Rh3DG312700

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005311
Physical Location & Seq
Reverse (-)
33597245 .. 33602298
5054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG312700.1

Sequence Viewer

Length: 477 bp
ATGGGGTTGAGAGGAGGAGGAGGAGTAGTGGCGGGGGCATTACCGAACCTGATTCGGAGCCTCCGAAAGGAAGCCCACCCGAAGACCCGAGCCCAACAATCACTACCGTCTCTCAGGCGGGCCTTCTCCCTGTACGATCAGATCAATCTGATCGACAACGTTCCGGATGACCAGCTTCGATTTCAAGGGTACACCGACACAGGTTTCAAAGTGAATGGGGTCGACTACGAAGGCAGCTTGCTTTGTATTGGCAACATGCTCATGTCTTGGGCTCCCAAAAAATTCTCCGATATCACTCCTGACAGCTTATCAATGTTCCAACTTATGCGGCCTATACCAGAGATTTTAATTCTTGGCTGTGGGAGATACATTGAACCCGTAGATCCTGAAGTACGACGCTTCATTCGGTCTACTGGAATGAAGTTAGAAGCCGTTGACTCGGTATATTTCATCATTGCTTCTATTTTCTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

158

Amino Acids

17.67

Weight (kDa)

8.62

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 62 - 147 5.5e-21 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 222, 410
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 3 cut(s) 32, 118, 328
AclI AACGTT 1 cut(s) 159
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 134, 191, 393
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 3 cut(s) 185, 208, 374
AluBI AGCT 3 cut(s) 175, 237, 306
AluI AGCT 3 cut(s) 175, 237, 306
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 87
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 2 cut(s) 120, 329
ApeKI GCWGC 1 cut(s) 234
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
AspS9I GGNCC 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 87
BanII GRGCYC 2 cut(s) 94, 274
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 246
BceAI ACGGC 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 114
BisI GCNGC 2 cut(s) 235, 329
BlsI GCNGC 2 cut(s) 236, 330
BmeT110I CYCGRG 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 2 cut(s) 59, 273
BpiI GAAGAC 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 4 cut(s) 9, 12, 39, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 453
BseAI TCCGGA 1 cut(s) 163
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 4 cut(s) 27, 30, 33, 36
BseXI GCAGC 1 cut(s) 246
BshFI GGCC 2 cut(s) 122, 331
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 114
BsmBI CGTCTC 1 cut(s) 114
BsnI GGCC 2 cut(s) 122, 331
BsoBI CYCGRG 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 94, 274
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 4 cut(s) 136, 141, 150, 382
BspACI CCGC 3 cut(s) 32, 118, 328
BspANI GGCC 2 cut(s) 122, 331
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 59, 273
BspPI GGATC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 1 cut(s) 418
BssMI GATC 4 cut(s) 136, 141, 150, 382
Bst4CI ACNGT 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 120, 239
BstDEI CTNAG 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 4 cut(s) 139, 144, 153, 385
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 4 cut(s) 136, 141, 150, 382
BstNSI RCATGY 1 cut(s) 259
BstV1I GCAGC 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 2 cut(s) 122, 331
BtsCI GGATG 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 120, 239
Cfr13I GGNCC 1 cut(s) 120
CseI GACGC 1 cut(s) 405
Csp6I GTAC 3 cut(s) 133, 190, 392
CviAII CATG 2 cut(s) 256, 262
CviQI GTAC 3 cut(s) 133, 190, 392
DdeI CTNAG 1 cut(s) 113
DpnI GATC 4 cut(s) 138, 143, 152, 384
DpnII GATC 4 cut(s) 136, 141, 150, 382
Eco24I GRGCYC 2 cut(s) 94, 274
Eco32I GATATC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 87
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoRV GATATC 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 94, 274
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 2 cut(s) 259, 265
FaiI YATR 5 cut(s) 257, 263, 326, 335, 445
FatI CATG 2 cut(s) 255, 261
FauI CCCGC 2 cut(s) 25, 111
FblI GTMKAC 2 cut(s) 222, 410
Fnu4HI GCNGC 2 cut(s) 235, 329
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 94, 274
Fsp4HI GCNGC 2 cut(s) 235, 329
GluI GCNGC 2 cut(s) 235, 329
HaeIII GGCC 2 cut(s) 122, 331
HapII CCGG 1 cut(s) 164
HgaI GACGC 1 cut(s) 405
Hin1II CATG 2 cut(s) 259, 265
HincII GTYRAC 2 cut(s) 223, 436
HindII GTYRAC 2 cut(s) 223, 436
HinfI GANTC 2 cut(s) 52, 437
HpaII CCGG 1 cut(s) 164
Hpy166II GTNNAC 4 cut(s) 192, 223, 411, 436
Hpy188I TCNGA 5 cut(s) 57, 65, 141, 150, 289
Hpy188III TCNNGA 3 cut(s) 164, 299, 386
Hpy8I GTNNAC 4 cut(s) 192, 223, 411, 436
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 2 cut(s) 133, 224
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4IV ACGT 1 cut(s) 159
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 2 cut(s) 259, 265
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 4 cut(s) 136, 141, 150, 382
LmnI GCTCC 2 cut(s) 57, 277
Lsp1109I GCAGC 1 cut(s) 246
MaeII ACGT 1 cut(s) 159
MalI GATC 4 cut(s) 138, 143, 152, 384
MboI GATC 4 cut(s) 136, 141, 150, 382
MboII GAAGA 1 cut(s) 94
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 94, 274
MluCI AATT 2 cut(s) 281, 348
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 5, 8, 11, 14, 71
MroI TCCGGA 1 cut(s) 163
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 260
MspI CCGG 1 cut(s) 164
NdeII GATC 4 cut(s) 136, 141, 150, 382
NlaIII CATG 2 cut(s) 259, 265
NlaIV GGNNCC 2 cut(s) 59, 273
NspI RCATGY 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 2 cut(s) 236, 330
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
Psp1406I AACGTT 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 59, 273
PspPI GGNCC 1 cut(s) 120
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 3 cut(s) 134, 191, 393
RsaNI GTAC 3 cut(s) 133, 190, 392
RseI CAYNNNNRTG 1 cut(s) 260
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 2 cut(s) 235, 329
Sau3AI GATC 4 cut(s) 136, 141, 150, 382
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 2 cut(s) 94, 274
SetI ASST 6 cut(s) 51, 162, 177, 205, 239, 308
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 2 cut(s) 281, 348
SsiI CCGC 3 cut(s) 32, 118, 328
TaaI ACNGT 1 cut(s) 108
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 153, 178, 222
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 281, 348
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TseI GCWGC 1 cut(s) 234
TspDTI ATGAA 3 cut(s) 391, 434, 439
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 222, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.