Rh3AG280900

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005311
Physical Location & Seq
Reverse (-)
32760051 .. 32765344
5294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG280900.1

Sequence Viewer

Length: 474 bp
ATGGGGTTGAGAGGAGGAGGAGCAGTGGCGGGGCAATTACCGAACCTGATTCGGAGCCTCCGAAAGGAAGCCCACCCGAAGACCCGAGCCCAACAATCACTACCGTCTCTCAGGCGGGCCTTCTCCCTGTACGATCAGATCAATCTCATCGACAACGTTCCGGATGACCAGCTTCGATTTCAAGGGTACACCGACACAGGTTTCAAAGTGAATGGGGTCGACTACGAAGGCAGCTTGCTTTGTATTGGCAATATGCTCATGTCTTGGGCTCCCAAAAAATTCTCCGATATCACTCCTGACAGCTTATCAATGTTCCAACTTATGCGGCCTATACCAGAGATTTTAATTCTTGGCTGTGGGAGATTCATTGAACCCGTAGATCCTGAAGTACGACGCTTCATTCGGTCTACTGGAATGAAGTTAGAAGCTGTTGACTCGGTATATTTCATCATTGCTTCTATTTTCTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

157

Amino Acids

17.63

Weight (kDa)

8.64

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 61 - 146 3.9e-20 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 219, 407
AccIII TCCGGA 1 cut(s) 160
AciI CCGC 3 cut(s) 29, 115, 325
AclI AACGTT 1 cut(s) 156
AclWI GGATC 1 cut(s) 374
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 3 cut(s) 131, 188, 390
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 3 cut(s) 182, 205, 371
AluBI AGCT 4 cut(s) 172, 234, 303, 428
AluI AGCT 4 cut(s) 172, 234, 303, 428
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 84
Aor13HI TCCGGA 1 cut(s) 160
AoxI GGCC 2 cut(s) 117, 326
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 1 cut(s) 278
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AspS9I GGNCC 1 cut(s) 117
AvaI CYCGRG 1 cut(s) 84
BanII GRGCYC 2 cut(s) 91, 271
BbsI GAAGAC 1 cut(s) 86
BbvI GCAGC 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 111
BisI GCNGC 2 cut(s) 232, 326
BlsI GCNGC 2 cut(s) 233, 327
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 2 cut(s) 56, 270
BpiI GAAGAC 1 cut(s) 86
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 1 cut(s) 450
BseAI TCCGGA 1 cut(s) 160
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 3 cut(s) 27, 30, 33
BseXI GCAGC 1 cut(s) 243
BshFI GGCC 2 cut(s) 119, 328
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 111
BsmBI CGTCTC 1 cut(s) 111
BsnI GGCC 2 cut(s) 119, 328
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 91, 271
Bsp13I TCCGGA 1 cut(s) 160
Bsp143I GATC 3 cut(s) 133, 138, 379
BspACI CCGC 3 cut(s) 29, 115, 325
BspANI GGCC 2 cut(s) 119, 328
BspCNI CTCAG 1 cut(s) 123
BspEI TCCGGA 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 56, 270
BspPI GGATC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 1 cut(s) 415
BssMI GATC 3 cut(s) 133, 138, 379
Bst4CI ACNGT 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 117, 236
BstDEI CTNAG 1 cut(s) 110
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 3 cut(s) 136, 141, 382
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 3 cut(s) 133, 138, 379
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 2 cut(s) 119, 328
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 117, 236
Cfr13I GGNCC 1 cut(s) 117
CseI GACGC 1 cut(s) 402
Csp6I GTAC 3 cut(s) 130, 187, 389
CviAII CATG 1 cut(s) 259
CviQI GTAC 3 cut(s) 130, 187, 389
DdeI CTNAG 1 cut(s) 110
DpnI GATC 3 cut(s) 135, 140, 381
DpnII GATC 3 cut(s) 133, 138, 379
Eco24I GRGCYC 2 cut(s) 91, 271
Eco32I GATATC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoRV GATATC 1 cut(s) 289
EcoT38I GRGCYC 2 cut(s) 91, 271
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 1 cut(s) 262
FaiI YATR 5 cut(s) 254, 260, 323, 332, 442
FatI CATG 1 cut(s) 258
FauI CCCGC 2 cut(s) 22, 108
FblI GTMKAC 2 cut(s) 219, 407
Fnu4HI GCNGC 2 cut(s) 232, 326
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 2 cut(s) 91, 271
Fsp4HI GCNGC 2 cut(s) 232, 326
GluI GCNGC 2 cut(s) 232, 326
HaeIII GGCC 2 cut(s) 119, 328
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 402
Hin1II CATG 1 cut(s) 262
HincII GTYRAC 2 cut(s) 220, 433
HindII GTYRAC 2 cut(s) 220, 433
HinfI GANTC 3 cut(s) 49, 363, 434
HpaII CCGG 1 cut(s) 161
Hpy166II GTNNAC 4 cut(s) 189, 220, 408, 433
Hpy188I TCNGA 4 cut(s) 54, 62, 138, 286
Hpy188III TCNNGA 3 cut(s) 161, 296, 383
Hpy8I GTNNAC 4 cut(s) 189, 220, 408, 433
Hpy99I CGWCG 1 cut(s) 396
HpyAV CCTTC 2 cut(s) 130, 221
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4IV ACGT 1 cut(s) 156
HpyF3I CTNAG 1 cut(s) 110
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 1 cut(s) 262
Kpn2I TCCGGA 1 cut(s) 160
Kzo9I GATC 3 cut(s) 133, 138, 379
LmnI GCTCC 3 cut(s) 20, 54, 274
Lsp1109I GCAGC 1 cut(s) 243
MaeII ACGT 1 cut(s) 156
MalI GATC 3 cut(s) 135, 140, 381
MboI GATC 3 cut(s) 133, 138, 379
MboII GAAGA 1 cut(s) 91
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 91, 271
MluCI AATT 3 cut(s) 35, 278, 345
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 4 cut(s) 5, 8, 11, 68
MroI TCCGGA 1 cut(s) 160
MseI TTAA 1 cut(s) 344
MspI CCGG 1 cut(s) 161
NdeII GATC 3 cut(s) 133, 138, 379
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 2 cut(s) 56, 270
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PfeI GAWTC 2 cut(s) 49, 363
PkrI GCNGC 2 cut(s) 233, 327
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Psp1406I AACGTT 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 56, 270
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 1 cut(s) 379
RsaI GTAC 3 cut(s) 131, 188, 390
RsaNI GTAC 3 cut(s) 130, 187, 389
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 2 cut(s) 232, 326
Sau3AI GATC 3 cut(s) 133, 138, 379
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 1 cut(s) 428
SduI GDGCHC 2 cut(s) 91, 271
SetI ASST 7 cut(s) 48, 159, 174, 202, 236, 305, 430
Sse9I AATT 3 cut(s) 35, 278, 345
SsiI CCGC 3 cut(s) 29, 115, 325
TaaI ACNGT 1 cut(s) 105
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 150, 175, 219
TaqII GACCGA 1 cut(s) 393
TasI AATT 3 cut(s) 35, 278, 345
TauI GCSGC 1 cut(s) 328
TfiI GAWTC 2 cut(s) 49, 363
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 1 cut(s) 30
TseI GCWGC 1 cut(s) 231
TspDTI ATGAA 4 cut(s) 355, 388, 431, 436
TspRI CASTG 1 cut(s) 30
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 1 cut(s) 278
XmiI GTMKAC 2 cut(s) 219, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.