Rh3DG230200

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007834
Physical Location & Seq
Reverse (-)
21878217 .. 21886852
8636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG230200.1

Sequence Viewer

Length: 3147 bp
ATGTGTTGTTTGCTCTTAACTTTGCGCGCAACCACCAACCACTTTCTCCCCACTCACTTCCTCGCCGCTCCACGACTCCTCACTTTTCGCTCTGCCATCTCAACTCTCGCCATGTCCAGCCGCTCCAACTTCCAGGGCGGCCGCAGCAGAGGCGGCCCTAACTCCCGCGGCGGCGCTCGCCGTGGAGGCGGCGCTCGCGGCGGTGGAGGCCGAGGAGGAGGACGCGGCGGCGGCGGCGGCCGAGGCGGCGAACAGCGCTGGTGGGACCCCGTGTGGCGAGCCGAGCGACTGAGGCAACAAGCCGCCGAGATGGAAGTTCTTGATGCGAGTGAGTATTGGGGGAAGATGGAGCAGTTCAGAAGCGGAGCGGAGCAGGAGATGATAATAAGACGTAATTTTAGTCGGCATGATCAGCAAACCCTATATGAAATGGCCGATGAACTAGGGCTTCACTTCCATGCATATAATAAGGGGAAGGCTCTTGTGGTCAGCAAGGTTCCTCTGCCAGATTATCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGGATGTCTACAGAGACTGCAGAGAGAGTCGGGAGTCTTTTGCAAAGCTCGCAGGGGCAGGGAAGTAAGCAAGCTTTTGGTAATGTTGCTTCAGTGGCGTCTGGGTCTGGCCAGGGGAACAAACTAACAACTATTAGTGAAAATACAACCAAACCCAGCTCCAGCTTGGAGCTTGATACTGTTAAGGAGAAAGAGAAACTTAGCCTTCAACTTAAGGAGTCGCAGGAAAAAATGAAGGTAAGTAATAGTCTGAAAGCAATGCTGACATTCCGAGAAAAGCTTCCTGCATTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGAGTGCAACATAATATGCACTCAGCCTCGCCGTATATCTGCCGTGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACAACTGGAGTATTACTTCGTCAGTTGGTTCAAGACCCACAGTTAACTGGCGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAACGAGGACTTTTTACTAATAATTTTGCGAGACCTTCTTCCTCGACGTCCAGATCTACGACTTATTCTAATGAGTGCTACCATCAATGCTGACTTGTTTTCCAAATACTTTGGAAATGCCCCAACAATACATATACCGGGATTAACGTTCCCTGTCGCAGAGCTATTTCTAGAAGACGTTTTGGAGAAAACTCATTATATAGTCAAGTCAGAGTCCGACAAGATGGAGAGGGGAAATTCCAGGAGAAGGAGACAGCAAGACTCCAAGAAGGATCCCTTAACTGAACTATTTGAGGATGTTGATATTGATCTTCACTACAAAAGTTACAGCACATCTACAAGAAAGTCTCTAGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTCTGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACAGGCTGGGATGATATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCTGAGAAGTTCATGGTTCTTCCCCTTCATGGGTCAATGCCTACTATCAATCAACGAGAAATATTTGATCGTCCTCCCACTACCAAGAGAAAAATTGTGCTAGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGTGGAAAGGCAAAGGAAACGAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATACCCAAAAATGATTCACGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTTTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCCTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGAGCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTCAACAAATCCAGGGGTGCTAACGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTTGGTAAAGTCGATATCCATCCTGGATCAGTCAATGCTGGGGTTCACTTATTCCCTCTGCCTTACATGGTATATAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTTGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTAGTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1048

Amino Acids

116.35

Weight (kDa)

8.84

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 287 - 441 3.1e-09 DEAD/DEAH box helicase
Helicase_C PF00271 546 - 673 2.1e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 737 - 765 1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 766 - 825 2.5e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 896 - 976 1e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 986 - 1043 1.4e-21 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1282
AccB7I CCANNNNNTGG 3 cut(s) 1204, 2306, 2674
AccBSI CCGCTC 3 cut(s) 68, 123, 368
AccI GTMKAC 1 cut(s) 566
AccII CGCG 4 cut(s) 27, 168, 198, 225
AccIII TCCGGA 1 cut(s) 2097
AclI AACGTT 1 cut(s) 1379
AclWI GGATC 8 cut(s) 1499, 1512, 1739, 2164, 2291, 2304, 2527, 2803
AcoI YGGCCR 5 cut(s) 139, 238, 432, 667, 2011
AcsI RAATTY 2 cut(s) 1468, 2091
AcuI CTGAAG 2 cut(s) 633, 1968
AcyI GRCGYC 2 cut(s) 656, 1279
AfaI GTAC 2 cut(s) 2284, 3141
AfeI AGCGCT 2 cut(s) 257, 1112
AfiI CCNNNNNNNGG 8 cut(s) 1056, 1204, 1479, 1775, 1776, 2306, 2674, 3073
AflII CTTAAG 1 cut(s) 770
AgsI TTSAA 8 cut(s) 767, 850, 1172, 1977, 2180, 2204, 2339, 2492
AjiI CACGTC 1 cut(s) 971
AjnI CCWGG 6 cut(s) 132, 669, 1472, 2023, 2636, 2791
AlwI GGATC 8 cut(s) 1499, 1512, 1739, 2164, 2291, 2304, 2527, 2803
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 904
Aor13HI TCCGGA 1 cut(s) 2097
Aor51HI AGCGCT 2 cut(s) 257, 1112
AoxI GGCC 7 cut(s) 139, 154, 208, 238, 432, 667, 2011
ApeKI GCWGC 6 cut(s) 144, 1037, 2140, 2366, 2369, 2429
ApoI RAATTY 2 cut(s) 1468, 2091
Asp700I GAANNNNTTC 1 cut(s) 837
AspLEI GCGC 7 cut(s) 27, 29, 176, 194, 258, 1113, 3027
AspS9I GGNCC 2 cut(s) 155, 265
AsuC2I CCSGG 1 cut(s) 1371
AsuHPI GGTGA 4 cut(s) 920, 1085, 2462, 2547
AsuNHI GCTAGC 1 cut(s) 1846
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 1 cut(s) 265
BalI TGGCCA 1 cut(s) 669
BamHI GGATCC 2 cut(s) 1504, 2296
BarI GAAGNNNNNNTAC 2 cut(s) 631, 663
BbsI GAAGAC 2 cut(s) 1413, 2864
BbvI GCAGC 6 cut(s) 156, 1024, 2152, 2353, 2356, 2416
BceAI ACGGC 5 cut(s) 165, 999, 1010, 1998, 3012
BciT130I CCWGG 6 cut(s) 134, 671, 1474, 2025, 2638, 2793
BclI TGATCA 2 cut(s) 409, 2464
BcnI CCSGG 1 cut(s) 1371
BfaI CTAG 7 cut(s) 443, 953, 1403, 1583, 1847, 2375, 3084
BfmI CTRYAG 2 cut(s) 567, 576
BfoI RGCGCY 4 cut(s) 177, 195, 259, 1114
BfrI CTTAAG 1 cut(s) 770
BglI GCCNNNNNGGC 2 cut(s) 186, 246
BglII AGATCT 2 cut(s) 1285, 2602
Bme1390I CCNGG 7 cut(s) 134, 671, 1371, 1474, 2025, 2638, 2793
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 904
BmgBI CACGTC 1 cut(s) 971
BmgT120I GGNCC 2 cut(s) 155, 265
BmiI GGNNCC 6 cut(s) 266, 267, 498, 541, 1506, 2298
BmrFI CCNGG 7 cut(s) 134, 671, 1371, 1474, 2025, 2638, 2793
BmsI GCATC 8 cut(s) 313, 1924, 2053, 2304, 2416, 2494, 2952, 2999
BmtI GCTAGC 1 cut(s) 1850
BpiI GAAGAC 2 cut(s) 1413, 2864
BpmI CTGGAG 4 cut(s) 703, 1167, 2046, 2967
BpuMI CCSGG 1 cut(s) 1371
BsaBI GATNNNNATC 2 cut(s) 1539, 2787
BsaHI GRCGYC 2 cut(s) 656, 1279
BsaI GGTCTC 2 cut(s) 1257, 2379
BsaJI CCNNGG 8 cut(s) 133, 166, 181, 211, 241, 670, 1738, 2637
BsaWI WCCGGW 1 cut(s) 2097
BsaXI ACNNNNNCTCC 2 cut(s) 2669, 2699
Bsc4I CCNNNNNNNGG 8 cut(s) 1056, 1204, 1479, 1775, 1776, 2306, 2674, 3073
Bse1I ACTGG 5 cut(s) 1104, 1150, 1192, 2558, 2950
Bse3DI GCAATG 3 cut(s) 822, 1857, 2073
Bse8I GATNNNNATC 2 cut(s) 1539, 2787
BseAI TCCGGA 1 cut(s) 2097
BseBI CCWGG 6 cut(s) 134, 671, 1474, 2025, 2638, 2793
BseDI CCNNGG 8 cut(s) 133, 166, 181, 211, 241, 670, 1738, 2637
BseGI GGATG 6 cut(s) 567, 1534, 1699, 2501, 2788, 3082
BseJI GATNNNNATC 2 cut(s) 1539, 2787
BseLI CCNNNNNNNGG 8 cut(s) 1056, 1204, 1479, 1775, 1776, 2306, 2674, 3073
BseMI GCAATG 3 cut(s) 822, 1857, 2073
BseMII CTCAG 7 cut(s) 281, 852, 860, 956, 975, 1019, 1740
BseNI ACTGG 5 cut(s) 1104, 1150, 1192, 2558, 2950
BsePI GCGCGC 1 cut(s) 25
BseRI GAGGAG 4 cut(s) 68, 228, 231, 2267
BseX3I CGGCCG 2 cut(s) 139, 238
BseXI GCAGC 6 cut(s) 156, 1024, 2152, 2353, 2356, 2416
BseYI CCCAGC 5 cut(s) 713, 1689, 2702, 2807, 3067
BsgI GTGCAG 1 cut(s) 2744
Bsh1236I CGCG 4 cut(s) 27, 168, 198, 225
Bsh1285I CGRYCG 2 cut(s) 142, 241
BshFI GGCC 7 cut(s) 141, 156, 210, 240, 434, 669, 2013
BsiEI CGRYCG 2 cut(s) 142, 241
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 2 cut(s) 1370, 2098
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 8 cut(s) 1056, 1204, 1479, 1775, 1776, 2306, 2674, 3073
BsmBI CGTCTC 1 cut(s) 1990
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 2 cut(s) 845, 2753
BsnI GGCC 7 cut(s) 141, 156, 210, 240, 434, 669, 2013
Bso31I GGTCTC 2 cut(s) 1257, 2379
BsoBI CYCGRG 1 cut(s) 904
Bsp13I TCCGGA 1 cut(s) 2097
Bsp1407I TGTACA 2 cut(s) 2282, 3139
BspANI GGCC 7 cut(s) 141, 156, 210, 240, 434, 669, 2013
BspCNI CTCAG 7 cut(s) 282, 853, 861, 955, 976, 1018, 1741
BspEI TCCGGA 1 cut(s) 2097
BspFNI CGCG 4 cut(s) 27, 168, 198, 225
BspLI GGNNCC 6 cut(s) 266, 267, 498, 541, 1506, 2298
BspMAI CTGCAG 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 1850
BspPI GGATC 8 cut(s) 1499, 1512, 1739, 2164, 2291, 2304, 2527, 2803
BspTI CTTAAG 1 cut(s) 770
BspTNI GGTCTC 2 cut(s) 1257, 2379
BsrBI CCGCTC 3 cut(s) 68, 123, 368
BsrDI GCAATG 3 cut(s) 822, 1857, 2073
BsrGI TGTACA 2 cut(s) 2282, 3139
BsrI ACTGG 5 cut(s) 1104, 1150, 1192, 2558, 2950
BssECI CCNNGG 8 cut(s) 133, 166, 181, 211, 241, 670, 1738, 2637
BssHII GCGCGC 1 cut(s) 25
BssNI GRCGYC 2 cut(s) 656, 1279
BssT1I CCWWGG 1 cut(s) 1738
Bst2UI CCWGG 6 cut(s) 134, 671, 1474, 2025, 2638, 2793
Bst4CI ACNGT 5 cut(s) 739, 1081, 1182, 1633, 1907
Bst6I CTCTTC 1 cut(s) 2731
BstACI GRCGYC 2 cut(s) 656, 1279
BstAFI CTTAAG 1 cut(s) 770
BstAUI TGTACA 2 cut(s) 2282, 3139
BstDEI CTNAG 8 cut(s) 290, 758, 861, 869, 942, 984, 1005, 1749
BstDSI CCRYGG 2 cut(s) 166, 181
BstEII GGTNACC 1 cut(s) 2972
BstENI CCTNNNNNAGG 1 cut(s) 1054
BstF5I GGATG 6 cut(s) 567, 1534, 1699, 2501, 2788, 3082
BstFNI CGCG 4 cut(s) 27, 168, 198, 225
BstH2I RGCGCY 4 cut(s) 177, 195, 259, 1114
BstHHI GCGC 7 cut(s) 27, 29, 176, 194, 258, 1113, 3027
BstMCI CGRYCG 2 cut(s) 142, 241
BstNI CCWGG 6 cut(s) 134, 671, 1474, 2025, 2638, 2793
BstPI GGTNACC 1 cut(s) 2972
BstSCI CCNGG 7 cut(s) 132, 669, 1369, 1472, 2023, 2636, 2791
BstSFI CTRYAG 2 cut(s) 567, 576
BstUI CGCG 4 cut(s) 27, 168, 198, 225
BstV1I GCAGC 6 cut(s) 156, 1024, 2152, 2353, 2356, 2416
BstV2I GAAGAC 2 cut(s) 1413, 2864
BstX2I RGATCY 5 cut(s) 1285, 1504, 1744, 2296, 2602
BstYI RGATCY 5 cut(s) 1285, 1504, 1744, 2296, 2602
BstZI CGGCCG 2 cut(s) 139, 238
BsuRI GGCC 7 cut(s) 141, 156, 210, 240, 434, 669, 2013
BtgI CCRYGG 2 cut(s) 166, 181
BtrI CACGTC 1 cut(s) 971
BtsCI GGATG 6 cut(s) 567, 1534, 1699, 2501, 2788, 3082
BtsI GCAGTG 1 cut(s) 2732
BtsIMutI CAGTG 3 cut(s) 657, 2467, 2732
CaiI CAGNNNCTG 1 cut(s) 575
CciNI GCGGCCGC 1 cut(s) 139
CfoI GCGC 7 cut(s) 27, 29, 176, 194, 258, 1113, 3027
Cfr13I GGNCC 2 cut(s) 155, 265
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 2 cut(s) 231, 645
Csp6I GTAC 2 cut(s) 2283, 3140
CviQI GTAC 2 cut(s) 2283, 3140
DdeI CTNAG 8 cut(s) 290, 758, 861, 869, 942, 984, 1005, 1749
DraI TTTAAA 2 cut(s) 1941, 3048
EaeI YGGCCR 5 cut(s) 139, 238, 432, 667, 2011
EagI CGGCCG 2 cut(s) 139, 238
Eam1104I CTCTTC 1 cut(s) 2731
EarI CTCTTC 1 cut(s) 2731
EclXI CGGCCG 2 cut(s) 139, 238
Eco130I CCWWGG 1 cut(s) 1738
Eco31I GGTCTC 2 cut(s) 1257, 2379
Eco32I GATATC 1 cut(s) 2785
Eco47I GGWCC 1 cut(s) 265
Eco47III AGCGCT 2 cut(s) 257, 1112
Eco52I CGGCCG 2 cut(s) 139, 238
Eco57I CTGAAG 2 cut(s) 633, 1968
Eco88I CYCGRG 1 cut(s) 904
Eco91I GGTNACC 1 cut(s) 2972
EcoNI CCTNNNNNAGG 1 cut(s) 1054
EcoO109I RGGNCCY 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 2972
EcoRII CCWGG 6 cut(s) 132, 669, 1472, 2023, 2636, 2791
EcoRV GATATC 1 cut(s) 2785
EcoT14I CCWWGG 1 cut(s) 1738
EcoT22I ATGCAT 1 cut(s) 463
ErhI CCWWGG 1 cut(s) 1738
Esp3I CGTCTC 1 cut(s) 1990
FalI AAGNNNNNCTT 4 cut(s) 741, 773, 1493, 1525
FaqI GGGAC 1 cut(s) 278
FauI CCCGC 1 cut(s) 173
FbaI TGATCA 2 cut(s) 409, 2464
FblI GTMKAC 1 cut(s) 566
FokI GGATG 6 cut(s) 574, 1541, 1706, 2508, 2775, 3089
FspBI CTAG 7 cut(s) 443, 953, 1403, 1583, 1847, 2375, 3084
GlaI GCGC 7 cut(s) 26, 28, 175, 193, 257, 1112, 3026
GsaI CCCAGC 5 cut(s) 717, 1693, 2706, 2811, 3071
GsuI CTGGAG 4 cut(s) 703, 1167, 2046, 2967
HaeII RGCGCY 4 cut(s) 177, 195, 259, 1114
HaeIII GGCC 7 cut(s) 141, 156, 210, 240, 434, 669, 2013
HapII CCGG 2 cut(s) 1370, 2098
HgaI GACGC 2 cut(s) 231, 645
HhaI GCGC 7 cut(s) 27, 29, 176, 194, 258, 1113, 3027
Hin1I GRCGYC 2 cut(s) 656, 1279
Hin6I GCGC 7 cut(s) 25, 27, 174, 192, 256, 1111, 3025
HinP1I GCGC 7 cut(s) 25, 27, 174, 192, 256, 1111, 3025
HincII GTYRAC 2 cut(s) 1185, 2629
HindII GTYRAC 2 cut(s) 1185, 2629
HindIII AAGCTT 5 cut(s) 630, 836, 1587, 2484, 3041
HpaI GTTAAC 1 cut(s) 1185
HpaII CCGG 2 cut(s) 1370, 2098
HphI GGTGA 4 cut(s) 920, 1085, 2462, 2547
Hpy166II GTNNAC 8 cut(s) 567, 1185, 1601, 2285, 2629, 2815, 3032, 3142
Hpy8I GTNNAC 8 cut(s) 567, 1185, 1601, 2285, 2629, 2815, 3032, 3142
Hpy99I CGWCG 1 cut(s) 1281
HpyAV CCTTC 9 cut(s) 469, 773, 787, 1277, 1473, 1495, 1781, 2486, 2987
HpyCH4III ACNGT 5 cut(s) 739, 1081, 1182, 1633, 1907
HpyCH4IV ACGT 5 cut(s) 391, 970, 1279, 1379, 1410
HpyF3I CTNAG 8 cut(s) 290, 758, 861, 869, 942, 984, 1005, 1749
HpySE526I ACGT 5 cut(s) 391, 970, 1279, 1379, 1410
Hsp92I GRCGYC 2 cut(s) 656, 1279
HspAI GCGC 7 cut(s) 25, 27, 174, 192, 256, 1111, 3025
KflI GGGWCCC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 2097
Ksp22I TGATCA 2 cut(s) 409, 2464
KspAI GTTAAC 1 cut(s) 1185
KspI CCGCGG 1 cut(s) 169
Lsp1109I GCAGC 6 cut(s) 156, 1024, 2152, 2353, 2356, 2416
LweI GCATC 8 cut(s) 313, 1924, 2053, 2304, 2416, 2494, 2952, 2999
MaeI CTAG 7 cut(s) 443, 953, 1403, 1583, 1847, 2375, 3084
MaeII ACGT 5 cut(s) 391, 970, 1279, 1379, 1410
MaeIII GTNAC 6 cut(s) 1556, 1707, 2033, 2082, 2560, 2972
MbiI CCGCTC 3 cut(s) 68, 123, 368
MboII GAAGA 9 cut(s) 355, 968, 1262, 1418, 1535, 1757, 2748, 2869, 3067
MfeI CAATTG 1 cut(s) 2361
MflI RGATCY 5 cut(s) 1285, 1504, 1744, 2296, 2602
MlsI TGGCCA 1 cut(s) 669
MluCI AATT 9 cut(s) 394, 1254, 1468, 1719, 1839, 2091, 2361, 2691, 3020
MluNI TGGCCA 1 cut(s) 669
MlyI GAGTC 7 cut(s) 69, 594, 601, 785, 1454, 1487, 2876
MmeI TCCRAC 5 cut(s) 150, 1473, 2044, 2134, 2960
Mox20I TGGCCA 1 cut(s) 669
Mph1103I ATGCAT 1 cut(s) 463
MroI TCCGGA 1 cut(s) 2097
MroXI GAANNNNTTC 1 cut(s) 837
MscI TGGCCA 1 cut(s) 669
MslI CAYNNNNRTG 2 cut(s) 456, 2960
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 2 cut(s) 1370, 2098
MspR9I CCNGG 7 cut(s) 134, 671, 1371, 1474, 2025, 2638, 2793
MunI CAATTG 1 cut(s) 2361
Mva1269I GAATGC 2 cut(s) 845, 2753
MvaI CCWGG 6 cut(s) 134, 671, 1474, 2025, 2638, 2793
MvnI CGCG 4 cut(s) 27, 168, 198, 225
NciI CCSGG 1 cut(s) 1371
NheI GCTAGC 1 cut(s) 1846
NlaIV GGNNCC 6 cut(s) 266, 267, 498, 541, 1506, 2298
NmeAIII GCCGAG 4 cut(s) 236, 266, 307, 331
NotI GCGGCCGC 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 463
PauI GCGCGC 1 cut(s) 25
PctI GAATGC 2 cut(s) 845, 2753
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 9 cut(s) 556, 1066, 1093, 1103, 1734, 2056, 2189, 2453, 3013
PflMI CCANNNNNTGG 3 cut(s) 1204, 2306, 2674
PfoI TCCNGGA 2 cut(s) 1472, 2791
PleI GAGTC 7 cut(s) 69, 593, 600, 784, 1453, 1487, 2876
PpsI GAGTC 7 cut(s) 69, 593, 600, 784, 1453, 1487, 2876
PpuMI RGGWCCY 1 cut(s) 265
Psp1406I AACGTT 1 cut(s) 1379
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 6 cut(s) 132, 669, 1472, 2023, 2636, 2791
PspEI GGTNACC 1 cut(s) 2972
PspFI CCCAGC 5 cut(s) 713, 1689, 2702, 2807, 3067
PspGI CCWGG 6 cut(s) 132, 669, 1472, 2023, 2636, 2791
PspN4I GGNNCC 6 cut(s) 266, 267, 498, 541, 1506, 2298
PspPI GGNCC 2 cut(s) 155, 265
PspPPI RGGWCCY 1 cut(s) 265
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 5 cut(s) 1285, 1504, 1744, 2296, 2602
PteI GCGCGC 1 cut(s) 25
RsaI GTAC 2 cut(s) 2284, 3141
RsaNI GTAC 2 cut(s) 2283, 3140
RseI CAYNNNNRTG 2 cut(s) 456, 2960
SacII CCGCGG 1 cut(s) 169
Sau96I GGNCC 2 cut(s) 155, 265
SchI GAGTC 7 cut(s) 69, 594, 601, 785, 1454, 1487, 2876
ScrFI CCNGG 7 cut(s) 134, 671, 1371, 1474, 2025, 2638, 2793
SfaNI GCATC 8 cut(s) 313, 1924, 2053, 2304, 2416, 2494, 2952, 2999
SfcI CTRYAG 2 cut(s) 567, 576
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 456, 2960
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 9 cut(s) 394, 1254, 1468, 1719, 1839, 2091, 2361, 2691, 3020
SspI AATATT 1 cut(s) 1809
SspMI CTAG 7 cut(s) 443, 953, 1403, 1583, 1847, 2375, 3084
StyD4I CCNGG 7 cut(s) 132, 669, 1369, 1472, 2023, 2636, 2791
StyI CCWWGG 1 cut(s) 1738
TaaI ACNGT 5 cut(s) 739, 1081, 1182, 1633, 1907
TaiI ACGT 5 cut(s) 394, 973, 1282, 1382, 1413
TaqI TCGA 6 cut(s) 1096, 1276, 2214, 2383, 2612, 2781
TasI AATT 9 cut(s) 394, 1254, 1468, 1719, 1839, 2091, 2361, 2691, 3020
TatI WGTACW 2 cut(s) 2282, 3139
TfiI GAWTC 9 cut(s) 556, 1066, 1093, 1103, 1734, 2056, 2189, 2453, 3013
TscAI CASTG 3 cut(s) 657, 2467, 2732
TseI GCWGC 6 cut(s) 144, 1037, 2140, 2366, 2369, 2429
TspRI CASTG 3 cut(s) 657, 2467, 2732
Van91I CCANNNNNTGG 3 cut(s) 1204, 2306, 2674
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 1054
XapI RAATTY 2 cut(s) 1468, 2091
XbaI TCTAGA 3 cut(s) 952, 1402, 1582
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 7 cut(s) 443, 953, 1403, 1583, 1847, 2375, 3084
ZraI GACGTC 1 cut(s) 1280
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.