MD12G1041500.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007834
Physical Location & Seq
Reverse (-)
4602120 .. 4608928
6809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041500.v1.1.491

Sequence Viewer

Length: 3114 bp
ATGTCAGTGCGCTTACTCCTTGGCGCAACAACAAGCTGCCCGCTTCCCCGACACCTCCTCGCCGCGCCCAAAATCTTCGCGTTTCGCCCTGGGATCTCGACACTCGCCATGTCCGGACGCACCAACTTCCAAGGCGGTCGCCGAGGCGGCCCTAACTCTGGCGGAGGCCGCCGCGGTGGCGGTCGCGGTGGCGGCGGCAGAGGTGGAGGCGGCCGCGGCGGAGAACAGCGCTGGTGGGACCCGGTATGGCGCGCAGAGCGACTGAGGCAGCAGGCTGTCCAGATGGAAGTGCTTGATGAGACCGAATGGTGGGGAAAGATGGAGCAGATGAAGAACGGAGCGGAGCAGGAGATGGTAATTAAGCGTAATTTTAGCCGGAATGATCAGCAAATTCTATATGACATGGCTTATCAACTGGGGCTTTACTTCCATGCATACAATAAAGGGAAGGCTTTGGTGGTCAGCAAAGTTCCATTGCCGGACTACCGGGCAGATCTTGATGAGCGCCATGGTTCCACACAGAAAGAGATTAAAATGTCTACAGAGACTGCCAATAGAGTTGGGAGTCTTTTGCACAGCTCGCCGTCACAGGGGGAAATATCTGTCAGTGGGCCTTCTGGGTCAGGGCAGGGAAACAGACAAACTTCAGCTAGCGTAATTACGAGTAAACCTGTAGCCCAGTTAGAGCCTGATAATGTTAATGAGAAAGAGAAACTCAGCCTTCAACTTAAGGAGAAACAGGAAAAAATGAAGGTGAGCAATAGTTTAAAAGCAATGCAATCGTTTCGAGAAAAGCTTCCTGCTTTCAAAATGAAGTCCGAGTTTCTGAAAGCTGTTTCAGAAAATCAGGTCTTGGTAGTCTCTGGGGAGACAGGTTGCGGAAAAACAACACAGCTTCCGCAATTTATTCTAGAAGATGAGATCTCACGTCTGCATGGTGCTGATTGCAACATAATATGCACACAACCTCGTCGTATTTCTGCTGTATCTGTTGCAGCTCGTATATCCTCTGAAAGGGGAGAGAATCTTGGTGAAACTGTTGGTTATCAGATCCGTCTTGAATCAAAGCGTACTGCTCAAACACGACTGTTATTTTGCACCACTGGAGTGTTACTTCGACAGTTGGTTCAAGACCCAGAATTAACGGGCGTGAGCCACTTGCTAGTTGATGAAATTCATGAAAGAGGCATGAATGAGGACTTTTTACTCATAATTTTGCGTGACCTTCTTCCTCGGCGTCCAGATCTACGATTAATTCTAATGAGTGCTACCATCAATGCCGACTTGTTCTCCAAATACTTTGGAAATTCCCCAACAATACATATACCAGGATTAACTTTTCCTGTGGCAGAGTTATTTCTTGAAGATATTCTGGAGAAAACTCGTTACGCTGTCAAGTCAGAGTATGACAACTTTGAGGGGGGAAATTCCAGGAGAAGGAGACAGCAAGATTCCAAGAAAGATCCTTTAACTGAATTATTTGAGGATGCCGATATTGATGTTCAGTTCAAAAATTACAGCACAGCTACAAGAAAGTCTCTTGAAGCTTGGTCTGGTTCACAGCTTGATTTGGGGCTGGTGGAGGCGACAATTGAACATATATGTCGCAATGAGAGAGATGGAGCAATTCTTGTATTCCTTACAGGCTGGGATGACATATCAAAGTTACTTGACAAAATTAAGGGAAATAGATTCCTTGGAGATCCCACAAAATATATGGTTCTTCCCCTGCATGGGTCAATGCCTACCGTCAATCAACGAGAGATATTTGATCGTCCTCCCCCTAATAAAAGAAAAATTGTGCTAGCAACAAACATAGCCGAGAGTAGTATCACCATAGATGATGTTGTGTATGTCATAGACTGCGGAAAGGCAAAGGAAACCAGTTATGATGCTTTAAACAAGTTGGCTTGTCTATTACCTTCATGGATTTCAAAGGCTTCAGCACATCAGAGGCGAGGGCGTGCTGGCCGTGTGCAACCTGGAGTTTGTTACAGACTATATCCGAAAATGATCCATGATGCAATGCTCCAATATCAATTACCTGAAATCCTCCGGACGCCTTTGCAAGAGCTATGCCTCCACATCAAAAGTTTGCAACTTGGAGCTGTTGGGTCATTTTTGGCAAAGGCACTTCAGCCACCAGATTCTCTTGCAGTTCAAAATGCAATTGAACTTCTCAAAACCATTGGAGCTTTAGATGACACAGAGGAGCTTACCCCGCTTGGTCGCCATCTTTGCACTCTACCATTGGATCCAAACATTGGGAAGATGCTTTTAATGGGTTCTATATTTCAATGCCTCAATCCTGCTTTAACAATTGCAGCTGCTCTCGCTCATCGTGACCCATTTGTACTACCAATAAATAGGAAAGAGGATGCTGATGCTGCAAAGAGGTCCTTTGCGGGTGATTCTTTCAGTGATCACATAGCTCTTGTGAAAGCTTTTGAAGGATGGAAAGATGCAAAACAAAATGGAGCGGGAGAAGGAAAGTCGTTCTGCTGGGAGAACTTTTTATCCCCTGTAACATTGCAGATGATGGAAGATATGAGGATCCAGTTTCTTGATCTATTATCAAATATTGGCTTTCTTGACAAATCCAGGGGTGCTAATGCTTACAATCAATACAGCCATGACTTGGAGATGGTGTGTGCGGTCCTGTGTGCTGGGCTCTATCCGAATGTCGTTCAGTGTAAAAGAAGAGGAAAGCGGACAGCATTCTATACTAAGGAAGTCGGTAAAGTTGATATCCATCCTGCATCAGTCAATGCTGGGGTTCATTTATTCCCTCTCCCTTACATGGTTTATAGTGAAAAGGTGAAGACAACCAGCATTTACATCCGAGACTCTACGAATTTATCAGATTATGCTCTACTTCTATTTGGTGGTAGTCTTATCCCTAGCAAGACCGGGGAGGGCATTGAGATGCTTGGAGGTTACCTTCATTTCTCTGCATCAAAGAGTGTTTTAGAATTGATTCGGAAACTGCGTGGTGAACTTGACAAGCTTTTGAACAGGAAGATTGACAACCCAGGATTGGACATCTCCAGCGAGGGAAAGGCAGTTGTGTCTGCTGTGGTAGAGTTGCTGCATAGTCAGAATGTACAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042623 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1038

Amino Acids

115.14

Weight (kDa)

8.77

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 273 - 427 1.3e-09 DEAD/DEAH box helicase
Helicase_C PF00271 529 - 659 1.7e-15 Helicase conserved C-terminal domain
WHD_HA2 PF04408 723 - 751 1.3e-09 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 752 - 812 1.2e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 883 - 964 2.5e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 974 - 1031 2.9e-22 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 2264, 2638
AccBSI CCGCTC 2 cut(s) 341, 2480
AccI GTMKAC 1 cut(s) 539
AccII CGCG 6 cut(s) 65, 80, 174, 186, 216, 252
AccIII TCCGGA 2 cut(s) 113, 2055
AclWI GGATC 9 cut(s) 101, 1045, 1457, 1697, 2008, 2249, 2262, 2548, 2561
AcoI YGGCCR 2 cut(s) 211, 1969
AcsI RAATTY 5 cut(s) 390, 1173, 1306, 1426, 2854
AcuI CTGAAG 3 cut(s) 630, 1926, 2120
AcyI GRCGYC 2 cut(s) 1237, 2060
AfaI GTAC 3 cut(s) 1072, 2355, 3105
AfeI AGCGCT 1 cut(s) 230
AflII CTTAAG 1 cut(s) 728
AjiI CACGTC 1 cut(s) 929
AjnI CCWGG 6 cut(s) 88, 1327, 1430, 1981, 2600, 3031
AleI CACNNNNGTG 1 cut(s) 1106
AloI GAACNNNNNNTCC 2 cut(s) 2501, 2533
Alw26I GTCTC 7 cut(s) 293, 539, 863, 865, 1435, 1542, 2838
AlwI GGATC 9 cut(s) 101, 1045, 1457, 1697, 2008, 2249, 2262, 2548, 2561
AlwNI CAGNNNCTG 1 cut(s) 548
Aor13HI TCCGGA 2 cut(s) 113, 2055
Aor51HI AGCGCT 1 cut(s) 230
AoxI GGCC 5 cut(s) 148, 166, 211, 611, 1969
ApeKI GCWGC 7 cut(s) 36, 268, 995, 2324, 2327, 2387, 3088
ApoI RAATTY 5 cut(s) 390, 1173, 1306, 1426, 2854
ArsI GACNNNNNNTTYG 2 cut(s) 1077, 1109
AseI ATTAAT 1 cut(s) 1253
Asp700I GAANNNNTTC 3 cut(s) 795, 1368, 2976
AspLEI GCGC 7 cut(s) 12, 26, 67, 231, 252, 254, 507
AspS9I GGNCC 5 cut(s) 149, 238, 611, 2397, 2656
AsuC2I CCSGG 3 cut(s) 242, 488, 2911
AsuHPI GGTGA 6 cut(s) 766, 1043, 1825, 2420, 2830, 3005
AsuNHI GCTAGC 2 cut(s) 650, 1804
AvaII GGWCC 3 cut(s) 238, 2397, 2656
BamHI GGATCC 2 cut(s) 2254, 2553
BanII GRGCYC 1 cut(s) 2673
BbsI GAAGAC 1 cut(s) 2828
BbvI GCAGC 7 cut(s) 23, 280, 1007, 2314, 2336, 2374, 3075
BceAI ACGGC 2 cut(s) 568, 1956
BcgI CGANNNNNNTGC 4 cut(s) 762, 767, 796, 801
BciT130I CCWGG 6 cut(s) 90, 1329, 1432, 1983, 2602, 3033
BclI TGATCA 2 cut(s) 382, 2422
BcnI CCSGG 3 cut(s) 242, 488, 2911
BcoDI GTCTC 7 cut(s) 293, 539, 863, 865, 1435, 1542, 2838
BfaI CTAG 5 cut(s) 651, 911, 1163, 1805, 2901
BfmI CTRYAG 2 cut(s) 540, 672
BfoI RGCGCY 2 cut(s) 232, 508
BfrI CTTAAG 1 cut(s) 728
BglI GCCNNNNNGGC 2 cut(s) 147, 177
BglII AGATCT 3 cut(s) 493, 921, 1243
Bme1390I CCNGG 9 cut(s) 90, 242, 488, 1329, 1432, 1983, 2602, 2911, 3033
Bme18I GGWCC 3 cut(s) 238, 2397, 2656
BmgBI CACGTC 1 cut(s) 929
BmgT120I GGNCC 5 cut(s) 149, 238, 611, 2397, 2656
BmiI GGNNCC 5 cut(s) 239, 240, 514, 2256, 2555
BmrFI CCNGG 9 cut(s) 90, 242, 488, 1329, 1432, 1983, 2602, 2911, 3033
BmrI ACTGGG 2 cut(s) 425, 673
BmtI GCTAGC 2 cut(s) 654, 1808
BmuI ACTGGG 2 cut(s) 425, 673
BpiI GAAGAC 1 cut(s) 2828
BpmI CTGGAG 4 cut(s) 1125, 1394, 2004, 3031
BpuMI CCSGG 3 cut(s) 242, 488, 2911
BsaBI GATNNNNATC 1 cut(s) 2751
BsaHI GRCGYC 2 cut(s) 1237, 2060
BsaI GGTCTC 1 cut(s) 293
BsaWI WCCGGW 2 cut(s) 113, 2055
BsaXI ACNNNNNCTCC 4 cut(s) 1274, 1304, 2633, 2663
Bse1I ACTGG 5 cut(s) 420, 679, 1108, 1884, 2557
Bse3DI GCAATG 5 cut(s) 473, 780, 1615, 2031, 2528
Bse8I GATNNNNATC 1 cut(s) 2751
BseAI TCCGGA 2 cut(s) 113, 2055
BseBI CCWGG 6 cut(s) 90, 1329, 1432, 1983, 2602, 3033
BseGI GGATG 6 cut(s) 1492, 1657, 2383, 2459, 2752, 2838
BseJI GATNNNNATC 1 cut(s) 2751
BseMI GCAATG 5 cut(s) 473, 780, 1615, 2031, 2528
BseMII CTCAG 2 cut(s) 254, 730
BseNI ACTGG 5 cut(s) 420, 679, 1108, 1884, 2557
BsePI GCGCGC 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 47, 2225
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 7 cut(s) 23, 280, 1007, 2314, 2336, 2374, 3075
BseYI CCCAGC 4 cut(s) 1647, 2502, 2666, 2771
Bsh1236I CGCG 6 cut(s) 65, 80, 174, 186, 216, 252
Bsh1285I CGRYCG 3 cut(s) 139, 184, 214
BshFI GGCC 5 cut(s) 150, 168, 213, 613, 1971
BsiEI CGRYCG 3 cut(s) 139, 184, 214
BsiSI CCGG 7 cut(s) 114, 242, 376, 479, 487, 2056, 2910
BslFI GGGAC 1 cut(s) 251
BsmAI GTCTC 7 cut(s) 293, 539, 863, 865, 1435, 1542, 2838
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 2717
BsnI GGCC 5 cut(s) 150, 168, 213, 613, 1971
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 2673
Bsp13I TCCGGA 2 cut(s) 113, 2055
Bsp1407I TGTACA 1 cut(s) 3103
Bsp19I CCATGG 1 cut(s) 508
BspANI GGCC 5 cut(s) 150, 168, 213, 613, 1971
BspCNI CTCAG 2 cut(s) 255, 729
BspEI TCCGGA 2 cut(s) 113, 2055
BspFNI CGCG 6 cut(s) 65, 80, 174, 186, 216, 252
BspHI TCATGA 1 cut(s) 1177
BspLI GGNNCC 5 cut(s) 239, 240, 514, 2256, 2555
BspOI GCTAGC 2 cut(s) 654, 1808
BspPI GGATC 9 cut(s) 101, 1045, 1457, 1697, 2008, 2249, 2262, 2548, 2561
BspTI CTTAAG 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 293
BsrBI CCGCTC 2 cut(s) 341, 2480
BsrDI GCAATG 5 cut(s) 473, 780, 1615, 2031, 2528
BsrGI TGTACA 1 cut(s) 3103
BsrI ACTGG 5 cut(s) 420, 679, 1108, 1884, 2557
BssHII GCGCGC 1 cut(s) 250
BssNI GRCGYC 2 cut(s) 1237, 2060
BssT1I CCWWGG 4 cut(s) 19, 130, 508, 1696
Bst2UI CCWGG 6 cut(s) 90, 1329, 1432, 1983, 2602, 3033
Bst4CI ACNGT 4 cut(s) 1039, 1089, 1122, 1750
Bst6I CTCTTC 1 cut(s) 2695
BstACI GRCGYC 2 cut(s) 1237, 2060
BstAFI CTTAAG 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 3103
BstC8I GCNNGC 8 cut(s) 41, 252, 273, 581, 652, 1806, 1965, 1969
BstDEI CTNAG 3 cut(s) 263, 716, 2727
BstDSI CCRYGG 3 cut(s) 172, 214, 508
BstEII GGTNACC 1 cut(s) 2936
BstENI CCTNNNNNAGG 1 cut(s) 1012
BstF5I GGATG 6 cut(s) 1492, 1657, 2383, 2459, 2752, 2838
BstFNI CGCG 6 cut(s) 65, 80, 174, 186, 216, 252
BstH2I RGCGCY 2 cut(s) 232, 508
BstHHI GCGC 7 cut(s) 12, 26, 67, 231, 252, 254, 507
BstMAI GTCTC 7 cut(s) 293, 539, 863, 865, 1435, 1542, 2838
BstMCI CGRYCG 3 cut(s) 139, 184, 214
BstNI CCWGG 6 cut(s) 90, 1329, 1432, 1983, 2602, 3033
BstPI GGTNACC 1 cut(s) 2936
BstSCI CCNGG 9 cut(s) 88, 240, 486, 1327, 1430, 1981, 2600, 2909, 3031
BstSFI CTRYAG 2 cut(s) 540, 672
BstUI CGCG 6 cut(s) 65, 80, 174, 186, 216, 252
BstV1I GCAGC 7 cut(s) 23, 280, 1007, 2314, 2336, 2374, 3075
BstV2I GAAGAC 1 cut(s) 2828
BstX2I RGATCY 9 cut(s) 93, 493, 921, 1050, 1243, 1462, 1702, 2254, 2553
BstYI RGATCY 9 cut(s) 93, 493, 921, 1050, 1243, 1462, 1702, 2254, 2553
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 5 cut(s) 150, 168, 213, 613, 1971
BtgI CCRYGG 3 cut(s) 172, 214, 508
BtrI CACGTC 1 cut(s) 929
BtsCI GGATG 6 cut(s) 1492, 1657, 2383, 2459, 2752, 2838
BtsIMutI CAGTG 5 cut(s) 12, 613, 1101, 2425, 2696
Cac8I GCNNGC 8 cut(s) 41, 252, 273, 581, 652, 1806, 1965, 1969
CaiI CAGNNNCTG 1 cut(s) 548
CciI TCATGA 1 cut(s) 1177
CciNI GCGGCCGC 1 cut(s) 211
CfoI GCGC 7 cut(s) 12, 26, 67, 231, 252, 254, 507
Cfr13I GGNCC 5 cut(s) 149, 238, 611, 2397, 2656
Cfr42I CCGCGG 2 cut(s) 175, 217
CseI GACGC 3 cut(s) 126, 1226, 2068
Csp6I GTAC 3 cut(s) 1071, 2354, 3104
CviQI GTAC 3 cut(s) 1071, 2354, 3104
DdeI CTNAG 3 cut(s) 263, 716, 2727
DraI TTTAAA 2 cut(s) 768, 1899
EaeI YGGCCR 2 cut(s) 211, 1969
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 2695
EarI CTCTTC 1 cut(s) 2695
EciI GGCGGA 2 cut(s) 177, 234
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 4 cut(s) 19, 130, 508, 1696
Eco24I GRGCYC 1 cut(s) 2673
Eco31I GGTCTC 1 cut(s) 293
Eco32I GATATC 1 cut(s) 2749
Eco47I GGWCC 3 cut(s) 238, 2397, 2656
Eco47III AGCGCT 1 cut(s) 230
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 3 cut(s) 630, 1926, 2120
Eco91I GGTNACC 1 cut(s) 2936
EcoNI CCTNNNNNAGG 1 cut(s) 1012
EcoO109I RGGNCCY 2 cut(s) 238, 2397
EcoO65I GGTNACC 1 cut(s) 2936
EcoRII CCWGG 6 cut(s) 88, 1327, 1430, 1981, 2600, 3031
EcoRV GATATC 1 cut(s) 2749
EcoT14I CCWWGG 4 cut(s) 19, 130, 508, 1696
EcoT22I ATGCAT 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 2673
ErhI CCWWGG 4 cut(s) 19, 130, 508, 1696
FalI AAGNNNNNCTT 2 cut(s) 2384, 2416
FaqI GGGAC 1 cut(s) 251
FauI CCCGC 4 cut(s) 48, 2229, 2398, 2473
FbaI TGATCA 2 cut(s) 382, 2422
FblI GTMKAC 1 cut(s) 539
FokI GGATG 6 cut(s) 1499, 1664, 2390, 2466, 2739, 2825
FriOI GRGCYC 1 cut(s) 2673
FspBI CTAG 5 cut(s) 651, 911, 1163, 1805, 2901
GlaI GCGC 7 cut(s) 11, 25, 66, 230, 251, 253, 506
GsaI CCCAGC 4 cut(s) 1651, 2506, 2670, 2775
GsuI CTGGAG 4 cut(s) 1125, 1394, 2004, 3031
HaeII RGCGCY 2 cut(s) 232, 508
HaeIII GGCC 5 cut(s) 150, 168, 213, 613, 1971
HapII CCGG 7 cut(s) 114, 242, 376, 479, 487, 2056, 2910
HgaI GACGC 3 cut(s) 126, 1226, 2068
HhaI GCGC 7 cut(s) 12, 26, 67, 231, 252, 254, 507
Hin1I GRCGYC 2 cut(s) 1237, 2060
Hin6I GCGC 7 cut(s) 10, 24, 65, 229, 250, 252, 505
HinP1I GCGC 7 cut(s) 10, 24, 65, 229, 250, 252, 505
HindIII AAGCTT 4 cut(s) 794, 1545, 2442, 3005
HinfI GANTC 9 cut(s) 565, 1024, 1061, 1451, 1692, 2147, 2411, 2846, 2977
HpaII CCGG 7 cut(s) 114, 242, 376, 479, 487, 2056, 2910
HphI GGTGA 6 cut(s) 766, 1043, 1825, 2420, 2830, 3005
Hpy166II GTNNAC 4 cut(s) 540, 668, 1559, 2996
Hpy8I GTNNAC 4 cut(s) 540, 668, 1559, 2996
Hpy99I CGWCG 1 cut(s) 975
HpyCH4III ACNGT 4 cut(s) 1039, 1089, 1122, 1750
HpyCH4IV ACGT 1 cut(s) 928
HpyF3I CTNAG 3 cut(s) 263, 716, 2727
HpySE526I ACGT 1 cut(s) 928
Hsp92I GRCGYC 2 cut(s) 1237, 2060
HspAI GCGC 7 cut(s) 10, 24, 65, 229, 250, 252, 505
KflI GGGWCCC 1 cut(s) 238
Kpn2I TCCGGA 2 cut(s) 113, 2055
Ksp22I TGATCA 2 cut(s) 382, 2422
KspI CCGCGG 2 cut(s) 175, 217
LmnI GCTCC 9 cut(s) 322, 338, 343, 1622, 2034, 2105, 2192, 2212, 2477
Lsp1109I GCAGC 7 cut(s) 23, 280, 1007, 2314, 2336, 2374, 3075
MaeI CTAG 5 cut(s) 651, 911, 1163, 1805, 2901
MaeII ACGT 1 cut(s) 928
MaeIII GTNAC 9 cut(s) 585, 1110, 1220, 1385, 1665, 1991, 2342, 2524, 2936
MbiI CCGCTC 2 cut(s) 341, 2480
MfeI CAATTG 3 cut(s) 1590, 2169, 2319
MflI RGATCY 9 cut(s) 93, 493, 921, 1050, 1243, 1462, 1702, 2254, 2553
MhlI GDGCHC 1 cut(s) 2673
MlyI GAGTC 2 cut(s) 574, 2840
Mph1103I ATGCAT 1 cut(s) 436
MroI TCCGGA 2 cut(s) 113, 2055
MroXI GAANNNNTTC 3 cut(s) 795, 1368, 2976
MslI CAYNNNNRTG 2 cut(s) 1106, 2924
MspA1I CMGCKG 3 cut(s) 174, 216, 2327
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 7 cut(s) 114, 242, 376, 479, 487, 2056, 2910
MspR9I CCNGG 9 cut(s) 90, 242, 488, 1329, 1432, 1983, 2602, 2911, 3033
MunI CAATTG 3 cut(s) 1590, 2169, 2319
Mva1269I GAATGC 1 cut(s) 2717
MvaI CCWGG 6 cut(s) 90, 1329, 1432, 1983, 2602, 3033
MvnI CGCG 6 cut(s) 65, 80, 174, 186, 216, 252
NciI CCSGG 3 cut(s) 242, 488, 2911
NcoI CCATGG 1 cut(s) 508
NheI GCTAGC 2 cut(s) 650, 1804
NlaIV GGNNCC 5 cut(s) 239, 240, 514, 2256, 2555
NmeAIII GCCGAG 3 cut(s) 167, 1213, 1846
NmuCI GTSAC 3 cut(s) 585, 1220, 2342
NotI GCGGCCGC 1 cut(s) 211
NsiI ATGCAT 1 cut(s) 436
OliI CACNNNNGTG 1 cut(s) 1106
PagI TCATGA 1 cut(s) 1177
PasI CCCWGGG 1 cut(s) 89
PauI GCGCGC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 2986
PctI GAATGC 1 cut(s) 2717
PdmI GAANNNNTTC 3 cut(s) 795, 1368, 2976
PfeI GAWTC 7 cut(s) 1024, 1061, 1451, 1692, 2147, 2411, 2977
PflMI CCANNNNNTGG 2 cut(s) 2264, 2638
PfoI TCCNGGA 1 cut(s) 1430
PleI GAGTC 2 cut(s) 573, 2840
PpsI GAGTC 2 cut(s) 573, 2840
PpuMI RGGWCCY 2 cut(s) 238, 2397
PshBI ATTAAT 1 cut(s) 1253
Psp5II RGGWCCY 2 cut(s) 238, 2397
Psp6I CCWGG 6 cut(s) 88, 1327, 1430, 1981, 2600, 3031
PspEI GGTNACC 1 cut(s) 2936
PspFI CCCAGC 4 cut(s) 1647, 2502, 2666, 2771
PspGI CCWGG 6 cut(s) 88, 1327, 1430, 1981, 2600, 3031
PspN4I GGNNCC 5 cut(s) 239, 240, 514, 2256, 2555
PspPI GGNCC 5 cut(s) 149, 238, 611, 2397, 2656
PspPPI RGGWCCY 2 cut(s) 238, 2397
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 9 cut(s) 93, 493, 921, 1050, 1243, 1462, 1702, 2254, 2553
PteI GCGCGC 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 2327
RsaI GTAC 3 cut(s) 1072, 2355, 3105
RsaNI GTAC 3 cut(s) 1071, 2354, 3104
RseI CAYNNNNRTG 2 cut(s) 1106, 2924
SacII CCGCGG 2 cut(s) 175, 217
Sau96I GGNCC 5 cut(s) 149, 238, 611, 2397, 2656
SchI GAGTC 2 cut(s) 574, 2840
ScrFI CCNGG 9 cut(s) 90, 242, 488, 1329, 1432, 1983, 2602, 2911, 3033
SduI GDGCHC 1 cut(s) 2673
SfcI CTRYAG 2 cut(s) 540, 672
Sfr303I CCGCGG 2 cut(s) 175, 217
SgrBI CCGCGG 2 cut(s) 175, 217
SinI GGWCC 3 cut(s) 238, 2397, 2656
SmiMI CAYNNNNRTG 2 cut(s) 1106, 2924
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SspI AATATT 2 cut(s) 2581, 3110
SspMI CTAG 5 cut(s) 651, 911, 1163, 1805, 2901
StyD4I CCNGG 9 cut(s) 88, 240, 486, 1327, 1430, 1981, 2600, 2909, 3031
StyI CCWWGG 4 cut(s) 19, 130, 508, 1696
TaaI ACNGT 4 cut(s) 1039, 1089, 1122, 1750
TaiI ACGT 1 cut(s) 931
TaqI TCGA 3 cut(s) 98, 787, 1117
TaqII GACCGA 1 cut(s) 317
TatI WGTACW 2 cut(s) 2353, 3103
TauI GCSGC 9 cut(s) 65, 150, 171, 174, 195, 198, 213, 216, 219
TfiI GAWTC 7 cut(s) 1024, 1061, 1451, 1692, 2147, 2411, 2977
TscAI CASTG 5 cut(s) 12, 613, 1108, 2425, 2696
TseFI GTSAC 3 cut(s) 585, 1220, 2342
TseI GCWGC 7 cut(s) 36, 268, 995, 2324, 2327, 2387, 3088
Tsp45I GTSAC 3 cut(s) 585, 1220, 2342
TspGWI ACGGA 2 cut(s) 351, 1043
TspRI CASTG 5 cut(s) 12, 613, 1108, 2425, 2696
Van91I CCANNNNNTGG 2 cut(s) 2264, 2638
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 3 cut(s) 238, 2397, 2656
VspI ATTAAT 1 cut(s) 1253
XagI CCTNNNNNAGG 1 cut(s) 1012
XapI RAATTY 5 cut(s) 390, 1173, 1306, 1426, 2854
XbaI TCTAGA 1 cut(s) 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1714
XmiI GTMKAC 1 cut(s) 539
XmnI GAANNNNTTC 3 cut(s) 795, 1368, 2976
XspI CTAG 5 cut(s) 651, 911, 1163, 1805, 2901
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.