Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

16545979 .. 16548710

Gene Structure 2732 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG091500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 822 bp

ATGGAGCACCTAACCACTTGGTCCAGTGCTCCCAGACTCCACTCTCACCACCACCGCCTCTTTGCTTCCAATGCAAGGCTCCGGAGCCGAAGCGCCAACCGTCTCAATTGGCGAATGCTAGGGCTTCGGAGGGGCGGTGCTCCGTCGGCGGTGAAGGCAAGCGACGGAGGCGCGGCGGTGGATACAGCGGTGGCCGAGAGGCCGAAACTGCGTCGTTTTCAGGTCTCCGAAGGTCATCCGACTCCCTTTGGCGCCACGCTCCGCGACGGTGGGGTTAACTTCGCTGTCTACGCCGGCAATGCCGTTTCCGCCACTCTCTGTTTGATCACTCTCGCCGATTTAGAGCAGGATAAAGTTACTGAGCTGATCCCTCTTGATCCTGTGAACAATAAGACTGGTAATGTGTGGCATGTATTTCTAACTGGAGATCTCAAAGATATCCTGTATGGATACAAATTCGACGGCAAGTATTCTCCGCAAGAGGGACACTACTATGATTCGTCAAAGATTGTATTGGATCCTTATGCAAAGGCAGTTATACGCAGAGGTGAATTTGGGAGTCTAGGTCCTGATGGTAATTGCTGGCCTCAAATGGCCTGCACGGTACCTTCTTTTGATGATAAGTTCAACTGGGAAGGAGATTTACCTCTGAAGTATCCACAAAAAGATCTTATCATATATGAAATGCATGTGCGAGGTTTTACAAGGCATGAATCGAGCATGACTGAATTGCCTGGTACATACTGTGGTTTGGTGGAGAAGCTTGATCACTTAAAGGTACTCTTCCGAGATCTTGAAGAGAAAAATGCCCTCCTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

273

Amino Acids

30.53

Weight (kDa)

7.74

Isoelectric Point (pI)

46.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	82 - 176	1.7e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	604
AccB1I	GGYRCC	2 cut(s)	251, 604
AccI	GTMKAC	1 cut(s)	288
AccII	CGCG	2 cut(s)	173, 264
AccIII	TCCGGA	1 cut(s)	81
AciI	CCGC	9 cut(s)	55, 135, 149, 173, 176, 188, 262, 309, 476
AclWI	GGATC	4 cut(s)	361, 371, 512, 525
AcoI	YGGCCR	1 cut(s)	192
AcsI	RAATTY	2 cut(s)	455, 551
AcuI	CTGAAG	1 cut(s)	671
AcyI	GRCGYC	1 cut(s)	252
AfaI	GTAC	3 cut(s)	606, 739, 780
AfiI	CCNNNNNNNGG	2 cut(s)	75, 482
AgsI	TTSAA	2 cut(s)	628, 797
AjnI	CCWGG	1 cut(s)	733
AluBI	AGCT	2 cut(s)	364, 763
AluI	AGCT	2 cut(s)	364, 763
Alw21I	GWGCWC	3 cut(s)	9, 31, 142
Alw26I	GTCTC	2 cut(s)	107, 229
AlwI	GGATC	4 cut(s)	361, 371, 512, 525
Aor13HI	TCCGGA	1 cut(s)	81
AoxI	GGCC	4 cut(s)	192, 200, 584, 594
ApoI	RAATTY	2 cut(s)	455, 551
Asp718I	GGTACC	1 cut(s)	604
AspLEI	GCGC	3 cut(s)	95, 173, 254
AspS9I	GGNCC	2 cut(s)	21, 566
AsuHPI	GGTGA	3 cut(s)	38, 163, 560
AvaII	GGWCC	2 cut(s)	21, 566
BaeI	ACNNNNGTAYC	2 cut(s)	174, 207
BamHI	GGATCC	1 cut(s)	517
BanI	GGYRCC	2 cut(s)	251, 604
BarI	GAAGNNNNNNTAC	2 cut(s)	627, 659
Bbv12I	GWGCWC	3 cut(s)	9, 31, 142
BccI	CCATC	1 cut(s)	566
BceAI	ACGGC	2 cut(s)	287, 478
BciT130I	CCWGG	1 cut(s)	735
BciVI	GTATCC	3 cut(s)	175, 443, 666
BclI	TGATCA	2 cut(s)	324, 766
BcoDI	GTCTC	2 cut(s)	107, 229
BfaI	CTAG	2 cut(s)	119, 563
BfoI	RGCGCY	2 cut(s)	96, 255
BfuI	GTATCC	3 cut(s)	175, 443, 666
BglII	AGATCT	3 cut(s)	427, 667, 790
BisI	GCNGC	1 cut(s)	174
BlsI	GCNGC	1 cut(s)	175
Bme1390I	CCNGG	1 cut(s)	735
Bme18I	GGWCC	2 cut(s)	21, 566
BmgT120I	GGNCC	2 cut(s)	21, 566
BmiI	GGNNCC	5 cut(s)	80, 86, 253, 519, 606
BmrFI	CCNGG	1 cut(s)	735
BmrI	ACTGGG	1 cut(s)	640
BmuI	ACTGGG	1 cut(s)	640
BpmI	CTGGAG	1 cut(s)	444
BsaHI	GRCGYC	1 cut(s)	252
BsaI	GGTCTC	1 cut(s)	229
BsaWI	WCCGGW	1 cut(s)	81
BsaXI	ACNNNNNCTCC	4 cut(s)	121, 151, 550, 580
Bsc4I	CCNNNNNNNGG	2 cut(s)	75, 482
Bse118I	RCCGGY	1 cut(s)	293
Bse1I	ACTGG	4 cut(s)	24, 400, 427, 635
Bse3DI	GCAATG	1 cut(s)	304
BseAI	TCCGGA	1 cut(s)	81
BseBI	CCWGG	1 cut(s)	735
BseGI	GGATG	1 cut(s)	235
BseLI	CCNNNNNNNGG	2 cut(s)	75, 482
BseMI	GCAATG	1 cut(s)	304
BseMII	CTCAG	1 cut(s)	351
BseNI	ACTGG	4 cut(s)	24, 400, 427, 635
BsgI	GTGCAG	1 cut(s)	583
Bsh1236I	CGCG	2 cut(s)	173, 264
BshFI	GGCC	4 cut(s)	194, 202, 586, 596
BshNI	GGYRCC	2 cut(s)	251, 604
BsiHKAI	GWGCWC	3 cut(s)	9, 31, 142
BsiSI	CCGG	2 cut(s)	82, 294
BslFI	GGGAC	1 cut(s)	498
BslI	CCNNNNNNNGG	2 cut(s)	75, 482
BsmAI	GTCTC	2 cut(s)	107, 229
BsmBI	CGTCTC	1 cut(s)	107
BsmFI	GGGAC	1 cut(s)	498
BsmI	GAATGC	1 cut(s)	120
BsnI	GGCC	4 cut(s)	194, 202, 586, 596
Bso31I	GGTCTC	1 cut(s)	229
Bsp1286I	GDGCHC	3 cut(s)	9, 31, 142
Bsp13I	TCCGGA	1 cut(s)	81
Bsp143I	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
BspACI	CCGC	9 cut(s)	55, 135, 149, 173, 176, 188, 262, 309, 476
BspANI	GGCC	4 cut(s)	194, 202, 586, 596
BspCNI	CTCAG	1 cut(s)	352
BspEI	TCCGGA	1 cut(s)	81
BspFNI	CGCG	2 cut(s)	173, 264
BspLI	GGNNCC	5 cut(s)	80, 86, 253, 519, 606
BspPI	GGATC	4 cut(s)	361, 371, 512, 525
BspT107I	GGYRCC	2 cut(s)	251, 604
BspTNI	GGTCTC	1 cut(s)	229
BsrDI	GCAATG	1 cut(s)	304
BsrFI	RCCGGY	1 cut(s)	293
BsrI	ACTGG	4 cut(s)	24, 400, 427, 635
BssAI	RCCGGY	1 cut(s)	293
BssMI	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
BssNI	GRCGYC	1 cut(s)	252
Bst2UI	CCWGG	1 cut(s)	735
Bst4CI	ACNGT	4 cut(s)	101, 269, 604, 746
Bst6I	CTCTTC	2 cut(s)	788, 792
BstACI	GRCGYC	1 cut(s)	252
BstC8I	GCNNGC	4 cut(s)	160, 295, 584, 598
BstDEI	CTNAG	1 cut(s)	360
BstF5I	GGATG	1 cut(s)	235
BstFNI	CGCG	2 cut(s)	173, 264
BstH2I	RGCGCY	2 cut(s)	96, 255
BstHHI	GCGC	3 cut(s)	95, 173, 254
BstKTI	GATC	8 cut(s)	327, 369, 379, 430, 520, 670, 769, 793
BstMAI	GTCTC	2 cut(s)	107, 229
BstMBI	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
BstMWI	GCNNNNNNNGC	8 cut(s)	71, 146, 155, 168, 208, 290, 299, 308
BstNI	CCWGG	1 cut(s)	735
BstNSI	RCATGY	2 cut(s)	413, 692
BstSCI	CCNGG	1 cut(s)	733
BstUI	CGCG	2 cut(s)	173, 264
BstX2I	RGATCY	4 cut(s)	427, 517, 667, 790
BstYI	RGATCY	4 cut(s)	427, 517, 667, 790
BsuI	GTATCC	3 cut(s)	175, 443, 666
BsuRI	GGCC	4 cut(s)	194, 202, 586, 596
BtsCI	GGATG	1 cut(s)	235
BtsIMutI	CAGTG	1 cut(s)	31
Cac8I	GCNNGC	4 cut(s)	160, 295, 584, 598
CfoI	GCGC	3 cut(s)	95, 173, 254
Cfr10I	RCCGGY	1 cut(s)	293
Cfr13I	GGNCC	2 cut(s)	21, 566
CseI	GACGC	1 cut(s)	200
Csp6I	GTAC	3 cut(s)	605, 738, 779
CviAII	CATG	4 cut(s)	410, 689, 710, 721
CviJI	RGCY	9 cut(s)	79, 87, 124, 194, 202, 364, 586, 596, 763
CviKI_1	RGCY	9 cut(s)	79, 87, 124, 194, 202, 364, 586, 596, 763
CviQI	GTAC	3 cut(s)	605, 738, 779
DdeI	CTNAG	1 cut(s)	360
DinI	GGCGCC	1 cut(s)	253
DpnI	GATC	8 cut(s)	326, 368, 378, 429, 519, 669, 768, 792
DpnII	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
EaeI	YGGCCR	1 cut(s)	192
Eam1104I	CTCTTC	2 cut(s)	788, 792
EarI	CTCTTC	2 cut(s)	788, 792
EciI	GGCGGA	1 cut(s)	298
Eco31I	GGTCTC	1 cut(s)	229
Eco32I	GATATC	1 cut(s)	439
Eco47I	GGWCC	2 cut(s)	21, 566
Eco57I	CTGAAG	1 cut(s)	671
EcoO109I	RGGNCCY	1 cut(s)	566
EcoRII	CCWGG	1 cut(s)	733
EcoRV	GATATC	1 cut(s)	439
EcoT22I	ATGCAT	1 cut(s)	690
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
Esp3I	CGTCTC	1 cut(s)	107
FaeI	CATG	4 cut(s)	413, 692, 713, 724
FalI	AAGNNNNNCTT	2 cut(s)	767, 799
FaqI	GGGAC	1 cut(s)	498
FatI	CATG	4 cut(s)	409, 688, 709, 720
FbaI	TGATCA	2 cut(s)	324, 766
FblI	GTMKAC	1 cut(s)	288
Fnu4HI	GCNGC	1 cut(s)	174
FokI	GGATG	1 cut(s)	222
Fsp4HI	GCNGC	1 cut(s)	174
FspBI	CTAG	2 cut(s)	119, 563
GlaI	GCGC	3 cut(s)	94, 172, 253
GluI	GCNGC	1 cut(s)	174
GsuI	CTGGAG	1 cut(s)	444
HaeII	RGCGCY	2 cut(s)	96, 255
HaeIII	GGCC	4 cut(s)	194, 202, 586, 596
HapII	CCGG	2 cut(s)	82, 294
HgaI	GACGC	1 cut(s)	200
HhaI	GCGC	3 cut(s)	95, 173, 254
Hin1I	GRCGYC	1 cut(s)	252
Hin1II	CATG	4 cut(s)	413, 692, 713, 724
Hin6I	GCGC	3 cut(s)	93, 171, 252
HinP1I	GCGC	3 cut(s)	93, 171, 252
HincII	GTYRAC	1 cut(s)	277
HindII	GTYRAC	1 cut(s)	277
HindIII	AAGCTT	1 cut(s)	761
HinfI	GANTC	5 cut(s)	36, 241, 497, 559, 713
HpaI	GTTAAC	1 cut(s)	277
HpaII	CCGG	2 cut(s)	82, 294
HphI	GGTGA	3 cut(s)	38, 163, 560
Hpy166II	GTNNAC	3 cut(s)	277, 289, 385
Hpy188I	TCNGA	5 cut(s)	129, 229, 240, 651, 788
Hpy188III	TCNNGA	4 cut(s)	82, 374, 569, 794
Hpy8I	GTNNAC	3 cut(s)	277, 289, 385
Hpy99I	CGWCG	5 cut(s)	148, 167, 216, 269, 464
HpyAV	CCTTC	4 cut(s)	148, 224, 618, 629
HpyCH4III	ACNGT	4 cut(s)	101, 269, 604, 746
HpyCH4V	TGCA	4 cut(s)	74, 527, 600, 688
HpyF10VI	GCNNNNNNNGC	8 cut(s)	71, 146, 155, 168, 208, 290, 299, 308
HpyF3I	CTNAG	1 cut(s)	360
Hsp92I	GRCGYC	1 cut(s)	252
Hsp92II	CATG	4 cut(s)	413, 692, 713, 724
HspAI	GCGC	3 cut(s)	93, 171, 252
KasI	GGCGCC	1 cut(s)	251
Kpn2I	TCCGGA	1 cut(s)	81
KpnI	GGTACC	1 cut(s)	608
KroI	GCCGGC	1 cut(s)	293
KroNI	GCCGGC	1 cut(s)	295
Ksp22I	TGATCA	2 cut(s)	324, 766
KspAI	GTTAAC	1 cut(s)	277
Kzo9I	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
LmnI	GCTCC	6 cut(s)	4, 34, 84, 84, 145, 264
MaeI	CTAG	2 cut(s)	119, 563
MaeIII	GTNAC	1 cut(s)	355
MalI	GATC	8 cut(s)	326, 368, 378, 429, 519, 669, 768, 792
MboI	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
MboII	GAAGA	2 cut(s)	775, 809
MfeI	CAATTG	1 cut(s)	106
MflI	RGATCY	4 cut(s)	427, 517, 667, 790
MhlI	GDGCHC	3 cut(s)	9, 31, 142
MluCI	AATT	5 cut(s)	106, 455, 551, 577, 728
Mly113I	GGCGCC	1 cut(s)	252
MlyI	GAGTC	3 cut(s)	30, 235, 568
MmeI	TCCRAC	1 cut(s)	263
Mph1103I	ATGCAT	1 cut(s)	690
MroI	TCCGGA	1 cut(s)	81
MroNI	GCCGGC	1 cut(s)	293
MseI	TTAA	2 cut(s)	276, 773
MslI	CAYNNNNRTG	1 cut(s)	492
MspA1I	CMGCKG	1 cut(s)	188
MspI	CCGG	2 cut(s)	82, 294
MspR9I	CCNGG	1 cut(s)	735
MunI	CAATTG	1 cut(s)	106
Mva1269I	GAATGC	1 cut(s)	120
MvaI	CCWGG	1 cut(s)	735
MvnI	CGCG	2 cut(s)	173, 264
MwoI	GCNNNNNNNGC	8 cut(s)	71, 146, 155, 168, 208, 290, 299, 308
NaeI	GCCGGC	1 cut(s)	295
NarI	GGCGCC	1 cut(s)	252
NdeII	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
NgoMIV	GCCGGC	1 cut(s)	293
NlaIII	CATG	4 cut(s)	413, 692, 713, 724
NlaIV	GGNNCC	5 cut(s)	80, 86, 253, 519, 606
NmeAIII	GCCGAG	1 cut(s)	220
NsiI	ATGCAT	1 cut(s)	690
NspI	RCATGY	2 cut(s)	413, 692
PctI	GAATGC	1 cut(s)	120
PdiI	GCCGGC	1 cut(s)	295
PfeI	GAWTC	2 cut(s)	497, 713
PkrI	GCNGC	1 cut(s)	175
PleI	GAGTC	3 cut(s)	30, 235, 567
PluTI	GGCGCC	1 cut(s)	255
PpsI	GAGTC	3 cut(s)	30, 235, 567
PpuMI	RGGWCCY	1 cut(s)	566
Psp5II	RGGWCCY	1 cut(s)	566
Psp6I	CCWGG	1 cut(s)	733
PspGI	CCWGG	1 cut(s)	733
PspN4I	GGNNCC	5 cut(s)	80, 86, 253, 519, 606
PspPI	GGNCC	2 cut(s)	21, 566
PspPPI	RGGWCCY	1 cut(s)	566
PsuI	RGATCY	4 cut(s)	427, 517, 667, 790
RsaI	GTAC	3 cut(s)	606, 739, 780
RsaNI	GTAC	3 cut(s)	605, 738, 779
RseI	CAYNNNNRTG	1 cut(s)	492
SaqAI	TTAA	2 cut(s)	276, 773
SatI	GCNGC	1 cut(s)	174
Sau3AI	GATC	8 cut(s)	324, 366, 376, 427, 517, 667, 766, 790
Sau96I	GGNCC	2 cut(s)	21, 566
SchI	GAGTC	3 cut(s)	30, 235, 568
ScrFI	CCNGG	1 cut(s)	735
SduI	GDGCHC	3 cut(s)	9, 31, 142
SfoI	GGCGCC	1 cut(s)	253
SinI	GGWCC	2 cut(s)	21, 566
SmiMI	CAYNNNNRTG	1 cut(s)	492
Sse9I	AATT	5 cut(s)	106, 455, 551, 577, 728
SsiI	CCGC	9 cut(s)	55, 135, 149, 173, 176, 188, 262, 309, 476
SspDI	GGCGCC	1 cut(s)	251
SspMI	CTAG	2 cut(s)	119, 563
StyD4I	CCNGG	1 cut(s)	733
TaaI	ACNGT	4 cut(s)	101, 269, 604, 746
TaqI	TCGA	2 cut(s)	459, 716
TasI	AATT	5 cut(s)	106, 455, 551, 577, 728
TauI	GCSGC	1 cut(s)	176
TfiI	GAWTC	2 cut(s)	497, 713
Tru1I	TTAA	2 cut(s)	276, 773
Tru9I	TTAA	2 cut(s)	276, 773
TscAI	CASTG	1 cut(s)	31
TspDTI	ATGAA	2 cut(s)	696, 726
TspGWI	ACGGA	2 cut(s)	132, 180
TspRI	CASTG	1 cut(s)	31
VpaK11BI	GGWCC	2 cut(s)	21, 566
XapI	RAATTY	2 cut(s)	455, 551
XceI	RCATGY	2 cut(s)	413, 692
XmiI	GTMKAC	1 cut(s)	288
XspI	CTAG	2 cut(s)	119, 563
Zsp2I	ATGCAT	1 cut(s)	690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.