Rmu_sc0001258.1_g000006

Phosphoenolpyruvate phosphomutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001258.1
OG0004495
Physical Location & Seq
Reverse (-)
28162 .. 33270
5109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001258.1_g000006.1.cds

Sequence Viewer

Length: 1401 bp
atgagttgtgtgctgcagcagcagtatcctcaggtgggtagcagagactgcagctcctgtttcaatgtcagcagctcgccttcaaaccttacattcgggtcacgtcttcatcaacgatcactaaaactgccccacaagtcaaatgcgtctccttttcgtaccacaaggatcctcgcaacttactcaggagactcagcgccgccgcccgagtctccggccaaagcgcttcgccggattctggagttgccggggttcaccaaggccctgcttgctttgatggtctcagcgccaagcttgtcgaaagagctggcttccagtatggcttcaccagtggggcaacaaattacccaagctgtgtcaattcctgtcattggggatggcgataatggttacgggaatgcaatgaatgtcaagagaactgtcaagggatatataagagctcgctttgctgggattctgcttgaagatcaggtatctccaaaagcctgcggtcatacacaaggcaggaaagtggtgtcaagggaggaagctgtgatgcggataaaagcagcagttgatgctcggaaagagagtggctctgacattgtaattgtagcacgatctgattctcgtcaagcagtgtctttggaagaagcactttggaggtcaagggcattcgctgatgctggagcagatgttcttttcattgatgcactagcctcaaaagaagaaatgaaggctttctgtggagtatatcccctagttccaaaaatggcgaatatgcttgaaggagggggaaaaacaccaatactcaatcctcttgaacttgaggaagttggatacaaacttgtggcttatccactttccttgattggggtatctattcaagcaatgcaggacgcactagctgctataagaggaggtcgtatccctccacctggaagcatgccatcttttgaagaggttaaggaggtcctaggtttcaacagttattatgaagaagaaaagcggtattatagtaacagcagtcagctgtcttctgaaagattggccagtagtgtatacagtcttcaagggacatcaaaaaatgcagaaccaaaatatcagagtcctcaagatcccattgttgaagttataacacctgaagtgtacaataactatggtgctgatggtttcaaggattcattttccgggatctggtctcggaaattgagaatcaaaataactggaagagatggatttgagaaacttgatgttagaattcctgctggattcttggacggtatcaccaatatagttccagctcttgggggtgtaaacatcaaagaattgttgaatgaggcagctgatgaaatgggagggaagcagctgttagattttaatgacacaatgggtgatagaattcaagtttttctagagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_mutase

Protein Analysis

466

Amino Acids

50.54

Weight (kDa)

7.56

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1115
AccB7I CCANNNNNTGG 1 cut(s) 1286
AccI GTMKAC 1 cut(s) 1041
AciI CCGC 5 cut(s) 200, 203, 489, 538, 988
AclWI GGATC 4 cut(s) 163, 176, 1091, 1181
AcoI YGGCCR 2 cut(s) 216, 1029
AcsI RAATTY 2 cut(s) 1239, 1380
AcuI CTGAAG 1 cut(s) 1143
AfaI GTAC 2 cut(s) 160, 1130
AfeI AGCGCT 1 cut(s) 225
AfiI CCNNNNNNNGG 7 cut(s) 35, 238, 371, 852, 917, 1176, 1286
AjiI CACGTC 1 cut(s) 104
AjnI CCWGG 1 cut(s) 916
AloI GAACNNNNNNTCC 2 cut(s) 660, 692
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 6 cut(s) 39, 153, 183, 216, 286, 1185
AlwI GGATC 4 cut(s) 163, 176, 1091, 1181
AlwNI CAGNNNCTG 2 cut(s) 48, 57
Ama87I CYCGRG 1 cut(s) 206
Aor51HI AGCGCT 1 cut(s) 225
AoxI GGCC 3 cut(s) 216, 261, 1029
ApeKI GCWGC 9 cut(s) 13, 16, 19, 51, 72, 548, 887, 1322, 1345
ApoI RAATTY 2 cut(s) 1239, 1380
Asp700I GAANNNNTTC 1 cut(s) 719
AspA2I CCTAGG 1 cut(s) 955
AspLEI GCGC 3 cut(s) 199, 226, 289
AspS9I GGNCC 2 cut(s) 262, 952
AsuC2I CCSGG 2 cut(s) 249, 1171
AsuHPI GGTGA 4 cut(s) 247, 318, 1258, 1385
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 952
AvrII CCTAGG 1 cut(s) 955
AxyI CCTNAGG 1 cut(s) 30
BalI TGGCCA 1 cut(s) 1031
BamHI GGATCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 442
BarI GAAGNNNNNNTAC 2 cut(s) 1032, 1064
BbsI GAAGAC 3 cut(s) 98, 1008, 1040
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 8 cut(s) 28, 31, 63, 84, 560, 874, 1334, 1357
BccI CCATC 5 cut(s) 271, 371, 937, 1142, 1208
BciT130I CCWGG 1 cut(s) 918
BciVI GTATCC 3 cut(s) 36, 812, 917
BcnI CCSGG 2 cut(s) 249, 1171
BcoDI GTCTC 6 cut(s) 39, 153, 183, 216, 286, 1185
BfaI CTAG 5 cut(s) 695, 740, 884, 956, 1394
BfmI CTRYAG 2 cut(s) 14, 49
BfoI RGCGCY 3 cut(s) 200, 227, 290
BfuI GTATCC 3 cut(s) 36, 812, 917
BlnI CCTAGG 1 cut(s) 955
Bme1390I CCNGG 3 cut(s) 249, 918, 1171
Bme18I GGWCC 1 cut(s) 952
BmeT110I CYCGRG 1 cut(s) 206
BmgBI CACGTC 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 262, 952
BmiI GGNNCC 1 cut(s) 170
BmrFI CCNGG 3 cut(s) 249, 918, 1171
BmsI GCATC 4 cut(s) 525, 547, 652, 679
BpiI GAAGAC 3 cut(s) 98, 1008, 1040
BplI GAGNNNNNCTC 2 cut(s) 560, 592
BpmI CTGGAG 2 cut(s) 260, 687
BpuEI CTTGAG 2 cut(s) 827, 1077
BpuMI CCSGG 2 cut(s) 249, 1171
BsaI GGTCTC 2 cut(s) 286, 1185
BsaJI CCNNGG 3 cut(s) 248, 258, 955
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 660, 690
Bsc4I CCNNNNNNNGG 7 cut(s) 35, 238, 371, 852, 917, 1176, 1286
Bse1I ACTGG 4 cut(s) 315, 329, 1032, 1210
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 2 cut(s) 408, 876
BseBI CCWGG 1 cut(s) 918
BseDI CCNNGG 3 cut(s) 248, 258, 955
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 7 cut(s) 35, 238, 371, 852, 917, 1176, 1286
BseMI GCAATG 2 cut(s) 408, 876
BseMII CTCAG 4 cut(s) 44, 198, 207, 297
BseNI ACTGG 4 cut(s) 315, 329, 1032, 1210
BseRI GAGGAG 1 cut(s) 912
BseXI GCAGC 8 cut(s) 28, 31, 63, 84, 560, 874, 1334, 1357
BseYI CCCAGC 1 cut(s) 449
BshFI GGCC 3 cut(s) 218, 263, 1031
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 4 cut(s) 215, 232, 248, 1170
BslFI GGGAC 1 cut(s) 1069
BslI CCNNNNNNNGG 7 cut(s) 35, 238, 371, 852, 917, 1176, 1286
BsmAI GTCTC 6 cut(s) 39, 153, 183, 216, 286, 1185
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 1069
BsmI GAATGC 2 cut(s) 403, 653
BsnI GGCC 3 cut(s) 218, 263, 1031
Bso31I GGTCTC 2 cut(s) 286, 1185
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 442
Bsp1407I TGTACA 1 cut(s) 1128
Bsp143I GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
BspACI CCGC 5 cut(s) 200, 203, 489, 538, 988
BspANI GGCC 3 cut(s) 218, 263, 1031
BspCNI CTCAG 4 cut(s) 43, 197, 206, 296
BspLI GGNNCC 1 cut(s) 170
BspMAI CTGCAG 2 cut(s) 18, 53
BspPI GGATC 4 cut(s) 163, 176, 1091, 1181
BspTNI GGTCTC 2 cut(s) 286, 1185
BsrDI GCAATG 2 cut(s) 408, 876
BsrGI TGTACA 1 cut(s) 1128
BsrI ACTGG 4 cut(s) 315, 329, 1032, 1210
BssECI CCNNGG 3 cut(s) 248, 258, 955
BssMI GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
BssNAI GTATAC 1 cut(s) 1042
BssT1I CCWWGG 2 cut(s) 258, 955
Bst1107I GTATAC 1 cut(s) 1042
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 4 cut(s) 421, 968, 1046, 1262
Bst6I CTCTTC 2 cut(s) 933, 1204
BstAPI GCANNNNNTGC 3 cut(s) 48, 557, 887
BstAUI TGTACA 1 cut(s) 1128
BstC8I GCNNGC 6 cut(s) 77, 270, 309, 442, 487, 926
BstDEI CTNAG 4 cut(s) 30, 184, 193, 283
BstF5I GGATG 1 cut(s) 382
BstH2I RGCGCY 3 cut(s) 200, 227, 290
BstHHI GCGC 3 cut(s) 199, 226, 289
BstKTI GATC 6 cut(s) 119, 171, 469, 602, 1099, 1176
BstMAI GTCTC 6 cut(s) 39, 153, 183, 216, 286, 1185
BstMBI GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
BstMWI GCNNNNNNNGC 6 cut(s) 19, 48, 269, 446, 557, 887
BstNI CCWGG 1 cut(s) 918
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 3 cut(s) 247, 916, 1169
BstSFI CTRYAG 2 cut(s) 14, 49
BstV1I GCAGC 8 cut(s) 28, 31, 63, 84, 560, 874, 1334, 1357
BstV2I GAAGAC 3 cut(s) 98, 1008, 1040
BstX2I RGATCY 3 cut(s) 168, 1096, 1173
BstYI RGATCY 3 cut(s) 168, 1096, 1173
BstZ17I GTATAC 1 cut(s) 1042
Bsu36I CCTNAGG 1 cut(s) 30
BsuI GTATCC 3 cut(s) 36, 812, 917
BsuRI GGCC 3 cut(s) 218, 263, 1031
BtrI CACGTC 1 cut(s) 104
BtsCI GGATG 1 cut(s) 382
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 2 cut(s) 336, 624
Cac8I GCNNGC 6 cut(s) 77, 270, 309, 442, 487, 926
CaiI CAGNNNCTG 2 cut(s) 48, 57
CfoI GCGC 3 cut(s) 199, 226, 289
Cfr13I GGNCC 2 cut(s) 262, 952
CseI GACGC 2 cut(s) 135, 887
Csp6I GTAC 2 cut(s) 159, 1129
CspCI CAANNNNNGTGG 2 cut(s) 828, 863
CviAII CATG 1 cut(s) 925
CviQI GTAC 2 cut(s) 159, 1129
DdeI CTNAG 4 cut(s) 30, 184, 193, 283
DpnI GATC 6 cut(s) 118, 170, 468, 601, 1098, 1175
DpnII GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
EaeI YGGCCR 2 cut(s) 216, 1029
Eam1104I CTCTTC 2 cut(s) 933, 1204
EarI CTCTTC 2 cut(s) 933, 1204
Ecl136II GAGCTC 1 cut(s) 440
Eco130I CCWWGG 2 cut(s) 258, 955
Eco24I GRGCYC 1 cut(s) 442
Eco31I GGTCTC 2 cut(s) 286, 1185
Eco47I GGWCC 1 cut(s) 952
Eco47III AGCGCT 1 cut(s) 225
Eco53kI GAGCTC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 1143
Eco81I CCTNAGG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 440
EcoO109I RGGNCCY 2 cut(s) 262, 952
EcoRI GAATTC 2 cut(s) 1239, 1380
EcoRII CCWGG 1 cut(s) 916
EcoT14I CCWWGG 2 cut(s) 258, 955
EcoT38I GRGCYC 1 cut(s) 442
ErhI CCWWGG 2 cut(s) 258, 955
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 1 cut(s) 928
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 1 cut(s) 1069
FatI CATG 1 cut(s) 924
FblI GTMKAC 1 cut(s) 1041
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 442
FspBI CTAG 5 cut(s) 695, 740, 884, 956, 1394
GlaI GCGC 3 cut(s) 198, 225, 288
GsaI CCCAGC 1 cut(s) 453
GsuI CTGGAG 2 cut(s) 260, 687
HaeII RGCGCY 3 cut(s) 200, 227, 290
HaeIII GGCC 3 cut(s) 218, 263, 1031
HapII CCGG 4 cut(s) 215, 232, 248, 1170
HgaI GACGC 2 cut(s) 135, 887
HhaI GCGC 3 cut(s) 199, 226, 289
Hin1II CATG 1 cut(s) 928
Hin6I GCGC 3 cut(s) 197, 224, 287
HinP1I GCGC 3 cut(s) 197, 224, 287
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 9 cut(s) 191, 209, 235, 454, 605, 1087, 1160, 1194, 1251
HpaII CCGG 4 cut(s) 215, 232, 248, 1170
HphI GGTGA 4 cut(s) 247, 318, 1258, 1385
Hpy166II GTNNAC 4 cut(s) 255, 1042, 1129, 1297
Hpy188I TCNGA 6 cut(s) 564, 580, 604, 1021, 1086, 1185
Hpy188III TCNNGA 6 cut(s) 186, 239, 412, 800, 1094, 1394
Hpy8I GTNNAC 4 cut(s) 255, 1042, 1129, 1297
HpyAV CCTTC 3 cut(s) 90, 709, 761
HpyCH4III ACNGT 4 cut(s) 421, 968, 1046, 1262
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 6 cut(s) 16, 51, 401, 692, 874, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 48, 269, 446, 557, 887
HpyF3I CTNAG 4 cut(s) 30, 184, 193, 283
HpySE526I ACGT 1 cut(s) 103
Hsp92II CATG 1 cut(s) 928
HspAI GCGC 3 cut(s) 197, 224, 287
Kzo9I GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
LmnI GCTCC 2 cut(s) 59, 668
Lsp1109I GCAGC 8 cut(s) 28, 31, 63, 84, 560, 874, 1334, 1357
LweI GCATC 4 cut(s) 525, 547, 652, 679
MaeI CTAG 5 cut(s) 695, 740, 884, 956, 1394
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 3 cut(s) 99, 389, 998
MalI GATC 6 cut(s) 118, 170, 468, 601, 1098, 1175
MboI GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
MflI RGATCY 3 cut(s) 168, 1096, 1173
MhlI GDGCHC 1 cut(s) 442
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 7 cut(s) 342, 360, 588, 1187, 1239, 1307, 1380
MluNI TGGCCA 1 cut(s) 1031
MlyI GAGTC 3 cut(s) 185, 218, 1096
MmeI TCCRAC 1 cut(s) 796
Mox20I TGGCCA 1 cut(s) 1031
MroXI GAANNNNTTC 1 cut(s) 719
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 2 cut(s) 945, 1359
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 3 cut(s) 1012, 1325, 1348
MspI CCGG 4 cut(s) 215, 232, 248, 1170
MspR9I CCNGG 3 cut(s) 249, 918, 1171
Mva1269I GAATGC 2 cut(s) 403, 653
MvaI CCWGG 1 cut(s) 918
MwoI GCNNNNNNNGC 6 cut(s) 19, 48, 269, 446, 557, 887
NciI CCSGG 2 cut(s) 249, 1171
NdeII GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
NlaIII CATG 1 cut(s) 928
NlaIV GGNNCC 1 cut(s) 170
NmuCI GTSAC 1 cut(s) 99
NspI RCATGY 1 cut(s) 928
PaeI GCATGC 1 cut(s) 928
PctI GAATGC 2 cut(s) 403, 653
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 6 cut(s) 235, 454, 605, 1160, 1194, 1251
PflMI CCANNNNNTGG 1 cut(s) 1286
PfoI TCCNGGA 1 cut(s) 1169
PleI GAGTC 3 cut(s) 185, 217, 1095
PpsI GAGTC 3 cut(s) 185, 217, 1095
PpuMI RGGWCCY 1 cut(s) 952
PsiI TTATAA 1 cut(s) 1115
Psp124BI GAGCTC 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 952
Psp6I CCWGG 1 cut(s) 916
PspFI CCCAGC 1 cut(s) 449
PspGI CCWGG 1 cut(s) 916
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 2 cut(s) 262, 952
PspPPI RGGWCCY 1 cut(s) 952
PstI CTGCAG 2 cut(s) 18, 53
PstNI CAGNNNCTG 2 cut(s) 48, 57
PsuI RGATCY 3 cut(s) 168, 1096, 1173
PvuII CAGCTG 3 cut(s) 1012, 1325, 1348
RsaI GTAC 2 cut(s) 160, 1130
RsaNI GTAC 2 cut(s) 159, 1129
SacI GAGCTC 1 cut(s) 442
SaqAI TTAA 2 cut(s) 945, 1359
Sau3AI GATC 6 cut(s) 116, 168, 466, 599, 1096, 1173
Sau96I GGNCC 2 cut(s) 262, 952
SchI GAGTC 3 cut(s) 185, 218, 1096
ScrFI CCNGG 3 cut(s) 249, 918, 1171
SduI GDGCHC 1 cut(s) 442
SfaNI GCATC 4 cut(s) 525, 547, 652, 679
SfcI CTRYAG 2 cut(s) 14, 49
SinI GGWCC 1 cut(s) 952
SmlI CTYRAG 2 cut(s) 806, 1092
SmoI CTYRAG 2 cut(s) 806, 1092
SphI GCATGC 1 cut(s) 928
Sse9I AATT 7 cut(s) 342, 360, 588, 1187, 1239, 1307, 1380
SsiI CCGC 5 cut(s) 200, 203, 489, 538, 988
SspMI CTAG 5 cut(s) 695, 740, 884, 956, 1394
SstI GAGCTC 1 cut(s) 442
StyD4I CCNGG 3 cut(s) 247, 916, 1169
StyI CCWWGG 2 cut(s) 258, 955
TaaI ACNGT 4 cut(s) 421, 968, 1046, 1262
TaiI ACGT 1 cut(s) 106
TaqI TCGA 1 cut(s) 299
TasI AATT 7 cut(s) 342, 360, 588, 1187, 1239, 1307, 1380
TatI WGTACW 1 cut(s) 1128
TauI GCSGC 2 cut(s) 202, 205
TfiI GAWTC 6 cut(s) 235, 454, 605, 1160, 1194, 1251
Tru1I TTAA 2 cut(s) 945, 1359
Tru9I TTAA 2 cut(s) 945, 1359
TscAI CASTG 2 cut(s) 336, 624
TseFI GTSAC 1 cut(s) 99
TseI GCWGC 9 cut(s) 13, 16, 19, 51, 72, 548, 887, 1322, 1345
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 7 cut(s) 98, 419, 673, 728, 990, 1152, 1344
TspRI CASTG 2 cut(s) 336, 624
Van91I CCANNNNNTGG 1 cut(s) 1286
VpaK11BI GGWCC 1 cut(s) 952
XapI RAATTY 2 cut(s) 1239, 1380
XbaI TCTAGA 1 cut(s) 1393
XceI RCATGY 1 cut(s) 928
XmaJI CCTAGG 1 cut(s) 955
XmiI GTMKAC 1 cut(s) 1041
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 5 cut(s) 695, 740, 884, 956, 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.