Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004495

Physical Location & Seq

Genomic Coordinates Forward (+)

29200013 .. 29203882

Gene Structure 3870 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G012440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGGAAGCCCCAACAAGATCCTCGCAGCTTACTCAGCGCCGCCGCCGCCCGAGTCTCCGGCCAAAGCGCTCCGCCGGATTCTGGAGTTGCCGGGGGTTCACCAAGGCCCTGCTTGCTTTGATGGTCTCAGCGCCAAGCTTATTGAAAGAGCCGGCTTCCAGTATTGCTTCACCAGTGGATTTTCGATATCGGCTGCTACATTAGGGCTGCCAGATACAGGTTTTCTATCCTACGGAGAAATGGTGGATCAGGGGCAACAAATTACCCAAGCTGTGTCAATTCCTGTCATTGGGGATGGCGACAACGGTTACGGGAATGCAATGAACGTCAAGAGAACTGCCAAGGGATTTATTAGAGCTGGCTTTGCTGGCATTCTGCTTGAAGATCAGGTGTCTCCAAAAGCCTGCGGTCATACACGAGGCAGGAAAGTGGTGTCAAGGGAGGAAGCTGTGATGCGGATAAAAGCAGCAGTTGATGCTCGGAAAGAGAGTGGTTCTGACATTGTAATTGTAGCACGATCTGATGCTCGTCAAGCAGTGTCTTTGGAAGAAGCACTTTGGAGGTCAAGGGCATTCGCTGATGCTGGAGCAGATGTTCTTTTCATTGATGCACTAGCCTCAAAAGAAGAAATGAAGGCTTTCTGTGGAGTATATCCCCTAGTTCCAAAAATGGCGAATATGCTTGAAGGAGGGGGAAAAACGCCAATACTCAATCCTCTTGAACTTGAGGAAGTTGGATACAAAATTGTGGTTTATCCACTTTCCTTGATTGGGGTATCTATTCAAGCAATGCAGGACGCACTAGCTGCTATCAGAGGAGGTCGTATCCCTCCACCTGGAAGCATGCCATCTTTTGAAGAGCTTAAGGAGGTCCTAGGTTTCAACAGTTATTATGAAGAAGAAAAGCGGTATTATAGTAACAGCAGTCAGCTGTCTTCTGAAAGATTGGCCAGTAGTGGATACAGTCTTGAAGGGACATCTAAAAATGCAGAACCAAAAGATCAGAGTCCTCAAGATCCCATTGTTGAAGTTATAACACCTGAAGTGTACAATAACTATGGTGCAGATGGTTTCAAGGATTCATTTTCCGGGATCTGGTCTCGGAAATTGAGAATCAAAATAACTGGAAGAGATGGATTTGAGAAACTTGAAGTTAGAATTCCTGCTGGATTCTCGGACGGAATCACCAATATAGTTCCAGCTCTTAGGGGTGTAAACATCAAAGAATTGTTGAATGAGGCAGCTGATGAAATGGGAGGGAAGCAGCTGTTAGATTTTAATGACACAATGGGTGATAGAATTCAAGTTTTTCTAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PEP_mutase

Protein Analysis

439

Amino Acids

47.39

Weight (kDa)

5.23

Isoelectric Point (pI)

41.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEP_mutase	PF13714	37 - 271	4.7e-45	Phosphoenolpyruvate phosphomutase
ICL	PF00463	94 - 181	4.6e-13	Isocitrate lyase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43180 AT2G43180 AT2G43180 AT2G43180
fragaria_vesca	FvH4_4g11620 FvH4_4g11620
malus_domestica	MD04G1031000.v1.1
prunus_persica	Prupe.1G182700_v2.0.a1 Prupe.1G182700_v2.0.a1 Prupe.1G182700_v2.0.a1
pyrus_communis	pycom04g02620
rosa_chinensis	RchiOBHm_Chr4g0408741 RchiOBHm_Chr4g0408751
rosa_laevigata	RLG00000008633 RLG00000008634
rosa_multiflora	Rmu_sc0001258.1_g000006 Rmu_sc0005878.1_g000017 Rmu_sc0007333.1_g000009
rosa_roxburghii	Rroxscaffold_5G00353090
rosa_rugosa	Rorug04G0087300
rosa_samantha	Rh1DG149900 Rh4AG151500 Rh4AG151600 Rh4BG148700 Rh4BG148800 Rh4CG159600 Rh4CG159800 Rh4DG144700 Rh4DG144800
rosa_wichuraiana	Rw0G015170 Rw4G012420 Rw4G012440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1034
AciI	CCGC	7 cut(s)	41, 44, 47, 73, 408, 457, 907
AclWI	GGATC	4 cut(s)	13, 255, 1010, 1100
AcoI	YGGCCR	2 cut(s)	60, 948
AcsI	RAATTY	2 cut(s)	1158, 1299
AcuI	CTGAAG	1 cut(s)	1062
AfaI	GTAC	1 cut(s)	1049
AfeI	AGCGCT	1 cut(s)	69
AfiI	CCNNNNNNNGG	6 cut(s)	82, 218, 290, 771, 836, 1095
AflII	CTTAAG	1 cut(s)	863
AjnI	CCWGG	1 cut(s)	835
AloI	GAACNNNNNNTCC	2 cut(s)	579, 611
Alw26I	GTCTC	4 cut(s)	60, 131, 399, 1104
AlwI	GGATC	4 cut(s)	13, 255, 1010, 1100
Ama87I	CYCGRG	1 cut(s)	50
Aor51HI	AGCGCT	1 cut(s)	69
AoxI	GGCC	3 cut(s)	60, 106, 948
ApeKI	GCWGC	7 cut(s)	26, 194, 208, 467, 806, 1241, 1264
ApoI	RAATTY	2 cut(s)	1158, 1299
Asp700I	GAANNNNTTC	1 cut(s)	638
AspA2I	CCTAGG	1 cut(s)	874
AspLEI	GCGC	3 cut(s)	40, 70, 134
AspS9I	GGNCC	2 cut(s)	107, 871
AsuC2I	CCSGG	2 cut(s)	93, 1090
AsuHPI	GGTGA	4 cut(s)	92, 163, 1177, 1304
AvaI	CYCGRG	1 cut(s)	50
AvaII	GGWCC	1 cut(s)	871
AvrII	CCTAGG	1 cut(s)	874
BalI	TGGCCA	1 cut(s)	950
BauI	CACGAG	1 cut(s)	417
BbsI	GAAGAC	1 cut(s)	927
BbvI	GCAGC	7 cut(s)	38, 181, 195, 479, 793, 1253, 1276
BccI	CCATC	5 cut(s)	116, 290, 856, 1061, 1127
BciT130I	CCWGG	1 cut(s)	837
BciVI	GTATCC	3 cut(s)	731, 836, 953
BcnI	CCSGG	2 cut(s)	93, 1090
BcoDI	GTCTC	4 cut(s)	60, 131, 399, 1104
BfaI	CTAG	5 cut(s)	614, 659, 803, 875, 1313
BfoI	RGCGCY	3 cut(s)	41, 71, 135
BfrI	CTTAAG	1 cut(s)	863
BfuI	GTATCC	3 cut(s)	731, 836, 953
BlnI	CCTAGG	1 cut(s)	874
Bme1390I	CCNGG	3 cut(s)	93, 837, 1090
Bme18I	GGWCC	1 cut(s)	871
BmeT110I	CYCGRG	1 cut(s)	50
BmgT120I	GGNCC	2 cut(s)	107, 871
BmrFI	CCNGG	3 cut(s)	93, 837, 1090
BmsI	GCATC	5 cut(s)	444, 466, 514, 571, 598
BpiI	GAAGAC	1 cut(s)	927
BpmI	CTGGAG	2 cut(s)	104, 606
BpuEI	CTTGAG	2 cut(s)	746, 996
BpuMI	CCSGG	2 cut(s)	93, 1090
BsaI	GGTCTC	2 cut(s)	131, 1104
BsaJI	CCNNGG	4 cut(s)	92, 103, 342, 874
BsaXI	ACNNNNNCTCC	4 cut(s)	228, 258, 579, 609
Bsc4I	CCNNNNNNNGG	6 cut(s)	82, 218, 290, 771, 836, 1095
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	4 cut(s)	160, 174, 951, 1129
Bse3DI	GCAATG	2 cut(s)	327, 795
BseBI	CCWGG	1 cut(s)	837
BseDI	CCNNGG	4 cut(s)	92, 103, 342, 874
BseGI	GGATG	1 cut(s)	301
BseLI	CCNNNNNNNGG	6 cut(s)	82, 218, 290, 771, 836, 1095
BseMI	GCAATG	2 cut(s)	327, 795
BseMII	CTCAG	2 cut(s)	48, 142
BseNI	ACTGG	4 cut(s)	160, 174, 951, 1129
BseRI	GAGGAG	1 cut(s)	831
BseXI	GCAGC	7 cut(s)	38, 181, 195, 479, 793, 1253, 1276
BsgI	GTGCAG	1 cut(s)	1083
BshFI	GGCC	3 cut(s)	62, 108, 950
BsiHKCI	CYCGRG	1 cut(s)	50
BsiSI	CCGG	5 cut(s)	59, 76, 92, 153, 1089
BslFI	GGGAC	1 cut(s)	988
BslI	CCNNNNNNNGG	6 cut(s)	82, 218, 290, 771, 836, 1095
BsmAI	GTCTC	4 cut(s)	60, 131, 399, 1104
BsmFI	GGGAC	1 cut(s)	988
BsmI	GAATGC	3 cut(s)	322, 372, 572
BsnI	GGCC	3 cut(s)	62, 108, 950
Bso31I	GGTCTC	2 cut(s)	131, 1104
BsoBI	CYCGRG	1 cut(s)	50
Bsp1407I	TGTACA	1 cut(s)	1047
Bsp143I	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
BspACI	CCGC	7 cut(s)	41, 44, 47, 73, 408, 457, 907
BspANI	GGCC	3 cut(s)	62, 108, 950
BspCNI	CTCAG	2 cut(s)	47, 141
BspPI	GGATC	4 cut(s)	13, 255, 1010, 1100
BspQI	GCTCTTC	1 cut(s)	852
BspTI	CTTAAG	1 cut(s)	863
BspTNI	GGTCTC	2 cut(s)	131, 1104
BsrDI	GCAATG	2 cut(s)	327, 795
BsrFI	RCCGGY	1 cut(s)	152
BsrGI	TGTACA	1 cut(s)	1047
BsrI	ACTGG	4 cut(s)	160, 174, 951, 1129
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	4 cut(s)	92, 103, 342, 874
BssMI	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
BssSI	CACGAG	1 cut(s)	417
BssT1I	CCWWGG	3 cut(s)	103, 342, 874
Bst2BI	CACGAG	1 cut(s)	417
Bst2UI	CCWGG	1 cut(s)	837
Bst4CI	ACNGT	3 cut(s)	308, 887, 965
Bst6I	CTCTTC	2 cut(s)	852, 1123
BstAFI	CTTAAG	1 cut(s)	863
BstAPI	GCANNNNNTGC	2 cut(s)	476, 806
BstAUI	TGTACA	1 cut(s)	1047
BstC8I	GCNNGC	6 cut(s)	115, 154, 361, 370, 406, 845
BstDEI	CTNAG	3 cut(s)	34, 128, 1205
BstF5I	GGATG	1 cut(s)	301
BstH2I	RGCGCY	3 cut(s)	41, 71, 135
BstHHI	GCGC	3 cut(s)	40, 70, 134
BstKTI	GATC	7 cut(s)	21, 250, 388, 521, 1003, 1018, 1095
BstMAI	GTCTC	4 cut(s)	60, 131, 399, 1104
BstMBI	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
BstMWI	GCNNNNNNNGC	8 cut(s)	35, 46, 114, 365, 369, 476, 533, 806
BstNI	CCWGG	1 cut(s)	837
BstNSI	RCATGY	1 cut(s)	847
BstSCI	CCNGG	3 cut(s)	91, 835, 1088
BstV1I	GCAGC	7 cut(s)	38, 181, 195, 479, 793, 1253, 1276
BstV2I	GAAGAC	1 cut(s)	927
BstX2I	RGATCY	3 cut(s)	18, 1015, 1092
BstYI	RGATCY	3 cut(s)	18, 1015, 1092
BsuI	GTATCC	3 cut(s)	731, 836, 953
BsuRI	GGCC	3 cut(s)	62, 108, 950
BtsCI	GGATG	1 cut(s)	301
BtsI	GCAGTG	1 cut(s)	543
BtsIMutI	CAGTG	2 cut(s)	181, 543
Cac8I	GCNNGC	6 cut(s)	115, 154, 361, 370, 406, 845
CfoI	GCGC	3 cut(s)	40, 70, 134
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	2 cut(s)	107, 871
CseI	GACGC	1 cut(s)	806
Csp6I	GTAC	1 cut(s)	1048
CspCI	CAANNNNNGTGG	2 cut(s)	747, 782
CviAII	CATG	1 cut(s)	844
CviQI	GTAC	1 cut(s)	1048
DdeI	CTNAG	3 cut(s)	34, 128, 1205
DpnI	GATC	7 cut(s)	20, 249, 387, 520, 1002, 1017, 1094
DpnII	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
EaeI	YGGCCR	2 cut(s)	60, 948
Eam1104I	CTCTTC	2 cut(s)	852, 1123
EarI	CTCTTC	2 cut(s)	852, 1123
EciI	GGCGGA	1 cut(s)	62
Eco130I	CCWWGG	3 cut(s)	103, 342, 874
Eco31I	GGTCTC	2 cut(s)	131, 1104
Eco32I	GATATC	1 cut(s)	189
Eco47I	GGWCC	1 cut(s)	871
Eco47III	AGCGCT	1 cut(s)	69
Eco57I	CTGAAG	1 cut(s)	1062
Eco88I	CYCGRG	1 cut(s)	50
EcoO109I	RGGNCCY	2 cut(s)	107, 871
EcoRI	GAATTC	2 cut(s)	1158, 1299
EcoRII	CCWGG	1 cut(s)	835
EcoRV	GATATC	1 cut(s)	189
EcoT14I	CCWWGG	3 cut(s)	103, 342, 874
ErhI	CCWWGG	3 cut(s)	103, 342, 874
FaeI	CATG	1 cut(s)	847
FaiI	YATR	9 cut(s)	414, 652, 680, 845, 894, 915, 1034, 1059, 1193
FalI	AAGNNNNNCTT	2 cut(s)	540, 572
FaqI	GGGAC	1 cut(s)	988
FatI	CATG	1 cut(s)	843
FokI	GGATG	1 cut(s)	308
FspBI	CTAG	5 cut(s)	614, 659, 803, 875, 1313
GlaI	GCGC	3 cut(s)	39, 69, 133
GsuI	CTGGAG	2 cut(s)	104, 606
HaeII	RGCGCY	3 cut(s)	41, 71, 135
HaeIII	GGCC	3 cut(s)	62, 108, 950
HapII	CCGG	5 cut(s)	59, 76, 92, 153, 1089
HgaI	GACGC	1 cut(s)	806
HhaI	GCGC	3 cut(s)	40, 70, 134
Hin1II	CATG	1 cut(s)	847
Hin6I	GCGC	3 cut(s)	38, 68, 132
HinP1I	GCGC	3 cut(s)	38, 68, 132
HindIII	AAGCTT	1 cut(s)	137
HinfI	GANTC	7 cut(s)	53, 79, 1006, 1079, 1113, 1170, 1182
HpaII	CCGG	5 cut(s)	59, 76, 92, 153, 1089
HphI	GGTGA	4 cut(s)	92, 163, 1177, 1304
Hpy166II	GTNNAC	3 cut(s)	100, 1048, 1216
Hpy188I	TCNGA	8 cut(s)	483, 499, 523, 815, 940, 1005, 1104, 1177
Hpy188III	TCNNGA	6 cut(s)	83, 331, 719, 968, 1013, 1313
Hpy8I	GTNNAC	3 cut(s)	100, 1048, 1216
HpyAV	CCTTC	3 cut(s)	628, 680, 965
HpyCH4III	ACNGT	3 cut(s)	308, 887, 965
HpyCH4IV	ACGT	1 cut(s)	327
HpyCH4V	TGCA	5 cut(s)	320, 611, 793, 989, 1064
HpyF10VI	GCNNNNNNNGC	8 cut(s)	35, 46, 114, 365, 369, 476, 533, 806
HpyF3I	CTNAG	3 cut(s)	34, 128, 1205
HpySE526I	ACGT	1 cut(s)	327
Hsp92II	CATG	1 cut(s)	847
HspAI	GCGC	3 cut(s)	38, 68, 132
KroI	GCCGGC	1 cut(s)	152
KroNI	GCCGGC	1 cut(s)	154
Kzo9I	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
LguI	GCTCTTC	1 cut(s)	852
LmnI	GCTCC	2 cut(s)	75, 587
Lsp1109I	GCAGC	7 cut(s)	38, 181, 195, 479, 793, 1253, 1276
LweI	GCATC	5 cut(s)	444, 466, 514, 571, 598
MaeI	CTAG	5 cut(s)	614, 659, 803, 875, 1313
MaeII	ACGT	1 cut(s)	327
MaeIII	GTNAC	2 cut(s)	308, 917
MalI	GATC	7 cut(s)	20, 249, 387, 520, 1002, 1017, 1094
MboI	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
MboII	GAAGA	8 cut(s)	395, 560, 638, 869, 908, 911, 927, 1140
MflI	RGATCY	3 cut(s)	18, 1015, 1092
MlsI	TGGCCA	1 cut(s)	950
MluCI	AATT	8 cut(s)	261, 279, 507, 744, 1106, 1158, 1226, 1299
MluNI	TGGCCA	1 cut(s)	950
MlyI	GAGTC	2 cut(s)	62, 1015
MmeI	TCCRAC	1 cut(s)	715
Mox20I	TGGCCA	1 cut(s)	950
MroNI	GCCGGC	1 cut(s)	152
MroXI	GAANNNNTTC	1 cut(s)	638
MscI	TGGCCA	1 cut(s)	950
MseI	TTAA	2 cut(s)	864, 1278
Msp20I	TGGCCA	1 cut(s)	950
MspA1I	CMGCKG	3 cut(s)	931, 1244, 1267
MspCI	CTTAAG	1 cut(s)	863
MspI	CCGG	5 cut(s)	59, 76, 92, 153, 1089
MspR9I	CCNGG	3 cut(s)	93, 837, 1090
Mva1269I	GAATGC	3 cut(s)	322, 372, 572
MvaI	CCWGG	1 cut(s)	837
MwoI	GCNNNNNNNGC	8 cut(s)	35, 46, 114, 365, 369, 476, 533, 806
NaeI	GCCGGC	1 cut(s)	154
NciI	CCSGG	2 cut(s)	93, 1090
NdeII	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
NgoMIV	GCCGGC	1 cut(s)	152
NlaIII	CATG	1 cut(s)	847
NspI	RCATGY	1 cut(s)	847
PaeI	GCATGC	1 cut(s)	847
PciSI	GCTCTTC	1 cut(s)	852
PctI	GAATGC	3 cut(s)	322, 372, 572
PdiI	GCCGGC	1 cut(s)	154
PdmI	GAANNNNTTC	1 cut(s)	638
PfeI	GAWTC	5 cut(s)	79, 1079, 1113, 1170, 1182
PfoI	TCCNGGA	1 cut(s)	1088
PleI	GAGTC	2 cut(s)	61, 1014
PpsI	GAGTC	2 cut(s)	61, 1014
PpuMI	RGGWCCY	1 cut(s)	871
PsiI	TTATAA	1 cut(s)	1034
Psp5II	RGGWCCY	1 cut(s)	871
Psp6I	CCWGG	1 cut(s)	835
PspGI	CCWGG	1 cut(s)	835
PspPI	GGNCC	2 cut(s)	107, 871
PspPPI	RGGWCCY	1 cut(s)	871
PsuI	RGATCY	3 cut(s)	18, 1015, 1092
PvuII	CAGCTG	3 cut(s)	931, 1244, 1267
RsaI	GTAC	1 cut(s)	1049
RsaNI	GTAC	1 cut(s)	1048
SapI	GCTCTTC	1 cut(s)	852
SaqAI	TTAA	2 cut(s)	864, 1278
Sau3AI	GATC	7 cut(s)	18, 247, 385, 518, 1000, 1015, 1092
Sau96I	GGNCC	2 cut(s)	107, 871
SchI	GAGTC	2 cut(s)	62, 1015
ScrFI	CCNGG	3 cut(s)	93, 837, 1090
SfaNI	GCATC	5 cut(s)	444, 466, 514, 571, 598
SinI	GGWCC	1 cut(s)	871
SmlI	CTYRAG	3 cut(s)	725, 863, 1011
SmoI	CTYRAG	3 cut(s)	725, 863, 1011
SphI	GCATGC	1 cut(s)	847
Sse9I	AATT	8 cut(s)	261, 279, 507, 744, 1106, 1158, 1226, 1299
SsiI	CCGC	7 cut(s)	41, 44, 47, 73, 408, 457, 907
SspMI	CTAG	5 cut(s)	614, 659, 803, 875, 1313
StyD4I	CCNGG	3 cut(s)	91, 835, 1088
StyI	CCWWGG	3 cut(s)	103, 342, 874
TaaI	ACNGT	3 cut(s)	308, 887, 965
TaiI	ACGT	1 cut(s)	330
TaqI	TCGA	1 cut(s)	185
TasI	AATT	8 cut(s)	261, 279, 507, 744, 1106, 1158, 1226, 1299
TatI	WGTACW	1 cut(s)	1047
TauI	GCSGC	3 cut(s)	43, 46, 49
TfiI	GAWTC	5 cut(s)	79, 1079, 1113, 1170, 1182
Tru1I	TTAA	2 cut(s)	864, 1278
Tru9I	TTAA	2 cut(s)	864, 1278
TscAI	CASTG	2 cut(s)	181, 543
TseI	GCWGC	7 cut(s)	26, 194, 208, 467, 806, 1241, 1264
TspDTI	ATGAA	6 cut(s)	338, 592, 647, 909, 1071, 1263
TspGWI	ACGGA	2 cut(s)	249, 1194
TspRI	CASTG	2 cut(s)	181, 543
Vha464I	CTTAAG	1 cut(s)	863
VpaK11BI	GGWCC	1 cut(s)	871
XapI	RAATTY	2 cut(s)	1158, 1299
XbaI	TCTAGA	1 cut(s)	1312
XceI	RCATGY	1 cut(s)	847
XmaJI	CCTAGG	1 cut(s)	874
XmnI	GAANNNNTTC	1 cut(s)	638
XspI	CTAG	5 cut(s)	614, 659, 803, 875, 1313

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.