MD07G1082600.v1.1

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005311
Physical Location & Seq
Reverse (-)
8093215 .. 8097535
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1082600.v1.1.491

Sequence Viewer

Length: 738 bp
ATGCCACCGTTTCGCTCTTTGGCAATGGCAGGAAAAACCTCTGCCATGCCTTGCTTCCTGTCACCCCACCAATTCCAATCATCAATTCTCAACTTTCTTCAACGTTTTCAGATTTGGAATCGAACCCACGTTCGATTTTCCATTTTCTTCGATCCCCACTTGAAATTTTCAGCTAAACATAGATTTCTCCGACGAATTTCAGATATCAGAGAGGGAGAGATGGGGATGAGAGTGAGAGCCATGGGGGCACTGCCGGACCTCATTCGGAGCCTCCAGAAGGAAGCCCACCCGAAACCCGCAAACCCGCAATCGCTGCCGTCGCTGAGACGCGCCTTCTCTCTCTACGATCAGATCAATCTCATCGACAACGTCCCCAGCGACCAGCTTCGGTTTCAACGTTACACGGACACAGGGTTCACAGTGAATGGGGTGGACTATGAAGGAAGCCTACTGTGTGTTGGAAACATGTTGATGTCTTGGGCTCCCAACAAGTTCTCTCAAATCACTACTGATAGCCTATCCATCTTTCGAACCGTGCGACCTGTACCAGAGATTTTGATAATTGGCTGTGGAAGGTACATTGAACCTGTAAGTCCTGAACTACAACGCTTCATCCGGTCTACTGGAATGAAACTAGAAGCCATTGACACGAAAAATGCTGTATCGACCTACAACATACTCAATGAGGAAGGGAGGATTGTGGCTGCCGCACTTTTACCATACGGGATTTCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

246

Amino Acids

27.84

Weight (kDa)

9.78

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 132 - 239 1e-32 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 620
AccII CGCG 1 cut(s) 330
AciI CCGC 3 cut(s) 297, 305, 708
AclI AACGTT 2 cut(s) 103, 397
AclWI GGATC 1 cut(s) 146
AcsI RAATTY 2 cut(s) 164, 195
AfaI GTAC 2 cut(s) 546, 578
AfiI CCNNNNNNNGG 1 cut(s) 277
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 4 cut(s) 101, 163, 395, 584
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
AluBI AGCT 2 cut(s) 173, 385
AluI AGCT 2 cut(s) 173, 385
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 1 cut(s) 146
ApeKI GCWGC 2 cut(s) 313, 704
ApoI RAATTY 2 cut(s) 164, 195
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 54
AsuII TTCGAA 1 cut(s) 529
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 484
BarI GAAGNNNNNNTAC 2 cut(s) 432, 464
BbvI GCAGC 2 cut(s) 300, 691
BccI CCATC 2 cut(s) 214, 530
BceAI ACGGC 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 635
BglI GCCNNNNNGGC 1 cut(s) 245
BisI GCNGC 3 cut(s) 314, 705, 708
BlsI GCNGC 3 cut(s) 315, 706, 709
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 269, 483
BpmI CTGGAG 1 cut(s) 257
Bpu14I TTCGAA 1 cut(s) 529
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 615
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 628
Bse3DI GCAATG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 231, 612
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 628
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 2 cut(s) 300, 691
BseYI CCCAGC 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 330
BsiSI CCGG 2 cut(s) 254, 616
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 1 cut(s) 319
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 529
Bsp1286I GDGCHC 2 cut(s) 250, 484
Bsp143I GATC 3 cut(s) 151, 346, 351
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 3 cut(s) 297, 305, 708
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 734
BspLI GGNNCC 2 cut(s) 269, 483
BspPI GGATC 1 cut(s) 146
BspT104I TTCGAA 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 1 cut(s) 628
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 3 cut(s) 151, 346, 351
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 4 cut(s) 9, 421, 453, 535
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 529
BstDEI CTNAG 1 cut(s) 323
BstDSI CCRYGG 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 231, 612
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 154, 349, 354
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 151, 346, 351
BstMWI GCNNNNNNNGC 3 cut(s) 245, 313, 319
BstNSI RCATGY 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 300, 691
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 231, 612
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 2 cut(s) 248, 426
CciI TCATGA 1 cut(s) 734
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 256
CseI GACGC 1 cut(s) 336
Csp6I GTAC 2 cut(s) 545, 577
CviAII CATG 4 cut(s) 46, 241, 466, 735
CviQI GTAC 2 cut(s) 545, 577
DdeI CTNAG 1 cut(s) 323
DpnI GATC 3 cut(s) 153, 348, 353
DpnII GATC 3 cut(s) 151, 346, 351
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 484
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 256
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 4 cut(s) 49, 244, 469, 738
FaiI YATR 8 cut(s) 47, 180, 242, 438, 467, 677, 721, 736
FaqI GGGAC 1 cut(s) 356
FatI CATG 4 cut(s) 45, 240, 465, 734
FauI CCCGC 2 cut(s) 304, 312
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 3 cut(s) 314, 705, 708
FokI GGATG 2 cut(s) 238, 599
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 3 cut(s) 314, 705, 708
FspBI CTAG 1 cut(s) 635
GlaI GCGC 1 cut(s) 331
GluI GCNGC 3 cut(s) 314, 705, 708
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 257
HapII CCGG 2 cut(s) 254, 616
HgaI GACGC 1 cut(s) 336
HhaI GCGC 1 cut(s) 332
Hin1II CATG 4 cut(s) 49, 244, 469, 738
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 1 cut(s) 118
HpaII CCGG 2 cut(s) 254, 616
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 3 cut(s) 417, 433, 621
Hpy188I TCNGA 6 cut(s) 111, 191, 202, 209, 267, 351
Hpy188III TCNNGA 3 cut(s) 274, 596, 735
Hpy8I GTNNAC 3 cut(s) 417, 433, 621
Hpy99I CGWCG 2 cut(s) 195, 322
HpyAV CCTTC 5 cut(s) 271, 343, 434, 567, 683
HpyCH4III ACNGT 4 cut(s) 9, 421, 453, 535
HpyCH4IV ACGT 4 cut(s) 103, 129, 369, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 313, 319
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 4 cut(s) 103, 129, 369, 397
Hsp92II CATG 4 cut(s) 49, 244, 469, 738
HspAI GCGC 1 cut(s) 330
Kzo9I GATC 3 cut(s) 151, 346, 351
LmnI GCTCC 2 cut(s) 267, 487
Lsp1109I GCAGC 2 cut(s) 300, 691
MaeI CTAG 1 cut(s) 635
MaeII ACGT 4 cut(s) 103, 129, 369, 397
MaeIII GTNAC 2 cut(s) 60, 398
MalI GATC 3 cut(s) 153, 348, 353
MboI GATC 3 cut(s) 151, 346, 351
MboII GAAGA 2 cut(s) 89, 139
MhlI GDGCHC 2 cut(s) 250, 484
MluCI AATT 5 cut(s) 71, 84, 164, 195, 561
MmeI TCCRAC 2 cut(s) 214, 439
MnlI CCTC 6 cut(s) 49, 205, 269, 281, 679, 687
MslI CAYNNNNRTG 1 cut(s) 470
MspI CCGG 2 cut(s) 254, 616
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 245, 313, 319
NcoI CCATGG 1 cut(s) 240
NdeII GATC 3 cut(s) 151, 346, 351
NlaIII CATG 4 cut(s) 49, 244, 469, 738
NlaIV GGNNCC 2 cut(s) 269, 483
NmuCI GTSAC 1 cut(s) 60
NspI RCATGY 1 cut(s) 469
NspV TTCGAA 1 cut(s) 529
PagI TCATGA 1 cut(s) 734
PciI ACATGT 1 cut(s) 465
PcsI WCGNNNNNNNCGW 3 cut(s) 375, 394, 535
PfeI GAWTC 1 cut(s) 118
PflFI GACNNNGTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 315, 706, 709
PscI ACATGT 1 cut(s) 465
Psp1406I AACGTT 2 cut(s) 103, 397
PspFI CCCAGC 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 269, 483
PspPI GGNCC 1 cut(s) 256
PsyI GACNNNGTC 1 cut(s) 368
RsaI GTAC 2 cut(s) 546, 578
RsaNI GTAC 2 cut(s) 545, 577
RseI CAYNNNNRTG 1 cut(s) 470
SatI GCNGC 3 cut(s) 314, 705, 708
Sau3AI GATC 3 cut(s) 151, 346, 351
Sau96I GGNCC 1 cut(s) 256
SduI GDGCHC 2 cut(s) 250, 484
SfuI TTCGAA 1 cut(s) 529
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 470
Sse9I AATT 5 cut(s) 71, 84, 164, 195, 561
SsiI CCGC 3 cut(s) 297, 305, 708
SspMI CTAG 1 cut(s) 635
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 4 cut(s) 9, 421, 453, 535
TaiI ACGT 4 cut(s) 106, 132, 372, 400
TaqI TCGA 6 cut(s) 121, 133, 150, 363, 529, 665
TasI AATT 5 cut(s) 71, 84, 164, 195, 561
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 1 cut(s) 118
TscAI CASTG 2 cut(s) 255, 426
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 2 cut(s) 313, 704
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 3 cut(s) 453, 601, 644
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 255, 426
Tth111I GACNNNGTC 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 2 cut(s) 164, 195
XceI RCATGY 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 620
XspI CTAG 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.