Rmu_sc0000114.1_g000014

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000114.1
OG0007834
Physical Location & Seq
Forward (+)
69989 .. 78153
8165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000114.1_g000014.1.cds

Sequence Viewer

Length: 3048 bp
atgtgttgtttgctcttaactttgcgcgcaaccaccaaccactttctccccactcacttcctcgccgctccacgactcctcacttttcgctctgccatctcaactctcgccatgtccagccgctccaacttccagggtggccgcagccgaggcggccctaactcccgcggcggcgctcgccgtggaggaggtggtcgcggcggcggaggccgaggaggaggaggacgcggcggcggcggcggccgaggcggcgaacagcgctggtgggaccccgtgtggcgagcggagcgactgaggcaacaagccgccgagatggaagttcttgatgcgagtgagttttgggggaagatggagcagttcagaagcggagcggagcaggagatgataataagacgtaattttagtcggcatgatcagcaaaccctatatgaaatggccgatgaactagggcttcacttccatgcatataataaggggaaggctcttgtggtcagcaaggttcctctgccagattatcgggcagaccttgatgatgttcatggttccacacaaaaagagattcggatgtctacagagactgcagagagagtcgggagtcttttgcaaagctcgcaggggcagggaagtaagcaagcttttggtaatgttgcttcagtggcgtctgggtctggccaggggaacaaactaacaactgttagtgaaaatacaaccaaacccagctccagcttggagcctgatactgttaaggagaaagagaaacttagccttcaacttaaggagtcgcaggaaaaaatgaaggtaagtaatagtcttaaagcaatgctgacattccgagaaaagcttcctgcattcaatgtgaagtctgagttcctgagagctgtttctgaaaatcaggtgttggtagtctcgggtgagacaggttgtggcaaaacaacacaacttcctcagtttattctagaagatgagatttcacgtctgcatggtgctgagtgcaacataatatgcactcagcctcgccgtatatctgccgtgtctgttgctgctcgtatatcctctgaaaggggagagaatcttggtgaaactgttggttatcagattcgactggaatcaaagcgctctgctcaaacaagattgctattttgcacaactggagtattacttcgtcagttggttcaagacccacagttaactggcgtgagccatttgttggttgatgaaatccatgaaagaggcatgaacgaggactttttactaataattttgcgagaccttcttcctcgacgtccagatctacgacttattctaatgagtgctaccatcaatgctgacttgttttccaaatactttggaaatgccccaaaaatacatataccgggattaacgttccctgtggcagagctatttctagaagacgttttggagaaaactcattatatagtcaagtcagagtccgacaaaatggagaggggaaattccaggagaaggagacagcaagactccaagaaggatcccttaactgaactatttgaggatgctgatattgatcttcactacaaaagttacagcacatctacaagaaagtctctagaagcttggtctggttcacagcttgatttgggtctggtggaggcaactgtggaacatatatgtcgctatgagagagatggagcagttcttgttttccttacaggctgggatgatatatcaaagttacttgacaaaattaagggaaatagattccttggagatcctgagaagttcatggttcttcccctgcatgggtcaatgcctactattaatcaacgagaaatatttgatcgtcctcccactaccaaaagaaaaattgtgctagcaacaaacattgcagagagcagtattaccatagatgatgtcgtgtatgtcatagactgtggaaaggcaaaggaaacaagttatgatgctttaaacaagttggcttgtctattaccatcatggatttcaaaggcttcagcacatcagagacgagggcgtgctggccgtgtccaacctggagtttgttacagactatacccaaaaatgattcatgatgcaatgctccaatatcagttacctgaaattctccggacacctttgcaagagttatgcctccatataaagagtttgcagcttggagctgttggatcatttttggcaaaggcacttcaaccaccagattctcttgcagttcaaaatgccatcgaacttctcaaaactattggagctttagatgatagggaggagcttactccgcttggtcgccatctttgtacactaccattggatccaaacattggaaagatgcttttaatgggttctatctttcaatgcctcaatcctgccttaacaattgctgctgccctagctcatcgagacccatttatcctaccaatagataggaaagaggaagctgatgctgcaaaaagatcctttgctggtgattctttcagtgatcacatagcagttgttaaagcttttgaaggatggaaagatgcaaaacataatggatcaggaaaatcattctgttgggataactttttatcaccagtaaccttgcagatgatggaggatatgaggttgcagtttgtagatctattatcgaacattggctttgtcaacaaatccaggggtgctaacgcttacaaccaatacagccatgacttggagatggtgtctgcaattctttgtgctgggctttacccaaatgttgtgcagtgtaaaagaagaggaaagcggacagcattctacaccaaagaagttggtaaagtcgatatccatcctggatcagtcaatgctggggttcacttattccctctgccttacatggtatatagtgaaaaagtgaagacagccagcatttacatcagagactctacaaatatatcagattattctttgcttttgtttggtggtaatctcattccaaccaaaactggagagggcattgagatgcttggaggttaccttcatttctctgcatcaaagagtgttttggagttgattcgggtaacgcctcttgggatctcatgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1015

Amino Acids

112.5

Weight (kDa)

8.83

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1285
AccB7I CCANNNNNTGG 3 cut(s) 1207, 2309, 2677
AccBSI CCGCTC 4 cut(s) 68, 123, 284, 371
AccI GTMKAC 1 cut(s) 569
AccII CGCG 4 cut(s) 27, 168, 198, 228
AccIII TCCGGA 1 cut(s) 2100
AclI AACGTT 1 cut(s) 1382
AcoI YGGCCR 5 cut(s) 139, 241, 435, 670, 2014
AcsI RAATTY 2 cut(s) 1471, 2094
AcuI CTGAAG 2 cut(s) 636, 1971
AcyI GRCGYC 2 cut(s) 659, 1282
AfaI GTAC 1 cut(s) 2287
AfeI AGCGCT 2 cut(s) 260, 1115
AfiI CCNNNNNNNGG 7 cut(s) 1059, 1207, 1482, 1778, 1779, 2309, 2677
AflII CTTAAG 1 cut(s) 773
AgsI TTSAA 8 cut(s) 770, 853, 1175, 1980, 2183, 2207, 2342, 2495
AjiI CACGTC 1 cut(s) 974
AjnI CCWGG 6 cut(s) 132, 672, 1475, 2026, 2639, 2794
Alw26I GTCTC 9 cut(s) 569, 908, 910, 1260, 1480, 1587, 1993, 2382, 2877
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 907
Aor13HI TCCGGA 1 cut(s) 2100
Aor51HI AGCGCT 2 cut(s) 260, 1115
AoxI GGCC 7 cut(s) 139, 154, 208, 241, 435, 670, 2014
ApeKI GCWGC 6 cut(s) 144, 1040, 2143, 2369, 2372, 2432
ApoI RAATTY 2 cut(s) 1471, 2094
AseI ATTAAT 1 cut(s) 1797
Asp700I GAANNNNTTC 1 cut(s) 840
AspLEI GCGC 5 cut(s) 27, 29, 176, 261, 1116
AspS9I GGNCC 2 cut(s) 155, 268
AsuC2I CCSGG 1 cut(s) 1374
AsuHPI GGTGA 4 cut(s) 923, 1088, 2465, 2550
AsuNHI GCTAGC 1 cut(s) 1849
AvaI CYCGRG 1 cut(s) 907
AvaII GGWCC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 672
BamHI GGATCC 2 cut(s) 1507, 2299
BarI GAAGNNNNNNTAC 2 cut(s) 634, 666
BbsI GAAGAC 2 cut(s) 1416, 2867
BbvI GCAGC 6 cut(s) 156, 1027, 2155, 2356, 2359, 2419
BceAI ACGGC 4 cut(s) 165, 1002, 1013, 2001
BciT130I CCWGG 6 cut(s) 134, 674, 1477, 2028, 2641, 2796
BclI TGATCA 2 cut(s) 412, 2467
BcnI CCSGG 1 cut(s) 1374
BcoDI GTCTC 9 cut(s) 569, 908, 910, 1260, 1480, 1587, 1993, 2382, 2877
BfaI CTAG 6 cut(s) 446, 956, 1406, 1586, 1850, 2378
BfmI CTRYAG 2 cut(s) 570, 579
BfoI RGCGCY 3 cut(s) 177, 262, 1117
BfrI CTTAAG 1 cut(s) 773
BglI GCCNNNNNGGC 2 cut(s) 153, 249
BglII AGATCT 2 cut(s) 1288, 2605
Bme1390I CCNGG 7 cut(s) 134, 674, 1374, 1477, 2028, 2641, 2796
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 907
BmgBI CACGTC 1 cut(s) 974
BmgT120I GGNCC 2 cut(s) 155, 268
BmiI GGNNCC 7 cut(s) 269, 270, 501, 544, 732, 1509, 2301
BmrFI CCNGG 7 cut(s) 134, 674, 1374, 1477, 2028, 2641, 2796
BmsI GCATC 9 cut(s) 316, 1522, 1927, 2056, 2307, 2419, 2497, 2955, 3002
BmtI GCTAGC 1 cut(s) 1853
BpiI GAAGAC 2 cut(s) 1416, 2867
BpmI CTGGAG 4 cut(s) 706, 1170, 2049, 2970
BpuMI CCSGG 1 cut(s) 1374
BsaBI GATNNNNATC 2 cut(s) 1542, 2790
BsaHI GRCGYC 2 cut(s) 659, 1282
BsaI GGTCTC 2 cut(s) 1260, 2382
BsaJI CCNNGG 9 cut(s) 133, 148, 166, 181, 211, 244, 673, 1741, 2640
BsaWI WCCGGW 1 cut(s) 2100
BsaXI ACNNNNNCTCC 2 cut(s) 2672, 2702
Bsc4I CCNNNNNNNGG 7 cut(s) 1059, 1207, 1482, 1778, 1779, 2309, 2677
Bse1I ACTGG 5 cut(s) 1107, 1153, 1195, 2561, 2953
Bse3DI GCAATG 3 cut(s) 825, 1860, 2076
Bse8I GATNNNNATC 2 cut(s) 1542, 2790
BseAI TCCGGA 1 cut(s) 2100
BseBI CCWGG 6 cut(s) 134, 674, 1477, 2028, 2641, 2796
BseDI CCNNGG 9 cut(s) 133, 148, 166, 181, 211, 244, 673, 1741, 2640
BseGI GGATG 5 cut(s) 570, 1537, 1702, 2504, 2791
BseJI GATNNNNATC 2 cut(s) 1542, 2790
BseLI CCNNNNNNNGG 7 cut(s) 1059, 1207, 1482, 1778, 1779, 2309, 2677
BseMI GCAATG 3 cut(s) 825, 1860, 2076
BseMII CTCAG 7 cut(s) 284, 855, 863, 959, 978, 1022, 1743
BseNI ACTGG 5 cut(s) 1107, 1153, 1195, 2561, 2953
BsePI GCGCGC 1 cut(s) 25
BseRI GAGGAG 6 cut(s) 68, 201, 228, 231, 234, 2270
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 6 cut(s) 156, 1027, 2155, 2356, 2359, 2419
BseYI CCCAGC 4 cut(s) 716, 1692, 2705, 2810
BsgI GTGCAG 1 cut(s) 2747
Bsh1236I CGCG 4 cut(s) 27, 168, 198, 228
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 7 cut(s) 141, 156, 210, 243, 437, 672, 2016
BsiEI CGRYCG 1 cut(s) 244
BsiHKCI CYCGRG 1 cut(s) 907
BsiSI CCGG 2 cut(s) 1373, 2101
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 7 cut(s) 1059, 1207, 1482, 1778, 1779, 2309, 2677
BsmAI GTCTC 9 cut(s) 569, 908, 910, 1260, 1480, 1587, 1993, 2382, 2877
BsmBI CGTCTC 1 cut(s) 1993
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 2 cut(s) 848, 2756
BsnI GGCC 7 cut(s) 141, 156, 210, 243, 437, 672, 2016
Bso31I GGTCTC 2 cut(s) 1260, 2382
BsoBI CYCGRG 1 cut(s) 907
Bsp13I TCCGGA 1 cut(s) 2100
Bsp1407I TGTACA 1 cut(s) 2285
BspANI GGCC 7 cut(s) 141, 156, 210, 243, 437, 672, 2016
BspCNI CTCAG 7 cut(s) 285, 856, 864, 958, 979, 1021, 1744
BspEI TCCGGA 1 cut(s) 2100
BspFNI CGCG 4 cut(s) 27, 168, 198, 228
BspHI TCATGA 1 cut(s) 2062
BspLI GGNNCC 7 cut(s) 269, 270, 501, 544, 732, 1509, 2301
BspMAI CTGCAG 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 1853
BspTI CTTAAG 1 cut(s) 773
BspTNI GGTCTC 2 cut(s) 1260, 2382
BsrBI CCGCTC 4 cut(s) 68, 123, 284, 371
BsrDI GCAATG 3 cut(s) 825, 1860, 2076
BsrGI TGTACA 1 cut(s) 2285
BsrI ACTGG 5 cut(s) 1107, 1153, 1195, 2561, 2953
BssECI CCNNGG 9 cut(s) 133, 148, 166, 181, 211, 244, 673, 1741, 2640
BssHII GCGCGC 1 cut(s) 25
BssNI GRCGYC 2 cut(s) 659, 1282
BssT1I CCWWGG 1 cut(s) 1741
Bst2UI CCWGG 6 cut(s) 134, 674, 1477, 2028, 2641, 2796
Bst4CI ACNGT 6 cut(s) 694, 742, 1084, 1185, 1636, 1910
Bst6I CTCTTC 1 cut(s) 2734
BstACI GRCGYC 2 cut(s) 659, 1282
BstAFI CTTAAG 1 cut(s) 773
BstAUI TGTACA 1 cut(s) 2285
BstC8I GCNNGC 9 cut(s) 27, 178, 282, 611, 633, 1851, 2010, 2014, 2869
BstDEI CTNAG 8 cut(s) 293, 761, 864, 872, 945, 987, 1008, 1752
BstDSI CCRYGG 2 cut(s) 166, 181
BstEII GGTNACC 1 cut(s) 2975
BstENI CCTNNNNNAGG 1 cut(s) 1057
BstF5I GGATG 5 cut(s) 570, 1537, 1702, 2504, 2791
BstFNI CGCG 4 cut(s) 27, 168, 198, 228
BstH2I RGCGCY 3 cut(s) 177, 262, 1117
BstHHI GCGC 5 cut(s) 27, 29, 176, 261, 1116
BstMAI GTCTC 9 cut(s) 569, 908, 910, 1260, 1480, 1587, 1993, 2382, 2877
BstMCI CGRYCG 1 cut(s) 244
BstNI CCWGG 6 cut(s) 134, 674, 1477, 2028, 2641, 2796
BstPI GGTNACC 1 cut(s) 2975
BstSCI CCNGG 7 cut(s) 132, 672, 1372, 1475, 2026, 2639, 2794
BstSFI CTRYAG 2 cut(s) 570, 579
BstUI CGCG 4 cut(s) 27, 168, 198, 228
BstV1I GCAGC 6 cut(s) 156, 1027, 2155, 2356, 2359, 2419
BstV2I GAAGAC 2 cut(s) 1416, 2867
BstX2I RGATCY 7 cut(s) 1288, 1507, 1747, 2299, 2441, 2605, 3036
BstYI RGATCY 7 cut(s) 1288, 1507, 1747, 2299, 2441, 2605, 3036
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 7 cut(s) 141, 156, 210, 243, 437, 672, 2016
BtgI CCRYGG 2 cut(s) 166, 181
BtrI CACGTC 1 cut(s) 974
BtsCI GGATG 5 cut(s) 570, 1537, 1702, 2504, 2791
BtsI GCAGTG 1 cut(s) 2735
BtsIMutI CAGTG 3 cut(s) 660, 2470, 2735
Cac8I GCNNGC 9 cut(s) 27, 178, 282, 611, 633, 1851, 2010, 2014, 2869
CaiI CAGNNNCTG 1 cut(s) 578
CciI TCATGA 1 cut(s) 2062
CfoI GCGC 5 cut(s) 27, 29, 176, 261, 1116
Cfr13I GGNCC 2 cut(s) 155, 268
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 2 cut(s) 234, 648
Csp6I GTAC 1 cut(s) 2286
CviQI GTAC 1 cut(s) 2286
DdeI CTNAG 8 cut(s) 293, 761, 864, 872, 945, 987, 1008, 1752
DraI TTTAAA 1 cut(s) 1944
EaeI YGGCCR 5 cut(s) 139, 241, 435, 670, 2014
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 2734
EarI CTCTTC 1 cut(s) 2734
EciI GGCGGA 1 cut(s) 219
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 1741
Eco31I GGTCTC 2 cut(s) 1260, 2382
Eco32I GATATC 1 cut(s) 2788
Eco47I GGWCC 1 cut(s) 268
Eco47III AGCGCT 2 cut(s) 260, 1115
Eco52I CGGCCG 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 636, 1971
Eco88I CYCGRG 1 cut(s) 907
Eco91I GGTNACC 1 cut(s) 2975
EcoNI CCTNNNNNAGG 1 cut(s) 1057
EcoO109I RGGNCCY 1 cut(s) 268
EcoO65I GGTNACC 1 cut(s) 2975
EcoRII CCWGG 6 cut(s) 132, 672, 1475, 2026, 2639, 2794
EcoRV GATATC 1 cut(s) 2788
EcoT14I CCWWGG 1 cut(s) 1741
EcoT22I ATGCAT 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 1741
Esp3I CGTCTC 1 cut(s) 1993
FalI AAGNNNNNCTT 4 cut(s) 744, 776, 1496, 1528
FaqI GGGAC 1 cut(s) 281
FauI CCCGC 1 cut(s) 173
FbaI TGATCA 2 cut(s) 412, 2467
FblI GTMKAC 1 cut(s) 569
FokI GGATG 5 cut(s) 577, 1544, 1709, 2511, 2778
FspBI CTAG 6 cut(s) 446, 956, 1406, 1586, 1850, 2378
GlaI GCGC 5 cut(s) 26, 28, 175, 260, 1115
GsaI CCCAGC 4 cut(s) 720, 1696, 2709, 2814
GsuI CTGGAG 4 cut(s) 706, 1170, 2049, 2970
HaeII RGCGCY 3 cut(s) 177, 262, 1117
HaeIII GGCC 7 cut(s) 141, 156, 210, 243, 437, 672, 2016
HapII CCGG 2 cut(s) 1373, 2101
HgaI GACGC 2 cut(s) 234, 648
HhaI GCGC 5 cut(s) 27, 29, 176, 261, 1116
Hin1I GRCGYC 2 cut(s) 659, 1282
Hin6I GCGC 5 cut(s) 25, 27, 174, 259, 1114
HinP1I GCGC 5 cut(s) 25, 27, 174, 259, 1114
HincII GTYRAC 2 cut(s) 1188, 2632
HindII GTYRAC 2 cut(s) 1188, 2632
HindIII AAGCTT 4 cut(s) 633, 839, 1590, 2487
HpaI GTTAAC 1 cut(s) 1188
HpaII CCGG 2 cut(s) 1373, 2101
HphI GGTGA 4 cut(s) 923, 1088, 2465, 2550
Hpy166II GTNNAC 6 cut(s) 570, 1188, 1604, 2288, 2632, 2818
Hpy8I GTNNAC 6 cut(s) 570, 1188, 1604, 2288, 2632, 2818
Hpy99I CGWCG 1 cut(s) 1284
HpyAV CCTTC 8 cut(s) 472, 776, 790, 1280, 1476, 1498, 2489, 2990
HpyCH4III ACNGT 6 cut(s) 694, 742, 1084, 1185, 1636, 1910
HpyCH4IV ACGT 5 cut(s) 394, 973, 1282, 1382, 1413
HpyF3I CTNAG 8 cut(s) 293, 761, 864, 872, 945, 987, 1008, 1752
HpySE526I ACGT 5 cut(s) 394, 973, 1282, 1382, 1413
Hsp92I GRCGYC 2 cut(s) 659, 1282
HspAI GCGC 5 cut(s) 25, 27, 174, 259, 1114
KflI GGGWCCC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 2100
Ksp22I TGATCA 2 cut(s) 412, 2467
KspAI GTTAAC 1 cut(s) 1188
KspI CCGCGG 1 cut(s) 169
Lsp1109I GCAGC 6 cut(s) 156, 1027, 2155, 2356, 2359, 2419
LweI GCATC 9 cut(s) 316, 1522, 1927, 2056, 2307, 2419, 2497, 2955, 3002
MaeI CTAG 6 cut(s) 446, 956, 1406, 1586, 1850, 2378
MaeII ACGT 5 cut(s) 394, 973, 1282, 1382, 1413
MaeIII GTNAC 7 cut(s) 1559, 1710, 2036, 2085, 2563, 2975, 3022
MbiI CCGCTC 4 cut(s) 68, 123, 284, 371
MboII GAAGA 8 cut(s) 358, 971, 1265, 1421, 1538, 1760, 2751, 2872
MfeI CAATTG 1 cut(s) 2364
MflI RGATCY 7 cut(s) 1288, 1507, 1747, 2299, 2441, 2605, 3036
MlsI TGGCCA 1 cut(s) 672
MluCI AATT 8 cut(s) 397, 1257, 1471, 1722, 1842, 2094, 2364, 2694
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 7 cut(s) 69, 597, 604, 788, 1457, 1490, 2879
MmeI TCCRAC 5 cut(s) 150, 1476, 2047, 2137, 2963
Mox20I TGGCCA 1 cut(s) 672
Mph1103I ATGCAT 1 cut(s) 466
MroI TCCGGA 1 cut(s) 2100
MroXI GAANNNNTTC 1 cut(s) 840
MscI TGGCCA 1 cut(s) 672
MslI CAYNNNNRTG 2 cut(s) 459, 2963
Msp20I TGGCCA 1 cut(s) 672
MspA1I CMGCKG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 2 cut(s) 1373, 2101
MspR9I CCNGG 7 cut(s) 134, 674, 1374, 1477, 2028, 2641, 2796
MunI CAATTG 1 cut(s) 2364
Mva1269I GAATGC 2 cut(s) 848, 2756
MvaI CCWGG 6 cut(s) 134, 674, 1477, 2028, 2641, 2796
MvnI CGCG 4 cut(s) 27, 168, 198, 228
NciI CCSGG 1 cut(s) 1374
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 7 cut(s) 269, 270, 501, 544, 732, 1509, 2301
NmeAIII GCCGAG 4 cut(s) 173, 236, 269, 334
NsiI ATGCAT 1 cut(s) 466
PagI TCATGA 1 cut(s) 2062
PauI GCGCGC 1 cut(s) 25
PctI GAATGC 2 cut(s) 848, 2756
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 9 cut(s) 559, 1069, 1096, 1106, 1737, 2059, 2192, 2456, 3016
PflMI CCANNNNNTGG 3 cut(s) 1207, 2309, 2677
PfoI TCCNGGA 2 cut(s) 1475, 2794
PleI GAGTC 7 cut(s) 69, 596, 603, 787, 1456, 1490, 2879
PpsI GAGTC 7 cut(s) 69, 596, 603, 787, 1456, 1490, 2879
PpuMI RGGWCCY 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 1797
Psp1406I AACGTT 1 cut(s) 1382
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 6 cut(s) 132, 672, 1475, 2026, 2639, 2794
PspEI GGTNACC 1 cut(s) 2975
PspFI CCCAGC 4 cut(s) 716, 1692, 2705, 2810
PspGI CCWGG 6 cut(s) 132, 672, 1475, 2026, 2639, 2794
PspN4I GGNNCC 7 cut(s) 269, 270, 501, 544, 732, 1509, 2301
PspPI GGNCC 2 cut(s) 155, 268
PspPPI RGGWCCY 1 cut(s) 268
PstI CTGCAG 1 cut(s) 583
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 7 cut(s) 1288, 1507, 1747, 2299, 2441, 2605, 3036
PteI GCGCGC 1 cut(s) 25
RsaI GTAC 1 cut(s) 2287
RsaNI GTAC 1 cut(s) 2286
RseI CAYNNNNRTG 2 cut(s) 459, 2963
SacII CCGCGG 1 cut(s) 169
Sau96I GGNCC 2 cut(s) 155, 268
SchI GAGTC 7 cut(s) 69, 597, 604, 788, 1457, 1490, 2879
ScrFI CCNGG 7 cut(s) 134, 674, 1374, 1477, 2028, 2641, 2796
SfaNI GCATC 9 cut(s) 316, 1522, 1927, 2056, 2307, 2419, 2497, 2955, 3002
SfcI CTRYAG 2 cut(s) 570, 579
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 2 cut(s) 459, 2963
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 8 cut(s) 397, 1257, 1471, 1722, 1842, 2094, 2364, 2694
SspI AATATT 1 cut(s) 1812
SspMI CTAG 6 cut(s) 446, 956, 1406, 1586, 1850, 2378
StyD4I CCNGG 7 cut(s) 132, 672, 1372, 1475, 2026, 2639, 2794
StyI CCWWGG 1 cut(s) 1741
TaaI ACNGT 6 cut(s) 694, 742, 1084, 1185, 1636, 1910
TaiI ACGT 5 cut(s) 397, 976, 1285, 1385, 1416
TaqI TCGA 6 cut(s) 1099, 1279, 2217, 2386, 2615, 2784
TasI AATT 8 cut(s) 397, 1257, 1471, 1722, 1842, 2094, 2364, 2694
TatI WGTACW 1 cut(s) 2285
TfiI GAWTC 9 cut(s) 559, 1069, 1096, 1106, 1737, 2059, 2192, 2456, 3016
TscAI CASTG 3 cut(s) 660, 2470, 2735
TseI GCWGC 6 cut(s) 144, 1040, 2143, 2369, 2372, 2432
TspRI CASTG 3 cut(s) 660, 2470, 2735
Van91I CCANNNNNTGG 3 cut(s) 1207, 2309, 2677
Vha464I CTTAAG 1 cut(s) 773
VpaK11BI GGWCC 1 cut(s) 268
VspI ATTAAT 1 cut(s) 1797
XagI CCTNNNNNAGG 1 cut(s) 1057
XapI RAATTY 2 cut(s) 1471, 2094
XbaI TCTAGA 3 cut(s) 955, 1405, 1585
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 6 cut(s) 446, 956, 1406, 1586, 1850, 2378
ZraI GACGTC 1 cut(s) 1283
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.