Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

16665851 .. 16672494

Gene Structure 6644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G39930.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGATGCAATCAAATGCAGTTCCAGTTTCCTCCATCACACCAAGCTCAACACTCTCTTCTCTAATCACACCTTTCCTAAAATCTCCGCTCCCAATTTCAAGCCCCTCTTCCGTCCGATTTCGATTTCCGCAAAGGACAGAAGAAGCAACGAAGCTGAAAATATCGCCGTAGTAGAGAAACCTCTTAAATCAGATAGATTTTTTATCTCCGATGGACTTCCTTCTCCATTCGGACCCACCGTCAGAGACGACGGCGTCAATTTCTCTGTTTACTCTACAAACTCCGTTTCCGCTACCATCTGCTTGATTTCTCTCTCCGATCTCCGTCAGAACAAAGTGACGGAGGAGATTCAGCTTGATCCATCAAGGAATAGAACTGGCCATGTTTGGCATGTGTTCTTGAGAGGAGATTTCAAAGATATGTTGTATGGTTATAGATTTGATGGCAAGTTTTCTCCTGAAGAAGGTCATTATTATGATTCCTCCAACATTTTATTGGATCCTTACGCAAAGGCAATTATAAGCAGAGATGAGTTTGGAGTTTTGGGACCTGATGATAATTGTTGGCCTCAAATGGCGTGTATGGTACCCACTCGTGAGGAAGAGTTTGATTGGGAAGGGGATATGCATCTGAAGCTTCCACAGAAAGATCTTGTTATATATGAAATGCATGTGCGAGGTTTTACAAGGCATGAGTCTAGTAAAATTGAATTCCCTGGCACATACCAGGGTGTTGCAGAGAAGCTTGACCATTTGAAGGAGCTTGGGATAAATTGTATAGAATTAATGCCATGTCACGAGTTTAATGAGCTGGAGTATTACAGCTACAATACGATTTTGGGAGACCACAGGGTAAATTTTTGGGGTTACTCTACCATTGGGTTCTTCTCGCCCATGATCAGATACGCATCAGCAAGCTCTAACAATTTTGCTGGACGAGCCATAAATGAATTCAAAATTCTTGTTAAGGAGGCACATAAACGAGGAATTGAGGTAATCATGGATGTCGTCTTGAACCACACAGCCGAAGGGAATGAAAAAGGGCCCATTTTCTCATTTAGAGGAGTTGATAACAGTGTCTATTACATGCTTGCTCCAAAGGGCGAGTTCTATAATTATTCAGGCTGTGGTAATACATTCAACTGCAATCATCCTGTGGTGCGTCAATTCATATTGGATTGCCTGAGATATTGGGTTACAGAAATGCATGTTGACGGCTTCCGCTTTGATCTTGGTTCAATCATGTCAAGGAGCAGCAGCCTTTGGGATGCAGCCAATGTTTACGGGGCTGATGTAGAAGGTGACTTGCTCACAACTGGTACTCCTATTAGCTGCCCTCCAGTAATTGACATGATAAGTAATGATCCAATACTCCGCGGTGTTAAGCTAATAGCTGAAGCATGGGATGCGGGTGGGCTGTACCAAGTTGGCATGTTTCCACACTGGGGTATTTGGTCTGAGTGGAATGGAAAGTTTCGGGATGTTGTGAGACAGTTCATAAAAGGCACCGATGGCTTTTCTGGTGCTTTTGCTGAATGTCTCTGTGGAAGCCCAAATCTGTACCAGGGAGGTAGGAAACCTTGGCACAGCATCAATTTTATATGTGCGCATGATGGTTTTACGTTGGCAGATTTAGTAACTTACAACAATAAGAATAACTTGGCAAATGGAGAAGAGAATAATGATGGAGAGAATCACAATTACAGCTGGAACTGTGGAGAGGAGGGAGACTTTGCGAGTATCTCGGTGAAGAGACTAAGGAAACGACAGATGCGGAATTTCTTTGTTTCCCTCATGGTTTCCCAAGGTGTCCCAATGATTTACATGGGAGATGAATATGGCCATACTAAAGGGGGAAACAACAACACGTATTGCCATGACAACTATATGAACTATTTTCGGTGGGATAAAAAGGAAGAAGCACATTCTGACTTCTTCAGATTCTGCCGTATTCTTATCAAGTTTCGTGATGAATGTGAATCACTTGGCTTGAATGATTTCCCAACAGCAAAGCGTCTGCAGTGGCATGGTCTTGCTCCTGAGATCCCAAATTGGTCTGAAACAAGTCGATTTGTTGCATTTTCACTGGTCGATTCTGTGAAGAAAGAAATCTATGTGGCCTTCAACACCAGTCATTTAGCCACACTTGTTTCGCTACCGAATAGGCCAGGATACCGATGGGAGCCATTTGTAGACACGAGCAAACCGAGCCCTTACGACTGCATAACACCGGATCTCCCAGAGAGAGAAACGGCGATGAAGCAGTATAGGCACTTCTTAGATGCAAATGTGTACCCAATGCTCAGTTACTCATCCATCATTCTCCTTCTTTCACCAATTAAAGACCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

783

Amino Acids

89.48

Weight (kDa)

5.78

Isoelectric Point (pI)

43.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	76 - 170	1.9e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	230 - 344	3.7e-10	Alpha amylase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	521
AasI	GACNNNNNNGTC	1 cut(s)	254
Acc16I	TGCGCA	1 cut(s)	1608
Acc65I	GGTACC	1 cut(s)	586
AccB1I	GGYRCC	2 cut(s)	586, 1505
AccBSI	CCGCTC	1 cut(s)	89
AccI	GTMKAC	1 cut(s)	2193
AccII	CGCG	1 cut(s)	1377
AciI	CCGC	8 cut(s)	87, 129, 291, 1222, 1375, 1377, 1409, 1774
AclWI	GGATC	6 cut(s)	353, 494, 507, 1358, 2038, 2241
AcoI	YGGCCR	2 cut(s)	379, 1840
AcsI	RAATTY	5 cut(s)	710, 856, 950, 957, 1777
AcuI	CTGAAG	4 cut(s)	480, 653, 1416, 1921
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	5 cut(s)	588, 1321, 1421, 1562, 2294
AfiI	CCNNNNNNNGG	3 cut(s)	464, 757, 1445
AflIII	ACRYGT	1 cut(s)	1866
AhdI	GACNNNNNGTC	1 cut(s)	239
AjnI	CCWGG	4 cut(s)	715, 726, 1563, 2167
Alw26I	GTCTC	6 cut(s)	240, 837, 1483, 1544, 1722, 1747
AlwI	GGATC	6 cut(s)	353, 494, 507, 1358, 2038, 2241
AlwNI	CAGNNNCTG	1 cut(s)	1944
AoxI	GGCC	6 cut(s)	379, 566, 1043, 1840, 2118, 2165
ApaI	GGGCCC	1 cut(s)	1047
ApeKI	GCWGC	4 cut(s)	1254, 1257, 1271, 1332
ApoI	RAATTY	5 cut(s)	710, 856, 950, 957, 1777
AseI	ATTAAT	1 cut(s)	785
Asp700I	GAANNNNTTC	1 cut(s)	1997
Asp718I	GGTACC	1 cut(s)	586
AspLEI	GCGC	1 cut(s)	1609
AspS9I	GGNCC	4 cut(s)	233, 548, 1043, 1044
AsuHPI	GGTGA	3 cut(s)	1313, 1759, 2325
AvaII	GGWCC	2 cut(s)	233, 548
BaeGI	GKGCMC	1 cut(s)	1047
BalI	TGGCCA	2 cut(s)	381, 1842
BamHI	GGATCC	1 cut(s)	499
BanI	GGYRCC	2 cut(s)	586, 1505
BanII	GRGCYC	2 cut(s)	1047, 2213
BarI	GAAGNNNNNNTAC	2 cut(s)	2258, 2290
BauI	CACGAG	3 cut(s)	594, 797, 2197
BbvI	GCAGC	4 cut(s)	1266, 1269, 1283, 1319
BceAI	ACGGC	5 cut(s)	152, 268, 1231, 1932, 2268
BciT130I	CCWGG	4 cut(s)	717, 728, 1565, 2169
BciVI	GTATCC	1 cut(s)	2165
BclI	TGATCA	1 cut(s)	897
BcoDI	GTCTC	6 cut(s)	240, 837, 1483, 1544, 1722, 1747
BfaI	CTAG	1 cut(s)	699
BfmI	CTRYAG	1 cut(s)	2018
BfuI	GTATCC	1 cut(s)	2165
BglII	AGATCT	1 cut(s)	649
BisI	GCNGC	4 cut(s)	1255, 1258, 1272, 1333
BlsI	GCNGC	4 cut(s)	1256, 1259, 1273, 1334
Bme1390I	CCNGG	4 cut(s)	717, 728, 1565, 2169
Bme18I	GGWCC	2 cut(s)	233, 548
BmeRI	GACNNNNNGTC	1 cut(s)	239
BmgT120I	GGNCC	4 cut(s)	233, 548, 1043, 1044
BmiI	GGNNCC	7 cut(s)	235, 501, 549, 588, 1045, 1507, 2184
BmrFI	CCNGG	4 cut(s)	717, 728, 1565, 2169
BmrI	ACTGGG	1 cut(s)	1453
BmsI	GCATC	7 cut(s)	637, 917, 1258, 1396, 1599, 1761, 2272
BmuI	ACTGGG	1 cut(s)	1453
BpmI	CTGGAG	2 cut(s)	833, 1323
BpuEI	CTTGAG	1 cut(s)	421
BsaAI	YACGTR	1 cut(s)	1869
BsaBI	GATNNNNATC	2 cut(s)	627, 907
BsaHI	GRCGYC	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	837
BsaJI	CCNNGG	6 cut(s)	715, 727, 1375, 1564, 1580, 1804
BsaWI	WCCGGW	1 cut(s)	2230
BsaXI	ACNNNNNCTCC	4 cut(s)	1306, 1336, 2220, 2250
Bsc4I	CCNNNNNNNGG	3 cut(s)	464, 757, 1445
Bse1I	ACTGG	7 cut(s)	24, 382, 1321, 1340, 1448, 2091, 2130
Bse8I	GATNNNNATC	2 cut(s)	627, 907
BseBI	CCWGG	4 cut(s)	717, 728, 1565, 2169
BseDI	CCNNGG	6 cut(s)	715, 727, 1375, 1564, 1580, 1804
BseGI	GGATG	7 cut(s)	10, 1009, 1150, 1273, 1411, 1486, 2312
BseJI	GATNNNNATC	2 cut(s)	627, 907
BseLI	CCNNNNNNNGG	3 cut(s)	464, 757, 1445
BseMII	CTCAG	4 cut(s)	1175, 1449, 2031, 2317
BseNI	ACTGG	7 cut(s)	24, 382, 1321, 1340, 1448, 2091, 2130
BseRI	GAGGAG	4 cut(s)	359, 420, 1077, 1736
BseSI	GKGCMC	1 cut(s)	1047
BseXI	GCAGC	4 cut(s)	1266, 1269, 1283, 1319
Bsh1236I	CGCG	1 cut(s)	1377
BshFI	GGCC	6 cut(s)	381, 568, 1045, 1842, 2120, 2167
BshNI	GGYRCC	2 cut(s)	586, 1505
BsiSI	CCGG	1 cut(s)	2231
BslFI	GGGAC	2 cut(s)	561, 1796
BslI	CCNNNNNNNGG	3 cut(s)	464, 757, 1445
BsmAI	GTCTC	6 cut(s)	240, 837, 1483, 1544, 1722, 1747
BsmBI	CGTCTC	1 cut(s)	240
BsmFI	GGGAC	2 cut(s)	561, 1796
BsnI	GGCC	6 cut(s)	381, 568, 1045, 1842, 2120, 2167
Bso31I	GGTCTC	1 cut(s)	837
Bsp120I	GGGCCC	1 cut(s)	1043
Bsp1286I	GDGCHC	2 cut(s)	1047, 2213
Bsp143I	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
BspACI	CCGC	8 cut(s)	87, 129, 291, 1222, 1375, 1377, 1409, 1774
BspANI	GGCC	6 cut(s)	381, 568, 1045, 1842, 2120, 2167
BspCNI	CTCAG	4 cut(s)	1176, 1450, 2032, 2316
BspFNI	CGCG	1 cut(s)	1377
BspLI	GGNNCC	7 cut(s)	235, 501, 549, 588, 1045, 1507, 2184
BspMAI	CTGCAG	1 cut(s)	2022
BspPI	GGATC	6 cut(s)	353, 494, 507, 1358, 2038, 2241
BspT107I	GGYRCC	2 cut(s)	586, 1505
BspTNI	GGTCTC	1 cut(s)	837
BsrBI	CCGCTC	1 cut(s)	89
BsrI	ACTGG	7 cut(s)	24, 382, 1321, 1340, 1448, 2091, 2130
BssECI	CCNNGG	6 cut(s)	715, 727, 1375, 1564, 1580, 1804
BssMI	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	3 cut(s)	594, 797, 2197
BssT1I	CCWWGG	2 cut(s)	1580, 1804
Bst2BI	CACGAG	3 cut(s)	594, 797, 2197
Bst2UI	CCWGG	4 cut(s)	717, 728, 1565, 2169
Bst4CI	ACNGT	4 cut(s)	241, 1076, 1494, 1715
Bst6I	CTCTTC	5 cut(s)	62, 113, 597, 1668, 1745
BstACI	GRCGYC	1 cut(s)	255
BstAPI	GCANNNNNTGC	1 cut(s)	1406
BstBAI	YACGTR	1 cut(s)	1869
BstC8I	GCNNGC	2 cut(s)	916, 1092
BstDEI	CTNAG	6 cut(s)	1184, 1458, 1757, 2040, 2278, 2303
BstDSI	CCRYGG	1 cut(s)	1375
BstENI	CCTNNNNNAGG	1 cut(s)	462
BstF5I	GGATG	7 cut(s)	10, 1009, 1150, 1273, 1411, 1486, 2312
BstFNI	CGCG	1 cut(s)	1377
BstHHI	GCGC	1 cut(s)	1609
BstKTI	GATC	9 cut(s)	322, 361, 502, 652, 900, 1231, 1366, 2046, 2236
BstMAI	GTCTC	6 cut(s)	240, 837, 1483, 1544, 1722, 1747
BstMBI	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
BstMWI	GCNNNNNNNGC	6 cut(s)	634, 938, 1406, 1512, 2208, 2269
BstNI	CCWGG	4 cut(s)	717, 728, 1565, 2169
BstNSI	RCATGY	5 cut(s)	395, 674, 1090, 1211, 1435
BstSCI	CCNGG	4 cut(s)	715, 726, 1563, 2167
BstSFI	CTRYAG	1 cut(s)	2018
BstSLI	GKGCMC	1 cut(s)	1047
BstUI	CGCG	1 cut(s)	1377
BstV1I	GCAGC	4 cut(s)	1266, 1269, 1283, 1319
BstX2I	RGATCY	4 cut(s)	499, 649, 2043, 2233
BstYI	RGATCY	4 cut(s)	499, 649, 2043, 2233
BsuI	GTATCC	1 cut(s)	2165
BsuRI	GGCC	6 cut(s)	381, 568, 1045, 1842, 2120, 2167
BtgI	CCRYGG	1 cut(s)	1375
BtgZI	GCGATG	1 cut(s)	2270
BtsCI	GGATG	7 cut(s)	10, 1009, 1150, 1273, 1411, 1486, 2312
BtsI	GCAGTG	1 cut(s)	2027
BtsIMutI	CAGTG	4 cut(s)	1081, 1441, 2027, 2084
Cac8I	GCNNGC	2 cut(s)	916, 1092
CaiI	CAGNNNCTG	1 cut(s)	1944
CfoI	GCGC	1 cut(s)	1609
Cfr13I	GGNCC	4 cut(s)	233, 548, 1043, 1044
Cfr42I	CCGCGG	1 cut(s)	1378
CseI	GACGC	3 cut(s)	244, 1151, 2003
Csp6I	GTAC	5 cut(s)	587, 1320, 1420, 1561, 2293
CviQI	GTAC	5 cut(s)	587, 1320, 1420, 1561, 2293
DdeI	CTNAG	6 cut(s)	1184, 1458, 1757, 2040, 2278, 2303
DpnI	GATC	9 cut(s)	321, 360, 501, 651, 899, 1230, 1365, 2045, 2235
DpnII	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
DrdI	GACNNNNNNGTC	1 cut(s)	254
DriI	GACNNNNNGTC	1 cut(s)	239
DseDI	GACNNNNNNGTC	1 cut(s)	254
EaeI	YGGCCR	2 cut(s)	379, 1840
Eam1104I	CTCTTC	5 cut(s)	62, 113, 597, 1668, 1745
Eam1105I	GACNNNNNGTC	1 cut(s)	239
EarI	CTCTTC	5 cut(s)	62, 113, 597, 1668, 1745
Eco130I	CCWWGG	2 cut(s)	1580, 1804
Eco24I	GRGCYC	2 cut(s)	1047, 2213
Eco31I	GGTCTC	1 cut(s)	837
Eco47I	GGWCC	2 cut(s)	233, 548
Eco57I	CTGAAG	4 cut(s)	480, 653, 1416, 1921
EcoNI	CCTNNNNNAGG	1 cut(s)	462
EcoO109I	RGGNCCY	2 cut(s)	548, 1043
EcoRI	GAATTC	2 cut(s)	710, 950
EcoRII	CCWGG	4 cut(s)	715, 726, 1563, 2167
EcoT14I	CCWWGG	2 cut(s)	1580, 1804
EcoT22I	ATGCAT	3 cut(s)	630, 672, 1209
EcoT38I	GRGCYC	2 cut(s)	1047, 2213
ErhI	CCWWGG	2 cut(s)	1580, 1804
Esp3I	CGTCTC	1 cut(s)	240
FalI	AAGNNNNNCTT	4 cut(s)	92, 124, 1643, 1675
FaqI	GGGAC	2 cut(s)	561, 1796
FauI	CCCGC	1 cut(s)	1402
FbaI	TGATCA	1 cut(s)	897
FblI	GTMKAC	1 cut(s)	2193
Fnu4HI	GCNGC	4 cut(s)	1255, 1258, 1272, 1333
FokI	GGATG	7 cut(s)	17, 1016, 1137, 1280, 1418, 1493, 2299
FriOI	GRGCYC	2 cut(s)	1047, 2213
Fsp4HI	GCNGC	4 cut(s)	1255, 1258, 1272, 1333
FspAI	RTGCGCAY	1 cut(s)	1608
FspBI	CTAG	1 cut(s)	699
FspI	TGCGCA	1 cut(s)	1608
GlaI	GCGC	1 cut(s)	1608
GluI	GCNGC	4 cut(s)	1255, 1258, 1272, 1333
GsuI	CTGGAG	2 cut(s)	833, 1323
HaeIII	GGCC	6 cut(s)	381, 568, 1045, 1842, 2120, 2167
HapII	CCGG	1 cut(s)	2231
HgaI	GACGC	3 cut(s)	244, 1151, 2003
HhaI	GCGC	1 cut(s)	1609
Hin1I	GRCGYC	1 cut(s)	255
Hin6I	GCGC	1 cut(s)	1607
HinP1I	GCGC	1 cut(s)	1607
HincII	GTYRAC	1 cut(s)	1213
HindII	GTYRAC	1 cut(s)	1213
HindIII	AAGCTT	2 cut(s)	635, 743
HinfI	GANTC	7 cut(s)	349, 479, 695, 1693, 1941, 1979, 2093
HpaII	CCGG	1 cut(s)	2231
HphI	GGTGA	3 cut(s)	1313, 1759, 2325
Hpy166II	GTNNAC	5 cut(s)	271, 1213, 1282, 2194, 2293
Hpy188III	TCNNGA	8 cut(s)	400, 458, 596, 797, 1012, 1478, 1967, 2039
Hpy8I	GTNNAC	5 cut(s)	271, 1213, 1282, 2194, 2293
Hpy99I	CGWCG	1 cut(s)	254
HpyAV	CCTTC	8 cut(s)	231, 458, 611, 751, 1022, 1292, 2131, 2336
HpyCH4III	ACNGT	4 cut(s)	241, 1076, 1494, 1715
HpyCH4IV	ACGT	2 cut(s)	1622, 1868
HpyF10VI	GCNNNNNNNGC	6 cut(s)	634, 938, 1406, 1512, 2208, 2269
HpyF3I	CTNAG	6 cut(s)	1184, 1458, 1757, 2040, 2278, 2303
HpySE526I	ACGT	2 cut(s)	1622, 1868
Hsp92I	GRCGYC	1 cut(s)	255
HspAI	GCGC	1 cut(s)	1607
KpnI	GGTACC	1 cut(s)	590
Ksp22I	TGATCA	1 cut(s)	897
KspI	CCGCGG	1 cut(s)	1378
Kzo9I	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
LmnI	GCTCC	6 cut(s)	94, 760, 1099, 1251, 2041, 2182
Lsp1109I	GCAGC	4 cut(s)	1266, 1269, 1283, 1319
LweI	GCATC	7 cut(s)	637, 917, 1258, 1396, 1599, 1761, 2272
MaeI	CTAG	1 cut(s)	699
MaeII	ACGT	2 cut(s)	1622, 1868
MaeIII	GTNAC	7 cut(s)	337, 794, 866, 1195, 1301, 1636, 2306
MalI	GATC	9 cut(s)	321, 360, 501, 651, 899, 1230, 1365, 2045, 2235
MbiI	CCGCTC	1 cut(s)	89
MboI	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
MflI	RGATCY	4 cut(s)	499, 649, 2043, 2233
MhlI	GDGCHC	2 cut(s)	1047, 2213
MlsI	TGGCCA	2 cut(s)	381, 1842
MluNI	TGGCCA	2 cut(s)	381, 1842
MlyI	GAGTC	1 cut(s)	704
MmeI	TCCRAC	1 cut(s)	510
Mox20I	TGGCCA	2 cut(s)	381, 1842
Mph1103I	ATGCAT	3 cut(s)	630, 672, 1209
MroXI	GAANNNNTTC	1 cut(s)	1997
MscI	TGGCCA	2 cut(s)	381, 1842
MseI	TTAA	6 cut(s)	186, 785, 804, 966, 1383, 2340
MslI	CAYNNNNRTG	1 cut(s)	474
Msp20I	TGGCCA	2 cut(s)	381, 1842
MspA1I	CMGCKG	2 cut(s)	1377, 1707
MspI	CCGG	1 cut(s)	2231
MspR9I	CCNGG	4 cut(s)	717, 728, 1565, 2169
MvaI	CCWGG	4 cut(s)	717, 728, 1565, 2169
MvnI	CGCG	1 cut(s)	1377
MwoI	GCNNNNNNNGC	6 cut(s)	634, 938, 1406, 1512, 2208, 2269
NdeII	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
NlaIV	GGNNCC	7 cut(s)	235, 501, 549, 588, 1045, 1507, 2184
NmuCI	GTSAC	3 cut(s)	337, 794, 1301
NsbI	TGCGCA	1 cut(s)	1608
NsiI	ATGCAT	3 cut(s)	630, 672, 1209
NspI	RCATGY	5 cut(s)	395, 674, 1090, 1211, 1435
PcsI	WCGNNNNNNNCGW	2 cut(s)	237, 2204
PdmI	GAANNNNTTC	1 cut(s)	1997
PfeI	GAWTC	6 cut(s)	349, 479, 1693, 1941, 1979, 2093
PflFI	GACNNNGTC	1 cut(s)	254
PkrI	GCNGC	4 cut(s)	1256, 1259, 1273, 1334
PleI	GAGTC	1 cut(s)	703
PpsI	GAGTC	1 cut(s)	703
Ppu21I	YACGTR	1 cut(s)	1869
PpuMI	RGGWCCY	1 cut(s)	548
PshBI	ATTAAT	1 cut(s)	785
PsiI	TTATAA	1 cut(s)	521
Psp5II	RGGWCCY	1 cut(s)	548
Psp6I	CCWGG	4 cut(s)	715, 726, 1563, 2167
PspGI	CCWGG	4 cut(s)	715, 726, 1563, 2167
PspN4I	GGNNCC	7 cut(s)	235, 501, 549, 588, 1045, 1507, 2184
PspOMI	GGGCCC	1 cut(s)	1043
PspPI	GGNCC	4 cut(s)	233, 548, 1043, 1044
PspPPI	RGGWCCY	1 cut(s)	548
PstI	CTGCAG	1 cut(s)	2022
PstNI	CAGNNNCTG	1 cut(s)	1944
PsuI	RGATCY	4 cut(s)	499, 649, 2043, 2233
PsyI	GACNNNGTC	1 cut(s)	254
PvuII	CAGCTG	1 cut(s)	1707
RsaI	GTAC	5 cut(s)	588, 1321, 1421, 1562, 2294
RsaNI	GTAC	5 cut(s)	587, 1320, 1420, 1561, 2293
RseI	CAYNNNNRTG	1 cut(s)	474
SacII	CCGCGG	1 cut(s)	1378
SaqAI	TTAA	6 cut(s)	186, 785, 804, 966, 1383, 2340
SatI	GCNGC	4 cut(s)	1255, 1258, 1272, 1333
Sau3AI	GATC	9 cut(s)	319, 358, 499, 649, 897, 1228, 1363, 2043, 2233
Sau96I	GGNCC	4 cut(s)	233, 548, 1043, 1044
SchI	GAGTC	1 cut(s)	704
ScrFI	CCNGG	4 cut(s)	717, 728, 1565, 2169
SduI	GDGCHC	2 cut(s)	1047, 2213
SfaNI	GCATC	7 cut(s)	637, 917, 1258, 1396, 1599, 1761, 2272
SfcI	CTRYAG	1 cut(s)	2018
Sfr303I	CCGCGG	1 cut(s)	1378
SgrBI	CCGCGG	1 cut(s)	1378
SinI	GGWCC	2 cut(s)	233, 548
SmiMI	CAYNNNNRTG	1 cut(s)	474
SmlI	CTYRAG	1 cut(s)	400
SmoI	CTYRAG	1 cut(s)	400
SsiI	CCGC	8 cut(s)	87, 129, 291, 1222, 1375, 1377, 1409, 1774
SspMI	CTAG	1 cut(s)	699
StyD4I	CCNGG	4 cut(s)	715, 726, 1563, 2167
StyI	CCWWGG	2 cut(s)	1580, 1804
TaaI	ACNGT	4 cut(s)	241, 1076, 1494, 1715
TaiI	ACGT	2 cut(s)	1625, 1871
TaqI	TCGA	3 cut(s)	122, 2068, 2091
TfiI	GAWTC	6 cut(s)	349, 479, 1693, 1941, 1979, 2093
Tru1I	TTAA	6 cut(s)	186, 785, 804, 966, 1383, 2340
Tru9I	TTAA	6 cut(s)	186, 785, 804, 966, 1383, 2340
TscAI	CASTG	4 cut(s)	1081, 1448, 2027, 2091
TseFI	GTSAC	3 cut(s)	337, 794, 1301
TseI	GCWGC	4 cut(s)	1254, 1257, 1271, 1332
Tsp45I	GTSAC	3 cut(s)	337, 794, 1301
TspDTI	ATGAA	9 cut(s)	678, 963, 1050, 1159, 1486, 1848, 1904, 1986, 2273
TspGWI	ACGGA	4 cut(s)	101, 274, 314, 356
TspRI	CASTG	4 cut(s)	1081, 1448, 2027, 2091
Tth111I	GACNNNGTC	1 cut(s)	254
VpaK11BI	GGWCC	2 cut(s)	233, 548
VspI	ATTAAT	1 cut(s)	785
XagI	CCTNNNNNAGG	1 cut(s)	462
XapI	RAATTY	5 cut(s)	710, 856, 950, 957, 1777
XceI	RCATGY	5 cut(s)	395, 674, 1090, 1211, 1435
XcmI	CCANNNNNNNNNTGG	2 cut(s)	493, 2175
XmiI	GTMKAC	1 cut(s)	2193
XmnI	GAANNNNTTC	1 cut(s)	1997
XspI	CTAG	1 cut(s)	699
Zsp2I	ATGCAT	3 cut(s)	630, 672, 1209

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.