Rorug03G0232900

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005311
Physical Location & Seq
Forward (+)
21482308 .. 21485715
3408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0232900.1

Sequence Viewer

Length: 1224 bp
ATGGAGAGCACAGCAGTGTGGCAAGGGGCAACACTCTGTGGGATTCTCACATGGATTCTAATGGCTTCTTATCTCAATCTGACCCCAAAGCTTCGATCTTTGCTGCAACCGTGGGTGGCCCACCATGTCATCACAGGCACGCCCCTCATCCTCCAGATCCAGAATTACCAGCATGTGTTCTTGGATGCTTTCTTCTCTGCTTTGTCCTGTGTTGTCTCTGTGCCCTTTTACACTGCTTTTCTTCCTTTGCTTTTCTGGAGTGGGCATGGCAAATTGGCAAGGCAAATGACCCTGTTGATGGCTTTCTGTGATTACTTGGGGAACTGCATAAAGGATGTGGTATCAGCTCCTAGACCGAGTTGCCCTCCTGTTAGGAGAATAACAGCTACAAGTGATGAGGAGGATAATGCATTGGAATATGGATTGCCATCTTCTCACACTCTAAACACAGTTTGCTTGTCTGGATACCTTTTGCATTACATCCTATCTTATGCTCAGAATGAAGATGTGTATGTGAAATTTGCTGGATTTGCTCTTGTTTGCTTGCTTGTGGGTCTCATTGGTTTGGGAAGAGTTTATCTCGGCATGCACAGTCCTGTTGATATTATTGGTGGGCTTGCTGTTGGTTTGCTGATCCTTTCCTTCTGGCTTACTGTTCACGAATATGTTGACAGTTTTGTAGTCTCAGGACAAAATGTTACAAGCTTTTGGGCCGCCCTAAGCTTCATATTGCTCTTCGCTTATCCAACTCCGGAGTTTCCAACTCCAAGTTTTGAGTACCACACAGCGTTCAATGGTGTTGCATTAGGCATTGTATCTGGGGTCCAGCAAACATACCACCAGTTTCACCATGAAGCTGTTCCACGTATATTTACATCACAATTAACAATCCCTTGCTTTATGGGAAGAATGCTGGTTGGGATACCAACAATACTTATTGTCAAGTTCTGTAGTAAAGCTCTTGCTAAATGGATTCTTCCTGTGGTAGCCAATACCTTGGGCATCCCAATAAAATCAACTACATACATTCCTATGCTAAATGGATTAAGCAATGGGAAAAAATCAGATGGGTCGAAGCAGTCAGGTTATCTCCAGAAGCTGTACCTTTTCTCCAGCCAAGGCTCATTTGATGTCGATACTGGTATAAGATTCATTCAATATGCTGGCCTTGCATGGTCAGTGGTAGATCTTGTTCCTTCTCTTTTCTCTCAGCTAAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

407

Amino Acids

44.85

Weight (kDa)

7.16

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 96 - 222 5.8e-21 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 751
AciI CCGC 1 cut(s) 714
AclWI GGATC 2 cut(s) 151, 628
AcsI RAATTY 1 cut(s) 518
AdeI CACNNNGTG 1 cut(s) 38
AfaI GTAC 2 cut(s) 779, 1103
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 2 cut(s) 793, 1157
AleI CACNNNNGTG 1 cut(s) 14
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 220, 560, 688
AlwI GGATC 2 cut(s) 151, 628
AlwNI CAGNNNCTG 1 cut(s) 1099
Aor13HI TCCGGA 1 cut(s) 751
AoxI GGCC 3 cut(s) 117, 711, 1165
ApeKI GCWGC 1 cut(s) 103
ApoI RAATTY 1 cut(s) 518
Asp700I GAANNNNTTC 1 cut(s) 858
AspS9I GGNCC 3 cut(s) 118, 711, 823
AsuHPI GGTGA 1 cut(s) 839
AvaII GGWCC 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 90
BccI CCATC 3 cut(s) 292, 436, 1061
BciVI GTATCC 2 cut(s) 458, 915
BcoDI GTCTC 3 cut(s) 220, 560, 688
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 949
BfuI GTATCC 2 cut(s) 458, 915
BglII AGATCT 1 cut(s) 1186
BisI GCNGC 2 cut(s) 104, 714
BlpI GCTNAGC 1 cut(s) 1214
BlsI GCNGC 2 cut(s) 105, 715
Bme18I GGWCC 1 cut(s) 823
BmgT120I GGNCC 3 cut(s) 118, 711, 823
BmiI GGNNCC 1 cut(s) 824
BmsI GCATC 2 cut(s) 175, 1011
BplI GAGNNNNNCTC 4 cut(s) 349, 381, 564, 596
BpmI CTGGAG 4 cut(s) 137, 277, 1076, 1096
Bpu10I CCTNAGC 1 cut(s) 719
Bpu1102I GCTNAGC 1 cut(s) 1214
BsaAI YACGTR 1 cut(s) 866
BsaBI GATNNNNATC 1 cut(s) 427
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 110, 996, 1117
BsaWI WCCGGW 1 cut(s) 751
BsaXI ACNNNNNCTCC 2 cut(s) 1094, 1124
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 2 cut(s) 841, 1144
Bse3DI GCAATG 1 cut(s) 1057
Bse8I GATNNNNATC 1 cut(s) 427
BseAI TCCGGA 1 cut(s) 751
BseDI CCNNGG 3 cut(s) 110, 996, 1117
BseGI GGATG 5 cut(s) 147, 190, 340, 480, 1002
BseJI GATNNNNATC 1 cut(s) 427
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 1 cut(s) 1057
BseMII CTCAG 3 cut(s) 509, 699, 1223
BseNI ACTGG 2 cut(s) 841, 1144
BseRI GAGGAG 1 cut(s) 413
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 1 cut(s) 90
BshFI GGCC 3 cut(s) 119, 713, 1167
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 1 cut(s) 752
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 3 cut(s) 220, 560, 688
BsmI GAATGC 1 cut(s) 915
BsnI GGCC 3 cut(s) 119, 713, 1167
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 11, 225
Bsp13I TCCGGA 1 cut(s) 751
Bsp143I GATC 4 cut(s) 95, 156, 633, 1186
Bsp1720I GCTNAGC 1 cut(s) 1214
BspACI CCGC 1 cut(s) 714
BspANI GGCC 3 cut(s) 119, 713, 1167
BspCNI CTCAG 3 cut(s) 508, 698, 1222
BspEI TCCGGA 1 cut(s) 751
BspLI GGNNCC 1 cut(s) 824
BspPI GGATC 2 cut(s) 151, 628
BspQI GCTCTTC 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 1057
BsrI ACTGG 2 cut(s) 841, 1144
BssECI CCNNGG 3 cut(s) 110, 996, 1117
BssMI GATC 4 cut(s) 95, 156, 633, 1186
BssT1I CCWWGG 2 cut(s) 996, 1117
Bst4CI ACNGT 5 cut(s) 111, 451, 593, 655, 674
Bst6I CTCTTC 2 cut(s) 565, 740
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 5 cut(s) 140, 545, 587, 618, 1165
BstDEI CTNAG 5 cut(s) 495, 685, 719, 1209, 1214
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 5 cut(s) 147, 190, 340, 480, 1002
BstKTI GATC 4 cut(s) 98, 159, 636, 1189
BstMAI GTCTC 3 cut(s) 220, 560, 688
BstMBI GATC 4 cut(s) 95, 156, 633, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 530, 1169
BstNSI RCATGY 2 cut(s) 176, 589
BstSFI CTRYAG 1 cut(s) 949
BstSLI GKGCMC 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 90
BstX2I RGATCY 2 cut(s) 156, 1186
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 2 cut(s) 156, 1186
BsuI GTATCC 2 cut(s) 458, 915
BsuRI GGCC 3 cut(s) 119, 713, 1167
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 5 cut(s) 147, 190, 340, 480, 1002
BtsI GCAGTG 2 cut(s) 21, 231
BtsIMutI CAGTG 3 cut(s) 21, 231, 1185
Cac8I GCNNGC 5 cut(s) 140, 545, 587, 618, 1165
CaiI CAGNNNCTG 1 cut(s) 1099
Cfr13I GGNCC 3 cut(s) 118, 711, 823
Csp6I GTAC 2 cut(s) 778, 1102
CviAII CATG 7 cut(s) 51, 125, 173, 266, 586, 851, 1173
CviQI GTAC 2 cut(s) 778, 1102
DdeI CTNAG 5 cut(s) 495, 685, 719, 1209, 1214
DpnI GATC 4 cut(s) 97, 158, 635, 1188
DpnII GATC 4 cut(s) 95, 156, 633, 1186
DraIII CACNNNGTG 1 cut(s) 38
Eam1104I CTCTTC 2 cut(s) 565, 740
EarI CTCTTC 2 cut(s) 565, 740
Eco130I CCWWGG 2 cut(s) 996, 1117
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 823
EcoT14I CCWWGG 2 cut(s) 996, 1117
EcoT22I ATGCAT 1 cut(s) 412
ErhI CCWWGG 2 cut(s) 996, 1117
FaeI CATG 7 cut(s) 54, 128, 176, 269, 589, 854, 1176
FatI CATG 7 cut(s) 50, 124, 172, 265, 585, 850, 1172
Fnu4HI GCNGC 2 cut(s) 104, 714
FokI GGATG 5 cut(s) 134, 197, 347, 467, 989
Fsp4HI GCNGC 2 cut(s) 104, 714
FspBI CTAG 1 cut(s) 351
GluI GCNGC 2 cut(s) 104, 714
GsuI CTGGAG 4 cut(s) 137, 277, 1076, 1096
HaeIII GGCC 3 cut(s) 119, 713, 1167
HapII CCGG 1 cut(s) 752
Hin1II CATG 7 cut(s) 54, 128, 176, 269, 589, 854, 1176
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 4 cut(s) 89, 703, 721, 1216
HinfI GANTC 4 cut(s) 43, 55, 973, 1149
HpaII CCGG 1 cut(s) 752
HphI GGTGA 1 cut(s) 839
Hpy166II GTNNAC 2 cut(s) 658, 670
Hpy188I TCNGA 3 cut(s) 81, 498, 1066
Hpy188III TCNNGA 8 cut(s) 154, 160, 256, 462, 659, 687, 752, 1093
Hpy8I GTNNAC 2 cut(s) 658, 670
HpyAV CCTTC 2 cut(s) 652, 1206
HpyCH4III ACNGT 5 cut(s) 111, 451, 593, 655, 674
HpyCH4IV ACGT 1 cut(s) 865
HpyCH4V TGCA 7 cut(s) 106, 327, 410, 475, 589, 803, 1172
HpyF10VI GCNNNNNNNGC 2 cut(s) 530, 1169
HpyF3I CTNAG 5 cut(s) 495, 685, 719, 1209, 1214
HpySE526I ACGT 1 cut(s) 865
Hsp92II CATG 7 cut(s) 54, 128, 176, 269, 589, 854, 1176
Kpn2I TCCGGA 1 cut(s) 751
Kzo9I GATC 4 cut(s) 95, 156, 633, 1186
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 1 cut(s) 90
LweI GCATC 2 cut(s) 175, 1011
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 865
MaeIII GTNAC 1 cut(s) 697
MalI GATC 4 cut(s) 97, 158, 635, 1188
MboI GATC 4 cut(s) 95, 156, 633, 1186
MboII GAAGA 8 cut(s) 184, 233, 423, 515, 582, 727, 918, 968
MflI RGATCY 2 cut(s) 156, 1186
MhlI GDGCHC 2 cut(s) 11, 225
MluCI AATT 4 cut(s) 163, 272, 518, 881
MmeI TCCRAC 2 cut(s) 770, 785
MnlI CCTC 5 cut(s) 155, 161, 375, 391, 394
Mph1103I ATGCAT 1 cut(s) 412
MroI TCCGGA 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 858
MseI TTAA 2 cut(s) 884, 1046
MslI CAYNNNNRTG 3 cut(s) 14, 663, 1031
MspI CCGG 1 cut(s) 752
Mva1269I GAATGC 1 cut(s) 915
MwoI GCNNNNNNNGC 2 cut(s) 530, 1169
NdeII GATC 4 cut(s) 95, 156, 633, 1186
NlaIII CATG 7 cut(s) 54, 128, 176, 269, 589, 854, 1176
NlaIV GGNNCC 1 cut(s) 824
NmeAIII GCCGAG 1 cut(s) 561
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 2 cut(s) 176, 589
OliI CACNNNNGTG 1 cut(s) 14
PaeI GCATGC 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 740
PctI GAATGC 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 4 cut(s) 43, 55, 973, 1149
PkrI GCNGC 2 cut(s) 105, 715
Ppu21I YACGTR 1 cut(s) 866
PspN4I GGNNCC 1 cut(s) 824
PspPI GGNCC 3 cut(s) 118, 711, 823
PsrI GAACNNNNNNTAC 2 cut(s) 1176, 1208
PstNI CAGNNNCTG 1 cut(s) 1099
PsuI RGATCY 2 cut(s) 156, 1186
RsaI GTAC 2 cut(s) 779, 1103
RsaNI GTAC 2 cut(s) 778, 1102
RseI CAYNNNNRTG 3 cut(s) 14, 663, 1031
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 2 cut(s) 884, 1046
SatI GCNGC 2 cut(s) 104, 714
Sau3AI GATC 4 cut(s) 95, 156, 633, 1186
Sau96I GGNCC 3 cut(s) 118, 711, 823
SduI GDGCHC 2 cut(s) 11, 225
SfaNI GCATC 2 cut(s) 175, 1011
SfcI CTRYAG 1 cut(s) 949
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 3 cut(s) 14, 663, 1031
SphI GCATGC 1 cut(s) 589
Sse9I AATT 4 cut(s) 163, 272, 518, 881
SsiI CCGC 1 cut(s) 714
SspMI CTAG 1 cut(s) 351
StyI CCWWGG 2 cut(s) 996, 1117
TaaI ACNGT 5 cut(s) 111, 451, 593, 655, 674
TaiI ACGT 1 cut(s) 868
TaqI TCGA 3 cut(s) 94, 1073, 1134
TaqII GACCGA 1 cut(s) 370
TasI AATT 4 cut(s) 163, 272, 518, 881
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 4 cut(s) 43, 55, 973, 1149
Tru1I TTAA 2 cut(s) 884, 1046
Tru9I TTAA 2 cut(s) 884, 1046
TscAI CASTG 3 cut(s) 21, 238, 1185
TseI GCWGC 1 cut(s) 103
TspDTI ATGAA 4 cut(s) 516, 715, 867, 1141
TspRI CASTG 3 cut(s) 21, 238, 1185
VpaK11BI GGWCC 1 cut(s) 823
XapI RAATTY 1 cut(s) 518
XceI RCATGY 2 cut(s) 176, 589
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.