FvH4_1g27010

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005311
Physical Location & Seq
Reverse (-)
18789108 .. 18793639
4532 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27010.t1

Sequence Viewer

Length: 534 bp
ATGGGGCTGAGAGGAGGAAGTGGTGCGGTGGCGGGGGCATTACCGAACCTAATTCGGAGCCTCCGGAATGAATCCCACCCGAAGACCCGAGCCCAGCAATCGCTTCCGTCGTTGAGACGCGCATTCTCTCTGTACGATCAGATCAATTTAATCGACAACGTTCCGGATGACCAGCTCCGATTTCAAGGATACACTGACACGGGTTTCAAGGTGAATGGGGTCGACTACGAGGGAAGCTTGCTTTGTATCGGCAACATGCTCATGTCTTGGGCTCCCAAAAAGTTCTCTGACATCACTCCTGATAGCTTGTCAATGTTCCAACTTATGCGGCCTATACCAGAGATTTTGATTCTTGGCTGTGGGAGATACATTGAGCCTGTGGATCCTGAAGTGCGACTCTTTGTTCGGTCTACTGGAATGAAGTTAGAAGCCGTTGATTCGAGAAATGCCATATCAACTTACAACATACTGAATGAGGAAGGTAGGATTGTGGCTGCTGCGCTTTTACCTTACGGATGTGGAGTTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

178

Amino Acids

19.44

Weight (kDa)

6.58

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 62 - 169 8.2e-33 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 222, 410
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 2 cut(s) 63, 163
AciI CCGC 3 cut(s) 26, 32, 328
AclI AACGTT 1 cut(s) 159
AclWI GGATC 2 cut(s) 377, 390
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 1 cut(s) 134
AgsI TTSAA 2 cut(s) 185, 208
AluBI AGCT 3 cut(s) 175, 237, 306
AluI AGCT 3 cut(s) 175, 237, 306
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 2 cut(s) 377, 390
Ama87I CYCGRG 1 cut(s) 87
Aor13HI TCCGGA 2 cut(s) 63, 163
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 494, 497
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
AspLEI GCGC 2 cut(s) 122, 502
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 87
BamHI GGATCC 1 cut(s) 382
BanII GRGCYC 2 cut(s) 94, 274
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 481, 484
BceAI ACGGC 1 cut(s) 416
BciVI GTATCC 1 cut(s) 182
BcoDI GTCTC 1 cut(s) 109
BfuI GTATCC 1 cut(s) 182
BisI GCNGC 3 cut(s) 329, 495, 498
BlsI GCNGC 3 cut(s) 330, 496, 499
BmeT110I CYCGRG 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 59, 273, 384
BpiI GAAGAC 1 cut(s) 89
BsaWI WCCGGW 2 cut(s) 63, 163
Bse1I ACTGG 1 cut(s) 418
BseAI TCCGGA 2 cut(s) 63, 163
BseGI GGATG 2 cut(s) 172, 521
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 481, 484
BseYI CCCAGC 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 2 cut(s) 64, 164
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 1 cut(s) 331
BsoBI CYCGRG 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 94, 274
Bsp13I TCCGGA 2 cut(s) 63, 163
Bsp143I GATC 3 cut(s) 136, 141, 382
BspACI CCGC 3 cut(s) 26, 32, 328
BspANI GGCC 1 cut(s) 331
BspEI TCCGGA 2 cut(s) 63, 163
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 530
BspLI GGNNCC 3 cut(s) 59, 273, 384
BspPI GGATC 2 cut(s) 377, 390
BsrI ACTGG 1 cut(s) 418
BssMI GATC 3 cut(s) 136, 141, 382
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 2 cut(s) 172, 521
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 2 cut(s) 122, 502
BstKTI GATC 3 cut(s) 139, 144, 385
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 136, 141, 382
BstNSI RCATGY 1 cut(s) 259
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 481, 484
BstV2I GAAGAC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 1 cut(s) 331
BtsCI GGATG 2 cut(s) 172, 521
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 239
CciI TCATGA 1 cut(s) 530
CfoI GCGC 2 cut(s) 122, 502
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 133
CviAII CATG 3 cut(s) 256, 262, 531
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 8
DpnI GATC 3 cut(s) 138, 143, 384
DpnII GATC 3 cut(s) 136, 141, 382
Eco24I GRGCYC 2 cut(s) 94, 274
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 87
EcoT38I GRGCYC 2 cut(s) 94, 274
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 3 cut(s) 259, 265, 534
FaiI YATR 7 cut(s) 257, 263, 326, 335, 452, 467, 532
FatI CATG 3 cut(s) 255, 261, 530
FauI CCCGC 1 cut(s) 25
FblI GTMKAC 2 cut(s) 222, 410
Fnu4HI GCNGC 3 cut(s) 329, 495, 498
FokI GGATG 2 cut(s) 179, 528
FriOI GRGCYC 2 cut(s) 94, 274
Fsp4HI GCNGC 3 cut(s) 329, 495, 498
GlaI GCGC 2 cut(s) 121, 501
GluI GCNGC 3 cut(s) 329, 495, 498
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 1 cut(s) 331
HapII CCGG 2 cut(s) 64, 164
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 122, 502
Hin1II CATG 3 cut(s) 259, 265, 534
Hin6I GCGC 2 cut(s) 120, 500
HinP1I GCGC 2 cut(s) 120, 500
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 4 cut(s) 71, 349, 396, 437
HpaII CCGG 2 cut(s) 64, 164
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 2 cut(s) 223, 411
Hpy188I TCNGA 4 cut(s) 57, 141, 179, 289
Hpy188III TCNNGA 6 cut(s) 64, 164, 299, 386, 441, 531
Hpy8I GTNNAC 2 cut(s) 223, 411
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 1 cut(s) 473
HpyCH4IV ACGT 1 cut(s) 159
HpyF3I CTNAG 1 cut(s) 8
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 3 cut(s) 259, 265, 534
HspAI GCGC 2 cut(s) 120, 500
Kpn2I TCCGGA 2 cut(s) 63, 163
Kzo9I GATC 3 cut(s) 136, 141, 382
LmnI GCTCC 3 cut(s) 57, 180, 277
LpnPI CCDG 9 cut(s) 77, 107, 177, 185, 312, 351, 390, 399, 399
Lsp1109I GCAGC 2 cut(s) 481, 484
MaeII ACGT 1 cut(s) 159
MalI GATC 3 cut(s) 138, 143, 384
MboI GATC 3 cut(s) 136, 141, 382
MboII GAAGA 2 cut(s) 94, 519
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 94, 274
MluCI AATT 2 cut(s) 51, 145
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 5, 8, 71, 223, 469
MroI TCCGGA 2 cut(s) 63, 163
MseI TTAA 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 260
MspI CCGG 2 cut(s) 64, 164
Mva1269I GAATGC 1 cut(s) 122
MvnI CGCG 1 cut(s) 120
NdeII GATC 3 cut(s) 136, 141, 382
NlaIII CATG 3 cut(s) 259, 265, 534
NlaIV GGNNCC 3 cut(s) 59, 273, 384
NspI RCATGY 1 cut(s) 259
PagI TCATGA 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 3 cut(s) 71, 349, 437
PkrI GCNGC 3 cut(s) 330, 496, 499
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
Psp1406I AACGTT 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 3 cut(s) 59, 273, 384
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 260
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 3 cut(s) 329, 495, 498
Sau3AI GATC 3 cut(s) 136, 141, 382
SchI GAGTC 1 cut(s) 390
SduI GDGCHC 2 cut(s) 94, 274
SetI ASST 8 cut(s) 51, 162, 177, 213, 239, 308, 484, 511
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 2 cut(s) 51, 145
SsiI CCGC 3 cut(s) 26, 32, 328
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 153, 222, 440
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 51, 145
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 3 cut(s) 71, 349, 437
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TscAI CASTG 1 cut(s) 199
TseI GCWGC 2 cut(s) 494, 497
TspDTI ATGAA 3 cut(s) 84, 434, 519
TspGWI ACGGA 2 cut(s) 96, 528
TspRI CASTG 1 cut(s) 199
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 222, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.