AT2G44525

Protein of unknown function (DUF498/DUF598)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005311
Physical Location & Seq
Forward (+)
18382070 .. 18383513
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44525.1

Sequence Viewer

Length: 513 bp
ATGGCGATGAGGTTGAAATCAATGGCGACTTTGCCGAAATTGATTCAAAGTATGAGAAAAGAAGTTCCGAAGCATTCTAATCCTGTTTTGCCCTCTCTGAGACGAGCTTTTTCCCTCTATGATCAGATCAATCTCATTGACAATGTTCCTGAAGACCAGCTTCGCTTCCAAGAATTTAATGATACGAGTTTCACTGTGAATGGAGTCAAATACGAAGGTAGCTTACTCTGTGTTGGGAATCTGTTAATGTCATGGAGTCCTCGTAAATTTTCAGAGATCACCACTGATAGCTTGTCTATCTTCCAGACAGTTCGACCTATTCCAGAGCTCTTGATTGTTGGTTGTGGAAGAGACATTCACCCGGTAACTCCCGAGGTCCGTCAGTTTGTCAAGTCCCTTGGCATGAAACTAGAAACAGTTGATTCTAGAAATGCTGCATCAACGTACAACATTCTGAATGAAGAAGGAAGAGTCGTTGCTGCGGCATTGCTTCCATATGGAGTTACATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

170

Amino Acids

19.06

Weight (kDa)

8.6

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 57 - 164 6.8e-32 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 482
AcsI RAATTY 2 cut(s) 173, 266
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 1 cut(s) 446
AgsI TTSAA 2 cut(s) 16, 47
AluBI AGCT 5 cut(s) 107, 160, 222, 291, 328
AluI AGCT 5 cut(s) 107, 160, 222, 291, 328
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 2 cut(s) 94, 345
Ama87I CYCGRG 1 cut(s) 371
ApeKI GCWGC 2 cut(s) 434, 479
ApoI RAATTY 2 cut(s) 173, 266
AspS9I GGNCC 1 cut(s) 376
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 2 cut(s) 271, 350
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 207, 239
BbsI GAAGAC 1 cut(s) 159
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 421, 466
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 94, 345
BfaI CTAG 2 cut(s) 410, 426
BisI GCNGC 3 cut(s) 435, 480, 483
BlsI GCNGC 3 cut(s) 436, 481, 484
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 376
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 376
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 446
BpiI GAAGAC 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 372, 397
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bse3DI GCAATG 1 cut(s) 485
BseDI CCNNGG 2 cut(s) 372, 397
BseGI GGATG 1 cut(s) 506
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 89
BseXI GCAGC 2 cut(s) 421, 466
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 1 cut(s) 379
BsmAI GTCTC 2 cut(s) 94, 345
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 3 cut(s) 121, 126, 276
BspACI CCGC 1 cut(s) 482
BspCNI CTCAG 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 372, 397
BssMI GATC 3 cut(s) 121, 126, 276
BssT1I CCWWGG 1 cut(s) 397
Bst4CI ACNGT 3 cut(s) 196, 310, 418
Bst6I CTCTTC 2 cut(s) 343, 463
BstDEI CTNAG 1 cut(s) 98
BstF5I GGATG 1 cut(s) 506
BstKTI GATC 3 cut(s) 124, 129, 279
BstMAI GTCTC 2 cut(s) 94, 345
BstMBI GATC 3 cut(s) 121, 126, 276
BstSCI CCNGG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 421, 466
BstV2I GAAGAC 1 cut(s) 159
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 506
BtsIMutI CAGTG 2 cut(s) 192, 282
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 1 cut(s) 445
CviAII CATG 2 cut(s) 252, 403
CviJI RGCY 5 cut(s) 107, 160, 222, 291, 328
CviKI_1 RGCY 5 cut(s) 107, 160, 222, 291, 328
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 1 cut(s) 98
DpnI GATC 3 cut(s) 123, 128, 278
DpnII GATC 3 cut(s) 121, 126, 276
Eam1104I CTCTTC 2 cut(s) 343, 463
EarI CTCTTC 2 cut(s) 343, 463
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 376
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 397
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 2 cut(s) 255, 406
FaiI YATR 6 cut(s) 53, 120, 253, 404, 496, 498
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 1 cut(s) 379
FatI CATG 2 cut(s) 251, 402
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 3 cut(s) 435, 480, 483
FokI GGATG 1 cut(s) 493
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 3 cut(s) 435, 480, 483
FspBI CTAG 2 cut(s) 410, 426
GluI GCNGC 3 cut(s) 435, 480, 483
HapII CCGG 1 cut(s) 362
Hin1II CATG 2 cut(s) 255, 406
HinfI GANTC 6 cut(s) 43, 204, 238, 256, 422, 471
HpaII CCGG 1 cut(s) 362
HphI GGTGA 2 cut(s) 271, 350
Hpy188I TCNGA 5 cut(s) 69, 99, 126, 274, 456
Hpy188III TCNNGA 6 cut(s) 149, 304, 323, 331, 371, 426
HpyAV CCTTC 2 cut(s) 209, 458
HpyCH4III ACNGT 3 cut(s) 196, 310, 418
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 1 cut(s) 437
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 2 cut(s) 255, 406
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 3 cut(s) 121, 126, 276
LpnPI CCDG 6 cut(s) 96, 162, 170, 317, 336, 375
Lsp1109I GCAGC 2 cut(s) 421, 466
LweI GCATC 1 cut(s) 446
MaeI CTAG 2 cut(s) 410, 426
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 364, 502
MalI GATC 3 cut(s) 123, 128, 278
MboI GATC 3 cut(s) 121, 126, 276
MboII GAAGA 5 cut(s) 164, 292, 360, 473, 480
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 3 cut(s) 38, 173, 266
MlyI GAGTC 3 cut(s) 213, 265, 480
MnlI CCTC 5 cut(s) 3, 103, 125, 270, 367
MseI TTAA 2 cut(s) 177, 245
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 73
NciI CCSGG 1 cut(s) 362
NdeI CATATG 1 cut(s) 496
NdeII GATC 3 cut(s) 121, 126, 276
NlaIII CATG 2 cut(s) 255, 406
PctI GAATGC 1 cut(s) 73
PfeI GAWTC 3 cut(s) 43, 238, 422
PkrI GCNGC 3 cut(s) 436, 481, 484
PleI GAGTC 3 cut(s) 212, 264, 479
PpsI GAGTC 3 cut(s) 212, 264, 479
Psp124BI GAGCTC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 376
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 2 cut(s) 177, 245
SatI GCNGC 3 cut(s) 435, 480, 483
Sau3AI GATC 3 cut(s) 121, 126, 276
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 3 cut(s) 213, 265, 480
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 1 cut(s) 446
SinI GGWCC 1 cut(s) 376
Sse9I AATT 3 cut(s) 38, 173, 266
SsiI CCGC 1 cut(s) 482
SspMI CTAG 2 cut(s) 410, 426
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 196, 310, 418
TaiI ACGT 1 cut(s) 446
TaqI TCGA 1 cut(s) 313
TasI AATT 3 cut(s) 38, 173, 266
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 3 cut(s) 43, 238, 422
Tru1I TTAA 2 cut(s) 177, 245
Tru9I TTAA 2 cut(s) 177, 245
TscAI CASTG 2 cut(s) 199, 289
TseI GCWGC 2 cut(s) 434, 479
TspDTI ATGAA 2 cut(s) 419, 474
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 2 cut(s) 199, 289
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 2 cut(s) 173, 266
XbaI TCTAGA 1 cut(s) 425
XspI CTAG 2 cut(s) 410, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.