Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

5740122 .. 5749333

Gene Structure 9212 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g06440.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGGCCTGCATGGTACCTTCTTTTGATGATAAGTTCAACTGGGAAGGAGATTTACCTCTGAATTATCCACAAAAGGATCTTATCATATACGAAATGCATGTGCGAGGTTTTACAAGGCATGAATCGAGCATGACTGAATTGCCTGGTACGTACCTTGGTTTGGTGGAGAAGCTTGATCACTTGAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCATGTCATGAGTTTAATGAACTGGAGTACTTTGGCTACAATTCTGTCTTGGGTGACTACAAGGTAAATTATTGGGGGTATTCGACTGTCAATTTCTTTTCACCAATGATAAGATATTCATCTGCTGGCACACGTAACTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTGTTAAAGAAGCACATAGACGTGGAATTGAGGTGATCATGGATGTTGTTTTCAATCACACAGCTGAAGGGAATGAAAATGGTCCGATTTTGTCTTTTAGAGGTGCTGACAACAGTGTCTATTACATGCTTGCACCTAAGGGAGAGTTTTATAATTATTCAGGGTGTGGTAACACATTCAATTGCAACCATCCTGTTGTGCGACAGTTCATAGTAGATTGCTTGAGATATTGGGTAACAGAGATGCACGTGGATGGGTTCCGCTTCGATCTTGCTTCTATTATGACCAGGGGTACCAGTCTCTGGGATTCAACTAATGTATACGGGAGTGTGATAGAAGGTGACCTGTTGACAACTGGAACCCCTCTCGCTAGTCCTCCATTGATTGACATGATTAGTAATGACCCAATACTTCATGGAGTGAAGCTGATAGCTGAAGCTTGGGATGCAGGAGGCTTGTACCAACTTGGCATGTTTCCTCAGTGGGGTAATTGGTCAGAATGGAATGGGAAGTATCGTGACACAGTGCGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTATGGCCAAATGCCTTTGTGGGAGCCCTAATTTGTACCAGGAAGGGGGAGGAAGACCATGGAACAGCATCAATTTTATTTGTGCACACGATGGCTTTACTTTAGCTGATTTAGTAACCTATAACAACAAACATAACCTGGCAAATGGAGAAGACAACAATGACGGAGAGAATCATAATAATAGCTGGAACTGTGGACAGGAGGGAGAATTCGCAAGCATTTCAGTGAGAAAACTGAGGAAACGTCAAATGCGGAATTTTTTCGTCTGCCTCATGGTTTCCCAAGGTGTTCCGATGATATATATGGGTGATGAATATGGTCATACAAAAGGGGGTAACAACAACACATATTGCCATGATAATTATATTAATTACTTCCGGTGGGATAAGAAGGAAGAGTCTTCATCGGATTTTTTCAGATTTTGCTGTCTTATGACCAAATTCCGCCAGGAATGTGAGTCACTTGGTTTGAATTACTTCCCAACAGCAGAGAGGCTGCAGTGGCATGGTCTTGCTCCTGGGGTGCCAGACTGGTCTGAAACTAGCCGCTTTGTGGCCTTTACTCTGGTTGACTCGGTGAAGAGAGAGATCTACATTGCCTTCAACGCCAGTCATTTACCAGTGAATGTATCACTGCCAGAGAGGCATGGATACAGATGGGAACCCTTGGTAGACACCAGCAAGCCTTCACCCTTTGACTTCCTGTCTAGTGACGTCCCACAAAGAGATATTGCAGTTAAACAGTATGCTCACTTTCTTGACGCCAATTTATACCCGATGCTAAGTTATTCTTCAATCATCCTATTGCTCGCCCCAGTCGAAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

594

Amino Acids

67.61

Weight (kDa)

5.31

Isoelectric Point (pI)

39.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	86 - 180	1.4e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	244 - 354	1.5e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	685 - 786	2.1e-46	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	540
AasI	GACNNNNNNGTC	1 cut(s)	503
AatII	GACGTC	1 cut(s)	1668
Acc65I	GGTACC	2 cut(s)	13, 680
AccB1I	GGYRCC	3 cut(s)	13, 680, 1474
AccB7I	CCANNNNNTGG	1 cut(s)	690
AccI	GTMKAC	2 cut(s)	708, 1623
AciI	CCGC	5 cut(s)	649, 916, 1204, 1396, 1498
AclWI	GGATC	2 cut(s)	84, 203
AcoI	YGGCCR	2 cut(s)	358, 957
AcsI	RAATTY	3 cut(s)	1160, 1207, 1391
AcuI	CTGAAG	2 cut(s)	474, 843
AcvI	CACGTG	1 cut(s)	637
AcyI	GRCGYC	2 cut(s)	1665, 1713
AfaI	GTAC	8 cut(s)	15, 148, 152, 245, 682, 848, 934, 989
AfiI	CCNNNNNNNGG	5 cut(s)	160, 690, 872, 997, 1504
AflIII	ACRYGT	1 cut(s)	347
AgsI	TTSAA	8 cut(s)	37, 184, 442, 568, 699, 1423, 1555, 1746
AhdI	GACNNNNNGTC	1 cut(s)	1654
AjiI	CACGTC	1 cut(s)	410
AjnI	CCWGG	6 cut(s)	142, 674, 990, 1089, 1398, 1468
AluBI	AGCT	7 cut(s)	172, 452, 814, 821, 827, 1058, 1137
AluI	AGCT	7 cut(s)	172, 452, 814, 821, 827, 1058, 1137
Alw21I	GWGCWC	1 cut(s)	1039
Alw26I	GTCTC	1 cut(s)	692
Alw44I	GTGCAC	1 cut(s)	1035
AlwI	GGATC	2 cut(s)	84, 203
AlwNI	CAGNNNCTG	1 cut(s)	690
AoxI	GGCC	4 cut(s)	3, 358, 957, 1506
ApaLI	GTGCAC	1 cut(s)	1035
ApeKI	GCWGC	1 cut(s)	1447
ApoI	RAATTY	3 cut(s)	1160, 1207, 1391
AseI	ATTAAT	1 cut(s)	1320
Asp700I	GAANNNNTTC	2 cut(s)	1211, 1427
Asp718I	GGTACC	2 cut(s)	13, 680
AspS9I	GGNCC	1 cut(s)	470
AsuHPI	GGTGA	7 cut(s)	281, 309, 433, 740, 1271, 1540, 1632
AvaII	GGWCC	1 cut(s)	470
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	1 cut(s)	1039
BalI	TGGCCA	1 cut(s)	959
BanI	GGYRCC	3 cut(s)	13, 680, 1474
BanII	GRGCYC	1 cut(s)	980
BarI	GAAGNNNNNNTAC	2 cut(s)	36, 68
BbrPI	CACGTG	1 cut(s)	637
BbsI	GAAGAC	3 cut(s)	1012, 1110, 1344
Bbv12I	GWGCWC	1 cut(s)	1039
BbvI	GCAGC	1 cut(s)	1434
BccI	CCATC	5 cut(s)	585, 635, 932, 1037, 1602
BceAI	ACGGC	1 cut(s)	345
BciT130I	CCWGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
BciVI	GTATCC	1 cut(s)	1595
BclI	TGATCA	2 cut(s)	175, 423
BcoDI	GTCTC	1 cut(s)	692
BfaI	CTAG	3 cut(s)	759, 1494, 1659
BfmI	CTRYAG	1 cut(s)	1448
BfuI	GTATCC	1 cut(s)	1595
BglI	GCCNNNNNGGC	1 cut(s)	1594
BglII	AGATCT	1 cut(s)	1539
BisI	GCNGC	3 cut(s)	917, 1448, 1498
BlsI	GCNGC	3 cut(s)	918, 1449, 1499
BmcAI	AGTACT	1 cut(s)	245
Bme1390I	CCNGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
Bme18I	GGWCC	1 cut(s)	470
BmeRI	GACNNNNNGTC	1 cut(s)	1654
BmgBI	CACGTC	1 cut(s)	410
BmgT120I	GGNCC	1 cut(s)	470
BmiI	GGNNCC	7 cut(s)	15, 647, 682, 748, 977, 1476, 1614
BmrFI	CCNGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
BmrI	ACTGGG	2 cut(s)	49, 1760
BmsI	GCATC	6 cut(s)	355, 621, 823, 1029, 1719, 1765
BmuI	ACTGGG	2 cut(s)	49, 1760
BpiI	GAAGAC	3 cut(s)	1012, 1110, 1344
BpmI	CTGGAG	1 cut(s)	260
BpuEI	CTTGAG	1 cut(s)	631
BsaAI	YACGTR	3 cut(s)	150, 350, 637
BsaBI	GATNNNNATC	1 cut(s)	334
BsaHI	GRCGYC	2 cut(s)	1665, 1713
BsaJI	CCNNGG	6 cut(s)	154, 675, 1010, 1234, 1469, 1617
BsaWI	WCCGGW	1 cut(s)	1329
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 690, 872, 997, 1504
Bse1I	ACTGG	8 cut(s)	44, 243, 684, 748, 1487, 1560, 1571, 1766
Bse21I	CCTNAGG	1 cut(s)	525
Bse3DI	GCAATG	1 cut(s)	1545
Bse8I	GATNNNNATC	1 cut(s)	334
BseBI	CCWGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
BseDI	CCNNGG	6 cut(s)	154, 675, 1010, 1234, 1469, 1617
BseGI	GGATG	5 cut(s)	436, 577, 646, 838, 1749
BseJI	GATNNNNATC	1 cut(s)	334
BseLI	CCNNNNNNNGG	5 cut(s)	160, 690, 872, 997, 1504
BseMI	GCAATG	1 cut(s)	1545
BseMII	CTCAG	2 cut(s)	881, 1178
BseNI	ACTGG	8 cut(s)	44, 243, 684, 748, 1487, 1560, 1571, 1766
BseSI	GKGCMC	1 cut(s)	1039
BseXI	GCAGC	1 cut(s)	1434
BshFI	GGCC	4 cut(s)	5, 360, 959, 1508
BshNI	GGYRCC	3 cut(s)	13, 680, 1474
BsiHKAI	GWGCWC	1 cut(s)	1039
BsiSI	CCGG	1 cut(s)	1330
BslFI	GGGAC	1 cut(s)	1652
BslI	CCNNNNNNNGG	5 cut(s)	160, 690, 872, 997, 1504
BsmAI	GTCTC	1 cut(s)	692
BsmFI	GGGAC	1 cut(s)	1652
BsnI	GGCC	4 cut(s)	5, 360, 959, 1508
Bsp1286I	GDGCHC	2 cut(s)	980, 1039
Bsp143I	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
Bsp19I	CCATGG	1 cut(s)	1010
BspACI	CCGC	5 cut(s)	649, 916, 1204, 1396, 1498
BspANI	GGCC	4 cut(s)	5, 360, 959, 1508
BspCNI	CTCAG	2 cut(s)	880, 1179
BspHI	TCATGA	1 cut(s)	223
BspLI	GGNNCC	7 cut(s)	15, 647, 682, 748, 977, 1476, 1614
BspMAI	CTGCAG	1 cut(s)	1452
BspPI	GGATC	2 cut(s)	84, 203
BspT107I	GGYRCC	3 cut(s)	13, 680, 1474
BsrDI	GCAATG	1 cut(s)	1545
BsrI	ACTGG	8 cut(s)	44, 243, 684, 748, 1487, 1560, 1571, 1766
BssECI	CCNNGG	6 cut(s)	154, 675, 1010, 1234, 1469, 1617
BssMI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
BssNAI	GTATAC	1 cut(s)	709
BssNI	GRCGYC	2 cut(s)	1665, 1713
BssT1I	CCWWGG	4 cut(s)	154, 1010, 1234, 1617
Bst1107I	GTATAC	1 cut(s)	709
Bst2UI	CCWGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
Bst4CI	ACNGT	8 cut(s)	203, 304, 356, 503, 594, 913, 1145, 1695
Bst6I	CTCTTC	2 cut(s)	1341, 1526
BstACI	GRCGYC	2 cut(s)	1665, 1713
BstBAI	YACGTR	3 cut(s)	150, 350, 637
BstC8I	GCNNGC	6 cut(s)	7, 343, 519, 1168, 1634, 1761
BstDEI	CTNAG	5 cut(s)	525, 867, 1187, 1733, 1779
BstDSI	CCRYGG	1 cut(s)	1010
BstEII	GGTNACC	1 cut(s)	728
BstF5I	GGATG	5 cut(s)	436, 577, 646, 838, 1749
BstKTI	GATC	6 cut(s)	79, 178, 198, 426, 658, 1542
BstMAI	GTCTC	1 cut(s)	692
BstMBI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
BstMWI	GCNNNNNNNGC	4 cut(s)	833, 1453, 1556, 1594
BstNI	CCWGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
BstNSI	RCATGY	3 cut(s)	101, 517, 862
BstPI	GGTNACC	1 cut(s)	728
BstSCI	CCNGG	6 cut(s)	142, 674, 990, 1089, 1398, 1468
BstSFI	CTRYAG	1 cut(s)	1448
BstSLI	GKGCMC	1 cut(s)	1039
BstSNI	TACGTA	1 cut(s)	150
BstV1I	GCAGC	1 cut(s)	1434
BstV2I	GAAGAC	3 cut(s)	1012, 1110, 1344
BstX2I	RGATCY	2 cut(s)	76, 1539
BstYI	RGATCY	2 cut(s)	76, 1539
BstZ17I	GTATAC	1 cut(s)	709
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	1 cut(s)	1595
BsuRI	GGCC	4 cut(s)	5, 360, 959, 1508
BtgI	CCRYGG	1 cut(s)	1010
BtrI	CACGTC	1 cut(s)	410
BtsCI	GGATG	5 cut(s)	436, 577, 646, 838, 1749
BtsI	GCAGTG	2 cut(s)	1457, 1583
BtsIMutI	CAGTG	7 cut(s)	508, 875, 918, 1182, 1457, 1578, 1583
Cac8I	GCNNGC	6 cut(s)	7, 343, 519, 1168, 1634, 1761
CaiI	CAGNNNCTG	1 cut(s)	690
CciI	TCATGA	1 cut(s)	223
Cfr13I	GGNCC	1 cut(s)	470
CseI	GACGC	1 cut(s)	1721
Csp6I	GTAC	8 cut(s)	14, 147, 151, 244, 681, 847, 933, 988
CviQI	GTAC	8 cut(s)	14, 147, 151, 244, 681, 847, 933, 988
DdeI	CTNAG	5 cut(s)	525, 867, 1187, 1733, 1779
DpnI	GATC	6 cut(s)	78, 177, 197, 425, 657, 1541
DpnII	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
DrdI	GACNNNNNNGTC	1 cut(s)	503
DriI	GACNNNNNGTC	1 cut(s)	1654
DseDI	GACNNNNNNGTC	1 cut(s)	503
EaeI	YGGCCR	2 cut(s)	358, 957
Eam1104I	CTCTTC	2 cut(s)	1341, 1526
Eam1105I	GACNNNNNGTC	1 cut(s)	1654
EarI	CTCTTC	2 cut(s)	1341, 1526
EciI	GGCGGA	1 cut(s)	1385
Eco105I	TACGTA	1 cut(s)	150
Eco130I	CCWWGG	4 cut(s)	154, 1010, 1234, 1617
Eco24I	GRGCYC	1 cut(s)	980
Eco47I	GGWCC	1 cut(s)	470
Eco57I	CTGAAG	2 cut(s)	474, 843
Eco72I	CACGTG	1 cut(s)	637
Eco81I	CCTNAGG	1 cut(s)	525
Eco91I	GGTNACC	1 cut(s)	728
EcoO65I	GGTNACC	1 cut(s)	728
EcoRI	GAATTC	1 cut(s)	1160
EcoRII	CCWGG	6 cut(s)	142, 674, 990, 1089, 1398, 1468
EcoT14I	CCWWGG	4 cut(s)	154, 1010, 1234, 1617
EcoT22I	ATGCAT	1 cut(s)	99
EcoT38I	GRGCYC	1 cut(s)	980
ErhI	CCWWGG	4 cut(s)	154, 1010, 1234, 1617
FalI	AAGNNNNNCTT	2 cut(s)	1726, 1758
FaqI	GGGAC	1 cut(s)	1652
FbaI	TGATCA	2 cut(s)	175, 423
FblI	GTMKAC	2 cut(s)	708, 1623
Fnu4HI	GCNGC	3 cut(s)	917, 1448, 1498
FokI	GGATG	5 cut(s)	443, 564, 653, 845, 1736
FriOI	GRGCYC	1 cut(s)	980
Fsp4HI	GCNGC	3 cut(s)	917, 1448, 1498
FspBI	CTAG	3 cut(s)	759, 1494, 1659
GluI	GCNGC	3 cut(s)	917, 1448, 1498
GsuI	CTGGAG	1 cut(s)	260
HaeIII	GGCC	4 cut(s)	5, 360, 959, 1508
HapII	CCGG	1 cut(s)	1330
HgaI	GACGC	1 cut(s)	1721
Hin1I	GRCGYC	2 cut(s)	1665, 1713
HincII	GTYRAC	2 cut(s)	738, 1522
HindII	GTYRAC	2 cut(s)	738, 1522
HindIII	AAGCTT	2 cut(s)	170, 825
HinfI	GANTC	6 cut(s)	122, 695, 1123, 1349, 1409, 1523
HpaII	CCGG	1 cut(s)	1330
HphI	GGTGA	7 cut(s)	281, 309, 433, 740, 1271, 1540, 1632
Hpy166II	GTNNAC	6 cut(s)	709, 738, 1037, 1148, 1522, 1624
Hpy188I	TCNGA	7 cut(s)	60, 474, 886, 1245, 1360, 1370, 1489
Hpy188III	TCNNGA	3 cut(s)	224, 905, 1709
Hpy8I	GTNNAC	6 cut(s)	709, 738, 1037, 1148, 1522, 1624
HpyAV	CCTTC	9 cut(s)	27, 38, 178, 449, 719, 989, 1336, 1561, 1647
HpyCH4III	ACNGT	8 cut(s)	203, 304, 356, 503, 594, 913, 1145, 1695
HpyCH4IV	ACGT	6 cut(s)	149, 349, 409, 636, 1195, 1665
HpyF10VI	GCNNNNNNNGC	4 cut(s)	833, 1453, 1556, 1594
HpyF3I	CTNAG	5 cut(s)	525, 867, 1187, 1733, 1779
HpySE526I	ACGT	6 cut(s)	149, 349, 409, 636, 1195, 1665
Hsp92I	GRCGYC	2 cut(s)	1665, 1713
KpnI	GGTACC	2 cut(s)	17, 684
Ksp22I	TGATCA	2 cut(s)	175, 423
Kzo9I	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
LmnI	GCTCC	2 cut(s)	975, 1471
Lsp1109I	GCAGC	1 cut(s)	1434
LweI	GCATC	6 cut(s)	355, 621, 823, 1029, 1719, 1765
MaeI	CTAG	3 cut(s)	759, 1494, 1659
MaeII	ACGT	6 cut(s)	149, 349, 409, 636, 1195, 1665
MalI	GATC	6 cut(s)	78, 177, 197, 425, 657, 1541
MboI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
MboII	GAAGA	6 cut(s)	1017, 1115, 1344, 1358, 1543, 1734
MfeI	CAATTG	1 cut(s)	568
MflI	RGATCY	2 cut(s)	76, 1539
MhlI	GDGCHC	2 cut(s)	980, 1039
MlsI	TGGCCA	1 cut(s)	959
MluNI	TGGCCA	1 cut(s)	959
MlyI	GAGTC	3 cut(s)	1358, 1418, 1517
Mox20I	TGGCCA	1 cut(s)	959
Mph1103I	ATGCAT	1 cut(s)	99
MroXI	GAANNNNTTC	2 cut(s)	1211, 1427
MscI	TGGCCA	1 cut(s)	959
MseI	TTAA	5 cut(s)	212, 231, 393, 1320, 1689
MslI	CAYNNNNRTG	3 cut(s)	408, 639, 1175
Msp20I	TGGCCA	1 cut(s)	959
MspA1I	CMGCKG	1 cut(s)	452
MspI	CCGG	1 cut(s)	1330
MspR9I	CCNGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
MunI	CAATTG	1 cut(s)	568
MvaI	CCWGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
MwoI	GCNNNNNNNGC	4 cut(s)	833, 1453, 1556, 1594
NcoI	CCATGG	1 cut(s)	1010
NdeII	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
NlaIV	GGNNCC	7 cut(s)	15, 647, 682, 748, 977, 1476, 1614
NmuCI	GTSAC	5 cut(s)	269, 728, 905, 1410, 1661
NsiI	ATGCAT	1 cut(s)	99
NspI	RCATGY	3 cut(s)	101, 517, 862
PagI	TCATGA	1 cut(s)	223
PcsI	WCGNNNNNNNCGW	1 cut(s)	1767
PdmI	GAANNNNTTC	2 cut(s)	1211, 1427
PfeI	GAWTC	3 cut(s)	122, 695, 1123
PflMI	CCANNNNNTGG	1 cut(s)	690
PkrI	GCNGC	3 cut(s)	918, 1449, 1499
PleI	GAGTC	3 cut(s)	1357, 1417, 1517
PmaCI	CACGTG	1 cut(s)	637
PmlI	CACGTG	1 cut(s)	637
PpsI	GAGTC	3 cut(s)	1357, 1417, 1517
Ppu21I	YACGTR	3 cut(s)	150, 350, 637
PshBI	ATTAAT	1 cut(s)	1320
PsiI	TTATAA	1 cut(s)	540
Psp6I	CCWGG	6 cut(s)	142, 674, 990, 1089, 1398, 1468
PspCI	CACGTG	1 cut(s)	637
PspEI	GGTNACC	1 cut(s)	728
PspGI	CCWGG	6 cut(s)	142, 674, 990, 1089, 1398, 1468
PspN4I	GGNNCC	7 cut(s)	15, 647, 682, 748, 977, 1476, 1614
PspPI	GGNCC	1 cut(s)	470
PstI	CTGCAG	1 cut(s)	1452
PstNI	CAGNNNCTG	1 cut(s)	690
PsuI	RGATCY	2 cut(s)	76, 1539
PvuII	CAGCTG	1 cut(s)	452
RsaI	GTAC	8 cut(s)	15, 148, 152, 245, 682, 848, 934, 989
RsaNI	GTAC	8 cut(s)	14, 147, 151, 244, 681, 847, 933, 988
RseI	CAYNNNNRTG	3 cut(s)	408, 639, 1175
SaqAI	TTAA	5 cut(s)	212, 231, 393, 1320, 1689
SatI	GCNGC	3 cut(s)	917, 1448, 1498
Sau3AI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1539
Sau96I	GGNCC	1 cut(s)	470
ScaI	AGTACT	1 cut(s)	245
SchI	GAGTC	3 cut(s)	1358, 1418, 1517
ScrFI	CCNGG	6 cut(s)	144, 676, 992, 1091, 1400, 1470
SduI	GDGCHC	2 cut(s)	980, 1039
SfaNI	GCATC	6 cut(s)	355, 621, 823, 1029, 1719, 1765
SfcI	CTRYAG	1 cut(s)	1448
SinI	GGWCC	1 cut(s)	470
SmiMI	CAYNNNNRTG	3 cut(s)	408, 639, 1175
SmlI	CTYRAG	1 cut(s)	610
SmoI	CTYRAG	1 cut(s)	610
SnaBI	TACGTA	1 cut(s)	150
SsiI	CCGC	5 cut(s)	649, 916, 1204, 1396, 1498
SspMI	CTAG	3 cut(s)	759, 1494, 1659
StyD4I	CCNGG	6 cut(s)	142, 674, 990, 1089, 1398, 1468
StyI	CCWWGG	4 cut(s)	154, 1010, 1234, 1617
TaaI	ACNGT	8 cut(s)	203, 304, 356, 503, 594, 913, 1145, 1695
TaiI	ACGT	6 cut(s)	152, 352, 412, 639, 1198, 1668
TaqI	TCGA	4 cut(s)	125, 299, 654, 1770
TatI	WGTACW	1 cut(s)	243
TauI	GCSGC	2 cut(s)	919, 1500
TfiI	GAWTC	3 cut(s)	122, 695, 1123
Tru1I	TTAA	5 cut(s)	212, 231, 393, 1320, 1689
Tru9I	TTAA	5 cut(s)	212, 231, 393, 1320, 1689
TscAI	CASTG	7 cut(s)	508, 875, 918, 1182, 1457, 1578, 1590
TseFI	GTSAC	5 cut(s)	269, 728, 905, 1410, 1661
TseI	GCWGC	1 cut(s)	1447
Tsp45I	GTSAC	5 cut(s)	269, 728, 905, 1410, 1661
TspDTI	ATGAA	9 cut(s)	135, 249, 324, 390, 477, 586, 791, 1278, 1344
TspGWI	ACGGA	1 cut(s)	1131
TspRI	CASTG	7 cut(s)	508, 875, 918, 1182, 1457, 1578, 1590
Van91I	CCANNNNNTGG	1 cut(s)	690
VneI	GTGCAC	1 cut(s)	1035
VpaK11BI	GGWCC	1 cut(s)	470
VspI	ATTAAT	1 cut(s)	1320
XapI	RAATTY	3 cut(s)	1160, 1207, 1391
XceI	RCATGY	3 cut(s)	101, 517, 862
XmiI	GTMKAC	2 cut(s)	708, 1623
XmnI	GAANNNNTTC	2 cut(s)	1211, 1427
XspI	CTAG	3 cut(s)	759, 1494, 1659
ZraI	GACGTC	1 cut(s)	1666
ZrmI	AGTACT	1 cut(s)	245
Zsp2I	ATGCAT	1 cut(s)	99

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.