RchiOBHm_Chr3g0476281

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007834
Physical Location & Seq
Reverse (-)
22228158 .. 22231808
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44171

Sequence Viewer

Length: 1614 bp
ATGTTGATATTGATCTTCACTACAAAAGTTATAGCACATCTACAAGAAAGTCTCTGGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGTGGAGGCAACTGTGGAACATATATGTCGCTATGAGAGAGATGGAGCAGTTCTTGTTTTCCTTACGGGCTGGGATGATATATCAAAGTTACTTGACAAAGTTAAGGGAAATAGATTCCTTGGAGATCCCGAGAAGTTCATGGTTCTTCCCCTGCATGGGTCAATGCCTTCTATCAATCAACGAGAAATATTTGATCGTCCTCCCACTAACAAAAGAAAAATTGTGCTAGCAACAAACATTGCAGAGAGCAGTATTACCATAGATGATGTCGTGTATGTCATAGACTGCGGAAAGGCAAAAGAAACGAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCATCATGGATTTCAAAGGCTTCAGCACATCAGAGACGAGGGCGTGCTGGCCGTGTCCAACCTGGAGTTTGTTACAGACTATATCCAAAAATGATTCACGATGCAATGCTCCAATATCAGTTACCTGAAATTCTCCGGACACCTTTGCAAGAGTTATGCCTCCATATAAAGAGTTTGCAGCTTGGAGCTGTTGGATCATTTTTGGCAAAGGCACTTCAACCACCAGATTCTCTTGCAGTTCAAAATGCCATCGAACTTCTCAAAACTATTGGAGCTTTAGATGATAGGGAGGAGCTTACTCCGCTTGGTCGCCATCTTTGTACACTACCATTGGATCCAAACATTGGAAAGATGCTTTTAATGGGTTCTATCTTTCAATGCCTCAATCCTGCCTTAACAATTGCTGCTGCCTTAGCTCATCGAGACCCATTTATCCTACCAATAGATAGGAAAGAGGAAGCTGATGCTGCAAAAAGATCCTTTGCTGGTGATTCTTTCAGTGATCACATAGCAGTTGTTAAAGCTTTTGAAGGATGGAAAGATGCAAAACATAATGGATCAGGAAAATCATTCTGTTGGGATAACTTTTTATCACCAGTAACCTTGCAGATGATGGAGGATATGAGGTTGCAGTTTGTAGATCTATTATCGAACATTGGCTTTGTCGACAAATCCAGGGGTGCTAACGCTTACAACCAATACAGCCATGACTTGGAGATGGTGTCTGCAATTCTTTGTGCTGGGCTTTACCCAAATGTTGTGCAGTGTAAAAGAAGAGGAAAGCGAACAACATTCTACACCAAAGAAGTTGGTAAAGTCGATATCCATCCTGGATCAGTCAATGCTGGGGTTCACTTATTCCCTCTGCCTTACATGGTATATAGTGAAAAAGTGAAGACAGCCAGCATTTACATCAGAGACTCTACAAATATATCAGATTATTCTTTGCTTTTGTTTGGTGGTAATCTCATTCCAACCAAAACTGGAGGGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTGGAGTTGATTCGGAAATTGCGCCGTGAACTCGACAAGCTTTTAAACAGGAAGATTGACAACCCAGCATTGGATGTCTCTAGTGAGGGAAAGGGAGTAGTATCTGCTGTGGTTGAGCTATTGCATAGCCCAAATGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

537

Amino Acids

59.73

Weight (kDa)

8.63

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 35 - 162 4.2e-16 Helicase conserved C-terminal domain
WHD_HA2 PF04408 226 - 254 4.8e-10 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 255 - 314 1e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 385 - 465 2.3e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 475 - 532 5.9e-22 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 773, 1141
AccI GTMKAC 1 cut(s) 1095
AccIII TCCGGA 1 cut(s) 564
AciI CCGC 2 cut(s) 375, 731
AclWI GGATC 7 cut(s) 206, 631, 758, 771, 900, 994, 1270
AcoI YGGCCR 1 cut(s) 478
AcsI RAATTY 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 2 cut(s) 751, 1608
AfiI CCNNNNNNNGG 5 cut(s) 242, 243, 773, 1141, 1540
AgsI TTSAA 5 cut(s) 444, 647, 671, 806, 959
AjnI CCWGG 3 cut(s) 490, 1103, 1258
Alw26I GTCTC 5 cut(s) 56, 457, 846, 1341, 1552
AlwI GGATC 7 cut(s) 206, 631, 758, 771, 900, 994, 1270
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 564
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 4 cut(s) 607, 833, 836, 896
ApoI RAATTY 1 cut(s) 558
AspLEI GCGC 1 cut(s) 1494
AsuHPI GGTGA 2 cut(s) 929, 1014
AsuNHI GCTAGC 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 215
BamHI GGATCC 1 cut(s) 763
BbsI GAAGAC 1 cut(s) 1331
BbvI GCAGC 4 cut(s) 619, 820, 823, 883
BccI CCATC 8 cut(s) 122, 439, 686, 750, 957, 1036, 1141, 1263
BceAI ACGGC 2 cut(s) 465, 1479
BciT130I CCWGG 3 cut(s) 492, 1105, 1260
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 5 cut(s) 56, 457, 846, 1341, 1552
BfaI CTAG 2 cut(s) 314, 1551
BglII AGATCT 1 cut(s) 1069
BisI GCNGC 4 cut(s) 608, 834, 837, 897
BlsI GCNGC 4 cut(s) 609, 835, 838, 898
Bme1390I CCNGG 3 cut(s) 492, 1105, 1260
BmeT110I CYCGRG 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 765
BmrFI CCNGG 3 cut(s) 492, 1105, 1260
BmsI GCATC 7 cut(s) 391, 520, 771, 883, 961, 1419, 1466
BmtI GCTAGC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 1331
BpmI CTGGAG 2 cut(s) 513, 1434
Bpu10I CCTNAGC 1 cut(s) 841
BsaBI GATNNNNATC 2 cut(s) 11, 1254
BsaI GGTCTC 1 cut(s) 846
BsaJI CCNNGG 2 cut(s) 205, 1104
BsaWI WCCGGW 1 cut(s) 564
BsaXI ACNNNNNCTCC 2 cut(s) 1136, 1166
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 243, 773, 1141, 1540
Bse1I ACTGG 2 cut(s) 1025, 1417
Bse3DI GCAATG 2 cut(s) 324, 540
Bse8I GATNNNNATC 2 cut(s) 11, 1254
BseAI TCCGGA 1 cut(s) 564
BseBI CCWGG 3 cut(s) 492, 1105, 1260
BseDI CCNNGG 2 cut(s) 205, 1104
BseGI GGATG 4 cut(s) 166, 968, 1255, 1549
BseJI GATNNNNATC 2 cut(s) 11, 1254
BseLI CCNNNNNNNGG 5 cut(s) 242, 243, 773, 1141, 1540
BseMI GCAATG 2 cut(s) 324, 540
BseNI ACTGG 2 cut(s) 1025, 1417
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 4 cut(s) 619, 820, 823, 883
BseYI CCCAGC 4 cut(s) 156, 1169, 1274, 1534
BsgI GTGCAG 1 cut(s) 1211
BshFI GGCC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 1 cut(s) 565
BslI CCNNNNNNNGG 5 cut(s) 242, 243, 773, 1141, 1540
BsmAI GTCTC 5 cut(s) 56, 457, 846, 1341, 1552
BsmBI CGTCTC 1 cut(s) 457
BsnI GGCC 1 cut(s) 480
Bso31I GGTCTC 1 cut(s) 846
BsoBI CYCGRG 1 cut(s) 215
Bsp13I TCCGGA 1 cut(s) 564
Bsp1407I TGTACA 2 cut(s) 749, 1606
BspACI CCGC 2 cut(s) 375, 731
BspANI GGCC 1 cut(s) 480
BspEI TCCGGA 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 765
BspOI GCTAGC 1 cut(s) 317
BspPI GGATC 7 cut(s) 206, 631, 758, 771, 900, 994, 1270
BspTNI GGTCTC 1 cut(s) 846
BsrDI GCAATG 2 cut(s) 324, 540
BsrGI TGTACA 2 cut(s) 749, 1606
BsrI ACTGG 2 cut(s) 1025, 1417
BssECI CCNNGG 2 cut(s) 205, 1104
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 3 cut(s) 492, 1105, 1260
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 1198
BstAUI TGTACA 2 cut(s) 749, 1606
BstC8I GCNNGC 4 cut(s) 315, 474, 478, 1333
BstDEI CTNAG 1 cut(s) 841
BstEII GGTNACC 1 cut(s) 1439
BstF5I GGATG 4 cut(s) 166, 968, 1255, 1549
BstHHI GCGC 1 cut(s) 1494
BstMAI GTCTC 5 cut(s) 56, 457, 846, 1341, 1552
BstMWI GCNNNNNNNGC 3 cut(s) 730, 842, 896
BstNI CCWGG 3 cut(s) 492, 1105, 1260
BstPI GGTNACC 1 cut(s) 1439
BstSCI CCNGG 3 cut(s) 490, 1103, 1258
BstV1I GCAGC 4 cut(s) 619, 820, 823, 883
BstV2I GAAGAC 1 cut(s) 1331
BstX2I RGATCY 4 cut(s) 211, 763, 905, 1069
BstYI RGATCY 4 cut(s) 211, 763, 905, 1069
BsuRI GGCC 1 cut(s) 480
BtsCI GGATG 4 cut(s) 166, 968, 1255, 1549
BtsI GCAGTG 1 cut(s) 1199
BtsIMutI CAGTG 2 cut(s) 934, 1199
Cac8I GCNNGC 4 cut(s) 315, 474, 478, 1333
CfoI GCGC 1 cut(s) 1494
Csp6I GTAC 2 cut(s) 750, 1607
CviAII CATG 5 cut(s) 226, 242, 435, 1136, 1303
CviQI GTAC 2 cut(s) 750, 1607
DdeI CTNAG 1 cut(s) 841
DraI TTTAAA 2 cut(s) 408, 1515
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 1198
EarI CTCTTC 1 cut(s) 1198
Eco130I CCWWGG 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 846
Eco32I GATATC 1 cut(s) 1252
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 215
Eco91I GGTNACC 1 cut(s) 1439
EcoO65I GGTNACC 1 cut(s) 1439
EcoRII CCWGG 3 cut(s) 490, 1103, 1258
EcoRV GATATC 1 cut(s) 1252
EcoT14I CCWWGG 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 5 cut(s) 229, 245, 438, 1139, 1306
FatI CATG 5 cut(s) 225, 241, 434, 1135, 1302
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 4 cut(s) 608, 834, 837, 897
FokI GGATG 4 cut(s) 173, 975, 1242, 1556
Fsp4HI GCNGC 4 cut(s) 608, 834, 837, 897
FspBI CTAG 2 cut(s) 314, 1551
GlaI GCGC 1 cut(s) 1493
GluI GCNGC 4 cut(s) 608, 834, 837, 897
GsaI CCCAGC 4 cut(s) 160, 1173, 1278, 1538
GsuI CTGGAG 2 cut(s) 513, 1434
HaeIII GGCC 1 cut(s) 480
HapII CCGG 1 cut(s) 565
HhaI GCGC 1 cut(s) 1494
Hin1II CATG 5 cut(s) 229, 245, 438, 1139, 1306
Hin6I GCGC 1 cut(s) 1492
HinP1I GCGC 1 cut(s) 1492
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 3 cut(s) 59, 951, 1508
HinfI GANTC 6 cut(s) 201, 523, 656, 920, 1349, 1480
HpaII CCGG 1 cut(s) 565
HphI GGTGA 2 cut(s) 929, 1014
Hpy166II GTNNAC 6 cut(s) 73, 752, 1096, 1282, 1499, 1609
Hpy188I TCNGA 4 cut(s) 462, 1346, 1366, 1485
Hpy188III TCNNGA 6 cut(s) 55, 215, 527, 565, 851, 990
Hpy8I GTNNAC 6 cut(s) 73, 752, 1096, 1282, 1499, 1609
HpyAV CCTTC 3 cut(s) 264, 953, 1454
HpyCH4III ACNGT 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 3 cut(s) 730, 842, 896
HpyF3I CTNAG 1 cut(s) 841
Hsp92II CATG 5 cut(s) 229, 245, 438, 1139, 1306
HspAI GCGC 1 cut(s) 1492
Kpn2I TCCGGA 1 cut(s) 564
Ksp22I TGATCA 1 cut(s) 931
LmnI GCTCC 5 cut(s) 131, 543, 614, 701, 721
Lsp1109I GCAGC 4 cut(s) 619, 820, 823, 883
LweI GCATC 7 cut(s) 391, 520, 771, 883, 961, 1419, 1466
MaeI CTAG 2 cut(s) 314, 1551
MaeIII GTNAC 5 cut(s) 174, 500, 549, 1027, 1439
MboII GAAGA 5 cut(s) 7, 224, 1215, 1336, 1534
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 4 cut(s) 211, 763, 905, 1069
MluCI AATT 5 cut(s) 306, 558, 828, 1158, 1487
MlyI GAGTC 1 cut(s) 1343
MmeI TCCRAC 3 cut(s) 511, 601, 1427
MroI TCCGGA 1 cut(s) 564
MseI TTAA 6 cut(s) 189, 407, 788, 824, 948, 1514
MslI CAYNNNNRTG 1 cut(s) 1427
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 3 cut(s) 492, 1105, 1260
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 3 cut(s) 492, 1105, 1260
MwoI GCNNNNNNNGC 3 cut(s) 730, 842, 896
NheI GCTAGC 1 cut(s) 313
NlaIII CATG 5 cut(s) 229, 245, 438, 1139, 1306
NlaIV GGNNCC 1 cut(s) 765
PfeI GAWTC 5 cut(s) 201, 523, 656, 920, 1480
PflMI CCANNNNNTGG 2 cut(s) 773, 1141
PfoI TCCNGGA 1 cut(s) 1258
PkrI GCNGC 4 cut(s) 609, 835, 838, 898
PleI GAGTC 1 cut(s) 1343
PpsI GAGTC 1 cut(s) 1343
Psp6I CCWGG 3 cut(s) 490, 1103, 1258
PspEI GGTNACC 1 cut(s) 1439
PspFI CCCAGC 4 cut(s) 156, 1169, 1274, 1534
PspGI CCWGG 3 cut(s) 490, 1103, 1258
PspN4I GGNNCC 1 cut(s) 765
PsuI RGATCY 4 cut(s) 211, 763, 905, 1069
RsaI GTAC 2 cut(s) 751, 1608
RsaNI GTAC 2 cut(s) 750, 1607
RseI CAYNNNNRTG 1 cut(s) 1427
SalI GTCGAC 1 cut(s) 1094
SaqAI TTAA 6 cut(s) 189, 407, 788, 824, 948, 1514
SatI GCNGC 4 cut(s) 608, 834, 837, 897
SchI GAGTC 1 cut(s) 1343
ScrFI CCNGG 3 cut(s) 492, 1105, 1260
SfaNI GCATC 7 cut(s) 391, 520, 771, 883, 961, 1419, 1466
SmiMI CAYNNNNRTG 1 cut(s) 1427
Sse9I AATT 5 cut(s) 306, 558, 828, 1158, 1487
SsiI CCGC 2 cut(s) 375, 731
SspI AATATT 1 cut(s) 276
SspMI CTAG 2 cut(s) 314, 1551
StyD4I CCNGG 3 cut(s) 490, 1103, 1258
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 6 cut(s) 681, 850, 1079, 1095, 1248, 1503
TasI AATT 5 cut(s) 306, 558, 828, 1158, 1487
TatI WGTACW 2 cut(s) 749, 1606
TfiI GAWTC 5 cut(s) 201, 523, 656, 920, 1480
Tru1I TTAA 6 cut(s) 189, 407, 788, 824, 948, 1514
Tru9I TTAA 6 cut(s) 189, 407, 788, 824, 948, 1514
TscAI CASTG 2 cut(s) 934, 1199
TseI GCWGC 4 cut(s) 607, 833, 836, 896
TspDTI ATGAA 2 cut(s) 214, 1436
TspRI CASTG 2 cut(s) 934, 1199
Van91I CCANNNNNTGG 2 cut(s) 773, 1141
XapI RAATTY 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 1095
XspI CTAG 2 cut(s) 314, 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.