Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

11389572 .. 11399313

Gene Structure 9742 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG076700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGGAGCACCTAACCACTTGGTCCAGTGCTCCCAGACTCCACTCTCACCACCACCGCCTCCTCTTTGCTTCCAATGCAAGGCTCCGGAGCCGAAGCCCCAACCGTCTCAATTGCCGGATGATGCTAGGGTTTCGGAGAGGCGGTGCTCCGTCGGCGGTGAAGGCAAGCGACGGAGGCGGGGCGGTGGATACCGCGGTGGCCGAGAGGCCGAAACTGCGTCGTTTTCAGGTCTCCGAAGGTCATCCGACTCCCTTTGGCGCCACGCCCCGCGACGGTGGGGTTAACTTCGCTGTCTACGCCGGCAATGCCGTTTCCGCCACTCTCTGTTTGATCACTCTCGCCGATTTAGAGCAGGATAAAGTTACTGAGCAGATCCCTCTTGATCCTGTGAACAATAAGACTGGTAATGTGTGGCATGTATTTCTAACTGGAGATGTCAAAGATATCCTATATGGATACAAATTCGACGGCAAGTTTTCTCCGCAAGAGGGACACTACTATGATTCGTCAAAGATTGTATTGGATCCTTATGCAAAGGCAGTTATACGCAGAGGTGAATTTGGGAGTCTAGGTCCTGATGGTAATTGCTGGCCTCAAATGGCCTGCACGGTACCTTCTTTTGATGATAAGTTCAACTGGGAAGGAGATTTACCTCTGAAGTATCCACAAAAAGATCTTATCATATATGAAATGCATGTGCGAGGTTTTACAAGGCATGAATCAAGCATGACTGAATTGCCTGGTACATACCGTGGTTTGGTGGAGAAGCTTGATCACTTAAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCATGTCATGAGTTCAATGAACTGGAGTACTTTGGCTACAATTCTGTCTTGGGTGACTACAAGGTAAATTTTTGGGGATATTCAACTGTCAGTTACTTTTCACCAATGATAAGATATTCATCTGCTGGCACACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTGTTAAAGAAGCACATAGACGTGGAATTGAGGTGATCATGGATGTTGTTTTCAATCACACAGCTGAAGGGAATGAAAATGGTCCCATTTTGTCTTTTAGAGGTGCTGACAACAGTGTCTATTACATGCTCGCACCTAAGGGAGAGTTTTATAATTATTCAGGGTGTGGTAACACATTCAATTGCAACCATCCTGTTGTGCGACAATTCATAGTAGATTGCTTGAGATATTGGGTAACAGAGATGCACGTGGATGGGTTCCGCTTTGATCTTGCTTCTATTATGACCAGGGGTACCAGTCTCTGGGATTCAACTAATGTATACGGGAGTCTGTTAGAAGGTGACTTGTTGACAACTGGAACCCCTCTCGCTAGCCCTCCATTGATTGACATGATTAGTAATGATCCAATACTTCATGGAGTGAAGCTGATAGCTGAAGCTTGGGATGCAGGAGGCCTGTACCAACTTGGCATGTTTCCTCACTGGGGTATTTGGTCAGAATGGAATGGGAAGTATCGTGACACAGTGCGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTTTGGCCGAATGCCTTTGTGGGAGCCCTAATTTGTACCAGGAAGGGGGAGGGAGACCATGGAACAGTATCAATTTTATTTGTGCACACGATGGTTTTACTTTAGCTGATTTAGTAACCTATAACAACAAACATAACCTGGCAAACGGAGAAGGCAACAATGACGGAGAGAATCATAATAATAGCTGGAACTGCGGACAGGAGGGAGAATTCACAAGAATTTCAGTGAAAAAACTGAGGAAACGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTTTCCCAAGGTGTTCCAATGATATATATGGGTGATGAGTATGGTCATACAAAAGGGGGGAACAACAACACATATTGCCATGATAATTATATTAATTACTTCCGGTGGGATAAGAAGGAAGAGTCTTCATCAGATTTTTTCAGATTTTGCTGTCTTATGACCAAATTCCGCCAGGAATGTGAGTCACTTGGTTTGAATGACTTCCCAACAGCAGAGAGGCTGCAGTGGCATGGTCTTTCTCCTGGGCAGCCAGACTGGTCTAAAACAAGCCGCTTTGTGGCCTTTACTCTGGTTGACTCAGTGAAGAGAGAGATCTACATTGCCTTCAATGCCAGTCATTTACCAGTGAACATTTCACTGCCAGAGAGGCCTGGATACAGATGGGAACCCTTGGTAGACACCAGCAAGGCTTCACCCTTTGACTTCCTATCTAGTGACCTCCCACAAAGAGATATTGCAGTTAAACAGTATGCTCACTTTCTTGACGCCAATTTATACCCGATGCTAAGTTATTCTTCAGTCATCCTATTGCTCGCTCCAGTCGAAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

792

Amino Acids

89.4

Weight (kDa)

6.07

Isoelectric Point (pI)

40.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	84 - 178	2.1e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	242 - 352	9.6e-10	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	683 - 784	5.9e-46	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1137
AasI	GACNNNNNNGTC	1 cut(s)	1100
Acc65I	GGTACC	2 cut(s)	610, 1277
AccB1I	GGYRCC	3 cut(s)	257, 610, 1277
AccB7I	CCANNNNNTGG	1 cut(s)	1287
AccI	GTMKAC	3 cut(s)	294, 1305, 2220
AccII	CGCG	2 cut(s)	194, 270
AccIII	TCCGGA	1 cut(s)	84
AclWI	GGATC	6 cut(s)	367, 377, 518, 531, 800, 1382
AcoI	YGGCCR	3 cut(s)	198, 955, 1554
AcsI	RAATTY	7 cut(s)	461, 557, 880, 1757, 1767, 1804, 1988
AcuI	CTGAAG	4 cut(s)	677, 1071, 1440, 2325
AcvI	CACGTG	1 cut(s)	1234
AcyI	GRCGYC	2 cut(s)	258, 2310
AfaI	GTAC	7 cut(s)	612, 745, 842, 1279, 1445, 1531, 1586
AfiI	CCNNNNNNNGG	8 cut(s)	78, 272, 488, 757, 1287, 1469, 1594, 2101
AflIII	ACRYGT	1 cut(s)	944
AgsI	TTSAA	8 cut(s)	634, 829, 897, 1039, 1165, 1296, 2020, 2152
AjiI	CACGTC	1 cut(s)	1007
AjnI	CCWGG	7 cut(s)	739, 1271, 1587, 1686, 1995, 2065, 2194
AluBI	AGCT	7 cut(s)	769, 1049, 1411, 1418, 1424, 1655, 1734
AluI	AGCT	7 cut(s)	769, 1049, 1411, 1418, 1424, 1655, 1734
Alw21I	GWGCWC	4 cut(s)	9, 31, 148, 1636
Alw26I	GTCTC	4 cut(s)	110, 235, 1289, 1597
Alw44I	GTGCAC	1 cut(s)	1632
AlwI	GGATC	6 cut(s)	367, 377, 518, 531, 800, 1382
AlwNI	CAGNNNCTG	1 cut(s)	1287
Aor13HI	TCCGGA	1 cut(s)	84
AoxI	GGCC	9 cut(s)	198, 206, 590, 600, 955, 1438, 1554, 2103, 2192
ApaLI	GTGCAC	1 cut(s)	1632
ApeKI	GCWGC	2 cut(s)	2044, 2071
ApoI	RAATTY	7 cut(s)	461, 557, 880, 1757, 1767, 1804, 1988
AseI	ATTAAT	1 cut(s)	1917
Asp700I	GAANNNNTTC	1 cut(s)	2024
Asp718I	GGTACC	2 cut(s)	610, 1277
AspLEI	GCGC	1 cut(s)	260
AspS9I	GGNCC	3 cut(s)	21, 572, 1067
AsuHPI	GGTGA	9 cut(s)	38, 169, 566, 878, 906, 1030, 1337, 1868, 2229
AsuNHI	GCTAGC	1 cut(s)	1355
AvaII	GGWCC	3 cut(s)	21, 572, 1067
AxyI	CCTNAGG	1 cut(s)	1122
BaeGI	GKGCMC	1 cut(s)	1636
BaeI	ACNNNNGTAYC	2 cut(s)	180, 213
BamHI	GGATCC	1 cut(s)	523
BanI	GGYRCC	3 cut(s)	257, 610, 1277
BanII	GRGCYC	1 cut(s)	1577
BarI	GAAGNNNNNNTAC	2 cut(s)	633, 665
BbrPI	CACGTG	1 cut(s)	1234
BbsI	GAAGAC	1 cut(s)	1941
Bbv12I	GWGCWC	4 cut(s)	9, 31, 148, 1636
BbvI	GCAGC	2 cut(s)	2031, 2083
BccI	CCATC	6 cut(s)	572, 1182, 1232, 1529, 1634, 2199
BceAI	ACGGC	3 cut(s)	293, 484, 942
BciT130I	CCWGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
BciVI	GTATCC	4 cut(s)	181, 449, 672, 2192
BclI	TGATCA	3 cut(s)	330, 772, 1020
BcoDI	GTCTC	4 cut(s)	110, 235, 1289, 1597
BfaI	CTAG	4 cut(s)	125, 569, 1356, 2256
BfmI	CTRYAG	1 cut(s)	2045
BfoI	RGCGCY	1 cut(s)	261
BfuI	GTATCC	4 cut(s)	181, 449, 672, 2192
BglI	GCCNNNNNGGC	1 cut(s)	2191
BglII	AGATCT	2 cut(s)	673, 2136
BisI	GCNGC	4 cut(s)	1514, 2045, 2072, 2095
BlsI	GCNGC	4 cut(s)	1515, 2046, 2073, 2096
BmcAI	AGTACT	1 cut(s)	842
Bme1390I	CCNGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
Bme18I	GGWCC	3 cut(s)	21, 572, 1067
BmgBI	CACGTC	1 cut(s)	1007
BmgT120I	GGNCC	3 cut(s)	21, 572, 1067
BmrFI	CCNGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
BmrI	ACTGGG	2 cut(s)	646, 1477
BmsI	GCATC	6 cut(s)	111, 952, 1218, 1420, 2316, 2362
BmtI	GCTAGC	1 cut(s)	1359
BmuI	ACTGGG	2 cut(s)	646, 1477
BpiI	GAAGAC	1 cut(s)	1941
BpmI	CTGGAG	3 cut(s)	450, 857, 2346
BpuEI	CTTGAG	1 cut(s)	1228
BsaAI	YACGTR	2 cut(s)	947, 1234
BsaBI	GATNNNNATC	1 cut(s)	931
BsaHI	GRCGYC	2 cut(s)	258, 2310
BsaI	GGTCTC	2 cut(s)	235, 1597
BsaJI	CCNNGG	7 cut(s)	192, 751, 1272, 1607, 1831, 2066, 2214
BsaWI	WCCGGW	2 cut(s)	84, 1926
BsaXI	ACNNNNNCTCC	4 cut(s)	127, 157, 556, 586
Bsc4I	CCNNNNNNNGG	8 cut(s)	78, 272, 488, 757, 1287, 1469, 1594, 2101
Bse118I	RCCGGY	1 cut(s)	299
Bse21I	CCTNAGG	1 cut(s)	1122
Bse3DI	GCAATG	2 cut(s)	310, 2142
Bse8I	GATNNNNATC	1 cut(s)	931
BseAI	TCCGGA	1 cut(s)	84
BseBI	CCWGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
BseDI	CCNNGG	7 cut(s)	192, 751, 1272, 1607, 1831, 2066, 2214
BseGI	GGATG	7 cut(s)	123, 241, 1033, 1174, 1243, 1435, 2346
BseJI	GATNNNNATC	1 cut(s)	931
BseLI	CCNNNNNNNGG	8 cut(s)	78, 272, 488, 757, 1287, 1469, 1594, 2101
BseMI	GCAATG	2 cut(s)	310, 2142
BseMII	CTCAG	3 cut(s)	357, 1775, 2136
BseRI	GAGGAG	1 cut(s)	50
BseSI	GKGCMC	1 cut(s)	1636
BseXI	GCAGC	2 cut(s)	2031, 2083
BsgI	GTGCAG	1 cut(s)	589
Bsh1236I	CGCG	2 cut(s)	194, 270
BshFI	GGCC	9 cut(s)	200, 208, 592, 602, 957, 1440, 1556, 2105, 2194
BshNI	GGYRCC	3 cut(s)	257, 610, 1277
BsiHKAI	GWGCWC	4 cut(s)	9, 31, 148, 1636
BsiSI	CCGG	4 cut(s)	85, 115, 300, 1927
BslFI	GGGAC	2 cut(s)	504, 1053
BslI	CCNNNNNNNGG	8 cut(s)	78, 272, 488, 757, 1287, 1469, 1594, 2101
BsmAI	GTCTC	4 cut(s)	110, 235, 1289, 1597
BsmBI	CGTCTC	1 cut(s)	110
BsmFI	GGGAC	2 cut(s)	504, 1053
BsmI	GAATGC	1 cut(s)	1565
BsnI	GGCC	9 cut(s)	200, 208, 592, 602, 957, 1440, 1556, 2105, 2194
Bso31I	GGTCTC	2 cut(s)	235, 1597
Bsp1286I	GDGCHC	5 cut(s)	9, 31, 148, 1577, 1636
Bsp13I	TCCGGA	1 cut(s)	84
Bsp19I	CCATGG	1 cut(s)	1607
BspANI	GGCC	9 cut(s)	200, 208, 592, 602, 957, 1440, 1556, 2105, 2194
BspCNI	CTCAG	3 cut(s)	358, 1776, 2135
BspEI	TCCGGA	1 cut(s)	84
BspFNI	CGCG	2 cut(s)	194, 270
BspHI	TCATGA	1 cut(s)	820
BspMAI	CTGCAG	1 cut(s)	2049
BspOI	GCTAGC	1 cut(s)	1359
BspPI	GGATC	6 cut(s)	367, 377, 518, 531, 800, 1382
BspT107I	GGYRCC	3 cut(s)	257, 610, 1277
BspTNI	GGTCTC	2 cut(s)	235, 1597
BsrDI	GCAATG	2 cut(s)	310, 2142
BsrFI	RCCGGY	1 cut(s)	299
BssAI	RCCGGY	1 cut(s)	299
BssECI	CCNNGG	7 cut(s)	192, 751, 1272, 1607, 1831, 2066, 2214
BssNAI	GTATAC	1 cut(s)	1306
BssNI	GRCGYC	2 cut(s)	258, 2310
BssT1I	CCWWGG	3 cut(s)	1607, 1831, 2214
Bst1107I	GTATAC	1 cut(s)	1306
Bst2UI	CCWGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
Bst6I	CTCTTC	2 cut(s)	1938, 2123
BstACI	GRCGYC	2 cut(s)	258, 2310
BstBAI	YACGTR	2 cut(s)	947, 1234
BstC8I	GCNNGC	8 cut(s)	166, 301, 590, 604, 940, 1116, 1357, 2358
BstDEI	CTNAG	6 cut(s)	366, 1122, 1784, 2122, 2330, 2376
BstDSI	CCRYGG	3 cut(s)	192, 751, 1607
BstF5I	GGATG	7 cut(s)	123, 241, 1033, 1174, 1243, 1435, 2346
BstFNI	CGCG	2 cut(s)	194, 270
BstH2I	RGCGCY	1 cut(s)	261
BstHHI	GCGC	1 cut(s)	260
BstMAI	GTCTC	4 cut(s)	110, 235, 1289, 1597
BstNI	CCWGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
BstNSI	RCATGY	4 cut(s)	419, 698, 1114, 1459
BstSCI	CCNGG	7 cut(s)	739, 1271, 1587, 1686, 1995, 2065, 2194
BstSFI	CTRYAG	1 cut(s)	2045
BstSLI	GKGCMC	1 cut(s)	1636
BstUI	CGCG	2 cut(s)	194, 270
BstV1I	GCAGC	2 cut(s)	2031, 2083
BstV2I	GAAGAC	1 cut(s)	1941
BstX2I	RGATCY	4 cut(s)	372, 523, 673, 2136
BstYI	RGATCY	4 cut(s)	372, 523, 673, 2136
BstZ17I	GTATAC	1 cut(s)	1306
Bsu36I	CCTNAGG	1 cut(s)	1122
BsuI	GTATCC	4 cut(s)	181, 449, 672, 2192
BsuRI	GGCC	9 cut(s)	200, 208, 592, 602, 957, 1440, 1556, 2105, 2194
BtgI	CCRYGG	3 cut(s)	192, 751, 1607
BtrI	CACGTC	1 cut(s)	1007
BtsCI	GGATG	7 cut(s)	123, 241, 1033, 1174, 1243, 1435, 2346
BtsI	GCAGTG	2 cut(s)	2054, 2180
BtsIMutI	CAGTG	9 cut(s)	31, 1105, 1465, 1515, 1779, 2054, 2130, 2175, 2180
Cac8I	GCNNGC	8 cut(s)	166, 301, 590, 604, 940, 1116, 1357, 2358
CaiI	CAGNNNCTG	1 cut(s)	1287
CciI	TCATGA	1 cut(s)	820
CfoI	GCGC	1 cut(s)	260
Cfr10I	RCCGGY	1 cut(s)	299
Cfr13I	GGNCC	3 cut(s)	21, 572, 1067
Cfr42I	CCGCGG	1 cut(s)	195
CseI	GACGC	2 cut(s)	206, 2318
Csp6I	GTAC	7 cut(s)	611, 744, 841, 1278, 1444, 1530, 1585
CviQI	GTAC	7 cut(s)	611, 744, 841, 1278, 1444, 1530, 1585
DdeI	CTNAG	6 cut(s)	366, 1122, 1784, 2122, 2330, 2376
DinI	GGCGCC	1 cut(s)	259
DrdI	GACNNNNNNGTC	1 cut(s)	1100
DseDI	GACNNNNNNGTC	1 cut(s)	1100
EaeI	YGGCCR	3 cut(s)	198, 955, 1554
Eam1104I	CTCTTC	2 cut(s)	1938, 2123
EarI	CTCTTC	2 cut(s)	1938, 2123
EciI	GGCGGA	2 cut(s)	304, 1982
Eco130I	CCWWGG	3 cut(s)	1607, 1831, 2214
Eco147I	AGGCCT	2 cut(s)	1440, 2194
Eco24I	GRGCYC	1 cut(s)	1577
Eco31I	GGTCTC	2 cut(s)	235, 1597
Eco32I	GATATC	1 cut(s)	445
Eco47I	GGWCC	3 cut(s)	21, 572, 1067
Eco57I	CTGAAG	4 cut(s)	677, 1071, 1440, 2325
Eco72I	CACGTG	1 cut(s)	1234
Eco81I	CCTNAGG	1 cut(s)	1122
EcoO109I	RGGNCCY	1 cut(s)	572
EcoRI	GAATTC	1 cut(s)	1757
EcoRII	CCWGG	7 cut(s)	739, 1271, 1587, 1686, 1995, 2065, 2194
EcoRV	GATATC	1 cut(s)	445
EcoT14I	CCWWGG	3 cut(s)	1607, 1831, 2214
EcoT22I	ATGCAT	1 cut(s)	696
EcoT38I	GRGCYC	1 cut(s)	1577
EgeI	GGCGCC	1 cut(s)	259
EheI	GGCGCC	1 cut(s)	259
ErhI	CCWWGG	3 cut(s)	1607, 1831, 2214
Esp3I	CGTCTC	1 cut(s)	110
FalI	AAGNNNNNCTT	2 cut(s)	2323, 2355
FaqI	GGGAC	2 cut(s)	504, 1053
FauI	CCCGC	2 cut(s)	170, 275
FbaI	TGATCA	3 cut(s)	330, 772, 1020
FblI	GTMKAC	3 cut(s)	294, 1305, 2220
Fnu4HI	GCNGC	4 cut(s)	1514, 2045, 2072, 2095
FokI	GGATG	7 cut(s)	130, 228, 1040, 1161, 1250, 1442, 2333
FriOI	GRGCYC	1 cut(s)	1577
Fsp4HI	GCNGC	4 cut(s)	1514, 2045, 2072, 2095
FspBI	CTAG	4 cut(s)	125, 569, 1356, 2256
GlaI	GCGC	1 cut(s)	259
GluI	GCNGC	4 cut(s)	1514, 2045, 2072, 2095
GsuI	CTGGAG	3 cut(s)	450, 857, 2346
HaeII	RGCGCY	1 cut(s)	261
HaeIII	GGCC	9 cut(s)	200, 208, 592, 602, 957, 1440, 1556, 2105, 2194
HapII	CCGG	4 cut(s)	85, 115, 300, 1927
HgaI	GACGC	2 cut(s)	206, 2318
HhaI	GCGC	1 cut(s)	260
Hin1I	GRCGYC	2 cut(s)	258, 2310
Hin6I	GCGC	1 cut(s)	258
HinP1I	GCGC	1 cut(s)	258
HincII	GTYRAC	3 cut(s)	283, 1335, 2119
HindII	GTYRAC	3 cut(s)	283, 1335, 2119
HindIII	AAGCTT	2 cut(s)	767, 1422
HpaI	GTTAAC	1 cut(s)	283
HpaII	CCGG	4 cut(s)	85, 115, 300, 1927
HphI	GGTGA	9 cut(s)	38, 169, 566, 878, 906, 1030, 1337, 1868, 2229
Hpy166II	GTNNAC	9 cut(s)	283, 295, 391, 1306, 1335, 1634, 2119, 2173, 2221
Hpy188I	TCNGA	7 cut(s)	135, 235, 246, 657, 1483, 1957, 1967
Hpy188III	TCNNGA	6 cut(s)	85, 380, 575, 821, 1502, 2306
Hpy8I	GTNNAC	9 cut(s)	283, 295, 391, 1306, 1335, 1634, 2119, 2173, 2221
Hpy99I	CGWCG	5 cut(s)	154, 173, 222, 275, 470
HpyCH4IV	ACGT	3 cut(s)	946, 1006, 1233
HpyF3I	CTNAG	6 cut(s)	366, 1122, 1784, 2122, 2330, 2376
HpySE526I	ACGT	3 cut(s)	946, 1006, 1233
Hsp92I	GRCGYC	2 cut(s)	258, 2310
HspAI	GCGC	1 cut(s)	258
KasI	GGCGCC	1 cut(s)	257
Kpn2I	TCCGGA	1 cut(s)	84
KpnI	GGTACC	2 cut(s)	614, 1281
KroI	GCCGGC	1 cut(s)	299
KroNI	GCCGGC	1 cut(s)	301
Ksp22I	TGATCA	3 cut(s)	330, 772, 1020
KspAI	GTTAAC	1 cut(s)	283
KspI	CCGCGG	1 cut(s)	195
LmnI	GCTCC	7 cut(s)	4, 34, 87, 87, 151, 1572, 2365
Lsp1109I	GCAGC	2 cut(s)	2031, 2083
LweI	GCATC	6 cut(s)	111, 952, 1218, 1420, 2316, 2362
MaeI	CTAG	4 cut(s)	125, 569, 1356, 2256
MaeII	ACGT	3 cut(s)	946, 1006, 1233
MboII	GAAGA	4 cut(s)	1941, 1955, 2140, 2331
MfeI	CAATTG	2 cut(s)	109, 1165
MflI	RGATCY	4 cut(s)	372, 523, 673, 2136
MhlI	GDGCHC	5 cut(s)	9, 31, 148, 1577, 1636
Mly113I	GGCGCC	1 cut(s)	258
MlyI	GAGTC	7 cut(s)	30, 241, 574, 1321, 1955, 2015, 2114
MmeI	TCCRAC	1 cut(s)	269
Mph1103I	ATGCAT	1 cut(s)	696
MroI	TCCGGA	1 cut(s)	84
MroNI	GCCGGC	1 cut(s)	299
MroXI	GAANNNNTTC	1 cut(s)	2024
MseI	TTAA	6 cut(s)	282, 779, 809, 990, 1917, 2286
MslI	CAYNNNNRTG	3 cut(s)	498, 1005, 1236
MspA1I	CMGCKG	2 cut(s)	194, 1049
MspI	CCGG	4 cut(s)	85, 115, 300, 1927
MspR9I	CCNGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
MunI	CAATTG	2 cut(s)	109, 1165
Mva1269I	GAATGC	1 cut(s)	1565
MvaI	CCWGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
MvnI	CGCG	2 cut(s)	194, 270
NaeI	GCCGGC	1 cut(s)	301
NarI	GGCGCC	1 cut(s)	258
NcoI	CCATGG	1 cut(s)	1607
NgoMIV	GCCGGC	1 cut(s)	299
NheI	GCTAGC	1 cut(s)	1355
NmeAIII	GCCGAG	1 cut(s)	226
NmuCI	GTSAC	5 cut(s)	866, 1325, 1502, 2007, 2258
NsiI	ATGCAT	1 cut(s)	696
NspI	RCATGY	4 cut(s)	419, 698, 1114, 1459
PagI	TCATGA	1 cut(s)	820
PceI	AGGCCT	2 cut(s)	1440, 2194
PcsI	WCGNNNNNNNCGW	1 cut(s)	2364
PctI	GAATGC	1 cut(s)	1565
PdiI	GCCGGC	1 cut(s)	301
PdmI	GAANNNNTTC	1 cut(s)	2024
PfeI	GAWTC	4 cut(s)	503, 719, 1292, 1720
PflMI	CCANNNNNTGG	1 cut(s)	1287
PkrI	GCNGC	4 cut(s)	1515, 2046, 2073, 2096
PleI	GAGTC	7 cut(s)	30, 241, 573, 1320, 1954, 2014, 2114
PluTI	GGCGCC	1 cut(s)	261
PmaCI	CACGTG	1 cut(s)	1234
PmlI	CACGTG	1 cut(s)	1234
PpsI	GAGTC	7 cut(s)	30, 241, 573, 1320, 1954, 2014, 2114
Ppu21I	YACGTR	2 cut(s)	947, 1234
PpuMI	RGGWCCY	1 cut(s)	572
PshBI	ATTAAT	1 cut(s)	1917
PsiI	TTATAA	1 cut(s)	1137
Psp5II	RGGWCCY	1 cut(s)	572
Psp6I	CCWGG	7 cut(s)	739, 1271, 1587, 1686, 1995, 2065, 2194
PspCI	CACGTG	1 cut(s)	1234
PspGI	CCWGG	7 cut(s)	739, 1271, 1587, 1686, 1995, 2065, 2194
PspPI	GGNCC	3 cut(s)	21, 572, 1067
PspPPI	RGGWCCY	1 cut(s)	572
PstI	CTGCAG	1 cut(s)	2049
PstNI	CAGNNNCTG	1 cut(s)	1287
PsuI	RGATCY	4 cut(s)	372, 523, 673, 2136
PvuII	CAGCTG	1 cut(s)	1049
RsaI	GTAC	7 cut(s)	612, 745, 842, 1279, 1445, 1531, 1586
RsaNI	GTAC	7 cut(s)	611, 744, 841, 1278, 1444, 1530, 1585
RseI	CAYNNNNRTG	3 cut(s)	498, 1005, 1236
SacII	CCGCGG	1 cut(s)	195
SaqAI	TTAA	6 cut(s)	282, 779, 809, 990, 1917, 2286
SatI	GCNGC	4 cut(s)	1514, 2045, 2072, 2095
Sau96I	GGNCC	3 cut(s)	21, 572, 1067
ScaI	AGTACT	1 cut(s)	842
SchI	GAGTC	7 cut(s)	30, 241, 574, 1321, 1955, 2015, 2114
ScrFI	CCNGG	7 cut(s)	741, 1273, 1589, 1688, 1997, 2067, 2196
SduI	GDGCHC	5 cut(s)	9, 31, 148, 1577, 1636
SfaNI	GCATC	6 cut(s)	111, 952, 1218, 1420, 2316, 2362
SfcI	CTRYAG	1 cut(s)	2045
SfoI	GGCGCC	1 cut(s)	259
Sfr303I	CCGCGG	1 cut(s)	195
SgrBI	CCGCGG	1 cut(s)	195
SinI	GGWCC	3 cut(s)	21, 572, 1067
SmiMI	CAYNNNNRTG	3 cut(s)	498, 1005, 1236
SmlI	CTYRAG	1 cut(s)	1207
SmoI	CTYRAG	1 cut(s)	1207
SseBI	AGGCCT	2 cut(s)	1440, 2194
SspDI	GGCGCC	1 cut(s)	257
SspMI	CTAG	4 cut(s)	125, 569, 1356, 2256
StuI	AGGCCT	2 cut(s)	1440, 2194
StyD4I	CCNGG	7 cut(s)	739, 1271, 1587, 1686, 1995, 2065, 2194
StyI	CCWWGG	3 cut(s)	1607, 1831, 2214
TaiI	ACGT	3 cut(s)	949, 1009, 1236
TaqI	TCGA	2 cut(s)	465, 2367
TatI	WGTACW	1 cut(s)	840
TauI	GCSGC	2 cut(s)	1516, 2097
TfiI	GAWTC	4 cut(s)	503, 719, 1292, 1720
Tru1I	TTAA	6 cut(s)	282, 779, 809, 990, 1917, 2286
Tru9I	TTAA	6 cut(s)	282, 779, 809, 990, 1917, 2286
TscAI	CASTG	9 cut(s)	31, 1105, 1472, 1515, 1779, 2054, 2130, 2175, 2187
TseFI	GTSAC	5 cut(s)	866, 1325, 1502, 2007, 2258
TseI	GCWGC	2 cut(s)	2044, 2071
Tsp45I	GTSAC	5 cut(s)	866, 1325, 1502, 2007, 2258
TspDTI	ATGAA	9 cut(s)	702, 732, 846, 921, 987, 1074, 1183, 1388, 1941
TspGWI	ACGGA	4 cut(s)	138, 186, 1710, 1728
TspRI	CASTG	9 cut(s)	31, 1105, 1472, 1515, 1779, 2054, 2130, 2175, 2187
Van91I	CCANNNNNTGG	1 cut(s)	1287
VneI	GTGCAC	1 cut(s)	1632
VpaK11BI	GGWCC	3 cut(s)	21, 572, 1067
VspI	ATTAAT	1 cut(s)	1917
XapI	RAATTY	7 cut(s)	461, 557, 880, 1757, 1767, 1804, 1988
XceI	RCATGY	4 cut(s)	419, 698, 1114, 1459
XmiI	GTMKAC	3 cut(s)	294, 1305, 2220
XmnI	GAANNNNTTC	1 cut(s)	2024
XspI	CTAG	4 cut(s)	125, 569, 1356, 2256
ZrmI	AGTACT	1 cut(s)	842
Zsp2I	ATGCAT	1 cut(s)	696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.