Rw3G024870

NADH dehydrogenase ubiquinone 1 alpha subcomplex assembly factor 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005311
Physical Location & Seq
Reverse (-)
32478035 .. 32482865
4831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024870.1

Sequence Viewer

Length: 534 bp
ATGGGGTTGAGAGGAGGAGGAGGAGCAGTGGCGGGGGCATTACCGAACCTGATTCGGAGCCTCCGAAAGGAAGCCCACCCGAAGACCCGAGCCCAACAATCACTACCGTCTCTCAGGCGGGCCTTCTCCCTGTACGATCAGATCAATCTGATCGACAACGTTCCGGATGACCAGCTTCGATTTCAAGGGTACACCGACACAGGTTTCAAAGTGAATGGGGTCGACTACGAAGGCAGCTTGCTTTGTATTGGCAACATGCTCATGTCTTGGGCTCCCAAAAAATTCTCCGATATCACTCCTGACAGCTTATCAATGTTCCAACTTATGCGCCCTATACCAGAGATTTTAATTCTTGGCTGTGGGAGATACATTGAACCCGTAGATCCTGAAGTACGACGCTTCATTCGGTCTACTGGAATGAAGTTAGAAGCCGTTGACTCGAGAAACGCCATATCAACTTACAACATACTGAATGAGGAAGGTAGGATCGTGGCTGCTGCACTTTTACCTTACGGATGCGGAGTTTTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
DUF498

Protein Analysis

177

Amino Acids

19.49

Weight (kDa)

8.47

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF498 PF04430 62 - 169 6.9e-34 Protein of unknown function (DUF498/DUF598)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 222, 410
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 3 cut(s) 32, 118, 519
AclI AACGTT 1 cut(s) 159
AclWI GGATC 2 cut(s) 377, 494
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 134, 191, 393
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 3 cut(s) 185, 208, 374
AluBI AGCT 3 cut(s) 175, 237, 306
AluI AGCT 3 cut(s) 175, 237, 306
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 2 cut(s) 377, 494
Ama87I CYCGRG 2 cut(s) 87, 439
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 3 cut(s) 234, 494, 497
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 120
AvaI CYCGRG 2 cut(s) 87, 439
BanII GRGCYC 2 cut(s) 94, 274
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 3 cut(s) 246, 481, 484
BceAI ACGGC 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 114
BisI GCNGC 3 cut(s) 235, 495, 498
BlsI GCNGC 3 cut(s) 236, 496, 499
BmeT110I CYCGRG 2 cut(s) 87, 439
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 2 cut(s) 59, 273
BmsI GCATC 1 cut(s) 506
BpiI GAAGAC 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 418
BseAI TCCGGA 1 cut(s) 163
BseGI GGATG 2 cut(s) 172, 521
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 4 cut(s) 27, 30, 33, 36
BseXI GCAGC 3 cut(s) 246, 481, 484
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 1 cut(s) 122
BsiHKCI CYCGRG 2 cut(s) 87, 439
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 114
BsmBI CGTCTC 1 cut(s) 114
BsnI GGCC 1 cut(s) 122
BsoBI CYCGRG 2 cut(s) 87, 439
Bsp1286I GDGCHC 2 cut(s) 94, 274
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 136, 141, 150, 382, 486
BspACI CCGC 3 cut(s) 32, 118, 519
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 163
BspHI TCATGA 1 cut(s) 530
BspLI GGNNCC 2 cut(s) 59, 273
BspPI GGATC 2 cut(s) 377, 494
BsrI ACTGG 1 cut(s) 418
BssMI GATC 5 cut(s) 136, 141, 150, 382, 486
Bst4CI ACNGT 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 120, 239
BstDEI CTNAG 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 172, 521
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 5 cut(s) 139, 144, 153, 385, 489
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 5 cut(s) 136, 141, 150, 382, 486
BstNSI RCATGY 1 cut(s) 259
BstV1I GCAGC 3 cut(s) 246, 481, 484
BstV2I GAAGAC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 1 cut(s) 122
BtsCI GGATG 2 cut(s) 172, 521
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 2 cut(s) 120, 239
CciI TCATGA 1 cut(s) 530
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 120
CseI GACGC 1 cut(s) 405
Csp6I GTAC 3 cut(s) 133, 190, 392
CviAII CATG 3 cut(s) 256, 262, 531
CviQI GTAC 3 cut(s) 133, 190, 392
DdeI CTNAG 1 cut(s) 113
DpnI GATC 5 cut(s) 138, 143, 152, 384, 488
DpnII GATC 5 cut(s) 136, 141, 150, 382, 486
Eco24I GRGCYC 2 cut(s) 94, 274
Eco32I GATATC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 2 cut(s) 87, 439
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoRV GATATC 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 94, 274
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 3 cut(s) 259, 265, 534
FaiI YATR 7 cut(s) 257, 263, 326, 335, 452, 467, 532
FatI CATG 3 cut(s) 255, 261, 530
FauI CCCGC 2 cut(s) 25, 111
FblI GTMKAC 2 cut(s) 222, 410
Fnu4HI GCNGC 3 cut(s) 235, 495, 498
FokI GGATG 2 cut(s) 179, 528
FriOI GRGCYC 2 cut(s) 94, 274
Fsp4HI GCNGC 3 cut(s) 235, 495, 498
GlaI GCGC 1 cut(s) 329
GluI GCNGC 3 cut(s) 235, 495, 498
HaeIII GGCC 1 cut(s) 122
HapII CCGG 1 cut(s) 164
HgaI GACGC 1 cut(s) 405
HhaI GCGC 1 cut(s) 330
Hin1II CATG 3 cut(s) 259, 265, 534
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 2 cut(s) 223, 436
HindII GTYRAC 2 cut(s) 223, 436
HinfI GANTC 2 cut(s) 52, 437
HpaII CCGG 1 cut(s) 164
Hpy166II GTNNAC 4 cut(s) 192, 223, 411, 436
Hpy188I TCNGA 5 cut(s) 57, 65, 141, 150, 289
Hpy188III TCNNGA 5 cut(s) 164, 299, 386, 441, 531
Hpy8I GTNNAC 4 cut(s) 192, 223, 411, 436
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 3 cut(s) 133, 224, 473
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 1 cut(s) 500
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 3 cut(s) 259, 265, 534
HspAI GCGC 1 cut(s) 328
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 5 cut(s) 136, 141, 150, 382, 486
LmnI GCTCC 3 cut(s) 23, 57, 277
Lsp1109I GCAGC 3 cut(s) 246, 481, 484
LweI GCATC 1 cut(s) 506
MaeII ACGT 1 cut(s) 159
MalI GATC 5 cut(s) 138, 143, 152, 384, 488
MboI GATC 5 cut(s) 136, 141, 150, 382, 486
MboII GAAGA 1 cut(s) 94
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 94, 274
MluCI AATT 2 cut(s) 281, 348
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 6 cut(s) 5, 8, 11, 14, 71, 469
MroI TCCGGA 1 cut(s) 163
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 260
MspI CCGG 1 cut(s) 164
NdeII GATC 5 cut(s) 136, 141, 150, 382, 486
NlaIII CATG 3 cut(s) 259, 265, 534
NlaIV GGNNCC 2 cut(s) 59, 273
NspI RCATGY 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 439
PagI TCATGA 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 3 cut(s) 236, 496, 499
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
Psp1406I AACGTT 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 59, 273
PspPI GGNCC 1 cut(s) 120
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 3 cut(s) 134, 191, 393
RsaNI GTAC 3 cut(s) 133, 190, 392
RseI CAYNNNNRTG 1 cut(s) 260
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 3 cut(s) 235, 495, 498
Sau3AI GATC 5 cut(s) 136, 141, 150, 382, 486
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 2 cut(s) 94, 274
SetI ASST 8 cut(s) 51, 162, 177, 205, 239, 308, 484, 511
SfaNI GCATC 1 cut(s) 506
Sfr274I CTCGAG 1 cut(s) 439
SlaI CTCGAG 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 2 cut(s) 281, 348
SsiI CCGC 3 cut(s) 32, 118, 519
TaaI ACNGT 1 cut(s) 108
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 153, 178, 222, 440
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 281, 348
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 1 cut(s) 33
TseI GCWGC 3 cut(s) 234, 494, 497
TspDTI ATGAA 2 cut(s) 391, 434
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 1 cut(s) 33
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 439
XmiI GTMKAC 2 cut(s) 222, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.