Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

23832136 .. 23852037

Gene Structure 19902 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1236000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2406 bp

ATGGCGCAACAGCAGCTAACCAAGTGCAGCACCTGGCCCACCGCTCCCAATCTCCGTCTTCCCCGCCAAAACCCCCAGCGCTTTGCCTTTCCTAATGCCGGGCACCGGAATCCCAATCAGAAGAGTTTGAATTGGCGGATGCGAGGGTTTGGCGGTGATGCTCCGGCGGTGGCGGTGAAGGCGAGTAATGGCGGGGCAGGGACTGAGCTGGCGGAGACTGCGGTGGTGGTCGACAGGCCGAAACTGCGTCGTTTTCAAGTGTCCGATGGGTATCCGAGTCCGTTCGGTGCCACGGCGCGTGATGGCGGGGTTAACTTCGCCGTCTATTCCGGCAGTGCGGTTTCCGTCACTCTCTGTTTGATCACTCTCTCCGATTTAGAGGAGGATAAAGTTACTGAGCAGATTCCGCTTGATGCTTCGACCAACAAGTCTGGAAATGTGTGGCATGTACTTCTCAAGGGAGATTTCAAAGATACCCTTTATGGTTACAAATTTGATGGGAAGTTTTCGCCCGAAGAGGGACACTACTATGACTCTTCTAGAATTGTATTGGATCCTTATGCAAAGGCAGTTATCCGCAGAGGAGAATTCCGCAAGCTAGGTCCTGATGGTAACTGCTGGCCTCAAATGGCTGGAACGGTACCTTCTTTTAATGATCAGTTTGACTGGGAAGGAGATTTACCACTGAAGTATCCACAAAAAGACCTTATCATATATGAAATGCATGTGCGTGGGTTCACAAGGCATGAATCAAGTGCGACTGAGTTTCCTGGTACATACCTTGGTTTGGTGGACAATCTTGATCATTTAAAGGAGCTCGGGATCAACTGTTTAGAGTTAATGCCATGTCATGAGTTCAACGAGTTGGAGTACTTCAGCTACAATTCTGTCTTGGGTGACTATAAGGTAAATTTTTGGGGATACTCGACTGTCAATTACTTTTCACCAATGATAAGATATTCATCTGCTGGCATACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTATTAGAGAAGCACATAAACGTGGAATTGAGGTGATCATGGATGTCGTCTTCAATCACACAGCTGAAGGGAATGAAAATGGTCCCATTTTGTCTTTTAGAGGTGCTGATAATAGTGTCTATTACATGCTTGCGCCTAAGGGAGAGTTCTATAATTATTCGGGATGCGGTAACACATTCAATTGCAACCATCCTGTTGTGCGCCAGTTTATTGTAGACTGCTTGAGATATTGGGTAACAGAGATGCATGTGGATGGGTTCCGCTTTGATCTTGCTTCTATTATGACTAGGGGTAGCAGTCTCTGGGATGCAATTAATGTATATGGGAGTGCAATAGAAGGTGACTTGTTGACAACGGGAACCCCTCTTGCTACCCCACCATTGGTTGACATGATAAGTAATGACCCAATCCTTCATGGAGTGAAGCTTGTAGCTGAAGCATGGGATGCAGGAGGCCTGTACCAAGTTGGCATGTTTCCTCACTGGGGTAATTGGTCAGAATGGAATGGGAAGTATCGTGACACAGTGCGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTTTGGCTGAATGCCTTTGTGGGAGCCCCAACTTATATCAGGAAGGGGGGAGGCCATGGAGTAGTGTCAATTTTGTTTGTGCACACGACGGTTTTACTTTAACTGATTTAGTAACCTATAACAACAAACATAACCTGGCAAATGGAGAAGACAACAATGACGGAGAGAGTCATAATAATAGCTGGAACTGTGGACAGGAGGGAGAGTTTGCAAGCATTTCAGTGAAGAAATTGAGGAAGCGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTCTCCCAAGGTGTCCCAATGCTGTGTATGGGTGATGAATACGGCCATACAAAAGGAGGAAACAACAACACATATTGCCATGACAATTATATTAATTACTTTCGATGGGATAAGAAAGAAGAGTCCTCATCAGACTTTTTCAGATTTTGCTGCCTCATGACCAAATTCCGCCAGGAATGCGAGTCACTTGGGTTAAATGACTTCCCAACAGCAGAGAGGCTGCAGTGGCATGGTCATGCTCCAGGGGTGCCAGACTGGTCTGACACAAGCCGCTTTGTTGCCTTTACTCTGGTGGACTCTGTGAAGAGAGAGCTGTATATTGCTTTCAATGCCAGCCACTTAGCCGTGACCATTTCTCTGCCAGAGAGGCCTGGATACAGATGGGAGCCCTTGGTCGACACCAGCAAGTGCACACCATTTGACTTCCTTTCTAGTGACGTCCCAGAAAGAGATACCGCAGTTAAGCAGTATGCTCACTTTCTCGACGCCAATTTATATCCCATGCTAAGTTATTCTTCAATCATCCTATTACTCTCTCCCGTTGAAGATTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

802

Amino Acids

90.17

Weight (kDa)

5.54

Isoelectric Point (pI)

36.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	94 - 188	2.3e-14	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	252 - 362	1.8e-08	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	692 - 793	7.4e-47	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	427
AatII	GACGTC	1 cut(s)	2292
Acc65I	GGTACC	1 cut(s)	640
AccB1I	GGYRCC	4 cut(s)	102, 287, 640, 2098
AccBSI	CCGCTC	1 cut(s)	44
AccI	GTMKAC	3 cut(s)	231, 1230, 2247
AccII	CGCG	1 cut(s)	298
AclWI	GGATC	3 cut(s)	548, 561, 830
AcoI	YGGCCR	2 cut(s)	985, 1894
AcsI	RAATTY	5 cut(s)	491, 587, 910, 1831, 2015
AcuI	CTGAAG	4 cut(s)	707, 859, 1101, 1470
AcyI	GRCGYC	2 cut(s)	2289, 2337
AfaI	GTAC	6 cut(s)	450, 642, 775, 872, 1475, 1561
AfeI	AGCGCT	1 cut(s)	80
AfiI	CCNNNNNNNGG	6 cut(s)	98, 105, 518, 787, 1396, 1499
AgsI	TTSAA	9 cut(s)	130, 257, 469, 859, 1069, 1195, 2179, 2370, 2396
AjnI	CCWGG	6 cut(s)	32, 769, 1713, 2022, 2092, 2221
Alw21I	GWGCWC	3 cut(s)	819, 1663, 2264
Alw26I	GTCTC	3 cut(s)	209, 1319, 1858
Alw44I	GTGCAC	2 cut(s)	1659, 2260
AlwI	GGATC	3 cut(s)	548, 561, 830
AlwNI	CAGNNNCTG	3 cut(s)	33, 203, 1317
Ama87I	CYCGRG	1 cut(s)	818
Aor51HI	AGCGCT	1 cut(s)	80
AoxI	GGCC	8 cut(s)	35, 236, 620, 985, 1468, 1631, 1894, 2219
ApaLI	GTGCAC	2 cut(s)	1659, 2260
ApeKI	GCWGC	4 cut(s)	13, 27, 2001, 2071
ApoI	RAATTY	5 cut(s)	491, 587, 910, 1831, 2015
AseI	ATTAAT	2 cut(s)	1329, 1944
Asp718I	GGTACC	1 cut(s)	640
AspLEI	GCGC	5 cut(s)	7, 81, 298, 1150, 1218
AspS9I	GGNCC	3 cut(s)	36, 602, 1097
AsuC2I	CCSGG	1 cut(s)	100
AsuHPI	GGTGA	7 cut(s)	167, 187, 908, 936, 1060, 1367, 1895
AvaI	CYCGRG	1 cut(s)	818
AvaII	GGWCC	2 cut(s)	602, 1097
AxyI	CCTNAGG	1 cut(s)	1152
BaeGI	GKGCMC	3 cut(s)	105, 1663, 2264
BaeI	ACNNNNGTAYC	4 cut(s)	674, 707, 2295, 2328
BamHI	GGATCC	1 cut(s)	553
BanI	GGYRCC	4 cut(s)	102, 287, 640, 2098
BanII	GRGCYC	3 cut(s)	819, 1607, 2241
BarI	GAAGNNNNNNTAC	2 cut(s)	663, 695
BbsI	GAAGAC	3 cut(s)	50, 1057, 1734
Bbv12I	GWGCWC	3 cut(s)	819, 1663, 2264
BbvI	GCAGC	4 cut(s)	25, 39, 1988, 2058
BccI	CCATC	9 cut(s)	260, 296, 491, 602, 1212, 1262, 1559, 1950, 2226
BceAI	ACGGC	5 cut(s)	305, 309, 972, 1909, 2180
BciT130I	CCWGG	6 cut(s)	34, 771, 1715, 2024, 2094, 2223
BciVI	GTATCC	4 cut(s)	282, 702, 914, 2219
BclI	TGATCA	4 cut(s)	360, 655, 802, 1050
BcnI	CCSGG	1 cut(s)	100
BcoDI	GTCTC	3 cut(s)	209, 1319, 1858
BfaI	CTAG	4 cut(s)	540, 599, 1302, 2283
BfmI	CTRYAG	1 cut(s)	2072
BfoI	RGCGCY	1 cut(s)	82
BfuI	GTATCC	4 cut(s)	282, 702, 914, 2219
BglI	GCCNNNNNGGC	1 cut(s)	2218
BisI	GCNGC	6 cut(s)	14, 28, 1544, 2002, 2072, 2122
BlsI	GCNGC	6 cut(s)	15, 29, 1545, 2003, 2073, 2123
BmcAI	AGTACT	1 cut(s)	872
Bme1390I	CCNGG	7 cut(s)	34, 100, 771, 1715, 2024, 2094, 2223
Bme18I	GGWCC	2 cut(s)	602, 1097
BmeT110I	CYCGRG	1 cut(s)	818
BmgT120I	GGNCC	3 cut(s)	36, 602, 1097
BmrFI	CCNGG	7 cut(s)	34, 100, 771, 1715, 2024, 2094, 2223
BmrI	ACTGGG	2 cut(s)	676, 1507
BmsI	GCATC	8 cut(s)	129, 148, 403, 982, 1169, 1248, 1312, 1450
BmuI	ACTGGG	2 cut(s)	676, 1507
BpiI	GAAGAC	3 cut(s)	50, 1057, 1734
BpmI	CTGGAG	1 cut(s)	2076
BpuEI	CTTGAG	2 cut(s)	440, 1258
BpuMI	CCSGG	1 cut(s)	100
BsaAI	YACGTR	1 cut(s)	977
BsaBI	GATNNNNATC	2 cut(s)	270, 961
BsaHI	GRCGYC	2 cut(s)	2289, 2337
BsaI	GGTCTC	1 cut(s)	1858
BsaJI	CCNNGG	6 cut(s)	291, 781, 1634, 1858, 2093, 2241
BsaWI	WCCGGW	1 cut(s)	105
BsaXI	ACNNNNNCTCC	2 cut(s)	1769, 1799
Bsc4I	CCNNNNNNNGG	6 cut(s)	98, 105, 518, 787, 1396, 1499
Bse1I	ACTGG	4 cut(s)	671, 1219, 1502, 2111
Bse21I	CCTNAGG	1 cut(s)	1152
Bse8I	GATNNNNATC	2 cut(s)	270, 961
BseBI	CCWGG	6 cut(s)	34, 771, 1715, 2024, 2094, 2223
BseDI	CCNNGG	6 cut(s)	291, 781, 1634, 1858, 2093, 2241
BseGI	GGATG	8 cut(s)	144, 1063, 1184, 1204, 1273, 1327, 1465, 2373
BseJI	GATNNNNATC	2 cut(s)	270, 961
BseLI	CCNNNNNNNGG	6 cut(s)	98, 105, 518, 787, 1396, 1499
BseMII	CTCAG	3 cut(s)	195, 387, 753
BseNI	ACTGG	4 cut(s)	671, 1219, 1502, 2111
BseRI	GAGGAG	2 cut(s)	395, 597
BseSI	GKGCMC	3 cut(s)	105, 1663, 2264
BseXI	GCAGC	4 cut(s)	25, 39, 1988, 2058
BseYI	CCCAGC	1 cut(s)	75
BsgI	GTGCAG	1 cut(s)	46
Bsh1236I	CGCG	1 cut(s)	298
BshFI	GGCC	8 cut(s)	37, 238, 622, 987, 1470, 1633, 1896, 2221
BshNI	GGYRCC	4 cut(s)	102, 287, 640, 2098
BsiHKAI	GWGCWC	3 cut(s)	819, 1663, 2264
BsiHKCI	CYCGRG	1 cut(s)	818
BsiSI	CCGG	4 cut(s)	99, 106, 164, 330
BslFI	GGGAC	5 cut(s)	214, 534, 1083, 1850, 2276
BslI	CCNNNNNNNGG	6 cut(s)	98, 105, 518, 787, 1396, 1499
BsmAI	GTCTC	3 cut(s)	209, 1319, 1858
BsmFI	GGGAC	5 cut(s)	214, 534, 1083, 1850, 2276
BsmI	GAATGC	2 cut(s)	1595, 2033
BsnI	GGCC	8 cut(s)	37, 238, 622, 987, 1470, 1633, 1896, 2221
Bso31I	GGTCTC	1 cut(s)	1858
BsoBI	CYCGRG	1 cut(s)	818
Bsp1286I	GDGCHC	6 cut(s)	105, 819, 1607, 1663, 2241, 2264
Bsp143I	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
Bsp19I	CCATGG	1 cut(s)	1634
BspANI	GGCC	8 cut(s)	37, 238, 622, 987, 1470, 1633, 1896, 2221
BspCNI	CTCAG	3 cut(s)	196, 388, 754
BspFNI	CGCG	1 cut(s)	298
BspHI	TCATGA	2 cut(s)	850, 2007
BspMAI	CTGCAG	1 cut(s)	2076
BspPI	GGATC	3 cut(s)	548, 561, 830
BspT107I	GGYRCC	4 cut(s)	102, 287, 640, 2098
BspTNI	GGTCTC	1 cut(s)	1858
BsrBI	CCGCTC	1 cut(s)	44
BsrI	ACTGG	4 cut(s)	671, 1219, 1502, 2111
BssECI	CCNNGG	6 cut(s)	291, 781, 1634, 1858, 2093, 2241
BssMI	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
BssNI	GRCGYC	2 cut(s)	2289, 2337
BssT1I	CCWWGG	4 cut(s)	781, 1634, 1858, 2241
Bst2UI	CCWGG	6 cut(s)	34, 771, 1715, 2024, 2094, 2223
Bst4CI	ACNGT	6 cut(s)	640, 830, 931, 1540, 1670, 1769
Bst6I	CTCTTC	5 cut(s)	116, 510, 541, 1965, 2150
BstACI	GRCGYC	2 cut(s)	2289, 2337
BstAPI	GCANNNNNTGC	1 cut(s)	1460
BstBAI	YACGTR	1 cut(s)	977
BstC8I	GCNNGC	7 cut(s)	210, 596, 620, 970, 1146, 1792, 2185
BstDEI	CTNAG	7 cut(s)	204, 396, 762, 1152, 2191, 2357, 2403
BstDSI	CCRYGG	2 cut(s)	291, 1634
BstF5I	GGATG	8 cut(s)	144, 1063, 1184, 1204, 1273, 1327, 1465, 2373
BstFNI	CGCG	1 cut(s)	298
BstH2I	RGCGCY	1 cut(s)	82
BstHHI	GCGC	5 cut(s)	7, 81, 298, 1150, 1218
BstKTI	GATC	7 cut(s)	363, 556, 658, 805, 825, 1053, 1285
BstMAI	GTCTC	3 cut(s)	209, 1319, 1858
BstMBI	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
BstNI	CCWGG	6 cut(s)	34, 771, 1715, 2024, 2094, 2223
BstNSI	RCATGY	5 cut(s)	449, 728, 1144, 1265, 1489
BstSCI	CCNGG	7 cut(s)	32, 98, 769, 1713, 2022, 2092, 2221
BstSFI	CTRYAG	1 cut(s)	2072
BstSLI	GKGCMC	3 cut(s)	105, 1663, 2264
BstSNI	TACGTA	1 cut(s)	977
BstUI	CGCG	1 cut(s)	298
BstV1I	GCAGC	4 cut(s)	25, 39, 1988, 2058
BstV2I	GAAGAC	3 cut(s)	50, 1057, 1734
BstX2I	RGATCY	1 cut(s)	553
BstYI	RGATCY	1 cut(s)	553
Bsu36I	CCTNAGG	1 cut(s)	1152
BsuI	GTATCC	4 cut(s)	282, 702, 914, 2219
BsuRI	GGCC	8 cut(s)	37, 238, 622, 987, 1470, 1633, 1896, 2221
BtgI	CCRYGG	2 cut(s)	291, 1634
BtsCI	GGATG	8 cut(s)	144, 1063, 1184, 1204, 1273, 1327, 1465, 2373
BtsI	GCAGTG	2 cut(s)	340, 2081
BtsIMutI	CAGTG	6 cut(s)	340, 683, 1495, 1545, 1806, 2081
Cac8I	GCNNGC	7 cut(s)	210, 596, 620, 970, 1146, 1792, 2185
CaiI	CAGNNNCTG	3 cut(s)	33, 203, 1317
CciI	TCATGA	2 cut(s)	850, 2007
CfoI	GCGC	5 cut(s)	7, 81, 298, 1150, 1218
Cfr13I	GGNCC	3 cut(s)	36, 602, 1097
CseI	GACGC	2 cut(s)	236, 2345
Csp6I	GTAC	6 cut(s)	449, 641, 774, 871, 1474, 1560
CviQI	GTAC	6 cut(s)	449, 641, 774, 871, 1474, 1560
DdeI	CTNAG	7 cut(s)	204, 396, 762, 1152, 2191, 2357, 2403
DpnI	GATC	7 cut(s)	362, 555, 657, 804, 824, 1052, 1284
DpnII	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
DraI	TTTAAA	1 cut(s)	810
DrdI	GACNNNNNNGTC	1 cut(s)	427
DseDI	GACNNNNNNGTC	1 cut(s)	427
EaeI	YGGCCR	2 cut(s)	985, 1894
Eam1104I	CTCTTC	5 cut(s)	116, 510, 541, 1965, 2150
EarI	CTCTTC	5 cut(s)	116, 510, 541, 1965, 2150
EciI	GGCGGA	3 cut(s)	151, 227, 2009
Ecl136II	GAGCTC	1 cut(s)	817
Eco105I	TACGTA	1 cut(s)	977
Eco130I	CCWWGG	4 cut(s)	781, 1634, 1858, 2241
Eco147I	AGGCCT	2 cut(s)	1470, 2221
Eco24I	GRGCYC	3 cut(s)	819, 1607, 2241
Eco31I	GGTCTC	1 cut(s)	1858
Eco47I	GGWCC	2 cut(s)	602, 1097
Eco47III	AGCGCT	1 cut(s)	80
Eco53kI	GAGCTC	1 cut(s)	817
Eco57I	CTGAAG	4 cut(s)	707, 859, 1101, 1470
Eco81I	CCTNAGG	1 cut(s)	1152
Eco88I	CYCGRG	1 cut(s)	818
EcoICRI	GAGCTC	1 cut(s)	817
EcoO109I	RGGNCCY	1 cut(s)	602
EcoRI	GAATTC	1 cut(s)	587
EcoRII	CCWGG	6 cut(s)	32, 769, 1713, 2022, 2092, 2221
EcoT14I	CCWWGG	4 cut(s)	781, 1634, 1858, 2241
EcoT22I	ATGCAT	2 cut(s)	726, 1263
EcoT38I	GRGCYC	3 cut(s)	819, 1607, 2241
ErhI	CCWWGG	4 cut(s)	781, 1634, 1858, 2241
FalI	AAGNNNNNCTT	4 cut(s)	462, 494, 2350, 2382
FaqI	GGGAC	5 cut(s)	214, 534, 1083, 1850, 2276
FauI	CCCGC	3 cut(s)	71, 185, 299
FbaI	TGATCA	4 cut(s)	360, 655, 802, 1050
FblI	GTMKAC	3 cut(s)	231, 1230, 2247
Fnu4HI	GCNGC	6 cut(s)	14, 28, 1544, 2002, 2072, 2122
FokI	GGATG	8 cut(s)	151, 1070, 1191, 1191, 1280, 1334, 1472, 2360
FriOI	GRGCYC	3 cut(s)	819, 1607, 2241
Fsp4HI	GCNGC	6 cut(s)	14, 28, 1544, 2002, 2072, 2122
FspBI	CTAG	4 cut(s)	540, 599, 1302, 2283
GlaI	GCGC	5 cut(s)	6, 80, 297, 1149, 1217
GluI	GCNGC	6 cut(s)	14, 28, 1544, 2002, 2072, 2122
GsaI	CCCAGC	1 cut(s)	79
GsuI	CTGGAG	1 cut(s)	2076
HaeII	RGCGCY	1 cut(s)	82
HaeIII	GGCC	8 cut(s)	37, 238, 622, 987, 1470, 1633, 1896, 2221
HapII	CCGG	4 cut(s)	99, 106, 164, 330
HgaI	GACGC	2 cut(s)	236, 2345
HhaI	GCGC	5 cut(s)	7, 81, 298, 1150, 1218
Hin1I	GRCGYC	2 cut(s)	2289, 2337
Hin6I	GCGC	5 cut(s)	5, 79, 296, 1148, 1216
HinP1I	GCGC	5 cut(s)	5, 79, 296, 1148, 1216
HincII	GTYRAC	5 cut(s)	232, 313, 1365, 1402, 2248
HindII	GTYRAC	5 cut(s)	232, 313, 1365, 1402, 2248
HindIII	AAGCTT	1 cut(s)	1439
HpaI	GTTAAC	1 cut(s)	313
HpaII	CCGG	4 cut(s)	99, 106, 164, 330
HphI	GGTGA	7 cut(s)	167, 187, 908, 936, 1060, 1367, 1895
Hpy188I	TCNGA	8 cut(s)	120, 265, 276, 373, 1513, 1984, 1994, 2113
Hpy99I	CGWCG	3 cut(s)	252, 1670, 2339
HpyAV	CCTTC	7 cut(s)	172, 654, 665, 1076, 1346, 1436, 1616
HpyCH4III	ACNGT	6 cut(s)	640, 830, 931, 1540, 1670, 1769
HpyCH4IV	ACGT	3 cut(s)	976, 1036, 2289
HpyF3I	CTNAG	7 cut(s)	204, 396, 762, 1152, 2191, 2357, 2403
HpySE526I	ACGT	3 cut(s)	976, 1036, 2289
Hsp92I	GRCGYC	2 cut(s)	2289, 2337
HspAI	GCGC	5 cut(s)	5, 79, 296, 1148, 1216
KpnI	GGTACC	1 cut(s)	644
Ksp22I	TGATCA	4 cut(s)	360, 655, 802, 1050
KspAI	GTTAAC	1 cut(s)	313
Kzo9I	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
LmnI	GCTCC	6 cut(s)	49, 166, 814, 1602, 2095, 2236
Lsp1109I	GCAGC	4 cut(s)	25, 39, 1988, 2058
LweI	GCATC	8 cut(s)	129, 148, 403, 982, 1169, 1248, 1312, 1450
MaeI	CTAG	4 cut(s)	540, 599, 1302, 2283
MaeII	ACGT	3 cut(s)	976, 1036, 2289
MalI	GATC	7 cut(s)	362, 555, 657, 804, 824, 1052, 1284
MbiI	CCGCTC	1 cut(s)	44
MboI	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
MfeI	CAATTG	1 cut(s)	1195
MflI	RGATCY	1 cut(s)	553
MhlI	GDGCHC	6 cut(s)	105, 819, 1607, 1663, 2241, 2264
MlyI	GAGTC	6 cut(s)	286, 527, 1756, 1982, 2042, 2141
MmeI	TCCRAC	1 cut(s)	846
Mph1103I	ATGCAT	2 cut(s)	726, 1263
MseI	TTAA	9 cut(s)	312, 651, 809, 839, 1329, 1679, 1944, 2045, 2313
MslI	CAYNNNNRTG	6 cut(s)	528, 729, 1035, 1266, 1799, 2085
MspA1I	CMGCKG	1 cut(s)	1079
MspI	CCGG	4 cut(s)	99, 106, 164, 330
MspR9I	CCNGG	7 cut(s)	34, 100, 771, 1715, 2024, 2094, 2223
MunI	CAATTG	1 cut(s)	1195
Mva1269I	GAATGC	2 cut(s)	1595, 2033
MvaI	CCWGG	6 cut(s)	34, 771, 1715, 2024, 2094, 2223
MvnI	CGCG	1 cut(s)	298
NciI	CCSGG	1 cut(s)	100
NcoI	CCATGG	1 cut(s)	1634
NdeII	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
NmuCI	GTSAC	7 cut(s)	346, 896, 1355, 1532, 2034, 2197, 2285
NsiI	ATGCAT	2 cut(s)	726, 1263
NspI	RCATGY	5 cut(s)	449, 728, 1144, 1265, 1489
PagI	TCATGA	2 cut(s)	850, 2007
PceI	AGGCCT	2 cut(s)	1470, 2221
PctI	GAATGC	2 cut(s)	1595, 2033
PfeI	GAWTC	4 cut(s)	109, 403, 749, 2399
PkrI	GCNGC	6 cut(s)	15, 29, 1545, 2003, 2073, 2123
PleI	GAGTC	6 cut(s)	285, 527, 1755, 1981, 2041, 2141
PpsI	GAGTC	6 cut(s)	285, 527, 1755, 1981, 2041, 2141
Ppu21I	YACGTR	1 cut(s)	977
PpuMI	RGGWCCY	1 cut(s)	602
PshBI	ATTAAT	2 cut(s)	1329, 1944
Psp124BI	GAGCTC	1 cut(s)	819
Psp5II	RGGWCCY	1 cut(s)	602
Psp6I	CCWGG	6 cut(s)	32, 769, 1713, 2022, 2092, 2221
PspFI	CCCAGC	1 cut(s)	75
PspGI	CCWGG	6 cut(s)	32, 769, 1713, 2022, 2092, 2221
PspPI	GGNCC	3 cut(s)	36, 602, 1097
PspPPI	RGGWCCY	1 cut(s)	602
PstI	CTGCAG	1 cut(s)	2076
PstNI	CAGNNNCTG	3 cut(s)	33, 203, 1317
PsuI	RGATCY	1 cut(s)	553
PvuII	CAGCTG	1 cut(s)	1079
RsaI	GTAC	6 cut(s)	450, 642, 775, 872, 1475, 1561
RsaNI	GTAC	6 cut(s)	449, 641, 774, 871, 1474, 1560
RseI	CAYNNNNRTG	6 cut(s)	528, 729, 1035, 1266, 1799, 2085
SacI	GAGCTC	1 cut(s)	819
SalI	GTCGAC	2 cut(s)	230, 2246
SaqAI	TTAA	9 cut(s)	312, 651, 809, 839, 1329, 1679, 1944, 2045, 2313
SatI	GCNGC	6 cut(s)	14, 28, 1544, 2002, 2072, 2122
Sau3AI	GATC	7 cut(s)	360, 553, 655, 802, 822, 1050, 1282
Sau96I	GGNCC	3 cut(s)	36, 602, 1097
ScaI	AGTACT	1 cut(s)	872
SchI	GAGTC	6 cut(s)	286, 527, 1756, 1982, 2042, 2141
ScrFI	CCNGG	7 cut(s)	34, 100, 771, 1715, 2024, 2094, 2223
SduI	GDGCHC	6 cut(s)	105, 819, 1607, 1663, 2241, 2264
SfaNI	GCATC	8 cut(s)	129, 148, 403, 982, 1169, 1248, 1312, 1450
SfcI	CTRYAG	1 cut(s)	2072
SinI	GGWCC	2 cut(s)	602, 1097
SmiMI	CAYNNNNRTG	6 cut(s)	528, 729, 1035, 1266, 1799, 2085
SmlI	CTYRAG	2 cut(s)	455, 1237
SmoI	CTYRAG	2 cut(s)	455, 1237
SnaBI	TACGTA	1 cut(s)	977
SseBI	AGGCCT	2 cut(s)	1470, 2221
SspMI	CTAG	4 cut(s)	540, 599, 1302, 2283
SstI	GAGCTC	1 cut(s)	819
StuI	AGGCCT	2 cut(s)	1470, 2221
StyD4I	CCNGG	7 cut(s)	32, 98, 769, 1713, 2022, 2092, 2221
StyI	CCWWGG	4 cut(s)	781, 1634, 1858, 2241
TaaI	ACNGT	6 cut(s)	640, 830, 931, 1540, 1670, 1769
TaiI	ACGT	3 cut(s)	979, 1039, 2292
TaqI	TCGA	6 cut(s)	231, 419, 926, 1954, 2247, 2334
TatI	WGTACW	2 cut(s)	448, 870
TauI	GCSGC	2 cut(s)	1546, 2124
TfiI	GAWTC	4 cut(s)	109, 403, 749, 2399
Tru1I	TTAA	9 cut(s)	312, 651, 809, 839, 1329, 1679, 1944, 2045, 2313
Tru9I	TTAA	9 cut(s)	312, 651, 809, 839, 1329, 1679, 1944, 2045, 2313
TscAI	CASTG	6 cut(s)	340, 690, 1502, 1545, 1806, 2081
TseFI	GTSAC	7 cut(s)	346, 896, 1355, 1532, 2034, 2197, 2285
TseI	GCWGC	4 cut(s)	13, 27, 2001, 2071
Tsp45I	GTSAC	7 cut(s)	346, 896, 1355, 1532, 2034, 2197, 2285
TspDTI	ATGAA	7 cut(s)	732, 762, 951, 1017, 1104, 1418, 1902
TspGWI	ACGGA	4 cut(s)	44, 270, 334, 1755
TspRI	CASTG	6 cut(s)	340, 690, 1502, 1545, 1806, 2081
VneI	GTGCAC	2 cut(s)	1659, 2260
VpaK11BI	GGWCC	2 cut(s)	602, 1097
VspI	ATTAAT	2 cut(s)	1329, 1944
XapI	RAATTY	5 cut(s)	491, 587, 910, 1831, 2015
XbaI	TCTAGA	1 cut(s)	539
XceI	RCATGY	5 cut(s)	449, 728, 1144, 1265, 1489
XmiI	GTMKAC	3 cut(s)	231, 1230, 2247
XspI	CTAG	4 cut(s)	540, 599, 1302, 2283
ZraI	GACGTC	1 cut(s)	2290
ZrmI	AGTACT	1 cut(s)	872
Zsp2I	ATGCAT	2 cut(s)	726, 1263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.