Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

26052307 .. 26053519

Gene Structure 1213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1240700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 693 bp

ATGGCCCAGCAGCAGCAGCTAACCAAGTGCAGCGCCTGGTCCACCGCTCCCAACCTCAATCTTCCCCGCCAGAACACCCAGCGTTTCGCCTTTCCCAATGCCAGGCGCCAAAACCCAAATCAGAAGACCAAGAGTCTGAATTGGTGGATGCGAGGGTTTGGGAATCATGCGAGGGCGTTGGTGGCGAAGGCGAGTAATGGCGGGGCAGGGACCGAGGTGGCGGAGAATACGGTGGTCGACAGGCCGAAACTGCAGCCCTTTCAGGTGTCTCAGGGTCATCCGAGTGCGCTCGGTGCCACGGCGCGTGATGGCGGGGTTAACTTCGCCGTCTATTCCGGCAATGCGGTTTCCGTCACTCTCTGTTTGATCACTCTCTCCGATTTAGAGGGGGATAAAGTTACTGAGCTGATCCCTCTTGATCCTTTGACCAACAAGACCGGAAATGTGTGGCATGTACTTCTCAAGGGAGATTTCAAAGATATCCTTTACGGATACAAATTTGATGGGAAGTTTTCGCCTGAAGAGGGACACTACTATGACTCTTCTAGAATTGTATTGGATCCTTATGCAAAGGCAGTTATTCGTAGAGGAGAATTCCGCAAGCTAGGTCCTGATGGTAATTGCTGGCCTCAAATGGCTGGAACGGTACCTTCTTTTGATGATCAGGTACTTTATCCACAAAATCTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

231

Amino Acids

25.44

Weight (kDa)

9.11

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	97 - 190	3.2e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	646
AccB1I	GGYRCC	3 cut(s)	105, 293, 646
AccBSI	CCGCTC	1 cut(s)	47
AccI	GTMKAC	1 cut(s)	237
AccII	CGCG	1 cut(s)	304
AciI	CCGC	7 cut(s)	45, 67, 201, 221, 312, 344, 598
AclWI	GGATC	4 cut(s)	403, 413, 554, 567
AcsI	RAATTY	2 cut(s)	497, 593
AcuI	CTGAAG	1 cut(s)	540
AcyI	GRCGYC	1 cut(s)	106
AfaI	GTAC	3 cut(s)	456, 648, 669
AfiI	CCNNNNNNNGG	2 cut(s)	102, 524
AgsI	TTSAA	1 cut(s)	475
AhdI	GACNNNNNGTC	1 cut(s)	132
AjnI	CCWGG	2 cut(s)	35, 101
AluBI	AGCT	3 cut(s)	19, 406, 604
AluI	AGCT	3 cut(s)	19, 406, 604
Alw26I	GTCTC	1 cut(s)	273
AlwI	GGATC	4 cut(s)	403, 413, 554, 567
AlwNI	CAGNNNCTG	1 cut(s)	36
AoxI	GGCC	3 cut(s)	3, 242, 626
ApeKI	GCWGC	5 cut(s)	10, 13, 16, 30, 253
ApoI	RAATTY	2 cut(s)	497, 593
Asp718I	GGTACC	1 cut(s)	646
AspLEI	GCGC	4 cut(s)	35, 108, 289, 304
AspS9I	GGNCC	4 cut(s)	4, 39, 210, 608
AvaII	GGWCC	3 cut(s)	39, 210, 608
BamHI	GGATCC	1 cut(s)	559
BanI	GGYRCC	3 cut(s)	105, 293, 646
BbsI	GAAGAC	1 cut(s)	131
BbvI	GCAGC	5 cut(s)	22, 25, 28, 42, 265
BccI	CCATC	3 cut(s)	302, 497, 608
BceAI	ACGGC	2 cut(s)	311, 315
BciT130I	CCWGG	2 cut(s)	37, 103
BciVI	GTATCC	1 cut(s)	485
BclI	TGATCA	2 cut(s)	366, 661
BcoDI	GTCTC	1 cut(s)	273
BfaI	CTAG	2 cut(s)	546, 605
BfmI	CTRYAG	1 cut(s)	251
BfoI	RGCGCY	2 cut(s)	36, 109
BfuI	GTATCC	1 cut(s)	485
BisI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
BlsI	GCNGC	5 cut(s)	12, 15, 18, 32, 255
Bme1390I	CCNGG	2 cut(s)	37, 103
Bme18I	GGWCC	3 cut(s)	39, 210, 608
BmeRI	GACNNNNNGTC	1 cut(s)	132
BmgT120I	GGNCC	4 cut(s)	4, 39, 210, 608
BmiI	GGNNCC	5 cut(s)	107, 211, 295, 561, 648
BmrFI	CCNGG	2 cut(s)	37, 103
BmsI	GCATC	1 cut(s)	138
BpiI	GAAGAC	1 cut(s)	131
BpuEI	CTTGAG	1 cut(s)	446
BsaHI	GRCGYC	1 cut(s)	106
BsaJI	CCNNGG	2 cut(s)	213, 297
BsaWI	WCCGGW	1 cut(s)	437
Bsc4I	CCNNNNNNNGG	2 cut(s)	102, 524
Bse3DI	GCAATG	1 cut(s)	346
BseBI	CCWGG	2 cut(s)	37, 103
BseDI	CCNNGG	2 cut(s)	213, 297
BseGI	GGATG	2 cut(s)	153, 277
BseLI	CCNNNNNNNGG	2 cut(s)	102, 524
BseMI	GCAATG	1 cut(s)	346
BseMII	CTCAG	2 cut(s)	284, 393
BseRI	GAGGAG	1 cut(s)	603
BseXI	GCAGC	5 cut(s)	22, 25, 28, 42, 265
BseYI	CCCAGC	2 cut(s)	6, 78
BsgI	GTGCAG	1 cut(s)	49
Bsh1236I	CGCG	1 cut(s)	304
BshFI	GGCC	3 cut(s)	5, 244, 628
BshNI	GGYRCC	3 cut(s)	105, 293, 646
BsiSI	CCGG	2 cut(s)	336, 438
BslFI	GGGAC	2 cut(s)	223, 540
BslI	CCNNNNNNNGG	2 cut(s)	102, 524
BsmAI	GTCTC	1 cut(s)	273
BsmFI	GGGAC	2 cut(s)	223, 540
BsnI	GGCC	3 cut(s)	5, 244, 628
Bsp143I	GATC	5 cut(s)	366, 408, 418, 559, 661
BspACI	CCGC	7 cut(s)	45, 67, 201, 221, 312, 344, 598
BspANI	GGCC	3 cut(s)	5, 244, 628
BspCNI	CTCAG	2 cut(s)	283, 394
BspFNI	CGCG	1 cut(s)	304
BspLI	GGNNCC	5 cut(s)	107, 211, 295, 561, 648
BspMAI	CTGCAG	1 cut(s)	255
BspPI	GGATC	4 cut(s)	403, 413, 554, 567
BspT107I	GGYRCC	3 cut(s)	105, 293, 646
BsrBI	CCGCTC	1 cut(s)	47
BsrDI	GCAATG	1 cut(s)	346
BssECI	CCNNGG	2 cut(s)	213, 297
BssMI	GATC	5 cut(s)	366, 408, 418, 559, 661
BssNI	GRCGYC	1 cut(s)	106
Bst2UI	CCWGG	2 cut(s)	37, 103
Bst4CI	ACNGT	2 cut(s)	232, 646
Bst6I	CTCTTC	2 cut(s)	516, 547
BstACI	GRCGYC	1 cut(s)	106
BstC8I	GCNNGC	2 cut(s)	602, 626
BstDEI	CTNAG	2 cut(s)	270, 402
BstDSI	CCRYGG	1 cut(s)	297
BstF5I	GGATG	2 cut(s)	153, 277
BstFNI	CGCG	1 cut(s)	304
BstH2I	RGCGCY	2 cut(s)	36, 109
BstHHI	GCGC	4 cut(s)	35, 108, 289, 304
BstKTI	GATC	5 cut(s)	369, 411, 421, 562, 664
BstMAI	GTCTC	1 cut(s)	273
BstMBI	GATC	5 cut(s)	366, 408, 418, 559, 661
BstMWI	GCNNNNNNNGC	4 cut(s)	16, 182, 250, 293
BstNI	CCWGG	2 cut(s)	37, 103
BstNSI	RCATGY	1 cut(s)	455
BstSCI	CCNGG	2 cut(s)	35, 101
BstSFI	CTRYAG	1 cut(s)	251
BstUI	CGCG	1 cut(s)	304
BstV1I	GCAGC	5 cut(s)	22, 25, 28, 42, 265
BstV2I	GAAGAC	1 cut(s)	131
BstX2I	RGATCY	1 cut(s)	559
BstYI	RGATCY	1 cut(s)	559
BsuI	GTATCC	1 cut(s)	485
BsuRI	GGCC	3 cut(s)	5, 244, 628
BtgI	CCRYGG	1 cut(s)	297
BtsCI	GGATG	2 cut(s)	153, 277
Cac8I	GCNNGC	2 cut(s)	602, 626
CaiI	CAGNNNCTG	1 cut(s)	36
CfoI	GCGC	4 cut(s)	35, 108, 289, 304
Cfr13I	GGNCC	4 cut(s)	4, 39, 210, 608
Csp6I	GTAC	3 cut(s)	455, 647, 668
CviAII	CATG	2 cut(s)	167, 452
CviJI	RGCY	8 cut(s)	5, 19, 244, 256, 406, 604, 628, 638
CviKI_1	RGCY	8 cut(s)	5, 19, 244, 256, 406, 604, 628, 638
CviQI	GTAC	3 cut(s)	455, 647, 668
DdeI	CTNAG	2 cut(s)	270, 402
DinI	GGCGCC	1 cut(s)	107
DpnI	GATC	5 cut(s)	368, 410, 420, 561, 663
DpnII	GATC	5 cut(s)	366, 408, 418, 559, 661
DriI	GACNNNNNGTC	1 cut(s)	132
Eam1104I	CTCTTC	2 cut(s)	516, 547
Eam1105I	GACNNNNNGTC	1 cut(s)	132
EarI	CTCTTC	2 cut(s)	516, 547
EciI	GGCGGA	1 cut(s)	236
Eco32I	GATATC	1 cut(s)	481
Eco47I	GGWCC	3 cut(s)	39, 210, 608
Eco57I	CTGAAG	1 cut(s)	540
EcoO109I	RGGNCCY	1 cut(s)	608
EcoRI	GAATTC	1 cut(s)	593
EcoRII	CCWGG	2 cut(s)	35, 101
EcoRV	GATATC	1 cut(s)	481
EgeI	GGCGCC	1 cut(s)	107
EheI	GGCGCC	1 cut(s)	107
FaeI	CATG	2 cut(s)	170, 455
FaiI	YATR	4 cut(s)	168, 453, 537, 567
FalI	AAGNNNNNCTT	2 cut(s)	468, 500
FaqI	GGGAC	2 cut(s)	223, 540
FatI	CATG	2 cut(s)	166, 451
FauI	CCCGC	3 cut(s)	74, 194, 305
FbaI	TGATCA	2 cut(s)	366, 661
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
FokI	GGATG	2 cut(s)	160, 264
Fsp4HI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
FspBI	CTAG	2 cut(s)	546, 605
GlaI	GCGC	4 cut(s)	34, 107, 288, 303
GluI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
GsaI	CCCAGC	2 cut(s)	10, 82
HaeII	RGCGCY	2 cut(s)	36, 109
HaeIII	GGCC	3 cut(s)	5, 244, 628
HapII	CCGG	2 cut(s)	336, 438
HhaI	GCGC	4 cut(s)	35, 108, 289, 304
Hin1I	GRCGYC	1 cut(s)	106
Hin1II	CATG	2 cut(s)	170, 455
Hin6I	GCGC	4 cut(s)	33, 106, 287, 302
HinP1I	GCGC	4 cut(s)	33, 106, 287, 302
HincII	GTYRAC	2 cut(s)	238, 319
HindII	GTYRAC	2 cut(s)	238, 319
HinfI	GANTC	3 cut(s)	133, 163, 539
HpaI	GTTAAC	1 cut(s)	319
HpaII	CCGG	2 cut(s)	336, 438
Hpy166II	GTNNAC	3 cut(s)	42, 238, 319
Hpy188I	TCNGA	4 cut(s)	123, 138, 282, 379
Hpy188III	TCNNGA	3 cut(s)	416, 546, 611
Hpy8I	GTNNAC	3 cut(s)	42, 238, 319
HpyAV	CCTTC	2 cut(s)	181, 660
HpyCH4III	ACNGT	2 cut(s)	232, 646
HpyCH4V	TGCA	3 cut(s)	30, 253, 569
HpyF10VI	GCNNNNNNNGC	4 cut(s)	16, 182, 250, 293
HpyF3I	CTNAG	2 cut(s)	270, 402
Hsp92I	GRCGYC	1 cut(s)	106
Hsp92II	CATG	2 cut(s)	170, 455
HspAI	GCGC	4 cut(s)	33, 106, 287, 302
KasI	GGCGCC	1 cut(s)	105
KpnI	GGTACC	1 cut(s)	650
Ksp22I	TGATCA	2 cut(s)	366, 661
KspAI	GTTAAC	1 cut(s)	319
Kzo9I	GATC	5 cut(s)	366, 408, 418, 559, 661
LmnI	GCTCC	1 cut(s)	52
Lsp1109I	GCAGC	5 cut(s)	22, 25, 28, 42, 265
LweI	GCATC	1 cut(s)	138
MaeI	CTAG	2 cut(s)	546, 605
MaeIII	GTNAC	2 cut(s)	352, 397
MalI	GATC	5 cut(s)	368, 410, 420, 561, 663
MbiI	CCGCTC	1 cut(s)	47
MboI	GATC	5 cut(s)	366, 408, 418, 559, 661
MboII	GAAGA	4 cut(s)	53, 136, 533, 534
MflI	RGATCY	1 cut(s)	559
MluCI	AATT	5 cut(s)	139, 497, 549, 593, 619
Mly113I	GGCGCC	1 cut(s)	106
MlyI	GAGTC	2 cut(s)	142, 533
MnlI	CCTC	9 cut(s)	65, 146, 165, 208, 379, 423, 517, 581, 639
MseI	TTAA	1 cut(s)	318
MslI	CAYNNNNRTG	2 cut(s)	282, 534
MspI	CCGG	2 cut(s)	336, 438
MspR9I	CCNGG	2 cut(s)	37, 103
MvaI	CCWGG	2 cut(s)	37, 103
MvnI	CGCG	1 cut(s)	304
MwoI	GCNNNNNNNGC	4 cut(s)	16, 182, 250, 293
NarI	GGCGCC	1 cut(s)	106
NdeII	GATC	5 cut(s)	366, 408, 418, 559, 661
NlaIII	CATG	2 cut(s)	170, 455
NlaIV	GGNNCC	5 cut(s)	107, 211, 295, 561, 648
NmuCI	GTSAC	1 cut(s)	352
NspI	RCATGY	1 cut(s)	455
PfeI	GAWTC	1 cut(s)	163
PkrI	GCNGC	5 cut(s)	12, 15, 18, 32, 255
PleI	GAGTC	2 cut(s)	141, 533
PluTI	GGCGCC	1 cut(s)	109
PpsI	GAGTC	2 cut(s)	141, 533
PpuMI	RGGWCCY	1 cut(s)	608
Psp5II	RGGWCCY	1 cut(s)	608
Psp6I	CCWGG	2 cut(s)	35, 101
PspFI	CCCAGC	2 cut(s)	6, 78
PspGI	CCWGG	2 cut(s)	35, 101
PspN4I	GGNNCC	5 cut(s)	107, 211, 295, 561, 648
PspPI	GGNCC	4 cut(s)	4, 39, 210, 608
PspPPI	RGGWCCY	1 cut(s)	608
PstI	CTGCAG	1 cut(s)	255
PstNI	CAGNNNCTG	1 cut(s)	36
PsuI	RGATCY	1 cut(s)	559
RsaI	GTAC	3 cut(s)	456, 648, 669
RsaNI	GTAC	3 cut(s)	455, 647, 668
RseI	CAYNNNNRTG	2 cut(s)	282, 534
SalI	GTCGAC	1 cut(s)	236
SaqAI	TTAA	1 cut(s)	318
SatI	GCNGC	5 cut(s)	11, 14, 17, 31, 254
Sau3AI	GATC	5 cut(s)	366, 408, 418, 559, 661
Sau96I	GGNCC	4 cut(s)	4, 39, 210, 608
SchI	GAGTC	2 cut(s)	142, 533
ScrFI	CCNGG	2 cut(s)	37, 103
SetI	ASST	9 cut(s)	21, 57, 219, 267, 408, 606, 610, 652, 669
SfaNI	GCATC	1 cut(s)	138
SfcI	CTRYAG	1 cut(s)	251
SfoI	GGCGCC	1 cut(s)	107
SinI	GGWCC	3 cut(s)	39, 210, 608
SmiMI	CAYNNNNRTG	2 cut(s)	282, 534
SmlI	CTYRAG	1 cut(s)	461
SmoI	CTYRAG	1 cut(s)	461
Sse9I	AATT	5 cut(s)	139, 497, 549, 593, 619
SsiI	CCGC	7 cut(s)	45, 67, 201, 221, 312, 344, 598
SspDI	GGCGCC	1 cut(s)	105
SspMI	CTAG	2 cut(s)	546, 605
StyD4I	CCNGG	2 cut(s)	35, 101
TaaI	ACNGT	2 cut(s)	232, 646
TaqI	TCGA	1 cut(s)	237
TaqII	GACCGA	1 cut(s)	227
TasI	AATT	5 cut(s)	139, 497, 549, 593, 619
TatI	WGTACW	1 cut(s)	454
TfiI	GAWTC	1 cut(s)	163
Tru1I	TTAA	1 cut(s)	318
Tru9I	TTAA	1 cut(s)	318
TseFI	GTSAC	1 cut(s)	352
TseI	GCWGC	5 cut(s)	10, 13, 16, 30, 253
Tsp45I	GTSAC	1 cut(s)	352
TspGWI	ACGGA	2 cut(s)	340, 504
VpaK11BI	GGWCC	3 cut(s)	39, 210, 608
XapI	RAATTY	2 cut(s)	497, 593
XbaI	TCTAGA	1 cut(s)	545
XceI	RCATGY	1 cut(s)	455
XmiI	GTMKAC	1 cut(s)	237
XspI	CTAG	2 cut(s)	546, 605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.