Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

14276286 .. 14286174

Gene Structure 9889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG081600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGGAGCACCTAACCACTTGGTCCAGTGCTCTCAGACTCCGCTCTCACCGCCACCGCCTCTTTGCTTCCAATGCAAGGCTCCGGAGCCGAAGCCCCAACCGTCTCAATTGGCGGATGATGCTAGGGTTTCGGAGAGGCGGTGCTCCGTCGGCGGTGAAGGCAAGCGACGCAGGCGCGGCGGTGGTTACCGCGGTGGCCGAGAGGCCGAAACTGCGTCGTTTTCAGGTCTCCGAAGGTCATCCGACTCCCTTTGGCGCCACGCCCCGCGACGGTGGGGTTAACTTCGCTGTCTACGCCGGCAATGCCGTTTCCGCCACTCTCTGTTTGATCACTCTCGCCGATTTAGAGCAGGATAAAGTTACTGAGCAGATCCCTCTTGATCCTGTGAACAATAAGACTGGTAATGTGTGGCATGCATTTCTAACTGGAGATGTCAAAGATATCCTATATGGATACAAATTCGACGGCAAGTTTTCTCCGCAAGAGGGACACTACTATGATTCGTCAAAGATTGTATTGGATCCTTATGCAAAGGCAGTTATACGCAGAGGTGAATTTGGGAGTCTAGGTCCTGATGGTAATTGCTGGCCTCAAATGGCCTGCACGGTACCTTCTTTTGATGATAAGTTCAACTGGGAAGGAGATTTACCTCTGAAGTATCCACAAAAAGATCTTATCATATATGAAATGCACGTGCGAGGTTTTACAAGGCATGAATCAAGCATGACTGAATTGCCTGGTACATACCGTGGTTTGGTGGAGAAGCTTGATCACTTAAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCATGTCATGAGTTCAATGAACTGGAGTACTTTGGCTACAATTCTGTCTTGGGTGACTACAAGGTAAATTTTTGGGGATATTCAACTGTCAGTTACTTTTCACCAATGATAAGATATTCATCTGCTGGCACACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTGTTAAAGAAGCACATAGACGTGGAATTGAGGTGATCATGGATGTTGTTTTCAATCACACAGCTGAAGGGAATGAAAATGGTCCCATTTTGTCTTTTAGAGGTGCTGACAACAGTGTCTATTACATGCTCGCACCTAAGGGAGAGTTTTATAATTATTCAGGGTGTGGTAACACATTCAATTGCAACCATCCTGTTGTGCGACAATTCATAGTAGATTGCTTGAGATATTGGGTAACAGAGATGCACGTGGATGGGTTCCGCTTTGATCTTGCTTCTATTATGACCAGGGGTACCAGTCTCTGGGATTCAACTAATGTATACGGGAGTCTGATAGAAGGTGACTTGTTGACAACTGGAACCCCTCTCGCTAGCCCTCCATTGATTGACATGATTAGTAATGACCCAATACTTCATGGAGTGAAGCTGATAGCTGAAGCTTGGGATGGAGGAGGCCTGTACCAACTTGGCATGTTTCCTCACTGGGGTATTTGGTCAGAATGGAACGGGAAGTATCGTGACACAGTACGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTTTGGCCGAATGCCTTTGTGGGAGCCCTAATTTGTATCAGGAAGGGGGAGGGAGACCATGGAACAGTATCAATTTTATTTGTGCACACGATGGTTTTACTTTAGCTGATTTAGTAACCTATAACAACAAACATAACCTGGCAAACGGAGAAGACAACAATGACGGAGAGAATCATAATAATAGCTGGAACTGCGGACAGGAGGGAGAATTTGCAAGAATTTCAGTGAAAAAACTGAGGAAACGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTTTCCCAAGGTGTTCCAATGCTATATATGGGTGATGAGTATGGTCATACAAAAGGGGGGAACAACAACACATATTGCCATGATAATTATATTAATTACTTCCGGTGGGATAAGAAGGAAGAGTCTTCATCAGATTTTTTCAGATTTTGCTGTCTTATGACCAAATTCCGCCAGGAATGTGAGTCACTTGGTTTGAATGACTTCCCAACAGCAGAGAGGCTGCAGTGGCATGGTCTTTCTCCTGGGCAGCCAGACTGGTCTAAAACAAGCCGCTTTGTGGCCTTTACTCTGGTTGACTCAGTGAAGAGAGAGATCTACATTGCCTTCAATGCCAGTCATTTACCAGTGAACATTTCACTGCCAGAGAGGCCTGGATACAGATGGGAACCCTTGGTAGACACCAGCAAGGCTTCACCCTTTGACTTCCTATCTAGTGACCTCCCACAAAGAGATATTGCAGTTAAACAGTATGCTCACTTTCTTGACGCCAATTTATACCCGATGCTAAGTTATTCTTCAATCATCCTATTGCTCGCTCCAGTCGAAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

791

Amino Acids

89.44

Weight (kDa)

6.13

Isoelectric Point (pI)

42.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	83 - 177	2.6e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	241 - 351	9.6e-10	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	682 - 783	3.4e-46	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1134
AasI	GACNNNNNNGTC	1 cut(s)	1097
Acc65I	GGTACC	2 cut(s)	607, 1274
AccB1I	GGYRCC	3 cut(s)	254, 607, 1274
AccB7I	CCANNNNNTGG	1 cut(s)	1284
AccBSI	CCGCTC	1 cut(s)	42
AccI	GTMKAC	3 cut(s)	291, 1302, 2217
AccII	CGCG	3 cut(s)	176, 191, 267
AccIII	TCCGGA	1 cut(s)	81
AclWI	GGATC	5 cut(s)	364, 374, 515, 528, 797
AcoI	YGGCCR	3 cut(s)	195, 952, 1551
AcsI	RAATTY	7 cut(s)	458, 554, 877, 1754, 1764, 1801, 1985
AcuI	CTGAAG	3 cut(s)	674, 1068, 1437
AcvI	CACGTG	2 cut(s)	694, 1231
AcyI	GRCGYC	2 cut(s)	255, 2307
AfaI	GTAC	7 cut(s)	609, 742, 839, 1276, 1442, 1509, 1528
AfiI	CCNNNNNNNGG	7 cut(s)	75, 269, 485, 754, 1284, 1466, 2098
AflIII	ACRYGT	1 cut(s)	941
AgsI	TTSAA	9 cut(s)	631, 826, 894, 1036, 1162, 1293, 2017, 2149, 2340
AjiI	CACGTC	1 cut(s)	1004
AjnI	CCWGG	6 cut(s)	736, 1268, 1683, 1992, 2062, 2191
AluBI	AGCT	7 cut(s)	766, 1046, 1408, 1415, 1421, 1652, 1731
AluI	AGCT	7 cut(s)	766, 1046, 1408, 1415, 1421, 1652, 1731
Alw21I	GWGCWC	4 cut(s)	9, 31, 145, 1633
Alw26I	GTCTC	4 cut(s)	107, 232, 1286, 1594
Alw44I	GTGCAC	1 cut(s)	1629
AlwI	GGATC	5 cut(s)	364, 374, 515, 528, 797
AlwNI	CAGNNNCTG	1 cut(s)	1284
Aor13HI	TCCGGA	1 cut(s)	81
AoxI	GGCC	9 cut(s)	195, 203, 587, 597, 952, 1435, 1551, 2100, 2189
ApaLI	GTGCAC	1 cut(s)	1629
ApeKI	GCWGC	2 cut(s)	2041, 2068
ApoI	RAATTY	7 cut(s)	458, 554, 877, 1754, 1764, 1801, 1985
AseI	ATTAAT	1 cut(s)	1914
Asp700I	GAANNNNTTC	1 cut(s)	2021
Asp718I	GGTACC	2 cut(s)	607, 1274
AspLEI	GCGC	2 cut(s)	176, 257
AspS9I	GGNCC	3 cut(s)	21, 569, 1064
AsuHPI	GGTGA	9 cut(s)	38, 166, 563, 875, 903, 1027, 1334, 1865, 2226
AsuNHI	GCTAGC	1 cut(s)	1352
AvaII	GGWCC	3 cut(s)	21, 569, 1064
AxyI	CCTNAGG	1 cut(s)	1119
BaeGI	GKGCMC	1 cut(s)	1633
BamHI	GGATCC	1 cut(s)	520
BanI	GGYRCC	3 cut(s)	254, 607, 1274
BanII	GRGCYC	1 cut(s)	1574
BarI	GAAGNNNNNNTAC	2 cut(s)	630, 662
BbrPI	CACGTG	2 cut(s)	694, 1231
BbsI	GAAGAC	2 cut(s)	1704, 1938
Bbv12I	GWGCWC	4 cut(s)	9, 31, 145, 1633
BbvI	GCAGC	2 cut(s)	2028, 2080
BccI	CCATC	7 cut(s)	569, 1179, 1229, 1421, 1526, 1631, 2196
BceAI	ACGGC	4 cut(s)	290, 481, 939, 1526
BciT130I	CCWGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
BciVI	GTATCC	3 cut(s)	446, 669, 2189
BclI	TGATCA	3 cut(s)	327, 769, 1017
BcoDI	GTCTC	4 cut(s)	107, 232, 1286, 1594
BfaI	CTAG	4 cut(s)	122, 566, 1353, 2253
BfmI	CTRYAG	1 cut(s)	2042
BfoI	RGCGCY	1 cut(s)	258
BfuI	GTATCC	3 cut(s)	446, 669, 2189
BglI	GCCNNNNNGGC	1 cut(s)	2188
BglII	AGATCT	2 cut(s)	670, 2133
BisI	GCNGC	4 cut(s)	177, 2042, 2069, 2092
BlsI	GCNGC	4 cut(s)	178, 2043, 2070, 2093
BmcAI	AGTACT	1 cut(s)	839
Bme1390I	CCNGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
Bme18I	GGWCC	3 cut(s)	21, 569, 1064
BmgBI	CACGTC	1 cut(s)	1004
BmgT120I	GGNCC	3 cut(s)	21, 569, 1064
BmrFI	CCNGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
BmrI	ACTGGG	2 cut(s)	643, 1474
BmsI	GCATC	5 cut(s)	108, 949, 1215, 2313, 2359
BmtI	GCTAGC	1 cut(s)	1356
BmuI	ACTGGG	2 cut(s)	643, 1474
BpiI	GAAGAC	2 cut(s)	1704, 1938
BpmI	CTGGAG	3 cut(s)	447, 854, 2343
BpuEI	CTTGAG	1 cut(s)	1225
BsaAI	YACGTR	3 cut(s)	694, 944, 1231
BsaBI	GATNNNNATC	1 cut(s)	928
BsaHI	GRCGYC	2 cut(s)	255, 2307
BsaI	GGTCTC	2 cut(s)	232, 1594
BsaJI	CCNNGG	7 cut(s)	189, 748, 1269, 1604, 1828, 2063, 2211
BsaWI	WCCGGW	2 cut(s)	81, 1923
BsaXI	ACNNNNNCTCC	4 cut(s)	124, 154, 553, 583
Bsc4I	CCNNNNNNNGG	7 cut(s)	75, 269, 485, 754, 1284, 1466, 2098
Bse118I	RCCGGY	1 cut(s)	296
Bse21I	CCTNAGG	1 cut(s)	1119
Bse3DI	GCAATG	2 cut(s)	307, 2139
Bse8I	GATNNNNATC	1 cut(s)	928
BseAI	TCCGGA	1 cut(s)	81
BseBI	CCWGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
BseDI	CCNNGG	7 cut(s)	189, 748, 1269, 1604, 1828, 2063, 2211
BseGI	GGATG	7 cut(s)	120, 238, 1030, 1171, 1240, 1432, 2343
BseJI	GATNNNNATC	1 cut(s)	928
BseLI	CCNNNNNNNGG	7 cut(s)	75, 269, 485, 754, 1284, 1466, 2098
BseMI	GCAATG	2 cut(s)	307, 2139
BseMII	CTCAG	4 cut(s)	46, 354, 1772, 2133
BseRI	GAGGAG	1 cut(s)	1446
BseSI	GKGCMC	1 cut(s)	1633
BseXI	GCAGC	2 cut(s)	2028, 2080
BsgI	GTGCAG	1 cut(s)	586
Bsh1236I	CGCG	3 cut(s)	176, 191, 267
BshFI	GGCC	9 cut(s)	197, 205, 589, 599, 954, 1437, 1553, 2102, 2191
BshNI	GGYRCC	3 cut(s)	254, 607, 1274
BsiHKAI	GWGCWC	4 cut(s)	9, 31, 145, 1633
BsiSI	CCGG	3 cut(s)	82, 297, 1924
BslFI	GGGAC	2 cut(s)	501, 1050
BslI	CCNNNNNNNGG	7 cut(s)	75, 269, 485, 754, 1284, 1466, 2098
BsmAI	GTCTC	4 cut(s)	107, 232, 1286, 1594
BsmBI	CGTCTC	1 cut(s)	107
BsmFI	GGGAC	2 cut(s)	501, 1050
BsmI	GAATGC	1 cut(s)	1562
BsnI	GGCC	9 cut(s)	197, 205, 589, 599, 954, 1437, 1553, 2102, 2191
Bso31I	GGTCTC	2 cut(s)	232, 1594
Bsp1286I	GDGCHC	5 cut(s)	9, 31, 145, 1574, 1633
Bsp13I	TCCGGA	1 cut(s)	81
Bsp19I	CCATGG	1 cut(s)	1604
BspANI	GGCC	9 cut(s)	197, 205, 589, 599, 954, 1437, 1553, 2102, 2191
BspCNI	CTCAG	4 cut(s)	45, 355, 1773, 2132
BspEI	TCCGGA	1 cut(s)	81
BspFNI	CGCG	3 cut(s)	176, 191, 267
BspHI	TCATGA	1 cut(s)	817
BspMAI	CTGCAG	1 cut(s)	2046
BspOI	GCTAGC	1 cut(s)	1356
BspPI	GGATC	5 cut(s)	364, 374, 515, 528, 797
BspT107I	GGYRCC	3 cut(s)	254, 607, 1274
BspTNI	GGTCTC	2 cut(s)	232, 1594
BsrBI	CCGCTC	1 cut(s)	42
BsrDI	GCAATG	2 cut(s)	307, 2139
BsrFI	RCCGGY	1 cut(s)	296
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	7 cut(s)	189, 748, 1269, 1604, 1828, 2063, 2211
BssNAI	GTATAC	1 cut(s)	1303
BssNI	GRCGYC	2 cut(s)	255, 2307
BssT1I	CCWWGG	3 cut(s)	1604, 1828, 2211
Bst1107I	GTATAC	1 cut(s)	1303
Bst2UI	CCWGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
Bst6I	CTCTTC	2 cut(s)	1935, 2120
BstACI	GRCGYC	2 cut(s)	255, 2307
BstBAI	YACGTR	3 cut(s)	694, 944, 1231
BstDEI	CTNAG	7 cut(s)	32, 363, 1119, 1781, 2119, 2327, 2373
BstDSI	CCRYGG	3 cut(s)	189, 748, 1604
BstEII	GGTNACC	1 cut(s)	184
BstF5I	GGATG	7 cut(s)	120, 238, 1030, 1171, 1240, 1432, 2343
BstFNI	CGCG	3 cut(s)	176, 191, 267
BstH2I	RGCGCY	1 cut(s)	258
BstHHI	GCGC	2 cut(s)	176, 257
BstMAI	GTCTC	4 cut(s)	107, 232, 1286, 1594
BstNI	CCWGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
BstNSI	RCATGY	3 cut(s)	416, 1111, 1456
BstPI	GGTNACC	1 cut(s)	184
BstSCI	CCNGG	6 cut(s)	736, 1268, 1683, 1992, 2062, 2191
BstSFI	CTRYAG	1 cut(s)	2042
BstSLI	GKGCMC	1 cut(s)	1633
BstUI	CGCG	3 cut(s)	176, 191, 267
BstV1I	GCAGC	2 cut(s)	2028, 2080
BstV2I	GAAGAC	2 cut(s)	1704, 1938
BstX2I	RGATCY	4 cut(s)	369, 520, 670, 2133
BstYI	RGATCY	4 cut(s)	369, 520, 670, 2133
BstZ17I	GTATAC	1 cut(s)	1303
Bsu36I	CCTNAGG	1 cut(s)	1119
BsuI	GTATCC	3 cut(s)	446, 669, 2189
BsuRI	GGCC	9 cut(s)	197, 205, 589, 599, 954, 1437, 1553, 2102, 2191
BtgI	CCRYGG	3 cut(s)	189, 748, 1604
BtrI	CACGTC	1 cut(s)	1004
BtsCI	GGATG	7 cut(s)	120, 238, 1030, 1171, 1240, 1432, 2343
BtsI	GCAGTG	2 cut(s)	2051, 2177
BtsIMutI	CAGTG	8 cut(s)	31, 1102, 1462, 1776, 2051, 2127, 2172, 2177
CaiI	CAGNNNCTG	1 cut(s)	1284
CciI	TCATGA	1 cut(s)	817
CfoI	GCGC	2 cut(s)	176, 257
Cfr10I	RCCGGY	1 cut(s)	296
Cfr13I	GGNCC	3 cut(s)	21, 569, 1064
Cfr42I	CCGCGG	1 cut(s)	192
CseI	GACGC	3 cut(s)	176, 203, 2315
Csp6I	GTAC	7 cut(s)	608, 741, 838, 1275, 1441, 1508, 1527
CviQI	GTAC	7 cut(s)	608, 741, 838, 1275, 1441, 1508, 1527
DdeI	CTNAG	7 cut(s)	32, 363, 1119, 1781, 2119, 2327, 2373
DinI	GGCGCC	1 cut(s)	256
DrdI	GACNNNNNNGTC	1 cut(s)	1097
DseDI	GACNNNNNNGTC	1 cut(s)	1097
EaeI	YGGCCR	3 cut(s)	195, 952, 1551
Eam1104I	CTCTTC	2 cut(s)	1935, 2120
EarI	CTCTTC	2 cut(s)	1935, 2120
EciI	GGCGGA	3 cut(s)	127, 301, 1979
Eco130I	CCWWGG	3 cut(s)	1604, 1828, 2211
Eco147I	AGGCCT	2 cut(s)	1437, 2191
Eco24I	GRGCYC	1 cut(s)	1574
Eco31I	GGTCTC	2 cut(s)	232, 1594
Eco32I	GATATC	1 cut(s)	442
Eco47I	GGWCC	3 cut(s)	21, 569, 1064
Eco57I	CTGAAG	3 cut(s)	674, 1068, 1437
Eco72I	CACGTG	2 cut(s)	694, 1231
Eco81I	CCTNAGG	1 cut(s)	1119
Eco91I	GGTNACC	1 cut(s)	184
EcoO109I	RGGNCCY	1 cut(s)	569
EcoO65I	GGTNACC	1 cut(s)	184
EcoRII	CCWGG	6 cut(s)	736, 1268, 1683, 1992, 2062, 2191
EcoRV	GATATC	1 cut(s)	442
EcoT14I	CCWWGG	3 cut(s)	1604, 1828, 2211
EcoT22I	ATGCAT	1 cut(s)	418
EcoT38I	GRGCYC	1 cut(s)	1574
EgeI	GGCGCC	1 cut(s)	256
EheI	GGCGCC	1 cut(s)	256
ErhI	CCWWGG	3 cut(s)	1604, 1828, 2211
Esp3I	CGTCTC	1 cut(s)	107
FalI	AAGNNNNNCTT	2 cut(s)	2320, 2352
FaqI	GGGAC	2 cut(s)	501, 1050
FauI	CCCGC	1 cut(s)	272
FbaI	TGATCA	3 cut(s)	327, 769, 1017
FblI	GTMKAC	3 cut(s)	291, 1302, 2217
Fnu4HI	GCNGC	4 cut(s)	177, 2042, 2069, 2092
FokI	GGATG	7 cut(s)	127, 225, 1037, 1158, 1247, 1439, 2330
FriOI	GRGCYC	1 cut(s)	1574
Fsp4HI	GCNGC	4 cut(s)	177, 2042, 2069, 2092
FspBI	CTAG	4 cut(s)	122, 566, 1353, 2253
GlaI	GCGC	2 cut(s)	175, 256
GluI	GCNGC	4 cut(s)	177, 2042, 2069, 2092
GsuI	CTGGAG	3 cut(s)	447, 854, 2343
HaeII	RGCGCY	1 cut(s)	258
HaeIII	GGCC	9 cut(s)	197, 205, 589, 599, 954, 1437, 1553, 2102, 2191
HapII	CCGG	3 cut(s)	82, 297, 1924
HgaI	GACGC	3 cut(s)	176, 203, 2315
HhaI	GCGC	2 cut(s)	176, 257
Hin1I	GRCGYC	2 cut(s)	255, 2307
Hin6I	GCGC	2 cut(s)	174, 255
HinP1I	GCGC	2 cut(s)	174, 255
HincII	GTYRAC	3 cut(s)	280, 1332, 2116
HindII	GTYRAC	3 cut(s)	280, 1332, 2116
HindIII	AAGCTT	2 cut(s)	764, 1419
HpaI	GTTAAC	1 cut(s)	280
HpaII	CCGG	3 cut(s)	82, 297, 1924
HphI	GGTGA	9 cut(s)	38, 166, 563, 875, 903, 1027, 1334, 1865, 2226
Hpy166II	GTNNAC	9 cut(s)	280, 292, 388, 1303, 1332, 1631, 2116, 2170, 2218
Hpy188I	TCNGA	9 cut(s)	35, 132, 232, 243, 654, 1314, 1480, 1954, 1964
Hpy188III	TCNNGA	7 cut(s)	82, 377, 572, 818, 1499, 1586, 2303
Hpy8I	GTNNAC	9 cut(s)	280, 292, 388, 1303, 1332, 1631, 2116, 2170, 2218
Hpy99I	CGWCG	5 cut(s)	151, 170, 219, 272, 467
HpyAV	CCTTC	9 cut(s)	151, 227, 621, 632, 1043, 1313, 1583, 1930, 2155
HpyCH4IV	ACGT	4 cut(s)	693, 943, 1003, 1230
HpyF3I	CTNAG	7 cut(s)	32, 363, 1119, 1781, 2119, 2327, 2373
HpySE526I	ACGT	4 cut(s)	693, 943, 1003, 1230
Hsp92I	GRCGYC	2 cut(s)	255, 2307
HspAI	GCGC	2 cut(s)	174, 255
KasI	GGCGCC	1 cut(s)	254
Kpn2I	TCCGGA	1 cut(s)	81
KpnI	GGTACC	2 cut(s)	611, 1278
KroI	GCCGGC	1 cut(s)	296
KroNI	GCCGGC	1 cut(s)	298
Ksp22I	TGATCA	3 cut(s)	327, 769, 1017
KspAI	GTTAAC	1 cut(s)	280
KspI	CCGCGG	1 cut(s)	192
LmnI	GCTCC	6 cut(s)	4, 84, 84, 148, 1569, 2362
Lsp1109I	GCAGC	2 cut(s)	2028, 2080
LweI	GCATC	5 cut(s)	108, 949, 1215, 2313, 2359
MaeI	CTAG	4 cut(s)	122, 566, 1353, 2253
MaeII	ACGT	4 cut(s)	693, 943, 1003, 1230
MbiI	CCGCTC	1 cut(s)	42
MboII	GAAGA	5 cut(s)	1709, 1938, 1952, 2137, 2328
MfeI	CAATTG	2 cut(s)	106, 1162
MflI	RGATCY	4 cut(s)	369, 520, 670, 2133
MhlI	GDGCHC	5 cut(s)	9, 31, 145, 1574, 1633
Mly113I	GGCGCC	1 cut(s)	255
MlyI	GAGTC	7 cut(s)	30, 238, 571, 1318, 1952, 2012, 2111
MmeI	TCCRAC	1 cut(s)	266
Mph1103I	ATGCAT	1 cut(s)	418
MroI	TCCGGA	1 cut(s)	81
MroNI	GCCGGC	1 cut(s)	296
MroXI	GAANNNNTTC	1 cut(s)	2021
MseI	TTAA	6 cut(s)	279, 776, 806, 987, 1914, 2283
MslI	CAYNNNNRTG	3 cut(s)	495, 1002, 1233
MspA1I	CMGCKG	2 cut(s)	191, 1046
MspI	CCGG	3 cut(s)	82, 297, 1924
MspR9I	CCNGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
MunI	CAATTG	2 cut(s)	106, 1162
Mva1269I	GAATGC	1 cut(s)	1562
MvaI	CCWGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
MvnI	CGCG	3 cut(s)	176, 191, 267
NaeI	GCCGGC	1 cut(s)	298
NarI	GGCGCC	1 cut(s)	255
NcoI	CCATGG	1 cut(s)	1604
NgoMIV	GCCGGC	1 cut(s)	296
NheI	GCTAGC	1 cut(s)	1352
NmeAIII	GCCGAG	1 cut(s)	223
NmuCI	GTSAC	5 cut(s)	863, 1322, 1499, 2004, 2255
NsiI	ATGCAT	1 cut(s)	418
NspI	RCATGY	3 cut(s)	416, 1111, 1456
PaeI	GCATGC	1 cut(s)	416
PagI	TCATGA	1 cut(s)	817
PceI	AGGCCT	2 cut(s)	1437, 2191
PcsI	WCGNNNNNNNCGW	1 cut(s)	2361
PctI	GAATGC	1 cut(s)	1562
PdiI	GCCGGC	1 cut(s)	298
PdmI	GAANNNNTTC	1 cut(s)	2021
PfeI	GAWTC	4 cut(s)	500, 716, 1289, 1717
PflMI	CCANNNNNTGG	1 cut(s)	1284
PkrI	GCNGC	4 cut(s)	178, 2043, 2070, 2093
PleI	GAGTC	7 cut(s)	30, 238, 570, 1317, 1951, 2011, 2111
PluTI	GGCGCC	1 cut(s)	258
PmaCI	CACGTG	2 cut(s)	694, 1231
PmlI	CACGTG	2 cut(s)	694, 1231
PpsI	GAGTC	7 cut(s)	30, 238, 570, 1317, 1951, 2011, 2111
Ppu21I	YACGTR	3 cut(s)	694, 944, 1231
PpuMI	RGGWCCY	1 cut(s)	569
PshBI	ATTAAT	1 cut(s)	1914
PsiI	TTATAA	1 cut(s)	1134
Psp5II	RGGWCCY	1 cut(s)	569
Psp6I	CCWGG	6 cut(s)	736, 1268, 1683, 1992, 2062, 2191
PspCI	CACGTG	2 cut(s)	694, 1231
PspEI	GGTNACC	1 cut(s)	184
PspGI	CCWGG	6 cut(s)	736, 1268, 1683, 1992, 2062, 2191
PspPI	GGNCC	3 cut(s)	21, 569, 1064
PspPPI	RGGWCCY	1 cut(s)	569
PstI	CTGCAG	1 cut(s)	2046
PstNI	CAGNNNCTG	1 cut(s)	1284
PsuI	RGATCY	4 cut(s)	369, 520, 670, 2133
PvuII	CAGCTG	1 cut(s)	1046
RsaI	GTAC	7 cut(s)	609, 742, 839, 1276, 1442, 1509, 1528
RsaNI	GTAC	7 cut(s)	608, 741, 838, 1275, 1441, 1508, 1527
RseI	CAYNNNNRTG	3 cut(s)	495, 1002, 1233
SacII	CCGCGG	1 cut(s)	192
SaqAI	TTAA	6 cut(s)	279, 776, 806, 987, 1914, 2283
SatI	GCNGC	4 cut(s)	177, 2042, 2069, 2092
Sau96I	GGNCC	3 cut(s)	21, 569, 1064
ScaI	AGTACT	1 cut(s)	839
SchI	GAGTC	7 cut(s)	30, 238, 571, 1318, 1952, 2012, 2111
ScrFI	CCNGG	6 cut(s)	738, 1270, 1685, 1994, 2064, 2193
SduI	GDGCHC	5 cut(s)	9, 31, 145, 1574, 1633
SfaNI	GCATC	5 cut(s)	108, 949, 1215, 2313, 2359
SfcI	CTRYAG	1 cut(s)	2042
SfoI	GGCGCC	1 cut(s)	256
Sfr303I	CCGCGG	1 cut(s)	192
SgrBI	CCGCGG	1 cut(s)	192
SinI	GGWCC	3 cut(s)	21, 569, 1064
SmiMI	CAYNNNNRTG	3 cut(s)	495, 1002, 1233
SmlI	CTYRAG	1 cut(s)	1204
SmoI	CTYRAG	1 cut(s)	1204
SphI	GCATGC	1 cut(s)	416
SseBI	AGGCCT	2 cut(s)	1437, 2191
SspDI	GGCGCC	1 cut(s)	254
SspMI	CTAG	4 cut(s)	122, 566, 1353, 2253
StuI	AGGCCT	2 cut(s)	1437, 2191
StyD4I	CCNGG	6 cut(s)	736, 1268, 1683, 1992, 2062, 2191
StyI	CCWWGG	3 cut(s)	1604, 1828, 2211
TaiI	ACGT	4 cut(s)	696, 946, 1006, 1233
TaqI	TCGA	2 cut(s)	462, 2364
TatI	WGTACW	1 cut(s)	837
TauI	GCSGC	2 cut(s)	179, 2094
TfiI	GAWTC	4 cut(s)	500, 716, 1289, 1717
Tru1I	TTAA	6 cut(s)	279, 776, 806, 987, 1914, 2283
Tru9I	TTAA	6 cut(s)	279, 776, 806, 987, 1914, 2283
TscAI	CASTG	8 cut(s)	31, 1102, 1469, 1776, 2051, 2127, 2172, 2184
TseFI	GTSAC	5 cut(s)	863, 1322, 1499, 2004, 2255
TseI	GCWGC	2 cut(s)	2041, 2068
Tsp45I	GTSAC	5 cut(s)	863, 1322, 1499, 2004, 2255
TspDTI	ATGAA	9 cut(s)	699, 729, 843, 918, 984, 1071, 1180, 1385, 1938
TspGWI	ACGGA	3 cut(s)	135, 1707, 1725
TspRI	CASTG	8 cut(s)	31, 1102, 1469, 1776, 2051, 2127, 2172, 2184
Van91I	CCANNNNNTGG	1 cut(s)	1284
VneI	GTGCAC	1 cut(s)	1629
VpaK11BI	GGWCC	3 cut(s)	21, 569, 1064
VspI	ATTAAT	1 cut(s)	1914
XapI	RAATTY	7 cut(s)	458, 554, 877, 1754, 1764, 1801, 1985
XceI	RCATGY	3 cut(s)	416, 1111, 1456
XmiI	GTMKAC	3 cut(s)	291, 1302, 2217
XmnI	GAANNNNTTC	1 cut(s)	2021
XspI	CTAG	4 cut(s)	122, 566, 1353, 2253
ZrmI	AGTACT	1 cut(s)	839
Zsp2I	ATGCAT	1 cut(s)	418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.