Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

14698956 .. 14708504

Gene Structure 9549 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ37040

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGAGCACCTAACCACTTGGTCCAGTGCTCCCAGACTCCACTCTCACCACCACCGCCTCTTTGCTTCCAATGCAAGGCTCCGGAGCCGAAGCCCCAACCGTCTCAATTGGCGAATGCTAGGGCTTCGGAGAGGCGGTGCTCCGTCGGCGGTGAAGGCAAGCGACGGAGGCGCGGCGGTGGATACCGCGGTGGCCGAGAGGCCGAAACTGCGTCGTTTTCAGGTCTCCGAAGGTCATCCGACTCCCTTTGGCGCCACGCTCCGCGACGGTGGGGTTAACTTCGCTGTCTACGCCGGCAATGCCGTTTCCGCCACTCTCTGTTTGATCACTCTCGCCGATTTAGAGCAGGATAAAGTTACTGAGCAGATCCCTCTTGATCCTGTGAACAATAAGACTGGTAATGTGTGGCATGTATTTCTAACTGGAGATGTCAAAGATATCCTGTATGGATACAAATTCGACGGCAAGTTTTCTCCGCAAGAGGGACACTACTATGATTCGTCAAAGATTGTATTGGATCCTTATGCAAAGGCAGTTATACGCCGAGGTGAATTTGGGAGTCTAGGTCCTGATGGTAATTGCTGGCCTCAAATGGCCTGCACGGTACCTTCTTTTGATGATAAGTTCAACTGGGAAGGAGATTTACCTCTGAAGTATCCACAAAAAGATCTTATCATATATGAAATGCATGTGCGAGGTTTTACAAGGCATGAATCGAGCATGACTGAATTGCCTGGTACATACTGTGGTTTGGTGGAGAAGCTTGATCACTTAAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCATGTCATGAGTTCAATGAACTGGAGTACTTTGGCTACAATTCTGTCTTGGGTGACTACAAGGTAAATTTTTGGGGATATTCAACTGTCAGTTACTTTTCACCAATGATAAGATATTCATCCGCTGGCACACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTGTTAAAGAAGCACATAGACGTGGAATTGAGGTGATCATGGATGTTGTTTTCAATCACACAGCTGAAGGGAATGAAAATGGTCCCATTTTGTCTTTTAGAGGTGCTGACAACAGTGTCTATTACATGCTCGCCCCTAAGGGAGAGTTTTATAATTATTCAGGGTGTGGTAACACATTCAATTGCAACCATCCTGTTGTGCGACAATTCATAGTAGATTGCTTGAGATATTGGGTAACAGAGATGCACGTGGATGGGTTCCGCTTTGATCTTGCTTCTATTATGACCAGGGGTACCAGTCTCTGGGATTCAACTAATGTATACGGGAGTCTGATAGAAGGTGACTTGTTGACAACTGGAACCCCTCTTGCTAGCCCTCCATTGATTGACATGATTAGTAATGACCCAATACTTCATGGAGTGAAGCTGATAGCTGAAGCTTGGGATGCAGGAGGCCTGTACCAACTTGGCATGTTTCCTCACTGGGGTATTTGGTCAGAATGGAACGGGAAGTATCGTGACACAGTGCGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTTTGGCCGAATGCCTTTGTGGGAGCCCTAATTTGTACCAGGAAGGGGGAGGGAGACCATGGAACAGTATCAATTTTATTTGTGCACACGATGGTTTTACTTTAGCTGATTTAGTAACCTATAACAACAAACATAACCTGGCAAACGGAGAAGACAACAATGACGGAGAGAATCATAATAATAGCTGGAACTGCGGACAGGAGGGAGAATTCACAAGCATTTCAGTGAAAAAACTGAGGAAGCGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTTTCCCAAGGTGTTCCAATGATATATATGGGTGATGAGTATGGTCATACAAAAGGGGGGAACAACAACACATATTGCCATGATAATTATATTAATTACTTCCGGTGGGATAAGAAGGAAGAGTCTTCATCAGATTTTTTCAGATTTTGCTGTCTTATGACCAAATTCCGCCAGGAATGTGAGTCACTTGGTTTGAATGACTTCCCAACAGCAGAGAGACTGCAGTGGCATGGTCTTTCTCCTGGGCAGCCAGACTGGTCTAAAACAAGCCGCTTTGTGGCCTTTACTCTGGTTGACTCAGTGAAGAGAGAGATCTACATTGCCTTCAATGCCAGTCATTTACCCGTGAACATTTCACTGCCAGAGAGGCCTGGATACAGATGGGAACCCTTGGTAGACACTAGCAAGTCTTCACCCTTTGACTTCCTATCTAGTGGCCTCCCACAAAGAGATATTGCAGTTAAACAGTATGCTCACTTTCTTGATGCCAATTTATACCCGATGCTAAGTTATTCTTCAATCATCCTATTGCTCGCTCCAGTCGAAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

790

Amino Acids

89.14

Weight (kDa)

5.96

Isoelectric Point (pI)

42.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	82 - 176	6.3e-15	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	240 - 350	3.5e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	681 - 782	4.3e-45	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1131
AasI	GACNNNNNNGTC	1 cut(s)	1094
Acc65I	GGTACC	2 cut(s)	604, 1271
AccB1I	GGYRCC	3 cut(s)	251, 604, 1271
AccB7I	CCANNNNNTGG	1 cut(s)	1281
AccI	GTMKAC	3 cut(s)	288, 1299, 2214
AccII	CGCG	3 cut(s)	173, 188, 264
AccIII	TCCGGA	1 cut(s)	81
AclWI	GGATC	5 cut(s)	361, 371, 512, 525, 794
AcoI	YGGCCR	3 cut(s)	192, 949, 1548
AcsI	RAATTY	6 cut(s)	455, 551, 874, 1751, 1798, 1982
AcuI	CTGAAG	3 cut(s)	671, 1065, 1434
AcvI	CACGTG	1 cut(s)	1228
AcyI	GRCGYC	1 cut(s)	252
AfaI	GTAC	7 cut(s)	606, 739, 836, 1273, 1439, 1525, 1580
AfiI	CCNNNNNNNGG	6 cut(s)	75, 482, 1281, 1463, 1588, 2095
AflIII	ACRYGT	1 cut(s)	938
AgsI	TTSAA	9 cut(s)	628, 823, 891, 1033, 1159, 1290, 2014, 2146, 2337
AjiI	CACGTC	1 cut(s)	1001
AjnI	CCWGG	7 cut(s)	733, 1265, 1581, 1680, 1989, 2059, 2188
AluBI	AGCT	7 cut(s)	763, 1043, 1405, 1412, 1418, 1649, 1728
AluI	AGCT	7 cut(s)	763, 1043, 1405, 1412, 1418, 1649, 1728
Alw21I	GWGCWC	4 cut(s)	9, 31, 142, 1630
Alw26I	GTCTC	5 cut(s)	107, 229, 1283, 1591, 2029
Alw44I	GTGCAC	1 cut(s)	1626
AlwI	GGATC	5 cut(s)	361, 371, 512, 525, 794
AlwNI	CAGNNNCTG	1 cut(s)	1281
Aor13HI	TCCGGA	1 cut(s)	81
ApaLI	GTGCAC	1 cut(s)	1626
ApeKI	GCWGC	1 cut(s)	2065
ApoI	RAATTY	6 cut(s)	455, 551, 874, 1751, 1798, 1982
AseI	ATTAAT	1 cut(s)	1911
Asp700I	GAANNNNTTC	1 cut(s)	2018
Asp718I	GGTACC	2 cut(s)	604, 1271
AspLEI	GCGC	2 cut(s)	173, 254
AspS9I	GGNCC	3 cut(s)	21, 566, 1061
AsuHPI	GGTGA	9 cut(s)	38, 163, 560, 872, 900, 1024, 1331, 1862, 2223
AsuNHI	GCTAGC	1 cut(s)	1349
AvaII	GGWCC	3 cut(s)	21, 566, 1061
AxyI	CCTNAGG	1 cut(s)	1116
BaeGI	GKGCMC	1 cut(s)	1630
BaeI	ACNNNNGTAYC	2 cut(s)	174, 207
BamHI	GGATCC	1 cut(s)	517
BanI	GGYRCC	3 cut(s)	251, 604, 1271
BanII	GRGCYC	1 cut(s)	1571
BarI	GAAGNNNNNNTAC	2 cut(s)	627, 659
BbrPI	CACGTG	1 cut(s)	1228
BbsI	GAAGAC	3 cut(s)	1701, 1935, 2220
Bbv12I	GWGCWC	4 cut(s)	9, 31, 142, 1630
BbvI	GCAGC	1 cut(s)	2077
BccI	CCATC	6 cut(s)	566, 1176, 1226, 1523, 1628, 2193
BceAI	ACGGC	3 cut(s)	287, 478, 936
BciT130I	CCWGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
BciVI	GTATCC	4 cut(s)	175, 443, 666, 2186
BclI	TGATCA	3 cut(s)	324, 766, 1014
BcoDI	GTCTC	5 cut(s)	107, 229, 1283, 1591, 2029
BfaI	CTAG	5 cut(s)	119, 563, 1350, 2220, 2250
BfmI	CTRYAG	1 cut(s)	2039
BfoI	RGCGCY	1 cut(s)	255
BfuI	GTATCC	4 cut(s)	175, 443, 666, 2186
BglI	GCCNNNNNGGC	1 cut(s)	2185
BglII	AGATCT	2 cut(s)	667, 2130
BisI	GCNGC	4 cut(s)	174, 1508, 2066, 2089
BlsI	GCNGC	4 cut(s)	175, 1509, 2067, 2090
BmcAI	AGTACT	1 cut(s)	836
Bme1390I	CCNGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
Bme18I	GGWCC	3 cut(s)	21, 566, 1061
BmgBI	CACGTC	1 cut(s)	1001
BmgT120I	GGNCC	3 cut(s)	21, 566, 1061
BmrFI	CCNGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
BmrI	ACTGGG	2 cut(s)	640, 1471
BmsI	GCATC	6 cut(s)	946, 1212, 1414, 2293, 2310, 2356
BmtI	GCTAGC	1 cut(s)	1353
BmuI	ACTGGG	2 cut(s)	640, 1471
BpiI	GAAGAC	3 cut(s)	1701, 1935, 2220
BpmI	CTGGAG	3 cut(s)	444, 851, 2340
BpuEI	CTTGAG	1 cut(s)	1222
BsaAI	YACGTR	2 cut(s)	941, 1228
BsaBI	GATNNNNATC	1 cut(s)	925
BsaHI	GRCGYC	1 cut(s)	252
BsaI	GGTCTC	2 cut(s)	229, 1591
BsaJI	CCNNGG	7 cut(s)	186, 544, 1266, 1601, 1825, 2060, 2208
BsaWI	WCCGGW	2 cut(s)	81, 1920
BsaXI	ACNNNNNCTCC	4 cut(s)	121, 151, 550, 580
Bsc4I	CCNNNNNNNGG	6 cut(s)	75, 482, 1281, 1463, 1588, 2095
Bse118I	RCCGGY	1 cut(s)	293
Bse21I	CCTNAGG	1 cut(s)	1116
Bse3DI	GCAATG	2 cut(s)	304, 2136
Bse8I	GATNNNNATC	1 cut(s)	925
BseAI	TCCGGA	1 cut(s)	81
BseBI	CCWGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
BseDI	CCNNGG	7 cut(s)	186, 544, 1266, 1601, 1825, 2060, 2208
BseGI	GGATG	7 cut(s)	235, 926, 1027, 1168, 1237, 1429, 2340
BseJI	GATNNNNATC	1 cut(s)	925
BseLI	CCNNNNNNNGG	6 cut(s)	75, 482, 1281, 1463, 1588, 2095
BseMI	GCAATG	2 cut(s)	304, 2136
BseMII	CTCAG	3 cut(s)	351, 1769, 2130
BseSI	GKGCMC	1 cut(s)	1630
BseXI	GCAGC	1 cut(s)	2077
BsgI	GTGCAG	1 cut(s)	583
Bsh1236I	CGCG	3 cut(s)	173, 188, 264
BshNI	GGYRCC	3 cut(s)	251, 604, 1271
BsiHKAI	GWGCWC	4 cut(s)	9, 31, 142, 1630
BsiSI	CCGG	3 cut(s)	82, 294, 1921
BslFI	GGGAC	2 cut(s)	498, 1047
BslI	CCNNNNNNNGG	6 cut(s)	75, 482, 1281, 1463, 1588, 2095
BsmAI	GTCTC	5 cut(s)	107, 229, 1283, 1591, 2029
BsmBI	CGTCTC	1 cut(s)	107
BsmFI	GGGAC	2 cut(s)	498, 1047
BsmI	GAATGC	2 cut(s)	120, 1559
Bso31I	GGTCTC	2 cut(s)	229, 1591
Bsp1286I	GDGCHC	5 cut(s)	9, 31, 142, 1571, 1630
Bsp13I	TCCGGA	1 cut(s)	81
Bsp19I	CCATGG	1 cut(s)	1601
BspCNI	CTCAG	3 cut(s)	352, 1770, 2129
BspEI	TCCGGA	1 cut(s)	81
BspFNI	CGCG	3 cut(s)	173, 188, 264
BspHI	TCATGA	1 cut(s)	814
BspMAI	CTGCAG	1 cut(s)	2043
BspOI	GCTAGC	1 cut(s)	1353
BspPI	GGATC	5 cut(s)	361, 371, 512, 525, 794
BspT107I	GGYRCC	3 cut(s)	251, 604, 1271
BspTNI	GGTCTC	2 cut(s)	229, 1591
BsrDI	GCAATG	2 cut(s)	304, 2136
BsrFI	RCCGGY	1 cut(s)	293
BssAI	RCCGGY	1 cut(s)	293
BssECI	CCNNGG	7 cut(s)	186, 544, 1266, 1601, 1825, 2060, 2208
BssNAI	GTATAC	1 cut(s)	1300
BssNI	GRCGYC	1 cut(s)	252
BssT1I	CCWWGG	3 cut(s)	1601, 1825, 2208
Bst1107I	GTATAC	1 cut(s)	1300
Bst2UI	CCWGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
Bst6I	CTCTTC	2 cut(s)	1932, 2117
BstACI	GRCGYC	1 cut(s)	252
BstBAI	YACGTR	2 cut(s)	941, 1228
BstC8I	GCNNGC	8 cut(s)	160, 295, 584, 598, 934, 1110, 1351, 2352
BstDEI	CTNAG	6 cut(s)	360, 1116, 1778, 2116, 2324, 2370
BstDSI	CCRYGG	2 cut(s)	186, 1601
BstF5I	GGATG	7 cut(s)	235, 926, 1027, 1168, 1237, 1429, 2340
BstFNI	CGCG	3 cut(s)	173, 188, 264
BstH2I	RGCGCY	1 cut(s)	255
BstHHI	GCGC	2 cut(s)	173, 254
BstMAI	GTCTC	5 cut(s)	107, 229, 1283, 1591, 2029
BstNI	CCWGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
BstNSI	RCATGY	4 cut(s)	413, 692, 1108, 1453
BstSCI	CCNGG	7 cut(s)	733, 1265, 1581, 1680, 1989, 2059, 2188
BstSFI	CTRYAG	1 cut(s)	2039
BstSLI	GKGCMC	1 cut(s)	1630
BstUI	CGCG	3 cut(s)	173, 188, 264
BstV1I	GCAGC	1 cut(s)	2077
BstV2I	GAAGAC	3 cut(s)	1701, 1935, 2220
BstX2I	RGATCY	4 cut(s)	366, 517, 667, 2130
BstYI	RGATCY	4 cut(s)	366, 517, 667, 2130
BstZ17I	GTATAC	1 cut(s)	1300
Bsu36I	CCTNAGG	1 cut(s)	1116
BsuI	GTATCC	4 cut(s)	175, 443, 666, 2186
BtgI	CCRYGG	2 cut(s)	186, 1601
BtrI	CACGTC	1 cut(s)	1001
BtsCI	GGATG	7 cut(s)	235, 926, 1027, 1168, 1237, 1429, 2340
BtsI	GCAGTG	2 cut(s)	2048, 2174
BtsIMutI	CAGTG	8 cut(s)	31, 1099, 1459, 1509, 1773, 2048, 2124, 2174
Cac8I	GCNNGC	8 cut(s)	160, 295, 584, 598, 934, 1110, 1351, 2352
CaiI	CAGNNNCTG	1 cut(s)	1281
CciI	TCATGA	1 cut(s)	814
CfoI	GCGC	2 cut(s)	173, 254
Cfr10I	RCCGGY	1 cut(s)	293
Cfr13I	GGNCC	3 cut(s)	21, 566, 1061
Cfr42I	CCGCGG	1 cut(s)	189
CseI	GACGC	1 cut(s)	200
Csp6I	GTAC	7 cut(s)	605, 738, 835, 1272, 1438, 1524, 1579
CviQI	GTAC	7 cut(s)	605, 738, 835, 1272, 1438, 1524, 1579
DdeI	CTNAG	6 cut(s)	360, 1116, 1778, 2116, 2324, 2370
DinI	GGCGCC	1 cut(s)	253
DrdI	GACNNNNNNGTC	1 cut(s)	1094
DseDI	GACNNNNNNGTC	1 cut(s)	1094
EaeI	YGGCCR	3 cut(s)	192, 949, 1548
Eam1104I	CTCTTC	2 cut(s)	1932, 2117
EarI	CTCTTC	2 cut(s)	1932, 2117
EciI	GGCGGA	2 cut(s)	298, 1976
Eco130I	CCWWGG	3 cut(s)	1601, 1825, 2208
Eco147I	AGGCCT	2 cut(s)	1434, 2188
Eco24I	GRGCYC	1 cut(s)	1571
Eco31I	GGTCTC	2 cut(s)	229, 1591
Eco32I	GATATC	1 cut(s)	439
Eco47I	GGWCC	3 cut(s)	21, 566, 1061
Eco57I	CTGAAG	3 cut(s)	671, 1065, 1434
Eco72I	CACGTG	1 cut(s)	1228
Eco81I	CCTNAGG	1 cut(s)	1116
EcoO109I	RGGNCCY	1 cut(s)	566
EcoRI	GAATTC	1 cut(s)	1751
EcoRII	CCWGG	7 cut(s)	733, 1265, 1581, 1680, 1989, 2059, 2188
EcoRV	GATATC	1 cut(s)	439
EcoT14I	CCWWGG	3 cut(s)	1601, 1825, 2208
EcoT22I	ATGCAT	1 cut(s)	690
EcoT38I	GRGCYC	1 cut(s)	1571
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
ErhI	CCWWGG	3 cut(s)	1601, 1825, 2208
Esp3I	CGTCTC	1 cut(s)	107
FalI	AAGNNNNNCTT	2 cut(s)	2317, 2349
FaqI	GGGAC	2 cut(s)	498, 1047
FbaI	TGATCA	3 cut(s)	324, 766, 1014
FblI	GTMKAC	3 cut(s)	288, 1299, 2214
Fnu4HI	GCNGC	4 cut(s)	174, 1508, 2066, 2089
FokI	GGATG	7 cut(s)	222, 913, 1034, 1155, 1244, 1436, 2327
FriOI	GRGCYC	1 cut(s)	1571
Fsp4HI	GCNGC	4 cut(s)	174, 1508, 2066, 2089
FspBI	CTAG	5 cut(s)	119, 563, 1350, 2220, 2250
GlaI	GCGC	2 cut(s)	172, 253
GluI	GCNGC	4 cut(s)	174, 1508, 2066, 2089
GsuI	CTGGAG	3 cut(s)	444, 851, 2340
HaeII	RGCGCY	1 cut(s)	255
HapII	CCGG	3 cut(s)	82, 294, 1921
HgaI	GACGC	1 cut(s)	200
HhaI	GCGC	2 cut(s)	173, 254
Hin1I	GRCGYC	1 cut(s)	252
Hin6I	GCGC	2 cut(s)	171, 252
HinP1I	GCGC	2 cut(s)	171, 252
HincII	GTYRAC	3 cut(s)	277, 1329, 2113
HindII	GTYRAC	3 cut(s)	277, 1329, 2113
HindIII	AAGCTT	2 cut(s)	761, 1416
HpaI	GTTAAC	1 cut(s)	277
HpaII	CCGG	3 cut(s)	82, 294, 1921
HphI	GGTGA	9 cut(s)	38, 163, 560, 872, 900, 1024, 1331, 1862, 2223
Hpy166II	GTNNAC	9 cut(s)	277, 289, 385, 1300, 1329, 1628, 2113, 2167, 2215
Hpy188I	TCNGA	8 cut(s)	129, 229, 240, 651, 1311, 1477, 1951, 1961
Hpy188III	TCNNGA	6 cut(s)	82, 374, 569, 815, 1496, 2300
Hpy8I	GTNNAC	9 cut(s)	277, 289, 385, 1300, 1329, 1628, 2113, 2167, 2215
Hpy99I	CGWCG	5 cut(s)	148, 167, 216, 269, 464
HpyAV	CCTTC	9 cut(s)	148, 224, 618, 629, 1040, 1310, 1580, 1927, 2152
HpyCH4IV	ACGT	3 cut(s)	940, 1000, 1227
HpyF3I	CTNAG	6 cut(s)	360, 1116, 1778, 2116, 2324, 2370
HpySE526I	ACGT	3 cut(s)	940, 1000, 1227
Hsp92I	GRCGYC	1 cut(s)	252
HspAI	GCGC	2 cut(s)	171, 252
KasI	GGCGCC	1 cut(s)	251
Kpn2I	TCCGGA	1 cut(s)	81
KpnI	GGTACC	2 cut(s)	608, 1275
KroI	GCCGGC	1 cut(s)	293
KroNI	GCCGGC	1 cut(s)	295
Ksp22I	TGATCA	3 cut(s)	324, 766, 1014
KspAI	GTTAAC	1 cut(s)	277
KspI	CCGCGG	1 cut(s)	189
LmnI	GCTCC	8 cut(s)	4, 34, 84, 84, 145, 264, 1566, 2359
Lsp1109I	GCAGC	1 cut(s)	2077
LweI	GCATC	6 cut(s)	946, 1212, 1414, 2293, 2310, 2356
MaeI	CTAG	5 cut(s)	119, 563, 1350, 2220, 2250
MaeII	ACGT	3 cut(s)	940, 1000, 1227
MaeIII	GTNAC	9 cut(s)	355, 860, 899, 1148, 1213, 1319, 1496, 1657, 2001
MboII	GAAGA	6 cut(s)	1706, 1935, 1949, 2134, 2220, 2325
MfeI	CAATTG	2 cut(s)	106, 1159
MflI	RGATCY	4 cut(s)	366, 517, 667, 2130
MhlI	GDGCHC	5 cut(s)	9, 31, 142, 1571, 1630
Mly113I	GGCGCC	1 cut(s)	252
MlyI	GAGTC	7 cut(s)	30, 235, 568, 1315, 1949, 2009, 2108
MmeI	TCCRAC	1 cut(s)	263
Mph1103I	ATGCAT	1 cut(s)	690
MroI	TCCGGA	1 cut(s)	81
MroNI	GCCGGC	1 cut(s)	293
MroXI	GAANNNNTTC	1 cut(s)	2018
MseI	TTAA	6 cut(s)	276, 773, 803, 984, 1911, 2280
MslI	CAYNNNNRTG	4 cut(s)	492, 999, 1230, 1766
MspA1I	CMGCKG	3 cut(s)	188, 932, 1043
MspI	CCGG	3 cut(s)	82, 294, 1921
MspR9I	CCNGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
MunI	CAATTG	2 cut(s)	106, 1159
Mva1269I	GAATGC	2 cut(s)	120, 1559
MvaI	CCWGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
MvnI	CGCG	3 cut(s)	173, 188, 264
NaeI	GCCGGC	1 cut(s)	295
NarI	GGCGCC	1 cut(s)	252
NcoI	CCATGG	1 cut(s)	1601
NgoMIV	GCCGGC	1 cut(s)	293
NheI	GCTAGC	1 cut(s)	1349
NmeAIII	GCCGAG	2 cut(s)	220, 569
NmuCI	GTSAC	4 cut(s)	860, 1319, 1496, 2001
NsiI	ATGCAT	1 cut(s)	690
NspI	RCATGY	4 cut(s)	413, 692, 1108, 1453
PagI	TCATGA	1 cut(s)	814
PceI	AGGCCT	2 cut(s)	1434, 2188
PcsI	WCGNNNNNNNCGW	1 cut(s)	2358
PctI	GAATGC	2 cut(s)	120, 1559
PdiI	GCCGGC	1 cut(s)	295
PdmI	GAANNNNTTC	1 cut(s)	2018
PfeI	GAWTC	4 cut(s)	497, 713, 1286, 1714
PflMI	CCANNNNNTGG	1 cut(s)	1281
PkrI	GCNGC	4 cut(s)	175, 1509, 2067, 2090
PleI	GAGTC	7 cut(s)	30, 235, 567, 1314, 1948, 2008, 2108
PluTI	GGCGCC	1 cut(s)	255
PmaCI	CACGTG	1 cut(s)	1228
PmlI	CACGTG	1 cut(s)	1228
PpsI	GAGTC	7 cut(s)	30, 235, 567, 1314, 1948, 2008, 2108
Ppu21I	YACGTR	2 cut(s)	941, 1228
PpuMI	RGGWCCY	1 cut(s)	566
PshBI	ATTAAT	1 cut(s)	1911
PsiI	TTATAA	1 cut(s)	1131
Psp5II	RGGWCCY	1 cut(s)	566
Psp6I	CCWGG	7 cut(s)	733, 1265, 1581, 1680, 1989, 2059, 2188
PspCI	CACGTG	1 cut(s)	1228
PspGI	CCWGG	7 cut(s)	733, 1265, 1581, 1680, 1989, 2059, 2188
PspPI	GGNCC	3 cut(s)	21, 566, 1061
PspPPI	RGGWCCY	1 cut(s)	566
PstI	CTGCAG	1 cut(s)	2043
PstNI	CAGNNNCTG	1 cut(s)	1281
PsuI	RGATCY	4 cut(s)	366, 517, 667, 2130
PvuII	CAGCTG	1 cut(s)	1043
RsaI	GTAC	7 cut(s)	606, 739, 836, 1273, 1439, 1525, 1580
RsaNI	GTAC	7 cut(s)	605, 738, 835, 1272, 1438, 1524, 1579
RseI	CAYNNNNRTG	4 cut(s)	492, 999, 1230, 1766
SacII	CCGCGG	1 cut(s)	189
SaqAI	TTAA	6 cut(s)	276, 773, 803, 984, 1911, 2280
SatI	GCNGC	4 cut(s)	174, 1508, 2066, 2089
Sau96I	GGNCC	3 cut(s)	21, 566, 1061
ScaI	AGTACT	1 cut(s)	836
SchI	GAGTC	7 cut(s)	30, 235, 568, 1315, 1949, 2009, 2108
ScrFI	CCNGG	7 cut(s)	735, 1267, 1583, 1682, 1991, 2061, 2190
SduI	GDGCHC	5 cut(s)	9, 31, 142, 1571, 1630
SfaNI	GCATC	6 cut(s)	946, 1212, 1414, 2293, 2310, 2356
SfcI	CTRYAG	1 cut(s)	2039
SfoI	GGCGCC	1 cut(s)	253
Sfr303I	CCGCGG	1 cut(s)	189
SgrBI	CCGCGG	1 cut(s)	189
SinI	GGWCC	3 cut(s)	21, 566, 1061
SmiMI	CAYNNNNRTG	4 cut(s)	492, 999, 1230, 1766
SmlI	CTYRAG	1 cut(s)	1201
SmoI	CTYRAG	1 cut(s)	1201
SseBI	AGGCCT	2 cut(s)	1434, 2188
SspDI	GGCGCC	1 cut(s)	251
SspMI	CTAG	5 cut(s)	119, 563, 1350, 2220, 2250
StuI	AGGCCT	2 cut(s)	1434, 2188
StyD4I	CCNGG	7 cut(s)	733, 1265, 1581, 1680, 1989, 2059, 2188
StyI	CCWWGG	3 cut(s)	1601, 1825, 2208
TaiI	ACGT	3 cut(s)	943, 1003, 1230
TaqI	TCGA	3 cut(s)	459, 716, 2361
TatI	WGTACW	1 cut(s)	834
TauI	GCSGC	3 cut(s)	176, 1510, 2091
TfiI	GAWTC	4 cut(s)	497, 713, 1286, 1714
Tru1I	TTAA	6 cut(s)	276, 773, 803, 984, 1911, 2280
Tru9I	TTAA	6 cut(s)	276, 773, 803, 984, 1911, 2280
TscAI	CASTG	8 cut(s)	31, 1099, 1466, 1509, 1773, 2048, 2124, 2181
TseFI	GTSAC	4 cut(s)	860, 1319, 1496, 2001
TseI	GCWGC	1 cut(s)	2065
Tsp45I	GTSAC	4 cut(s)	860, 1319, 1496, 2001
TspDTI	ATGAA	9 cut(s)	696, 726, 840, 915, 981, 1068, 1177, 1382, 1935
TspGWI	ACGGA	4 cut(s)	132, 180, 1704, 1722
TspRI	CASTG	8 cut(s)	31, 1099, 1466, 1509, 1773, 2048, 2124, 2181
Van91I	CCANNNNNTGG	1 cut(s)	1281
VneI	GTGCAC	1 cut(s)	1626
VpaK11BI	GGWCC	3 cut(s)	21, 566, 1061
VspI	ATTAAT	1 cut(s)	1911
XapI	RAATTY	6 cut(s)	455, 551, 874, 1751, 1798, 1982
XceI	RCATGY	4 cut(s)	413, 692, 1108, 1453
XmiI	GTMKAC	3 cut(s)	288, 1299, 2214
XmnI	GAANNNNTTC	1 cut(s)	2018
XspI	CTAG	5 cut(s)	119, 563, 1350, 2220, 2250
ZrmI	AGTACT	1 cut(s)	836
Zsp2I	ATGCAT	1 cut(s)	690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.