRAC 2 cut(s) 1053, 2497
HindIII AAGCTT 5 cut(s) 498, 704, 1455, 2352, 2909
HpaI GTTAAC 1 cut(s) 1053
HpaII CCGG 2 cut(s) 1238, 1966
HphI GGTGA 4 cut(s) 788, 953, 2330, 2415
Hpy166II GTNNAC 8 cut(s) 435, 1053, 1469, 2153, 2497, 2683, 2900, 3010
Hpy8I GTNNAC 8 cut(s) 435, 1053, 1469, 2153, 2497, 2683, 2900, 3010
Hpy99I CGWCG 2 cut(s) 885, 1149
HpyAV CCTTC 9 cut(s) 337, 641, 655, 1145, 1341, 1363, 1665, 2354, 2855
HpyCH4III ACNGT 5 cut(s) 559, 607, 949, 1050, 1501
HpyCH4IV ACGT 6 cut(s) 259, 692, 838, 1147, 1247, 1278
HpyF3I CTNAG 8 cut(s) 158, 626, 729, 737, 810, 852, 873, 2242
HpySE526I ACGT 6 cut(s) 259, 692, 838, 1147, 1247, 1278
Hsp92I GRCGYC 2 cut(s) 524, 1147
HspAI GCGC 4 cut(s) 63, 124, 979, 2893
KflI GGGWCCC 1 cut(s) 133
Kpn2I TCCGGA 1 cut(s) 1965
Ksp22I TGATCA 2 cut(s) 277, 2332
KspAI GTTAAC 1 cut(s) 1053
KspI CCGCGG 2 cut(s) 58, 174
Lsp1109I GCAGC 6 cut(s) 45, 892, 2020, 2221, 2224, 2284
LweI GCATC 8 cut(s) 181, 1792, 1921, 2172, 2284, 2362, 2820, 2867
MaeI CTAG 5 cut(s) 311, 821, 1271, 1715, 2952
MaeII ACGT 6 cut(s) 259, 692, 838, 1147, 1247, 1278
MaeIII GTNAC 5 cut(s) 1575, 1901, 1950, 2428, 2840
MbiI CCGCTC 2 cut(s) 12, 236
MfeI CAATTG 1 cut(s) 2229
MflI RGATCY 6 cut(s) 1153, 1372, 1612, 2164, 2306, 2470
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 8 cut(s) 262, 1122, 1336, 1707, 1959, 2229, 2559, 2888
MluNI TGGCCA 1 cut(s) 537
MlyI GAGTC 6 cut(s) 462, 469, 1316, 1322, 1355, 2744
MmeI TCCRAC 4 cut(s) 39, 1912, 2002, 2828
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 1 cut(s) 331
MroI TCCGGA 1 cut(s) 1965
MroXI GAANNNNTTC 1 cut(s) 705
MscI TGGCCA 1 cut(s) 537
MslI CAYNNNNRTG 2 cut(s) 324, 2828
Msp20I TGGCCA 1 cut(s) 537
MspA1I CMGCKG 2 cut(s) 57, 173
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 2 cut(s) 1238, 1966
MspR9I CCNGG 7 cut(s) 23, 539, 1239, 1342, 1893, 2506, 2661
MunI CAATTG 1 cut(s) 2229
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 6 cut(s) 23, 539, 1342, 1893, 2506, 2661
MvnI CGCG 3 cut(s) 57, 93, 173
NciI CCSGG 1 cut(s) 1239
NheI GCTAGC 1 cut(s) 1714
NlaIV GGNNCC 7 cut(s) 134, 135, 366, 409, 597, 1374, 2166
NmeAIII GCCGAG 3 cut(s) 104, 134, 175
NotI GCGGCCGC 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 331
PctI GAATGC 1 cut(s) 713
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 9 cut(s) 424, 934, 961, 971, 1602, 1924, 2057, 2321, 2881
PflMI CCANNNNNTGG 3 cut(s) 1072, 2174, 2542
PfoI TCCNGGA 2 cut(s) 1340, 2659
PleI GAGTC 6 cut(s) 461, 468, 1315, 1321, 1355, 2744
PpsI GAGTC 6 cut(s) 461, 468, 1315, 1321, 1355, 2744
PpuMI RGGWCCY 1 cut(s) 133
Psp1406I AACGTT 1 cut(s) 1247
Psp5II RGGWCCY 1 cut(s) 133
Psp6I CCWGG 6 cut(s) 21, 537, 1340, 1891, 2504, 2659
PspEI GGTNACC 1 cut(s) 2840
PspFI CCCAGC 5 cut(s) 581, 1557, 2570, 2675, 2935
PspGI CCWGG 6 cut(s) 21, 537, 1340, 1891, 2504, 2659
PspN4I GGNNCC 7 cut(s) 134, 135, 366, 409, 597, 1374, 2166
PspPI GGNCC 2 cut(s) 44, 133
PspPPI RGGWCCY 1 cut(s) 133
PstI CTGCAG 1 cut(s) 448
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 6 cut(s) 1153, 1372, 1612, 2164, 2306, 2470
RsaI GTAC 2 cut(s) 2152, 3009
RsaNI GTAC 2 cut(s) 2151, 3008
RseI CAYNNNNRTG 2 cut(s) 324, 2828
SacII CCGCGG 2 cut(s) 58, 174
SalI GTCGAC 1 cut(s) 2495
Sau96I GGNCC 2 cut(s) 44, 133
SchI GAGTC 6 cut(s) 462, 469, 1316, 1322, 1355, 2744
ScrFI CCNGG 7 cut(s) 23, 539, 1239, 1342, 1893, 2506, 2661
SfaNI GCATC 8 cut(s) 181, 1792, 1921, 2172, 2284, 2362, 2820, 2867
SfcI CTRYAG 2 cut(s) 435, 444
Sfr303I CCGCGG 2 cut(s) 58, 174
SgrBI CCGCGG 2 cut(s) 58, 174
SinI GGWCC 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 324, 2828
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 8 cut(s) 262, 1122, 1336, 1707, 1959, 2229, 2559, 2888
SspI AATATT 1 cut(s) 1677
SspMI CTAG 5 cut(s) 311, 821, 1271, 1715, 2952
StyD4I CCNGG 7 cut(s) 21, 537, 1237, 1340, 1891, 2504, 2659
StyI CCWWGG 1 cut(s) 1606
TaaI ACNGT 5 cut(s) 559, 607, 949, 1050, 1501
TaiI ACGT 6 cut(s) 262, 695, 841, 1150, 1250, 1281
TaqI TCGA 9 cut(s) 964, 1144, 1305, 2082, 2251, 2480, 2496, 2649, 2904
TasI AATT 8 cut(s) 262, 1122, 1336, 1707, 1959, 2229, 2559, 2888
TatI WGTACW 2 cut(s) 2150, 3007
TfiI GAWTC 9 cut(s) 424, 934, 961, 971, 1602, 1924, 2057, 2321, 2881
TscAI CASTG 3 cut(s) 525, 2335, 2600
TseI GCWGC 6 cut(s) 33, 905, 2008, 2234, 2237, 2297
TspDTI ATGAA 9 cut(s) 309, 321, 392, 674, 1095, 1104, 1115, 1615, 2837
TspRI CASTG 3 cut(s) 525, 2335, 2600
Van91I CCANNNNNTGG 3 cut(s) 1072, 2174, 2542
Vha464I CTTAAG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 922
XapI RAATTY 2 cut(s) 1336, 1959
XbaI TCTAGA 2 cut(s) 820, 1270
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 2 cut(s) 434, 2496
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 5 cut(s) 311, 821, 1271, 1715, 2952
ZraI GACGTC 1 cut(s) 1148
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.