pycom09g19240

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005311
Physical Location & Seq
Forward (+)
19991787 .. 19996062
4276 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19240.1

Sequence Viewer

Length: 603 bp
ATGAGCTATTTACAATTTGGGGCAGAAGTTTCAGGAAAAACATGTAGAGAAGGAGAAAGAGATAGACACAGAGACAGCGAACTCAAAGATGGGGTCGAGAGCGAGAGCAGCCGGGGCATTACCAAACCTCATTCGGACCCTCCGGAAAGAAGCCCACCCGAAACCCACAACCCAACAATCACTCCCGTCTCTCAGACGCGCCTTCTCTCTCTACGATCGATCAATCTCATCGACAACGTCCCCGACGACCAGCTTCGCTTTCAGCGGTACACAGAGAATGGATTCACAGTGAATGGTGTGGACTACGAAGGAAGCTTGCTTTGTGTTGGAAATATGTTAATGTCTTGGGCTCCCACCAAATTGTCTGATGTCACTCCTGATAGCTTATCAATCTTCCAACTCGTGCGGCCTATTCCTGAGATTTTGATTCTCGGCTGTGGGAGAAACATTGAACCTGTAGATCCTGAACTACGACGCTACATTCGTTCTACGGGAATGAAGTTAGAAGCCGTTGACTCGAGAAACGCTATATCGACTTACAACATACTGAACGAGGAAGGTAGGATTGTGGCTGCTGCACTCGTGCCATACGGAGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

201

Amino Acids

22.21

Weight (kDa)

5.17

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 88 - 194 2.3e-33 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 199
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 2 cut(s) 265, 406
AclWI GGATC 1 cut(s) 455
AfaI GTAC 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 41
AgsI TTSAA 1 cut(s) 452
AluBI AGCT 5 cut(s) 6, 253, 315, 384, 596
AluI AGCT 5 cut(s) 6, 253, 315, 384, 596
Alw26I GTCTC 2 cut(s) 66, 193
AlwI GGATC 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 4 cut(s) 108, 572, 575, 596
ArsI GACNNNNNNTTYG 4 cut(s) 78, 110, 465, 497
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 352
BauI CACGAG 2 cut(s) 401, 581
BbvI GCAGC 4 cut(s) 120, 559, 562, 583
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 494
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 66, 193
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 5 cut(s) 109, 407, 573, 576, 597
BlsI GCNGC 5 cut(s) 110, 408, 574, 577, 598
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 138, 351
BmrFI CCNGG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
BseAI TCCGGA 1 cut(s) 142
BseCI ATCGAT 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 206, 408
BseXI GCAGC 4 cut(s) 120, 559, 562, 583
BsgI GTGCAG 1 cut(s) 561
Bsh1236I CGCG 1 cut(s) 199
Bsh1285I CGRYCG 1 cut(s) 218
BshFI GGCC 1 cut(s) 409
BshVI ATCGAT 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 2 cut(s) 112, 143
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 2 cut(s) 66, 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 352
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 3 cut(s) 215, 219, 460
BspACI CCGC 2 cut(s) 265, 406
BspANI GGCC 1 cut(s) 409
BspCNI CTCAG 2 cut(s) 205, 409
BspDI ATCGAT 1 cut(s) 218
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 1 cut(s) 199
BspLI GGNNCC 2 cut(s) 138, 351
BspPI GGATC 1 cut(s) 455
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 215, 219, 460
BssSI CACGAG 2 cut(s) 401, 581
Bst2BI CACGAG 2 cut(s) 401, 581
Bst4CI ACNGT 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 192, 417
BstFNI CGCG 1 cut(s) 199
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 3 cut(s) 218, 222, 463
BstMAI GTCTC 2 cut(s) 66, 193
BstMBI GATC 3 cut(s) 215, 219, 460
BstMCI CGRYCG 1 cut(s) 218
BstMWI GCNNNNNNNGC 2 cut(s) 108, 114
BstNSI RCATGY 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 199
BstV1I GCAGC 4 cut(s) 120, 559, 562, 583
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
Bsu15I ATCGAT 1 cut(s) 218
BsuRI GGCC 1 cut(s) 409
BsuTUI ATCGAT 1 cut(s) 218
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 317
CfoI GCGC 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 218
CseI GACGC 2 cut(s) 205, 483
Csp6I GTAC 1 cut(s) 268
CviAII CATG 1 cut(s) 42
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 192, 417
DpnI GATC 3 cut(s) 217, 221, 462
DpnII GATC 3 cut(s) 215, 219, 460
Eco24I GRGCYC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 352
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 1 cut(s) 45
FaiI YATR 5 cut(s) 43, 335, 530, 545, 589
FaqI GGGAC 1 cut(s) 224
FatI CATG 1 cut(s) 41
Fnu4HI GCNGC 5 cut(s) 109, 407, 573, 576, 597
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 5 cut(s) 109, 407, 573, 576, 597
GlaI GCGC 1 cut(s) 200
GluI GCNGC 5 cut(s) 109, 407, 573, 576, 597
HaeIII GGCC 1 cut(s) 409
HapII CCGG 2 cut(s) 112, 143
HgaI GACGC 2 cut(s) 205, 483
HhaI GCGC 1 cut(s) 201
Hin1II CATG 1 cut(s) 45
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 3 cut(s) 282, 427, 515
HpaII CCGG 2 cut(s) 112, 143
Hpy166II GTNNAC 3 cut(s) 270, 301, 514
Hpy188I TCNGA 3 cut(s) 136, 195, 367
Hpy188III TCNNGA 7 cut(s) 33, 97, 143, 377, 416, 464, 519
Hpy8I GTNNAC 3 cut(s) 270, 301, 514
Hpy99I CGWCG 2 cut(s) 248, 477
HpyAV CCTTC 4 cut(s) 44, 212, 302, 551
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 114
HpyF3I CTNAG 2 cut(s) 192, 417
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 45
HspAI GCGC 1 cut(s) 199
Kpn2I TCCGGA 1 cut(s) 142
Kzo9I GATC 3 cut(s) 215, 219, 460
LmnI GCTCC 2 cut(s) 355, 593
LpnPI CCDG 8 cut(s) 18, 125, 156, 263, 390, 429, 468, 477
Lsp1109I GCAGC 4 cut(s) 120, 559, 562, 583
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 370
MalI GATC 3 cut(s) 217, 221, 462
MboI GATC 3 cut(s) 215, 219, 460
MboII GAAGA 1 cut(s) 385
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 2 cut(s) 14, 359
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 307, 421
MnlI CCTC 3 cut(s) 138, 150, 547
MroI TCCGGA 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 2 cut(s) 338, 601
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 2 cut(s) 112, 143
MspR9I CCNGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 108, 114
NciI CCSGG 1 cut(s) 113
NdeII GATC 3 cut(s) 215, 219, 460
NlaIII CATG 1 cut(s) 45
NlaIV GGNNCC 2 cut(s) 138, 351
NmeAIII GCCGAG 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 1 cut(s) 45
PaeR7I CTCGAG 1 cut(s) 517
PciI ACATGT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 481
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 282, 427
PflFI GACNNNGTC 1 cut(s) 236
PkrI GCNGC 5 cut(s) 110, 408, 574, 577, 598
Ple19I CGATCG 1 cut(s) 218
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PscI ACATGT 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 138, 351
PspPI GGNCC 1 cut(s) 136
PsuI RGATCY 1 cut(s) 460
PsyI GACNNNGTC 1 cut(s) 236
PvuI CGATCG 1 cut(s) 218
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SaqAI TTAA 2 cut(s) 338, 601
SatI GCNGC 5 cut(s) 109, 407, 573, 576, 597
Sau3AI GATC 3 cut(s) 215, 219, 460
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 352
SetI ASST 9 cut(s) 8, 130, 240, 255, 317, 386, 457, 562, 598
SfcI CTRYAG 1 cut(s) 456
Sfr274I CTCGAG 1 cut(s) 517
SinI GGWCC 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 517
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
Sse9I AATT 2 cut(s) 14, 359
SsiI CCGC 2 cut(s) 265, 406
StyD4I CCNGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 240
TaqI TCGA 5 cut(s) 96, 218, 231, 518, 533
TasI AATT 2 cut(s) 14, 359
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 2 cut(s) 282, 427
Tru1I TTAA 2 cut(s) 338, 601
Tru9I TTAA 2 cut(s) 338, 601
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 4 cut(s) 108, 572, 575, 596
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 512
TspRI CASTG 1 cut(s) 294
Tth111I GACNNNGTC 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 136
XceI RCATGY 1 cut(s) 45
XhoI CTCGAG 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.