Rroxscaffold_6G00405420

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007834
Physical Location & Seq
Forward (+)
27794151 .. 27803537
9387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405420.1

Sequence Viewer

Length: 3264 bp
ATGTCCAGCCGCCCGAACTTCCAGGGTGGCCGCAGCAGAGGCGGCCCTAACTCCCGCGGCGGCGCTCGCCGTGGAGGCGGTGGTCGCGGCGGTGGAGGCCGAGGAGGAGGAGGACGCGGCGGCGGCCGAGGCGGCGAACAGCGCTGGTGGGACCCCGTGTGGCGAGCGGAGCGACTGAGGCAACAAGCCGCCGAGATGGAAGTTCTCGATGCGAGTGAGTATTGGGGGAGGATGGAGCAGTTCAGAAGCGGAGCGGAGCAGGAGATGATAATACGACGTAATTTTAGTCGGCATGATCAGCAAACCCTATATGAAATGGCCTATGAACTAGGGCTTCACTTCCATGCATATAATAAGGGGAAGGCTCTTGTGGTCAGCAAGGTTCCTCTGCCAGATTATCGGGCAGACCTTGATGATGTTCATGGTTCCACACAAAAAGAGATTCGAATGTCTACAGAGACTGCAGAGAGAGTCGGGAGTCTTTTGCAAAGCTCTCAGGGGCAGGGAAGTAAGCAAGCTTTTGGTAATGTTGTTTCAGTGGCGTCTGGGTCTGGCCAGGGGAACAAACTAACAACTGTTAGTGAAAATACAACCAAACCCAGCTCCCGCTTGGAGCCTGATACTGTTAAGGAGAAAGAGAAACTTAGCCTTCAACTTAAGGAGTCGCAGGAAAAAATGAAGGTAAGTAATAGTCTGAAAGCAATGCTGACATTCCGAGAAAAGCTTCCTGCATTCAATGTGAAGTCTGAGTTCCTGAGAGCTGTTTCTGAAAATCAGGTGTTGGTAGTCTCGGGTGAGACAGGTTGTGGCAAAACAACACAACTTCCTCAGTTTATTCTAGAAGATGAGATTTCACGTCTGCATGGTGCTGAGTGCAACATAATATGCACTCAGCCTCGCCGTATATCTGCCGTGTCTGTTGCTGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATTCGACTGGAATCAAAGCGCTCTGCTCAAACAAGATTGCTATTTTGCACAACTGGAGTATTACTTCGTCAGTTGCGTATTCTAGTTAAGAGTGCTACCTTGATTGCTAAACTGCCCCATTTTCCCATTTCTTATGCTTATCCTTCGTATCCTTTAGCTTGGGATTTGGCATTCCAGAGGAAGCTAGGGTTGAGGAGGTTGATCACTGGTGGGAAAAAGTTCAATACTCGGCTTTGTTTGGGCATCAGTTTCTTCAAAGTTTTAAATAGCTCTTTTCAGGCTTTCTTTTTAGATGAGAGTGTTGTTGTGGTTCAAGACCCACAGTTAACTGGCGTGAGCCATTTGTTGGTTGATGAAATCCATGAAAGAGGCATGAACGAGGACTTTTTACTAATAATTTTGCGAGACCTTCTTCCTCGACGTCCAGATCTACGACTTATTCTAATGAGTGCTACCATCAATGCTGACTTGTTTTCCAAATACTTTGGAAATGCCCCAACAATACATATACCGGGATTAACGTTCCCTGTGGCAGAGCTATTTCTAGAAGACGTTTTGGAGAAAACTCATTATATAGTCAAGTCAGAGTCCGACAAGATGGAGAGGGGAAATTCCAGGAGAAGGAGACAGCAAGACTCCAAGAAGGATCCCTTAACTGAACTATTTGAGGCAATCTTACTTTCCTATCATTCTAAATTTCTTACCAAATCAATTCTAAAACTTGATGTTGATATTGATCTTCACTACAAAAGTTACAGCACATCTACAAGAAAGTCTCTAGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTCTGGTGGAGGCAACCGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACTGGCTGGGATGATATATCAAAGTTACTTGACAAAATTAAGGGAAACAGATTCCTTGGAGATCCTGAGAAGTTCATGGTTCTTCCCCTGCATGGAAAAATTGTGCTGGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGTGGAAAGGCAAAGGAAACGAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATACCCAAAAATGATTCATGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTTTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCCTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGATCCTTTGCTGGTGATTCTTTCAGCGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTCGACAAATCCAGGGGTGCTAATGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTACACCAAAGAAGTTGGTAAAGTTGATATCCATCCTGGATCAGTCAATGCTGGCGTTCATTTATTCCCTCTGCCCTACATGGTATACAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCACCAACGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTTGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTAGTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1087

Amino Acids

120.98

Weight (kDa)

9.06

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_HA2 PF04408 776 - 804 1.1e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 805 - 864 2.5e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 935 - 1015 1.1e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1025 - 1082 1.5e-21 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1402
AccB7I CCANNNNNTGG 3 cut(s) 1324, 2423, 2791
AccBSI CCGCTC 2 cut(s) 167, 254
AccI GTMKAC 3 cut(s) 452, 2745, 2958
AccII CGCG 3 cut(s) 57, 87, 117
AccIII TCCGGA 1 cut(s) 2214
AclI AACGTT 1 cut(s) 1499
AclWI GGATC 9 cut(s) 1619, 1632, 1913, 2281, 2408, 2421, 2550, 2644, 2920
AcoI YGGCCR 4 cut(s) 28, 124, 553, 2128
AcsI RAATTY 3 cut(s) 1588, 1673, 2208
AcuI CTGAAG 1 cut(s) 2085
AcyI GRCGYC 2 cut(s) 542, 1399
AfaI GTAC 2 cut(s) 2401, 3258
AfeI AGCGCT 2 cut(s) 143, 998
AfiI CCNNNNNNNGG 7 cut(s) 942, 1324, 1599, 1949, 2423, 2791, 3190
AflII CTTAAG 1 cut(s) 656
AjiI CACGTC 1 cut(s) 857
AjnI CCWGG 6 cut(s) 21, 555, 1592, 2140, 2753, 2908
AlwI GGATC 9 cut(s) 1619, 1632, 1913, 2281, 2408, 2421, 2550, 2644, 2920
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 790
Aor13HI TCCGGA 1 cut(s) 2214
Aor51HI AGCGCT 2 cut(s) 143, 998
AoxI GGCC 7 cut(s) 28, 43, 97, 124, 318, 553, 2128
ApeKI GCWGC 6 cut(s) 33, 923, 2257, 2483, 2486, 2546
ApoI RAATTY 3 cut(s) 1588, 1673, 2208
Asp700I GAANNNNTTC 2 cut(s) 723, 1196
AspLEI GCGC 4 cut(s) 65, 144, 999, 3144
AspS9I GGNCC 2 cut(s) 44, 151
AsuC2I CCSGG 1 cut(s) 1491
AsuHPI GGTGA 4 cut(s) 806, 971, 2579, 2664
AsuII TTCGAA 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 790
AvaII GGWCC 1 cut(s) 151
BalI TGGCCA 1 cut(s) 555
BamHI GGATCC 2 cut(s) 1624, 2413
BbsI GAAGAC 2 cut(s) 1533, 2981
BbvI GCAGC 6 cut(s) 45, 910, 2269, 2470, 2473, 2533
BceAI ACGGC 5 cut(s) 54, 885, 896, 2115, 3129
BciT130I CCWGG 6 cut(s) 23, 557, 1594, 2142, 2755, 2910
BciVI GTATCC 1 cut(s) 1137
BclI TGATCA 2 cut(s) 295, 1179
BcnI CCSGG 1 cut(s) 1491
BfaI CTAG 8 cut(s) 329, 839, 1061, 1163, 1523, 1757, 2492, 3201
BfmI CTRYAG 2 cut(s) 453, 462
BfoI RGCGCY 3 cut(s) 66, 145, 1000
BfrI CTTAAG 1 cut(s) 656
BfuI GTATCC 1 cut(s) 1137
BglI GCCNNNNNGGC 2 cut(s) 75, 132
BglII AGATCT 2 cut(s) 1405, 2719
Bme1390I CCNGG 7 cut(s) 23, 557, 1491, 1594, 2142, 2755, 2910
Bme18I GGWCC 1 cut(s) 151
BmeT110I CYCGRG 1 cut(s) 790
BmgBI CACGTC 1 cut(s) 857
BmgT120I GGNCC 2 cut(s) 44, 151
BmiI GGNNCC 7 cut(s) 152, 153, 384, 427, 615, 1626, 2415
BmrFI CCNGG 7 cut(s) 23, 557, 1491, 1594, 2142, 2755, 2910
BmsI GCATC 8 cut(s) 199, 1230, 2041, 2170, 2421, 2533, 2611, 3069
BpiI GAAGAC 2 cut(s) 1533, 2981
BpmI CTGGAG 3 cut(s) 1053, 2163, 3084
Bpu14I TTCGAA 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 1491
BsaBI GATNNNNATC 2 cut(s) 1713, 2904
BsaHI GRCGYC 2 cut(s) 542, 1399
BsaI GGTCTC 2 cut(s) 1377, 2496
BsaJI CCNNGG 9 cut(s) 22, 55, 70, 100, 127, 556, 1806, 1912, 2754
BsaWI WCCGGW 1 cut(s) 2214
BsaXI ACNNNNNCTCC 2 cut(s) 2786, 2816
Bsc4I CCNNNNNNNGG 7 cut(s) 942, 1324, 1599, 1949, 2423, 2791, 3190
Bse1I ACTGG 7 cut(s) 990, 1036, 1189, 1312, 1864, 2675, 3067
Bse3DI GCAATG 3 cut(s) 708, 1974, 2190
Bse8I GATNNNNATC 2 cut(s) 1713, 2904
BseAI TCCGGA 1 cut(s) 2214
BseBI CCWGG 6 cut(s) 23, 557, 1594, 2142, 2755, 2910
BseDI CCNNGG 9 cut(s) 22, 55, 70, 100, 127, 556, 1806, 1912, 2754
BseGI GGATG 5 cut(s) 237, 1873, 2618, 2905, 3199
BseJI GATNNNNATC 2 cut(s) 1713, 2904
BseLI CCNNNNNNNGG 7 cut(s) 942, 1324, 1599, 1949, 2423, 2791, 3190
BseMI GCAATG 3 cut(s) 708, 1974, 2190
BseMII CTCAG 8 cut(s) 167, 509, 738, 746, 842, 861, 905, 1914
BseNI ACTGG 7 cut(s) 990, 1036, 1189, 1312, 1864, 2675, 3067
BseRI GAGGAG 5 cut(s) 117, 120, 123, 1186, 2384
BseX3I CGGCCG 1 cut(s) 124
BseXI GCAGC 6 cut(s) 45, 910, 2269, 2470, 2473, 2533
BseYI CCCAGC 4 cut(s) 599, 1863, 2819, 3184
BsgI GTGCAG 2 cut(s) 2861, 3090
Bsh1236I CGCG 3 cut(s) 57, 87, 117
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 7 cut(s) 30, 45, 99, 126, 320, 555, 2130
BsiEI CGRYCG 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 790
BsiSI CCGG 2 cut(s) 1490, 2215
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 7 cut(s) 942, 1324, 1599, 1949, 2423, 2791, 3190
BsmBI CGTCTC 1 cut(s) 2107
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 3 cut(s) 731, 1148, 2870
BsnI GGCC 7 cut(s) 30, 45, 99, 126, 320, 555, 2130
Bso31I GGTCTC 2 cut(s) 1377, 2496
BsoBI CYCGRG 1 cut(s) 790
Bsp119I TTCGAA 1 cut(s) 445
Bsp13I TCCGGA 1 cut(s) 2214
Bsp1407I TGTACA 2 cut(s) 2399, 3256
BspANI GGCC 7 cut(s) 30, 45, 99, 126, 320, 555, 2130
BspCNI CTCAG 8 cut(s) 168, 508, 739, 747, 841, 862, 904, 1915
BspEI TCCGGA 1 cut(s) 2214
BspFNI CGCG 3 cut(s) 57, 87, 117
BspHI TCATGA 1 cut(s) 2176
BspLI GGNNCC 7 cut(s) 152, 153, 384, 427, 615, 1626, 2415
BspMAI CTGCAG 1 cut(s) 466
BspPI GGATC 9 cut(s) 1619, 1632, 1913, 2281, 2408, 2421, 2550, 2644, 2920
BspT104I TTCGAA 1 cut(s) 445
BspTI CTTAAG 1 cut(s) 656
BspTNI GGTCTC 2 cut(s) 1377, 2496
BsrBI CCGCTC 2 cut(s) 167, 254
BsrDI GCAATG 3 cut(s) 708, 1974, 2190
BsrGI TGTACA 2 cut(s) 2399, 3256
BsrI ACTGG 7 cut(s) 990, 1036, 1189, 1312, 1864, 2675, 3067
BssECI CCNNGG 9 cut(s) 22, 55, 70, 100, 127, 556, 1806, 1912, 2754
BssNAI GTATAC 1 cut(s) 2959
BssNI GRCGYC 2 cut(s) 542, 1399
BssT1I CCWWGG 1 cut(s) 1912
Bst1107I GTATAC 1 cut(s) 2959
Bst2UI CCWGG 6 cut(s) 23, 557, 1594, 2142, 2755, 2910
Bst4CI ACNGT 7 cut(s) 577, 625, 967, 1302, 1807, 2024, 2963
Bst6I CTCTTC 1 cut(s) 2848
BstACI GRCGYC 2 cut(s) 542, 1399
BstAFI CTTAAG 1 cut(s) 656
BstAUI TGTACA 2 cut(s) 2399, 3256
BstBI TTCGAA 1 cut(s) 445
BstC8I GCNNGC 8 cut(s) 67, 165, 516, 1965, 2124, 2128, 2926, 2983
BstDEI CTNAG 9 cut(s) 176, 495, 644, 747, 755, 828, 870, 891, 1923
BstDSI CCRYGG 3 cut(s) 55, 70, 1806
BstEII GGTNACC 1 cut(s) 3089
BstENI CCTNNNNNAGG 1 cut(s) 940
BstF5I GGATG 5 cut(s) 237, 1873, 2618, 2905, 3199
BstFNI CGCG 3 cut(s) 57, 87, 117
BstH2I RGCGCY 3 cut(s) 66, 145, 1000
BstHHI GCGC 4 cut(s) 65, 144, 999, 3144
BstMCI CGRYCG 1 cut(s) 127
BstNI CCWGG 6 cut(s) 23, 557, 1594, 2142, 2755, 2910
BstPI GGTNACC 1 cut(s) 3089
BstSCI CCNGG 7 cut(s) 21, 555, 1489, 1592, 2140, 2753, 2908
BstSFI CTRYAG 2 cut(s) 453, 462
BstUI CGCG 3 cut(s) 57, 87, 117
BstV1I GCAGC 6 cut(s) 45, 910, 2269, 2470, 2473, 2533
BstV2I GAAGAC 2 cut(s) 1533, 2981
BstX2I RGATCY 6 cut(s) 1405, 1624, 1918, 2413, 2555, 2719
BstYI RGATCY 6 cut(s) 1405, 1624, 1918, 2413, 2555, 2719
BstZ17I GTATAC 1 cut(s) 2959
BstZI CGGCCG 1 cut(s) 124
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 7 cut(s) 30, 45, 99, 126, 320, 555, 2130
BtgI CCRYGG 3 cut(s) 55, 70, 1806
BtrI CACGTC 1 cut(s) 857
BtsCI GGATG 5 cut(s) 237, 1873, 2618, 2905, 3199
BtsI GCAGTG 1 cut(s) 2849
BtsIMutI CAGTG 4 cut(s) 543, 1182, 2849, 2968
Cac8I GCNNGC 8 cut(s) 67, 165, 516, 1965, 2124, 2128, 2926, 2983
CaiI CAGNNNCTG 1 cut(s) 461
CciI TCATGA 1 cut(s) 2176
CfoI GCGC 4 cut(s) 65, 144, 999, 3144
Cfr13I GGNCC 2 cut(s) 44, 151
Cfr42I CCGCGG 1 cut(s) 58
CseI GACGC 2 cut(s) 123, 531
Csp6I GTAC 2 cut(s) 2400, 3257
CviQI GTAC 2 cut(s) 2400, 3257
DdeI CTNAG 9 cut(s) 176, 495, 644, 747, 755, 828, 870, 891, 1923
DraI TTTAAA 3 cut(s) 1242, 2058, 3165
EaeI YGGCCR 4 cut(s) 28, 124, 553, 2128
EagI CGGCCG 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 2848
EarI CTCTTC 1 cut(s) 2848
EclXI CGGCCG 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 1912
Eco31I GGTCTC 2 cut(s) 1377, 2496
Eco32I GATATC 1 cut(s) 2902
Eco47I GGWCC 1 cut(s) 151
Eco47III AGCGCT 2 cut(s) 143, 998
Eco52I CGGCCG 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 2085
Eco88I CYCGRG 1 cut(s) 790
Eco91I GGTNACC 1 cut(s) 3089
EcoNI CCTNNNNNAGG 1 cut(s) 940
EcoO109I RGGNCCY 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 3089
EcoRII CCWGG 6 cut(s) 21, 555, 1592, 2140, 2753, 2908
EcoRV GATATC 1 cut(s) 2902
EcoT14I CCWWGG 1 cut(s) 1912
EcoT22I ATGCAT 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 1912
Esp3I CGTCTC 1 cut(s) 2107
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 1613, 1645
FaqI GGGAC 1 cut(s) 164
FauI CCCGC 2 cut(s) 62, 614
FbaI TGATCA 2 cut(s) 295, 1179
FblI GTMKAC 3 cut(s) 452, 2745, 2958
FokI GGATG 5 cut(s) 244, 1880, 2625, 2892, 3206
FspBI CTAG 8 cut(s) 329, 839, 1061, 1163, 1523, 1757, 2492, 3201
GlaI GCGC 4 cut(s) 64, 143, 998, 3143
GsaI CCCAGC 4 cut(s) 603, 1867, 2823, 3188
GsuI CTGGAG 3 cut(s) 1053, 2163, 3084
HaeII RGCGCY 3 cut(s) 66, 145, 1000
HaeIII GGCC 7 cut(s) 30, 45, 99, 126, 320, 555, 2130
HapII CCGG 2 cut(s) 1490, 2215
HgaI GACGC 2 cut(s) 123, 531
HhaI GCGC 4 cut(s) 65, 144, 999, 3144
Hin1I GRCGYC 2 cut(s) 542, 1399
Hin6I GCGC 4 cut(s) 63, 142, 997, 3142
HinP1I GCGC 4 cut(s) 63, 142, 997, 3142
HincII GTYRAC 2 cut(s) 1305, 2746
HindII GTYRAC 2 cut(s) 1305, 2746
HindIII AAGCTT 5 cut(s) 516, 722, 1761, 2601, 3158
HpaI GTTAAC 1 cut(s) 1305
HpaII CCGG 2 cut(s) 1490, 2215
HphI GGTGA 4 cut(s) 806, 971, 2579, 2664
Hpy166II GTNNAC 8 cut(s) 453, 1305, 1775, 2402, 2746, 2959, 3149, 3259
Hpy8I GTNNAC 8 cut(s) 453, 1305, 1775, 2402, 2746, 2959, 3149, 3259
Hpy99I CGWCG 2 cut(s) 279, 1401
HpyAV CCTTC 9 cut(s) 355, 659, 673, 1131, 1397, 1593, 1615, 2603, 3104
HpyCH4III ACNGT 7 cut(s) 577, 625, 967, 1302, 1807, 2024, 2963
HpyCH4IV ACGT 5 cut(s) 277, 856, 1399, 1499, 1530
HpyF3I CTNAG 9 cut(s) 176, 495, 644, 747, 755, 828, 870, 891, 1923
HpySE526I ACGT 5 cut(s) 277, 856, 1399, 1499, 1530
Hsp92I GRCGYC 2 cut(s) 542, 1399
HspAI GCGC 4 cut(s) 63, 142, 997, 3142
KflI GGGWCCC 1 cut(s) 151
Kpn2I TCCGGA 1 cut(s) 2214
Ksp22I TGATCA 2 cut(s) 295, 1179
KspAI GTTAAC 1 cut(s) 1305
KspI CCGCGG 1 cut(s) 58
Lsp1109I GCAGC 6 cut(s) 45, 910, 2269, 2470, 2473, 2533
LweI GCATC 8 cut(s) 199, 1230, 2041, 2170, 2421, 2533, 2611, 3069
MaeI CTAG 8 cut(s) 329, 839, 1061, 1163, 1523, 1757, 2492, 3201
MaeII ACGT 5 cut(s) 277, 856, 1399, 1499, 1530
MaeIII GTNAC 6 cut(s) 1730, 1881, 2150, 2199, 2677, 3089
MbiI CCGCTC 2 cut(s) 167, 254
MboII GAAGA 9 cut(s) 854, 1222, 1382, 1538, 1709, 1931, 2865, 2986, 3184
MfeI CAATTG 1 cut(s) 2478
MflI RGATCY 6 cut(s) 1405, 1624, 1918, 2413, 2555, 2719
MlsI TGGCCA 1 cut(s) 555
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 6 cut(s) 480, 487, 671, 1574, 1607, 2993
MmeI TCCRAC 4 cut(s) 1593, 2161, 2251, 3077
Mox20I TGGCCA 1 cut(s) 555
Mph1103I ATGCAT 1 cut(s) 349
MroI TCCGGA 1 cut(s) 2214
MroXI GAANNNNTTC 2 cut(s) 723, 1196
MscI TGGCCA 1 cut(s) 555
MslI CAYNNNNRTG 2 cut(s) 342, 3077
Msp20I TGGCCA 1 cut(s) 555
MspA1I CMGCKG 1 cut(s) 57
MspCI CTTAAG 1 cut(s) 656
MspI CCGG 2 cut(s) 1490, 2215
MspR9I CCNGG 7 cut(s) 23, 557, 1491, 1594, 2142, 2755, 2910
MunI CAATTG 1 cut(s) 2478
Mva1269I GAATGC 3 cut(s) 731, 1148, 2870
MvaI CCWGG 6 cut(s) 23, 557, 1594, 2142, 2755, 2910
MvnI CGCG 3 cut(s) 57, 87, 117
NciI CCSGG 1 cut(s) 1491
NlaIV GGNNCC 7 cut(s) 152, 153, 384, 427, 615, 1626, 2415
NmeAIII GCCGAG 4 cut(s) 125, 152, 217, 1186
NsiI ATGCAT 1 cut(s) 349
NspV TTCGAA 1 cut(s) 445
PagI TCATGA 1 cut(s) 2176
PcsI WCGNNNNNNNCGW 1 cut(s) 1051
PctI GAATGC 3 cut(s) 731, 1148, 2870
PdmI GAANNNNTTC 2 cut(s) 723, 1196
PfeI GAWTC 9 cut(s) 442, 952, 979, 989, 1908, 2173, 2306, 2570, 3130
PflMI CCANNNNNTGG 3 cut(s) 1324, 2423, 2791
PfoI TCCNGGA 2 cut(s) 1592, 2908
PleI GAGTC 6 cut(s) 479, 486, 670, 1573, 1607, 2993
PpsI GAGTC 6 cut(s) 479, 486, 670, 1573, 1607, 2993
PpuMI RGGWCCY 1 cut(s) 151
Psp1406I AACGTT 1 cut(s) 1499
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 6 cut(s) 21, 555, 1592, 2140, 2753, 2908
PspEI GGTNACC 1 cut(s) 3089
PspFI CCCAGC 4 cut(s) 599, 1863, 2819, 3184
PspGI CCWGG 6 cut(s) 21, 555, 1592, 2140, 2753, 2908
PspN4I GGNNCC 7 cut(s) 152, 153, 384, 427, 615, 1626, 2415
PspPI GGNCC 2 cut(s) 44, 151
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 6 cut(s) 1405, 1624, 1918, 2413, 2555, 2719
RsaI GTAC 2 cut(s) 2401, 3258
RsaNI GTAC 2 cut(s) 2400, 3257
RseI CAYNNNNRTG 2 cut(s) 342, 3077
SacII CCGCGG 1 cut(s) 58
SalI GTCGAC 1 cut(s) 2744
Sau96I GGNCC 2 cut(s) 44, 151
SchI GAGTC 6 cut(s) 480, 487, 671, 1574, 1607, 2993
ScrFI CCNGG 7 cut(s) 23, 557, 1491, 1594, 2142, 2755, 2910
SfaNI GCATC 8 cut(s) 199, 1230, 2041, 2170, 2421, 2533, 2611, 3069
SfcI CTRYAG 2 cut(s) 453, 462
Sfr303I CCGCGG 1 cut(s) 58
SfuI TTCGAA 1 cut(s) 445
SgrBI CCGCGG 1 cut(s) 58
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 342, 3077
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
SspMI CTAG 8 cut(s) 329, 839, 1061, 1163, 1523, 1757, 2492, 3201
StyD4I CCNGG 7 cut(s) 21, 555, 1489, 1592, 2140, 2753, 2908
StyI CCWWGG 1 cut(s) 1912
TaaI ACNGT 7 cut(s) 577, 625, 967, 1302, 1807, 2024, 2963
TaiI ACGT 5 cut(s) 280, 859, 1402, 1502, 1533
TaqI TCGA 8 cut(s) 207, 445, 982, 1396, 2331, 2500, 2729, 2745
TatI WGTACW 2 cut(s) 2399, 3256
TfiI GAWTC 9 cut(s) 442, 952, 979, 989, 1908, 2173, 2306, 2570, 3130
TscAI CASTG 4 cut(s) 543, 1189, 2849, 2968
TseI GCWGC 6 cut(s) 33, 923, 2257, 2483, 2486, 2546
TspRI CASTG 4 cut(s) 543, 1189, 2849, 2968
Van91I CCANNNNNTGG 3 cut(s) 1324, 2423, 2791
Vha464I CTTAAG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 151
XagI CCTNNNNNAGG 1 cut(s) 940
XapI RAATTY 3 cut(s) 1588, 1673, 2208
XbaI TCTAGA 3 cut(s) 838, 1522, 1756
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmiI GTMKAC 3 cut(s) 452, 2745, 2958
XmnI GAANNNNTTC 2 cut(s) 723, 1196
XspI CTAG 8 cut(s) 329, 839, 1061, 1163, 1523, 1757, 2492, 3201
ZraI GACGTC 1 cut(s) 1400
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.