Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

26071084 .. 26075895

Gene Structure 4812 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1241000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 873 bp

ATGATTGTTGCGGCAGTTTATCAAAGGTACAGTGGCTTTTCTGGGGCTTTGGCTGAATGCCTTTGTGGGAGCCCTAATTTATATCAGGAAGGAGGGAGGCCATGGAATAGTATCAATTTTGTTTGTGCACACGATAGTTTTACTTTAGCTGATTTAGTAATCTATAACACCAAACATAACCTGGCAAATGGAGAAGACAACAATGATGGAGAGAGTCATAATAATAGCTGGAACTGTGGACAGGAGGGAGAGTTAACAAGCATTTCAGTGAAGAAATTGAGGAAACGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTTTCCCAAGGTGTCCCGATGCTGTGTATGGGTGATGAATATGGTCATACAAAAGGGGGAAACAACAACAAATATTGCCATGATAATTATATTAATTACTTTCGATGGGATAAGAAAGAAGAGTCCTCATCGGACTTTTTCAGATTTTGCTACCTCATGACCAAATTCCGCCAGGAATGCAAGTCACTTGGGTTAAATGACTTCCCAACGGCAGAGAGGCTGCAGTGGCATGGTCATGCTCCTGGGGTGCCAGACTGGTCTGACACAAGCCGCTTTGTTGCCTTTACTCTGATGGACGCTGTGAAGAGAGAGCTGTATATCGCTTTCAATGCCAGCCACTTAGCTGTGATCATTTCTCTGCCGGAGAGGCCTGGATACAGATGGGAGCCCTTGGTCGACACCAGCAAGCCTGCACCATTTGACTTCCTTACTAGTGACCTTCCAGAAAGAGATACCGCAATTAAACAGTATGCTCACTTTCTCGACGCCAATTTATATCCCATGCTAAGTTATTCTTCAATCATCCTGTTACTTTCTCCCGTCGAAGATCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

291

Amino Acids

33.08

Weight (kDa)

5.71

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ISOA1-3_C	PF21156	181 - 282	2.9e-48	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	565
AccI	GTMKAC	1 cut(s)	714
AciI	CCGC	5 cut(s)	11, 295, 487, 589, 774
AclWI	GGATC	1 cut(s)	860
AcsI	RAATTY	2 cut(s)	298, 482
AcyI	GRCGYC	1 cut(s)	804
AfaI	GTAC	1 cut(s)	29
AgsI	TTSAA	2 cut(s)	646, 837
AhlI	ACTAGT	1 cut(s)	749
AjnI	CCWGG	4 cut(s)	180, 489, 559, 688
AluBI	AGCT	4 cut(s)	149, 228, 631, 662
AluI	AGCT	4 cut(s)	149, 228, 631, 662
Alw21I	GWGCWC	1 cut(s)	130
Alw44I	GTGCAC	1 cut(s)	126
AlwI	GGATC	1 cut(s)	860
AoxI	GGCC	2 cut(s)	98, 686
ApaLI	GTGCAC	1 cut(s)	126
ApeKI	GCWGC	1 cut(s)	538
ApoI	RAATTY	2 cut(s)	298, 482
AseI	ATTAAT	1 cut(s)	411
AsuHPI	GGTGA	1 cut(s)	362
BaeGI	GKGCMC	1 cut(s)	130
BanI	GGYRCC	1 cut(s)	565
BanII	GRGCYC	2 cut(s)	74, 708
BbsI	GAAGAC	1 cut(s)	201
Bbv12I	GWGCWC	1 cut(s)	130
BbvI	GCAGC	1 cut(s)	525
BccI	CCATC	4 cut(s)	200, 417, 604, 693
BceAI	ACGGC	1 cut(s)	543
BciT130I	CCWGG	4 cut(s)	182, 491, 561, 690
BciVI	GTATCC	1 cut(s)	686
BclI	TGATCA	1 cut(s)	666
BcuI	ACTAGT	1 cut(s)	749
BfaI	CTAG	1 cut(s)	750
BfmI	CTRYAG	1 cut(s)	539
BfuI	GTATCC	1 cut(s)	686
BglI	GCCNNNNNGGC	1 cut(s)	685
BisI	GCNGC	3 cut(s)	12, 539, 589
BlsI	GCNGC	3 cut(s)	13, 540, 590
Bme1390I	CCNGG	4 cut(s)	182, 491, 561, 690
BmiI	GGNNCC	3 cut(s)	71, 567, 705
BmrFI	CCNGG	4 cut(s)	182, 491, 561, 690
BmsI	GCATC	1 cut(s)	327
BpiI	GAAGAC	1 cut(s)	201
BsaHI	GRCGYC	1 cut(s)	804
BsaJI	CCNNGG	4 cut(s)	101, 325, 560, 708
BsaXI	ACNNNNNCTCC	2 cut(s)	236, 266
Bse1I	ACTGG	1 cut(s)	578
BseBI	CCWGG	4 cut(s)	182, 491, 561, 690
BseDI	CCNNGG	4 cut(s)	101, 325, 560, 708
BseGI	GGATG	1 cut(s)	840
BseNI	ACTGG	1 cut(s)	578
BseSI	GKGCMC	1 cut(s)	130
BseXI	GCAGC	1 cut(s)	525
BsgI	GTGCAG	1 cut(s)	714
BshFI	GGCC	2 cut(s)	100, 688
BshNI	GGYRCC	1 cut(s)	565
BsiHKAI	GWGCWC	1 cut(s)	130
BsiSI	CCGG	1 cut(s)	680
BslFI	GGGAC	1 cut(s)	317
BsmFI	GGGAC	1 cut(s)	317
BsmI	GAATGC	2 cut(s)	62, 500
BsnI	GGCC	2 cut(s)	100, 688
Bsp1286I	GDGCHC	3 cut(s)	74, 130, 708
Bsp143I	GATC	2 cut(s)	666, 865
Bsp19I	CCATGG	1 cut(s)	101
BspACI	CCGC	5 cut(s)	11, 295, 487, 589, 774
BspANI	GGCC	2 cut(s)	100, 688
BspHI	TCATGA	1 cut(s)	474
BspLI	GGNNCC	3 cut(s)	71, 567, 705
BspMAI	CTGCAG	1 cut(s)	543
BspPI	GGATC	1 cut(s)	860
BspT107I	GGYRCC	1 cut(s)	565
BsrI	ACTGG	1 cut(s)	578
BssECI	CCNNGG	4 cut(s)	101, 325, 560, 708
BssMI	GATC	2 cut(s)	666, 865
BssNI	GRCGYC	1 cut(s)	804
BssT1I	CCWWGG	3 cut(s)	101, 325, 708
Bst2UI	CCWGG	4 cut(s)	182, 491, 561, 690
Bst4CI	ACNGT	3 cut(s)	32, 236, 786
Bst6I	CTCTTC	2 cut(s)	432, 617
BstACI	GRCGYC	1 cut(s)	804
BstC8I	GCNNGC	3 cut(s)	652, 725, 729
BstDEI	CTNAG	2 cut(s)	658, 824
BstDSI	CCRYGG	1 cut(s)	101
BstF5I	GGATG	1 cut(s)	840
BstKTI	GATC	2 cut(s)	669, 868
BstMBI	GATC	2 cut(s)	666, 865
BstMWI	GCNNNNNNNGC	4 cut(s)	495, 544, 647, 685
BstNI	CCWGG	4 cut(s)	182, 491, 561, 690
BstSCI	CCNGG	4 cut(s)	180, 489, 559, 688
BstSFI	CTRYAG	1 cut(s)	539
BstSLI	GKGCMC	1 cut(s)	130
BstV1I	GCAGC	1 cut(s)	525
BstV2I	GAAGAC	1 cut(s)	201
BstX2I	RGATCY	1 cut(s)	865
BstYI	RGATCY	1 cut(s)	865
BsuI	GTATCC	1 cut(s)	686
BsuRI	GGCC	2 cut(s)	100, 688
BtgI	CCRYGG	1 cut(s)	101
BtsCI	GGATG	1 cut(s)	840
BtsI	GCAGTG	1 cut(s)	548
BtsIMutI	CAGTG	3 cut(s)	37, 273, 548
Cac8I	GCNNGC	3 cut(s)	652, 725, 729
CciI	TCATGA	1 cut(s)	474
CseI	GACGC	2 cut(s)	623, 812
Csp6I	GTAC	1 cut(s)	28
CviAII	CATG	7 cut(s)	102, 316, 398, 475, 548, 554, 820
CviQI	GTAC	1 cut(s)	28
DdeI	CTNAG	2 cut(s)	658, 824
DpnI	GATC	2 cut(s)	668, 867
DpnII	GATC	2 cut(s)	666, 865
Eam1104I	CTCTTC	2 cut(s)	432, 617
EarI	CTCTTC	2 cut(s)	432, 617
EciI	GGCGGA	1 cut(s)	476
Eco130I	CCWWGG	3 cut(s)	101, 325, 708
Eco147I	AGGCCT	1 cut(s)	688
Eco24I	GRGCYC	2 cut(s)	74, 708
EcoRII	CCWGG	4 cut(s)	180, 489, 559, 688
EcoT14I	CCWWGG	3 cut(s)	101, 325, 708
EcoT38I	GRGCYC	2 cut(s)	74, 708
ErhI	CCWWGG	3 cut(s)	101, 325, 708
FaeI	CATG	7 cut(s)	105, 319, 401, 478, 551, 557, 823
FalI	AAGNNNNNCTT	2 cut(s)	817, 849
FaqI	GGGAC	1 cut(s)	317
FatI	CATG	7 cut(s)	101, 315, 397, 474, 547, 553, 819
FbaI	TGATCA	1 cut(s)	666
FblI	GTMKAC	1 cut(s)	714
Fnu4HI	GCNGC	3 cut(s)	12, 539, 589
FokI	GGATG	1 cut(s)	827
FriOI	GRGCYC	2 cut(s)	74, 708
Fsp4HI	GCNGC	3 cut(s)	12, 539, 589
FspBI	CTAG	1 cut(s)	750
GluI	GCNGC	3 cut(s)	12, 539, 589
HaeIII	GGCC	2 cut(s)	100, 688
HapII	CCGG	1 cut(s)	680
HgaI	GACGC	2 cut(s)	623, 812
Hin1I	GRCGYC	1 cut(s)	804
Hin1II	CATG	7 cut(s)	105, 319, 401, 478, 551, 557, 823
HincII	GTYRAC	2 cut(s)	255, 715
HindII	GTYRAC	2 cut(s)	255, 715
HinfI	GANTC	2 cut(s)	214, 440
HpaI	GTTAAC	1 cut(s)	255
HpaII	CCGG	1 cut(s)	680
HphI	GGTGA	1 cut(s)	362
Hpy166II	GTNNAC	4 cut(s)	128, 239, 255, 715
Hpy188I	TCNGA	4 cut(s)	451, 461, 580, 609
Hpy188III	TCNNGA	5 cut(s)	86, 334, 475, 761, 800
Hpy8I	GTNNAC	4 cut(s)	128, 239, 255, 715
Hpy99I	CGWCG	2 cut(s)	806, 863
HpyAV	CCTTC	2 cut(s)	83, 767
HpyCH4III	ACNGT	3 cut(s)	32, 236, 786
HpyCH4V	TGCA	4 cut(s)	128, 498, 541, 731
HpyF10VI	GCNNNNNNNGC	4 cut(s)	495, 544, 647, 685
HpyF3I	CTNAG	2 cut(s)	658, 824
Hsp92I	GRCGYC	1 cut(s)	804
Hsp92II	CATG	7 cut(s)	105, 319, 401, 478, 551, 557, 823
Ksp22I	TGATCA	1 cut(s)	666
KspAI	GTTAAC	1 cut(s)	255
Kzo9I	GATC	2 cut(s)	666, 865
LmnI	GCTCC	3 cut(s)	69, 562, 703
Lsp1109I	GCAGC	1 cut(s)	525
LweI	GCATC	1 cut(s)	327
MaeI	CTAG	1 cut(s)	750
MaeIII	GTNAC	3 cut(s)	501, 752, 846
MalI	GATC	2 cut(s)	668, 867
MboI	GATC	2 cut(s)	666, 865
MboII	GAAGA	5 cut(s)	206, 283, 449, 634, 825
MflI	RGATCY	1 cut(s)	865
MhlI	GDGCHC	3 cut(s)	74, 130, 708
MluCI	AATT	9 cut(s)	76, 115, 275, 298, 403, 412, 482, 777, 808
MlyI	GAGTC	2 cut(s)	223, 449
MnlI	CCTC	9 cut(s)	86, 90, 238, 273, 323, 454, 482, 528, 678
MseI	TTAA	4 cut(s)	254, 411, 512, 780
MslI	CAYNNNNRTG	2 cut(s)	266, 552
MspI	CCGG	1 cut(s)	680
MspR9I	CCNGG	4 cut(s)	182, 491, 561, 690
Mva1269I	GAATGC	2 cut(s)	62, 500
MvaI	CCWGG	4 cut(s)	182, 491, 561, 690
MwoI	GCNNNNNNNGC	4 cut(s)	495, 544, 647, 685
NcoI	CCATGG	1 cut(s)	101
NdeII	GATC	2 cut(s)	666, 865
NlaIII	CATG	7 cut(s)	105, 319, 401, 478, 551, 557, 823
NlaIV	GGNNCC	3 cut(s)	71, 567, 705
NmuCI	GTSAC	2 cut(s)	501, 752
PagI	TCATGA	1 cut(s)	474
PceI	AGGCCT	1 cut(s)	688
PctI	GAATGC	2 cut(s)	62, 500
PkrI	GCNGC	3 cut(s)	13, 540, 590
PleI	GAGTC	2 cut(s)	222, 448
PpsI	GAGTC	2 cut(s)	222, 448
PshBI	ATTAAT	1 cut(s)	411
Psp6I	CCWGG	4 cut(s)	180, 489, 559, 688
PspGI	CCWGG	4 cut(s)	180, 489, 559, 688
PspN4I	GGNNCC	3 cut(s)	71, 567, 705
PstI	CTGCAG	1 cut(s)	543
PsuI	RGATCY	1 cut(s)	865
RsaI	GTAC	1 cut(s)	29
RsaNI	GTAC	1 cut(s)	28
RseI	CAYNNNNRTG	2 cut(s)	266, 552
SalI	GTCGAC	1 cut(s)	713
SaqAI	TTAA	4 cut(s)	254, 411, 512, 780
SatI	GCNGC	3 cut(s)	12, 539, 589
Sau3AI	GATC	2 cut(s)	666, 865
SchI	GAGTC	2 cut(s)	223, 449
ScrFI	CCNGG	4 cut(s)	182, 491, 561, 690
SduI	GDGCHC	3 cut(s)	74, 130, 708
SetI	ASST	9 cut(s)	29, 151, 183, 230, 331, 474, 633, 664, 759
SfaNI	GCATC	1 cut(s)	327
SfcI	CTRYAG	1 cut(s)	539
SmiMI	CAYNNNNRTG	2 cut(s)	266, 552
SpeI	ACTAGT	1 cut(s)	749
Sse9I	AATT	9 cut(s)	76, 115, 275, 298, 403, 412, 482, 777, 808
SseBI	AGGCCT	1 cut(s)	688
SsiI	CCGC	5 cut(s)	11, 295, 487, 589, 774
SspI	AATATT	1 cut(s)	392
SspMI	CTAG	1 cut(s)	750
StuI	AGGCCT	1 cut(s)	688
StyD4I	CCNGG	4 cut(s)	180, 489, 559, 688
StyI	CCWWGG	3 cut(s)	101, 325, 708
TaaI	ACNGT	3 cut(s)	32, 236, 786
TaqI	TCGA	4 cut(s)	421, 714, 801, 861
TasI	AATT	9 cut(s)	76, 115, 275, 298, 403, 412, 482, 777, 808
TauI	GCSGC	2 cut(s)	14, 591
Tru1I	TTAA	4 cut(s)	254, 411, 512, 780
Tru9I	TTAA	4 cut(s)	254, 411, 512, 780
TscAI	CASTG	3 cut(s)	37, 273, 548
TseFI	GTSAC	2 cut(s)	501, 752
TseI	GCWGC	1 cut(s)	538
Tsp45I	GTSAC	2 cut(s)	501, 752
TspDTI	ATGAA	1 cut(s)	369
TspRI	CASTG	3 cut(s)	37, 273, 548
VneI	GTGCAC	1 cut(s)	126
VspI	ATTAAT	1 cut(s)	411
XapI	RAATTY	2 cut(s)	298, 482
XcmI	CCANNNNNNNNNTGG	1 cut(s)	178
XmiI	GTMKAC	1 cut(s)	714
XspI	CTAG	1 cut(s)	750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.