Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

26063353 .. 26068287

Gene Structure 4935 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1240800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGGCCCAGCAGCAGCAGCTAACCAAGTGCAGCGCCTGGTCCACCGCTCCCAACCTCAATCTTCCCCGCCAGAACACCCAGCGTTTCGCCTTTCCCAATGCCAGGCGCCAAAACCCAAATCAGAAGACCAAGAGTCTGAATTGGTGGATGCGAGGGTTTGGGAATCATGCGAGGGCGTTGGTGGCGAAGGCGAGTAATGGCGGGGCAGGGACCGAGGTGGCGGAGAATACGGTGGTCGACAGGCCGAAACTGCAGCCCTTTCAGGTGTCTCAGGGTCATCCGAGTGCGCTCGGTGCCACGGCGCGTGATGGCGGGGTTAACTTCGCCGTCTATTCCGGCAATGCGGTTTCCGTCACTCTCTGTTTGATCACTCTCTCCGATTTAGAGGGGGATAAAGTTACTGAGCTGATCCCTCTTGATCCTTTGACCAACAAGACCGGAAATGTGTGGCATGTACTTCTCAAGGGAGATTTCAAAGATATCCTTTACGGATACAAATTTGATGGGAAGTTTTCGCCTGAAGAGGGACACTACTATGACTCTTCTAGAATTGTATTGGATCCTTATGCAAAGGCAGTTATTCGTAGAGGAGAATTCCGCAAGCTAGGTCCTGATGGTAATTGCTGGCCTCAAATGGCTGGAACGGTACCTTCTTTTGATGATCAGTTTGACTGGGAAGGAGATTTACCACTAAAATATCCACAAAAAGATCTTATCATATATGAAATGCATGTGCGAGGGTTCACAAGGCATGAATCGAGCGTGACTGACTTTCCTGGTACATACCTTGGTTTGGTGGAGAAACTTGATCATTTAAAGGAGCTTGGGATCAATTGTTTAGAGTTAATGCCATGTCATGAGTTCAATGAGTTGGAGTACTTCAGCTACAATTCTGTCTCGGGTGACTACAAGGTAAATTTTTGGGGATACTCAACTATCAATTGCTTTTCACCAATGATAAGATATTCTTCTGCTGGCATACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTCATTAAAGAAGCACATAAACGTGGAATTGAGGTGATCATGGATGTTGTCTTCAATCACACAGCTGAAGGGAATGAAAATGGCCCCATGTTGTCTTTTAGAGGTGCTGATAATAGTGTCTATTACATGCTTGCGCCTAAGAAAATCATATGCTTCTCAAATTTATTGGTTTTCTCCTTTGTTGATCTTGTTCAATACTTTAAATGTTTACGGTCCTTGAATCTAGTTGAGGACAATTTTAATGCTTTTAACATGGTGGGTTTATTTGCTATTGCTATTTCTGTTGTTCCATGTAATTGGATGTTTGCTTTAATCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

447

Amino Acids

50.18

Weight (kDa)

6.56

Isoelectric Point (pI)

30.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	97 - 190	1.2e-14	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	253 - 364	1.2e-08	Alpha amylase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	646
AccB1I	GGYRCC	3 cut(s)	105, 293, 646
AccBSI	CCGCTC	1 cut(s)	47
AccI	GTMKAC	1 cut(s)	237
AccII	CGCG	1 cut(s)	304
AciI	CCGC	7 cut(s)	45, 67, 201, 221, 312, 344, 598
AclWI	GGATC	5 cut(s)	403, 413, 554, 567, 836
AcoI	YGGCCR	1 cut(s)	991
AcsI	RAATTY	4 cut(s)	497, 593, 916, 1180
AcuI	CTGAAG	3 cut(s)	540, 865, 1107
AcyI	GRCGYC	1 cut(s)	106
AfaI	GTAC	4 cut(s)	456, 648, 781, 878
AfiI	CCNNNNNNNGG	3 cut(s)	102, 524, 793
AgsI	TTSAA	5 cut(s)	475, 865, 1075, 1214, 1240
AhdI	GACNNNNNGTC	1 cut(s)	132
AjnI	CCWGG	3 cut(s)	35, 101, 775
AluBI	AGCT	6 cut(s)	19, 406, 604, 823, 885, 1085
AluI	AGCT	6 cut(s)	19, 406, 604, 823, 885, 1085
Alw26I	GTCTC	2 cut(s)	273, 901
AlwI	GGATC	5 cut(s)	403, 413, 554, 567, 836
AlwNI	CAGNNNCTG	1 cut(s)	36
Ama87I	CYCGRG	1 cut(s)	898
AoxI	GGCC	5 cut(s)	3, 242, 626, 991, 1102
ApeKI	GCWGC	5 cut(s)	10, 13, 16, 30, 253
ApoI	RAATTY	4 cut(s)	497, 593, 916, 1180
Asp718I	GGTACC	1 cut(s)	646
AspLEI	GCGC	5 cut(s)	35, 108, 289, 304, 1156
AspS9I	GGNCC	6 cut(s)	4, 39, 210, 608, 1103, 1233
AsuHPI	GGTGA	3 cut(s)	914, 942, 1066
AvaI	CYCGRG	1 cut(s)	898
AvaII	GGWCC	4 cut(s)	39, 210, 608, 1233
BamHI	GGATCC	1 cut(s)	559
BanI	GGYRCC	3 cut(s)	105, 293, 646
BarI	GAAGNNNNNNTAC	2 cut(s)	669, 701
BbsI	GAAGAC	2 cut(s)	131, 1063
BbvI	GCAGC	5 cut(s)	22, 25, 28, 42, 265
BccI	CCATC	3 cut(s)	302, 497, 608
BceAI	ACGGC	3 cut(s)	311, 315, 978
BciT130I	CCWGG	3 cut(s)	37, 103, 777
BciVI	GTATCC	2 cut(s)	485, 920
BclI	TGATCA	4 cut(s)	366, 661, 808, 1056
BcoDI	GTCTC	2 cut(s)	273, 901
BfaI	CTAG	3 cut(s)	546, 605, 1244
BfmI	CTRYAG	1 cut(s)	251
BfoI	RGCGCY	2 cut(s)	36, 109
BfuI	GTATCC	2 cut(s)	485, 920
BglII	AGATCT	1 cut(s)	709
BisI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
BlsI	GCNGC	5 cut(s)	12, 15, 18, 32, 255
BmcAI	AGTACT	1 cut(s)	878
Bme1390I	CCNGG	3 cut(s)	37, 103, 777
Bme18I	GGWCC	4 cut(s)	39, 210, 608, 1233
BmeRI	GACNNNNNGTC	1 cut(s)	132
BmeT110I	CYCGRG	1 cut(s)	898
BmgT120I	GGNCC	6 cut(s)	4, 39, 210, 608, 1103, 1233
BmiI	GGNNCC	6 cut(s)	107, 211, 295, 561, 648, 1105
BmrFI	CCNGG	3 cut(s)	37, 103, 777
BmrI	ACTGGG	1 cut(s)	682
BmsI	GCATC	2 cut(s)	138, 988
BmuI	ACTGGG	1 cut(s)	682
BpiI	GAAGAC	2 cut(s)	131, 1063
BpuEI	CTTGAG	1 cut(s)	446
BsaAI	YACGTR	1 cut(s)	983
BsaHI	GRCGYC	1 cut(s)	106
BsaJI	CCNNGG	3 cut(s)	213, 297, 787
BsaWI	WCCGGW	1 cut(s)	437
Bsc4I	CCNNNNNNNGG	3 cut(s)	102, 524, 793
Bse1I	ACTGG	1 cut(s)	677
Bse3DI	GCAATG	1 cut(s)	346
BseBI	CCWGG	3 cut(s)	37, 103, 777
BseDI	CCNNGG	3 cut(s)	213, 297, 787
BseGI	GGATG	4 cut(s)	153, 277, 1069, 1326
BseLI	CCNNNNNNNGG	3 cut(s)	102, 524, 793
BseMI	GCAATG	1 cut(s)	346
BseMII	CTCAG	2 cut(s)	284, 393
BseNI	ACTGG	1 cut(s)	677
BseRI	GAGGAG	1 cut(s)	603
BseXI	GCAGC	5 cut(s)	22, 25, 28, 42, 265
BseYI	CCCAGC	2 cut(s)	6, 78
BsgI	GTGCAG	1 cut(s)	49
Bsh1236I	CGCG	1 cut(s)	304
BshFI	GGCC	5 cut(s)	5, 244, 628, 993, 1104
BshNI	GGYRCC	3 cut(s)	105, 293, 646
BsiHKCI	CYCGRG	1 cut(s)	898
BsiSI	CCGG	2 cut(s)	336, 438
BslFI	GGGAC	2 cut(s)	223, 540
BslI	CCNNNNNNNGG	3 cut(s)	102, 524, 793
BsmAI	GTCTC	2 cut(s)	273, 901
BsmFI	GGGAC	2 cut(s)	223, 540
BsnI	GGCC	5 cut(s)	5, 244, 628, 993, 1104
BsoBI	CYCGRG	1 cut(s)	898
BspACI	CCGC	7 cut(s)	45, 67, 201, 221, 312, 344, 598
BspANI	GGCC	5 cut(s)	5, 244, 628, 993, 1104
BspCNI	CTCAG	2 cut(s)	283, 394
BspFNI	CGCG	1 cut(s)	304
BspHI	TCATGA	1 cut(s)	856
BspLI	GGNNCC	6 cut(s)	107, 211, 295, 561, 648, 1105
BspMAI	CTGCAG	1 cut(s)	255
BspPI	GGATC	5 cut(s)	403, 413, 554, 567, 836
BspT107I	GGYRCC	3 cut(s)	105, 293, 646
BsrBI	CCGCTC	1 cut(s)	47
BsrDI	GCAATG	1 cut(s)	346
BsrI	ACTGG	1 cut(s)	677
BssECI	CCNNGG	3 cut(s)	213, 297, 787
BssNI	GRCGYC	1 cut(s)	106
BssT1I	CCWWGG	1 cut(s)	787
Bst2UI	CCWGG	3 cut(s)	37, 103, 777
Bst4CI	ACNGT	3 cut(s)	232, 646, 1233
Bst6I	CTCTTC	2 cut(s)	516, 547
BstACI	GRCGYC	1 cut(s)	106
BstBAI	YACGTR	1 cut(s)	983
BstC8I	GCNNGC	4 cut(s)	602, 626, 976, 1152
BstDEI	CTNAG	3 cut(s)	270, 402, 1158
BstDSI	CCRYGG	1 cut(s)	297
BstF5I	GGATG	4 cut(s)	153, 277, 1069, 1326
BstFNI	CGCG	1 cut(s)	304
BstH2I	RGCGCY	2 cut(s)	36, 109
BstHHI	GCGC	5 cut(s)	35, 108, 289, 304, 1156
BstMAI	GTCTC	2 cut(s)	273, 901
BstMWI	GCNNNNNNNGC	4 cut(s)	16, 182, 250, 293
BstNI	CCWGG	3 cut(s)	37, 103, 777
BstNSI	RCATGY	3 cut(s)	455, 734, 1150
BstSCI	CCNGG	3 cut(s)	35, 101, 775
BstSFI	CTRYAG	1 cut(s)	251
BstSNI	TACGTA	1 cut(s)	983
BstUI	CGCG	1 cut(s)	304
BstV1I	GCAGC	5 cut(s)	22, 25, 28, 42, 265
BstV2I	GAAGAC	2 cut(s)	131, 1063
BstX2I	RGATCY	2 cut(s)	559, 709
BstXI	CCANNNNNNTGG	1 cut(s)	1317
BstYI	RGATCY	2 cut(s)	559, 709
BsuI	GTATCC	2 cut(s)	485, 920
BsuRI	GGCC	5 cut(s)	5, 244, 628, 993, 1104
BtgI	CCRYGG	1 cut(s)	297
BtsCI	GGATG	4 cut(s)	153, 277, 1069, 1326
Cac8I	GCNNGC	4 cut(s)	602, 626, 976, 1152
CaiI	CAGNNNCTG	1 cut(s)	36
CciI	TCATGA	1 cut(s)	856
CfoI	GCGC	5 cut(s)	35, 108, 289, 304, 1156
Cfr13I	GGNCC	6 cut(s)	4, 39, 210, 608, 1103, 1233
Csp6I	GTAC	4 cut(s)	455, 647, 780, 877
CviQI	GTAC	4 cut(s)	455, 647, 780, 877
DdeI	CTNAG	3 cut(s)	270, 402, 1158
DinI	GGCGCC	1 cut(s)	107
DraI	TTTAAA	2 cut(s)	816, 1222
DriI	GACNNNNNGTC	1 cut(s)	132
EaeI	YGGCCR	1 cut(s)	991
Eam1104I	CTCTTC	2 cut(s)	516, 547
Eam1105I	GACNNNNNGTC	1 cut(s)	132
EarI	CTCTTC	2 cut(s)	516, 547
EciI	GGCGGA	1 cut(s)	236
Eco105I	TACGTA	1 cut(s)	983
Eco130I	CCWWGG	1 cut(s)	787
Eco32I	GATATC	1 cut(s)	481
Eco47I	GGWCC	4 cut(s)	39, 210, 608, 1233
Eco57I	CTGAAG	3 cut(s)	540, 865, 1107
Eco88I	CYCGRG	1 cut(s)	898
EcoO109I	RGGNCCY	1 cut(s)	608
EcoRI	GAATTC	1 cut(s)	593
EcoRII	CCWGG	3 cut(s)	35, 101, 775
EcoRV	GATATC	1 cut(s)	481
EcoT14I	CCWWGG	1 cut(s)	787
EcoT22I	ATGCAT	1 cut(s)	732
EgeI	GGCGCC	1 cut(s)	107
EheI	GGCGCC	1 cut(s)	107
ErhI	CCWWGG	1 cut(s)	787
FalI	AAGNNNNNCTT	4 cut(s)	468, 500, 952, 984
FaqI	GGGAC	2 cut(s)	223, 540
FauI	CCCGC	3 cut(s)	74, 194, 305
FauNDI	CATATG	1 cut(s)	1169
FbaI	TGATCA	4 cut(s)	366, 661, 808, 1056
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
FokI	GGATG	4 cut(s)	160, 264, 1076, 1333
Fsp4HI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
FspBI	CTAG	3 cut(s)	546, 605, 1244
GlaI	GCGC	5 cut(s)	34, 107, 288, 303, 1155
GluI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
GsaI	CCCAGC	2 cut(s)	10, 82
HaeII	RGCGCY	2 cut(s)	36, 109
HaeIII	GGCC	5 cut(s)	5, 244, 628, 993, 1104
HapII	CCGG	2 cut(s)	336, 438
HhaI	GCGC	5 cut(s)	35, 108, 289, 304, 1156
Hin1I	GRCGYC	1 cut(s)	106
Hin6I	GCGC	5 cut(s)	33, 106, 287, 302, 1154
HinP1I	GCGC	5 cut(s)	33, 106, 287, 302, 1154
HincII	GTYRAC	2 cut(s)	238, 319
HindII	GTYRAC	2 cut(s)	238, 319
HinfI	GANTC	5 cut(s)	133, 163, 539, 755, 1240
HpaI	GTTAAC	1 cut(s)	319
HpaII	CCGG	2 cut(s)	336, 438
HphI	GGTGA	3 cut(s)	914, 942, 1066
Hpy166II	GTNNAC	5 cut(s)	42, 238, 319, 744, 1229
Hpy188I	TCNGA	4 cut(s)	123, 138, 282, 379
Hpy188III	TCNNGA	4 cut(s)	416, 546, 611, 857
Hpy8I	GTNNAC	5 cut(s)	42, 238, 319, 744, 1229
HpyAV	CCTTC	4 cut(s)	181, 660, 671, 1082
HpyCH4III	ACNGT	3 cut(s)	232, 646, 1233
HpyCH4IV	ACGT	2 cut(s)	982, 1042
HpyCH4V	TGCA	5 cut(s)	30, 253, 569, 730, 1001
HpyF10VI	GCNNNNNNNGC	4 cut(s)	16, 182, 250, 293
HpyF3I	CTNAG	3 cut(s)	270, 402, 1158
HpySE526I	ACGT	2 cut(s)	982, 1042
Hsp92I	GRCGYC	1 cut(s)	106
HspAI	GCGC	5 cut(s)	33, 106, 287, 302, 1154
KasI	GGCGCC	1 cut(s)	105
KpnI	GGTACC	1 cut(s)	650
Ksp22I	TGATCA	4 cut(s)	366, 661, 808, 1056
KspAI	GTTAAC	1 cut(s)	319
LmnI	GCTCC	2 cut(s)	52, 820
Lsp1109I	GCAGC	5 cut(s)	22, 25, 28, 42, 265
LweI	GCATC	2 cut(s)	138, 988
MaeI	CTAG	3 cut(s)	546, 605, 1244
MaeII	ACGT	2 cut(s)	982, 1042
MaeIII	GTNAC	4 cut(s)	352, 397, 763, 902
MbiI	CCGCTC	1 cut(s)	47
MboII	GAAGA	6 cut(s)	53, 136, 533, 534, 960, 1063
MfeI	CAATTG	2 cut(s)	832, 940
MflI	RGATCY	2 cut(s)	559, 709
Mly113I	GGCGCC	1 cut(s)	106
MlyI	GAGTC	2 cut(s)	142, 533
MmeI	TCCRAC	1 cut(s)	852
Mph1103I	ATGCAT	1 cut(s)	732
MseI	TTAA	8 cut(s)	318, 815, 845, 1026, 1221, 1260, 1269, 1331
MslI	CAYNNNNRTG	3 cut(s)	282, 534, 1041
MspA1I	CMGCKG	1 cut(s)	1085
MspI	CCGG	2 cut(s)	336, 438
MspR9I	CCNGG	3 cut(s)	37, 103, 777
MunI	CAATTG	2 cut(s)	832, 940
MvaI	CCWGG	3 cut(s)	37, 103, 777
MvnI	CGCG	1 cut(s)	304
MwoI	GCNNNNNNNGC	4 cut(s)	16, 182, 250, 293
NarI	GGCGCC	1 cut(s)	106
NdeI	CATATG	1 cut(s)	1169
NlaIV	GGNNCC	6 cut(s)	107, 211, 295, 561, 648, 1105
NmuCI	GTSAC	3 cut(s)	352, 763, 902
NsiI	ATGCAT	1 cut(s)	732
NspI	RCATGY	3 cut(s)	455, 734, 1150
PagI	TCATGA	1 cut(s)	856
PfeI	GAWTC	3 cut(s)	163, 755, 1240
PkrI	GCNGC	5 cut(s)	12, 15, 18, 32, 255
PleI	GAGTC	2 cut(s)	141, 533
PluTI	GGCGCC	1 cut(s)	109
PpsI	GAGTC	2 cut(s)	141, 533
Ppu21I	YACGTR	1 cut(s)	983
PpuMI	RGGWCCY	1 cut(s)	608
Psp5II	RGGWCCY	1 cut(s)	608
Psp6I	CCWGG	3 cut(s)	35, 101, 775
PspFI	CCCAGC	2 cut(s)	6, 78
PspGI	CCWGG	3 cut(s)	35, 101, 775
PspN4I	GGNNCC	6 cut(s)	107, 211, 295, 561, 648, 1105
PspPI	GGNCC	6 cut(s)	4, 39, 210, 608, 1103, 1233
PspPPI	RGGWCCY	1 cut(s)	608
PstI	CTGCAG	1 cut(s)	255
PstNI	CAGNNNCTG	1 cut(s)	36
PsuI	RGATCY	2 cut(s)	559, 709
PvuII	CAGCTG	1 cut(s)	1085
RsaI	GTAC	4 cut(s)	456, 648, 781, 878
RsaNI	GTAC	4 cut(s)	455, 647, 780, 877
RseI	CAYNNNNRTG	3 cut(s)	282, 534, 1041
SalI	GTCGAC	1 cut(s)	236
SaqAI	TTAA	8 cut(s)	318, 815, 845, 1026, 1221, 1260, 1269, 1331
SatI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
Sau96I	GGNCC	6 cut(s)	4, 39, 210, 608, 1103, 1233
ScaI	AGTACT	1 cut(s)	878
SchI	GAGTC	2 cut(s)	142, 533
ScrFI	CCNGG	3 cut(s)	37, 103, 777
SfaNI	GCATC	2 cut(s)	138, 988
SfcI	CTRYAG	1 cut(s)	251
SfoI	GGCGCC	1 cut(s)	107
SinI	GGWCC	4 cut(s)	39, 210, 608, 1233
SmiMI	CAYNNNNRTG	3 cut(s)	282, 534, 1041
SmlI	CTYRAG	1 cut(s)	461
SmoI	CTYRAG	1 cut(s)	461
SnaBI	TACGTA	1 cut(s)	983
SsiI	CCGC	7 cut(s)	45, 67, 201, 221, 312, 344, 598
SspDI	GGCGCC	1 cut(s)	105
SspMI	CTAG	3 cut(s)	546, 605, 1244
StyD4I	CCNGG	3 cut(s)	35, 101, 775
StyI	CCWWGG	1 cut(s)	787
TaaI	ACNGT	3 cut(s)	232, 646, 1233
TaiI	ACGT	2 cut(s)	985, 1045
TaqI	TCGA	2 cut(s)	237, 758
TaqII	GACCGA	1 cut(s)	227
TatI	WGTACW	2 cut(s)	454, 876
TfiI	GAWTC	3 cut(s)	163, 755, 1240
Tru1I	TTAA	8 cut(s)	318, 815, 845, 1026, 1221, 1260, 1269, 1331
Tru9I	TTAA	8 cut(s)	318, 815, 845, 1026, 1221, 1260, 1269, 1331
TseFI	GTSAC	3 cut(s)	352, 763, 902
TseI	GCWGC	5 cut(s)	10, 13, 16, 30, 253
Tsp45I	GTSAC	3 cut(s)	352, 763, 902
TspDTI	ATGAA	4 cut(s)	738, 768, 1023, 1110
TspGWI	ACGGA	2 cut(s)	340, 504
VpaK11BI	GGWCC	4 cut(s)	39, 210, 608, 1233
XapI	RAATTY	4 cut(s)	497, 593, 916, 1180
XbaI	TCTAGA	1 cut(s)	545
XceI	RCATGY	3 cut(s)	455, 734, 1150
XmiI	GTMKAC	1 cut(s)	237
XspI	CTAG	3 cut(s)	546, 605, 1244
ZrmI	AGTACT	1 cut(s)	878
Zsp2I	ATGCAT	1 cut(s)	732

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.