Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008440

Physical Location & Seq

Genomic Coordinates Reverse (-)

19080254 .. 19081751

Gene Structure 1498 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G46493.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 555 bp

ATGCTTCCTTCTTGTCAAATCGTGAAGAGATTACATGTTCCGGCTACTTCGCCATTTGGGGAAACAAGATTCTCGAGTGACCTCAACAATCAAAGCCTCCTGCTGGAGTGGGCCTTGCCTGATTGCAGAGCCAAATGCTTAGGGGCTACTAAAAAAACTGGTAATTTAAGTAGTGGAGTACTAGTACTAGTTATAAGCTTGTCAGCAGTCACCGTCTTTGTCTTTCCTACTTGTATCGCTATCCGCCTTTACGACTCCGAAAGATTCGATTCAGCTATCGCAGCCGCAACCGTAATGCAGCAGCCGAGAGAGGTTATGGCCAGGAGGGGTCTTGACCAATCTACAATAGAAACGTTCAAGAAAATGGAGTTGGGAGAAAGCAGAAGGCTTTCGGGTACTAATGGTATTGTGTGTCCCATTTGTTTATCAGAGTATGCGAGCAAAGAGACCGTACGTTTCATACCGGAATGTGACCATTGCTTCCATGTGGAATGTATTGATGTGTGGCTCAAGATTCATGGTTCATGTCCTCTTTGTCGGAACTCATGTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

184

Amino Acids

20.32

Weight (kDa)

7.98

Isoelectric Point (pI)

57.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	138 - 180	2e-13	Ring finger domain
zf-RING_11	PF17123	138 - 166	8.6e-08	RING-like zinc finger
zf-rbx1	PF12678	138 - 180	1.7e-07	RING-H2 zinc finger domain
zf-C3HC4_2	PF13923	138 - 179	1.1e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008440)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca	FvH4_7g16350
malus_domestica	MD07G1148900.v1.1
prunus_persica	Prupe.2G187400_v2.0.a1
pyrus_communis	pycom07g14790
rosa_chinensis	RchiOBHm_Chr1g0359361
rosa_laevigata	RLG00000027876
rosa_multiflora	Rmu_ssc0000358.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297050
rosa_rugosa	Rorug01G0270500
rosa_samantha	Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana	Rw1G025210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	194
AciI	CCGC	2 cut(s)	244, 285
AclI	AACGTT	1 cut(s)	353
AcoI	YGGCCR	1 cut(s)	318
AfaI	GTAC	4 cut(s)	180, 186, 397, 453
AfiI	CCNNNNNNNGG	1 cut(s)	103
AflIII	ACRYGT	1 cut(s)	34
AgsI	TTSAA	1 cut(s)	358
AhlI	ACTAGT	2 cut(s)	181, 187
AjnI	CCWGG	1 cut(s)	320
AjuI	GAANNNNNNNTTGG	2 cut(s)	353, 385
AluBI	AGCT	2 cut(s)	198, 275
AluI	AGCT	2 cut(s)	198, 275
Alw26I	GTCTC	1 cut(s)	440
Ama87I	CYCGRG	1 cut(s)	73
AoxI	GGCC	2 cut(s)	111, 318
ApeKI	GCWGC	3 cut(s)	281, 298, 301
Asp700I	GAANNNNTTC	1 cut(s)	388
AspS9I	GGNCC	1 cut(s)	111
AsuHPI	GGTGA	1 cut(s)	202
AvaI	CYCGRG	1 cut(s)	73
BalI	TGGCCA	1 cut(s)	320
BbvI	GCAGC	3 cut(s)	293, 310, 313
BciT130I	CCWGG	1 cut(s)	322
BcoDI	GTCTC	1 cut(s)	440
BcuI	ACTAGT	2 cut(s)	181, 187
BfaI	CTAG	2 cut(s)	182, 188
BisI	GCNGC	4 cut(s)	282, 285, 299, 302
BlsI	GCNGC	4 cut(s)	283, 286, 300, 303
BmcAI	AGTACT	2 cut(s)	180, 186
Bme1390I	CCNGG	1 cut(s)	322
BmeT110I	CYCGRG	1 cut(s)	73
BmgT120I	GGNCC	1 cut(s)	111
BmrFI	CCNGG	1 cut(s)	322
BpmI	CTGGAG	1 cut(s)	125
Bpu10I	CCTNAGC	1 cut(s)	139
BpuEI	CTTGAG	1 cut(s)	494
BsaI	GGTCTC	1 cut(s)	440
BsaWI	WCCGGW	1 cut(s)	463
BsaXI	ACNNNNNCTCC	2 cut(s)	168, 198
Bsc4I	CCNNNNNNNGG	1 cut(s)	103
Bse1I	ACTGG	1 cut(s)	163
Bse3DI	GCAATG	1 cut(s)	475
BseBI	CCWGG	1 cut(s)	322
BseLI	CCNNNNNNNGG	1 cut(s)	103
BseMI	GCAATG	1 cut(s)	475
BseNI	ACTGG	1 cut(s)	163
BseXI	GCAGC	3 cut(s)	293, 310, 313
BshFI	GGCC	2 cut(s)	113, 320
BsiHKCI	CYCGRG	1 cut(s)	73
BsiSI	CCGG	2 cut(s)	41, 464
BsiWI	CGTACG	1 cut(s)	451
BslFI	GGGAC	1 cut(s)	399
BslI	CCNNNNNNNGG	1 cut(s)	103
BsmAI	GTCTC	1 cut(s)	440
BsmFI	GGGAC	1 cut(s)	399
BsnI	GGCC	2 cut(s)	113, 320
Bso31I	GGTCTC	1 cut(s)	440
BsoBI	CYCGRG	1 cut(s)	73
BspACI	CCGC	2 cut(s)	244, 285
BspANI	GGCC	2 cut(s)	113, 320
BspTNI	GGTCTC	1 cut(s)	440
BsrDI	GCAATG	1 cut(s)	475
BsrI	ACTGG	1 cut(s)	163
Bst2UI	CCWGG	1 cut(s)	322
Bst4CI	ACNGT	3 cut(s)	214, 292, 451
Bst6I	CTCTTC	1 cut(s)	20
BstC8I	GCNNGC	1 cut(s)	439
BstDEI	CTNAG	1 cut(s)	139
BstMAI	GTCTC	1 cut(s)	440
BstMWI	GCNNNNNNNGC	1 cut(s)	281
BstNI	CCWGG	1 cut(s)	322
BstNSI	RCATGY	1 cut(s)	38
BstSCI	CCNGG	1 cut(s)	320
BstV1I	GCAGC	3 cut(s)	293, 310, 313
BsuRI	GGCC	2 cut(s)	113, 320
Cac8I	GCNNGC	1 cut(s)	439
Cfr13I	GGNCC	1 cut(s)	111
Csp6I	GTAC	4 cut(s)	179, 185, 396, 452
CviAII	CATG	5 cut(s)	35, 485, 518, 525, 546
CviQI	GTAC	4 cut(s)	179, 185, 396, 452
DdeI	CTNAG	1 cut(s)	139
EaeI	YGGCCR	1 cut(s)	318
Eam1104I	CTCTTC	1 cut(s)	20
EarI	CTCTTC	1 cut(s)	20
EciI	GGCGGA	1 cut(s)	233
Eco31I	GGTCTC	1 cut(s)	440
Eco88I	CYCGRG	1 cut(s)	73
EcoRII	CCWGG	1 cut(s)	320
FaeI	CATG	5 cut(s)	38, 488, 521, 528, 549
FaiI	YATR	9 cut(s)	36, 194, 317, 435, 461, 486, 519, 526, 547
FaqI	GGGAC	1 cut(s)	399
FatI	CATG	5 cut(s)	34, 484, 517, 524, 545
Fnu4HI	GCNGC	4 cut(s)	282, 285, 299, 302
Fsp4HI	GCNGC	4 cut(s)	282, 285, 299, 302
FspBI	CTAG	2 cut(s)	182, 188
GluI	GCNGC	4 cut(s)	282, 285, 299, 302
GsuI	CTGGAG	1 cut(s)	125
HaeIII	GGCC	2 cut(s)	113, 320
HapII	CCGG	2 cut(s)	41, 464
Hin1II	CATG	5 cut(s)	38, 488, 521, 528, 549
HindIII	AAGCTT	1 cut(s)	196
HinfI	GANTC	5 cut(s)	69, 254, 264, 269, 514
HpaII	CCGG	2 cut(s)	41, 464
HphI	GGTGA	1 cut(s)	202
Hpy188I	TCNGA	3 cut(s)	259, 430, 540
Hpy188III	TCNNGA	5 cut(s)	22, 73, 332, 358, 511
HpyAV	CCTTC	2 cut(s)	18, 378
HpyCH4III	ACNGT	3 cut(s)	214, 292, 451
HpyCH4IV	ACGT	2 cut(s)	353, 454
HpyCH4V	TGCA	2 cut(s)	126, 298
HpyF10VI	GCNNNNNNNGC	1 cut(s)	281
HpyF3I	CTNAG	1 cut(s)	139
HpySE526I	ACGT	2 cut(s)	353, 454
Hsp92II	CATG	5 cut(s)	38, 488, 521, 528, 549
LpnPI	CCDG	8 cut(s)	54, 89, 113, 132, 144, 307, 334, 477
Lsp1109I	GCAGC	3 cut(s)	293, 310, 313
MaeI	CTAG	2 cut(s)	182, 188
MaeII	ACGT	2 cut(s)	353, 454
MaeIII	GTNAC	3 cut(s)	77, 208, 470
MboII	GAAGA	1 cut(s)	37
MlsI	TGGCCA	1 cut(s)	320
MluCI	AATT	1 cut(s)	163
MluNI	TGGCCA	1 cut(s)	320
MlyI	GAGTC	1 cut(s)	248
MmeI	TCCRAC	1 cut(s)	518
MnlI	CCTC	5 cut(s)	92, 107, 304, 318, 540
Mox20I	TGGCCA	1 cut(s)	320
MroXI	GAANNNNTTC	1 cut(s)	388
MscI	TGGCCA	1 cut(s)	320
MseI	TTAA	1 cut(s)	167
MslI	CAYNNNNRTG	1 cut(s)	550
Msp20I	TGGCCA	1 cut(s)	320
MspI	CCGG	2 cut(s)	41, 464
MspR9I	CCNGG	1 cut(s)	322
MvaI	CCWGG	1 cut(s)	322
MwoI	GCNNNNNNNGC	1 cut(s)	281
NlaIII	CATG	5 cut(s)	38, 488, 521, 528, 549
NmeAIII	GCCGAG	1 cut(s)	330
NmuCI	GTSAC	3 cut(s)	77, 208, 470
NspI	RCATGY	1 cut(s)	38
PaeR7I	CTCGAG	1 cut(s)	73
PciI	ACATGT	1 cut(s)	34
PdmI	GAANNNNTTC	1 cut(s)	388
PfeI	GAWTC	4 cut(s)	69, 264, 269, 514
Pfl23II	CGTACG	1 cut(s)	451
PkrI	GCNGC	4 cut(s)	283, 286, 300, 303
PleI	GAGTC	1 cut(s)	248
PpsI	GAGTC	1 cut(s)	248
PscI	ACATGT	1 cut(s)	34
PsiI	TTATAA	1 cut(s)	194
Psp1406I	AACGTT	1 cut(s)	353
Psp6I	CCWGG	1 cut(s)	320
PspGI	CCWGG	1 cut(s)	320
PspLI	CGTACG	1 cut(s)	451
PspPI	GGNCC	1 cut(s)	111
RsaI	GTAC	4 cut(s)	180, 186, 397, 453
RsaNI	GTAC	4 cut(s)	179, 185, 396, 452
RseI	CAYNNNNRTG	1 cut(s)	550
SaqAI	TTAA	1 cut(s)	167
SatI	GCNGC	4 cut(s)	282, 285, 299, 302
Sau96I	GGNCC	1 cut(s)	111
ScaI	AGTACT	2 cut(s)	180, 186
SchI	GAGTC	1 cut(s)	248
ScrFI	CCNGG	1 cut(s)	322
SetI	ASST	6 cut(s)	84, 200, 277, 315, 356, 457
Sfr274I	CTCGAG	1 cut(s)	73
SlaI	CTCGAG	1 cut(s)	73
SmiMI	CAYNNNNRTG	1 cut(s)	550
SmlI	CTYRAG	2 cut(s)	73, 509
SmoI	CTYRAG	2 cut(s)	73, 509
SpeI	ACTAGT	2 cut(s)	181, 187
Sse9I	AATT	1 cut(s)	163
SsiI	CCGC	2 cut(s)	244, 285
SspMI	CTAG	2 cut(s)	182, 188
StyD4I	CCNGG	1 cut(s)	320
TaaI	ACNGT	3 cut(s)	214, 292, 451
TaiI	ACGT	2 cut(s)	356, 457
TaqI	TCGA	2 cut(s)	74, 267
TasI	AATT	1 cut(s)	163
TatI	WGTACW	2 cut(s)	178, 184
TauI	GCSGC	1 cut(s)	287
TfiI	GAWTC	4 cut(s)	69, 264, 269, 514
Tru1I	TTAA	1 cut(s)	167
Tru9I	TTAA	1 cut(s)	167
TseFI	GTSAC	3 cut(s)	77, 208, 470
TseI	GCWGC	3 cut(s)	281, 298, 301
Tsp45I	GTSAC	3 cut(s)	77, 208, 470
TspDTI	ATGAA	3 cut(s)	448, 506, 513
XceI	RCATGY	1 cut(s)	38
XhoI	CTCGAG	1 cut(s)	73
XmnI	GAANNNNTTC	1 cut(s)	388
XspI	CTAG	2 cut(s)	182, 188
ZrmI	AGTACT	2 cut(s)	180, 186

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.