Rw3G018580

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007834
Physical Location & Seq
Reverse (-)
20813576 .. 20821949
8374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018580.1

Sequence Viewer

Length: 3117 bp
ATGTGTTGTTTGCTCTTAACTTTGCGCGCAACCACCAACCACTTTCTCCCCACTCACTTCCTCGCCGCTCCACGACTCCTCACTTTTCGCTCTGCCATCTCAACTCTCGCCATGTCCAGCCGCTCCAACTTCCAGGGTGGCCGCAGCCGAGGCGGCCCTAACTCCAGCGGCGGCGCTCGCCGTGGAGGCGGTGGTCGCGGCGGCGGAGGCCGAGGAGGCGAACAGCGCTGGTGGGACCCCGTGTGGCGAGCGGAGCGACTGAGGCAACAAGCCGCCGAGATGGAAGTTCTTGATGCGAGTGAGTATTGGGGGAAGATGGAGCAGTTCAGAAGCGGAGCGGAGCAGGAGATGATAATAAGACGTAATTTTAGTCGGCATGATCAGCAAACCCTATATGAAATGGCCGATGAACTAGGGCTTCACTTCCATGCATATAATAAGGGGAAGGCTCTTGTGGTCAGCAAGGTTCCTCTGCCAGATTATCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGGATGTCTACAGAGACTGCAGAGAGAGTTGGGAGTCTTTTGCAAAGCTCGCAGGGGCAGGGAAGTAAGCAAGCTTTTGGTAATGTTGCTTCAGTGGCGTCTGGGTCTGGCCAGGGGAACAAACTAACAACTGTTAGTGAAAAAACAACCAAACCCAGCTCCAGCTTGGAGCCTGATACTGTTAAGGAGAAAGAGAAACTTAGCCTTCAACTTAAGGAGTCGCAGGAAAAAATGAAGGTAAGTAATAGTCTTAAAGCAATGCTGACATTCCGAGAAAAGCTTCCTGCATTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGAGTGCAACATAATATGCACTCAGCCTCGCCGTATATCTGCCGTGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACAACTGGAGTATTACTTCGTCAGTTGGTTCAAGACCCACAGTTAACTGGCGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAACGAGGACTTTTTACTAATAATTTTGCGAGACCTTCTTCCTCGACGTCCAGATCTACGACTTATTCTAATGAGTGCTACCATCAATGCTGACTTGTTTTCCAAATACTTTGGAAATGCCCCAAAAATACATATACCGGGATTAACGTTCCCTGTGGCAGAGCTATTTCTAGAAGACGTTTTGGAGAAAACTCATTATATAGTCAAGTCAGAGTCCGACAAGATGGAGAGGGGAAATTCCAGGAGAAGGAGACAGCAAGACTCCAAGAAGGATCCCTTAACTGAACTATTTGAGGATGCTGATATTGATCTTCACTACAAAAGTTACAGCACATCTACAAGAAAGTCTCTAGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTCTGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACAGGCTGGGATGATATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCTGAGAAGTTCATGGTTCTTCCCCTGCATGGGTCAATGCCTACTATTAATCAACGAGAAATATTTGATCGTCCTCCCACTACCAAAAGAAAAATTGTGCTAGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGTGGAAAGGCAAAGGAAACAAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATACCCAAAAATGATTCATGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTTTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCCTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGATCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTCAACAAATCCAGGGGTGCTAACGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTTGGTAAAGTCGATATCCATCCTGGATCAGTCAATGCTGGGGTTCACTTATTCCCTCTGCCTTACATGGTATATAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTTGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTATTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1038

Amino Acids

115.52

Weight (kDa)

8.78

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 277 - 431 3e-09 DEAD/DEAH box helicase
Helicase_C PF00271 536 - 663 2.1e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 727 - 755 1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 756 - 815 2.4e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 886 - 966 1e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 976 - 1033 1.5e-20 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1252
AccB7I CCANNNNNTGG 3 cut(s) 1174, 2276, 2644
AccBSI CCGCTC 4 cut(s) 68, 123, 251, 338
AccI GTMKAC 1 cut(s) 536
AccII CGCG 2 cut(s) 27, 198
AccIII TCCGGA 1 cut(s) 2067
AclI AACGTT 1 cut(s) 1349
AclWI GGATC 9 cut(s) 1469, 1482, 1709, 2134, 2261, 2274, 2403, 2497, 2773
AcoI YGGCCR 4 cut(s) 139, 402, 637, 1981
AcsI RAATTY 2 cut(s) 1438, 2061
AcuI CTGAAG 2 cut(s) 603, 1938
AcyI GRCGYC 2 cut(s) 626, 1249
AfaI GTAC 2 cut(s) 2254, 3111
AfeI AGCGCT 2 cut(s) 227, 1082
AfiI CCNNNNNNNGG 8 cut(s) 1026, 1174, 1449, 1745, 1746, 2276, 2644, 3043
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 8 cut(s) 737, 820, 1142, 1947, 2150, 2174, 2309, 2462
AjiI CACGTC 1 cut(s) 941
AjnI CCWGG 6 cut(s) 132, 639, 1442, 1993, 2606, 2761
AlwI GGATC 9 cut(s) 1469, 1482, 1709, 2134, 2261, 2274, 2403, 2497, 2773
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 874
Aor13HI TCCGGA 1 cut(s) 2067
Aor51HI AGCGCT 2 cut(s) 227, 1082
AoxI GGCC 6 cut(s) 139, 154, 208, 402, 637, 1981
ApeKI GCWGC 6 cut(s) 144, 1007, 2110, 2336, 2339, 2399
ApoI RAATTY 2 cut(s) 1438, 2061
AseI ATTAAT 1 cut(s) 1764
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 6 cut(s) 27, 29, 176, 228, 1083, 2997
AspS9I GGNCC 2 cut(s) 155, 235
AsuC2I CCSGG 1 cut(s) 1341
AsuHPI GGTGA 4 cut(s) 890, 1055, 2432, 2517
AsuNHI GCTAGC 1 cut(s) 1816
AvaI CYCGRG 1 cut(s) 874
AvaII GGWCC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 639
BamHI GGATCC 2 cut(s) 1474, 2266
BarI GAAGNNNNNNTAC 2 cut(s) 601, 633
BbsI GAAGAC 2 cut(s) 1383, 2834
BbvI GCAGC 6 cut(s) 156, 994, 2122, 2323, 2326, 2386
BceAI ACGGC 5 cut(s) 165, 969, 980, 1968, 2982
BciT130I CCWGG 6 cut(s) 134, 641, 1444, 1995, 2608, 2763
BclI TGATCA 2 cut(s) 379, 2434
BcnI CCSGG 1 cut(s) 1341
BfaI CTAG 6 cut(s) 413, 923, 1373, 1553, 1817, 2345
BfmI CTRYAG 2 cut(s) 537, 546
BfoI RGCGCY 3 cut(s) 177, 229, 1084
BfrI CTTAAG 1 cut(s) 740
BglI GCCNNNNNGGC 3 cut(s) 153, 186, 216
BglII AGATCT 2 cut(s) 1255, 2572
Bme1390I CCNGG 7 cut(s) 134, 641, 1341, 1444, 1995, 2608, 2763
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 874
BmgBI CACGTC 1 cut(s) 941
BmgT120I GGNCC 2 cut(s) 155, 235
BmiI GGNNCC 7 cut(s) 236, 237, 468, 511, 699, 1476, 2268
BmrFI CCNGG 7 cut(s) 134, 641, 1341, 1444, 1995, 2608, 2763
BmsI GCATC 9 cut(s) 283, 1489, 1894, 2023, 2274, 2386, 2464, 2922, 2969
BmtI GCTAGC 1 cut(s) 1820
BpiI GAAGAC 2 cut(s) 1383, 2834
BpmI CTGGAG 5 cut(s) 148, 673, 1137, 2016, 2937
BpuMI CCSGG 1 cut(s) 1341
BsaBI GATNNNNATC 2 cut(s) 1509, 2757
BsaHI GRCGYC 2 cut(s) 626, 1249
BsaI GGTCTC 2 cut(s) 1227, 2349
BsaJI CCNNGG 7 cut(s) 133, 148, 181, 211, 640, 1708, 2607
BsaWI WCCGGW 1 cut(s) 2067
BsaXI ACNNNNNCTCC 2 cut(s) 2639, 2669
Bsc4I CCNNNNNNNGG 8 cut(s) 1026, 1174, 1449, 1745, 1746, 2276, 2644, 3043
Bse1I ACTGG 5 cut(s) 1074, 1120, 1162, 2528, 2920
Bse3DI GCAATG 3 cut(s) 792, 1827, 2043
Bse8I GATNNNNATC 2 cut(s) 1509, 2757
BseAI TCCGGA 1 cut(s) 2067
BseBI CCWGG 6 cut(s) 134, 641, 1444, 1995, 2608, 2763
BseDI CCNNGG 7 cut(s) 133, 148, 181, 211, 640, 1708, 2607
BseGI GGATG 6 cut(s) 537, 1504, 1669, 2471, 2758, 3052
BseJI GATNNNNATC 2 cut(s) 1509, 2757
BseLI CCNNNNNNNGG 8 cut(s) 1026, 1174, 1449, 1745, 1746, 2276, 2644, 3043
BseMI GCAATG 3 cut(s) 792, 1827, 2043
BseMII CTCAG 7 cut(s) 251, 822, 830, 926, 945, 989, 1710
BseNI ACTGG 5 cut(s) 1074, 1120, 1162, 2528, 2920
BsePI GCGCGC 1 cut(s) 25
BseRI GAGGAG 3 cut(s) 68, 228, 2237
BseXI GCAGC 6 cut(s) 156, 994, 2122, 2323, 2326, 2386
BseYI CCCAGC 5 cut(s) 683, 1659, 2672, 2777, 3037
BsgI GTGCAG 1 cut(s) 2714
Bsh1236I CGCG 2 cut(s) 27, 198
BshFI GGCC 6 cut(s) 141, 156, 210, 404, 639, 1983
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 2 cut(s) 1340, 2068
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 8 cut(s) 1026, 1174, 1449, 1745, 1746, 2276, 2644, 3043
BsmBI CGTCTC 1 cut(s) 1960
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 2 cut(s) 815, 2723
BsnI GGCC 6 cut(s) 141, 156, 210, 404, 639, 1983
Bso31I GGTCTC 2 cut(s) 1227, 2349
BsoBI CYCGRG 1 cut(s) 874
Bsp13I TCCGGA 1 cut(s) 2067
Bsp1407I TGTACA 2 cut(s) 2252, 3109
BspANI GGCC 6 cut(s) 141, 156, 210, 404, 639, 1983
BspCNI CTCAG 7 cut(s) 252, 823, 831, 925, 946, 988, 1711
BspEI TCCGGA 1 cut(s) 2067
BspFNI CGCG 2 cut(s) 27, 198
BspHI TCATGA 1 cut(s) 2029
BspLI GGNNCC 7 cut(s) 236, 237, 468, 511, 699, 1476, 2268
BspMAI CTGCAG 1 cut(s) 550
BspOI GCTAGC 1 cut(s) 1820
BspPI GGATC 9 cut(s) 1469, 1482, 1709, 2134, 2261, 2274, 2403, 2497, 2773
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 2 cut(s) 1227, 2349
BsrBI CCGCTC 4 cut(s) 68, 123, 251, 338
BsrDI GCAATG 3 cut(s) 792, 1827, 2043
BsrGI TGTACA 2 cut(s) 2252, 3109
BsrI ACTGG 5 cut(s) 1074, 1120, 1162, 2528, 2920
BssECI CCNNGG 7 cut(s) 133, 148, 181, 211, 640, 1708, 2607
BssHII GCGCGC 1 cut(s) 25
BssNI GRCGYC 2 cut(s) 626, 1249
BssT1I CCWWGG 1 cut(s) 1708
Bst2UI CCWGG 6 cut(s) 134, 641, 1444, 1995, 2608, 2763
Bst4CI ACNGT 6 cut(s) 661, 709, 1051, 1152, 1603, 1877
Bst6I CTCTTC 1 cut(s) 2701
BstACI GRCGYC 2 cut(s) 626, 1249
BstAFI CTTAAG 1 cut(s) 740
BstAUI TGTACA 2 cut(s) 2252, 3109
BstC8I GCNNGC 9 cut(s) 27, 178, 249, 578, 600, 1818, 1977, 1981, 2836
BstDEI CTNAG 8 cut(s) 260, 728, 831, 839, 912, 954, 975, 1719
BstDSI CCRYGG 1 cut(s) 181
BstEII GGTNACC 1 cut(s) 2942
BstENI CCTNNNNNAGG 1 cut(s) 1024
BstF5I GGATG 6 cut(s) 537, 1504, 1669, 2471, 2758, 3052
BstFNI CGCG 2 cut(s) 27, 198
BstH2I RGCGCY 3 cut(s) 177, 229, 1084
BstHHI GCGC 6 cut(s) 27, 29, 176, 228, 1083, 2997
BstNI CCWGG 6 cut(s) 134, 641, 1444, 1995, 2608, 2763
BstPI GGTNACC 1 cut(s) 2942
BstSCI CCNGG 7 cut(s) 132, 639, 1339, 1442, 1993, 2606, 2761
BstSFI CTRYAG 2 cut(s) 537, 546
BstUI CGCG 2 cut(s) 27, 198
BstV1I GCAGC 6 cut(s) 156, 994, 2122, 2323, 2326, 2386
BstV2I GAAGAC 2 cut(s) 1383, 2834
BstX2I RGATCY 6 cut(s) 1255, 1474, 1714, 2266, 2408, 2572
BstYI RGATCY 6 cut(s) 1255, 1474, 1714, 2266, 2408, 2572
BsuRI GGCC 6 cut(s) 141, 156, 210, 404, 639, 1983
BtgI CCRYGG 1 cut(s) 181
BtrI CACGTC 1 cut(s) 941
BtsCI GGATG 6 cut(s) 537, 1504, 1669, 2471, 2758, 3052
BtsI GCAGTG 1 cut(s) 2702
BtsIMutI CAGTG 3 cut(s) 627, 2437, 2702
Cac8I GCNNGC 9 cut(s) 27, 178, 249, 578, 600, 1818, 1977, 1981, 2836
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 2029
CfoI GCGC 6 cut(s) 27, 29, 176, 228, 1083, 2997
Cfr13I GGNCC 2 cut(s) 155, 235
CseI GACGC 1 cut(s) 615
Csp6I GTAC 2 cut(s) 2253, 3110
CviQI GTAC 2 cut(s) 2253, 3110
DdeI CTNAG 8 cut(s) 260, 728, 831, 839, 912, 954, 975, 1719
DraI TTTAAA 2 cut(s) 1911, 3018
EaeI YGGCCR 4 cut(s) 139, 402, 637, 1981
Eam1104I CTCTTC 1 cut(s) 2701
EarI CTCTTC 1 cut(s) 2701
EciI GGCGGA 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 1708
Eco31I GGTCTC 2 cut(s) 1227, 2349
Eco32I GATATC 1 cut(s) 2755
Eco47I GGWCC 1 cut(s) 235
Eco47III AGCGCT 2 cut(s) 227, 1082
Eco57I CTGAAG 2 cut(s) 603, 1938
Eco88I CYCGRG 1 cut(s) 874
Eco91I GGTNACC 1 cut(s) 2942
EcoNI CCTNNNNNAGG 1 cut(s) 1024
EcoO109I RGGNCCY 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 2942
EcoRII CCWGG 6 cut(s) 132, 639, 1442, 1993, 2606, 2761
EcoRV GATATC 1 cut(s) 2755
EcoT14I CCWWGG 1 cut(s) 1708
EcoT22I ATGCAT 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 1708
Esp3I CGTCTC 1 cut(s) 1960
FalI AAGNNNNNCTT 4 cut(s) 711, 743, 1463, 1495
FaqI GGGAC 1 cut(s) 248
FbaI TGATCA 2 cut(s) 379, 2434
FblI GTMKAC 1 cut(s) 536
FokI GGATG 6 cut(s) 544, 1511, 1676, 2478, 2745, 3059
FspBI CTAG 6 cut(s) 413, 923, 1373, 1553, 1817, 2345
GlaI GCGC 6 cut(s) 26, 28, 175, 227, 1082, 2996
GsaI CCCAGC 5 cut(s) 687, 1663, 2676, 2781, 3041
GsuI CTGGAG 5 cut(s) 148, 673, 1137, 2016, 2937
HaeII RGCGCY 3 cut(s) 177, 229, 1084
HaeIII GGCC 6 cut(s) 141, 156, 210, 404, 639, 1983
HapII CCGG 2 cut(s) 1340, 2068
HgaI GACGC 1 cut(s) 615
HhaI GCGC 6 cut(s) 27, 29, 176, 228, 1083, 2997
Hin1I GRCGYC 2 cut(s) 626, 1249
Hin6I GCGC 6 cut(s) 25, 27, 174, 226, 1081, 2995
HinP1I GCGC 6 cut(s) 25, 27, 174, 226, 1081, 2995
HincII GTYRAC 2 cut(s) 1155, 2599
HindII GTYRAC 2 cut(s) 1155, 2599
HindIII AAGCTT 5 cut(s) 600, 806, 1557, 2454, 3011
HpaI GTTAAC 1 cut(s) 1155
HpaII CCGG 2 cut(s) 1340, 2068
HphI GGTGA 4 cut(s) 890, 1055, 2432, 2517
Hpy166II GTNNAC 8 cut(s) 537, 1155, 1571, 2255, 2599, 2785, 3002, 3112
Hpy8I GTNNAC 8 cut(s) 537, 1155, 1571, 2255, 2599, 2785, 3002, 3112
Hpy99I CGWCG 1 cut(s) 1251
HpyAV CCTTC 8 cut(s) 439, 743, 757, 1247, 1443, 1465, 2456, 2957
HpyCH4III ACNGT 6 cut(s) 661, 709, 1051, 1152, 1603, 1877
HpyCH4IV ACGT 5 cut(s) 361, 940, 1249, 1349, 1380
HpyF3I CTNAG 8 cut(s) 260, 728, 831, 839, 912, 954, 975, 1719
HpySE526I ACGT 5 cut(s) 361, 940, 1249, 1349, 1380
Hsp92I GRCGYC 2 cut(s) 626, 1249
HspAI GCGC 6 cut(s) 25, 27, 174, 226, 1081, 2995
KflI GGGWCCC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 2067
Ksp22I TGATCA 2 cut(s) 379, 2434
KspAI GTTAAC 1 cut(s) 1155
Lsp1109I GCAGC 6 cut(s) 156, 994, 2122, 2323, 2326, 2386
LweI GCATC 9 cut(s) 283, 1489, 1894, 2023, 2274, 2386, 2464, 2922, 2969
MaeI CTAG 6 cut(s) 413, 923, 1373, 1553, 1817, 2345
MaeII ACGT 5 cut(s) 361, 940, 1249, 1349, 1380
MaeIII GTNAC 6 cut(s) 1526, 1677, 2003, 2052, 2530, 2942
MbiI CCGCTC 4 cut(s) 68, 123, 251, 338
MboII GAAGA 9 cut(s) 325, 938, 1232, 1388, 1505, 1727, 2718, 2839, 3037
MfeI CAATTG 1 cut(s) 2331
MflI RGATCY 6 cut(s) 1255, 1474, 1714, 2266, 2408, 2572
MlsI TGGCCA 1 cut(s) 639
MluCI AATT 9 cut(s) 364, 1224, 1438, 1689, 1809, 2061, 2331, 2661, 2990
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 6 cut(s) 69, 571, 755, 1424, 1457, 2846
MmeI TCCRAC 5 cut(s) 150, 1443, 2014, 2104, 2930
Mox20I TGGCCA 1 cut(s) 639
Mph1103I ATGCAT 1 cut(s) 433
MroI TCCGGA 1 cut(s) 2067
MroXI GAANNNNTTC 1 cut(s) 807
MscI TGGCCA 1 cut(s) 639
MslI CAYNNNNRTG 2 cut(s) 426, 2930
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 2 cut(s) 1340, 2068
MspR9I CCNGG 7 cut(s) 134, 641, 1341, 1444, 1995, 2608, 2763
MunI CAATTG 1 cut(s) 2331
Mva1269I GAATGC 2 cut(s) 815, 2723
MvaI CCWGG 6 cut(s) 134, 641, 1444, 1995, 2608, 2763
MvnI CGCG 2 cut(s) 27, 198
NciI CCSGG 1 cut(s) 1341
NheI GCTAGC 1 cut(s) 1816
NlaIV GGNNCC 7 cut(s) 236, 237, 468, 511, 699, 1476, 2268
NmeAIII GCCGAG 3 cut(s) 173, 236, 301
NsiI ATGCAT 1 cut(s) 433
PagI TCATGA 1 cut(s) 2029
PauI GCGCGC 1 cut(s) 25
PctI GAATGC 2 cut(s) 815, 2723
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 9 cut(s) 526, 1036, 1063, 1073, 1704, 2026, 2159, 2423, 2983
PflMI CCANNNNNTGG 3 cut(s) 1174, 2276, 2644
PfoI TCCNGGA 2 cut(s) 1442, 2761
PleI GAGTC 6 cut(s) 69, 570, 754, 1423, 1457, 2846
PpsI GAGTC 6 cut(s) 69, 570, 754, 1423, 1457, 2846
PpuMI RGGWCCY 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 1764
Psp1406I AACGTT 1 cut(s) 1349
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 6 cut(s) 132, 639, 1442, 1993, 2606, 2761
PspEI GGTNACC 1 cut(s) 2942
PspFI CCCAGC 5 cut(s) 683, 1659, 2672, 2777, 3037
PspGI CCWGG 6 cut(s) 132, 639, 1442, 1993, 2606, 2761
PspN4I GGNNCC 7 cut(s) 236, 237, 468, 511, 699, 1476, 2268
PspPI GGNCC 2 cut(s) 155, 235
PspPPI RGGWCCY 1 cut(s) 235
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 6 cut(s) 1255, 1474, 1714, 2266, 2408, 2572
PteI GCGCGC 1 cut(s) 25
RsaI GTAC 2 cut(s) 2254, 3111
RsaNI GTAC 2 cut(s) 2253, 3110
RseI CAYNNNNRTG 2 cut(s) 426, 2930
Sau96I GGNCC 2 cut(s) 155, 235
SchI GAGTC 6 cut(s) 69, 571, 755, 1424, 1457, 2846
ScrFI CCNGG 7 cut(s) 134, 641, 1341, 1444, 1995, 2608, 2763
SfaNI GCATC 9 cut(s) 283, 1489, 1894, 2023, 2274, 2386, 2464, 2922, 2969
SfcI CTRYAG 2 cut(s) 537, 546
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 426, 2930
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 9 cut(s) 364, 1224, 1438, 1689, 1809, 2061, 2331, 2661, 2990
SspI AATATT 1 cut(s) 1779
SspMI CTAG 6 cut(s) 413, 923, 1373, 1553, 1817, 2345
StyD4I CCNGG 7 cut(s) 132, 639, 1339, 1442, 1993, 2606, 2761
StyI CCWWGG 1 cut(s) 1708
TaaI ACNGT 6 cut(s) 661, 709, 1051, 1152, 1603, 1877
TaiI ACGT 5 cut(s) 364, 943, 1252, 1352, 1383
TaqI TCGA 6 cut(s) 1066, 1246, 2184, 2353, 2582, 2751
TasI AATT 9 cut(s) 364, 1224, 1438, 1689, 1809, 2061, 2331, 2661, 2990
TatI WGTACW 2 cut(s) 2252, 3109
TauI GCSGC 9 cut(s) 68, 123, 144, 156, 171, 174, 201, 204, 275
TfiI GAWTC 9 cut(s) 526, 1036, 1063, 1073, 1704, 2026, 2159, 2423, 2983
TscAI CASTG 3 cut(s) 627, 2437, 2702
TseI GCWGC 6 cut(s) 144, 1007, 2110, 2336, 2339, 2399
TspRI CASTG 3 cut(s) 627, 2437, 2702
Van91I CCANNNNNTGG 3 cut(s) 1174, 2276, 2644
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 235
VspI ATTAAT 1 cut(s) 1764
XagI CCTNNNNNAGG 1 cut(s) 1024
XapI RAATTY 2 cut(s) 1438, 2061
XbaI TCTAGA 3 cut(s) 922, 1372, 1552
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 6 cut(s) 413, 923, 1373, 1553, 1817, 2345
ZraI GACGTC 1 cut(s) 1250
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.