Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0003151

Physical Location & Seq

Genomic Coordinates Forward (+)

12462801 .. 12472648

Gene Structure 9848 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00343210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGGCCTGCACGGTACCTTCTTTTGATGATAAGTTCAACTGGGAAGGAGATTTACCTCTGAAGTATCCACAAAAAGATCTTATCATATATGAAATGCATGTGCGAGGTTTTACAAGGCATGAATCGAGCATGACTGAATTGCCTGGTACATACTGTGGTTTGGTGGAGAAGCTTGATCACTTAAAGGAACTTGGGATCAACTGTATAGAGTTAATGCCATGTCATGAGTTCAATGAACTGGAGTACTTTGGCTACAATTCTGTCTTGGGTGACTACAAGGTAAATTTTTGGGGATATTCAACTGTCAGTTACTTTTCACCAATGATAAGATATTCATCCGCTGGCACACGTAATTGTGGCCGTGATGCAATAAATGAAGTCAAGTTTCTTGTTAAAGAAGCACATAGACGTGGAATTGAGGTGATCATGGATGTTGTTTTCAATCACACAGCTGAAGGGAATGAAAATGGTCCCATTTTGTCTTTTAGAGGTGCTGACAACAGTGTCTATTACATGCTCGCCCCTAAGGGAGAGTTTTATAATTATTCAGGGTGTGGTAACACATTCAATTGCAACCATCCTGTTGTGCGACAATTCATAGTAGATTGCTTGAGATATTGGGTAACAGAGATGCACGTGGATGGGTTCCGCTTTGATCTTGCTTCTATTATGACCAGGGGTACCAGTCTCTGGGATTCAACTAATGTATACGGGAGTCTGATAGAAGGTGACTTGTTGACAACTGGAACCCCTCTCGCTAGCCCTCCATTGATTGACATGATTAGTAATGACCCAATACTTCATGGAGTGAAGCTGCTAGAAGGATTACTGATTGGGCGTTCTTTGCCATTTCCCAAACAGCTGATAGCTGAAGCTTGGGATGCAGGAGGCCTGTACCAACTTGGCATGTTTCCTCACTGGGGTATTTGGTCAGAATGGAACGGGAAGTATCGTGACACAGTGCGGCAGTTTATCAAGGGTACAGATGGCTTTTCTGGGGCTTTGGCCGAATGCCTTTGTGGGAGCCCTAATTTGTACCAGGAAGGGGGAGGGAGACCATGGAACAGTATCAATTTTATTTGTGCACACGATGGTTTTACTTTAGCTGATTTAGTAACCTATAACAACAAACATAACCTGGCAAACGGAGAAGACAACAATGACGGAGAGAATCATAATAATAGCTGGAACTGCGGACAGGAGGGAGAATTCACAAGCATTTCAGTGAAAAAACTGAGGAAGCGACAAATGCGGAATTTTTTTGTCTGCCTCATGGTTTCCCAAATTAATTACTTCCGGTGGGATAAGAAGGAAGAGTCTTCATCAGATTTTTTCAGATTTTGCTGTCTTATGACCAAATTCCGCCAGGAATGTGAGTCACTTGGTTTGAATGACTTCCCAACAGCAGAGAGACTGCAGTGGCATGGTCCTTCTCCTGGGCAGCCAGACTGGTCTAAAACAAGCCGCTTTGTGGCCTTTACTCTGGTTGACTCAGTGAAGAGAGAGATCTACATTGCCTTCAATGCCAGTCATTTACCTGTGACCATTTCACTGCCAGAGAGGCCTGGATACAGATGGGAACCCTTGGTAGACACTAGCAAGCCTTCACCCTTTGACTTCCTATCTAGTGACCTCCCACAAAGAGATATTGCAGTTAAACAGTATGCTCACTTTCTTGATGCCAATTTATACCCGATGCTAAGTTATTCTTCAATCATCCTATTGCTCGCTCCAGTCGAAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0010021 GO:0010368 GO:0015980 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0032991 GO:0043033 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1902494 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

582

Amino Acids

66.25

Weight (kDa)

5.38

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	43 - 153	2.3e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	473 - 574	1.1e-46	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003151)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39930 AT2G39930 AT2G39930
fragaria_vesca	FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440 FvH4_4g06440
malus_domestica	MD13G1236000.v1.1 MD16G1240700.v1.1 MD16G1240800.v1.1 MD16G1241000.v1.1
prunus_persica	Prupe.1G067700_v2.0.a1 Prupe.1G067700_v2.0.a1
pyrus_communis	pycom13g20870 pycom13g20880 pycom13g21910 pycom16g20170
rosa_chinensis	RchiOBHm_Chr4g0398221 RchiOBHm_Chr5g0038481
rosa_laevigata	RLG00000009377
rosa_multiflora	Rmu_sc0013706.1_g000001 Rmu_sc0020611.1_g000004 Rmu_sc0032626.1_g000001 Rmu_ssc0000049.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343210
rosa_rugosa	Rorug04G0007100 Rorug04G0007100
rosa_samantha	Rh4AG084200 Rh4BG081600 Rh4CG091500 Rh4CG091600 Rh4DG076700
rosa_wichuraiana	Rw4G007010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	540
AasI	GACNNNNNNGTC	1 cut(s)	503
Acc65I	GGTACC	2 cut(s)	13, 680
AccB1I	GGYRCC	2 cut(s)	13, 680
AccB7I	CCANNNNNTGG	1 cut(s)	690
AccI	GTMKAC	2 cut(s)	708, 1590
AciI	CCGC	7 cut(s)	339, 649, 964, 1194, 1252, 1363, 1465
AclWI	GGATC	1 cut(s)	203
AcoI	YGGCCR	2 cut(s)	358, 1005
AcsI	RAATTY	4 cut(s)	283, 1208, 1255, 1358
AcuI	CTGAAG	3 cut(s)	80, 474, 891
AcvI	CACGTG	1 cut(s)	637
AfaI	GTAC	7 cut(s)	15, 148, 245, 682, 896, 982, 1037
AfiI	CCNNNNNNNGG	5 cut(s)	690, 920, 1045, 1436, 1471
AflIII	ACRYGT	1 cut(s)	347
AgsI	TTSAA	9 cut(s)	37, 232, 300, 442, 568, 699, 1390, 1522, 1713
AjiI	CACGTC	1 cut(s)	410
AjnI	CCWGG	7 cut(s)	142, 674, 1038, 1137, 1365, 1435, 1564
AluBI	AGCT	8 cut(s)	172, 452, 814, 862, 869, 875, 1106, 1185
AluI	AGCT	8 cut(s)	172, 452, 814, 862, 869, 875, 1106, 1185
Alw21I	GWGCWC	1 cut(s)	1087
Alw26I	GTCTC	3 cut(s)	692, 1048, 1405
Alw44I	GTGCAC	1 cut(s)	1083
AlwI	GGATC	1 cut(s)	203
AlwNI	CAGNNNCTG	1 cut(s)	690
AoxI	GGCC	6 cut(s)	3, 358, 889, 1005, 1473, 1562
ApaLI	GTGCAC	1 cut(s)	1083
ApeKI	GCWGC	2 cut(s)	814, 1441
ApoI	RAATTY	4 cut(s)	283, 1208, 1255, 1358
AseI	ATTAAT	1 cut(s)	1287
Asp700I	GAANNNNTTC	1 cut(s)	1394
Asp718I	GGTACC	2 cut(s)	13, 680
AspS9I	GGNCC	2 cut(s)	470, 1427
AsuHPI	GGTGA	5 cut(s)	281, 309, 433, 740, 1599
AsuNHI	GCTAGC	1 cut(s)	758
AvaII	GGWCC	2 cut(s)	470, 1427
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	1 cut(s)	1087
BanI	GGYRCC	2 cut(s)	13, 680
BanII	GRGCYC	1 cut(s)	1028
BarI	GAAGNNNNNNTAC	2 cut(s)	36, 68
BbrPI	CACGTG	1 cut(s)	637
BbsI	GAAGAC	2 cut(s)	1158, 1311
Bbv12I	GWGCWC	1 cut(s)	1087
BbvI	GCAGC	2 cut(s)	801, 1453
BccI	CCATC	5 cut(s)	585, 635, 980, 1085, 1569
BceAI	ACGGC	1 cut(s)	345
BciT130I	CCWGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
BciVI	GTATCC	2 cut(s)	75, 1562
BclI	TGATCA	2 cut(s)	175, 423
BcoDI	GTCTC	3 cut(s)	692, 1048, 1405
BfaI	CTAG	4 cut(s)	759, 818, 1596, 1626
BfmI	CTRYAG	1 cut(s)	1415
BfuI	GTATCC	2 cut(s)	75, 1562
BglI	GCCNNNNNGGC	1 cut(s)	1561
BglII	AGATCT	2 cut(s)	76, 1506
BisI	GCNGC	4 cut(s)	815, 965, 1442, 1465
BlsI	GCNGC	4 cut(s)	816, 966, 1443, 1466
BmcAI	AGTACT	1 cut(s)	245
Bme1390I	CCNGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
Bme18I	GGWCC	2 cut(s)	470, 1427
BmgBI	CACGTC	1 cut(s)	410
BmgT120I	GGNCC	2 cut(s)	470, 1427
BmiI	GGNNCC	7 cut(s)	15, 472, 647, 682, 748, 1025, 1581
BmrFI	CCNGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
BmrI	ACTGGG	2 cut(s)	49, 928
BmsI	GCATC	6 cut(s)	355, 621, 871, 1669, 1686, 1732
BmtI	GCTAGC	1 cut(s)	762
BmuI	ACTGGG	2 cut(s)	49, 928
BpiI	GAAGAC	2 cut(s)	1158, 1311
BpmI	CTGGAG	2 cut(s)	260, 1716
BpuEI	CTTGAG	1 cut(s)	631
BsaAI	YACGTR	2 cut(s)	350, 637
BsaBI	GATNNNNATC	1 cut(s)	334
BsaI	GGTCTC	1 cut(s)	1048
BsaJI	CCNNGG	4 cut(s)	675, 1058, 1436, 1584
BsaWI	WCCGGW	1 cut(s)	1296
Bsc4I	CCNNNNNNNGG	5 cut(s)	690, 920, 1045, 1436, 1471
Bse1I	ACTGG	8 cut(s)	44, 243, 684, 748, 923, 1454, 1527, 1733
Bse21I	CCTNAGG	1 cut(s)	525
Bse3DI	GCAATG	1 cut(s)	1512
Bse8I	GATNNNNATC	1 cut(s)	334
BseBI	CCWGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
BseDI	CCNNGG	4 cut(s)	675, 1058, 1436, 1584
BseGI	GGATG	6 cut(s)	335, 436, 577, 646, 886, 1716
BseJI	GATNNNNATC	1 cut(s)	334
BseLI	CCNNNNNNNGG	5 cut(s)	690, 920, 1045, 1436, 1471
BseMI	GCAATG	1 cut(s)	1512
BseMII	CTCAG	2 cut(s)	1226, 1506
BseNI	ACTGG	8 cut(s)	44, 243, 684, 748, 923, 1454, 1527, 1733
BseSI	GKGCMC	1 cut(s)	1087
BseXI	GCAGC	2 cut(s)	801, 1453
BshFI	GGCC	6 cut(s)	5, 360, 891, 1007, 1475, 1564
BshNI	GGYRCC	2 cut(s)	13, 680
BsiHKAI	GWGCWC	1 cut(s)	1087
BsiSI	CCGG	1 cut(s)	1297
BslFI	GGGAC	1 cut(s)	456
BslI	CCNNNNNNNGG	5 cut(s)	690, 920, 1045, 1436, 1471
BsmAI	GTCTC	3 cut(s)	692, 1048, 1405
BsmFI	GGGAC	1 cut(s)	456
BsmI	GAATGC	1 cut(s)	1016
BsnI	GGCC	6 cut(s)	5, 360, 891, 1007, 1475, 1564
Bso31I	GGTCTC	1 cut(s)	1048
Bsp1286I	GDGCHC	2 cut(s)	1028, 1087
Bsp143I	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
Bsp19I	CCATGG	1 cut(s)	1058
BspACI	CCGC	7 cut(s)	339, 649, 964, 1194, 1252, 1363, 1465
BspANI	GGCC	6 cut(s)	5, 360, 891, 1007, 1475, 1564
BspCNI	CTCAG	2 cut(s)	1227, 1505
BspHI	TCATGA	1 cut(s)	223
BspLI	GGNNCC	7 cut(s)	15, 472, 647, 682, 748, 1025, 1581
BspMAI	CTGCAG	1 cut(s)	1419
BspOI	GCTAGC	1 cut(s)	762
BspPI	GGATC	1 cut(s)	203
BspT107I	GGYRCC	2 cut(s)	13, 680
BspTNI	GGTCTC	1 cut(s)	1048
BsrDI	GCAATG	1 cut(s)	1512
BsrI	ACTGG	8 cut(s)	44, 243, 684, 748, 923, 1454, 1527, 1733
BssECI	CCNNGG	4 cut(s)	675, 1058, 1436, 1584
BssMI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
BssNAI	GTATAC	1 cut(s)	709
BssT1I	CCWWGG	2 cut(s)	1058, 1584
Bst1107I	GTATAC	1 cut(s)	709
Bst2UI	CCWGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
Bst4CI	ACNGT	8 cut(s)	13, 155, 203, 304, 503, 961, 1067, 1662
Bst6I	CTCTTC	2 cut(s)	1308, 1493
BstBAI	YACGTR	2 cut(s)	350, 637
BstC8I	GCNNGC	6 cut(s)	7, 343, 519, 760, 1601, 1728
BstDEI	CTNAG	5 cut(s)	525, 1235, 1492, 1700, 1746
BstDSI	CCRYGG	1 cut(s)	1058
BstF5I	GGATG	6 cut(s)	335, 436, 577, 646, 886, 1716
BstKTI	GATC	6 cut(s)	79, 178, 198, 426, 658, 1509
BstMAI	GTCTC	3 cut(s)	692, 1048, 1405
BstMBI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
BstMWI	GCNNNNNNNGC	6 cut(s)	844, 881, 1191, 1249, 1523, 1561
BstNI	CCWGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
BstNSI	RCATGY	3 cut(s)	101, 517, 910
BstSCI	CCNGG	7 cut(s)	142, 674, 1038, 1137, 1365, 1435, 1564
BstSFI	CTRYAG	1 cut(s)	1415
BstSLI	GKGCMC	1 cut(s)	1087
BstV1I	GCAGC	2 cut(s)	801, 1453
BstV2I	GAAGAC	2 cut(s)	1158, 1311
BstX2I	RGATCY	2 cut(s)	76, 1506
BstYI	RGATCY	2 cut(s)	76, 1506
BstZ17I	GTATAC	1 cut(s)	709
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	2 cut(s)	75, 1562
BsuRI	GGCC	6 cut(s)	5, 360, 891, 1007, 1475, 1564
BtgI	CCRYGG	1 cut(s)	1058
BtrI	CACGTC	1 cut(s)	410
BtsCI	GGATG	6 cut(s)	335, 436, 577, 646, 886, 1716
BtsI	GCAGTG	2 cut(s)	1424, 1550
BtsIMutI	CAGTG	7 cut(s)	508, 916, 966, 1230, 1424, 1500, 1550
Cac8I	GCNNGC	6 cut(s)	7, 343, 519, 760, 1601, 1728
CaiI	CAGNNNCTG	1 cut(s)	690
CciI	TCATGA	1 cut(s)	223
Cfr13I	GGNCC	2 cut(s)	470, 1427
Csp6I	GTAC	7 cut(s)	14, 147, 244, 681, 895, 981, 1036
CviQI	GTAC	7 cut(s)	14, 147, 244, 681, 895, 981, 1036
DdeI	CTNAG	5 cut(s)	525, 1235, 1492, 1700, 1746
DpnI	GATC	6 cut(s)	78, 177, 197, 425, 657, 1508
DpnII	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
DrdI	GACNNNNNNGTC	1 cut(s)	503
DseDI	GACNNNNNNGTC	1 cut(s)	503
EaeI	YGGCCR	2 cut(s)	358, 1005
Eam1104I	CTCTTC	2 cut(s)	1308, 1493
EarI	CTCTTC	2 cut(s)	1308, 1493
EciI	GGCGGA	1 cut(s)	1352
Eco130I	CCWWGG	2 cut(s)	1058, 1584
Eco147I	AGGCCT	2 cut(s)	891, 1564
Eco24I	GRGCYC	1 cut(s)	1028
Eco31I	GGTCTC	1 cut(s)	1048
Eco47I	GGWCC	2 cut(s)	470, 1427
Eco57I	CTGAAG	3 cut(s)	80, 474, 891
Eco72I	CACGTG	1 cut(s)	637
Eco81I	CCTNAGG	1 cut(s)	525
EcoRI	GAATTC	1 cut(s)	1208
EcoRII	CCWGG	7 cut(s)	142, 674, 1038, 1137, 1365, 1435, 1564
EcoT14I	CCWWGG	2 cut(s)	1058, 1584
EcoT22I	ATGCAT	1 cut(s)	99
EcoT38I	GRGCYC	1 cut(s)	1028
ErhI	CCWWGG	2 cut(s)	1058, 1584
FalI	AAGNNNNNCTT	2 cut(s)	1693, 1725
FaqI	GGGAC	1 cut(s)	456
FbaI	TGATCA	2 cut(s)	175, 423
FblI	GTMKAC	2 cut(s)	708, 1590
Fnu4HI	GCNGC	4 cut(s)	815, 965, 1442, 1465
FokI	GGATG	6 cut(s)	322, 443, 564, 653, 893, 1703
FriOI	GRGCYC	1 cut(s)	1028
Fsp4HI	GCNGC	4 cut(s)	815, 965, 1442, 1465
FspBI	CTAG	4 cut(s)	759, 818, 1596, 1626
GluI	GCNGC	4 cut(s)	815, 965, 1442, 1465
GsuI	CTGGAG	2 cut(s)	260, 1716
HaeIII	GGCC	6 cut(s)	5, 360, 891, 1007, 1475, 1564
HapII	CCGG	1 cut(s)	1297
HincII	GTYRAC	2 cut(s)	738, 1489
HindII	GTYRAC	2 cut(s)	738, 1489
HindIII	AAGCTT	2 cut(s)	170, 873
HinfI	GANTC	7 cut(s)	122, 695, 715, 1171, 1316, 1376, 1490
HpaII	CCGG	1 cut(s)	1297
HphI	GGTGA	5 cut(s)	281, 309, 433, 740, 1599
Hpy166II	GTNNAC	5 cut(s)	709, 738, 1085, 1489, 1591
Hpy188I	TCNGA	5 cut(s)	60, 720, 934, 1327, 1337
Hpy188III	TCNNGA	3 cut(s)	224, 953, 1676
Hpy8I	GTNNAC	5 cut(s)	709, 738, 1085, 1489, 1591
HpyCH4III	ACNGT	8 cut(s)	13, 155, 203, 304, 503, 961, 1067, 1662
HpyCH4IV	ACGT	3 cut(s)	349, 409, 636
HpyCH4V	TGCA	9 cut(s)	9, 97, 368, 573, 634, 884, 1085, 1417, 1652
HpyF10VI	GCNNNNNNNGC	6 cut(s)	844, 881, 1191, 1249, 1523, 1561
HpyF3I	CTNAG	5 cut(s)	525, 1235, 1492, 1700, 1746
HpySE526I	ACGT	3 cut(s)	349, 409, 636
KpnI	GGTACC	2 cut(s)	17, 684
Ksp22I	TGATCA	2 cut(s)	175, 423
Kzo9I	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
LmnI	GCTCC	2 cut(s)	1023, 1735
Lsp1109I	GCAGC	2 cut(s)	801, 1453
LweI	GCATC	6 cut(s)	355, 621, 871, 1669, 1686, 1732
MaeI	CTAG	4 cut(s)	759, 818, 1596, 1626
MaeII	ACGT	3 cut(s)	349, 409, 636
MalI	GATC	6 cut(s)	78, 177, 197, 425, 657, 1508
MboI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
MboII	GAAGA	5 cut(s)	1163, 1311, 1325, 1510, 1701
MfeI	CAATTG	1 cut(s)	568
MflI	RGATCY	2 cut(s)	76, 1506
MhlI	GDGCHC	2 cut(s)	1028, 1087
MlyI	GAGTC	4 cut(s)	724, 1325, 1385, 1484
Mph1103I	ATGCAT	1 cut(s)	99
MroXI	GAANNNNTTC	1 cut(s)	1394
MseI	TTAA	5 cut(s)	182, 212, 393, 1287, 1656
MslI	CAYNNNNRTG	3 cut(s)	408, 639, 1223
MspA1I	CMGCKG	3 cut(s)	341, 452, 862
MspI	CCGG	1 cut(s)	1297
MspR9I	CCNGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
MunI	CAATTG	1 cut(s)	568
Mva1269I	GAATGC	1 cut(s)	1016
MvaI	CCWGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
MwoI	GCNNNNNNNGC	6 cut(s)	844, 881, 1191, 1249, 1523, 1561
NcoI	CCATGG	1 cut(s)	1058
NdeII	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
NheI	GCTAGC	1 cut(s)	758
NlaIV	GGNNCC	7 cut(s)	15, 472, 647, 682, 748, 1025, 1581
NmuCI	GTSAC	6 cut(s)	269, 728, 953, 1377, 1540, 1628
NsiI	ATGCAT	1 cut(s)	99
NspI	RCATGY	3 cut(s)	101, 517, 910
PagI	TCATGA	1 cut(s)	223
PceI	AGGCCT	2 cut(s)	891, 1564
PcsI	WCGNNNNNNNCGW	1 cut(s)	1734
PctI	GAATGC	1 cut(s)	1016
PdmI	GAANNNNTTC	1 cut(s)	1394
PfeI	GAWTC	3 cut(s)	122, 695, 1171
PflMI	CCANNNNNTGG	1 cut(s)	690
PkrI	GCNGC	4 cut(s)	816, 966, 1443, 1466
PleI	GAGTC	4 cut(s)	723, 1324, 1384, 1484
PmaCI	CACGTG	1 cut(s)	637
PmlI	CACGTG	1 cut(s)	637
PpsI	GAGTC	4 cut(s)	723, 1324, 1384, 1484
Ppu21I	YACGTR	2 cut(s)	350, 637
PshBI	ATTAAT	1 cut(s)	1287
PsiI	TTATAA	1 cut(s)	540
Psp6I	CCWGG	7 cut(s)	142, 674, 1038, 1137, 1365, 1435, 1564
PspCI	CACGTG	1 cut(s)	637
PspGI	CCWGG	7 cut(s)	142, 674, 1038, 1137, 1365, 1435, 1564
PspN4I	GGNNCC	7 cut(s)	15, 472, 647, 682, 748, 1025, 1581
PspPI	GGNCC	2 cut(s)	470, 1427
PstI	CTGCAG	1 cut(s)	1419
PstNI	CAGNNNCTG	1 cut(s)	690
PsuI	RGATCY	2 cut(s)	76, 1506
PvuII	CAGCTG	2 cut(s)	452, 862
RsaI	GTAC	7 cut(s)	15, 148, 245, 682, 896, 982, 1037
RsaNI	GTAC	7 cut(s)	14, 147, 244, 681, 895, 981, 1036
RseI	CAYNNNNRTG	3 cut(s)	408, 639, 1223
SaqAI	TTAA	5 cut(s)	182, 212, 393, 1287, 1656
SatI	GCNGC	4 cut(s)	815, 965, 1442, 1465
Sau3AI	GATC	6 cut(s)	76, 175, 195, 423, 655, 1506
Sau96I	GGNCC	2 cut(s)	470, 1427
ScaI	AGTACT	1 cut(s)	245
SchI	GAGTC	4 cut(s)	724, 1325, 1385, 1484
ScrFI	CCNGG	7 cut(s)	144, 676, 1040, 1139, 1367, 1437, 1566
SduI	GDGCHC	2 cut(s)	1028, 1087
SfaNI	GCATC	6 cut(s)	355, 621, 871, 1669, 1686, 1732
SfcI	CTRYAG	1 cut(s)	1415
SinI	GGWCC	2 cut(s)	470, 1427
SmiMI	CAYNNNNRTG	3 cut(s)	408, 639, 1223
SmlI	CTYRAG	1 cut(s)	610
SmoI	CTYRAG	1 cut(s)	610
SseBI	AGGCCT	2 cut(s)	891, 1564
SsiI	CCGC	7 cut(s)	339, 649, 964, 1194, 1252, 1363, 1465
SspMI	CTAG	4 cut(s)	759, 818, 1596, 1626
StuI	AGGCCT	2 cut(s)	891, 1564
StyD4I	CCNGG	7 cut(s)	142, 674, 1038, 1137, 1365, 1435, 1564
StyI	CCWWGG	2 cut(s)	1058, 1584
TaaI	ACNGT	8 cut(s)	13, 155, 203, 304, 503, 961, 1067, 1662
TaiI	ACGT	3 cut(s)	352, 412, 639
TaqI	TCGA	2 cut(s)	125, 1737
TatI	WGTACW	1 cut(s)	243
TauI	GCSGC	2 cut(s)	967, 1467
TfiI	GAWTC	3 cut(s)	122, 695, 1171
Tru1I	TTAA	5 cut(s)	182, 212, 393, 1287, 1656
Tru9I	TTAA	5 cut(s)	182, 212, 393, 1287, 1656
TscAI	CASTG	7 cut(s)	508, 923, 966, 1230, 1424, 1500, 1557
TseFI	GTSAC	6 cut(s)	269, 728, 953, 1377, 1540, 1628
TseI	GCWGC	2 cut(s)	814, 1441
Tsp45I	GTSAC	6 cut(s)	269, 728, 953, 1377, 1540, 1628
TspDTI	ATGAA	9 cut(s)	105, 135, 249, 324, 390, 477, 586, 791, 1311
TspGWI	ACGGA	2 cut(s)	1161, 1179
TspRI	CASTG	7 cut(s)	508, 923, 966, 1230, 1424, 1500, 1557
Van91I	CCANNNNNTGG	1 cut(s)	690
VneI	GTGCAC	1 cut(s)	1083
VpaK11BI	GGWCC	2 cut(s)	470, 1427
VspI	ATTAAT	1 cut(s)	1287
XapI	RAATTY	4 cut(s)	283, 1208, 1255, 1358
XceI	RCATGY	3 cut(s)	101, 517, 910
XmiI	GTMKAC	2 cut(s)	708, 1590
XmnI	GAANNNNTTC	1 cut(s)	1394
XspI	CTAG	4 cut(s)	759, 818, 1596, 1626
ZrmI	AGTACT	1 cut(s)	245
Zsp2I	ATGCAT	1 cut(s)	99

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.