Rorug03G0153300

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007834
Physical Location & Seq
Forward (+)
12579415 .. 12581363
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0153300.1

Sequence Viewer

Length: 558 bp
ATGGGAACAGGGTGCTCACGATATGAGGCCTCACAGTCACAGCAGCTGAGACCTGGAGAACCAAGTAACAAGAAGTACTGGGTTGACAAGAAGTCCAACAACAGCTTCATGGTGTATCCAAGGGATCTCACAATCATTTGGGCTGAAGACAAACAGTACTGGCACTGGCCTTCCCTGGAAGAAACAAGTAATGTCTTTATCGATGTCGCTGAACTGTTAAAGGTCTGCTGGCTTCAAGTTCATGGAAAAATTGATTCCACAAAGCTGTCACCAGGAACCATATATGAAGTAGTATTTATAGTGATGTTGAAAGCTGCAGCTTATGGATGGAAAGTTCCTGTCAATGTCAGCCTCACTCTCCCAGATGGTAACAAGCAAGAACGTAAAGCTGATCTGAATAAAATCCCTAGAGAACAATGGACAGAGATACCAGTAGGCGAGTTTAGAGCATCAACAGGATTACTTGGGAACATTGAATTTTCGATGTATGAATATGAAGATGGGAAATGGAAGAGTGGACTTCTGATCAAGGGCGTTGCCGTTCGGCCTAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

185

Amino Acids

21.17

Weight (kDa)

8.41

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 34 - 183 1.2e-43 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 132
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 2 cut(s) 77, 158
AgsI TTSAA 3 cut(s) 236, 310, 476
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 52, 174, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
AluBI AGCT 6 cut(s) 46, 105, 265, 314, 320, 389
AluI AGCT 6 cut(s) 46, 105, 265, 314, 320, 389
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 1 cut(s) 132
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 3 cut(s) 27, 167, 545
ApeKI GCWGC 3 cut(s) 43, 314, 317
ApoI RAATTY 1 cut(s) 476
AsuHPI GGTGA 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 55, 301, 329
BccI CCATC 3 cut(s) 321, 359, 494
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 3 cut(s) 54, 176, 273
BciVI GTATCC 1 cut(s) 126
BclI TGATCA 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 315
BfuI GTATCC 1 cut(s) 126
BisI GCNGC 3 cut(s) 44, 315, 318
BlsI GCNGC 3 cut(s) 45, 316, 319
BmcAI AGTACT 2 cut(s) 77, 158
Bme1390I CCNGG 3 cut(s) 54, 176, 273
BmeRI GACNNNNNGTC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 3 cut(s) 54, 176, 273
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 458
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 75
Bsa29I ATCGAT 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 2 cut(s) 119, 174
Bse1I ACTGG 4 cut(s) 83, 164, 170, 431
BseBI CCWGG 3 cut(s) 54, 176, 273
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 119, 174
BseGI GGATG 1 cut(s) 332
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 4 cut(s) 83, 164, 170, 431
BseXI GCAGC 3 cut(s) 55, 301, 329
BshFI GGCC 3 cut(s) 29, 169, 547
BshVI ATCGAT 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 43
BsnI GGCC 3 cut(s) 29, 169, 547
Bso31I GGTCTC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 3 cut(s) 124, 391, 525
BspANI GGCC 3 cut(s) 29, 169, 547
BspCNI CTCAG 1 cut(s) 39
BspDI ATCGAT 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 1 cut(s) 132
BspTNI GGTCTC 1 cut(s) 43
BsrI ACTGG 4 cut(s) 83, 164, 170, 431
BssECI CCNNGG 2 cut(s) 119, 174
BssMI GATC 3 cut(s) 124, 391, 525
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 3 cut(s) 54, 176, 273
Bst4CI ACNGT 3 cut(s) 36, 156, 216
Bst6I CTCTTC 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 332
BstKTI GATC 3 cut(s) 127, 394, 528
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 3 cut(s) 124, 391, 525
BstNI CCWGG 3 cut(s) 54, 176, 273
BstSCI CCNGG 3 cut(s) 52, 174, 271
BstSFI CTRYAG 1 cut(s) 315
BstV1I GCAGC 3 cut(s) 55, 301, 329
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
Bsu15I ATCGAT 1 cut(s) 201
BsuI GTATCC 1 cut(s) 126
BsuRI GGCC 3 cut(s) 29, 169, 547
BsuTUI ATCGAT 1 cut(s) 201
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 230
CaiI CAGNNNCTG 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 201
Csp6I GTAC 2 cut(s) 76, 157
CviAII CATG 2 cut(s) 109, 242
CviQI GTAC 2 cut(s) 76, 157
DdeI CTNAG 1 cut(s) 47
DpnI GATC 3 cut(s) 126, 393, 527
DpnII GATC 3 cut(s) 124, 391, 525
DriI GACNNNNNGTC 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 506
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 119
Eco147I AGGCCT 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 165
EcoRII CCWGG 3 cut(s) 52, 174, 271
EcoT14I CCWWGG 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 2 cut(s) 112, 245
FatI CATG 2 cut(s) 108, 241
FbaI TGATCA 1 cut(s) 525
Fnu4HI GCNGC 3 cut(s) 44, 315, 318
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 44, 315, 318
FspBI CTAG 1 cut(s) 408
GluI GCNGC 3 cut(s) 44, 315, 318
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 3 cut(s) 29, 169, 547
Hin1II CATG 2 cut(s) 112, 245
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 1 cut(s) 254
HphI GGTGA 1 cut(s) 261
Hpy166II GTNNAC 2 cut(s) 85, 518
Hpy188I TCNGA 2 cut(s) 396, 525
Hpy188III TCNNGA 1 cut(s) 18
Hpy8I GTNNAC 2 cut(s) 85, 518
HpyAV CCTTC 1 cut(s) 180
HpyCH4III ACNGT 3 cut(s) 36, 156, 216
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 1 cut(s) 317
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 2 cut(s) 112, 245
Ksp22I TGATCA 1 cut(s) 525
Kzo9I GATC 3 cut(s) 124, 391, 525
Lsp1109I GCAGC 3 cut(s) 55, 301, 329
LweI GCATC 1 cut(s) 458
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 4 cut(s) 36, 65, 267, 368
MalI GATC 3 cut(s) 126, 393, 527
MboI GATC 3 cut(s) 124, 391, 525
MboII GAAGA 4 cut(s) 158, 191, 509, 523
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 2 cut(s) 249, 476
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 3 cut(s) 19, 40, 362
MseI TTAA 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 3 cut(s) 54, 176, 273
MvaI CCWGG 3 cut(s) 54, 176, 273
NdeII GATC 3 cut(s) 124, 391, 525
NlaIII CATG 2 cut(s) 112, 245
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 2 cut(s) 36, 267
PceI AGGCCT 1 cut(s) 29
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 3 cut(s) 45, 316, 319
Psp6I CCWGG 3 cut(s) 52, 174, 271
PspGI CCWGG 3 cut(s) 52, 174, 271
PspN4I GGNNCC 1 cut(s) 277
PstI CTGCAG 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 2 cut(s) 77, 158
RsaNI GTAC 2 cut(s) 76, 157
SaqAI TTAA 1 cut(s) 218
SatI GCNGC 3 cut(s) 44, 315, 318
Sau3AI GATC 3 cut(s) 124, 391, 525
ScaI AGTACT 2 cut(s) 77, 158
ScrFI CCNGG 3 cut(s) 54, 176, 273
SduI GDGCHC 1 cut(s) 17
SetI ASST 9 cut(s) 48, 55, 107, 225, 267, 316, 322, 385, 391
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 1 cut(s) 315
Sse9I AATT 2 cut(s) 249, 476
SseBI AGGCCT 1 cut(s) 29
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 52, 174, 271
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 3 cut(s) 36, 156, 216
TaiI ACGT 1 cut(s) 385
TaqI TCGA 2 cut(s) 201, 482
TasI AATT 2 cut(s) 249, 476
TatI WGTACW 2 cut(s) 75, 156
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 218
Tru9I TTAA 1 cut(s) 218
TscAI CASTG 1 cut(s) 170
TseFI GTSAC 2 cut(s) 36, 267
TseI GCWGC 3 cut(s) 43, 314, 317
Tsp45I GTSAC 2 cut(s) 36, 267
TspDTI ATGAA 5 cut(s) 97, 230, 300, 504, 510
TspRI CASTG 1 cut(s) 170
XapI RAATTY 1 cut(s) 476
XspI CTAG 1 cut(s) 408
ZrmI AGTACT 2 cut(s) 77, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.