AT2G43180

Phosphoenolpyruvate phosphomutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004495
Physical Location & Seq
Reverse (-)
17953497 .. 17955829
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43180.3

Sequence Viewer

Length: 1440 bp
ATGGCGGCACTTAGCTTTTGCAGCTCTTCTCCTCCTCGAATTCTTCATAAACAATCCAAATCGAGTTTTTTCCCTTCTTCTGCCTCTTCTTCTTTCACACAAACCGTCGGAGAATTATTTGCGAACCCCTTCTCTGTCTCAAGACGATTTCGTGGCGCCGTTAACTCCACCGTAGTTTCCGATGAATCGGGAAACGCGAAACTTCCATCTTCTCCGGCGAAGAAGCTAAGGAATATTATGCAATCCCCTGGTGTTCTTCAGGGTCCATGTTGCTTCGATGCTCTTAGTGCCAAACTCATCGAACGAGCTGGGTTTCCTTACTGTATCACCTCTGGGTTCTCGATTTCAGCGTCTCGGCTTGGTTTACCGGATAAGGGTCTAATTTCTTATGGAGAAATGGTTGATCAAGGTCAACAAATTACTCAATCTGTATCAATTCCGGTGATTGGAGACGGTGGCAATGGATACGGTAATGCCATGAATGTTAAGAGAACCGTCAAAGGATATATTAAAGCTGGCTTTGCAGGAATTATCATCAATGATGAAGTTTGTTGTGAAAATACTAAGAGTGAGAGAAGAGTGGTTTCTAGAGAGGAAGCAGTGATGCGTGTTAAAGCTGCGGTTGATGCACGTAGAGAGTGTGATTCGGACATTGTAATTGTAGCTCAAACTGATTCCCGTGAAGCGATATCTCTGGAGGAATCACTCATTAGGGCAAGAGCTTTTACAGATGCTGGAGCTGATGTTCTCTCCGTTGATTCTCTTGTTTCTAGAGAAGAGATGAAAGCATTCTGCAATGTCTATCCACTAGTTCCTAAATTGGCTAATATGCTTGAAAGCGGAGGAAAAATTCCAATTCTAAACCCGCTGGAGCTAGAAGAGATTGGATATAAGCTAGTGGCGTATCCAATTTCATTGATTGGAGTATCTATTCAAGCAATGCAGGACGCTCTATTGGCAATCAAGGGTGGGCGAATTCCTCCACCAGGAAGCATGGCGTCTTTAGAAGAAATTGGAGAGATTCTTGGTTTTGACACTTACGAGGAAGAAGAGAAGCGGTACGCTACCTCGTCATCAGATAGAGAAGTGAGCAGCAGTAGCGTCTATCGTAATCAACGGGTGGCTAAAGATGATCCAGAACAGAGAGAGGACCTGATTGTTGAAGTCATAACACCTGAGGTTTATAATGAACCAAGAAACCCCTTTTCGCGGATCTGGTCACGATCTTTGAGGATCAAAATCATCGGACGCGATGGGTTTGAGAAACTCGATGTCAGAATTCCGGCCGGATTCTTGGAAGGTGTCACAAACATTGTTCCAGCTTTAGGAGGCGTGAACTTGAAGCAATTGATGGACGATGCAGCAGATGAGGTCGGAGGGAAAATTTTGTTAGATTTTAAAGACACTGCAGGTGACAGAATCCAAGTCTTTCTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_mutase

Protein Analysis

479

Amino Acids

51.99

Weight (kDa)

5.22

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP_mutase PF13714 77 - 321 4.9e-41 Phosphoenolpyruvate phosphomutase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
AarI CACCTGC 1 cut(s) 1400
Acc36I ACCTGC 1 cut(s) 1400
AccB1I GGYRCC 1 cut(s) 155
AccII CGCG 3 cut(s) 197, 1210, 1251
AciI CCGC 6 cut(s) 5, 620, 840, 866, 1057, 1210
AclWI GGATC 3 cut(s) 1127, 1220, 1241
AcoI YGGCCR 1 cut(s) 1284
AcsI RAATTY 5 cut(s) 39, 849, 975, 1278, 1383
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 2 cut(s) 156, 998
AfaI GTAC 1 cut(s) 1061
AfiI CCNNNNNNNGG 5 cut(s) 374, 446, 986, 1209, 1325
AgsI TTSAA 4 cut(s) 836, 935, 1163, 1342
AhlI ACTAGT 1 cut(s) 808
AjnI CCWGG 2 cut(s) 247, 985
AloI GAACNNNNNNTCC 2 cut(s) 729, 761
Alw26I GTCTC 3 cut(s) 142, 357, 444
AlwI GGATC 3 cut(s) 1127, 1220, 1241
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 1 cut(s) 1284
ApeKI GCWGC 4 cut(s) 21, 617, 1092, 1361
ApoI RAATTY 5 cut(s) 39, 849, 975, 1278, 1383
Asp700I GAANNNNTTC 2 cut(s) 128, 788
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 263, 1150
AsuHPI GGTGA 3 cut(s) 319, 454, 1424
AvaII GGWCC 2 cut(s) 263, 1150
AxyI CCTNAGG 1 cut(s) 1176
BanI GGYRCC 1 cut(s) 155
BbvI GCAGC 4 cut(s) 33, 604, 1104, 1373
BccI CCATC 3 cut(s) 214, 1247, 1345
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 249, 987
BciVI GTATCC 2 cut(s) 458, 915
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 3 cut(s) 142, 357, 444
BcuI ACTAGT 1 cut(s) 808
BfaI CTAG 6 cut(s) 588, 771, 809, 875, 896, 1433
BfmI CTRYAG 1 cut(s) 1407
BfoI RGCGCY 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 1400
BfuI GTATCC 2 cut(s) 458, 915
BisI GCNGC 5 cut(s) 6, 22, 618, 1093, 1362
BlsI GCNGC 5 cut(s) 7, 23, 619, 1094, 1363
Bme1390I CCNGG 2 cut(s) 249, 987
Bme18I GGWCC 2 cut(s) 263, 1150
BmgT120I GGNCC 2 cut(s) 263, 1150
BmiI GGNNCC 2 cut(s) 157, 264
BmrFI CCNGG 2 cut(s) 249, 987
BmsI GCATC 5 cut(s) 268, 594, 616, 721, 1348
BpmI CTGGAG 3 cut(s) 716, 756, 890
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 632
BsaBI GATNNNNATC 1 cut(s) 1238
BsaHI GRCGYC 2 cut(s) 156, 998
BsaJI CCNNGG 1 cut(s) 247
BsaWI WCCGGW 2 cut(s) 367, 439
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 729, 759
Bsc4I CCNNNNNNNGG 5 cut(s) 374, 446, 986, 1209, 1325
Bse21I CCTNAGG 1 cut(s) 1176
Bse3DI GCAATG 3 cut(s) 466, 802, 945
Bse8I GATNNNNATC 1 cut(s) 1238
BseBI CCWGG 2 cut(s) 249, 987
BseDI CCNNGG 1 cut(s) 247
BseJI GATNNNNATC 1 cut(s) 1238
BseLI CCNNNNNNNGG 5 cut(s) 374, 446, 986, 1209, 1325
BseMI GCAATG 3 cut(s) 466, 802, 945
BseMII CTCAG 1 cut(s) 1167
BseRI GAGGAG 2 cut(s) 21, 24
BseX3I CGGCCG 1 cut(s) 1284
BseXI GCAGC 4 cut(s) 33, 604, 1104, 1373
BseYI CCCAGC 1 cut(s) 308
Bsh1236I CGCG 3 cut(s) 197, 1210, 1251
Bsh1285I CGRYCG 1 cut(s) 1287
BshFI GGCC 1 cut(s) 1286
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 1287
BsiSI CCGG 5 cut(s) 215, 368, 440, 1283, 1287
BslI CCNNNNNNNGG 5 cut(s) 374, 446, 986, 1209, 1325
BsmAI GTCTC 3 cut(s) 142, 357, 444
BsmBI CGTCTC 2 cut(s) 357, 444
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 1 cut(s) 1286
Bsp143I GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
BspACI CCGC 6 cut(s) 5, 620, 840, 866, 1057, 1210
BspANI GGCC 1 cut(s) 1286
BspCNI CTCAG 1 cut(s) 1168
BspFNI CGCG 3 cut(s) 197, 1210, 1251
BspLI GGNNCC 2 cut(s) 157, 264
BspMAI CTGCAG 1 cut(s) 1411
BspMI ACCTGC 1 cut(s) 1400
BspPI GGATC 3 cut(s) 1127, 1220, 1241
BspQI GCTCTTC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 3 cut(s) 466, 802, 945
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
BssNI GRCGYC 2 cut(s) 156, 998
Bst2UI CCWGG 2 cut(s) 249, 987
Bst4CI ACNGT 6 cut(s) 106, 172, 323, 455, 470, 496
Bst6I CTCTTC 6 cut(s) 31, 91, 571, 771, 873, 1044
BstACI GRCGYC 2 cut(s) 156, 998
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 1 cut(s) 517
BstDEI CTNAG 5 cut(s) 11, 227, 284, 564, 1176
BstENI CCTNNNNNAGG 1 cut(s) 984
BstFNI CGCG 3 cut(s) 197, 1210, 1251
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 5 cut(s) 406, 1135, 1215, 1226, 1236
BstMAI GTCTC 3 cut(s) 142, 357, 444
BstMBI GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
BstMCI CGRYCG 1 cut(s) 1287
BstMWI GCNNNNNNNGC 6 cut(s) 21, 287, 521, 626, 956, 1098
BstNI CCWGG 2 cut(s) 249, 987
BstSCI CCNGG 2 cut(s) 247, 985
BstSFI CTRYAG 1 cut(s) 1407
BstUI CGCG 3 cut(s) 197, 1210, 1251
BstV1I GCAGC 4 cut(s) 33, 604, 1104, 1373
BstX2I RGATCY 1 cut(s) 1212
BstYI RGATCY 1 cut(s) 1212
BstZI CGGCCG 1 cut(s) 1284
Bsu36I CCTNAGG 1 cut(s) 1176
BsuI GTATCC 2 cut(s) 458, 915
BsuRI GGCC 1 cut(s) 1286
BtgZI GCGATG 1 cut(s) 1266
BtsI GCAGTG 2 cut(s) 606, 1404
BtsIMutI CAGTG 2 cut(s) 606, 1404
BveI ACCTGC 1 cut(s) 1400
Cac8I GCNNGC 1 cut(s) 517
CaiI CAGNNNCTG 1 cut(s) 734
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 263, 1150
CseI GACGC 5 cut(s) 339, 956, 987, 1090, 1257
Csp6I GTAC 1 cut(s) 1060
CviAII CATG 3 cut(s) 267, 478, 994
CviQI GTAC 1 cut(s) 1060
DdeI CTNAG 5 cut(s) 11, 227, 284, 564, 1176
DinI GGCGCC 1 cut(s) 157
DpnI GATC 5 cut(s) 405, 1134, 1214, 1225, 1235
DpnII GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
DraI TTTAAA 1 cut(s) 1399
EaeI YGGCCR 1 cut(s) 1284
EagI CGGCCG 1 cut(s) 1284
Eam1104I CTCTTC 6 cut(s) 31, 91, 571, 771, 873, 1044
EarI CTCTTC 6 cut(s) 31, 91, 571, 771, 873, 1044
EclXI CGGCCG 1 cut(s) 1284
Eco32I GATATC 1 cut(s) 690
Eco47I GGWCC 2 cut(s) 263, 1150
Eco52I CGGCCG 1 cut(s) 1284
Eco57I CTGAAG 1 cut(s) 242
Eco81I CCTNAGG 1 cut(s) 1176
EcoNI CCTNNNNNAGG 1 cut(s) 984
EcoO109I RGGNCCY 1 cut(s) 1150
EcoRI GAATTC 3 cut(s) 39, 975, 1278
EcoRII CCWGG 2 cut(s) 247, 985
EcoRV GATATC 1 cut(s) 690
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
Esp3I CGTCTC 2 cut(s) 357, 444
FaeI CATG 3 cut(s) 270, 481, 997
FatI CATG 3 cut(s) 266, 477, 993
FauI CCCGC 1 cut(s) 873
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 5 cut(s) 6, 22, 618, 1093, 1362
Fsp4HI GCNGC 5 cut(s) 6, 22, 618, 1093, 1362
FspBI CTAG 6 cut(s) 588, 771, 809, 875, 896, 1433
GlaI GCGC 1 cut(s) 157
GluI GCNGC 5 cut(s) 6, 22, 618, 1093, 1362
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 3 cut(s) 716, 756, 890
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 1 cut(s) 1286
HapII CCGG 5 cut(s) 215, 368, 440, 1283, 1287
HgaI GACGC 5 cut(s) 339, 956, 987, 1090, 1257
HhaI GCGC 1 cut(s) 158
Hin1I GRCGYC 2 cut(s) 156, 998
Hin1II CATG 3 cut(s) 270, 481, 997
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 2 cut(s) 163, 413
HindII GTYRAC 2 cut(s) 163, 413
HinfI GANTC 8 cut(s) 185, 644, 674, 701, 758, 1021, 1290, 1419
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 5 cut(s) 215, 368, 440, 1283, 1287
HphI GGTGA 3 cut(s) 319, 454, 1424
Hpy166II GTNNAC 4 cut(s) 163, 365, 413, 1336
Hpy188I TCNGA 7 cut(s) 110, 181, 649, 1078, 1247, 1277, 1376
Hpy188III TCNNGA 9 cut(s) 141, 189, 340, 588, 695, 771, 1136, 1221, 1433
Hpy8I GTNNAC 4 cut(s) 163, 365, 413, 1336
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 3 cut(s) 84, 139, 1292
HpyCH4III ACNGT 6 cut(s) 106, 172, 323, 455, 470, 496
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 8 cut(s) 21, 241, 524, 629, 795, 943, 1361, 1409
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 287, 521, 626, 956, 1098
HpyF3I CTNAG 5 cut(s) 11, 227, 284, 564, 1176
HpySE526I ACGT 1 cut(s) 631
Hsp92I GRCGYC 2 cut(s) 156, 998
Hsp92II CATG 3 cut(s) 270, 481, 997
HspAI GCGC 1 cut(s) 156
KasI GGCGCC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 403
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
LguI GCTCTTC 1 cut(s) 31
LmnI GCTCC 2 cut(s) 737, 871
Lsp1109I GCAGC 4 cut(s) 33, 604, 1104, 1373
LweI GCATC 5 cut(s) 268, 594, 616, 721, 1348
MaeI CTAG 6 cut(s) 588, 771, 809, 875, 896, 1433
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 3 cut(s) 1218, 1303, 1412
MalI GATC 5 cut(s) 405, 1134, 1214, 1225, 1235
MboI GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
MfeI CAATTG 1 cut(s) 1346
MflI RGATCY 1 cut(s) 1212
Mly113I GGCGCC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 88, 1354
MroXI GAANNNNTTC 2 cut(s) 128, 788
MseI TTAA 5 cut(s) 162, 486, 510, 612, 1398
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 5 cut(s) 215, 368, 440, 1283, 1287
MspR9I CCNGG 2 cut(s) 249, 987
MunI CAATTG 1 cut(s) 1346
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 2 cut(s) 249, 987
MvnI CGCG 3 cut(s) 197, 1210, 1251
MwoI GCNNNNNNNGC 6 cut(s) 21, 287, 521, 626, 956, 1098
NarI GGCGCC 1 cut(s) 156
NdeII GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
NlaIII CATG 3 cut(s) 270, 481, 997
NlaIV GGNNCC 2 cut(s) 157, 264
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 1218, 1303, 1412
PaqCI CACCTGC 1 cut(s) 1400
PciSI GCTCTTC 1 cut(s) 31
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 347
PctI GAATGC 1 cut(s) 788
PdmI GAANNNNTTC 2 cut(s) 128, 788
PfeI GAWTC 8 cut(s) 185, 644, 674, 701, 758, 1021, 1290, 1419
PkrI GCNGC 5 cut(s) 7, 23, 619, 1094, 1363
PluTI GGCGCC 1 cut(s) 159
Ppu21I YACGTR 1 cut(s) 632
PpuMI RGGWCCY 1 cut(s) 1150
PsiI TTATAA 1 cut(s) 1185
Psp5II RGGWCCY 1 cut(s) 1150
Psp6I CCWGG 2 cut(s) 247, 985
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 2 cut(s) 247, 985
PspN4I GGNNCC 2 cut(s) 157, 264
PspPI GGNCC 2 cut(s) 263, 1150
PspPPI RGGWCCY 1 cut(s) 1150
PstI CTGCAG 1 cut(s) 1411
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 1 cut(s) 1212
RsaI GTAC 1 cut(s) 1061
RsaNI GTAC 1 cut(s) 1060
SapI GCTCTTC 1 cut(s) 31
SaqAI TTAA 5 cut(s) 162, 486, 510, 612, 1398
SatI GCNGC 5 cut(s) 6, 22, 618, 1093, 1362
Sau3AI GATC 5 cut(s) 403, 1132, 1212, 1223, 1233
Sau96I GGNCC 2 cut(s) 263, 1150
ScrFI CCNGG 2 cut(s) 249, 987
SfaNI GCATC 5 cut(s) 268, 594, 616, 721, 1348
SfcI CTRYAG 1 cut(s) 1407
SfoI GGCGCC 1 cut(s) 157
SinI GGWCC 2 cut(s) 263, 1150
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SpeI ACTAGT 1 cut(s) 808
SsiI CCGC 6 cut(s) 5, 620, 840, 866, 1057, 1210
SspDI GGCGCC 1 cut(s) 155
SspI AATATT 1 cut(s) 235
SspMI CTAG 6 cut(s) 588, 771, 809, 875, 896, 1433
StyD4I CCNGG 2 cut(s) 247, 985
TaaI ACNGT 6 cut(s) 106, 172, 323, 455, 470, 496
TaiI ACGT 1 cut(s) 634
TaqI TCGA 6 cut(s) 37, 62, 276, 300, 341, 1269
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 8 cut(s) 185, 644, 674, 701, 758, 1021, 1290, 1419
Tru1I TTAA 5 cut(s) 162, 486, 510, 612, 1398
Tru9I TTAA 5 cut(s) 162, 486, 510, 612, 1398
TscAI CASTG 2 cut(s) 606, 1411
TseFI GTSAC 3 cut(s) 1218, 1303, 1412
TseI GCWGC 4 cut(s) 21, 617, 1092, 1361
Tsp45I GTSAC 3 cut(s) 1218, 1303, 1412
TspDTI ATGAA 7 cut(s) 35, 198, 494, 558, 797, 903, 1203
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 2 cut(s) 606, 1411
VpaK11BI GGWCC 2 cut(s) 263, 1150
XagI CCTNNNNNAGG 1 cut(s) 984
XapI RAATTY 5 cut(s) 39, 849, 975, 1278, 1383
XbaI TCTAGA 3 cut(s) 587, 770, 1432
XmnI GAANNNNTTC 2 cut(s) 128, 788
XspI CTAG 6 cut(s) 588, 771, 809, 875, 896, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.