MD09G1285000.v1.1

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005311
Physical Location & Seq
Forward (+)
36288639 .. 36306620
17982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1285000.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGGGTCGAGAGCGAGAGCAGCCGGGGCATTACCAAACCTCATTCGGACCCTCCGGAAAGAAGCCCGCCCGAAACACGCAACCCAACAATCACTCCCGTCTCTCAGGCGCGCCTTCTCTCTCTACGATCAGATCAATCTCATCGACAACGTCCCCGACGACCAGCTTCGCTTTCAACGGTACACAGAGAATGGATTCACAGTGAATGGTGTGGACTATGAAGGAAGCTTGCTTTGTGTGGGAAATATGTTGATGTCTTGGGCTCCCACGAAATTCTCTGATGTCACTCCCGATAGCTTATCAATCTTCAAACTTGTGCGGCCTATACCTGGTGAAGCATTGGGGTTGAAGCTTGATAGCTTTATAGAGATTTTGATTCTCGGCTGTGGGAGAAACATTGAACCCGTAGATCCTGAACTACGACGCTACATTCGTTCTACGGGAATGAAGTTAGAAGCCGTTGACTCGAGAAACGCTATATCAACTTACAACATTCTGAACGAAGAAGGTAGGATTGTGGCTGCTGCACTCTTGCCATACGGAGCGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

184

Amino Acids

20.28

Weight (kDa)

8.6

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 59 - 178 3.7e-30 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 545
AccII CGCG 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 3 cut(s) 67, 319, 545
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 1 cut(s) 272
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 1 cut(s) 329
AgsI TTSAA 4 cut(s) 176, 310, 349, 401
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 5 cut(s) 166, 228, 297, 352, 360
AluI AGCT 5 cut(s) 166, 228, 297, 352, 360
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 466
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 3 cut(s) 20, 521, 524
ApoI RAATTY 1 cut(s) 272
ArsI GACNNNNNNTTYG 2 cut(s) 414, 446
AscI GGCGCGCC 1 cut(s) 109
Asp700I GAANNNNTTC 1 cut(s) 194
AspLEI GCGC 2 cut(s) 111, 113
AspS9I GGNCC 1 cut(s) 48
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 1 cut(s) 344
AvaI CYCGRG 1 cut(s) 466
AvaII GGWCC 1 cut(s) 48
BanII GRGCYC 1 cut(s) 265
BbvI GCAGC 3 cut(s) 32, 508, 511
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 105
BisI GCNGC 5 cut(s) 21, 320, 522, 525, 546
BlsI GCNGC 5 cut(s) 22, 321, 523, 526, 547
Bme1390I CCNGG 2 cut(s) 25, 330
Bme18I GGWCC 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 50, 264
BmrFI CCNGG 2 cut(s) 25, 330
BpuMI CCSGG 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 24
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 329
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 329
BseMII CTCAG 1 cut(s) 118
BsePI GCGCGC 1 cut(s) 109
BseXI GCAGC 3 cut(s) 32, 508, 511
BsgI GTGCAG 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 322
BsiHKCI CYCGRG 1 cut(s) 466
BsiSI CCGG 2 cut(s) 24, 55
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 105
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 265
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 3 cut(s) 127, 132, 409
BspACI CCGC 3 cut(s) 67, 319, 545
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 54
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 2 cut(s) 50, 264
BspPI GGATC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 545
BssECI CCNNGG 1 cut(s) 24
BssHII GCGCGC 1 cut(s) 109
BssMI GATC 3 cut(s) 127, 132, 409
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 180, 202
BstC8I GCNNGC 3 cut(s) 67, 111, 230
BstDEI CTNAG 1 cut(s) 104
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 2 cut(s) 111, 113
BstKTI GATC 3 cut(s) 130, 135, 412
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 3 cut(s) 127, 132, 409
BstMWI GCNNNNNNNGC 2 cut(s) 20, 26
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 23, 328
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 32, 508, 511
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BsuRI GGCC 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 3 cut(s) 67, 111, 230
CfoI GCGC 2 cut(s) 111, 113
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 1 cut(s) 432
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 1 cut(s) 181
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 1 cut(s) 104
DpnI GATC 3 cut(s) 129, 134, 411
DpnII GATC 3 cut(s) 127, 132, 409
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 105
FaiI YATR 6 cut(s) 219, 248, 326, 365, 479, 538
FaqI GGGAC 1 cut(s) 137
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 5 cut(s) 21, 320, 522, 525, 546
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 5 cut(s) 21, 320, 522, 525, 546
GlaI GCGC 2 cut(s) 110, 112
GluI GCNGC 5 cut(s) 21, 320, 522, 525, 546
HaeIII GGCC 1 cut(s) 322
HapII CCGG 2 cut(s) 24, 55
HgaI GACGC 1 cut(s) 432
HhaI GCGC 2 cut(s) 111, 113
Hin6I GCGC 2 cut(s) 109, 111
HinP1I GCGC 2 cut(s) 109, 111
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 2 cut(s) 226, 350
HinfI GANTC 3 cut(s) 195, 376, 464
HpaII CCGG 2 cut(s) 24, 55
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 183, 214, 463
Hpy188I TCNGA 4 cut(s) 48, 132, 280, 498
Hpy188III TCNNGA 5 cut(s) 9, 55, 290, 413, 468
Hpy8I GTNNAC 3 cut(s) 183, 214, 463
Hpy99I CGWCG 2 cut(s) 161, 426
HpyAV CCTTC 3 cut(s) 124, 215, 500
HpyCH4III ACNGT 2 cut(s) 180, 202
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 26
HpyF3I CTNAG 1 cut(s) 104
HpySE526I ACGT 1 cut(s) 150
HspAI GCGC 2 cut(s) 109, 111
Kpn2I TCCGGA 1 cut(s) 54
Kzo9I GATC 3 cut(s) 127, 132, 409
LmnI GCTCC 2 cut(s) 268, 542
LpnPI CCDG 7 cut(s) 37, 68, 91, 176, 315, 342, 426
Lsp1109I GCAGC 3 cut(s) 32, 508, 511
MabI ACCWGGT 1 cut(s) 328
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 283
MalI GATC 3 cut(s) 129, 134, 411
MbiI CCGCTC 1 cut(s) 545
MboI GATC 3 cut(s) 127, 132, 409
MboII GAAGA 2 cut(s) 298, 515
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 1 cut(s) 272
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 2 cut(s) 50, 62
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 194
MseI TTAA 1 cut(s) 550
MspI CCGG 2 cut(s) 24, 55
MspR9I CCNGG 2 cut(s) 25, 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 20, 26
NciI CCSGG 1 cut(s) 25
NdeII GATC 3 cut(s) 127, 132, 409
NlaIV GGNNCC 2 cut(s) 50, 264
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 466
PalAI GGCGCGCC 1 cut(s) 109
PauI GCGCGC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 430
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 195, 376
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 5 cut(s) 22, 321, 523, 526, 547
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 50, 264
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 409
PsyI GACNNNGTC 1 cut(s) 149
PteI GCGCGC 1 cut(s) 109
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SaqAI TTAA 1 cut(s) 550
SatI GCNGC 5 cut(s) 21, 320, 522, 525, 546
Sau3AI GATC 3 cut(s) 127, 132, 409
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 25, 330
SduI GDGCHC 1 cut(s) 265
SetI ASST 9 cut(s) 42, 153, 168, 230, 299, 331, 354, 362, 511
SexAI ACCWGGT 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 466
SgsI GGCGCGCC 1 cut(s) 109
SinI GGWCC 1 cut(s) 48
SlaI CTCGAG 1 cut(s) 466
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 1 cut(s) 272
SsiI CCGC 3 cut(s) 67, 319, 545
StyD4I CCNGG 2 cut(s) 23, 328
TaaI ACNGT 2 cut(s) 180, 202
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 8, 144, 467
TasI AATT 1 cut(s) 272
TauI GCSGC 2 cut(s) 322, 548
TfiI GAWTC 2 cut(s) 195, 376
Tru1I TTAA 1 cut(s) 550
Tru9I TTAA 1 cut(s) 550
TscAI CASTG 1 cut(s) 207
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 3 cut(s) 20, 521, 524
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 234, 461
TspRI CASTG 1 cut(s) 207
Tth111I GACNNNGTC 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 48
XapI RAATTY 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.