Rh3BG316700

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005311
Physical Location & Seq
Reverse (-)
32447509 .. 32452389
4881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG316700.1

Sequence Viewer

Length: 534 bp
ATGGGGTTGAGAGGAGGAGGAGGAGCGGTGGCGGGGGCATTACCGAACCTGATTCGGAGCCTCCGAAAGGAAGCCCACCCGAAAACCCGAGCCCAACAATCGCTTCCGTCGCTCAGGCGGGCCTTCTCCCTGTACGATCAGATCAATCTGATCGACAACGTTCCGGATGACCAGCTTCGATTCCAGGGCTACACCGACACAGGTTTCAAAGTGAATGGGGTCGACTACGAAGGCAGCTTGCTTTGTATTGGCAATATGCTCATGTCTTGGGCTCCCAAAAAATTCTCCGATATCACTCCTGACAGCTTATCAATGTTCCAACTTATGCGGCCTATACCAGAGATTTTAATTCTTGGCTGTGGGAGATACATTGAACCCGTAGATCCTGAAGTACGACGCTTCATTCGGTCTACTGGAATGAAGTTAGAAGCCGTTGACTCGAGAAACGCCATATCAACTTACAACATACTGAATGAGGAAGGTAGGATCGTGGCTGCTGCACTTTTACCTTACGGATGCGGAGTTTCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

177

Amino Acids

19.46

Weight (kDa)

8.47

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 62 - 169 6.9e-34 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 26
AccI GTMKAC 2 cut(s) 222, 410
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 5 cut(s) 26, 32, 118, 328, 519
AclI AACGTT 1 cut(s) 159
AclWI GGATC 2 cut(s) 377, 494
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 2 cut(s) 134, 393
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 2 cut(s) 208, 374
AjnI CCWGG 1 cut(s) 183
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 3 cut(s) 175, 237, 306
AluI AGCT 3 cut(s) 175, 237, 306
AlwI GGATC 2 cut(s) 377, 494
Ama87I CYCGRG 2 cut(s) 87, 439
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 2 cut(s) 120, 329
ApeKI GCWGC 3 cut(s) 234, 494, 497
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
AspS9I GGNCC 1 cut(s) 120
AvaI CYCGRG 2 cut(s) 87, 439
BanII GRGCYC 2 cut(s) 94, 274
BbvI GCAGC 3 cut(s) 246, 481, 484
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 1 cut(s) 185
BisI GCNGC 4 cut(s) 235, 329, 495, 498
BlsI GCNGC 4 cut(s) 236, 330, 496, 499
Bme1390I CCNGG 1 cut(s) 185
BmeT110I CYCGRG 2 cut(s) 87, 439
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 2 cut(s) 59, 273
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 418
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 172, 521
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 4 cut(s) 27, 30, 33, 36
BseXI GCAGC 3 cut(s) 246, 481, 484
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 2 cut(s) 122, 331
BsiHKCI CYCGRG 2 cut(s) 87, 439
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 67
BsnI GGCC 2 cut(s) 122, 331
BsoBI CYCGRG 2 cut(s) 87, 439
Bsp1286I GDGCHC 2 cut(s) 94, 274
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 136, 141, 150, 382, 486
BspACI CCGC 5 cut(s) 26, 32, 118, 328, 519
BspANI GGCC 2 cut(s) 122, 331
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 163
BspHI TCATGA 1 cut(s) 530
BspLI GGNNCC 2 cut(s) 59, 273
BspPI GGATC 2 cut(s) 377, 494
BsrBI CCGCTC 1 cut(s) 26
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 5 cut(s) 136, 141, 150, 382, 486
Bst2UI CCWGG 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 120, 239
BstDEI CTNAG 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 172, 521
BstKTI GATC 5 cut(s) 139, 144, 153, 385, 489
BstMBI GATC 5 cut(s) 136, 141, 150, 382, 486
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 246, 481, 484
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 2 cut(s) 122, 331
BtsCI GGATG 2 cut(s) 172, 521
Cac8I GCNNGC 2 cut(s) 120, 239
CciI TCATGA 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 120
CseI GACGC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 133, 392
CviAII CATG 2 cut(s) 262, 531
CviQI GTAC 2 cut(s) 133, 392
DdeI CTNAG 1 cut(s) 113
DpnI GATC 5 cut(s) 138, 143, 152, 384, 488
DpnII GATC 5 cut(s) 136, 141, 150, 382, 486
Eco24I GRGCYC 2 cut(s) 94, 274
Eco32I GATATC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 2 cut(s) 87, 439
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 183
EcoRV GATATC 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 94, 274
FaeI CATG 2 cut(s) 265, 534
FaiI YATR 7 cut(s) 257, 263, 326, 335, 452, 467, 532
FatI CATG 2 cut(s) 261, 530
FauI CCCGC 2 cut(s) 25, 111
FblI GTMKAC 2 cut(s) 222, 410
Fnu4HI GCNGC 4 cut(s) 235, 329, 495, 498
FokI GGATG 2 cut(s) 179, 528
FriOI GRGCYC 2 cut(s) 94, 274
Fsp4HI GCNGC 4 cut(s) 235, 329, 495, 498
GluI GCNGC 4 cut(s) 235, 329, 495, 498
HaeIII GGCC 2 cut(s) 122, 331
HapII CCGG 1 cut(s) 164
HgaI GACGC 1 cut(s) 405
Hin1II CATG 2 cut(s) 265, 534
HincII GTYRAC 2 cut(s) 223, 436
HindII GTYRAC 2 cut(s) 223, 436
HinfI GANTC 3 cut(s) 52, 180, 437
HpaII CCGG 1 cut(s) 164
Hpy166II GTNNAC 3 cut(s) 223, 411, 436
Hpy188I TCNGA 5 cut(s) 57, 65, 141, 150, 289
Hpy188III TCNNGA 5 cut(s) 164, 299, 386, 441, 531
Hpy8I GTNNAC 3 cut(s) 223, 411, 436
Hpy99I CGWCG 2 cut(s) 112, 399
HpyAV CCTTC 3 cut(s) 133, 224, 473
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 1 cut(s) 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 2 cut(s) 265, 534
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 5 cut(s) 136, 141, 150, 382, 486
LmnI GCTCC 3 cut(s) 23, 57, 277
Lsp1109I GCAGC 3 cut(s) 246, 481, 484
LweI GCATC 1 cut(s) 506
MaeII ACGT 1 cut(s) 159
MalI GATC 5 cut(s) 138, 143, 152, 384, 488
MbiI CCGCTC 1 cut(s) 26
MboI GATC 5 cut(s) 136, 141, 150, 382, 486
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 94, 274
MluCI AATT 2 cut(s) 281, 348
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 6 cut(s) 5, 8, 11, 14, 71, 469
MroI TCCGGA 1 cut(s) 163
MseI TTAA 1 cut(s) 347
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 109
NdeII GATC 5 cut(s) 136, 141, 150, 382, 486
NlaIII CATG 2 cut(s) 265, 534
NlaIV GGNNCC 2 cut(s) 59, 273
PaeR7I CTCGAG 1 cut(s) 439
PagI TCATGA 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PfeI GAWTC 2 cut(s) 52, 180
PkrI GCNGC 4 cut(s) 236, 330, 496, 499
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
Psp1406I AACGTT 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 59, 273
PspPI GGNCC 1 cut(s) 120
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 2 cut(s) 134, 393
RsaNI GTAC 2 cut(s) 133, 392
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 4 cut(s) 235, 329, 495, 498
Sau3AI GATC 5 cut(s) 136, 141, 150, 382, 486
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 2 cut(s) 94, 274
SetI ASST 8 cut(s) 51, 162, 177, 205, 239, 308, 484, 511
SfaNI GCATC 1 cut(s) 506
Sfr274I CTCGAG 1 cut(s) 439
SlaI CTCGAG 1 cut(s) 439
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 2 cut(s) 281, 348
SsiI CCGC 5 cut(s) 26, 32, 118, 328, 519
StyD4I CCNGG 1 cut(s) 183
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 153, 178, 222, 440
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 281, 348
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 2 cut(s) 52, 180
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TseI GCWGC 3 cut(s) 234, 494, 497
TspDTI ATGAA 2 cut(s) 391, 434
TspGWI ACGGA 2 cut(s) 96, 528
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 439
XmiI GTMKAC 2 cut(s) 222, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.