Rw4G012420

Phosphoenolpyruvate phosphomutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004495
Physical Location & Seq
Forward (+)
29176385 .. 29181888
5504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012420.1

Sequence Viewer

Length: 1494 bp
ATGAGTTGTGTGCTGCAGCAGCTGTATCCTCAGGTGGGTAGCAGAGACTGCAGTGTGTCTCTTCCCCACAGCTCCTGTCTCAATGCCAGCAGCTCGCCTTCAAACCTTACATTCGGGTCACGTCTTCATCAACGATCACTAACACTGCCCCACAAGTCAAATGCGTCTCCTTTTCTTACCACAAGGATCCTCGCAGCTTACTCAGGAGACTCAGCGCCGCCGCCCGAGTCTCCTGCCAAAGCGCTTCGCCGGATTCTGGAGTTGCCGGGGGTTCACCAAGGCCCTGCTTGCTTTGATGGTCTCAGCGCCAAGCTTGTCGAAAGAGCTGGCTTCCAGTATTGCTTCACCAGTGGATTTTCGATATCAGCTGCTAGATTAGGGCTGCCAGATACAGGTTTTCTATCCTACGGAGAAATGGTGGACCAGGGGCAACAAATTACCCAAGCTGTGTCAATTCCTGTCATTGGGGATGGCGATAACGGTTACGGGAATGCAATGAATGTCAAGAGAACTGTCAAGGGATATATTAGAGCTGGCTTTGCTGGGATTCTGCTTGAAGATCAGGTGTCTCCAAAAGCCTGCGGTCATACACAAGGCAGGAAAGTGGTGTCAAGGGAGGAAGCTGTGATGCGGATAAAAGCAGCAGTTGATGCTCGGAAAGAGAGTGGCTCTGACATTGTAATTGTAGCACGATCTGATTCTCGTCAAGCAGTGTCTTTGGAAGAAGCACTTTGGAGGTCAAGGGCATTCGCTGATGCTGGAGCAGATGTTCTTTTCATTGATGCACTAGCCTCAAAAGAAGAAATGAAGGCTTTCTGTGGAGTATATCCCCTAGTTCCAAAAATGGCGAATATGCTTGAAGGAGGGGGGAAAACACCAATACTCAATCCTCTTGAACTTGAGGAAGTTGGATACAAACTTGTGGCTTATCCACTTTCCTTGATTGGGGTATCTATTCAAGCAATGCAGGACGCACTAGCTGCTATCAGAGGAGGTCGTATCCCTCCACCTGGAAGCATGCCATCTTTTGAAGAGGTTAAGGAGGTCCTAGGTTTCAACAGTTATTATGAAGAAGAAAAGCGGTATTATAGTAACAGCAGTCAGCTGTCTTCTGAAAGATTGGCCAGTAGTGTATACAGTCTTGAAGGGACATCTAAAAATGCAGAACCAAAAGATCAGAGTCCTCAAGATCCCATTGTTGAAGTTATAACACCTGAAGTGTACAATAACTATGGTGCAGATGGTTCCAAGGATTCATTTTCCGGGATCTGGTCTCGGAAGTTGAGAATCAAAATAACCGGAAGAGATGGATTTGAGAAACTTGATGTTAGTATTCCTGCTGGATTCTTGGACGGAATCACCAATATAGTTCCAGCTCTTGGGGGTGTAAACATCAAAGAATTGTTGAATGAGGCAGCTGATGGAATGGGAGGGAAGCAGCTATTAGATTTTAATGACGCAATGGGTGATAGAATTCAAGTTTTTCTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_mutase

Protein Analysis

497

Amino Acids

53.41

Weight (kDa)

5.53

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP_mutase PF13714 95 - 329 3e-45 Phosphoenolpyruvate phosphomutase
ICL PF00463 152 - 239 1.2e-12 Isocitrate lyase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1208
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccI GTMKAC 1 cut(s) 1134
AciI CCGC 5 cut(s) 218, 221, 582, 631, 1081
AclWI GGATC 4 cut(s) 181, 194, 1184, 1274
AcoI YGGCCR 1 cut(s) 1122
AcsI RAATTY 1 cut(s) 1473
AcuI CTGAAG 1 cut(s) 1236
AfaI GTAC 1 cut(s) 1223
AfeI AGCGCT 1 cut(s) 243
AfiI CCNNNNNNNGG 8 cut(s) 35, 256, 392, 464, 945, 1010, 1269, 1379
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 2 cut(s) 423, 1009
AloI GAACNNNNNNTCC 2 cut(s) 753, 785
Alw26I GTCTC 9 cut(s) 39, 63, 83, 171, 201, 234, 305, 573, 1278
AlwI GGATC 4 cut(s) 181, 194, 1184, 1274
AlwNI CAGNNNCTG 3 cut(s) 22, 48, 75
Ama87I CYCGRG 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 243
AoxI GGCC 2 cut(s) 280, 1122
ApoI RAATTY 1 cut(s) 1473
Asp700I GAANNNNTTC 1 cut(s) 812
AspA2I CCTAGG 1 cut(s) 1048
AspLEI GCGC 3 cut(s) 217, 244, 308
AspS9I GGNCC 3 cut(s) 281, 421, 1045
AsuC2I CCSGG 2 cut(s) 267, 1264
AsuHPI GGTGA 4 cut(s) 266, 337, 1351, 1478
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 2 cut(s) 421, 1045
AvrII CCTAGG 1 cut(s) 1048
AxyI CCTNAGG 1 cut(s) 30
BalI TGGCCA 1 cut(s) 1124
BamHI GGATCC 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 116, 1101
BccI CCATC 6 cut(s) 290, 464, 1030, 1235, 1301, 1415
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 425, 1011
BciVI GTATCC 3 cut(s) 36, 905, 1010
BcnI CCSGG 2 cut(s) 267, 1264
BcoDI GTCTC 9 cut(s) 39, 63, 83, 171, 201, 234, 305, 573, 1278
BfaI CTAG 6 cut(s) 372, 788, 833, 977, 1049, 1487
BfmI CTRYAG 2 cut(s) 14, 49
BfoI RGCGCY 3 cut(s) 218, 245, 309
BfuI GTATCC 3 cut(s) 36, 905, 1010
BlnI CCTAGG 1 cut(s) 1048
Bme1390I CCNGG 4 cut(s) 267, 425, 1011, 1264
Bme18I GGWCC 2 cut(s) 421, 1045
BmeT110I CYCGRG 1 cut(s) 224
BmgBI CACGTC 1 cut(s) 122
BmgT120I GGNCC 3 cut(s) 281, 421, 1045
BmiI GGNNCC 2 cut(s) 188, 1246
BmrFI CCNGG 4 cut(s) 267, 425, 1011, 1264
BmsI GCATC 4 cut(s) 618, 640, 745, 772
BpiI GAAGAC 2 cut(s) 116, 1101
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 2 cut(s) 278, 780
BpuEI CTTGAG 2 cut(s) 920, 1170
BpuMI CCSGG 2 cut(s) 267, 1264
BsaI GGTCTC 2 cut(s) 305, 1278
BsaJI CCNNGG 5 cut(s) 266, 277, 424, 1048, 1248
BsaWI WCCGGW 1 cut(s) 1298
BsaXI ACNNNNNCTCC 4 cut(s) 402, 432, 753, 783
Bsc4I CCNNNNNNNGG 8 cut(s) 35, 256, 392, 464, 945, 1010, 1269, 1379
Bse1I ACTGG 3 cut(s) 334, 348, 1125
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 3 cut(s) 501, 969, 1467
BseBI CCWGG 2 cut(s) 425, 1011
BseDI CCNNGG 5 cut(s) 266, 277, 424, 1048, 1248
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 8 cut(s) 35, 256, 392, 464, 945, 1010, 1269, 1379
BseMI GCAATG 3 cut(s) 501, 969, 1467
BseMII CTCAG 4 cut(s) 44, 216, 225, 316
BseNI ACTGG 3 cut(s) 334, 348, 1125
BseRI GAGGAG 1 cut(s) 1005
BseYI CCCAGC 1 cut(s) 542
BsgI GTGCAG 1 cut(s) 1257
BshFI GGCC 2 cut(s) 282, 1124
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 4 cut(s) 250, 266, 1263, 1299
BslFI GGGAC 1 cut(s) 1162
BslI CCNNNNNNNGG 8 cut(s) 35, 256, 392, 464, 945, 1010, 1269, 1379
BsmAI GTCTC 9 cut(s) 39, 63, 83, 171, 201, 234, 305, 573, 1278
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 1162
BsmI GAATGC 2 cut(s) 496, 746
BsnI GGCC 2 cut(s) 282, 1124
Bso31I GGTCTC 2 cut(s) 305, 1278
BsoBI CYCGRG 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 1221
Bsp143I GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
BspACI CCGC 5 cut(s) 218, 221, 582, 631, 1081
BspANI GGCC 2 cut(s) 282, 1124
BspCNI CTCAG 4 cut(s) 43, 215, 224, 315
BspLI GGNNCC 2 cut(s) 188, 1246
BspMAI CTGCAG 2 cut(s) 18, 53
BspPI GGATC 4 cut(s) 181, 194, 1184, 1274
BspTNI GGTCTC 2 cut(s) 305, 1278
BsrDI GCAATG 3 cut(s) 501, 969, 1467
BsrGI TGTACA 1 cut(s) 1221
BsrI ACTGG 3 cut(s) 334, 348, 1125
BssECI CCNNGG 5 cut(s) 266, 277, 424, 1048, 1248
BssMI GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
BssNAI GTATAC 1 cut(s) 1135
BssT1I CCWWGG 3 cut(s) 277, 1048, 1248
Bst1107I GTATAC 1 cut(s) 1135
Bst2UI CCWGG 2 cut(s) 425, 1011
Bst4CI ACNGT 4 cut(s) 482, 514, 1061, 1139
Bst6I CTCTTC 3 cut(s) 66, 1026, 1297
BstAPI GCANNNNNTGC 3 cut(s) 48, 650, 980
BstAUI TGTACA 1 cut(s) 1221
BstC8I GCNNGC 7 cut(s) 88, 95, 289, 328, 535, 580, 1019
BstDEI CTNAG 4 cut(s) 30, 202, 211, 302
BstF5I GGATG 1 cut(s) 475
BstH2I RGCGCY 3 cut(s) 218, 245, 309
BstHHI GCGC 3 cut(s) 217, 244, 308
BstKTI GATC 7 cut(s) 137, 189, 562, 695, 1177, 1192, 1269
BstMAI GTCTC 9 cut(s) 39, 63, 83, 171, 201, 234, 305, 573, 1278
BstMBI GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
BstMWI GCNNNNNNNGC 6 cut(s) 19, 48, 288, 539, 650, 980
BstNI CCWGG 2 cut(s) 425, 1011
BstNSI RCATGY 1 cut(s) 1021
BstSCI CCNGG 4 cut(s) 265, 423, 1009, 1262
BstSFI CTRYAG 2 cut(s) 14, 49
BstV2I GAAGAC 2 cut(s) 116, 1101
BstX2I RGATCY 3 cut(s) 186, 1189, 1266
BstYI RGATCY 3 cut(s) 186, 1189, 1266
BstZ17I GTATAC 1 cut(s) 1135
Bsu36I CCTNAGG 1 cut(s) 30
BsuI GTATCC 3 cut(s) 36, 905, 1010
BsuRI GGCC 2 cut(s) 282, 1124
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 1 cut(s) 475
BtsI GCAGTG 3 cut(s) 58, 143, 717
BtsIMutI CAGTG 4 cut(s) 58, 143, 355, 717
Cac8I GCNNGC 7 cut(s) 88, 95, 289, 328, 535, 580, 1019
CaiI CAGNNNCTG 3 cut(s) 22, 48, 75
CfoI GCGC 3 cut(s) 217, 244, 308
Cfr13I GGNCC 3 cut(s) 281, 421, 1045
CseI GACGC 3 cut(s) 153, 980, 1466
Csp6I GTAC 1 cut(s) 1222
CspCI CAANNNNNGTGG 2 cut(s) 921, 956
CviAII CATG 1 cut(s) 1018
CviQI GTAC 1 cut(s) 1222
DdeI CTNAG 4 cut(s) 30, 202, 211, 302
DpnI GATC 7 cut(s) 136, 188, 561, 694, 1176, 1191, 1268
DpnII GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
EaeI YGGCCR 1 cut(s) 1122
Eam1104I CTCTTC 3 cut(s) 66, 1026, 1297
EarI CTCTTC 3 cut(s) 66, 1026, 1297
Eco130I CCWWGG 3 cut(s) 277, 1048, 1248
Eco31I GGTCTC 2 cut(s) 305, 1278
Eco32I GATATC 1 cut(s) 363
Eco47I GGWCC 2 cut(s) 421, 1045
Eco47III AGCGCT 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 1236
Eco81I CCTNAGG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 224
EcoO109I RGGNCCY 2 cut(s) 281, 1045
EcoRI GAATTC 1 cut(s) 1473
EcoRII CCWGG 2 cut(s) 423, 1009
EcoRV GATATC 1 cut(s) 363
EcoT14I CCWWGG 3 cut(s) 277, 1048, 1248
ErhI CCWWGG 3 cut(s) 277, 1048, 1248
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 1 cut(s) 1021
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 1162
FatI CATG 1 cut(s) 1017
FblI GTMKAC 1 cut(s) 1134
FokI GGATG 1 cut(s) 482
FspBI CTAG 6 cut(s) 372, 788, 833, 977, 1049, 1487
GlaI GCGC 3 cut(s) 216, 243, 307
GsaI CCCAGC 1 cut(s) 546
GsuI CTGGAG 2 cut(s) 278, 780
HaeII RGCGCY 3 cut(s) 218, 245, 309
HaeIII GGCC 2 cut(s) 282, 1124
HapII CCGG 4 cut(s) 250, 266, 1263, 1299
HgaI GACGC 3 cut(s) 153, 980, 1466
HhaI GCGC 3 cut(s) 217, 244, 308
Hin1II CATG 1 cut(s) 1021
Hin6I GCGC 3 cut(s) 215, 242, 306
HinP1I GCGC 3 cut(s) 215, 242, 306
HindIII AAGCTT 1 cut(s) 311
HpaII CCGG 4 cut(s) 250, 266, 1263, 1299
HphI GGTGA 4 cut(s) 266, 337, 1351, 1478
Hpy166II GTNNAC 5 cut(s) 274, 421, 1135, 1222, 1390
Hpy188I TCNGA 7 cut(s) 657, 673, 697, 989, 1114, 1179, 1278
Hpy188III TCNNGA 7 cut(s) 204, 257, 505, 893, 1142, 1187, 1487
Hpy8I GTNNAC 5 cut(s) 274, 421, 1135, 1222, 1390
HpyAV CCTTC 4 cut(s) 108, 802, 854, 1139
HpyCH4III ACNGT 4 cut(s) 482, 514, 1061, 1139
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 7 cut(s) 16, 51, 494, 785, 967, 1163, 1238
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 48, 288, 539, 650, 980
HpyF3I CTNAG 4 cut(s) 30, 202, 211, 302
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 1 cut(s) 1021
HspAI GCGC 3 cut(s) 215, 242, 306
Kzo9I GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
LmnI GCTCC 2 cut(s) 77, 761
LweI GCATC 4 cut(s) 618, 640, 745, 772
MaeI CTAG 6 cut(s) 372, 788, 833, 977, 1049, 1487
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 3 cut(s) 117, 482, 1091
MalI GATC 7 cut(s) 136, 188, 561, 694, 1176, 1191, 1268
MboI GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
MflI RGATCY 3 cut(s) 186, 1189, 1266
MlsI TGGCCA 1 cut(s) 1124
MluCI AATT 5 cut(s) 435, 453, 681, 1400, 1473
MluNI TGGCCA 1 cut(s) 1124
MlyI GAGTC 3 cut(s) 203, 236, 1189
MmeI TCCRAC 1 cut(s) 889
Mox20I TGGCCA 1 cut(s) 1124
MroXI GAANNNNTTC 1 cut(s) 812
MscI TGGCCA 1 cut(s) 1124
MseI TTAA 2 cut(s) 1038, 1452
Msp20I TGGCCA 1 cut(s) 1124
MspA1I CMGCKG 4 cut(s) 22, 368, 1105, 1418
MspI CCGG 4 cut(s) 250, 266, 1263, 1299
MspR9I CCNGG 4 cut(s) 267, 425, 1011, 1264
Mva1269I GAATGC 2 cut(s) 496, 746
MvaI CCWGG 2 cut(s) 425, 1011
MwoI GCNNNNNNNGC 6 cut(s) 19, 48, 288, 539, 650, 980
NciI CCSGG 2 cut(s) 267, 1264
NdeII GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
NlaIII CATG 1 cut(s) 1021
NlaIV GGNNCC 2 cut(s) 188, 1246
NmuCI GTSAC 1 cut(s) 117
NspI RCATGY 1 cut(s) 1021
PaeI GCATGC 1 cut(s) 1021
PctI GAATGC 2 cut(s) 496, 746
PdmI GAANNNNTTC 1 cut(s) 812
PfeI GAWTC 7 cut(s) 253, 547, 698, 1253, 1287, 1344, 1356
PflMI CCANNNNNTGG 1 cut(s) 1379
PfoI TCCNGGA 1 cut(s) 1262
PleI GAGTC 3 cut(s) 203, 235, 1188
PpsI GAGTC 3 cut(s) 203, 235, 1188
PpuMI RGGWCCY 1 cut(s) 1045
PsiI TTATAA 1 cut(s) 1208
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 2 cut(s) 423, 1009
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 2 cut(s) 423, 1009
PspN4I GGNNCC 2 cut(s) 188, 1246
PspPI GGNCC 3 cut(s) 281, 421, 1045
PspPPI RGGWCCY 1 cut(s) 1045
PstI CTGCAG 2 cut(s) 18, 53
PstNI CAGNNNCTG 3 cut(s) 22, 48, 75
PsuI RGATCY 3 cut(s) 186, 1189, 1266
PvuII CAGCTG 4 cut(s) 22, 368, 1105, 1418
RsaI GTAC 1 cut(s) 1223
RsaNI GTAC 1 cut(s) 1222
SaqAI TTAA 2 cut(s) 1038, 1452
Sau3AI GATC 7 cut(s) 134, 186, 559, 692, 1174, 1189, 1266
Sau96I GGNCC 3 cut(s) 281, 421, 1045
SchI GAGTC 3 cut(s) 203, 236, 1189
ScrFI CCNGG 4 cut(s) 267, 425, 1011, 1264
SfaNI GCATC 4 cut(s) 618, 640, 745, 772
SfcI CTRYAG 2 cut(s) 14, 49
SinI GGWCC 2 cut(s) 421, 1045
SmlI CTYRAG 2 cut(s) 899, 1185
SmoI CTYRAG 2 cut(s) 899, 1185
SphI GCATGC 1 cut(s) 1021
Sse9I AATT 5 cut(s) 435, 453, 681, 1400, 1473
SsiI CCGC 5 cut(s) 218, 221, 582, 631, 1081
SspMI CTAG 6 cut(s) 372, 788, 833, 977, 1049, 1487
StyD4I CCNGG 4 cut(s) 265, 423, 1009, 1262
StyI CCWWGG 3 cut(s) 277, 1048, 1248
TaaI ACNGT 4 cut(s) 482, 514, 1061, 1139
TaiI ACGT 1 cut(s) 124
TaqI TCGA 2 cut(s) 318, 359
TasI AATT 5 cut(s) 435, 453, 681, 1400, 1473
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 2 cut(s) 220, 223
TfiI GAWTC 7 cut(s) 253, 547, 698, 1253, 1287, 1344, 1356
Tru1I TTAA 2 cut(s) 1038, 1452
Tru9I TTAA 2 cut(s) 1038, 1452
TscAI CASTG 4 cut(s) 58, 150, 355, 717
TseFI GTSAC 1 cut(s) 117
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 6 cut(s) 116, 512, 766, 821, 1083, 1245
TspGWI ACGGA 2 cut(s) 423, 1368
TspRI CASTG 4 cut(s) 58, 150, 355, 717
Van91I CCANNNNNTGG 1 cut(s) 1379
VpaK11BI GGWCC 2 cut(s) 421, 1045
XapI RAATTY 1 cut(s) 1473
XbaI TCTAGA 1 cut(s) 1486
XceI RCATGY 1 cut(s) 1021
XmaJI CCTAGG 1 cut(s) 1048
XmiI GTMKAC 1 cut(s) 1134
XmnI GAANNNNTTC 1 cut(s) 812
XspI CTAG 6 cut(s) 372, 788, 833, 977, 1049, 1487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.